ATG18 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	ATG18
Entrez Gene ID	850577
Full Name	phosphoinositide binding protein ATG18
Synonyms	AUT10,CVT18,NMR1,SVP1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179986.1	NP_444297.1	phosphoinositide binding protein ATG18

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	WIPI2	XP_850630.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005910	XP_315940.3
Eremothecium gossypii	AGOS_AAL119W	NP_982423.1
Pan troglodytes (chimpanzee)	WIPI2	XP_003318335.1
Bos taurus (cattle)	WIPI2	NP_001039594.1
Drosophila melanogaster (fruit fly)	Atg18	NP_001261565.1
Kluyveromyces lactis	KLLA0D04664g	XP_453268.1
Schizosaccharomyces pombe (fission yeast)	atg18	NP_594055.1
Magnaporthe oryzae (rice blast fungus)	MGG_03139	XP_003716858.1
Mus musculus (house mouse)	Wipi2	NP_848485.1
Rattus norvegicus (Norway rat)	Wipi2	NP_001007616.1
Gallus gallus (chicken)	WIPI2	NP_001006162.1
Neurospora crassa	NCU03441	XP_956727.1
Oryza sativa (rice)	Os02g0791800	NP_001048361.1
Homo sapiens (human)	WIPI2	NP_057087.2
Saccharomyces cerevisiae (baker's yeast)	ATG18	NP_444297.1
Arabidopsis thaliana (thale cress)	G18B	NP_194780.2
Xenopus tropicalis (tropical clawed frog)	LOC100497399	XP_004917971.1
Caenorhabditis elegans (roundworm)	atg-18	NP_741576.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Murakami Y, Naitou M, Hagiwara H, Shibata T, Ozawa M, Sasanuma S, Sasanuma M, Tsuchiya Y, Soeda E, Yokoyama K
Nature genetics10(3)261-8(1995 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ATG18 and FAB1 are involved in dehydration stress tolerance in Saccharomyces cerevisiae.
Title: ATG18 and FAB1 are involved in dehydration stress tolerance in Saccharomyces cerevisiae.

The Atg2-Atg18 complex and Atg9 localized to the edge of the IM, appearing as two or three dots, in close proximity to the endoplasmic reticulum exit sites.
Title: Fine mapping of autophagy-related proteins during autophagosome formation in Saccharomyces cerevisiae.

Data provide a model whereby the Atg18 beta-propeller provides organelle specificity by binding to two determinants on the target membrane.
Title: Atg18 function in autophagy is regulated by specific sites within its ?-propeller.

effective targeting of Atg2 to the PAS can compensate for loss of Atg18 function in autophagy
Title: Autophagosome formation can be achieved in the absence of Atg18 by expressing engineered PAS-targeted Atg2.

Deletion of ATG18, ATG21 and YGR223c, alone or simultaneously has no obvious function on the MVB-pathway and carboxypeptidase Y sorting.
Title: Dissecting the localization and function of Atg18, Atg21 and Ygr223c.

The following ATG18 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ATG18 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00257	NM_001179986.1 Latest version!	Saccharomyces cerevisiae S288C phosphoinositide binding protein ATG18 (ATG18), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ATG18 ( NM_001179986.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_444297.1 Saccharomyces cerevisiae S288C phosphoinositide binding protein ATG18 (ATG18), partial mRNA.

Price & Availability↑

CloneID	OSi00257
Clone ID Related Accession (Same CDS sequence)	NM_001179986.1
Accession Version	NM_001179986.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	phosphoinositide binding protein ATG18
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001138). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCTGATT CATCACCTAC TATCAACTTT ATTAATTTCA ATCAAACCGG AACGTGTATT 
TCCCTTGGAA CGTCGAAAGG TTTCAAAATA TTCAATTGTG AGCCCTTCGG AAAATTTTAT 
TCAGAGGACA GTGGGGGCTA TGCTATCGTC GAGATGTTGT TCTCCACCTC GTTACTAGCC 
CTCGTTGGGA TAGGCGATCA ACCTGCGCTT TCACCAAGGA GATTGCGTAT AATCAACACA 
AAAAAACATT CTATTATCTG TGAGGTGACT TTCCCTACTT CTATTCTGAG TGTGAAAATG 
AATAAGTCTC GATTGGTGGT ACTTTTACAA GAGCAGATTT ATATTTATGA TATCAACACC 
ATGAGACTAT TGCATACTAT AGAAACAAAC CCTAACCCAC GTGGCCTTAT GGCTATGTCT 
CCTTCGGTAG CCAACAGCTA TTTAGTGTAT CCATCACCAC CAAAAGTTAT TAACTCCGAA 
ATTAAAGCTC ATGCCACCAC AAACAATATC ACATTGTCAG TTGGTGGCAA CACAGAGACC 
AGTTTCAAGA GAGATCAGCA AGATGCTGGC CATAGTGACA TTAGCGACTT GGATCAGTAT 
TCGAGCTTTA CTAAGAGGGA TGATGCGGAT CCAACAAGCA GTAACGGCGG TAACAGCAGT 
ATAATAAAGA ATGGTGATGT GATCGTATTC AACTTGGAAA CATTACAGCC AACCATGGTC 
ATCGAAGCTC ATAAGGGCGA GATTGCTGCA ATGGCAATTA GTTTTGATGG GACACTAATG 
GCTACCGCCT CTGATAAAGG TACTATCATC AGGGTCTTTG ACATTGAAAC GGGTGATAAG 
ATCTACCAAT TCAGGAGAGG GACGTACGCG ACAAGAATTT ACTCCATATC ATTCAGTGAA 
GATAGCCAGT ACTTGGCGGT TACCGGCTCT TCCAAAACCG TGCATATCTT CAAATTGGGG 
CATTCAATGA GCAACAATAA ACTAGACAGC GATGATAGCA ACATGGAAGA AGCTGCAGCC 
GATGATTCAT CGCTCGATAC CACCAGTATC GATGCGCTGA GTGACGAGGA AAACCCGACA 
AGACTCGCAA GAGAACCATA TGTGGATGCA TCAAGAAAGA CAATGGGTAG GATGATACGT 
TACTCTTCTC AAAAGCTATC CCGAAGAGCT GCCAGAACAT TGGGTCAGAT TTTCCCCATC 
AAAGTTACAT CGTTGTTGGA ATCCTCGCGC CATTTTGCGT CTTTGAAACT TCCCGTTGAA 
ACCAATTCCC ATGTAATGAC CATATCAAGT ATAGGCTCTC CAATAGATAT AGACACATCC 
GAGTATCCGG AACTCTTCGA AACTGGCAAT TCCGCAAGTA CAGAGTCCTA CCATGAGCCT 
GTTATGAAGA TGGTCCCCAT CAGGGTCGTT TCCTCGGATG GATACCTATA CAACTTTGTT 
ATGGACCCGG AGAGAGGCGG CGATTGCTTA ATATTGTCAC AGTATTCCAT CTTGATGGAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_444297.1
CDS	1..1503
Translation	MSDSSPTINFINFNQTGTCISLGTSKGFKIFNCEPFGKFYSEDSGGYAIVEMLFSTSLLALVGIGDQPALSPRRLRIINTKKHSIICEVTFPTSILSVKMNKSRLVVLLQEQIYIYDINTMRLLHTIETNPNPRGLMAMSPSVANSYLVYPSPPKVINSEIKAHATTNNITLSVGGNTETSFKRDQQDAGHSDISDLDQYSSFTKRDDADPTSSNGGNSSIIKNGDVIVFNLETLQPTMVIEAHKGEIAAMAISFDGTLMATASDKGTIIRVFDIETGDKIYQFRRGTYATRIYSISFSEDSQYLAVTGSSKTVHIFKLGHSMSNNKLDSDDSNMEEAAADDSSLDTTSIDALSDEENPTRLAREPYVDASRKTMGRMIRYSSQKLSRRAARTLGQIFPIKVTSLLESSRHFASLKLPVETNSHVMTISSIGSPIDIDTSEYPELFETGNSASTESYHEPVMKMVPIRVVSSDGYLYNFVMDPERGGDCLILSQYSILMD

Target ORF information:

RefSeq Version	NM_001179986.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C phosphoinositide binding protein ATG18 (ATG18), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179986.1

ORF Insert Sequence:

ATGTCTGATT CATCACCTAC TATCAACTTT ATTAATTTCA ATCAAACCGG AACGTGTATT 
TCCCTTGGAA CGTCGAAAGG TTTCAAAATA TTCAATTGTG AGCCCTTCGG AAAATTTTAT 
TCAGAGGACA GTGGGGGCTA TGCTATCGTC GAGATGTTGT TCTCCACCTC GTTACTAGCC 
CTCGTTGGGA TAGGCGATCA ACCTGCGCTT TCACCAAGGA GATTGCGTAT AATCAACACA 
AAAAAACATT CTATTATCTG TGAGGTGACT TTCCCTACTT CTATTCTGAG TGTGAAAATG 
AATAAGTCTC GATTGGTGGT ACTTTTACAA GAGCAGATTT ATATTTATGA TATCAACACC 
ATGAGACTAT TGCATACTAT AGAAACAAAC CCTAACCCAC GTGGCCTTAT GGCTATGTCT 
CCTTCGGTAG CCAACAGCTA TTTAGTGTAT CCATCACCAC CAAAAGTTAT TAACTCCGAA 
ATTAAAGCTC ATGCCACCAC AAACAATATC ACATTGTCAG TTGGTGGCAA CACAGAGACC 
AGTTTCAAGA GAGATCAGCA AGATGCTGGC CATAGTGACA TTAGCGACTT GGATCAGTAT 
TCGAGCTTTA CTAAGAGGGA TGATGCGGAT CCAACAAGCA GTAACGGCGG TAACAGCAGT 
ATAATAAAGA ATGGTGATGT GATCGTATTC AACTTGGAAA CATTACAGCC AACCATGGTC 
ATCGAAGCTC ATAAGGGCGA GATTGCTGCA ATGGCAATTA GTTTTGATGG GACACTAATG 
GCTACCGCCT CTGATAAAGG TACTATCATC AGGGTCTTTG ACATTGAAAC GGGTGATAAG 
ATCTACCAAT TCAGGAGAGG GACGTACGCG ACAAGAATTT ACTCCATATC ATTCAGTGAA 
GATAGCCAGT ACTTGGCGGT TACCGGCTCT TCCAAAACCG TGCATATCTT CAAATTGGGG 
CATTCAATGA GCAACAATAA ACTAGACAGC GATGATAGCA ACATGGAAGA AGCTGCAGCC 
GATGATTCAT CGCTCGATAC CACCAGTATC GATGCGCTGA GTGACGAGGA AAACCCGACA 
AGACTCGCAA GAGAACCATA TGTGGATGCA TCAAGAAAGA CAATGGGTAG GATGATACGT 
TACTCTTCTC AAAAGCTATC CCGAAGAGCT GCCAGAACAT TGGGTCAGAT TTTCCCCATC 
AAAGTTACAT CGTTGTTGGA ATCCTCGCGC CATTTTGCGT CTTTGAAACT TCCCGTTGAA 
ACCAATTCCC ATGTAATGAC CATATCAAGT ATAGGCTCTC CAATAGATAT AGACACATCC 
GAGTATCCGG AACTCTTCGA AACTGGCAAT TCCGCAAGTA CAGAGTCCTA CCATGAGCCT 
GTTATGAAGA TGGTCCCCAT CAGGGTCGTT TCCTCGGATG GATACCTATA CAACTTTGTT 
ATGGACCCGG AGAGAGGCGG CGATTGCTTA ATATTGTCAC AGTATTCCAT CTTGATGGAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Nature genetics10(3)261-8(1995 Jul)
Murakami Y,Naitou M,Hagiwara H,Shibata T,Ozawa M,Sasanuma S,Sasanuma M,Tsuchiya Y,Soeda E,Yokoyama K

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ATG18 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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