SET5 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	SET5
Entrez Gene ID	856614
Full Name	S-adenosylmethionine-dependent methyltransferase
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179338.1	NP_012077.1	S-adenosylmethionine-dependent methyltransferase

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_ADL387C	NP_983709.2
Kluyveromyces lactis	KLLA0A10241g	XP_451448.1
Saccharomyces cerevisiae (baker's yeast)	SET5	NP_012077.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Johnston M, Andrews S, Brinkman R, Cooper J, Ding H, Dover J, Du Z, Favello A, Fulton L, Gattung S
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Set5 functions with Set1 to promote gene repression at telomeres.
Title: The histone methyltransferases Set5 and Set1 have overlapping functions in gene silencing and telomere maintenance.

Results revealed the novel functions of SET5 and PPR1 for the improvement of yeast acetic acid tolerance, and also implicated them in oxidative stress defense and energy metabolism in Saccharomyces cerevisiae.
Title: Improved growth and ethanol fermentation of Saccharomyces cerevisiae in the presence of acetic acid by overexpression of SET5 and PPR1.

The study uncovers novel pathways in which the methyltransferase Set5 participates and, more importantly, reveals a partnership between Set1 and Set5 in transcriptional repression near repetitive DNA elements in budding yeast.
Title: Set5 and Set1 cooperate to repress gene expression at telomeres and retrotransposons.

Genetic studies revealed that Set5 functions in cellular processes that also rely on the global chromatin modifying complexes COMPASS and NuA4, which methylate H3 lysine 4 and acetylate H4 lysines 5, 8 and 12, respectively.
Title: New marks on the block: Set5 methylates H4 lysines 5, 8 and 12.

identify Set5 as the first histone H4 methyltransferase, which monomethylates the critical H4 lysine residues 5, 8 and 12 in budding yeast
Title: Methylation of H4 lysines 5, 8 and 12 by yeast Set5 calibrates chromatin stress responses.

The following SET5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SET5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi05402	NM_001179338.1 Latest version!	Saccharomyces cerevisiae S288C S-adenosylmethionine-dependent methyltransferase (SET5), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SET5 ( NM_001179338.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012077.1 Saccharomyces cerevisiae S288C S-adenosylmethionine-dependent methyltransferase (SET5), partial mRNA.

Price & Availability↑

CloneID	OSi05402
Clone ID Related Accession (Same CDS sequence)	NM_001179338.1
Accession Version	NM_001179338.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1581bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	S-adenosylmethionine-dependent methyltransferase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001140). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Veresion :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGACATTGA CTATCAAAAT AGGAACCTTA AACGATAGCG ATCAATCTGC AGTGCATAAT 
GGTACAGAAA ATGGCTCTGA TTTTCGAAAG ATAACTCCCA CAGAGGAAGA GATCTGTGAT 
GACGTAGTTT TGTTATGGAA GGAAGAGCCC GGAACTGAAG ACGCTACGAT TCAGCATCTT 
TATGACAGGA TTACGGAAAG AAATCAATCG TGGAAATTGA GTGCTTCTCG TTTTCGAAAA 
ATTCTAAATG AGCACCATCT TTATGACACA GATCTAGAGA CCGTTTCTCT ATATAAAGAT 
AAAATACATT TTCCAAAAGC CCTAGATTCT GATGCTAAGG TCGAAGTGAA ATTTATTGAT 
GACGAACATG GAAGGGGCCT TTTCGCTAAA AGAGATTTTT CCAAGGGCCA AATCATCCTG 
AAAGAAAATA AACCTATCGT ATATATTCCA CCACTGGACA AGCTTTTTTT AATATCTAAT 
GGAAAGGCAT GTGCTCGATG TGGTAAGGCG CTATATGATT TGACCCAGCA CAAAATTATG 
GTCCATTATT TGGATTGTGA AGTTTGCAAA GCTATTTGGT GCAGTGAAAA ATGTAAAAAA 
GCACATGCGT CGTTACATGA GCTTTTATAC CACTCATGGA GATCGAATAG AATAGACATT 
CTTCATGCTG GTAATTGGAA AAGATTTGTT AACTACTGTG AGAAATATTG TTTTACCGCT 
GCCTTTTCAG TTGGTCTTAT ATATGGATCC ATGCTTTTGG ATACTACAGG AGAGGTCAAA 
GAGCAATGGC AAAAATTGGC AAGCATCTCA CAAAGGGAAA GAATTAAATT AAGAGACGCC 
AGCGGTATCG GCAGTACGTT TAGTTTACTG AATGGTACAA CTGTACATAC TGAAGAGGAA 
TCTGATAATG GTACCAAAAA GGGCGTTGAG AAGAATATTG ATGACGAGAC TGTTTGGGAG 
AAGTGCTACG AATTATTTTG CGGTGCATTT CCAAAAGCTT CTGAAGAAAT AGATTTCGAG 
AAATTTTTGA CCATGATCGG GACGTTCAAT ATCAATCAAT ATAACGGCCA GGTATATCAT 
TGGATCTCCT TCATAAACCA TGATTGTGAA CCAAACGCCT ATATTGAGCA AGTAGAAGAA 
CATGAGGAAC TGAGGTTGCA TGCAAGGAAA CCAATAAAGA AGGGGGAACA AATACGTATT 
ACGTACGTCA ATCCCTTACA TGGGGTGAGG TTGAGGCGTA GGGAATTAAG AGTTAACTGG 
GGCTTCTTAT GCCAGTGTGA CCGCTGTCAA AATGAACTAT CTACATTTGA AAGAGTTCCA 
AATCTAGAGA AAAAGAATGC GGATGCTAAT TTAGGAGTAG AAAAAATAGA TTCCAATGAT 
AGTAGTGAAG ATGGCTCTAA GAAATCAACT GGTAATCGTA AATCTTCCAT GAGAGAGGCC 
CAGCCAGATT TAAAAGAAAT ACTGAAGAAT GGAAAAGAAT TTGAATTAGA CATACCAGAA 
ACTGTTGATA CACAAGGGAA TGTAAGAAAA ACCTCAGTCA GATTCGATTC AAACGTTTCG 
GTCGCAGTGG ATGAAAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012077.1
CDS	1..1581
Translation	MTLTIKIGTLNDSDQSAVHNGTENGSDFRKITPTEEEICDDVVLLWKEEPGTEDATIQHLYDRITERNQSWKLSASRFRKILNEHHLYDTDLETVSLYKDKIHFPKALDSDAKVEVKFIDDEHGRGLFAKRDFSKGQIILKENKPIVYIPPLDKLFLISNGKACARCGKALYDLTQHKIMVHYLDCEVCKAIWCSEKCKKAHASLHELLYHSWRSNRIDILHAGNWKRFVNYCEKYCFTAAFSVGLIYGSMLLDTTGEVKEQWQKLASISQRERIKLRDASGIGSTFSLLNGTTVHTEEESDNGTKKGVEKNIDDETVWEKCYELFCGAFPKASEEIDFEKFLTMIGTFNINQYNGQVYHWISFINHDCEPNAYIEQVEEHEELRLHARKPIKKGEQIRITYVNPLHGVRLRRRELRVNWGFLCQCDRCQNELSTFERVPNLEKKNADANLGVEKIDSNDSSEDGSKKSTGNRKSSMREAQPDLKEILKNGKEFELDIPETVDTQGNVRKTSVRFDSNVSVAVDER

Target ORF information:

RefSeq Version	NM_001179338.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C S-adenosylmethionine-dependent methyltransferase (SET5), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179338.1

ORF Insert Sequence:

ATGACATTGA CTATCAAAAT AGGAACCTTA AACGATAGCG ATCAATCTGC AGTGCATAAT 
GGTACAGAAA ATGGCTCTGA TTTTCGAAAG ATAACTCCCA CAGAGGAAGA GATCTGTGAT 
GACGTAGTTT TGTTATGGAA GGAAGAGCCC GGAACTGAAG ACGCTACGAT TCAGCATCTT 
TATGACAGGA TTACGGAAAG AAATCAATCG TGGAAATTGA GTGCTTCTCG TTTTCGAAAA 
ATTCTAAATG AGCACCATCT TTATGACACA GATCTAGAGA CCGTTTCTCT ATATAAAGAT 
AAAATACATT TTCCAAAAGC CCTAGATTCT GATGCTAAGG TCGAAGTGAA ATTTATTGAT 
GACGAACATG GAAGGGGCCT TTTCGCTAAA AGAGATTTTT CCAAGGGCCA AATCATCCTG 
AAAGAAAATA AACCTATCGT ATATATTCCA CCACTGGACA AGCTTTTTTT AATATCTAAT 
GGAAAGGCAT GTGCTCGATG TGGTAAGGCG CTATATGATT TGACCCAGCA CAAAATTATG 
GTCCATTATT TGGATTGTGA AGTTTGCAAA GCTATTTGGT GCAGTGAAAA ATGTAAAAAA 
GCACATGCGT CGTTACATGA GCTTTTATAC CACTCATGGA GATCGAATAG AATAGACATT 
CTTCATGCTG GTAATTGGAA AAGATTTGTT AACTACTGTG AGAAATATTG TTTTACCGCT 
GCCTTTTCAG TTGGTCTTAT ATATGGATCC ATGCTTTTGG ATACTACAGG AGAGGTCAAA 
GAGCAATGGC AAAAATTGGC AAGCATCTCA CAAAGGGAAA GAATTAAATT AAGAGACGCC 
AGCGGTATCG GCAGTACGTT TAGTTTACTG AATGGTACAA CTGTACATAC TGAAGAGGAA 
TCTGATAATG GTACCAAAAA GGGCGTTGAG AAGAATATTG ATGACGAGAC TGTTTGGGAG 
AAGTGCTACG AATTATTTTG CGGTGCATTT CCAAAAGCTT CTGAAGAAAT AGATTTCGAG 
AAATTTTTGA CCATGATCGG GACGTTCAAT ATCAATCAAT ATAACGGCCA GGTATATCAT 
TGGATCTCCT TCATAAACCA TGATTGTGAA CCAAACGCCT ATATTGAGCA AGTAGAAGAA 
CATGAGGAAC TGAGGTTGCA TGCAAGGAAA CCAATAAAGA AGGGGGAACA AATACGTATT 
ACGTACGTCA ATCCCTTACA TGGGGTGAGG TTGAGGCGTA GGGAATTAAG AGTTAACTGG 
GGCTTCTTAT GCCAGTGTGA CCGCTGTCAA AATGAACTAT CTACATTTGA AAGAGTTCCA 
AATCTAGAGA AAAAGAATGC GGATGCTAAT TTAGGAGTAG AAAAAATAGA TTCCAATGAT 
AGTAGTGAAG ATGGCTCTAA GAAATCAACT GGTAATCGTA AATCTTCCAT GAGAGAGGCC 
CAGCCAGATT TAAAAGAAAT ACTGAAGAAT GGAAAAGAAT TTGAATTAGA CATACCAGAA 
ACTGTTGATA CACAAGGGAA TGTAAGAAAA ACCTCAGTCA GATTCGATTC AAACGTTTCG 
GTCGCAGTGG ATGAAAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)
Johnston M,Andrews S,Brinkman R,Cooper J,Ding H,Dover J,Du Z,Favello A,Fulton L,Gattung S

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

SET5 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報