MSH1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	MSH1
Entrez Gene ID	856520
Full Name	mismatch repair ATPase MSH1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179250.1	NP_011988.1	mismatch repair ATPase MSH1

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0F24156g	XP_456156.1
Schizosaccharomyces pombe (fission yeast)	msh1	NP_593649.2
Eremothecium gossypii	AGOS_ABR137W	NP_983084.2
Neurospora crassa	NCU07407	XP_958182.2
Saccharomyces cerevisiae (baker's yeast)	MSH1	NP_011988.1
Magnaporthe oryzae (rice blast fungus)	MGG_04466	XP_003710995.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Johnston M, Andrews S, Brinkman R, Cooper J, Ding H, Dover J, Du Z, Favello A, Fulton L, Gattung S
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results show that the role of Msh1p in BER is unlikely to be involved in the avoidance of large-scale deletions and rearrangements.
Title: Evidence that msh1p plays multiple roles in mitochondrial base excision repair.

Msh1p-enhanced homologous recombination may play an important role in the prevention of oxidative lesion-induced rearrangements of the mitochondrial genome.
Title: Msh1p counteracts oxidative lesion-induced instability of mtDNA and stimulates mitochondrial recombination in Saccharomyces cerevisiae.

The putative recombination genes CCE1, DIN7, and MHR1 have overlapping function with the mismatch repair homolog MSH1 in point mutation avoidance and suppression of aberrant recombination events.
Title: Overlapping contributions of Msh1p and putative recombination proteins Cce1p, Din7p, and Mhr1p in large-scale recombination and genome sorting events in the mitochondrial genome of Saccharomyces cerevisiae.

MSH1-dependent repair represents a significant back-up to mtBER in the repair of oxidative damage in mtDNA
Title: Repair of oxidative damage in mitochondrial DNA of Saccharomyces cerevisiae: involvement of the MSH1-dependent pathway.

The following MSH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MSH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi05318	NM_001179250.1 Latest version!	Saccharomyces cerevisiae S288C mismatch repair ATPase MSH1 (MSH1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MSH1 ( NM_001179250.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011988.1 Saccharomyces cerevisiae S288C mismatch repair ATPase MSH1 (MSH1), partial mRNA.

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CloneID	OSi05318
Clone ID Related Accession (Same CDS sequence)	NM_001179250.1
Accession Version	NM_001179250.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2880bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	mismatch repair ATPase MSH1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001140). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Veresion :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAAGCATT TCTTTAGGCT ACCGACAGCA TTCCGGCCCA TTTCTAGGGT TTCCTTACGA 
TATTCTAGTA CTGATACCGC TCAACCAAAA ATATCAAAAC TCAAAATTAG TTTTAATAAA 
ATTTCTGAAT CAAATAGCGA AAAAAAAGAT AATTTGGGTT CAATTGACAC ACGAAATTGT 
CTTTCGACTC AGCAAGATGA CAAACTATCA AGCACTGAGC CCTCGAAGGC TTCCCTTCCA 
CCATCATTAC AATATGTTCG TGACTTGATG GATTTGTATA AGGATCATGT GGTTTTAACA 
CAAATGGGGT CATTTTATGA ACTTTACTTT GAACAAGCAA TTAGATACGC TCCAGAATTA 
AATATATCAT TGACGAATCG AGCTTATAGT CATGGCAAAG TTCCATTTGC TGGGTTTCCT 
GTACACCAGT TAAGTCGACA TTTAAAAATG CTTGTTAACA ATTGCGGATA CAGTGTAACT 
ATCGCAGAGC AATTCAAAAA AAAGGACGTG GCAGATAATG AAGCCAATAA ATTCTATAGG 
AGAGTGACTA GAATCGTTAC TCCCGGCACT TTTATTGATG AAGCATTTGA AAATTTGAGG 
GAAAATACAT ATCTCCTGAA CATCGAATTT CCTGAAAACT GTATGAGTCA AGTGGCAGAC 
ACGAGTCTAA AAGTTGGTAT ATGTTGGTGT GATGTGAGTA CTGGGGAGAT ATTTGTTCAA 
CAAGTGTATC TTAGAGATTT GGTTTCTGCA ATAACAAGAA TTCAACCTAA GGAGATTTTA 
TTAGATGAAA GATTACTTGA GTTTCATATC GAGTCAGGGA CGTGGTATCC TGAACTTGTT 
GAGCTTAAAA AATTTTTTAT AAAATATCAG AAAATGCCCA GTCAACATCG CACTATTGAA 
TCATTCTATG GGCTGTTTAA TTTGGGAGGT AAAGAAGCAA CGGAAAGGCA ATTGAAAATC 
CAATTTCAAA CTTTTACTCA GAAGGAGTTA GCTGCTTTGA GGAATACATT AATATACGTA 
AGTAATCATC TACCTGATTT CTCTATTAAT TTTCAGATTC CTCAGAGACA ATTAGCAACG 
GCGATAATGC AAATTGATTC AAGAACCAGC ACTGCACTTG AATTGCATTC TACTGTAAGA 
GACAACAATA AAAAAGGCTC TCTGTTATCA TCTATAAGAA GGACAGTTAC ACCTTCAGGA 
ACAAGACTTC TGTCTCAATG GTTGAGTGGA CCTTCCCTTG ATTTGAAAGA AATTAAAAAG 
CGTCAGAAAA TTGTAGCATT TTTCAAAGAC AACCGTGATA TCACTGAAAC ACTACGGACT 
ATGTTGAAAA AAGTAAATGA TCTATCCCGT ATACTTCAAA AGTTTAGTTT CGGAAGGGGC 
GAGGCATTAG AACTTATTCA AATGGCACGT TCACTAGAGG TTTCAAGAGA AATAAGAAAA 
TATTTACTAA ATAACACGTC GTTGATGAAA GCTACATTAA AGAGTCAAAT CACACAGCTG 
ACTGAGTCTT TAAATTTTGA AAAAAATTTG ATTGATGATA TTTTGAAGTT TTTAAATGAG 
GAAGAGCTAG CAAAGTCACA AGATGCTAAA CAGAATGCAG ATGTAACTAG AATGCTTGAC 
ATAGATGTAA AAGACAAGAA AGAAAGTAAC AAAGATGAGA TTTTTGAATT AAGAGATTTT 
ATCGTAAACC CTTCGTTCAA TACCAAACTT AGGAAATTGC ATGACACTTA TCAGGGCGTT 
TGGCAAAAAA AAACTGAGTA CAATGCTTTA TTAAAAGGTT TTTTTGTTGG CGACCTAGGT 
GCTAAGACTT TCACCTTGAA GGAAAGGCAA AACGGTGAGT ATGCCCTCCA TGTGACAGGA 
ACAGCCTCTA GTTTAAAGAA AATTGATGAG TTAATTAGTA AATCGACGGA GTACCACGGA 
AGTTGCTTCC ATATTTTGCA AAAATCAAGC CAAACACGAT GGTTGAGTCA CAAAATTTGG 
ACAGACTTGG GGCACGAGTT GGAATTATTA AATTTAAAGA TTAGGAATGA AGAGGCTAAT 
ATTATTGATC TTTTTAAAAG GAAATTTATT GATAGAAGTA ACGAGGTCAG ACAAGTTGCA 
ACTACACTGG GCTATCTTGA TACCTTATCG TCCTTTGCTG TGTTAGCTAA CGAGAGAAAT 
TTAGTCTGCC CAAAAGTGGA TGAGAGCAAT AAACTAGAAG TAGTGAATGG GAGACATCTA 
ATGGTTGAAG AGGGTCTTTC CGCGCGCTCT TTGGAGACAT TCACGGCCAA TAACTGCGAA 
TTGGCGAAGG ACAATTTATG GGTAATTACC GGACCGAATA TGGGTGGTAA ATCTACATTC 
TTAAGACAGA ATGCAATTAT AGTCATTCTG GCGCAAATTG GATGTTTTGT TCCATGCAGT 
AAGGCGCGTG TGGGTATTGT AGATAAGCTT TTTAGCCGAG TTGGTTCAGC AGATGATCTG 
TACAATGAGA TGAGTACGTT CATGGTTGAG ATGATAGAAA CATCGTTCAT CTTGCAAGGA 
GCTACGGAAC GGTCTTTAGC TATTCTAGAT GAGATTGGCC GTGGGACTAG TGGTAAAGAA 
GGCATTAGCA TCGCTTATGC AACTTTAAAG TATTTGTTAG AGAACAATCA ATGCAGAACG 
CTTTTTGCTA CACATTTTGG TCAAGAACTG AAGCAAATCA TTGATAACAA ATGTTCGAAA 
GGAATGAGCG AAAAGGTCAA GTTTTACCAA AGCGGAATCA CTGATTTAGG TGGAAACAAT 
TTTTGTTACA ACCATAAGTT GAAGCCGGGC ATCTGCACGA AATCAGATGC CATTAGAGTT 
GCGGAATTGG CCGGATTTCC AATGGAAGCG TTAAAAGAAG CCCGCGAAAT ATTGGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011988.1
CDS	1..2880
Translation	MKHFFRLPTAFRPISRVSLRYSSTDTAQPKISKLKISFNKISESNSEKKDNLGSIDTRNCLSTQQDDKLSSTEPSKASLPPSLQYVRDLMDLYKDHVVLTQMGSFYELYFEQAIRYAPELNISLTNRAYSHGKVPFAGFPVHQLSRHLKMLVNNCGYSVTIAEQFKKKDVADNEANKFYRRVTRIVTPGTFIDEAFENLRENTYLLNIEFPENCMSQVADTSLKVGICWCDVSTGEIFVQQVYLRDLVSAITRIQPKEILLDERLLEFHIESGTWYPELVELKKFFIKYQKMPSQHRTIESFYGLFNLGGKEATERQLKIQFQTFTQKELAALRNTLIYVSNHLPDFSINFQIPQRQLATAIMQIDSRTSTALELHSTVRDNNKKGSLLSSIRRTVTPSGTRLLSQWLSGPSLDLKEIKKRQKIVAFFKDNRDITETLRTMLKKVNDLSRILQKFSFGRGEALELIQMARSLEVSREIRKYLLNNTSLMKATLKSQITQLTESLNFEKNLIDDILKFLNEEELAKSQDAKQNADVTRMLDIDVKDKKESNKDEIFELRDFIVNPSFNTKLRKLHDTYQGVWQKKTEYNALLKGFFVGDLGAKTFTLKERQNGEYALHVTGTASSLKKIDELISKSTEYHGSCFHILQKSSQTRWLSHKIWTDLGHELELLNLKIRNEEANIIDLFKRKFIDRSNEVRQVATTLGYLDTLSSFAVLANERNLVCPKVDESNKLEVVNGRHLMVEEGLSARSLETFTANNCELAKDNLWVITGPNMGGKSTFLRQNAIIVILAQIGCFVPCSKARVGIVDKLFSRVGSADDLYNEMSTFMVEMIETSFILQGATERSLAILDEIGRGTSGKEGISIAYATLKYLLENNQCRTLFATHFGQELKQIIDNKCSKGMSEKVKFYQSGITDLGGNNFCYNHKLKPGICTKSDAIRVAELAGFPMEALKEAREILG

Target ORF information:

RefSeq Version	NM_001179250.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C mismatch repair ATPase MSH1 (MSH1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179250.1

ORF Insert Sequence:

ATGAAGCATT TCTTTAGGCT ACCGACAGCA TTCCGGCCCA TTTCTAGGGT TTCCTTACGA 
TATTCTAGTA CTGATACCGC TCAACCAAAA ATATCAAAAC TCAAAATTAG TTTTAATAAA 
ATTTCTGAAT CAAATAGCGA AAAAAAAGAT AATTTGGGTT CAATTGACAC ACGAAATTGT 
CTTTCGACTC AGCAAGATGA CAAACTATCA AGCACTGAGC CCTCGAAGGC TTCCCTTCCA 
CCATCATTAC AATATGTTCG TGACTTGATG GATTTGTATA AGGATCATGT GGTTTTAACA 
CAAATGGGGT CATTTTATGA ACTTTACTTT GAACAAGCAA TTAGATACGC TCCAGAATTA 
AATATATCAT TGACGAATCG AGCTTATAGT CATGGCAAAG TTCCATTTGC TGGGTTTCCT 
GTACACCAGT TAAGTCGACA TTTAAAAATG CTTGTTAACA ATTGCGGATA CAGTGTAACT 
ATCGCAGAGC AATTCAAAAA AAAGGACGTG GCAGATAATG AAGCCAATAA ATTCTATAGG 
AGAGTGACTA GAATCGTTAC TCCCGGCACT TTTATTGATG AAGCATTTGA AAATTTGAGG 
GAAAATACAT ATCTCCTGAA CATCGAATTT CCTGAAAACT GTATGAGTCA AGTGGCAGAC 
ACGAGTCTAA AAGTTGGTAT ATGTTGGTGT GATGTGAGTA CTGGGGAGAT ATTTGTTCAA 
CAAGTGTATC TTAGAGATTT GGTTTCTGCA ATAACAAGAA TTCAACCTAA GGAGATTTTA 
TTAGATGAAA GATTACTTGA GTTTCATATC GAGTCAGGGA CGTGGTATCC TGAACTTGTT 
GAGCTTAAAA AATTTTTTAT AAAATATCAG AAAATGCCCA GTCAACATCG CACTATTGAA 
TCATTCTATG GGCTGTTTAA TTTGGGAGGT AAAGAAGCAA CGGAAAGGCA ATTGAAAATC 
CAATTTCAAA CTTTTACTCA GAAGGAGTTA GCTGCTTTGA GGAATACATT AATATACGTA 
AGTAATCATC TACCTGATTT CTCTATTAAT TTTCAGATTC CTCAGAGACA ATTAGCAACG 
GCGATAATGC AAATTGATTC AAGAACCAGC ACTGCACTTG AATTGCATTC TACTGTAAGA 
GACAACAATA AAAAAGGCTC TCTGTTATCA TCTATAAGAA GGACAGTTAC ACCTTCAGGA 
ACAAGACTTC TGTCTCAATG GTTGAGTGGA CCTTCCCTTG ATTTGAAAGA AATTAAAAAG 
CGTCAGAAAA TTGTAGCATT TTTCAAAGAC AACCGTGATA TCACTGAAAC ACTACGGACT 
ATGTTGAAAA AAGTAAATGA TCTATCCCGT ATACTTCAAA AGTTTAGTTT CGGAAGGGGC 
GAGGCATTAG AACTTATTCA AATGGCACGT TCACTAGAGG TTTCAAGAGA AATAAGAAAA 
TATTTACTAA ATAACACGTC GTTGATGAAA GCTACATTAA AGAGTCAAAT CACACAGCTG 
ACTGAGTCTT TAAATTTTGA AAAAAATTTG ATTGATGATA TTTTGAAGTT TTTAAATGAG 
GAAGAGCTAG CAAAGTCACA AGATGCTAAA CAGAATGCAG ATGTAACTAG AATGCTTGAC 
ATAGATGTAA AAGACAAGAA AGAAAGTAAC AAAGATGAGA TTTTTGAATT AAGAGATTTT 
ATCGTAAACC CTTCGTTCAA TACCAAACTT AGGAAATTGC ATGACACTTA TCAGGGCGTT 
TGGCAAAAAA AAACTGAGTA CAATGCTTTA TTAAAAGGTT TTTTTGTTGG CGACCTAGGT 
GCTAAGACTT TCACCTTGAA GGAAAGGCAA AACGGTGAGT ATGCCCTCCA TGTGACAGGA 
ACAGCCTCTA GTTTAAAGAA AATTGATGAG TTAATTAGTA AATCGACGGA GTACCACGGA 
AGTTGCTTCC ATATTTTGCA AAAATCAAGC CAAACACGAT GGTTGAGTCA CAAAATTTGG 
ACAGACTTGG GGCACGAGTT GGAATTATTA AATTTAAAGA TTAGGAATGA AGAGGCTAAT 
ATTATTGATC TTTTTAAAAG GAAATTTATT GATAGAAGTA ACGAGGTCAG ACAAGTTGCA 
ACTACACTGG GCTATCTTGA TACCTTATCG TCCTTTGCTG TGTTAGCTAA CGAGAGAAAT 
TTAGTCTGCC CAAAAGTGGA TGAGAGCAAT AAACTAGAAG TAGTGAATGG GAGACATCTA 
ATGGTTGAAG AGGGTCTTTC CGCGCGCTCT TTGGAGACAT TCACGGCCAA TAACTGCGAA 
TTGGCGAAGG ACAATTTATG GGTAATTACC GGACCGAATA TGGGTGGTAA ATCTACATTC 
TTAAGACAGA ATGCAATTAT AGTCATTCTG GCGCAAATTG GATGTTTTGT TCCATGCAGT 
AAGGCGCGTG TGGGTATTGT AGATAAGCTT TTTAGCCGAG TTGGTTCAGC AGATGATCTG 
TACAATGAGA TGAGTACGTT CATGGTTGAG ATGATAGAAA CATCGTTCAT CTTGCAAGGA 
GCTACGGAAC GGTCTTTAGC TATTCTAGAT GAGATTGGCC GTGGGACTAG TGGTAAAGAA 
GGCATTAGCA TCGCTTATGC AACTTTAAAG TATTTGTTAG AGAACAATCA ATGCAGAACG 
CTTTTTGCTA CACATTTTGG TCAAGAACTG AAGCAAATCA TTGATAACAA ATGTTCGAAA 
GGAATGAGCG AAAAGGTCAA GTTTTACCAA AGCGGAATCA CTGATTTAGG TGGAAACAAT 
TTTTGTTACA ACCATAAGTT GAAGCCGGGC ATCTGCACGA AATCAGATGC CATTAGAGTT 
GCGGAATTGG CCGGATTTCC AATGGAAGCG TTAAAAGAAG CCCGCGAAAT ATTGGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)
Johnston M,Andrews S,Brinkman R,Cooper J,Ding H,Dover J,Du Z,Favello A,Fulton L,Gattung S

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MSH1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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