WSS1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Johnston M, Andrews S, Brinkman R, Cooper J, Ding H, Dover J, Du Z, Favello A, Fulton L, Gattung S
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)

e show that SPRTN is a DNA-dependent mammalian protease required for resolving cytotoxic DNA-protein crosslinks (DPCs)- a function that had only been attributed to the metalloprotease Wss1 in budding yeast. We provide genetic evidence that SPRTN and Wss1 function distinctly in vivo to resolve DPCs
Title: SPRTN is a mammalian DNA-binding metalloprotease that resolves DNA-protein crosslinks.

Upon DNA damage Wss1/Cdc48/Doa1 ternary complex is recruited to sumoylated targets and catalyzes SUMO chain extension through a newly recognized SUMO ligase activity.
Title: Wss1 metalloprotease partners with Cdc48/Doa1 in processing genotoxic SUMO conjugates.

Data suggest that Wss1 contributes to survival of DNA-protein crosslink (DPC)-harboring cells by acting on DPCs proteolytically. Wss1 enables replication of DPC-containing DNA and thus promotes genome integrity.
Title: A DNA-dependent protease involved in DNA-protein crosslink repair.

In dividing cells, Wss1 is exclusively present in the mother cell.
Title: The Saccharomyces cerevisiae Wss1 protein is only present in mother cells.

Involement of WSS1 in the DNA damage response.
Title: Coordinated functions of WSS1, PSY2 and TOF1 in the DNA damage response.