NIC96 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	NIC96
Entrez Gene ID	850552
Full Name	linker nucleoporin NIC96
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179967.1	NP_116657.1	linker nucleoporin NIC96

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003459	XP_311745.5
Pan troglodytes (chimpanzee)	NUP93	XP_510982.3
Canis lupus familiaris (dog)	NUP93	XP_005617597.1
Bos taurus (cattle)	NUP93	NP_001092391.1
Schizosaccharomyces pombe (fission yeast)	nup97	NP_588469.1
Arabidopsis thaliana (thale cress)	AT3G57350	NP_191294.5
Gallus gallus (chicken)	NUP93	NP_001012544.2
Danio rerio (zebrafish)	nup93	NP_571056.2
Neurospora crassa	NCU01702	XP_956908.1
Arabidopsis thaliana (thale cress)	AT2G41620	NP_565951.1
Homo sapiens (human)	NUP93	NP_055484.3
Kluyveromyces lactis	KLLA0E14191g	XP_454590.1
Eremothecium gossypii	AGOS_AGR004W	NP_986669.1
Magnaporthe oryzae (rice blast fungus)	MGG_08590	XP_003716118.1
Oryza sativa (rice)	Os03g0349000	NP_001050106.1
	NUP93	XP_002802537.1
Mus musculus (house mouse)	Nup93	NP_765998.1
Rattus norvegicus (Norway rat)	Nup93	NP_001011925.1
Drosophila melanogaster (fruit fly)	CG7262	NP_650404.1
Caenorhabditis elegans (roundworm)	npp-13	NP_740802.1
Saccharomyces cerevisiae (baker's yeast)	NIC96	NP_116657.1
Xenopus tropicalis (tropical clawed frog)	nup93	NP_998879.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Murakami Y, Naitou M, Hagiwara H, Shibata T, Ozawa M, Sasanuma S, Sasanuma M, Tsuchiya Y, Soeda E, Yokoyama K
Nature genetics10(3)261-8(1995 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The crystal structure of Nic96 without its N domain has of 3 domains & is a straight molecule that--although almost entirely helical--exhibits big deviations from the predicted alpha-solenoid fold. The missing N domain projects midway from the molecule.
Title: Structural basis of the nic96 subcomplex organization in the nuclear pore channel.

nucleoporin Nic96 has a novel, intricate helical domain architecture as shown by crystallographic analysis
Title: Crystal structure of nucleoporin Nic96 reveals a novel, intricate helical domain architecture.

The following NIC96 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NIC96 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00238	NM_001179967.1 Latest version!	Saccharomyces cerevisiae S288C linker nucleoporin NIC96 (NIC96), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NIC96 ( NM_001179967.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_116657.1 Saccharomyces cerevisiae S288C linker nucleoporin NIC96 (NIC96), partial mRNA.

Price & Availability↑

CloneID	OSi00238
Clone ID Related Accession (Same CDS sequence)	NM_001179967.1
Accession Version	NM_001179967.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2520bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	linker nucleoporin NIC96
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001138). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGCTGGAGA CGTTGCGCGG AAATAAGCTG CATTCTGGCA CCTCAAAAGG CGCCAACAAA 
AAGTTGAATG AACTCTTGGA ATCGTCCGAC AATCTACCAT CAGCATCCTC TGAATTAGGT 
TCAATTCAAG TAAGCATTAA TGAATTGAGA AGACGTGTTT TTCAATTGAG AAGCAAGAAC 
AAAGCTAGCA AGGACTATAC TAAAGCCCAC TATTTATTGG CTAACAGTGG CTTAAGCTTT 
GAAGATGTTG ATGCTTTCAT CAAAGACTTG CAGACGAATC AATTTTTGGA ACCAAATCCA 
CCAAAAATAA TTGAAAGTGA GGAATTAGAA TTCTATATAA GAACTAAAAA GGAAGAAAAC 
ATTTTGATGT CTATCGAACA GCTTTTAAAT GGCGCTACAA AAGATTTTGA TAATTTTATT 
AATCATAACT TAAATCTTGA TTGGGCTCAG CATAAAAATG AAGTCATGAA AAATTTTGGC 
ATCCTTATAC AAGATAAGAA AACTGTAGAT CACAAAAAAT CCATCAGTTC TCTAGACCCT 
AAGCTTCCAA GCTGGGGTAA TAAGGGCAAT AACATTTTAA ATAGCAACGA ATCCAGATTG 
AATGTCAACG AAAATAATAT CCTTAGGGAA AAGTTTGAAA ATTATGCCAG AATTGTGTTT 
CAATTTAACA ATAGTAGACA AGCAAATGGA AATTTTGATA TAGCCAACGA ATTCATCTCA 
ATATTATCTT CTGCGAACGG AACAAGAAAC GCTCAATTGC TAGAGAGCTG GAAGATTCTC 
GAATCGATGA AAAGTAAGGA TATTAATATT GTTGAAGTCG GGAAACAGTA CCTGGAGCAA 
CAGTTTTTAC AATATACCGA CAATTTATAT AAGAAAAACA TGAATGAAGG ATTGGCCACA 
AATGTCAATA AAATCAAGTC ATTCATTGAT ACGAAGTTGA AGAAAGCAGA CAAATCATGG 
AAGATTTCTA ATTTAACTGT TATCAATGGT GTTCCAATAT GGGCCTTAAT ATTTTATCTT 
TTAAGAGCAG GTCTAATCAA GGAAGCATTG CAAGTTCTAG TGGAGAATAA GGCGAATATC 
AAAAAAGTAG AACAATCATT CCTTACTTAT TTCAAAGCAT ATGCTTCTTC AAAGGACCAC 
GGCCTACCAG TGGAATATTC CACCAAGCTT CATACAGAAT ACAACCAACA CATAAAAAGT 
TCACTAGATG GTGATCCTTA TCGTCTAGCT GTCTACAAGT TGATCGGAAG ATGCGACTTG 
AGTAGAAAGA ACATTCCTGC AGTTACTTTA AGCATTGAAG ATTGGTTGTG GATGCATCTC 
ATGTTGATCA AAGAAAAGGA CGCGGAGAAT GATCCTGTTT ATGAAAGATA TTCCTTAGAA 
GACTTCCAGA ATATAATAAT ATCGTATGGA CCATCTCGCT TCTCAAACTA TTACTTACAG 
ACCCTTTTGT TAAGTGGGCT CTATGGATTG GCTATTGACT ATACTTATAC ATTTAGTGAA 
ATGGATGCGG TGCATTTGGC AATAGGGCTA GCAAGCTTGA AGCTATTTAA GATTGATAGC 
TCAACGCGCT TGACCAAAAA ACCCAAAAGG GATATCAGGT TCGCAAACAT ACTAGCTAAT 
TATACCAAGT CCTTTAGGTA TTCAGATCCT AGAGTTGCTG TCGAATATTT GGTTTTAATA 
ACTCTAAATG AAGGACCTAC CGATGTCGAG TTATGCCATG AGGCGTTGAG GGAGCTAGTA 
TTGGAAACTA AGGAATTTAC TGTTCTTTTG GGTAAAATTG GACGCGATGG AGCTAGAATT 
CCGGGGGTGA TCGAAGAAAG ACAACCTTTA TTACACGTTC GTGATGAAAA GGAGTTTTTG 
CATACAATTA CAGAACAAGC AGCAAGAAGG GCAGACGAAG ACGGCAGAAT ATATGATAGT 
ATATTACTGT ACCAGCTTGC TGAAGAATAT GACATAGTTA TTACCCTCGT CAATAGTTTA 
TTAAGTGATA CACTTAGTGC GTCAGATTTA GATCAACCAT TGGTTGGACC TGATGACAAT 
AGCGAGACGA ATCCTGTCTT GTTGGCTAGG AGGATGGCGT CCATATATTT TGATAATGCT 
GGTATATCGA GACAGATTCA TGTTAAAAAC AAAGAAATTT GTATGCTGCT CTTAAATATC 
TCCTCTATCA GGGAGCTATA TTTCAATAAA CAATGGCAAG AAACACTATC CCAAATGGAG 
TTATTGGATT TACTTCCTTT CTCAGACGAG CTATCTGCTA GAAAGAAAGC ACAAGATTTC 
TCTAATTTAG ATGACAACAT TGTGAAAAAT ATTCCAAATT TGTTGATTAT CACTCTAAGC 
TGTATATCGA ACATGATTCA TATCTTGAAC GAAAGTAAGT ATCAATCGTC CACAAAAGGT 
CAACAAATTG ATTCCTTAAA AAATGTTGCG AGACAATGTA TGATTTATGC TGGTATGATT 
CAATACAGAA TGCCAAGGGA AACGTACAGC ACTTTAATTA ATATAGACGT CTCTCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116657.1
CDS	1..2520
Translation	MLETLRGNKLHSGTSKGANKKLNELLESSDNLPSASSELGSIQVSINELRRRVFQLRSKNKASKDYTKAHYLLANSGLSFEDVDAFIKDLQTNQFLEPNPPKIIESEELEFYIRTKKEENILMSIEQLLNGATKDFDNFINHNLNLDWAQHKNEVMKNFGILIQDKKTVDHKKSISSLDPKLPSWGNKGNNILNSNESRLNVNENNILREKFENYARIVFQFNNSRQANGNFDIANEFISILSSANGTRNAQLLESWKILESMKSKDINIVEVGKQYLEQQFLQYTDNLYKKNMNEGLATNVNKIKSFIDTKLKKADKSWKISNLTVINGVPIWALIFYLLRAGLIKEALQVLVENKANIKKVEQSFLTYFKAYASSKDHGLPVEYSTKLHTEYNQHIKSSLDGDPYRLAVYKLIGRCDLSRKNIPAVTLSIEDWLWMHLMLIKEKDAENDPVYERYSLEDFQNIIISYGPSRFSNYYLQTLLLSGLYGLAIDYTYTFSEMDAVHLAIGLASLKLFKIDSSTRLTKKPKRDIRFANILANYTKSFRYSDPRVAVEYLVLITLNEGPTDVELCHEALRELVLETKEFTVLLGKIGRDGARIPGVIEERQPLLHVRDEKEFLHTITEQAARRADEDGRIYDSILLYQLAEEYDIVITLVNSLLSDTLSASDLDQPLVGPDDNSETNPVLLARRMASIYFDNAGISRQIHVKNKEICMLLLNISSIRELYFNKQWQETLSQMELLDLLPFSDELSARKKAQDFSNLDDNIVKNIPNLLIITLSCISNMIHILNESKYQSSTKGQQIDSLKNVARQCMIYAGMIQYRMPRETYSTLINIDVSL

Target ORF information:

RefSeq Version	NM_001179967.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C linker nucleoporin NIC96 (NIC96), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179967.1

ORF Insert Sequence:

ATGCTGGAGA CGTTGCGCGG AAATAAGCTG CATTCTGGCA CCTCAAAAGG CGCCAACAAA 
AAGTTGAATG AACTCTTGGA ATCGTCCGAC AATCTACCAT CAGCATCCTC TGAATTAGGT 
TCAATTCAAG TAAGCATTAA TGAATTGAGA AGACGTGTTT TTCAATTGAG AAGCAAGAAC 
AAAGCTAGCA AGGACTATAC TAAAGCCCAC TATTTATTGG CTAACAGTGG CTTAAGCTTT 
GAAGATGTTG ATGCTTTCAT CAAAGACTTG CAGACGAATC AATTTTTGGA ACCAAATCCA 
CCAAAAATAA TTGAAAGTGA GGAATTAGAA TTCTATATAA GAACTAAAAA GGAAGAAAAC 
ATTTTGATGT CTATCGAACA GCTTTTAAAT GGCGCTACAA AAGATTTTGA TAATTTTATT 
AATCATAACT TAAATCTTGA TTGGGCTCAG CATAAAAATG AAGTCATGAA AAATTTTGGC 
ATCCTTATAC AAGATAAGAA AACTGTAGAT CACAAAAAAT CCATCAGTTC TCTAGACCCT 
AAGCTTCCAA GCTGGGGTAA TAAGGGCAAT AACATTTTAA ATAGCAACGA ATCCAGATTG 
AATGTCAACG AAAATAATAT CCTTAGGGAA AAGTTTGAAA ATTATGCCAG AATTGTGTTT 
CAATTTAACA ATAGTAGACA AGCAAATGGA AATTTTGATA TAGCCAACGA ATTCATCTCA 
ATATTATCTT CTGCGAACGG AACAAGAAAC GCTCAATTGC TAGAGAGCTG GAAGATTCTC 
GAATCGATGA AAAGTAAGGA TATTAATATT GTTGAAGTCG GGAAACAGTA CCTGGAGCAA 
CAGTTTTTAC AATATACCGA CAATTTATAT AAGAAAAACA TGAATGAAGG ATTGGCCACA 
AATGTCAATA AAATCAAGTC ATTCATTGAT ACGAAGTTGA AGAAAGCAGA CAAATCATGG 
AAGATTTCTA ATTTAACTGT TATCAATGGT GTTCCAATAT GGGCCTTAAT ATTTTATCTT 
TTAAGAGCAG GTCTAATCAA GGAAGCATTG CAAGTTCTAG TGGAGAATAA GGCGAATATC 
AAAAAAGTAG AACAATCATT CCTTACTTAT TTCAAAGCAT ATGCTTCTTC AAAGGACCAC 
GGCCTACCAG TGGAATATTC CACCAAGCTT CATACAGAAT ACAACCAACA CATAAAAAGT 
TCACTAGATG GTGATCCTTA TCGTCTAGCT GTCTACAAGT TGATCGGAAG ATGCGACTTG 
AGTAGAAAGA ACATTCCTGC AGTTACTTTA AGCATTGAAG ATTGGTTGTG GATGCATCTC 
ATGTTGATCA AAGAAAAGGA CGCGGAGAAT GATCCTGTTT ATGAAAGATA TTCCTTAGAA 
GACTTCCAGA ATATAATAAT ATCGTATGGA CCATCTCGCT TCTCAAACTA TTACTTACAG 
ACCCTTTTGT TAAGTGGGCT CTATGGATTG GCTATTGACT ATACTTATAC ATTTAGTGAA 
ATGGATGCGG TGCATTTGGC AATAGGGCTA GCAAGCTTGA AGCTATTTAA GATTGATAGC 
TCAACGCGCT TGACCAAAAA ACCCAAAAGG GATATCAGGT TCGCAAACAT ACTAGCTAAT 
TATACCAAGT CCTTTAGGTA TTCAGATCCT AGAGTTGCTG TCGAATATTT GGTTTTAATA 
ACTCTAAATG AAGGACCTAC CGATGTCGAG TTATGCCATG AGGCGTTGAG GGAGCTAGTA 
TTGGAAACTA AGGAATTTAC TGTTCTTTTG GGTAAAATTG GACGCGATGG AGCTAGAATT 
CCGGGGGTGA TCGAAGAAAG ACAACCTTTA TTACACGTTC GTGATGAAAA GGAGTTTTTG 
CATACAATTA CAGAACAAGC AGCAAGAAGG GCAGACGAAG ACGGCAGAAT ATATGATAGT 
ATATTACTGT ACCAGCTTGC TGAAGAATAT GACATAGTTA TTACCCTCGT CAATAGTTTA 
TTAAGTGATA CACTTAGTGC GTCAGATTTA GATCAACCAT TGGTTGGACC TGATGACAAT 
AGCGAGACGA ATCCTGTCTT GTTGGCTAGG AGGATGGCGT CCATATATTT TGATAATGCT 
GGTATATCGA GACAGATTCA TGTTAAAAAC AAAGAAATTT GTATGCTGCT CTTAAATATC 
TCCTCTATCA GGGAGCTATA TTTCAATAAA CAATGGCAAG AAACACTATC CCAAATGGAG 
TTATTGGATT TACTTCCTTT CTCAGACGAG CTATCTGCTA GAAAGAAAGC ACAAGATTTC 
TCTAATTTAG ATGACAACAT TGTGAAAAAT ATTCCAAATT TGTTGATTAT CACTCTAAGC 
TGTATATCGA ACATGATTCA TATCTTGAAC GAAAGTAAGT ATCAATCGTC CACAAAAGGT 
CAACAAATTG ATTCCTTAAA AAATGTTGCG AGACAATGTA TGATTTATGC TGGTATGATT 
CAATACAGAA TGCCAAGGGA AACGTACAGC ACTTTAATTA ATATAGACGT CTCTCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Nature genetics10(3)261-8(1995 Jul)
Murakami Y,Naitou M,Hagiwara H,Shibata T,Ozawa M,Sasanuma S,Sasanuma M,Tsuchiya Y,Soeda E,Yokoyama K

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NIC96 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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