POL5 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	POL5
Entrez Gene ID	856655
Full Name	DNA-directed DNA polymerase
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178870.1	NP_010859.1	DNA-directed DNA polymerase

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0D00792g	XP_453107.1
Saccharomyces cerevisiae (baker's yeast)	POL5	NP_010859.1
Schizosaccharomyces pombe (fission yeast)	pol5	NP_595917.1
Magnaporthe oryzae (rice blast fungus)	MGG_07728	XP_003712934.1
Neurospora crassa	NCU02497	XP_965091.1
Eremothecium gossypii	AGOS_ACR020C	NP_983423.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome V.
Dietrich FS, Mulligan J, Hennessy K, Yelton MA, Allen E, Araujo R, Aviles E, Berno A, Brennan T, Carpenter J, Chen E, Cherry JM, Chung E, Duncan M, Guzman E, Hartzell G, Hunicke-Smith S, Hyman RW, Kayser A, Komp C, Lashkari D, Lew H, Lin D, Mosedale D, Davis RW
Nature387(6632 Suppl)78-81(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following POL5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POL5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi05435	NM_001178870.1 Latest version!	Saccharomyces cerevisiae S288C DNA-directed DNA polymerase (POL5), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POL5 ( NM_001178870.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010859.1 Saccharomyces cerevisiae S288C DNA-directed DNA polymerase (POL5), partial mRNA.

Price & Availability↑

CloneID	OSi05435
Clone ID Related Accession (Same CDS sequence)	NM_001178870.1
Accession Version	NM_001178870.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3069bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	DNA-directed DNA polymerase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001137). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGACAGGGA AAGTCAACAG AGACCTTTTT TTTAAACTAG CTTCTGATCT TCGAGAAGAG 
CGTCTACATG CAGCTGTCGC ATTAATAAAG GACTTATCCG CATTAGATTT GCCCGACGAT 
GCAGAAGAAT GGTCTTATGT ATTGAATCGT TTAATCAAGG GTTTGTCGTC TGACAGAAAT 
AGCGCGAGGT TAGGATTTTC TTTATGTTTG ACGGAAGTAA TCAATTTAGC TGTTAATATG 
CCACCAGGTC AACGACCAAA AGGTTTAGAA TCAACGAACG AGTTCTTGAG CACCTTATCA 
ACCATTTTGA ACGTTAATGT CAATGAGGGA ACAAAAAAAT CCATGAAGGG CAAAGATGAG 
CGTGGAATTT TATTTGGGAA GTTATTTGGC TTGAAATCTC TACTAAATGA ACCTTTATTC 
TCAGAAATAT TTGTTAAAGA TTTGGAAAAA GGAAACACCG AGTTTTTCAT TCGATTCACG 
GAACAGCTAA TTGATCTGGC CCTGAAGAAA AACTGGATCA AAGAACCATG CTTTTTTACC 
CTTTTCCAGA CTATGAAAAT GCTGCTACCT TTTATGGATG AAAGTTCCGC TGAAAAAATT 
CTTCTGATAT ATGATAAATA TGACCTGACT CTGACAAATG AGGGACTGTC AACTTATTTG 
TTGCTGAAAT ATGAAGGTGA TGAAAGTCTA ATTCCCTCGG TGTTGGATTT GAAAAATCCA 
GGTTGGAAGG ATAACGACCC TCTGGCAAGA GGAAATCTGC CCCTTTTAAC AAAAGTATTG 
AGAAACTCTT CTGTAATTCC GGATGCCAAT GGAGGTTTAA AAGAGACGAA AAAACAAAAA 
AACACGAATT GGAACCCTAG GCTGCATTTT GTTTGGAGTG TTCTGCTACC GTTGTTTGGT 
AATGGAAAAT TAGAGAACAC TAGTCATATT TCGAAGAAAA GGAAGAAAAC CAACAATAAA 
AAGGTTCAGA ATTCAATTCA ATTTCCAGAG TTTTGGAAAA TGGCCGTGGA CGAATCATTT 
TTCAATGAGA AAGCTTCTAG TGAAAGAAAA TATTTAGGCT TTCTAATAAT CGACGCGGCT 
TTCAAGGCAG TTCCTGGCTC GTACATCGGG TTTTGCTTCA GCCAAAACGT TATGAGAACA 
TTGATCAACC AATCAATAGA TTCACAAAGA GTTTTAAATA AGATTTCCCA GTTAACTTTA 
GACTCCATAG TGAAGGCTTG TGAAGAAGAT TCTGCAAACA GACTAGTACC TTGTTTAAAT 
GCAATGTTAT TTGGTCCTCA CGGTTCCATA AACTTTGATA AGTTAACAAA ATCTGGCACC 
GTTTCCAAAC TTATTGCAAT TAAAGAATTA CCTTCTACTG TTCTTGCCCA ATTACTCGAT 
GTGTTTTTTC TACAGTTACA AGATAAAAAG GGAGTTTTAT CTCATACCCT TTTTGCTCTT 
GATTCCATCC TTCATATAGT TCGTGCACAC AAAGTCGAAA TCAATGACAT GGACATAATG 
AAGCCCGTGT TGAGACCCAT CGTATATATG GCTTTCTTCA AACATACATC AGATGATCTG 
AAGTTAGAAC AGCTACACGA ATTAGCCAAG GAAAGACTTT ATTCCATCCT GGGAGAGTTG 
ACAATAAACA AAGAAATACG TTGCAAAGAT CCTGAAATAA ACTCATGGCA ATATCTGACA 
TTAAAGTTAA TACTGGACAT TGAAAATTCT CATGTAGGAG ATTTAATCAA TCCACTGGAC 
GAAAATTTAG AAAATATAAA AAACGAAGCT ATATCTTGCT TGAGTAAAGT TTGCAGATCA 
AGAACTGCTC AATCTTGGGG TCTAAGCACG TTGTTATCTA TGTGCTTAGT ACAATTATAT 
GCAGGTGACA CCGATTCTAT TTCTGTGATC GAAGAACTTT GCGAATTTTC CAAACACGAG 
AATAACTCAA TGGTAGGCAT CACTGAAATC TTACTATCAC TTTTAGCCCA AAAGAAGGCC 
TTACTCAGGA AATTGAGTTT AATAATCTGG CAGCAGTTTA TTGAAGAAGT TGGTTTGGAA 
GAATTGCAAA TTTTATTAGA TATTTTAAAA GCGAGAGAAA ACAAGCAAGG TTTTGCCCAG 
CTATTCGAAG GAGAAGAAGA ATTTGAAGAA ATTAAGGAAG AAAATGATGC CAGTGAAGAC 
GAATCGAAAA CTGGTAGTGA AAGTGAAAGT GAAAGTGAGA GTGATAGCGA TGATGCTGAT 
GAAAAAGATG AAGAAGACGA GGCAAATGAA GATATTTTGA ATATTGATAA AGAGGCCACT 
AGCGCCTTAG TGAAGGCGTT AAATTTACCC GATAACATAG TTAATGATAA AGGGGAAGTT 
GACTTAGACC AACTCGAAGG GCTTTCTGAC GACGGTGGAG ACGATGAAGA TGAGGAGTCT 
ATGGATGATG AAAAAATGAT GGAATTAGAT GATCAGTTAT CCGAAATTTT CAAACGTCGT 
AAGGAAGCAT TATCAAGCAT ATCTACTGGA AACCAGAGGA AATTTGAGGT AAAACAATCT 
AGGGAAAACG TTATCTCGTT CAAGCATAGA GTTGTCGACA TGTTAGCCGT TTACGTCAAA 
TATTGTGAAA AATTGACTCT TGCTAATAAA AGTGAACATT CCAACAATTT AGGAGGCTCA 
TTAAGCAAGC TCGTTTATTT TATTATCCCA ATGCTGAAAT GTGTCAATGA AACTCTTGAT 
AGGCCACTTG CCGACAAAAT TTCTAAATTG TTGAAGGGTA AAATATTCAA GATAAAAGTA 
ACTGCATTCA AAGACATGAA CAAAGACATC GAGCTTATGG ATTTGTTGAA AAAAACACAC 
AAGCTGATGC TGACTTCTAA GCCAGGTCAG CATGCTGCAG TGTTTTATTC AATGTGCTCA 
ACCAGCTCAC TGTTTTTGAG TAAGTTATAC GTTGAAATCG GTGGTAACGA CAAGCTTGAC 
GAATTGATTG ACTTGTATAC TGCAACTACC AAAGAGTGGA TGCAAAAAGG AAAATGTGGA 
CCTAACATTT TTATTGATTT CATCAATTGG CTATCTTCAA AAAAGCAAAC TGTAATGGAT 
AAGGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_010859.1
CDS	1..3069
Translation	MTGKVNRDLFFKLASDLREERLHAAVALIKDLSALDLPDDAEEWSYVLNRLIKGLSSDRNSARLGFSLCLTEVINLAVNMPPGQRPKGLESTNEFLSTLSTILNVNVNEGTKKSMKGKDERGILFGKLFGLKSLLNEPLFSEIFVKDLEKGNTEFFIRFTEQLIDLALKKNWIKEPCFFTLFQTMKMLLPFMDESSAEKILLIYDKYDLTLTNEGLSTYLLLKYEGDESLIPSVLDLKNPGWKDNDPLARGNLPLLTKVLRNSSVIPDANGGLKETKKQKNTNWNPRLHFVWSVLLPLFGNGKLENTSHISKKRKKTNNKKVQNSIQFPEFWKMAVDESFFNEKASSERKYLGFLIIDAAFKAVPGSYIGFCFSQNVMRTLINQSIDSQRVLNKISQLTLDSIVKACEEDSANRLVPCLNAMLFGPHGSINFDKLTKSGTVSKLIAIKELPSTVLAQLLDVFFLQLQDKKGVLSHTLFALDSILHIVRAHKVEINDMDIMKPVLRPIVYMAFFKHTSDDLKLEQLHELAKERLYSILGELTINKEIRCKDPEINSWQYLTLKLILDIENSHVGDLINPLDENLENIKNEAISCLSKVCRSRTAQSWGLSTLLSMCLVQLYAGDTDSISVIEELCEFSKHENNSMVGITEILLSLLAQKKALLRKLSLIIWQQFIEEVGLEELQILLDILKARENKQGFAQLFEGEEEFEEIKEENDASEDESKTGSESESESESDSDDADEKDEEDEANEDILNIDKEATSALVKALNLPDNIVNDKGEVDLDQLEGLSDDGGDDEDEESMDDEKMMELDDQLSEIFKRRKEALSSISTGNQRKFEVKQSRENVISFKHRVVDMLAVYVKYCEKLTLANKSEHSNNLGGSLSKLVYFIIPMLKCVNETLDRPLADKISKLLKGKIFKIKVTAFKDMNKDIELMDLLKKTHKLMLTSKPGQHAAVFYSMCSTSSLFLSKLYVEIGGNDKLDELIDLYTATTKEWMQKGKCGPNIFIDFINWLSSKKQTVMDKE

Target ORF information:

RefSeq Version	NM_001178870.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C DNA-directed DNA polymerase (POL5), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178870.1

ORF Insert Sequence:

ATGACAGGGA AAGTCAACAG AGACCTTTTT TTTAAACTAG CTTCTGATCT TCGAGAAGAG 
CGTCTACATG CAGCTGTCGC ATTAATAAAG GACTTATCCG CATTAGATTT GCCCGACGAT 
GCAGAAGAAT GGTCTTATGT ATTGAATCGT TTAATCAAGG GTTTGTCGTC TGACAGAAAT 
AGCGCGAGGT TAGGATTTTC TTTATGTTTG ACGGAAGTAA TCAATTTAGC TGTTAATATG 
CCACCAGGTC AACGACCAAA AGGTTTAGAA TCAACGAACG AGTTCTTGAG CACCTTATCA 
ACCATTTTGA ACGTTAATGT CAATGAGGGA ACAAAAAAAT CCATGAAGGG CAAAGATGAG 
CGTGGAATTT TATTTGGGAA GTTATTTGGC TTGAAATCTC TACTAAATGA ACCTTTATTC 
TCAGAAATAT TTGTTAAAGA TTTGGAAAAA GGAAACACCG AGTTTTTCAT TCGATTCACG 
GAACAGCTAA TTGATCTGGC CCTGAAGAAA AACTGGATCA AAGAACCATG CTTTTTTACC 
CTTTTCCAGA CTATGAAAAT GCTGCTACCT TTTATGGATG AAAGTTCCGC TGAAAAAATT 
CTTCTGATAT ATGATAAATA TGACCTGACT CTGACAAATG AGGGACTGTC AACTTATTTG 
TTGCTGAAAT ATGAAGGTGA TGAAAGTCTA ATTCCCTCGG TGTTGGATTT GAAAAATCCA 
GGTTGGAAGG ATAACGACCC TCTGGCAAGA GGAAATCTGC CCCTTTTAAC AAAAGTATTG 
AGAAACTCTT CTGTAATTCC GGATGCCAAT GGAGGTTTAA AAGAGACGAA AAAACAAAAA 
AACACGAATT GGAACCCTAG GCTGCATTTT GTTTGGAGTG TTCTGCTACC GTTGTTTGGT 
AATGGAAAAT TAGAGAACAC TAGTCATATT TCGAAGAAAA GGAAGAAAAC CAACAATAAA 
AAGGTTCAGA ATTCAATTCA ATTTCCAGAG TTTTGGAAAA TGGCCGTGGA CGAATCATTT 
TTCAATGAGA AAGCTTCTAG TGAAAGAAAA TATTTAGGCT TTCTAATAAT CGACGCGGCT 
TTCAAGGCAG TTCCTGGCTC GTACATCGGG TTTTGCTTCA GCCAAAACGT TATGAGAACA 
TTGATCAACC AATCAATAGA TTCACAAAGA GTTTTAAATA AGATTTCCCA GTTAACTTTA 
GACTCCATAG TGAAGGCTTG TGAAGAAGAT TCTGCAAACA GACTAGTACC TTGTTTAAAT 
GCAATGTTAT TTGGTCCTCA CGGTTCCATA AACTTTGATA AGTTAACAAA ATCTGGCACC 
GTTTCCAAAC TTATTGCAAT TAAAGAATTA CCTTCTACTG TTCTTGCCCA ATTACTCGAT 
GTGTTTTTTC TACAGTTACA AGATAAAAAG GGAGTTTTAT CTCATACCCT TTTTGCTCTT 
GATTCCATCC TTCATATAGT TCGTGCACAC AAAGTCGAAA TCAATGACAT GGACATAATG 
AAGCCCGTGT TGAGACCCAT CGTATATATG GCTTTCTTCA AACATACATC AGATGATCTG 
AAGTTAGAAC AGCTACACGA ATTAGCCAAG GAAAGACTTT ATTCCATCCT GGGAGAGTTG 
ACAATAAACA AAGAAATACG TTGCAAAGAT CCTGAAATAA ACTCATGGCA ATATCTGACA 
TTAAAGTTAA TACTGGACAT TGAAAATTCT CATGTAGGAG ATTTAATCAA TCCACTGGAC 
GAAAATTTAG AAAATATAAA AAACGAAGCT ATATCTTGCT TGAGTAAAGT TTGCAGATCA 
AGAACTGCTC AATCTTGGGG TCTAAGCACG TTGTTATCTA TGTGCTTAGT ACAATTATAT 
GCAGGTGACA CCGATTCTAT TTCTGTGATC GAAGAACTTT GCGAATTTTC CAAACACGAG 
AATAACTCAA TGGTAGGCAT CACTGAAATC TTACTATCAC TTTTAGCCCA AAAGAAGGCC 
TTACTCAGGA AATTGAGTTT AATAATCTGG CAGCAGTTTA TTGAAGAAGT TGGTTTGGAA 
GAATTGCAAA TTTTATTAGA TATTTTAAAA GCGAGAGAAA ACAAGCAAGG TTTTGCCCAG 
CTATTCGAAG GAGAAGAAGA ATTTGAAGAA ATTAAGGAAG AAAATGATGC CAGTGAAGAC 
GAATCGAAAA CTGGTAGTGA AAGTGAAAGT GAAAGTGAGA GTGATAGCGA TGATGCTGAT 
GAAAAAGATG AAGAAGACGA GGCAAATGAA GATATTTTGA ATATTGATAA AGAGGCCACT 
AGCGCCTTAG TGAAGGCGTT AAATTTACCC GATAACATAG TTAATGATAA AGGGGAAGTT 
GACTTAGACC AACTCGAAGG GCTTTCTGAC GACGGTGGAG ACGATGAAGA TGAGGAGTCT 
ATGGATGATG AAAAAATGAT GGAATTAGAT GATCAGTTAT CCGAAATTTT CAAACGTCGT 
AAGGAAGCAT TATCAAGCAT ATCTACTGGA AACCAGAGGA AATTTGAGGT AAAACAATCT 
AGGGAAAACG TTATCTCGTT CAAGCATAGA GTTGTCGACA TGTTAGCCGT TTACGTCAAA 
TATTGTGAAA AATTGACTCT TGCTAATAAA AGTGAACATT CCAACAATTT AGGAGGCTCA 
TTAAGCAAGC TCGTTTATTT TATTATCCCA ATGCTGAAAT GTGTCAATGA AACTCTTGAT 
AGGCCACTTG CCGACAAAAT TTCTAAATTG TTGAAGGGTA AAATATTCAA GATAAAAGTA 
ACTGCATTCA AAGACATGAA CAAAGACATC GAGCTTATGG ATTTGTTGAA AAAAACACAC 
AAGCTGATGC TGACTTCTAA GCCAGGTCAG CATGCTGCAG TGTTTTATTC AATGTGCTCA 
ACCAGCTCAC TGTTTTTGAG TAAGTTATAC GTTGAAATCG GTGGTAACGA CAAGCTTGAC 
GAATTGATTG ACTTGTATAC TGCAACTACC AAAGAGTGGA TGCAAAAAGG AAAATGTGGA 
CCTAACATTT TTATTGATTT CATCAATTGG CTATCTTCAA AAAAGCAAAC TGTAATGGAT 
AAGGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome V.
Nature387(6632 Suppl)78-81(1997 May)
Dietrich FS,Mulligan J,Hennessy K,Yelton MA,Allen E,Araujo R,Aviles E,Berno A,Brennan T,Carpenter J,Chen E,Cherry JM,Chung E,Duncan M,Guzman E,Hartzell G,Hunicke-Smith S,Hyman RW,Kayser A,Komp C,Lashkari D,Lew H,Lin D,Mosedale D,Davis RW

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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POL5 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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