STB5 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	STB5
Entrez Gene ID	856583
Full Name	Stb5p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179309.3	NP_012048.3	Stb5p

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	STB5	NP_012048.3
Kluyveromyces lactis	KLLA0A09251g	XP_451405.1
Eremothecium gossypii	AGOS_AER291C	NP_985148.2

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Johnston M, Andrews S, Brinkman R, Cooper J, Ding H, Dover J, Du Z, Favello A, Fulton L, Gattung S
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings suggest a major role for the transcription factor Stb5p in maintaining a basal flux through the pentose phosphate pathway to meet the NADPH requirements for aerobic growth, and to provide the nucleotide precursors.
Title: The Saccharomyces cerevisiae zinc factor protein Stb5p is required as a basal regulator of the pentose phosphate pathway.

Stb5p functions as a transcriptionalr egulator controlling expression of the PPP genes and glucose-6-phosphate isomerase, which leads to acetaldehyde tolerance.
Title: Transcription factor Stb5p is essential for acetaldehyde tolerance in Saccharomyces cerevisiae.

YMR315W transcription is regulated by Stb5p in response to diamide induced NADPH depletion.
Title: The Saccharomyces cerevisiae YMR315W gene encodes an NADP(H)-specific oxidoreductase regulated by the transcription factor Stb5p in response to NADPH limitation.

These data suggest that Stb5 is a key player in the control of NADPH production for resistance to oxidative stress.
Title: Oxidative stress-activated zinc cluster protein Stb5 has dual activator/repressor functions required for pentose phosphate pathway regulation and NADPH production.

The following STB5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the STB5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi05374	NM_001179309.3 Latest version!	Saccharomyces cerevisiae S288C Stb5p (STB5), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

STB5 ( NM_001179309.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012048.3 Saccharomyces cerevisiae S288C Stb5p (STB5), partial mRNA.

Price & Availability↑

CloneID	OSi05374
Clone ID Related Accession (Same CDS sequence)	NM_001179309.3
Accession Version	NM_001179309.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Stb5p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001140). On Jul 30, 2012 this sequence version replaced NM_001179309.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Veresion :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGATGGTC CCAATTTTGC ACATCAAGGC GGGAGATCAC AACGTACTAC TGAATTGTAT 
TCGTGCGCAC GATGCAGAAA ATTAAAGAAG AAGTGTGGTA AACAAATACC GACATGTGCA 
AACTGCGATA AAAATGGGGC ACACTGTTCA TATCCAGGTA GAGCCCCAAG ACGTACCAAG 
AAGGAGTTAG CGGATGCTAT GCTACGAGGG GAATATGTTC CAGTGAAAAG GAACAAGAAG 
GTAGGAAAAA GCCCATTGAG CACTAAGAGC ATGCCAAACT CTTCTAGTCC GCTATCCGCA 
AATGGCGCTA TAACTCCCGG GTTTTCGCCT TACGAAAACG ATGATGCACA TAAGATGAAA 
CAGCTAAAAC CGTCAGATCC AATAAATCTT GTCATGGGGG CAAGTCCAAA TTCTAGCGAA 
GGTGTCTCAT CGCTAATTTC GGTGCTAACA TCGCTGAATG ATAATTCTAA TCCTTCTTCG 
CACTTATCCT CTAATGAAAA TTCCATGATT CCTTCCCGAT CATTGCCAGC TTCCGTGCAG 
CAAAGTTCGA CAACTTCATC ATTCGGAGGA TATAACACGC CTTCACCACT AATTAGCAGT 
CATGTGCCTG CGAACGCCCA AGCCGTACCG CTACAAAACA ACAATCGCAA TACTAGCAAC 
GGGGATAACG GCAGTAATGT TAACCACGAT AATAACAATG GCAGTACCAA CACACCGCAA 
TTGAGTCTTA CCCCATATGC AAACAATTCA GCCCCCAATG GGAAATTCGA TTCTGTGCCG 
GTTGATGCAT CCTCGATCGA ATTTGAAACT ATGTCCTGTT GCTTTAAAGG TGGTAGAACA 
ACATCGTGGG TCAGAGAGGA TGGCTCGTTC AAGTCAATTG ATAGATCCTT ACTGGACAGG 
TTCATTGCCG CATACTTCAA ACACAATCAC CGTCTATTTC CCATGATTGA TAAAATAGCA 
TTCCTAAATG ACGCCGCGAC AATTACTGAT TTCGAAAGGT TATATGACAA CAAAAACTAC 
CCTGACAGCT TTGTTTTCAA AGTATACATG ATCATGGCTA TTGGTTGTAC AACTTTACAG 
CGTGCTGGTA TGGTTTCTCA GGACGAAGAG TGTCTGAGTG AACATTTGGC TTTTTTGGCC 
ATGAAAAAAT TTCGTAGTGT TATAATTTTA CAAGATATCG AAACTGTACG ATGCCTATTG 
TTGTTGGGTA TTTATTCGTT TTTTGAGCCA AAGGGCTCCT CGTCATGGAC AATTAGTGGT 
ATCATCATGC GATTGACCAT AGGATTAGGT TTAAATAGAG AGTTAACTGC CAAAAAACTC 
AAGAGCATGT CTGCTTTAGA AGCAGAGGCA AGATATAGAG TGTTTTGGAG TGCTTACTGC 
TTTGAAAGGC TAGTATGCAC CTCGTTGGGC CGTATATCCG GGATCGACGA CGAAGACATC 
ACTGTGCCAC TACCGAGGGC GTTGTATGTG GATGAAAGAG ACGATTTAGA GATGACCAAG 
TTGATGATAT CATTAAGGAA AATGGGCGGT CGCATTTATA AACAAGTCCA CTCTGTAAGT 
GCAGGGCGAC AAAAGTTAAC CATCGAACAA AAGCAGGAAA TCATAAGTGG ATTACGCAAG 
GAGCTAGACG AAATTTATTC TCGAGAATCA GAAAGAAGGA AACTGAAAAA ATCTCAAATG 
GATCAGGTGG AGAGGGAGAA CAATTCTACT ACAAATGTAA TATCCTTCCA TAGTTCTGAG 
ATTTGGCTAG CAATGAGATA CTCCCAGTTG CAAATCTTAC TATACAGACC ATCTGCATTG 
ATGCCAAAAC CGCCCATTGA CTCACTATCC ACTCTAGGAG AGTTTTGCTT GCAAGCCTGG 
AAACATACTT ATACACTGTA CAAGAAGCGG TTATTACCCT TGAACTGGAT AACCCTTTTC 
AGAACATTAA CCATTTGTAA CACTATCTTA TACTGTCTTT GCCAGTGGAG CATCGACCTC 
ATTGAAAGTA AAATCGAAAT TCAACAGTGT GTGGAAATAC TAAGACATTT CGGTGAAAGA 
TGGATTTTTG CTATGAGATG CGCGGATGTT TTCCAAAACA TTAGCAACAC AATTCTCGAT 
ATTAGTTTAA GCCATGGTAA AGTTCCTAAT ATGGACCAAT TAACAAGAGA GTTATTTGGC 
GCCAGCGATT CATATCAAGA CATATTAGAC GAAAACAATG TTGACGTCTC TTGGGTTGAT 
AAACTTGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012048.3
CDS	1..2232
Translation	MDGPNFAHQGGRSQRTTELYSCARCRKLKKKCGKQIPTCANCDKNGAHCSYPGRAPRRTKKELADAMLRGEYVPVKRNKKVGKSPLSTKSMPNSSSPLSANGAITPGFSPYENDDAHKMKQLKPSDPINLVMGASPNSSEGVSSLISVLTSLNDNSNPSSHLSSNENSMIPSRSLPASVQQSSTTSSFGGYNTPSPLISSHVPANAQAVPLQNNNRNTSNGDNGSNVNHDNNNGSTNTPQLSLTPYANNSAPNGKFDSVPVDASSIEFETMSCCFKGGRTTSWVREDGSFKSIDRSLLDRFIAAYFKHNHRLFPMIDKIAFLNDAATITDFERLYDNKNYPDSFVFKVYMIMAIGCTTLQRAGMVSQDEECLSEHLAFLAMKKFRSVIILQDIETVRCLLLLGIYSFFEPKGSSSWTISGIIMRLTIGLGLNRELTAKKLKSMSALEAEARYRVFWSAYCFERLVCTSLGRISGIDDEDITVPLPRALYVDERDDLEMTKLMISLRKMGGRIYKQVHSVSAGRQKLTIEQKQEIISGLRKELDEIYSRESERRKLKKSQMDQVERENNSTTNVISFHSSEIWLAMRYSQLQILLYRPSALMPKPPIDSLSTLGEFCLQAWKHTYTLYKKRLLPLNWITLFRTLTICNTILYCLCQWSIDLIESKIEIQQCVEILRHFGERWIFAMRCADVFQNISNTILDISLSHGKVPNMDQLTRELFGASDSYQDILDENNVDVSWVDKLV

Target ORF information:

RefSeq Version	NM_001179309.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Stb5p (STB5), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179309.3

ORF Insert Sequence:

ATGGATGGTC CCAATTTTGC ACATCAAGGC GGGAGATCAC AACGTACTAC TGAATTGTAT 
TCGTGCGCAC GATGCAGAAA ATTAAAGAAG AAGTGTGGTA AACAAATACC GACATGTGCA 
AACTGCGATA AAAATGGGGC ACACTGTTCA TATCCAGGTA GAGCCCCAAG ACGTACCAAG 
AAGGAGTTAG CGGATGCTAT GCTACGAGGG GAATATGTTC CAGTGAAAAG GAACAAGAAG 
GTAGGAAAAA GCCCATTGAG CACTAAGAGC ATGCCAAACT CTTCTAGTCC GCTATCCGCA 
AATGGCGCTA TAACTCCCGG GTTTTCGCCT TACGAAAACG ATGATGCACA TAAGATGAAA 
CAGCTAAAAC CGTCAGATCC AATAAATCTT GTCATGGGGG CAAGTCCAAA TTCTAGCGAA 
GGTGTCTCAT CGCTAATTTC GGTGCTAACA TCGCTGAATG ATAATTCTAA TCCTTCTTCG 
CACTTATCCT CTAATGAAAA TTCCATGATT CCTTCCCGAT CATTGCCAGC TTCCGTGCAG 
CAAAGTTCGA CAACTTCATC ATTCGGAGGA TATAACACGC CTTCACCACT AATTAGCAGT 
CATGTGCCTG CGAACGCCCA AGCCGTACCG CTACAAAACA ACAATCGCAA TACTAGCAAC 
GGGGATAACG GCAGTAATGT TAACCACGAT AATAACAATG GCAGTACCAA CACACCGCAA 
TTGAGTCTTA CCCCATATGC AAACAATTCA GCCCCCAATG GGAAATTCGA TTCTGTGCCG 
GTTGATGCAT CCTCGATCGA ATTTGAAACT ATGTCCTGTT GCTTTAAAGG TGGTAGAACA 
ACATCGTGGG TCAGAGAGGA TGGCTCGTTC AAGTCAATTG ATAGATCCTT ACTGGACAGG 
TTCATTGCCG CATACTTCAA ACACAATCAC CGTCTATTTC CCATGATTGA TAAAATAGCA 
TTCCTAAATG ACGCCGCGAC AATTACTGAT TTCGAAAGGT TATATGACAA CAAAAACTAC 
CCTGACAGCT TTGTTTTCAA AGTATACATG ATCATGGCTA TTGGTTGTAC AACTTTACAG 
CGTGCTGGTA TGGTTTCTCA GGACGAAGAG TGTCTGAGTG AACATTTGGC TTTTTTGGCC 
ATGAAAAAAT TTCGTAGTGT TATAATTTTA CAAGATATCG AAACTGTACG ATGCCTATTG 
TTGTTGGGTA TTTATTCGTT TTTTGAGCCA AAGGGCTCCT CGTCATGGAC AATTAGTGGT 
ATCATCATGC GATTGACCAT AGGATTAGGT TTAAATAGAG AGTTAACTGC CAAAAAACTC 
AAGAGCATGT CTGCTTTAGA AGCAGAGGCA AGATATAGAG TGTTTTGGAG TGCTTACTGC 
TTTGAAAGGC TAGTATGCAC CTCGTTGGGC CGTATATCCG GGATCGACGA CGAAGACATC 
ACTGTGCCAC TACCGAGGGC GTTGTATGTG GATGAAAGAG ACGATTTAGA GATGACCAAG 
TTGATGATAT CATTAAGGAA AATGGGCGGT CGCATTTATA AACAAGTCCA CTCTGTAAGT 
GCAGGGCGAC AAAAGTTAAC CATCGAACAA AAGCAGGAAA TCATAAGTGG ATTACGCAAG 
GAGCTAGACG AAATTTATTC TCGAGAATCA GAAAGAAGGA AACTGAAAAA ATCTCAAATG 
GATCAGGTGG AGAGGGAGAA CAATTCTACT ACAAATGTAA TATCCTTCCA TAGTTCTGAG 
ATTTGGCTAG CAATGAGATA CTCCCAGTTG CAAATCTTAC TATACAGACC ATCTGCATTG 
ATGCCAAAAC CGCCCATTGA CTCACTATCC ACTCTAGGAG AGTTTTGCTT GCAAGCCTGG 
AAACATACTT ATACACTGTA CAAGAAGCGG TTATTACCCT TGAACTGGAT AACCCTTTTC 
AGAACATTAA CCATTTGTAA CACTATCTTA TACTGTCTTT GCCAGTGGAG CATCGACCTC 
ATTGAAAGTA AAATCGAAAT TCAACAGTGT GTGGAAATAC TAAGACATTT CGGTGAAAGA 
TGGATTTTTG CTATGAGATG CGCGGATGTT TTCCAAAACA TTAGCAACAC AATTCTCGAT 
ATTAGTTTAA GCCATGGTAA AGTTCCTAAT ATGGACCAAT TAACAAGAGA GTTATTTGGC 
GCCAGCGATT CATATCAAGA CATATTAGAC GAAAACAATG TTGACGTCTC TTGGGTTGAT 
AAACTTGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)
Johnston M,Andrews S,Brinkman R,Cooper J,Ding H,Dover J,Du Z,Favello A,Fulton L,Gattung S

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STB5 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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