STE12 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	STE12
Entrez Gene ID	856484
Full Name	homeodomain family transcription factor STE12
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179214.1	NP_011952.1	homeodomain family transcription factor STE12

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0E17139g	XP_454722.1
Eremothecium gossypii	AGOS_ADR304W	NP_984400.2
Saccharomyces cerevisiae (baker's yeast)	STE12	NP_011952.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Johnston M, Andrews S, Brinkman R, Cooper J, Ding H, Dover J, Du Z, Favello A, Fulton L, Gattung S
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The Q/P-rich C-terminal region of Dhh1 was dispensable for growth at nonpermissive temperature 37 degrees C but appeared to play an important role in regulating the Ste12 protein expression and mating processes
Title: Mutational analysis of the RNA helicase Dhh1 in Ste12 expression and yeast mating.

Collectively, our results implicate Ste12 and Tec1 as general and important contributors to the Cdk8, RNAPII-CTD regulatory circuitry as it relates to the maintenance of genome integrity
Title: The RNAPII-CTD Maintains Genome Integrity through Inhibition of Retrotransposon Gene Expression and Transposition.

the transcriptional repressors Dig1 and Dig2 positively affect transcription by stabilizing Ste12
Title: Positive roles for negative regulators in the mating response of yeast.

Ste12-dependent transcription at the centromere is repressed by Dig1 protein.
Title: Endogenous transcription at the centromere facilitates centromere activity in budding yeast.

for many pheromone-responsive genes, Ste12 must activate transcription by binding to cryptic or sub-optimal sites on DNA, or may require interaction with additional uncharacterized DNA bound factors
Title: Organizational constraints on Ste12 cis-elements for a pheromone response in Saccharomyces cerevisiae.

The following STE12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the STE12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi05284	NM_001179214.1 Latest version!	Saccharomyces cerevisiae S288C homeodomain family transcription factor STE12 (STE12), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

STE12 ( NM_001179214.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011952.1 Saccharomyces cerevisiae S288C homeodomain family transcription factor STE12 (STE12), partial mRNA.

Price & Availability↑

CloneID	OSi05284
Clone ID Related Accession (Same CDS sequence)	NM_001179214.1
Accession Version	NM_001179214.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	homeodomain family transcription factor STE12
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001140). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Veresion :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAAAGTCC AAATAACCAA TAGTAGAACA GAGGAAATCT TAAAAGTTCA AGCTAATAAT 
GAAAACGATG AAGTCAGTAA AGCTACTCCG GGCGAAGTTG AAGAATCGCT AAGGTTAATC 
GGCGATCTAA AATTCTTTTT AGCCACAGCG CCGGTAAATT GGCAAGAAAA CCAAATTATA 
AGGCGATACT ATCTGAATAG TGGACAAGGC TTTGTCTCTT GTGTATTTTG GAACAATCTA 
TACTATATTA CAGGTACTGA TATTGTCAAA TGTTGTCTTT ACAGAATGCA AAAGTTTGGG 
AGAGAAGTAG TTCAAAAGAA AAAATTTGAA GAGGGTATTT TTTCAGATTT AAGAAATCTC 
AAATGTGGTA TAGATGCAAC TTTAGAACAA CCAAAGTCCG AATTTTTGTC GTTTCTATTC 
AGAAATATGT GTCTGAAAAC CCAAAAAAAG CAGAAAGTAT TTTTTTGGTT CAGTGTAGCA 
CATGATAAGT TGTTTGCGGA TGCGTTGGAA AGAGATTTAA AAAGAGAAAG TTTGAATCAG 
CCTTCAACGA CTAAGCCCGT TAATGAGCCC GCCTTATCTT TTTCATATGA TTCCTCATCT 
GATAAGCCTC TCTACGATCA GTTACTTCAA CATTTAGATT CTAGAAGACC ATCTAGTACA 
ACAAAATCAG ATAATTCGCC TCCAAAATTA GAAAGCGAGA ATTTTAAGGA TAATGAGTTG 
GTAACAGTAA CTAATCAGCC GCTTTTAGGC GTTGGCCTCA TGGATGACGA TGCGCCAGAA 
TCCCCCTCTC AAATTAATGA TTTTATTCCT CAGAAATTGA TTATAGAACC CAATACTCTC 
GAATTGAATG GTCTCACAGA AGAAACGCCT CATGACTTAC CCAAGAATAC CGCTAAGGGC 
AGAGACGAAG AAGATTTTCC TCTCGACTAT TTTCCTGTAT CTGTTGAATA CCCTACGGAG 
GAAAATGCGT TTGATCCGTT CCCTCCACAG GCTTTTACGC CAGCTGCCCC TTCCATGCCT 
ATTTCCTATG ATAACGTGAA TGAAAGGGAT TCTATGCCCG TTAATTCTCT TCTTAATAGA 
TACCCCTATC AGTTATCAGT GGCACCCACT TTCCCAGTGC CACCATCATC ATCGAGGCAA 
CATTTTATGA CAAATCGGGA TTTTTATTCA TCTAACAATA ACAAGGAAAA ATTGGTATCT 
CCTAGCGACC CTACCAGCTA CATGAAGTAT GACGAACCAG TTATGGATTT TGATGAATCT 
CGGCCAAATG AAAACTGTAC AAATGCAAAA TCTCACAACT CTGGCCAGCA AACTAAACAA 
CACCAATTAT ATTCTAACAA CTTCCAGCAA TCTTACCCAA ACGGAATGGT TCCAGGATAC 
TACCCAAAAA TGCCGTATAA TCCCATGGGG GGGGATCCTC TACTCGATCA AGCCTTTTAT 
GGCGCGGACG ATTTTTTCTT TCCACCAGAA GGATGTGATA ACAATATGCT GTATCCACAA 
ACTGCAACTT CATGGAATGT TTTGCCCCCT CAAGCTATGC AACCAGCTCC AACCTATGTT 
GGGAGACCAT ACACACCGAA TTATAGATCG ACACCAGGTT CCGCGATGTT CCCATACATG 
CAAAGTTCAA ATTCCATGCA GTGGAACACT GCTGTTTCAC CTTATAGTTC GAGAGCACCA 
TCTACAACTG CTAAAAACTA TCCTCCTAGC ACATTTTATT CTCAAAATAT AAATCAATAC 
CCACGGCGAA GAACTGTGGG AATGAAGTCT TCACAAGGAA ATGTTCCAAC AGGTAATAAA 
CAATCTGTGG GCAAGTCTGC AAAAATTTCA AAGCCTCTAC ATATTAAGAC AAGTGCTTAT 
CAGAAGCAAT ACAAAATCAA CTTGGAAACG AAAGCCAGGC CAAGTGCTGG TGACGAAGAT 
TCTGCTCATC CTGATAAGAA CAAAGAAATT TCGATGCCTA CTCCGGATTC CAATACTTTG 
GTGGTCCAGT CAGAAGAAGG TGGAGCTCAT TCACTTGAGG TAGATACCAA TCGAAGGTCC 
GATAAAAACC TTCCAGATGC AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011952.1
CDS	1..2067
Translation	MKVQITNSRTEEILKVQANNENDEVSKATPGEVEESLRLIGDLKFFLATAPVNWQENQIIRRYYLNSGQGFVSCVFWNNLYYITGTDIVKCCLYRMQKFGREVVQKKKFEEGIFSDLRNLKCGIDATLEQPKSEFLSFLFRNMCLKTQKKQKVFFWFSVAHDKLFADALERDLKRESLNQPSTTKPVNEPALSFSYDSSSDKPLYDQLLQHLDSRRPSSTTKSDNSPPKLESENFKDNELVTVTNQPLLGVGLMDDDAPESPSQINDFIPQKLIIEPNTLELNGLTEETPHDLPKNTAKGRDEEDFPLDYFPVSVEYPTEENAFDPFPPQAFTPAAPSMPISYDNVNERDSMPVNSLLNRYPYQLSVAPTFPVPPSSSRQHFMTNRDFYSSNNNKEKLVSPSDPTSYMKYDEPVMDFDESRPNENCTNAKSHNSGQQTKQHQLYSNNFQQSYPNGMVPGYYPKMPYNPMGGDPLLDQAFYGADDFFFPPEGCDNNMLYPQTATSWNVLPPQAMQPAPTYVGRPYTPNYRSTPGSAMFPYMQSSNSMQWNTAVSPYSSRAPSTTAKNYPPSTFYSQNINQYPRRRTVGMKSSQGNVPTGNKQSVGKSAKISKPLHIKTSAYQKQYKINLETKARPSAGDEDSAHPDKNKEISMPTPDSNTLVVQSEEGGAHSLEVDTNRRSDKNLPDAT

Target ORF information:

RefSeq Version	NM_001179214.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C homeodomain family transcription factor STE12 (STE12), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179214.1

ORF Insert Sequence:

ATGAAAGTCC AAATAACCAA TAGTAGAACA GAGGAAATCT TAAAAGTTCA AGCTAATAAT 
GAAAACGATG AAGTCAGTAA AGCTACTCCG GGCGAAGTTG AAGAATCGCT AAGGTTAATC 
GGCGATCTAA AATTCTTTTT AGCCACAGCG CCGGTAAATT GGCAAGAAAA CCAAATTATA 
AGGCGATACT ATCTGAATAG TGGACAAGGC TTTGTCTCTT GTGTATTTTG GAACAATCTA 
TACTATATTA CAGGTACTGA TATTGTCAAA TGTTGTCTTT ACAGAATGCA AAAGTTTGGG 
AGAGAAGTAG TTCAAAAGAA AAAATTTGAA GAGGGTATTT TTTCAGATTT AAGAAATCTC 
AAATGTGGTA TAGATGCAAC TTTAGAACAA CCAAAGTCCG AATTTTTGTC GTTTCTATTC 
AGAAATATGT GTCTGAAAAC CCAAAAAAAG CAGAAAGTAT TTTTTTGGTT CAGTGTAGCA 
CATGATAAGT TGTTTGCGGA TGCGTTGGAA AGAGATTTAA AAAGAGAAAG TTTGAATCAG 
CCTTCAACGA CTAAGCCCGT TAATGAGCCC GCCTTATCTT TTTCATATGA TTCCTCATCT 
GATAAGCCTC TCTACGATCA GTTACTTCAA CATTTAGATT CTAGAAGACC ATCTAGTACA 
ACAAAATCAG ATAATTCGCC TCCAAAATTA GAAAGCGAGA ATTTTAAGGA TAATGAGTTG 
GTAACAGTAA CTAATCAGCC GCTTTTAGGC GTTGGCCTCA TGGATGACGA TGCGCCAGAA 
TCCCCCTCTC AAATTAATGA TTTTATTCCT CAGAAATTGA TTATAGAACC CAATACTCTC 
GAATTGAATG GTCTCACAGA AGAAACGCCT CATGACTTAC CCAAGAATAC CGCTAAGGGC 
AGAGACGAAG AAGATTTTCC TCTCGACTAT TTTCCTGTAT CTGTTGAATA CCCTACGGAG 
GAAAATGCGT TTGATCCGTT CCCTCCACAG GCTTTTACGC CAGCTGCCCC TTCCATGCCT 
ATTTCCTATG ATAACGTGAA TGAAAGGGAT TCTATGCCCG TTAATTCTCT TCTTAATAGA 
TACCCCTATC AGTTATCAGT GGCACCCACT TTCCCAGTGC CACCATCATC ATCGAGGCAA 
CATTTTATGA CAAATCGGGA TTTTTATTCA TCTAACAATA ACAAGGAAAA ATTGGTATCT 
CCTAGCGACC CTACCAGCTA CATGAAGTAT GACGAACCAG TTATGGATTT TGATGAATCT 
CGGCCAAATG AAAACTGTAC AAATGCAAAA TCTCACAACT CTGGCCAGCA AACTAAACAA 
CACCAATTAT ATTCTAACAA CTTCCAGCAA TCTTACCCAA ACGGAATGGT TCCAGGATAC 
TACCCAAAAA TGCCGTATAA TCCCATGGGG GGGGATCCTC TACTCGATCA AGCCTTTTAT 
GGCGCGGACG ATTTTTTCTT TCCACCAGAA GGATGTGATA ACAATATGCT GTATCCACAA 
ACTGCAACTT CATGGAATGT TTTGCCCCCT CAAGCTATGC AACCAGCTCC AACCTATGTT 
GGGAGACCAT ACACACCGAA TTATAGATCG ACACCAGGTT CCGCGATGTT CCCATACATG 
CAAAGTTCAA ATTCCATGCA GTGGAACACT GCTGTTTCAC CTTATAGTTC GAGAGCACCA 
TCTACAACTG CTAAAAACTA TCCTCCTAGC ACATTTTATT CTCAAAATAT AAATCAATAC 
CCACGGCGAA GAACTGTGGG AATGAAGTCT TCACAAGGAA ATGTTCCAAC AGGTAATAAA 
CAATCTGTGG GCAAGTCTGC AAAAATTTCA AAGCCTCTAC ATATTAAGAC AAGTGCTTAT 
CAGAAGCAAT ACAAAATCAA CTTGGAAACG AAAGCCAGGC CAAGTGCTGG TGACGAAGAT 
TCTGCTCATC CTGATAAGAA CAAAGAAATT TCGATGCCTA CTCCGGATTC CAATACTTTG 
GTGGTCCAGT CAGAAGAAGG TGGAGCTCAT TCACTTGAGG TAGATACCAA TCGAAGGTCC 
GATAAAAACC TTCCAGATGC AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)
Johnston M,Andrews S,Brinkman R,Cooper J,Ding H,Dover J,Du Z,Favello A,Fulton L,Gattung S

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STE12 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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