PHO87 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	PHO87
Entrez Gene ID	850403
Full Name	SPX domain-containing inorganic phosphate transporter
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178751.1	NP_009966.2	SPX domain-containing inorganic phosphate transporter

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	PHO90	NP_012337.1
Eremothecium gossypii	AGOS_AFR628C	NP_986175.2
Saccharomyces cerevisiae (baker's yeast)	PHO87	NP_009966.2
Kluyveromyces lactis	KLLA0C03454g	XP_452350.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

The complete DNA sequence of yeast chromosome III.
Oliver SG, van der Aart QJ, Agostoni-Carbone ML, Aigle M, Alberghina L, Alexandraki D, Antoine G, Anwar R, Ballesta JP, Benit P
Nature357(6373)38-46(1992 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Pho87 and Pho90 represent non-redundant P(i) transporters, which are tuned by the integration of multiple nutrient signalling mechanisms in order to adjust P(i)-transport capacity to the general nutritional status of the environment.
Title: Differential roles for the low-affinity phosphate transporters Pho87 and Pho90 in Saccharomyces cerevisiae.

However, our results revealed that Ddi1p and its associated protein Rad23p are not involved in the decrease in Pho81p level under high-phosphate condition.
Title: Ddi1p and Rad23p play a cooperative role as negative regulators in the PHO pathway in Saccharomyces cerevisiae.

The following PHO87 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PHO87 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00113	NM_001178751.1 Latest version!	Saccharomyces cerevisiae S288C SPX domain-containing inorganic phosphate transporter (PHO87), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PHO87 ( NM_001178751.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_009966.2 Saccharomyces cerevisiae S288C SPX domain-containing inorganic phosphate transporter (PHO87), partial mRNA.

Price & Availability↑

CloneID	OSi00113
Clone ID Related Accession (Same CDS sequence)	NM_001178751.1
Accession Version	NM_001178751.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	SPX domain-containing inorganic phosphate transporter
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001135). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAGATTCT CACACTTTCT CAAATACAAC GCTGTCCCTG AATGGCAGAA TCATTACCTA 
GATTATAACG AATTGAAAAA TTTGATCTAC ACATTACAGA CAGATGAATT GAAACAAGAA 
ACGCCAACCG GTGACTTAAA CGATGACGCT GACTCTCAGA CTCCAGGTCC AATCGCTGAT 
ATAGAAAGCA ACATAGCTGC AGGAGAACCA TCTTCATCGA AAAGAAGATT TACACATAAA 
CTCAAGCGTA AGCTCTTTGG TTCTAAAACA CCTTCAGGAA GCAAAAGGGG AGACTCCGAC 
GAAAAGGCCA TAGATGGGAA CAATATTAAC GAGGAAACAA TTGAGTTAGA CGAGTTATCT 
CCTCAAGGGA AAACCACCTC TTTCAATAAG AATTTTATAC GTAAGAAATT CTTTGAATCA 
CGCAGCTCAT CTGTGAGTAG CGAGGGAAAG ACGCTCTTCA GTTCTTATGA TACATTCGTA 
ACTAACCTGA GCGACGAGAA ATTGAAAGTA GATGATTTCT ACAAAAGAAT GGAAGCTAAG 
TTCTATGAAA GATTTGACCA CTTGATTAAT GATTTGGAGA AGGAAGGCAT TGTAACAAGA 
TTGAATGAAA CTTTCAATCC TGAAATTCAA GCATTGCCTC CTTTAAGAGA AATTATTTCT 
GGTACATCAG AGACACATTC ATCTAATAAC CCATTTGAAA TACACTCTTC AAACATCGAC 
AGTGAATTGA GAAATAGGTT TGATTACAGC GAAGAAGAAA TGGATGAAGA TGATGACGTT 
GACGTGTTTG CTGACACTAC CGACAATACC GCCCTCTTGA ATTATTCGCA ATTTAACATT 
AAATCTCAGA AAAAGTCATT ATTAAAACAG ACAATAATAA ATCTTTACAT AGACCTTTGC 
CAGTTGAAAT CTTTTATCGA ATTGAACAGA ATGGGTTTCA GTAAAATTAC TAAGAAGTCT 
GATAAAGTAT TGCACATGAA CACTAGGCAA GAATTAATAG AAAGTGAAGA ATTTTTCAAA 
GACACCTACA TCTTCCAGCA TGAAACTTTA AGCAGTTTAA ACAGTAAAAT TGCACAACTT 
ATTGAATTTT ATGCTGTTCT CATGGGTCAG CCTGGGAACG TAGATTCATG CAAGCAAGAG 
TTAAAGTCGT ACCTGCACGA CCACATTGTT TGGGAAAGAA GCAACACATG GAAAGACATG 
TTGGGCCTCT CTTCGCAAAA TAACGATATA ATAACTATTG AAGATGAAGC TGAGAAACTT 
ATGCAAGAAA AGCTTCAAAT TGAATATTTC AAGTATCCAT TGCCTAAGCC AATTAATTTG 
AAGTTTACTA AAATTGAAAA TTTGGCAGTT CCTAAGCTAT TTTTTGGGAA AAGAGCAATG 
AAAATAGGCT TCATTATCAT TGTCACAGGT GTTTTGTTGG GTGTTAAAAC TTTCAATGAC 
CCTGTCGAAC ACCGGTGTAT GGCATTGGTA GAATGCTGTG CTTTCTTATG GGCTAGTGAA 
GCCATTCCAT TACACATCAC AGGTTTATTG GTTCCCCTTC TAACTGTCCT TTTTAGGGTA 
CTAAAAGACG ATGACGGTAA GGTAATGGGA GCAGCAGCTG CCTCTACAGA AATCTTAGGT 
ACAATGTGGT CGTCAACAAT TATGATTTTA TTAGCAGGTT TCACATTGGG TGAAGCCTTG 
TCGCAATATA ACGTTGCGAA AGTTTTGGCA TCGTGGTTAT TGGCCCTTGC AGGTACCAAG 
CCAAGAAATG TCCTTTTAAT GGCAATGAGT GTTGTATTCT TTCTTTCGAT GTGGATTTCC 
AACGTTGCCT CCCCAGTATT GACATATTCT CTATTAACAC CCTTACTAGA TCCGCTGGAC 
TACACTTCAC CGTTTGCTAA GGCATTAGTC ATGGGTGTTG CACTTTCGGC AGATATTGGT 
GGTATGGCTT CACCTATTTC TTCGCCACAG AATATCATCT CCATGCAGTA CTTAAAACCT 
TATGGAATCG GCTGGGGGCA ATTTTTTGCT GTCGCTCTGC CTACAGGTAT TCTATCGATG 
CTGTGCTCCT GGGCCTTGAT GATACTCACC TTTAAAATAG GCAAAACTAA ACTGGAAAAA 
TTTAAACCAA TAAGGACCAG ATTTACTATA AAGCAATATT TTATCATCAT TGTAACTATT 
GCTACTATTC TTCTATGGTG TGTAGAGTCA CAAATAGAAA GTGCTTTTGG ATCGTCCGGT 
GAAATTGCAG TAATACCGAT AGTCCTGTTT TTTGGTACAG GTCTACTATC AACAAAGGAT 
TTCAACACAT TCCCTTGGTC AATTGTTGTT CTTGCTATGG GTGGTATAGC CCTTGGTAAG 
GCAGTTTCAT CTTCAGGCTT GTTGGTAACT ATTGCAAGAG CATTACAAAA GAAAATTCAG 
AACGATGGTG TTTTTGCTAT CTTATGTATT TTCGGTATTT TAATGTTAGT TGTGGGCACT 
TTTGTCTCAC ATACTGTGTC AGCAATCATC ATTATTCCCT TGGTGCAAGA AGTTGGTGAC 
AAATTATCCG ATCCAAAGGC AGCTCCAATT CTTGTGTTCG GTTGCGCCTT GTTAGCCTCA 
TGCGGTATGG GGTTGGCTTC ATCTGGATTT CCAAACGTTA CTGCTATTTC TATGACCGAT 
AAAAAGGGTA ATAGATGGCT AACTGTAGGC GCTTTTATCT CCAGAGGTGT TCCTGCTTCG 
TTGTTAGCGT TTGTCTGCGT AATTACTCTC GGTTATGGTA TTAGTTCTTC CGTCTTAAAA 
GGTAGCACTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009966.2
CDS	1..2772
Translation	MRFSHFLKYNAVPEWQNHYLDYNELKNLIYTLQTDELKQETPTGDLNDDADSQTPGPIADIESNIAAGEPSSSKRRFTHKLKRKLFGSKTPSGSKRGDSDEKAIDGNNINEETIELDELSPQGKTTSFNKNFIRKKFFESRSSSVSSEGKTLFSSYDTFVTNLSDEKLKVDDFYKRMEAKFYERFDHLINDLEKEGIVTRLNETFNPEIQALPPLREIISGTSETHSSNNPFEIHSSNIDSELRNRFDYSEEEMDEDDDVDVFADTTDNTALLNYSQFNIKSQKKSLLKQTIINLYIDLCQLKSFIELNRMGFSKITKKSDKVLHMNTRQELIESEEFFKDTYIFQHETLSSLNSKIAQLIEFYAVLMGQPGNVDSCKQELKSYLHDHIVWERSNTWKDMLGLSSQNNDIITIEDEAEKLMQEKLQIEYFKYPLPKPINLKFTKIENLAVPKLFFGKRAMKIGFIIIVTGVLLGVKTFNDPVEHRCMALVECCAFLWASEAIPLHITGLLVPLLTVLFRVLKDDDGKVMGAAAASTEILGTMWSSTIMILLAGFTLGEALSQYNVAKVLASWLLALAGTKPRNVLLMAMSVVFFLSMWISNVASPVLTYSLLTPLLDPLDYTSPFAKALVMGVALSADIGGMASPISSPQNIISMQYLKPYGIGWGQFFAVALPTGILSMLCSWALMILTFKIGKTKLEKFKPIRTRFTIKQYFIIIVTIATILLWCVESQIESAFGSSGEIAVIPIVLFFGTGLLSTKDFNTFPWSIVVLAMGGIALGKAVSSSGLLVTIARALQKKIQNDGVFAILCIFGILMLVVGTFVSHTVSAIIIIPLVQEVGDKLSDPKAAPILVFGCALLASCGMGLASSGFPNVTAISMTDKKGNRWLTVGAFISRGVPASLLAFVCVITLGYGISSSVLKGST

Target ORF information:

RefSeq Version	NM_001178751.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C SPX domain-containing inorganic phosphate transporter (PHO87), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178751.1

ORF Insert Sequence:

ATGAGATTCT CACACTTTCT CAAATACAAC GCTGTCCCTG AATGGCAGAA TCATTACCTA 
GATTATAACG AATTGAAAAA TTTGATCTAC ACATTACAGA CAGATGAATT GAAACAAGAA 
ACGCCAACCG GTGACTTAAA CGATGACGCT GACTCTCAGA CTCCAGGTCC AATCGCTGAT 
ATAGAAAGCA ACATAGCTGC AGGAGAACCA TCTTCATCGA AAAGAAGATT TACACATAAA 
CTCAAGCGTA AGCTCTTTGG TTCTAAAACA CCTTCAGGAA GCAAAAGGGG AGACTCCGAC 
GAAAAGGCCA TAGATGGGAA CAATATTAAC GAGGAAACAA TTGAGTTAGA CGAGTTATCT 
CCTCAAGGGA AAACCACCTC TTTCAATAAG AATTTTATAC GTAAGAAATT CTTTGAATCA 
CGCAGCTCAT CTGTGAGTAG CGAGGGAAAG ACGCTCTTCA GTTCTTATGA TACATTCGTA 
ACTAACCTGA GCGACGAGAA ATTGAAAGTA GATGATTTCT ACAAAAGAAT GGAAGCTAAG 
TTCTATGAAA GATTTGACCA CTTGATTAAT GATTTGGAGA AGGAAGGCAT TGTAACAAGA 
TTGAATGAAA CTTTCAATCC TGAAATTCAA GCATTGCCTC CTTTAAGAGA AATTATTTCT 
GGTACATCAG AGACACATTC ATCTAATAAC CCATTTGAAA TACACTCTTC AAACATCGAC 
AGTGAATTGA GAAATAGGTT TGATTACAGC GAAGAAGAAA TGGATGAAGA TGATGACGTT 
GACGTGTTTG CTGACACTAC CGACAATACC GCCCTCTTGA ATTATTCGCA ATTTAACATT 
AAATCTCAGA AAAAGTCATT ATTAAAACAG ACAATAATAA ATCTTTACAT AGACCTTTGC 
CAGTTGAAAT CTTTTATCGA ATTGAACAGA ATGGGTTTCA GTAAAATTAC TAAGAAGTCT 
GATAAAGTAT TGCACATGAA CACTAGGCAA GAATTAATAG AAAGTGAAGA ATTTTTCAAA 
GACACCTACA TCTTCCAGCA TGAAACTTTA AGCAGTTTAA ACAGTAAAAT TGCACAACTT 
ATTGAATTTT ATGCTGTTCT CATGGGTCAG CCTGGGAACG TAGATTCATG CAAGCAAGAG 
TTAAAGTCGT ACCTGCACGA CCACATTGTT TGGGAAAGAA GCAACACATG GAAAGACATG 
TTGGGCCTCT CTTCGCAAAA TAACGATATA ATAACTATTG AAGATGAAGC TGAGAAACTT 
ATGCAAGAAA AGCTTCAAAT TGAATATTTC AAGTATCCAT TGCCTAAGCC AATTAATTTG 
AAGTTTACTA AAATTGAAAA TTTGGCAGTT CCTAAGCTAT TTTTTGGGAA AAGAGCAATG 
AAAATAGGCT TCATTATCAT TGTCACAGGT GTTTTGTTGG GTGTTAAAAC TTTCAATGAC 
CCTGTCGAAC ACCGGTGTAT GGCATTGGTA GAATGCTGTG CTTTCTTATG GGCTAGTGAA 
GCCATTCCAT TACACATCAC AGGTTTATTG GTTCCCCTTC TAACTGTCCT TTTTAGGGTA 
CTAAAAGACG ATGACGGTAA GGTAATGGGA GCAGCAGCTG CCTCTACAGA AATCTTAGGT 
ACAATGTGGT CGTCAACAAT TATGATTTTA TTAGCAGGTT TCACATTGGG TGAAGCCTTG 
TCGCAATATA ACGTTGCGAA AGTTTTGGCA TCGTGGTTAT TGGCCCTTGC AGGTACCAAG 
CCAAGAAATG TCCTTTTAAT GGCAATGAGT GTTGTATTCT TTCTTTCGAT GTGGATTTCC 
AACGTTGCCT CCCCAGTATT GACATATTCT CTATTAACAC CCTTACTAGA TCCGCTGGAC 
TACACTTCAC CGTTTGCTAA GGCATTAGTC ATGGGTGTTG CACTTTCGGC AGATATTGGT 
GGTATGGCTT CACCTATTTC TTCGCCACAG AATATCATCT CCATGCAGTA CTTAAAACCT 
TATGGAATCG GCTGGGGGCA ATTTTTTGCT GTCGCTCTGC CTACAGGTAT TCTATCGATG 
CTGTGCTCCT GGGCCTTGAT GATACTCACC TTTAAAATAG GCAAAACTAA ACTGGAAAAA 
TTTAAACCAA TAAGGACCAG ATTTACTATA AAGCAATATT TTATCATCAT TGTAACTATT 
GCTACTATTC TTCTATGGTG TGTAGAGTCA CAAATAGAAA GTGCTTTTGG ATCGTCCGGT 
GAAATTGCAG TAATACCGAT AGTCCTGTTT TTTGGTACAG GTCTACTATC AACAAAGGAT 
TTCAACACAT TCCCTTGGTC AATTGTTGTT CTTGCTATGG GTGGTATAGC CCTTGGTAAG 
GCAGTTTCAT CTTCAGGCTT GTTGGTAACT ATTGCAAGAG CATTACAAAA GAAAATTCAG 
AACGATGGTG TTTTTGCTAT CTTATGTATT TTCGGTATTT TAATGTTAGT TGTGGGCACT 
TTTGTCTCAC ATACTGTGTC AGCAATCATC ATTATTCCCT TGGTGCAAGA AGTTGGTGAC 
AAATTATCCG ATCCAAAGGC AGCTCCAATT CTTGTGTTCG GTTGCGCCTT GTTAGCCTCA 
TGCGGTATGG GGTTGGCTTC ATCTGGATTT CCAAACGTTA CTGCTATTTC TATGACCGAT 
AAAAAGGGTA ATAGATGGCT AACTGTAGGC GCTTTTATCT CCAGAGGTGT TCCTGCTTCG 
TTGTTAGCGT TTGTCTGCGT AATTACTCTC GGTTATGGTA TTAGTTCTTC CGTCTTAAAA 
GGTAGCACTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

The complete DNA sequence of yeast chromosome III.
Nature357(6373)38-46(1992 May)
Oliver SG,van der Aart QJ,Agostoni-Carbone ML,Aigle M,Alberghina L,Alexandraki D,Antoine G,Anwar R,Ballesta JP,Benit P

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PHO87 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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