ORF » Species Summary » Saccharomyces cerevisiae S288C » SPB1 cDNA ORF clone

SPB1 cDNA ORF clone, Saccharomyces cerevisiae S288C

  • Gene

  • Clones

  • gRNAs

The following SPB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi00033 NM_001178698.1
Latest version!		 Saccharomyces cerevisiae S288C 27S pre-rRNA (guanosine2922-2'-O)-methyltransferase (SPB1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    SPB1 ( NM_001178698.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_009877.1 Saccharomyces cerevisiae S288C 27S pre-rRNA (guanosine2922-2'-O)-methyltransferase (SPB1), partial mRNA.
    Price & Availability↑
    CloneID OSi00033
    Clone ID Related Accession (Same CDS sequence) NM_001178698.1
    Accession Version NM_001178698.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2526bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product 27S pre-rRNA (guanosine2922-2'-O)-methyltransferase
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001135). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGGTAAGA CACAAAAGAA GAATAGTAAG GGACGTTTAG ATAGATACTA TTATCTAGCA 
    AAGGAGAAAG GTTATCGTGC TCGTTCATCC TTTAAGATTA TTCAGATTAA TGAAAAGTAT 
    GGCCACTTCT TAGAAAAATC GAAGGTTGTT ATTGATCTGT GTGCTGCTCC TGGTTCATGG 
    TGTCAAGTTG CATCCAAACT CTGTCCTGTC AACTCCTTAA TTATTGGTGT TGATATTGTT 
    CCAATGAAGC CGATGCCCAA TGTTATAACT TTTCAAAGTG ATATTACAAC AGAAGATTGT 
    AGATCAAAAT TGAGGGGTTA TATGAAGACT TGGAAAGCTG ATACAGTGTT GCATGATGGT 
    GCTCCTAATG TTGGTTTGGG TTGGGTTCAG GATGCTTTCA CCCAATCTCA ATTAACCTTA 
    CAAGCTTTGA AGTTGGCCGT CGAAAATTTG GTGGTGAACG GTACTTTTGT TACTAAAATT 
    TTCAGATCCA AGGATTATAA TAAATTGATT TGGGTTTTCC AACAATTGTT TGAGAAAGTT 
    GAAGCCACAA AACCACCCGC ATCAAGAAAT GTTTCTGCAG AAATTTTTGT AGTGTGTAAA 
    GGTTTCAAGG CACCAAAGAG GCTGGACCCA AGATTACTGG ATCCAAAAGA AGTTTTTGAA 
    GAATTGCCAG ATGGGCAACA GAATATGGAG TCCAAGATTT ATAATCCCGA AAAAAAAGTT 
    AGAAAAAGAC AAGGTTATGA GGAAGGTGAT AATTTACTGT ATCATGAAAC CTCAATTTTG 
    GATTTTGTCA GGACGGAAGA CCCAATAAGC ATGCTTGGAG AAATGAATAA GTTTACAATT 
    GATGAGAATG ACCATGAATG GAAGATCTTG AAGAAATTGA AACAAACCAC AGACGAGTTC 
    CGTTCCTGTA TTGAAGATTT AAAAGTCTTG GGTAAAAAGG ATTTTAAAAT GATCCTAAGA 
    TGGAGAAAGA TTGCAAGGGA AATCTTGGGC ATCGAAGTTA AGGACGACGC TAAAACAGAA 
    ATTGAAGTAG TACCGTTAAC AGAAGAGGAG CAAATTGAAA AAGATTTGCA AGGTTTACAG 
    GAGAAGCAGC GTCTAAATGT CAAGCGCGAA AGAAGAAGGA AGAACGAAAT GAAGCAAAAG 
    GAACTACAGA GAATGCAAAT GAACATGATA ACCCCCACTG ATATTGGTAT TGAAGCCGCA 
    AGTTTGGGTA AAGAATCGTT GTTTAATTTG AAAACTGCAG AAAAGACTGG TATCTTGAAC 
    GACTTGGCGA AGGGTAAGAA AAGAATGATT TTTACAGACG ATGAATTAGC CAAAGATAAT 
    GATATTTACA TTGATGAAAA CATCATGATC AAAGATAAGG ATTCTGCCGC TGATGCGGAC 
    GATTTAGAAA GCGAGTTGAA TGCCATGTAT AGTGATTATA AAACTAGAAG GTCGGAAAGG 
    GATGCCAAGT TTAGAGCTAA GCAAGCACGT GGTGGCGATA ATGAGGAAGA ATGGACCGGT 
    TTTAATGAGG GAAGTCTAGA AAAGAAAGAA GAGGAAGGTA AAGATTATAT CGAAGACAAT 
    GACGATGAAG GTGTCGAAGG AGACTCCGAT GATGATGAAG CCATCACCAA TTTGATTAGC 
    AAATTGAAGG GACAAGAAGG TGATCACAAA TTGAGTAGTA AGGCACGTAT GATTTTCAAT 
    GATCCGATAT TTAATAACGT TGAACCTGAT TTGCCAGTAA ATACCGTCAA TGATGGCATA 
    ATGAGTTCCG AGTCTGTTGG TGATATTTCT AAATTAAATA AGAAAAGAAA ACATGAAGAA 
    ATGCACCAGA AACAAGATGA AGCAGATTCT TCGGATGAGA GTTCAAGTGA TGACTCCGAT 
    TTCGAAATTG TGGCCAACGA TAATGCATCA GAAGAATTCG ATTCTGATTA TGATTCAGAG 
    GAAGAAAAAA ATCAAACAAA GAAAGAAAAG CATTCCAGAG ACATTGATAT TGCTACTGTT 
    GAAGCCATGA CTTTGGCACA TCAGTTAGCA TTGGGTCAGA AAAACAAGCA TGATCTTGTT 
    GATGAAGGTT TCAATAGATA CACCTTCCGT GACACCGAAA ATTTGCCAGA TTGGTTTTTA 
    GAAGATGAAA AGGAACATTC AAAGATAAAT AAGCCGATTA CTAAGGAGGC AGCGATGGCA 
    ATTAAAGAAA AAATAAAGGC AATGAACGCC CGTCCTATTA AGAAGGTTGC TGAAGCTAAG 
    GCAAGAAAGA GGATGCGTGC CGTAGCTCGG TTAGAAAAGA TCAAGAAGAA GGCGGGCTTG 
    ATTAACGATG ATTCAGACAA GACAGAGAAG GACAAGGCTG AAGAAATTTC TAGATTGATG 
    CGTAAGGTTA CCAAAAAACC AAAGACCAAG CCAAAGGTTA CTTTGGTTGT TGCCTCAGGT 
    AGGAACAAAG GTTTGGCAGG TAGACCAAAG GGCGTTAAGG GTAAGTATAA GATGGTTGAT 
    GGTGTCATGA AGAATGAACA AAGAGCCCTA AGACGTATCG CAAAGAAGCA TCACAAGAAA 
    AAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_009877.1
    CDS1..2526
    Translation

    Target ORF information:

    RefSeq Version NM_001178698.1
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C 27S pre-rRNA (guanosine2922-2'-O)-methyltransferase (SPB1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001178698.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGGTAAGA CACAAAAGAA GAATAGTAAG GGACGTTTAG ATAGATACTA TTATCTAGCA 
    AAGGAGAAAG GTTATCGTGC TCGTTCATCC TTTAAGATTA TTCAGATTAA TGAAAAGTAT 
    GGCCACTTCT TAGAAAAATC GAAGGTTGTT ATTGATCTGT GTGCTGCTCC TGGTTCATGG 
    TGTCAAGTTG CATCCAAACT CTGTCCTGTC AACTCCTTAA TTATTGGTGT TGATATTGTT 
    CCAATGAAGC CGATGCCCAA TGTTATAACT TTTCAAAGTG ATATTACAAC AGAAGATTGT 
    AGATCAAAAT TGAGGGGTTA TATGAAGACT TGGAAAGCTG ATACAGTGTT GCATGATGGT 
    GCTCCTAATG TTGGTTTGGG TTGGGTTCAG GATGCTTTCA CCCAATCTCA ATTAACCTTA 
    CAAGCTTTGA AGTTGGCCGT CGAAAATTTG GTGGTGAACG GTACTTTTGT TACTAAAATT 
    TTCAGATCCA AGGATTATAA TAAATTGATT TGGGTTTTCC AACAATTGTT TGAGAAAGTT 
    GAAGCCACAA AACCACCCGC ATCAAGAAAT GTTTCTGCAG AAATTTTTGT AGTGTGTAAA 
    GGTTTCAAGG CACCAAAGAG GCTGGACCCA AGATTACTGG ATCCAAAAGA AGTTTTTGAA 
    GAATTGCCAG ATGGGCAACA GAATATGGAG TCCAAGATTT ATAATCCCGA AAAAAAAGTT 
    AGAAAAAGAC AAGGTTATGA GGAAGGTGAT AATTTACTGT ATCATGAAAC CTCAATTTTG 
    GATTTTGTCA GGACGGAAGA CCCAATAAGC ATGCTTGGAG AAATGAATAA GTTTACAATT 
    GATGAGAATG ACCATGAATG GAAGATCTTG AAGAAATTGA AACAAACCAC AGACGAGTTC 
    CGTTCCTGTA TTGAAGATTT AAAAGTCTTG GGTAAAAAGG ATTTTAAAAT GATCCTAAGA 
    TGGAGAAAGA TTGCAAGGGA AATCTTGGGC ATCGAAGTTA AGGACGACGC TAAAACAGAA 
    ATTGAAGTAG TACCGTTAAC AGAAGAGGAG CAAATTGAAA AAGATTTGCA AGGTTTACAG 
    GAGAAGCAGC GTCTAAATGT CAAGCGCGAA AGAAGAAGGA AGAACGAAAT GAAGCAAAAG 
    GAACTACAGA GAATGCAAAT GAACATGATA ACCCCCACTG ATATTGGTAT TGAAGCCGCA 
    AGTTTGGGTA AAGAATCGTT GTTTAATTTG AAAACTGCAG AAAAGACTGG TATCTTGAAC 
    GACTTGGCGA AGGGTAAGAA AAGAATGATT TTTACAGACG ATGAATTAGC CAAAGATAAT 
    GATATTTACA TTGATGAAAA CATCATGATC AAAGATAAGG ATTCTGCCGC TGATGCGGAC 
    GATTTAGAAA GCGAGTTGAA TGCCATGTAT AGTGATTATA AAACTAGAAG GTCGGAAAGG 
    GATGCCAAGT TTAGAGCTAA GCAAGCACGT GGTGGCGATA ATGAGGAAGA ATGGACCGGT 
    TTTAATGAGG GAAGTCTAGA AAAGAAAGAA GAGGAAGGTA AAGATTATAT CGAAGACAAT 
    GACGATGAAG GTGTCGAAGG AGACTCCGAT GATGATGAAG CCATCACCAA TTTGATTAGC 
    AAATTGAAGG GACAAGAAGG TGATCACAAA TTGAGTAGTA AGGCACGTAT GATTTTCAAT 
    GATCCGATAT TTAATAACGT TGAACCTGAT TTGCCAGTAA ATACCGTCAA TGATGGCATA 
    ATGAGTTCCG AGTCTGTTGG TGATATTTCT AAATTAAATA AGAAAAGAAA ACATGAAGAA 
    ATGCACCAGA AACAAGATGA AGCAGATTCT TCGGATGAGA GTTCAAGTGA TGACTCCGAT 
    TTCGAAATTG TGGCCAACGA TAATGCATCA GAAGAATTCG ATTCTGATTA TGATTCAGAG 
    GAAGAAAAAA ATCAAACAAA GAAAGAAAAG CATTCCAGAG ACATTGATAT TGCTACTGTT 
    GAAGCCATGA CTTTGGCACA TCAGTTAGCA TTGGGTCAGA AAAACAAGCA TGATCTTGTT 
    GATGAAGGTT TCAATAGATA CACCTTCCGT GACACCGAAA ATTTGCCAGA TTGGTTTTTA 
    GAAGATGAAA AGGAACATTC AAAGATAAAT AAGCCGATTA CTAAGGAGGC AGCGATGGCA 
    ATTAAAGAAA AAATAAAGGC AATGAACGCC CGTCCTATTA AGAAGGTTGC TGAAGCTAAG 
    GCAAGAAAGA GGATGCGTGC CGTAGCTCGG TTAGAAAAGA TCAAGAAGAA GGCGGGCTTG 
    ATTAACGATG ATTCAGACAA GACAGAGAAG GACAAGGCTG AAGAAATTTC TAGATTGATG 
    CGTAAGGTTA CCAAAAAACC AAAGACCAAG CCAAAGGTTA CTTTGGTTGT TGCCTCAGGT 
    AGGAACAAAG GTTTGGCAGG TAGACCAAAG GGCGTTAAGG GTAAGTATAA GATGGTTGAT 
    GGTGTCATGA AGAATGAACA AAGAGCCCTA AGACGTATCG CAAAGAAGCA TCACAAGAAA 
    AAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


    The complete DNA sequence of yeast chromosome III.
    Nature357(6373)38-46(1992 May)
    Oliver SG,van der Aart QJ,Agostoni-Carbone ML,Aigle M,Alberghina L,Alexandraki D,Antoine G,Anwar R,Ballesta JP,Benit P


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