CIN8 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	CIN8
Entrez Gene ID	856648
Full Name	kinesin motor protein CIN8
Synonyms	KSL2,SDS15
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178876.1	NP_010853.2	kinesin motor protein CIN8

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_ACR010C	NP_983413.1
Saccharomyces cerevisiae (baker's yeast)	CIN8	NP_010853.2
Kluyveromyces lactis	KLLA0D01045g	XP_453117.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome V.
Dietrich FS, Mulligan J, Hennessy K, Yelton MA, Allen E, Araujo R, Aviles E, Berno A, Brennan T, Carpenter J, Chen E, Cherry JM, Chung E, Duncan M, Guzman E, Hartzell G, Hunicke-Smith S, Hyman RW, Kayser A, Komp C, Lashkari D, Lew H, Lin D, Mosedale D, Davis RW
Nature387(6632 Suppl)78-81(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Bidirectional Kinesin Cin8 Produce Additive Forces in Both Directions of Movement
Title: Ensembles of Bidirectional Kinesin Cin8 Produce Additive Forces in Both Directions of Movement.

Data suggest that approximately four Cin8 motor domains can bind each alpha/beta-tubulin dimer within microtubule lattice; Cin8 binds microtubules not only at the canonical site, but also one or more separate "non-canonical" sites.
Title: The yeast kinesin-5 Cin8 interacts with the microtubule in a noncanonical manner.

The tail domain is one of the factors that regulate Cin8 directionality.
Title: Deletion of the Tail Domain of the Kinesin-5 Cin8 Affects Its Directionality.

Cin8 mutants lacking critical CDK phosphorylation sites suppress the requirement for Cdc14 and Cdc5 in anaphase spindle elongation.
Title: FEAR-mediated activation of Cdc14 is the limiting step for spindle elongation and anaphase progression.

The deletion mutant cells exhibited reduced midzone-directed motility and efficiency to support spindle elongation, indicating the importance of directionality control for the anaphase function of Cin8.
Title: Directionality of individual kinesin-5 Cin8 motors is modulated by loop 8, ionic strength and microtubule geometry.

The following CIN8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CIN8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi05429	NM_001178876.1 Latest version!	Saccharomyces cerevisiae S288C kinesin motor protein CIN8 (CIN8), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CIN8 ( NM_001178876.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010853.2 Saccharomyces cerevisiae S288C kinesin motor protein CIN8 (CIN8), partial mRNA.

Price & Availability↑

CloneID	OSi05429
Clone ID Related Accession (Same CDS sequence)	NM_001178876.1
Accession Version	NM_001178876.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3003bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	kinesin motor protein CIN8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001137). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGCCAGCGG AAAACCAAAA TACGGGTCAA GATAGAAGCT CCAACAGCAT CAGTAAAAAT 
GGCAACTCTC AGGTTGGATG TCACACTGTT CCTAATGAGG AACTGAACAT CACTGTAGCT 
GTGCGATGCA GAGGAAGGAA TGAAAGGGAA ATTAGTATGA AAAGCTCCGT TGTGGTAAAT 
GTTCCAGATA TTACAGGTTC TAAAGAAATT TCCATTAACA CGACGGGAGA TACCGGTATA 
ACTGCTCAAA TGAATGCCAA GAGATACACA GTGGACAAAG TCTTCGGTCC CGGCGCTTCC 
CAGGATCTAA TTTTTGATGA AGTGGCGGGC CCATTATTCC AGGATTTCAT TAAAGGTTAC 
AATTGCACCG TACTGGTATA TGGTATGACG TCAACAGGTA AAACATATAC AATGACGGGC 
GACGAAAAGT TATATAATGG TGAATTGAGC GATGCAGCAG GAATTATACC GAGGGTTCTT 
TTGAAGTTGT TTGACACATT GGAACTACAA CAGAACGATT ACGTAGTAAA ATGTTCGTTC 
ATTGAACTCT ACAACGAAGA ATTGAAGGAC CTCTTGGACA GCAATAGCAA CGGCTCTAGT 
AATACTGGCT TTGACGGCCA ATTTATGAAA AAATTGAGGA TTTTTGATTC AAGCACAGCA 
AATAATACCA CTAGCAACAG TGCTAGTAGT TCCAGGAGTA ATTCTAGGAA CAGTTCTCCG 
AGGTCATTAA ATGATCTAAC ACCTAAAGCT GCTCTATTAA GAAAAAGGTT AAGGACAAAA 
TCACTGCCGA ATACCATCAA GCAACAGTAT CAACAACAAC AGGCAGTGAA TTCCAGGAAC 
AACTCTTCCT CTAACTCTGG CTCTACCACT AATAATGCTT CTAGTAACAC CAACACAAAT 
AACGGTCAAA GAAGTTCGAT GGCTCCAAAT GACCAAACTA ATGGTATATA CATCCAGAAT 
TTGCAAGAAT TTCACATAAC AAATGCTATG GAGGGGCTAA ACCTATTACA AAAAGGCTTA 
AAGCATAGGC AAGTAGCGTC CACTAAAATG AACGATTTTT CCAGTAGATC TCATACCATT 
TTTACAATCA CTTTGTATAA GAAGCATCAG GATGAACTAT TTAGAATTTC CAAAATGAAT 
CTTGTGGATT TAGCTGGTTC AGAAAACATC AACAGATCCG GAGCATTAAA TCAACGTGCC 
AAAGAAGCTG GTTCAATCAA CCAAAGTCTA TTGACGCTGG GCAGGGTCAT AAACGCACTC 
GTAGATAAAA GCGGCCATAT ACCTTTCCGT GAATCGAAAT TGACCCGCCT GCTTCAAGAT 
TCCCTGGGTG GTAATACGAA AACCGCACTA ATTGCTACTA TATCGCCTGC AAAGGTAACT 
TCTGAAGAAA CCTGCAGTAC ATTAGAGTAT GCTTCGAAGG CTAAAAACAT TAAGAACAAG 
CCGCAACTGG GTTCATTTAT AATGAAGGAT ATTTTGGTTA AAAATATAAC TATGGAATTA 
GCAAAGATTA AATCCGATTT ACTCTCTACA AAGTCCAAAG AAGGAATATA TATGAGCCAA 
GATCACTACA AAAATTTGAA CAGTGATTTA GAAAGTTATA AAAATGAAGT TCAAGAATGT 
AAAAGAGAAA TTGAAAGTTT GACATCGAAA AATGCATTGC TAGTAAAAGA TAAATTGAAG 
TCAAAAGAAA CTATTCAATC TCAAAATTGC CAAATAGAAT CATTGAAAAC TACCATAGAT 
CATTTAAGGG CACAACTAGA TAAACAGCAT AAAACTGAAA TTGAAATATC CGATTTTAAT 
AACAAACTAC AGAAGTTGAC TGAGGTAATG CAAATGGCCC TACATGATTA CAAAAAAAGA 
GAACTTGACC TTAATCAAAA GTTTGAAATG CATATTACTA AAGAAATTAA AAAATTGAAA 
TCTACACTGT TTTTACAATT AAACACTATG CAACAGGAAA GTATTCTTCA AGAGACTAAT 
ATCCAACCAA ATCTTGATAT GATCAAAAAT GAAGTACTGA CTCTTATGAG AACCATGCAA 
GAAAAAGCTG AACTAATGTA CAAAGACTGT GTGAAGAAAA TTTTAAACGA ATCTCCTAAA 
TTCTTCAATG TTGTTATTGA GAAAATCGAC ATAATAAGAG TAGATTTCCA AAAATTTTAT 
AAAAATATAG CCGAGAATCT TTCTGATATT AGCGAAGAAA ATAACAACAT GAAACAGTAC 
TTAAAAAACC ATTTTTTCAA GAATAACCAT CAAGAATTAC TGAATCGTCA TGTGGATTCT 
ACTTATGAAA ATATTGAGAA GAGAACAAAC GAGTTTGTTG AGAACTTTAA AAAGGTCCTA 
AATGACCACC TTGACGAAAA TAAAAAACTA ATAATGCAGA ATCTGACAAC TGCAACCAGC 
GCGGTTATTG ATCAAGAAAT GGATCTGTTT GAACCCAAGC GCGTTAAATG GGAAAATTCA 
TTTGATCTGA TAAATGATTG TGACTCCATG AATAACGAAT TCTATAATAG CATGGCAGCG 
ACGCTATCGC AAATCAAGAG TACTGTTGAT ACATCATCAA ATTCGATGAA TGAGTCTATT 
TCAGTCATGA AAGGACAAGT GGAAGAATCG GAGAACGCTA TATCCCTTTT GAAGAACAAT 
ACCAAATTTA ATGATCAATT TGAGCAGCTT ATTAACAAGC ATAACATGTT GAAAGATAAC 
ATTAAAAATT CGATAACATC AACACACTCT CATATAACTA ATGTGGATGA TATCTATAAT 
ACGATTGAAA ACATAATGAA AAACTATGGT AACAAGGAAA ACGCTACCAA AGACGAAATG 
ATCGAGAACA TATTGAAGGA AATACCAAAT CTAAGTAAGA AAATGCCGTT AAGGTTATCA 
AACATAAATA GCAATTCAGT GCAAAGTGTA ATATCGCCCA AAAAGCATGC AATTGAAGAT 
GAAAACAAAT CCAGTGAAAA TGTGGACAAT GAGGGCTCGA GAAAAATGTT AAAGATTGAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_010853.2
CDS	1..3003
Translation	MPAENQNTGQDRSSNSISKNGNSQVGCHTVPNEELNITVAVRCRGRNEREISMKSSVVVNVPDITGSKEISINTTGDTGITAQMNAKRYTVDKVFGPGASQDLIFDEVAGPLFQDFIKGYNCTVLVYGMTSTGKTYTMTGDEKLYNGELSDAAGIIPRVLLKLFDTLELQQNDYVVKCSFIELYNEELKDLLDSNSNGSSNTGFDGQFMKKLRIFDSSTANNTTSNSASSSRSNSRNSSPRSLNDLTPKAALLRKRLRTKSLPNTIKQQYQQQQAVNSRNNSSSNSGSTTNNASSNTNTNNGQRSSMAPNDQTNGIYIQNLQEFHITNAMEGLNLLQKGLKHRQVASTKMNDFSSRSHTIFTITLYKKHQDELFRISKMNLVDLAGSENINRSGALNQRAKEAGSINQSLLTLGRVINALVDKSGHIPFRESKLTRLLQDSLGGNTKTALIATISPAKVTSEETCSTLEYASKAKNIKNKPQLGSFIMKDILVKNITMELAKIKSDLLSTKSKEGIYMSQDHYKNLNSDLESYKNEVQECKREIESLTSKNALLVKDKLKSKETIQSQNCQIESLKTTIDHLRAQLDKQHKTEIEISDFNNKLQKLTEVMQMALHDYKKRELDLNQKFEMHITKEIKKLKSTLFLQLNTMQQESILQETNIQPNLDMIKNEVLTLMRTMQEKAELMYKDCVKKILNESPKFFNVVIEKIDIIRVDFQKFYKNIAENLSDISEENNNMKQYLKNHFFKNNHQELLNRHVDSTYENIEKRTNEFVENFKKVLNDHLDENKKLIMQNLTTATSAVIDQEMDLFEPKRVKWENSFDLINDCDSMNNEFYNSMAATLSQIKSTVDTSSNSMNESISVMKGQVEESENAISLLKNNTKFNDQFEQLINKHNMLKDNIKNSITSTHSHITNVDDIYNTIENIMKNYGNKENATKDEMIENILKEIPNLSKKMPLRLSNINSNSVQSVISPKKHAIEDENKSSENVDNEGSRKMLKIE

Target ORF information:

RefSeq Version	NM_001178876.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C kinesin motor protein CIN8 (CIN8), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178876.1

ORF Insert Sequence:

ATGCCAGCGG AAAACCAAAA TACGGGTCAA GATAGAAGCT CCAACAGCAT CAGTAAAAAT 
GGCAACTCTC AGGTTGGATG TCACACTGTT CCTAATGAGG AACTGAACAT CACTGTAGCT 
GTGCGATGCA GAGGAAGGAA TGAAAGGGAA ATTAGTATGA AAAGCTCCGT TGTGGTAAAT 
GTTCCAGATA TTACAGGTTC TAAAGAAATT TCCATTAACA CGACGGGAGA TACCGGTATA 
ACTGCTCAAA TGAATGCCAA GAGATACACA GTGGACAAAG TCTTCGGTCC CGGCGCTTCC 
CAGGATCTAA TTTTTGATGA AGTGGCGGGC CCATTATTCC AGGATTTCAT TAAAGGTTAC 
AATTGCACCG TACTGGTATA TGGTATGACG TCAACAGGTA AAACATATAC AATGACGGGC 
GACGAAAAGT TATATAATGG TGAATTGAGC GATGCAGCAG GAATTATACC GAGGGTTCTT 
TTGAAGTTGT TTGACACATT GGAACTACAA CAGAACGATT ACGTAGTAAA ATGTTCGTTC 
ATTGAACTCT ACAACGAAGA ATTGAAGGAC CTCTTGGACA GCAATAGCAA CGGCTCTAGT 
AATACTGGCT TTGACGGCCA ATTTATGAAA AAATTGAGGA TTTTTGATTC AAGCACAGCA 
AATAATACCA CTAGCAACAG TGCTAGTAGT TCCAGGAGTA ATTCTAGGAA CAGTTCTCCG 
AGGTCATTAA ATGATCTAAC ACCTAAAGCT GCTCTATTAA GAAAAAGGTT AAGGACAAAA 
TCACTGCCGA ATACCATCAA GCAACAGTAT CAACAACAAC AGGCAGTGAA TTCCAGGAAC 
AACTCTTCCT CTAACTCTGG CTCTACCACT AATAATGCTT CTAGTAACAC CAACACAAAT 
AACGGTCAAA GAAGTTCGAT GGCTCCAAAT GACCAAACTA ATGGTATATA CATCCAGAAT 
TTGCAAGAAT TTCACATAAC AAATGCTATG GAGGGGCTAA ACCTATTACA AAAAGGCTTA 
AAGCATAGGC AAGTAGCGTC CACTAAAATG AACGATTTTT CCAGTAGATC TCATACCATT 
TTTACAATCA CTTTGTATAA GAAGCATCAG GATGAACTAT TTAGAATTTC CAAAATGAAT 
CTTGTGGATT TAGCTGGTTC AGAAAACATC AACAGATCCG GAGCATTAAA TCAACGTGCC 
AAAGAAGCTG GTTCAATCAA CCAAAGTCTA TTGACGCTGG GCAGGGTCAT AAACGCACTC 
GTAGATAAAA GCGGCCATAT ACCTTTCCGT GAATCGAAAT TGACCCGCCT GCTTCAAGAT 
TCCCTGGGTG GTAATACGAA AACCGCACTA ATTGCTACTA TATCGCCTGC AAAGGTAACT 
TCTGAAGAAA CCTGCAGTAC ATTAGAGTAT GCTTCGAAGG CTAAAAACAT TAAGAACAAG 
CCGCAACTGG GTTCATTTAT AATGAAGGAT ATTTTGGTTA AAAATATAAC TATGGAATTA 
GCAAAGATTA AATCCGATTT ACTCTCTACA AAGTCCAAAG AAGGAATATA TATGAGCCAA 
GATCACTACA AAAATTTGAA CAGTGATTTA GAAAGTTATA AAAATGAAGT TCAAGAATGT 
AAAAGAGAAA TTGAAAGTTT GACATCGAAA AATGCATTGC TAGTAAAAGA TAAATTGAAG 
TCAAAAGAAA CTATTCAATC TCAAAATTGC CAAATAGAAT CATTGAAAAC TACCATAGAT 
CATTTAAGGG CACAACTAGA TAAACAGCAT AAAACTGAAA TTGAAATATC CGATTTTAAT 
AACAAACTAC AGAAGTTGAC TGAGGTAATG CAAATGGCCC TACATGATTA CAAAAAAAGA 
GAACTTGACC TTAATCAAAA GTTTGAAATG CATATTACTA AAGAAATTAA AAAATTGAAA 
TCTACACTGT TTTTACAATT AAACACTATG CAACAGGAAA GTATTCTTCA AGAGACTAAT 
ATCCAACCAA ATCTTGATAT GATCAAAAAT GAAGTACTGA CTCTTATGAG AACCATGCAA 
GAAAAAGCTG AACTAATGTA CAAAGACTGT GTGAAGAAAA TTTTAAACGA ATCTCCTAAA 
TTCTTCAATG TTGTTATTGA GAAAATCGAC ATAATAAGAG TAGATTTCCA AAAATTTTAT 
AAAAATATAG CCGAGAATCT TTCTGATATT AGCGAAGAAA ATAACAACAT GAAACAGTAC 
TTAAAAAACC ATTTTTTCAA GAATAACCAT CAAGAATTAC TGAATCGTCA TGTGGATTCT 
ACTTATGAAA ATATTGAGAA GAGAACAAAC GAGTTTGTTG AGAACTTTAA AAAGGTCCTA 
AATGACCACC TTGACGAAAA TAAAAAACTA ATAATGCAGA ATCTGACAAC TGCAACCAGC 
GCGGTTATTG ATCAAGAAAT GGATCTGTTT GAACCCAAGC GCGTTAAATG GGAAAATTCA 
TTTGATCTGA TAAATGATTG TGACTCCATG AATAACGAAT TCTATAATAG CATGGCAGCG 
ACGCTATCGC AAATCAAGAG TACTGTTGAT ACATCATCAA ATTCGATGAA TGAGTCTATT 
TCAGTCATGA AAGGACAAGT GGAAGAATCG GAGAACGCTA TATCCCTTTT GAAGAACAAT 
ACCAAATTTA ATGATCAATT TGAGCAGCTT ATTAACAAGC ATAACATGTT GAAAGATAAC 
ATTAAAAATT CGATAACATC AACACACTCT CATATAACTA ATGTGGATGA TATCTATAAT 
ACGATTGAAA ACATAATGAA AAACTATGGT AACAAGGAAA ACGCTACCAA AGACGAAATG 
ATCGAGAACA TATTGAAGGA AATACCAAAT CTAAGTAAGA AAATGCCGTT AAGGTTATCA 
AACATAAATA GCAATTCAGT GCAAAGTGTA ATATCGCCCA AAAAGCATGC AATTGAAGAT 
GAAAACAAAT CCAGTGAAAA TGTGGACAAT GAGGGCTCGA GAAAAATGTT AAAGATTGAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome V.
Nature387(6632 Suppl)78-81(1997 May)
Dietrich FS,Mulligan J,Hennessy K,Yelton MA,Allen E,Araujo R,Aviles E,Berno A,Brennan T,Carpenter J,Chen E,Cherry JM,Chung E,Duncan M,Guzman E,Hartzell G,Hunicke-Smith S,Hyman RW,Kayser A,Komp C,Lashkari D,Lew H,Lin D,Mosedale D,Davis RW

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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CIN8 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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