ORF » Species Summary » Saccharomyces cerevisiae S288C » YEF1 cDNA ORF clone

YEF1 cDNA ORF clone, Saccharomyces cerevisiae S288C

  • Gene

  • Clones

  • gRNAs

The following YEF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the YEF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi05448 NM_001178856.1
Latest version!		 Saccharomyces cerevisiae S288C NADH/NAD(+) kinase (YEF1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    YEF1 ( NM_001178856.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010873.1 Saccharomyces cerevisiae S288C NADH/NAD(+) kinase (YEF1), partial mRNA.
    Price & Availability↑
    CloneID OSi05448
    Clone ID Related Accession (Same CDS sequence) NM_001178856.1
    Accession Version NM_001178856.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1488bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product NADH/NAD(+) kinase
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001137). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAAAACTG ATAGATTACT GATTAACGCT TCCCCGGAGA CATGTACCAA GGGAGATGCT 
    GAGATGGATA CTATGGATAC TATTGACAGA ATGACATCAG TTAAAGTTTT AGCGGAAGGC 
    AAGGTATTAA GCAACTTCGA AGAACCGGGC TTAATGAGGT GCGGTTATCA TGATGCAAAA 
    AACTGGGTCA GAAGATTATC GAGCGAAACA ATTGTCGGTG AGGACACGAG TAATTTATAC 
    CCATTTTATG TTGATACTGC ATACGATGTA AGGCGTTTGA GAAAGGATCT TATAAATGCT 
    AAGGTTGACT TGCAGGTTGA AAACCTGATA ATAATCTGCA ATATTAATGA TATTTCCACT 
    GTATTTCTCA TGAGAGAAGT GGTGGAATGG ATCTTACGCA ATTTCCATTC AATAACTGTA 
    TATGTACAAG ATATTTTTAA AAAGTCAACT CAGTTTGCTG TTGGTGACCT CTGCAAAGAC 
    AGCAATTGCA GTAAAAACAG AGTAAAGTAT TGGTCAAAAG AATTTGTTAA AAAACACGAT 
    TCATTCTTTG ACTTGATGAT TACACTAGGG GGTGATGGAA CTGTCCTTTT TGCTTCATCT 
    ATATTCACGA AAGATGTTCC GCCGATTGTT CCATTTGCCC TTGGATCATT AGGATTTCTA 
    ACAAATTTTG AATTTCAAAA TTTCAAAGAA ACGTTGAAAC ATATCTTAAC AGATGAGGTT 
    CGTATTAATT TACGAATGAG GTTGCAATGC AAACTCTACC GTAGAAATAA ACCAGAAATT 
    GATGCCGCAA CTGGGAGAAA AATATGTTAC ATCGATTTCA TCTCCGAACA TCACGTATTG 
    AACGAAGTAA CCATAGATAG AGGTCCAGCT CCTTGTTTAT CCCTATTAGA ACTCTATGGA 
    AACGACTCAC TAATGACTAA GGTTCAGGGA GATGGATTGA TTGTTGCCAC GCCTACGGGA 
    TCCACGGCAT ACTCATTGAG TGCAGGAGGC TCTTTAATAT CGCCAAGCGT AAATGCCATA 
    GCGGTGACGC CTATATGTCC TCATACTTTG AGCTTTAGGC CTATAATTTT ACCAGATAGC 
    ATGGAATTAA AAGTTAGAGT AGATATGAAC TCAAGAGGGA CGTCGTGGGT GAATTTTGAC 
    GGAAAAGATA GAGTTGAATT GAAACAGGGT GACTATGTTG TGATAACTGC AAGCCCCTAT 
    TCGGTACCGA CTATCGAGTC ATCTGCCAGT GAATTTTTTG AAAGTATCAG TAAAAATCTT 
    AATTGGAATG ACCGCGAAGA GCAGAAGCCA TTTGCACATA TTCTCTCGCC AAAAAATCAA 
    GAAAAATATA GATTAGACTC ATCGAAAAAT GGAAACGACA CCATAAGTAA TCCCCTCGAG 
    AGTTCATGCA TAAGCTCAGA TGCACAAGAT GAGGAGAGGA AATCCGTAAC GGAAACAGAA 
    ACAGAAATAG TTGTTGAACG GACTCGTCAG GCTCATTTTG CAATCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_010873.1
    CDS1..1488
    Translation

    Target ORF information:

    RefSeq Version NM_001178856.1
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C NADH/NAD(+) kinase (YEF1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001178856.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAAAACTG ATAGATTACT GATTAACGCT TCCCCGGAGA CATGTACCAA GGGAGATGCT 
    GAGATGGATA CTATGGATAC TATTGACAGA ATGACATCAG TTAAAGTTTT AGCGGAAGGC 
    AAGGTATTAA GCAACTTCGA AGAACCGGGC TTAATGAGGT GCGGTTATCA TGATGCAAAA 
    AACTGGGTCA GAAGATTATC GAGCGAAACA ATTGTCGGTG AGGACACGAG TAATTTATAC 
    CCATTTTATG TTGATACTGC ATACGATGTA AGGCGTTTGA GAAAGGATCT TATAAATGCT 
    AAGGTTGACT TGCAGGTTGA AAACCTGATA ATAATCTGCA ATATTAATGA TATTTCCACT 
    GTATTTCTCA TGAGAGAAGT GGTGGAATGG ATCTTACGCA ATTTCCATTC AATAACTGTA 
    TATGTACAAG ATATTTTTAA AAAGTCAACT CAGTTTGCTG TTGGTGACCT CTGCAAAGAC 
    AGCAATTGCA GTAAAAACAG AGTAAAGTAT TGGTCAAAAG AATTTGTTAA AAAACACGAT 
    TCATTCTTTG ACTTGATGAT TACACTAGGG GGTGATGGAA CTGTCCTTTT TGCTTCATCT 
    ATATTCACGA AAGATGTTCC GCCGATTGTT CCATTTGCCC TTGGATCATT AGGATTTCTA 
    ACAAATTTTG AATTTCAAAA TTTCAAAGAA ACGTTGAAAC ATATCTTAAC AGATGAGGTT 
    CGTATTAATT TACGAATGAG GTTGCAATGC AAACTCTACC GTAGAAATAA ACCAGAAATT 
    GATGCCGCAA CTGGGAGAAA AATATGTTAC ATCGATTTCA TCTCCGAACA TCACGTATTG 
    AACGAAGTAA CCATAGATAG AGGTCCAGCT CCTTGTTTAT CCCTATTAGA ACTCTATGGA 
    AACGACTCAC TAATGACTAA GGTTCAGGGA GATGGATTGA TTGTTGCCAC GCCTACGGGA 
    TCCACGGCAT ACTCATTGAG TGCAGGAGGC TCTTTAATAT CGCCAAGCGT AAATGCCATA 
    GCGGTGACGC CTATATGTCC TCATACTTTG AGCTTTAGGC CTATAATTTT ACCAGATAGC 
    ATGGAATTAA AAGTTAGAGT AGATATGAAC TCAAGAGGGA CGTCGTGGGT GAATTTTGAC 
    GGAAAAGATA GAGTTGAATT GAAACAGGGT GACTATGTTG TGATAACTGC AAGCCCCTAT 
    TCGGTACCGA CTATCGAGTC ATCTGCCAGT GAATTTTTTG AAAGTATCAG TAAAAATCTT 
    AATTGGAATG ACCGCGAAGA GCAGAAGCCA TTTGCACATA TTCTCTCGCC AAAAAATCAA 
    GAAAAATATA GATTAGACTC ATCGAAAAAT GGAAACGACA CCATAAGTAA TCCCCTCGAG 
    AGTTCATGCA TAAGCTCAGA TGCACAAGAT GAGGAGAGGA AATCCGTAAC GGAAACAGAA 
    ACAGAAATAG TTGTTGAACG GACTCGTCAG GCTCATTTTG CAATCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The nucleotide sequence of Saccharomyces cerevisiae chromosome V.
    Nature387(6632 Suppl)78-81(1997 May)
    Dietrich FS,Mulligan J,Hennessy K,Yelton MA,Allen E,Araujo R,Aviles E,Berno A,Brennan T,Carpenter J,Chen E,Cherry JM,Chung E,Duncan M,Guzman E,Hartzell G,Hunicke-Smith S,Hyman RW,Kayser A,Komp C,Lashkari D,Lew H,Lin D,Mosedale D,Davis RW


    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


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