MSH4 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	MSH4
Entrez Gene ID	850545
Full Name	MutS family protein MSH4
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179963.1	NP_116652.1	MutS family protein MSH4

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MSH4	XP_524742.2
Kluyveromyces lactis	KLLA0F11022g	XP_455582.1
Canis lupus familiaris (dog)	MSH4	XP_005622187.1
Danio rerio (zebrafish)	msh4	XP_688406.1
Caenorhabditis elegans (roundworm)	him-14	NP_495451.1
Magnaporthe oryzae (rice blast fungus)	MGG_08738	XP_003719247.1
Arabidopsis thaliana (thale cress)	MSH4	NP_193469.2
Homo sapiens (human)	MSH4	NP_002431.2
	MSH4	XP_001101368.1
Gallus gallus (chicken)	MSH4	XP_004936961.1
Mus musculus (house mouse)	Msh4	NP_114076.1
Rattus norvegicus (Norway rat)	Msh4	NP_001099947.2
Saccharomyces cerevisiae (baker's yeast)	MSH4	NP_116652.1
Xenopus tropicalis (tropical clawed frog)	msh4	XP_002936134.2
Bos taurus (cattle)	MSH4	NP_001193184.1
Oryza sativa (rice)	Os07g0486000	NP_001175201.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Murakami Y, Naitou M, Hagiwara H, Shibata T, Ozawa M, Sasanuma S, Sasanuma M, Tsuchiya Y, Soeda E, Yokoyama K
Nature genetics10(3)261-8(1995 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The excess crossovers observed in synaptonemal complex (SC), central element-deficient mutants are Msh4, Zip1, and Zip4 dependent, clearly indicating the existence of two classes of SC proteins-a class with procrossover function(s) that are also necessary for SC assembly and a class that is not required for crossover formation but essential for SC assembly
Title: Synaptonemal Complex Proteins of Budding Yeast Define Reciprocal Roles in MutS?-Mediated Crossover Formation.

a structural rationale for the meiotic defects observed in the msh4/5 point mutations. The mutations are likely to affect stability of the Msh4/5 proteins and/or interactions with DNA.
Title: Structural insights into Saccharomyces cerevisiae Msh4-Msh5 complex function using homology modeling.

Msh4 and msh5 mutants displayed reduced crossing over in meiosis but maintained wild-type spore viability.
Title: Genetic analysis of baker's yeast Msh4-Msh5 reveals a threshold crossover level for meiotic viability.

analysis of temperature-dependent modulation of chromosome segregation in msh4 mutants of budding yeast
Title: Temperature-dependent modulation of chromosome segregation in msh4 mutants of budding yeast.

The following MSH4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MSH4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00232	NM_001179963.1 Latest version!	Saccharomyces cerevisiae S288C MutS family protein MSH4 (MSH4), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MSH4 ( NM_001179963.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_116652.1 Saccharomyces cerevisiae S288C MutS family protein MSH4 (MSH4), partial mRNA.

Price & Availability↑

CloneID	OSi00232
Clone ID Related Accession (Same CDS sequence)	NM_001179963.1
Accession Version	NM_001179963.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2637bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	MutS family protein MSH4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001138). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAGTGAAT CTAATCTATC TAGTTTTATC AGTACAAATT ATTTCAATTT AAGGTCGGCG 
GCTAATTCTT CAAACTCTAT TTCGAAGCCA AGTACGAAAA AGAGTATCAG GAACCAAAAA 
TCGCCAACTA ACATAAGTAG TTGGGCTCTC AAAAAGAAAA CGCTACAAAT AGCAGAGACA 
ACATGGGAAA ACAATGAAAA AGATTCAACA CATAGCCATT ACTTAATGAC TGGATCAATG 
GCCTCTAGGA CCGCAACCTC CTTAAGTCGA TACAGCACCA ACGCTTCACT CCTCGGTCCT 
AGTATTGACT GTGTTCTTTG TTGCATATAC GAAGTGCCAA GAGACATAAG TACGCGGATT 
GGGTTATGTA TTATCAACTG TAACACGGGA CAGATGTATC TTTCAGATTT CATGGATTCA 
CAAATTTACA TTCGTGTAGT TCATAAACTC CAAATTTACC AACCTACGGA AATACTGATT 
CCAAGCAGTT CTCTAGCTCC AACAGTATCA AAATTAGCGA CAATGATAAA ATTTAATGTG 
GCCGAGACAG TTAAAATTGA AGAGGGTTCA CGAAAGTGCT TCAATAGCCA AGATGGACTG 
GCGGCGATTA CAAAGTATTT AATGGATGAT ACAAAAAAGG ACTTGAAAAT CGAGGAGATT 
ATAGATAAAA CGTTTGCTCT TTGTGCTGCA TCTGCTGCGA TAAGCTACAT GGAAGAGATT 
ATATCCAAAA GCAGCCGAAA TTTGAATGCG TTTCGAAAAC TACGTATACA GTTTGAAGGA 
ACAGAAAATA CAATGCTGAT TGATTCTAAA ACCGTCAGAG GTTTAGAACT TGTAGAAAAT 
AAATTGGATA AGAATGGCAT ATCACTATGG AAGTTCTTGG ATACCACGTC CACTAAAATG 
GGACAAAGAT CACTTCGGAA CAGTATACTG CAGCCATTAA CTGATAGGGG CAGTATCGAA 
ATGAGACTAG AGGCCCTAGA AGAATTGAAA GCCAATGATG ACCTTCTACA AAAATTAAGG 
CTAGAAATGA AATCATTGCC TGATCTAGAT AAGTTATTTT CAAGGTTATT ATGCATTAAT 
CATTCTGCAA TAAAACCTGA CCAAAGAATT AATTACGTTT TGTTGCTAAA GGAAACTTTA 
CAAAGCGTGA AGTCATTGAA AGACGCACTT AATGATCAGT TAATTCAAAG CCGTTTAATT 
AGCGAGACCA AAAAAATATT CAATAATGAC GCAATTATGG AAATTGAAAA ATTAATTAAT 
AGTTGTATAA ATGAAGACTG TGTCTGGGCA TCCTCAGCTA TACAACTACT TAACCAACGT 
TCATATGCTG TGAAAAGTGA TTCCAATGGG CTTTTGGATG TTTCTAGACA AATCTACAAA 
GAGGTAAAAG AGGAATTTTT TCGTGAGGTA GAAGATCTTA CCGCTAAAAA CAAAATCAAT 
TTAGATCATA ATTACGATTC TGCACGAGGC TTTTATCTCA GAATAAAAAG ACAGGAATTT 
ACCGATGACG TCGCTACACT TCCAGACGTA TTCATTTCAA GAACAATAAA AAAAAATTAT 
ATTGAATGTA CAACCCTAAA TATAATAAAA AAAAATGCGA GGTTGAAGGA AGTTATGGAA 
GAGATTTTAC TTTTAAGTGA AGAGACAGTT GATGAACTGC TCGATAAAAT TGCAACCCAT 
ATTTCTGAAC TATTTATGAT TGCAGAAGCA GTGGCTATCC TGGATTTGGT TTGTTCATTC 
ACATATAATT TGAAAGAAAA TAATTACACA ATACCGATTT TTACAAACAA TCTACTAATT 
CGAGATTCAA GACACCCACT ACTTGAAAAG GTACTAAAAA ATTTTGTCCC TAACACCATA 
TCAAGTACAA AACATAGCTC CAGCTTACAA ATCATAACCG GATGCAACAT GAGTGGAAAA 
TCTGTTTATT TAAAACAAGT TGCCCTTATT TGCATAATGG CCCAAATGGG ATCAGGTATA 
CCGGCACTTT ATGGGTCTTT TCCTGTCTTT AAACGTCTAC ATGCAAGAGT CTGCAATGAC 
AGTATGGAAT TAACCTCATC AAATTTTGGT TTTGAAATGA AAGAAATGGC TTACTTTTTA 
GATGACATAA ATACAGAAAC ACTGTTAATT TTAGATGAAC TTGGTCGTGG TTCAAGCATC 
GCTGATGGGT TTTGTGTGTC TTTGGCAGTA ACTGAACATT TACTTCGAAC GGAGGCAACG 
GTATTTCTTT CTACACACTT CCAGGACATT CCTAAAATAA TGTCTAAGAA GCCAGCAGTT 
TCCCATCTTC ATATGGATGC AGTCCTTCTC AATGACAATT CTGTCAAAAT GAATTATCAG 
TTGACCCAGA AATCTGTCGC AATAGAGAAT AGTGGAATAA GGGTGGTAAA AAAAATATTC 
AACCCGGATA TTATTGCAGA AGCGTACAAT ATCCATTCGC TTTTGAAAAT TGCAAAAGCA 
AGGACTGAAA ACGAAGATTC AAATGGCGTT GTTGATCAAA AGACCATAAA CCAAATGAAG 
AGAATACACA ATCTTGTAGC GATATTGAAA GAATGTGCAG GAAATGAAAA AGAGCCCTTG 
ACTTTAGGGA AATTAAAAGA AATAAACTCC GACTTCATCG AAAATTTTGA AGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116652.1
CDS	1..2637
Translation	MSESNLSSFISTNYFNLRSAANSSNSISKPSTKKSIRNQKSPTNISSWALKKKTLQIAETTWENNEKDSTHSHYLMTGSMASRTATSLSRYSTNASLLGPSIDCVLCCIYEVPRDISTRIGLCIINCNTGQMYLSDFMDSQIYIRVVHKLQIYQPTEILIPSSSLAPTVSKLATMIKFNVAETVKIEEGSRKCFNSQDGLAAITKYLMDDTKKDLKIEEIIDKTFALCAASAAISYMEEIISKSSRNLNAFRKLRIQFEGTENTMLIDSKTVRGLELVENKLDKNGISLWKFLDTTSTKMGQRSLRNSILQPLTDRGSIEMRLEALEELKANDDLLQKLRLEMKSLPDLDKLFSRLLCINHSAIKPDQRINYVLLLKETLQSVKSLKDALNDQLIQSRLISETKKIFNNDAIMEIEKLINSCINEDCVWASSAIQLLNQRSYAVKSDSNGLLDVSRQIYKEVKEEFFREVEDLTAKNKINLDHNYDSARGFYLRIKRQEFTDDVATLPDVFISRTIKKNYIECTTLNIIKKNARLKEVMEEILLLSEETVDELLDKIATHISELFMIAEAVAILDLVCSFTYNLKENNYTIPIFTNNLLIRDSRHPLLEKVLKNFVPNTISSTKHSSSLQIITGCNMSGKSVYLKQVALICIMAQMGSGIPALYGSFPVFKRLHARVCNDSMELTSSNFGFEMKEMAYFLDDINTETLLILDELGRGSSIADGFCVSLAVTEHLLRTEATVFLSTHFQDIPKIMSKKPAVSHLHMDAVLLNDNSVKMNYQLTQKSVAIENSGIRVVKKIFNPDIIAEAYNIHSLLKIAKARTENEDSNGVVDQKTINQMKRIHNLVAILKECAGNEKEPLTLGKLKEINSDFIENFEE

Target ORF information:

RefSeq Version	NM_001179963.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C MutS family protein MSH4 (MSH4), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179963.1

ORF Insert Sequence:

ATGAGTGAAT CTAATCTATC TAGTTTTATC AGTACAAATT ATTTCAATTT AAGGTCGGCG 
GCTAATTCTT CAAACTCTAT TTCGAAGCCA AGTACGAAAA AGAGTATCAG GAACCAAAAA 
TCGCCAACTA ACATAAGTAG TTGGGCTCTC AAAAAGAAAA CGCTACAAAT AGCAGAGACA 
ACATGGGAAA ACAATGAAAA AGATTCAACA CATAGCCATT ACTTAATGAC TGGATCAATG 
GCCTCTAGGA CCGCAACCTC CTTAAGTCGA TACAGCACCA ACGCTTCACT CCTCGGTCCT 
AGTATTGACT GTGTTCTTTG TTGCATATAC GAAGTGCCAA GAGACATAAG TACGCGGATT 
GGGTTATGTA TTATCAACTG TAACACGGGA CAGATGTATC TTTCAGATTT CATGGATTCA 
CAAATTTACA TTCGTGTAGT TCATAAACTC CAAATTTACC AACCTACGGA AATACTGATT 
CCAAGCAGTT CTCTAGCTCC AACAGTATCA AAATTAGCGA CAATGATAAA ATTTAATGTG 
GCCGAGACAG TTAAAATTGA AGAGGGTTCA CGAAAGTGCT TCAATAGCCA AGATGGACTG 
GCGGCGATTA CAAAGTATTT AATGGATGAT ACAAAAAAGG ACTTGAAAAT CGAGGAGATT 
ATAGATAAAA CGTTTGCTCT TTGTGCTGCA TCTGCTGCGA TAAGCTACAT GGAAGAGATT 
ATATCCAAAA GCAGCCGAAA TTTGAATGCG TTTCGAAAAC TACGTATACA GTTTGAAGGA 
ACAGAAAATA CAATGCTGAT TGATTCTAAA ACCGTCAGAG GTTTAGAACT TGTAGAAAAT 
AAATTGGATA AGAATGGCAT ATCACTATGG AAGTTCTTGG ATACCACGTC CACTAAAATG 
GGACAAAGAT CACTTCGGAA CAGTATACTG CAGCCATTAA CTGATAGGGG CAGTATCGAA 
ATGAGACTAG AGGCCCTAGA AGAATTGAAA GCCAATGATG ACCTTCTACA AAAATTAAGG 
CTAGAAATGA AATCATTGCC TGATCTAGAT AAGTTATTTT CAAGGTTATT ATGCATTAAT 
CATTCTGCAA TAAAACCTGA CCAAAGAATT AATTACGTTT TGTTGCTAAA GGAAACTTTA 
CAAAGCGTGA AGTCATTGAA AGACGCACTT AATGATCAGT TAATTCAAAG CCGTTTAATT 
AGCGAGACCA AAAAAATATT CAATAATGAC GCAATTATGG AAATTGAAAA ATTAATTAAT 
AGTTGTATAA ATGAAGACTG TGTCTGGGCA TCCTCAGCTA TACAACTACT TAACCAACGT 
TCATATGCTG TGAAAAGTGA TTCCAATGGG CTTTTGGATG TTTCTAGACA AATCTACAAA 
GAGGTAAAAG AGGAATTTTT TCGTGAGGTA GAAGATCTTA CCGCTAAAAA CAAAATCAAT 
TTAGATCATA ATTACGATTC TGCACGAGGC TTTTATCTCA GAATAAAAAG ACAGGAATTT 
ACCGATGACG TCGCTACACT TCCAGACGTA TTCATTTCAA GAACAATAAA AAAAAATTAT 
ATTGAATGTA CAACCCTAAA TATAATAAAA AAAAATGCGA GGTTGAAGGA AGTTATGGAA 
GAGATTTTAC TTTTAAGTGA AGAGACAGTT GATGAACTGC TCGATAAAAT TGCAACCCAT 
ATTTCTGAAC TATTTATGAT TGCAGAAGCA GTGGCTATCC TGGATTTGGT TTGTTCATTC 
ACATATAATT TGAAAGAAAA TAATTACACA ATACCGATTT TTACAAACAA TCTACTAATT 
CGAGATTCAA GACACCCACT ACTTGAAAAG GTACTAAAAA ATTTTGTCCC TAACACCATA 
TCAAGTACAA AACATAGCTC CAGCTTACAA ATCATAACCG GATGCAACAT GAGTGGAAAA 
TCTGTTTATT TAAAACAAGT TGCCCTTATT TGCATAATGG CCCAAATGGG ATCAGGTATA 
CCGGCACTTT ATGGGTCTTT TCCTGTCTTT AAACGTCTAC ATGCAAGAGT CTGCAATGAC 
AGTATGGAAT TAACCTCATC AAATTTTGGT TTTGAAATGA AAGAAATGGC TTACTTTTTA 
GATGACATAA ATACAGAAAC ACTGTTAATT TTAGATGAAC TTGGTCGTGG TTCAAGCATC 
GCTGATGGGT TTTGTGTGTC TTTGGCAGTA ACTGAACATT TACTTCGAAC GGAGGCAACG 
GTATTTCTTT CTACACACTT CCAGGACATT CCTAAAATAA TGTCTAAGAA GCCAGCAGTT 
TCCCATCTTC ATATGGATGC AGTCCTTCTC AATGACAATT CTGTCAAAAT GAATTATCAG 
TTGACCCAGA AATCTGTCGC AATAGAGAAT AGTGGAATAA GGGTGGTAAA AAAAATATTC 
AACCCGGATA TTATTGCAGA AGCGTACAAT ATCCATTCGC TTTTGAAAAT TGCAAAAGCA 
AGGACTGAAA ACGAAGATTC AAATGGCGTT GTTGATCAAA AGACCATAAA CCAAATGAAG 
AGAATACACA ATCTTGTAGC GATATTGAAA GAATGTGCAG GAAATGAAAA AGAGCCCTTG 
ACTTTAGGGA AATTAAAAGA AATAAACTCC GACTTCATCG AAAATTTTGA AGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Nature genetics10(3)261-8(1995 Jul)
Murakami Y,Naitou M,Hagiwara H,Shibata T,Ozawa M,Sasanuma S,Sasanuma M,Tsuchiya Y,Soeda E,Yokoyama K

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MSH4 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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