DNF1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	DNF1
Entrez Gene ID	856913
Full Name	aminophospholipid-translocating P4-type ATPase DNF1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179056.3	NP_011093.3	aminophospholipid-translocating P4-type ATPase DNF1

Pathways from BioSystems

Homologies

Homo sapiens (human)	ATP10A	NP_077816.1
Pan troglodytes (chimpanzee)	ATP10A	XP_510255.2
Canis lupus familiaris (dog)	ATP10A	XP_545808.4
Bos taurus (cattle)	ATP10A	XP_005221762.1
	ATP10A	XP_001108767.1
Magnaporthe oryzae (rice blast fungus)	MGG_02767	XP_003720981.1
Neurospora crassa	NCU07443	XP_964630.1
Danio rerio (zebrafish)	atp10a	XP_695556.2
Arabidopsis thaliana (thale cress)	ALA1	NP_568146.1
Schizosaccharomyces pombe (fission yeast)	SPAC24B11.12c	NP_592849.1
Mus musculus (house mouse)	Atp10a	NP_033858.2
Rattus norvegicus (Norway rat)	Atp10a	NP_001135407.1
Gallus gallus (chicken)	ATP10A	XP_416881.3
Saccharomyces cerevisiae (baker's yeast)	DNF1	NP_011093.3
Saccharomyces cerevisiae (baker's yeast)	DNF2	NP_010378.1
Kluyveromyces lactis	KLLA0C17644g	XP_452988.1
Eremothecium gossypii	AGOS_AGR120C	NP_986786.2
Oryza sativa (rice)	Os01g0277600	NP_001042736.2
Xenopus tropicalis (tropical clawed frog)	atp10a	XP_002931688.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome V.
Dietrich FS, Mulligan J, Hennessy K, Yelton MA, Allen E, Araujo R, Aviles E, Berno A, Brennan T, Carpenter J, Chen E, Cherry JM, Chung E, Duncan M, Guzman E, Hartzell G, Hunicke-Smith S, Hyman RW, Kayser A, Komp C, Lashkari D, Lew H, Lin D, Mosedale D, Davis RW
Nature387(6632 Suppl)78-81(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Dnf1 poorly recognizes diacylated phospholipid and define key substitutions enabling recognition of endogenous PS.
Title: Type IV P-type ATPases distinguish mono- versus diacyl phosphatidylserine using a cytofacial exit gate in the membrane domain.

Studie identified mutations that cause a gain of phosphatidylserine (PS) flip by type IV P-type ATPase Dnf1 or a loss of PS flip by type IV P-type ATPase Drs2.
Title: Two-gate mechanism for phospholipid selection and transport by type IV P-type ATPases.

Data show that Lem3-Dnf1/Dnf2 complex regulates the polar Cdc42 dynamics and phosphatidylserine distribution.
Title: Flippase-mediated phospholipid asymmetry promotes fast Cdc42 recycling in dynamic maintenance of cell?polarity.

aminophospholipid translocases, DNF1 and DNF2, are not required for 7-nitrobenz-2-oxa-1,3-diazol-4-yl-phosphatidylserine flip across the plasma membrane of Saccharomyces cerevisiae
Title: The putative aminophospholipid translocases, DNF1 and DNF2, are not required for 7-nitrobenz-2-oxa-1,3-diazol-4-yl-phosphatidylserine flip across the plasma membrane of Saccharomyces cerevisiae.

The ELM pathway for lyso-PtdEtn requires the action of Dnf1p and Dnf2p and their requisite beta-subunit, Lem3p, for the active uptake of lyso-PtdEtn. In addition, Ale1p, mediates the acylation of the imported lysolipid to form PtdEtn.
Title: Lysophosphatidylcholine metabolism in Saccharomyces cerevisiae: the role of P-type ATPases in transport and a broad specificity acyltransferase in acylation.

The following DNF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DNF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi05661	NM_001179056.3 Latest version!	Saccharomyces cerevisiae S288C aminophospholipid-translocating P4-type ATPase DNF1 (DNF1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DNF1 ( NM_001179056.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011093.3 Saccharomyces cerevisiae S288C aminophospholipid-translocating P4-type ATPase DNF1 (DNF1), partial mRNA.

Price & Availability↑

CloneID	OSi05661
Clone ID Related Accession (Same CDS sequence)	NM_001179056.3
Accession Version	NM_001179056.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4716bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	aminophospholipid-translocating P4-type ATPase DNF1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001137). On Jul 30, 2012 this sequence version replaced NM_001179056.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCTGGAA CTTTTCATGG CGATGGGCAT GCTCCCATGT CGCCCTTTGA AGATACATTT 
CAATTTGAAG ATAATAGCAG TAATGAAGAT ACACATATTG CACCTACCCA TTTTGATGAT 
GGTGCAACAA GCAACAAATA CAGCCGGCCA CAGGTCAGCT TCAATGATGA AACACCCAAA 
AATAAACGTG AAGATGCAGA AGAATTCACA TTTAACGATG ACACAGAATA TGACAATCAT 
TCCTTTCAGC CAACACCGAA ACTTAATAAT GGATCTGGGA CGTTTGATGA TGTGGAATTA 
GACAATGATA GTGGTGAACC GCACACTAAC TACGATGGTA TGAAAAGATT CCGGATGGGT 
ACAAAAAGGA ATAAAAAAGG AAACCCAATA ATGGGAAGAT CTAAGACCTT GAAATGGGCT 
AGGAAGAACA TTCCAAACCC ATTTGAAGAC TTTACGAAGG ATGATATTGA CCCTGGTGCC 
ATAAATCGTG CGCAAGAACT AAGGACTGTG TATTACAATA TGCCCTTACC AAAAGATATG 
ATAGATGAAG AAGGCAACCC CATTATGCAG TATCCTCGTA ATAAAATCAG AACTACCAAA 
TATACCCCGT TAACATTCTT ACCAAAAAAC ATTCTATTTC AGTTTCATAA TTTCGCCAAC 
GTTTATTTCT TAGTTTTAAT AATTTTAGGT GCATTTCAAA TTTTTGGTGT CACGAACCCT 
GGTTTAAGTG CGGTTCCGTT AGTTGTCATT GTTATAATCA CTGCGATCAA GGATGCTATT 
GAAGACTCAA GAAGAACCGT CCTAGATTTA GAGGTGAATA ATACCAAGAC ACATATTTTA 
GAAGGTGTTG AGAACGAAAA TGTCTCAACC GATAATATTT CATTGTGGAG AAGGTTTAAG 
AAAGCAAACT CTAGACTCCT TTTCAAGTTT ATACAATATT GTAAAGAACA TTTAACTGAA 
GAGGGCAAAA AGAAGAGAAT GCAGAGAAAG CGTCATGAAC TAAGAGTACA AAAAACTGTT 
GGAACGAGTG GACCCAGATC CTCTCTAGAC TCCATTGACA GTTACAGAGT ATCTGCAGAT 
TATGGCCGTC CTTCATTGGA TTATGATAAT TTGGAACAAG GGGCCGGTGA AGCCAATATA 
GTTGATAGAT CCCTACCACC CAGAACTGAT TGTAAATTTG CCAAGAATTA TTGGAAAGGT 
GTCAAGGTTG GTGATATTGT TCGTATCCAT AACAATGATG AAATCCCTGC TGACATTATC 
TTACTTTCAA CGTCGGATAC AGATGGTGCC TGCTACGTAG AAACTAAAAA TTTGGATGGT 
GAAACAAACT TAAAGGTACG CCAATCCTTG AAGTGTACCA ATACTATAAG AACGTCAAAA 
GATATTGCTA GAACAAAATT CTGGATCGAA AGTGAAGGGC CCCACTCAAA TTTGTACACT 
TATCAAGGTA ATATGAAATG GAGAAATCTT GCAGATGGCG AGATACGAAA TGAGCCAATT 
ACTATTAATA ACGTTCTGCT TCGTGGTTGC ACTCTTAGAA ATACTAAGTG GGCAATGGGT 
GTTGTAATGT TTACCGGTGG TGACACAAAA ATCATGTTAA ACTCTGGCAT TACACCCACC 
AAGAAGTCCA GGATTTCAAG AGAACTAAAC TTCTCTGTGG TGATAAATTT CGTGCTACTT 
TTCATCTTAT GTTTTGTTTC TGGTATTGCA AATGGTGTAT ATTACGACAA AAAAGGCAGA 
TCACGGTTTT CCTATGAATT TGGAACAATT GCTGGGTCAG CGGCGACAAA CGGTTTCGTC 
TCATTTTGGG TTGCTGTTAT TCTTTACCAA TCTTTAGTCC CAATCTCTTT GTACATTTCT 
GTGGAGATCA TCAAAACTGC ACAAGCGGCC TTCATTTACG GAGACGTTTT ACTCTACAAT 
GCCAAATTGG ATTATCCTTG TACACCAAAA TCCTGGAATA TTTCTGACGA TTTAGGTCAA 
GTGGAATATA TATTTTCTGA TAAAACAGGT ACATTAACTC AAAATGTAAT GGAGTTTAAA 
AAATGTACTA TCAATGGTGT TTCATATGGA AGAGCTTACA CCGAAGCGTT AGCGGGATTA 
AGAAAAAGAC AAGGTATCGA CGTCGAAACC GAAGGTAGGC GTGAGAAGGC CGAAATTGCC 
AAGGATAGGG ATACAATGAT CGATGAACTG AGAGCTTTAT CAGGAAATTC ACAATTTTAT 
CCCGAAGAAG TTACCTTTGT ATCAAAGGAG TTCGTTCGCG ATTTGAAAGG GGCTAGTGGT 
GAAGTACAAC AAAGATGCTG TGAGCACTTT ATGTTAGCAT TAGCTTTATG TCACTCCGTC 
CTTGTAGAAG CAAACCCGGA CAATCCAAAA AAACTGGATT TAAAAGCTCA ATCTCCAGAC 
GAAGCAGCGT TAGTAGCCAC AGCTAGAGAT GTAGGATTCA GTTTTGTTGG AAAAACCAAA 
AAAGGGTTGA TAATTGAAAT GCAAGGTATT CAAAAAGAAT TTGAGATTCT AAATATTTTG 
GAGTTTAATT CATCGAGAAA AAGAATGTCC TGTATTGTCA AAATTCCGGG TCTGAACCCA 
GGGGACGAGC CTAGAGCACT TTTAATTTGT AAAGGTGCAG ATTCCATTAT ATACTCAAGA 
TTAAGTCGCC AGAGTGGTAG CAATAGTGAA GCTATTTTGG AAAAAACTGC TTTGCATTTA 
GAGCAGTACG CTACTGAAGG TTTAAGAACG TTATGCATAG CTCAAAGGGA GTTAAGTTGG 
TCTGAATACG AAAAATGGAA CGAAAAGTAC GATATTGCTG CTGCATCATT AGCAAACAGA 
GAGGATGAAT TAGAAGTTGT TGCAGATTCC ATTGAACGCG AATTGATATT ATTGGGCGGT 
ACAGCTATCG AAGATCGTTT ACAAGATGGT GTTCCGGATT GTATAGAGTT GCTTGCAGAG 
GCAGGAATTA AATTATGGGT TCTTACGGGT GATAAAGTTG AAACAGCTAT AAACATTGGA 
TTTTCTTGCA ACCTTTTGAA TAATGAAATG GAATTGTTAG TTATTAAAAC AACTGGTGAT 
GACGTGAAAG AATTCGGATC AGAACCAAGT GAAATTGTGG ATGCTTTACT CTCCAAATAC 
TTGAAGGAGT ATTTCAACTT AACCGGTTCA GAAGAAGAGA TTTTTGAAGC TAAAAAGGAT 
CACGAATTTC CGAAGGGTAA TTACGCCATC GTTATCGATG GTGATGCTTT GAAATTGGCG 
CTATATGGTG AAGATATAAG AAGAAAGTTT CTTTTACTCT GCAAGAATTG TAGAGCTGTC 
TTATGCTGTA GAGTATCTCC ATCTCAAAAA GCAGCCGTTG TTAAGTTAGT CAAGGATTCT 
CTAGATGTCA TGACACTAGC CATCGGCGAT GGTTCCAACG ACGTCGCCAT GATTCAATCT 
GCGGATGTTG GTATTGGTAT TGCCGGTGAA GAAGGTCGAC AAGCTGTTAT GTGCTCAGAC 
TACGCCATTG GACAATTCAG ATATTTGGCT AGATTGGTCC TCGTTCACGG TAGGTGGTCT 
TATAAGAGAC TGGCAGAAAT GATTCCTGAG TTTTTTTACA AAAACATGAT TTTTGCTTTG 
GCATTGTTCT GGTATGGTAT CTATAATGAT TTTGATGGGT CTTACCTGTA TGAATACACA 
TACATGATGT TCTATAATTT GGCATTTACA TCCCTACCAG TCATCTTCTT AGGTATTCTG 
GACCAGGACG TGAATGACAC AATATCATTA GTGGTCCCTC AGTTATACCG TGTTGGAATT 
TTAAGGAAAG AATGGAACCA AAGAAAGTTC TTATGGTATA TGTTGGACGG ATTATATCAG 
TCTATTATCT GTTTTTTTTT CCCTTACTTG GTTTATCACA AAAATATGAT AGTTACGAGC 
AATGGGTTAG GATTGGATCA TCGTTATTTT GTCGGTGTTT ATGTGACCAC TATTGCTGTA 
ATTTCGTGTA ACACATATGT TTTATTACAC CAATATAGAT GGGATTGGTT TAGTGGATTA 
TTCATTGCAC TGTCTTGCCT CGTAGTTTTT GCATGGACGG GAATATGGTC TTCTGCGATA 
GCTAGTAGAG AATTTTTCAA AGCTGCAGCG CGTATCTATG GAGCACCTTC ATTTTGGGCT 
GTTTTCTTTG TTGCCGTGCT GTTCTGCTTA CTGCCCCGTT TCACATATGA CAGTTTTCAA 
AAATTTTTCT ACCCAACTGA TGTGGAAATA GTAAGAGAGA TGTGGCAACA TGGCCATTTC 
GATCATTATC CTCCTGGTTA CGATCCAACA GATCCCAACA GACCCAAGGT CACAAAAGCT 
GGGCAACACG GAGAGAAGAT TATAGAAGGC ATAGCACTTT CCGACAACTT AGGAGGATCG 
AACTATTCAA GGGATAGTGT TGTCACAGAG GAGATTCCAA TGACTTTCAT GCACGGTGAA 
GATGGAAGTC CTAGCGGATA TCAGAAGCAA GAAACCTGGA TGACTTCACC CAAGGAAACA 
CAAGATCTTC TTCAATCGCC CCAATTTCAG CAGGCCCAAA CGTTCGGAAG AGGCCCATCA 
ACCAACGTCA GGTCATCCTT GGACCGCACA AGAGAACAGA TGATCGCCAC TAACCAACTA 
GATAACCGCT ACTCTGTGGA AAGAGCGAGA ACATCTCTAG ATCTACCAGG CGTAACTAAC 
GCAGCCTCCT TAATCGGAAC ACAACAGAAC AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011093.3
CDS	1..4716
Translation	MSGTFHGDGHAPMSPFEDTFQFEDNSSNEDTHIAPTHFDDGATSNKYSRPQVSFNDETPKNKREDAEEFTFNDDTEYDNHSFQPTPKLNNGSGTFDDVELDNDSGEPHTNYDGMKRFRMGTKRNKKGNPIMGRSKTLKWARKNIPNPFEDFTKDDIDPGAINRAQELRTVYYNMPLPKDMIDEEGNPIMQYPRNKIRTTKYTPLTFLPKNILFQFHNFANVYFLVLIILGAFQIFGVTNPGLSAVPLVVIVIITAIKDAIEDSRRTVLDLEVNNTKTHILEGVENENVSTDNISLWRRFKKANSRLLFKFIQYCKEHLTEEGKKKRMQRKRHELRVQKTVGTSGPRSSLDSIDSYRVSADYGRPSLDYDNLEQGAGEANIVDRSLPPRTDCKFAKNYWKGVKVGDIVRIHNNDEIPADIILLSTSDTDGACYVETKNLDGETNLKVRQSLKCTNTIRTSKDIARTKFWIESEGPHSNLYTYQGNMKWRNLADGEIRNEPITINNVLLRGCTLRNTKWAMGVVMFTGGDTKIMLNSGITPTKKSRISRELNFSVVINFVLLFILCFVSGIANGVYYDKKGRSRFSYEFGTIAGSAATNGFVSFWVAVILYQSLVPISLYISVEIIKTAQAAFIYGDVLLYNAKLDYPCTPKSWNISDDLGQVEYIFSDKTGTLTQNVMEFKKCTINGVSYGRAYTEALAGLRKRQGIDVETEGRREKAEIAKDRDTMIDELRALSGNSQFYPEEVTFVSKEFVRDLKGASGEVQQRCCEHFMLALALCHSVLVEANPDNPKKLDLKAQSPDEAALVATARDVGFSFVGKTKKGLIIEMQGIQKEFEILNILEFNSSRKRMSCIVKIPGLNPGDEPRALLICKGADSIIYSRLSRQSGSNSEAILEKTALHLEQYATEGLRTLCIAQRELSWSEYEKWNEKYDIAAASLANREDELEVVADSIERELILLGGTAIEDRLQDGVPDCIELLAEAGIKLWVLTGDKVETAINIGFSCNLLNNEMELLVIKTTGDDVKEFGSEPSEIVDALLSKYLKEYFNLTGSEEEIFEAKKDHEFPKGNYAIVIDGDALKLALYGEDIRRKFLLLCKNCRAVLCCRVSPSQKAAVVKLVKDSLDVMTLAIGDGSNDVAMIQSADVGIGIAGEEGRQAVMCSDYAIGQFRYLARLVLVHGRWSYKRLAEMIPEFFYKNMIFALALFWYGIYNDFDGSYLYEYTYMMFYNLAFTSLPVIFLGILDQDVNDTISLVVPQLYRVGILRKEWNQRKFLWYMLDGLYQSIICFFFPYLVYHKNMIVTSNGLGLDHRYFVGVYVTTIAVISCNTYVLLHQYRWDWFSGLFIALSCLVVFAWTGIWSSAIASREFFKAAARIYGAPSFWAVFFVAVLFCLLPRFTYDSFQKFFYPTDVEIVREMWQHGHFDHYPPGYDPTDPNRPKVTKAGQHGEKIIEGIALSDNLGGSNYSRDSVVTEEIPMTFMHGEDGSPSGYQKQETWMTSPKETQDLLQSPQFQQAQTFGRGPSTNVRSSLDRTREQMIATNQLDNRYSVERARTSLDLPGVTNAASLIGTQQNN

Target ORF information:

RefSeq Version	NM_001179056.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C aminophospholipid-translocating P4-type ATPase DNF1 (DNF1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179056.3

ORF Insert Sequence:

ATGTCTGGAA CTTTTCATGG CGATGGGCAT GCTCCCATGT CGCCCTTTGA AGATACATTT 
CAATTTGAAG ATAATAGCAG TAATGAAGAT ACACATATTG CACCTACCCA TTTTGATGAT 
GGTGCAACAA GCAACAAATA CAGCCGGCCA CAGGTCAGCT TCAATGATGA AACACCCAAA 
AATAAACGTG AAGATGCAGA AGAATTCACA TTTAACGATG ACACAGAATA TGACAATCAT 
TCCTTTCAGC CAACACCGAA ACTTAATAAT GGATCTGGGA CGTTTGATGA TGTGGAATTA 
GACAATGATA GTGGTGAACC GCACACTAAC TACGATGGTA TGAAAAGATT CCGGATGGGT 
ACAAAAAGGA ATAAAAAAGG AAACCCAATA ATGGGAAGAT CTAAGACCTT GAAATGGGCT 
AGGAAGAACA TTCCAAACCC ATTTGAAGAC TTTACGAAGG ATGATATTGA CCCTGGTGCC 
ATAAATCGTG CGCAAGAACT AAGGACTGTG TATTACAATA TGCCCTTACC AAAAGATATG 
ATAGATGAAG AAGGCAACCC CATTATGCAG TATCCTCGTA ATAAAATCAG AACTACCAAA 
TATACCCCGT TAACATTCTT ACCAAAAAAC ATTCTATTTC AGTTTCATAA TTTCGCCAAC 
GTTTATTTCT TAGTTTTAAT AATTTTAGGT GCATTTCAAA TTTTTGGTGT CACGAACCCT 
GGTTTAAGTG CGGTTCCGTT AGTTGTCATT GTTATAATCA CTGCGATCAA GGATGCTATT 
GAAGACTCAA GAAGAACCGT CCTAGATTTA GAGGTGAATA ATACCAAGAC ACATATTTTA 
GAAGGTGTTG AGAACGAAAA TGTCTCAACC GATAATATTT CATTGTGGAG AAGGTTTAAG 
AAAGCAAACT CTAGACTCCT TTTCAAGTTT ATACAATATT GTAAAGAACA TTTAACTGAA 
GAGGGCAAAA AGAAGAGAAT GCAGAGAAAG CGTCATGAAC TAAGAGTACA AAAAACTGTT 
GGAACGAGTG GACCCAGATC CTCTCTAGAC TCCATTGACA GTTACAGAGT ATCTGCAGAT 
TATGGCCGTC CTTCATTGGA TTATGATAAT TTGGAACAAG GGGCCGGTGA AGCCAATATA 
GTTGATAGAT CCCTACCACC CAGAACTGAT TGTAAATTTG CCAAGAATTA TTGGAAAGGT 
GTCAAGGTTG GTGATATTGT TCGTATCCAT AACAATGATG AAATCCCTGC TGACATTATC 
TTACTTTCAA CGTCGGATAC AGATGGTGCC TGCTACGTAG AAACTAAAAA TTTGGATGGT 
GAAACAAACT TAAAGGTACG CCAATCCTTG AAGTGTACCA ATACTATAAG AACGTCAAAA 
GATATTGCTA GAACAAAATT CTGGATCGAA AGTGAAGGGC CCCACTCAAA TTTGTACACT 
TATCAAGGTA ATATGAAATG GAGAAATCTT GCAGATGGCG AGATACGAAA TGAGCCAATT 
ACTATTAATA ACGTTCTGCT TCGTGGTTGC ACTCTTAGAA ATACTAAGTG GGCAATGGGT 
GTTGTAATGT TTACCGGTGG TGACACAAAA ATCATGTTAA ACTCTGGCAT TACACCCACC 
AAGAAGTCCA GGATTTCAAG AGAACTAAAC TTCTCTGTGG TGATAAATTT CGTGCTACTT 
TTCATCTTAT GTTTTGTTTC TGGTATTGCA AATGGTGTAT ATTACGACAA AAAAGGCAGA 
TCACGGTTTT CCTATGAATT TGGAACAATT GCTGGGTCAG CGGCGACAAA CGGTTTCGTC 
TCATTTTGGG TTGCTGTTAT TCTTTACCAA TCTTTAGTCC CAATCTCTTT GTACATTTCT 
GTGGAGATCA TCAAAACTGC ACAAGCGGCC TTCATTTACG GAGACGTTTT ACTCTACAAT 
GCCAAATTGG ATTATCCTTG TACACCAAAA TCCTGGAATA TTTCTGACGA TTTAGGTCAA 
GTGGAATATA TATTTTCTGA TAAAACAGGT ACATTAACTC AAAATGTAAT GGAGTTTAAA 
AAATGTACTA TCAATGGTGT TTCATATGGA AGAGCTTACA CCGAAGCGTT AGCGGGATTA 
AGAAAAAGAC AAGGTATCGA CGTCGAAACC GAAGGTAGGC GTGAGAAGGC CGAAATTGCC 
AAGGATAGGG ATACAATGAT CGATGAACTG AGAGCTTTAT CAGGAAATTC ACAATTTTAT 
CCCGAAGAAG TTACCTTTGT ATCAAAGGAG TTCGTTCGCG ATTTGAAAGG GGCTAGTGGT 
GAAGTACAAC AAAGATGCTG TGAGCACTTT ATGTTAGCAT TAGCTTTATG TCACTCCGTC 
CTTGTAGAAG CAAACCCGGA CAATCCAAAA AAACTGGATT TAAAAGCTCA ATCTCCAGAC 
GAAGCAGCGT TAGTAGCCAC AGCTAGAGAT GTAGGATTCA GTTTTGTTGG AAAAACCAAA 
AAAGGGTTGA TAATTGAAAT GCAAGGTATT CAAAAAGAAT TTGAGATTCT AAATATTTTG 
GAGTTTAATT CATCGAGAAA AAGAATGTCC TGTATTGTCA AAATTCCGGG TCTGAACCCA 
GGGGACGAGC CTAGAGCACT TTTAATTTGT AAAGGTGCAG ATTCCATTAT ATACTCAAGA 
TTAAGTCGCC AGAGTGGTAG CAATAGTGAA GCTATTTTGG AAAAAACTGC TTTGCATTTA 
GAGCAGTACG CTACTGAAGG TTTAAGAACG TTATGCATAG CTCAAAGGGA GTTAAGTTGG 
TCTGAATACG AAAAATGGAA CGAAAAGTAC GATATTGCTG CTGCATCATT AGCAAACAGA 
GAGGATGAAT TAGAAGTTGT TGCAGATTCC ATTGAACGCG AATTGATATT ATTGGGCGGT 
ACAGCTATCG AAGATCGTTT ACAAGATGGT GTTCCGGATT GTATAGAGTT GCTTGCAGAG 
GCAGGAATTA AATTATGGGT TCTTACGGGT GATAAAGTTG AAACAGCTAT AAACATTGGA 
TTTTCTTGCA ACCTTTTGAA TAATGAAATG GAATTGTTAG TTATTAAAAC AACTGGTGAT 
GACGTGAAAG AATTCGGATC AGAACCAAGT GAAATTGTGG ATGCTTTACT CTCCAAATAC 
TTGAAGGAGT ATTTCAACTT AACCGGTTCA GAAGAAGAGA TTTTTGAAGC TAAAAAGGAT 
CACGAATTTC CGAAGGGTAA TTACGCCATC GTTATCGATG GTGATGCTTT GAAATTGGCG 
CTATATGGTG AAGATATAAG AAGAAAGTTT CTTTTACTCT GCAAGAATTG TAGAGCTGTC 
TTATGCTGTA GAGTATCTCC ATCTCAAAAA GCAGCCGTTG TTAAGTTAGT CAAGGATTCT 
CTAGATGTCA TGACACTAGC CATCGGCGAT GGTTCCAACG ACGTCGCCAT GATTCAATCT 
GCGGATGTTG GTATTGGTAT TGCCGGTGAA GAAGGTCGAC AAGCTGTTAT GTGCTCAGAC 
TACGCCATTG GACAATTCAG ATATTTGGCT AGATTGGTCC TCGTTCACGG TAGGTGGTCT 
TATAAGAGAC TGGCAGAAAT GATTCCTGAG TTTTTTTACA AAAACATGAT TTTTGCTTTG 
GCATTGTTCT GGTATGGTAT CTATAATGAT TTTGATGGGT CTTACCTGTA TGAATACACA 
TACATGATGT TCTATAATTT GGCATTTACA TCCCTACCAG TCATCTTCTT AGGTATTCTG 
GACCAGGACG TGAATGACAC AATATCATTA GTGGTCCCTC AGTTATACCG TGTTGGAATT 
TTAAGGAAAG AATGGAACCA AAGAAAGTTC TTATGGTATA TGTTGGACGG ATTATATCAG 
TCTATTATCT GTTTTTTTTT CCCTTACTTG GTTTATCACA AAAATATGAT AGTTACGAGC 
AATGGGTTAG GATTGGATCA TCGTTATTTT GTCGGTGTTT ATGTGACCAC TATTGCTGTA 
ATTTCGTGTA ACACATATGT TTTATTACAC CAATATAGAT GGGATTGGTT TAGTGGATTA 
TTCATTGCAC TGTCTTGCCT CGTAGTTTTT GCATGGACGG GAATATGGTC TTCTGCGATA 
GCTAGTAGAG AATTTTTCAA AGCTGCAGCG CGTATCTATG GAGCACCTTC ATTTTGGGCT 
GTTTTCTTTG TTGCCGTGCT GTTCTGCTTA CTGCCCCGTT TCACATATGA CAGTTTTCAA 
AAATTTTTCT ACCCAACTGA TGTGGAAATA GTAAGAGAGA TGTGGCAACA TGGCCATTTC 
GATCATTATC CTCCTGGTTA CGATCCAACA GATCCCAACA GACCCAAGGT CACAAAAGCT 
GGGCAACACG GAGAGAAGAT TATAGAAGGC ATAGCACTTT CCGACAACTT AGGAGGATCG 
AACTATTCAA GGGATAGTGT TGTCACAGAG GAGATTCCAA TGACTTTCAT GCACGGTGAA 
GATGGAAGTC CTAGCGGATA TCAGAAGCAA GAAACCTGGA TGACTTCACC CAAGGAAACA 
CAAGATCTTC TTCAATCGCC CCAATTTCAG CAGGCCCAAA CGTTCGGAAG AGGCCCATCA 
ACCAACGTCA GGTCATCCTT GGACCGCACA AGAGAACAGA TGATCGCCAC TAACCAACTA 
GATAACCGCT ACTCTGTGGA AAGAGCGAGA ACATCTCTAG ATCTACCAGG CGTAACTAAC 
GCAGCCTCCT TAATCGGAAC ACAACAGAAC AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome V.
Nature387(6632 Suppl)78-81(1997 May)
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DNF1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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