KSP1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	KSP1
Entrez Gene ID	856482
Full Name	putative serine/threonine protein kinase KSP1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179212.1	NP_011950.1	putative serine/threonine protein kinase KSP1

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	KSP1	NP_011950.1
Eremothecium gossypii	AGOS_ADR300C	NP_984397.1
Kluyveromyces lactis	KLLA0E17073g	XP_454719.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Johnston M, Andrews S, Brinkman R, Cooper J, Ding H, Dover J, Du Z, Favello A, Fulton L, Gattung S
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

identifies Ksp1 as a new component that functions as part of the PKA and TORC1 signaling network to control the magnitude of autophagy.
Title: Ksp1 kinase regulates autophagy via the target of rapamycin complex 1 (TORC1) pathway.

The following KSP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KSP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi05282	NM_001179212.1 Latest version!	Saccharomyces cerevisiae S288C putative serine/threonine protein kinase KSP1 (KSP1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

KSP1 ( NM_001179212.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011950.1 Saccharomyces cerevisiae S288C putative serine/threonine protein kinase KSP1 (KSP1), partial mRNA.

Price & Availability↑

CloneID	OSi05282
Clone ID Related Accession (Same CDS sequence)	NM_001179212.1
Accession Version	NM_001179212.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	putative serine/threonine protein kinase KSP1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001140). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Veresion :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGACTTTAG ATTACGAGAT TTACAAAGAA GGAGGTATTT TGAATAATCG ATATCAAAAA 
ATAGAGGATA TCAGTGAAGG TTCATATGGT TATGTATCAT TGGCTAAAGA TGTTAGAGAG 
AAAAGATTGG TTGCAGTCAA ATACATTTTC AAATTAGAAG ATGACGGCCA GTACGATGGG 
CCTCAGGACG ACGAAAATGA TTGTGATAGT AGCGATTGTG ATGATGATGA AGACACGAAA 
GTTGATACGG ATCGCCATGA AAATGAAAAT GGTAATGCCT CCAGTAATAA CGGGTCAAGT 
AGAGAGAAGA AACACAATCT CTACAAACAC AAAAAGTCCC TAATTTCTTC GAAAGTTAAA 
TCAAGACTAT CTAATAACAT TTGTCTCGAA GCCATGTATG AAGTTGATAT TCAAACAAAA 
ATTGGTAGAC ACCAAAATAT TGCTGCTCTA TTAGATTTCT TCGACTCTTA CATCATCATG 
GAATATTGCT CTGGCGGTGA CCTATATGAG GCGATCAAAG CCGATGCTGT GCCTAAGAAA 
ACCAAATCGA TCACTCATAT TATCACACAA ATCATGGATG CAATTGAATA TGTTCATAAC 
AAGGGCATTT ATCATCGTGA TATAAAACCT GAGAACATTT TAATTTCTGG AATTGATTGG 
ACAATTAAGC TTACAGATTG GGGATTGGCT ACTACAGATA AGACGTCTAT GGATAGAAAT 
GTCGGTAGTG AAAGATATAT GTCCCCTGAA CTTTTTGATA GTAATTTGGA TATCAAGGAA 
AGAAAGGAAC CTTATGACTG TGCCAAAGTG GATTTATGGG CTATGGGAAT TGTGTTTCTT 
AATATTGTTT TCCATAAGAA TCCTTTTAGT ATTGCCAATC AATCCGACAA GTCATTCTGT 
TATTTTGCTG CTAATAGAGA AGCGTTGTTT GATGTATTTT CGACAATGGC TTATGATTTC 
TTTCAAGTAT TAAGGTATAG TTTAACCATC GATCCGGCTA ATAGAGACTT GAAAATGATG 
AGAACTGAAC TACAGAACTT ATCTGAGTAT ACATTAGACG ATGAATACTA TAATAACTTG 
GATGAAGGAT ATGAAGAAAC TATGATTGAC GGTTTGCCAC CTCAGCCTGT TCCTCCATCT 
TCAGCACCGG TATCATTACC TACTCCTATT TCATCTAGTA ACAAACAACA TATGCCTGAA 
TTTAAAAAGG ATTTCAATTT CAATAATGTG AATGAAAGGA AACGGTCAGA TGTCTCTCAA 
AATCAAAATG TTGCAAGCGG CTTCTTTAAA AAACCGTCTA CACAACAACA GAAATTTTTC 
AACCAGGGTT ATAATACAAC ACTATCTACT CATGAAAGGG CTAAATCTGC TCCTAAGTTC 
AAATTTAAGA AGCGCAATAA GTACGGAAGA ACTGATAATC AATTTTCCAA ACCAGTCAAT 
ATCGAAGATA GAAAGAAAAG TAAAATTTTG AAAAAATCAA GGAAGCCTCT TGGCATACCA 
ACGCCTAATA CACACATGAA TAATTTTTTC CACGATTACA AAGCAAGGGA TGAATTCAAC 
ACCAGAGATT TTTTCACTCC TCCAAGTGTC CAGCACAGGT ATATGGAGGG GTTTTCCAAC 
AACAACAATA AACAGTACAG ACAGAATCGT AATTACAACA ATAATAACAA CAACAGCAAT 
AACAATCATG GAAGTAATTA TAATAATTTT AACAATGGTA ATAGCTATAT AAAGGGTTGG 
AACAAAAACT TTAACAAATA CAGAAGACCA AGTTCCTCCA GTTATACCGG TAAGTCACCA 
CTTTCAAGGT ACAACATGAG CTATAATCAT AACAACAATA GTTCTATAAA TGGTTATGCA 
AGGCGTGGTT CTACGACTAC AGTTCAACAT TCACCAGGCG CTTATATTCC ACCAAATGCC 
AGGAACCATC ACGTAAGCCC AACGAATCAG TTTTTAAGAG TTCCGCAATC AACTGCTCCT 
GATATCTCTA CCGTTTTAGG TGGGAAGCCT TCATACCAGG AACACTACAC TCAGGATAGT 
ATGGATTCTG AGGGCGATCA CGATTCAGAC GACGTTTTAT TCACTCTTGA AGAAGGTGAT 
CATGATTTCG TCAATGGTAT GGACAACTTA TCTATAAATG ATCACTTACC GCATACAACG 
GTAGGTAGCC ATAATGAAGT ATTTGTTCAC GCAAGCACTA ATCACAATAA CAATGGCAAC 
AACAACCATA TCGACACCAA TAGTACCACT AATCAATATC ATAGACAATA TATTCCACCT 
CCTTTGACAA CATCATTGCA TATCAACAAT AACAATAATG AATCTAATGA ACTACCAGAT 
TTACTAAAAT CACCAGCATC ATCTGAAGCA CATTTAAATT TATCTTCTGG ACCAATTGAT 
CCAATTTTAA CAGGTAATAT TGGCAATCGA TATTCACACT CATCTGACTC TAAGGAGCTA 
GAGCAGGAGC GTAGACTCAG TATGGAACAA AAATTTAAAA ATGGGGTTTA TGTTCCACCT 
CATCATAGGA AGAGTTTCAA CTTGGGAACA CAGGTACCTC CTATGAATAT GAAGACAAGT 
AACGAGGCCA CGTTGTCAGT TTCGCATAAT TCTGTTAATT TTGGTGGATC TTACAATTCC 
AGGCGTTCCA GTGCTAATGA ATCTAACCCA CTGCATATGA ATAAAGCACT CGAGAAGTTG 
TCCTCTTCAC CAGGAGCTAA AAGCTCCTTC GTTGGCTTCC CTAAACCTTT ATTACCTCGT 
AATCATTCAA GCACTACGAT TGCGTTGCAA AATGAAGACG TATTCGCAGA CTCTAACAAT 
GACGCCATAA TTTTCGAAGA CGAAGAATAC GAAGGAGAAA GTGACAAGAT GGCACATGGC 
AAAATGGAAG GTGGTGACAA CGAATCCTCG TCAACCTCCC CGGATGAAAG GCAAATTTTC 
GGTCCTTATG AAATTTATGC TCAAACTTTC GCTGGCTCCA CTCACGATAA AAAATTAGGA 
GCTGGCAGGA AGACAAGTAT TCAGGATGAA ATGGTCGGAT CGTTAGAACA ATACAAGAAT 
AATTGGTTAA TTTTACAGCA ACAAGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011950.1
CDS	1..3090
Translation	MTLDYEIYKEGGILNNRYQKIEDISEGSYGYVSLAKDVREKRLVAVKYIFKLEDDGQYDGPQDDENDCDSSDCDDDEDTKVDTDRHENENGNASSNNGSSREKKHNLYKHKKSLISSKVKSRLSNNICLEAMYEVDIQTKIGRHQNIAALLDFFDSYIIMEYCSGGDLYEAIKADAVPKKTKSITHIITQIMDAIEYVHNKGIYHRDIKPENILISGIDWTIKLTDWGLATTDKTSMDRNVGSERYMSPELFDSNLDIKERKEPYDCAKVDLWAMGIVFLNIVFHKNPFSIANQSDKSFCYFAANREALFDVFSTMAYDFFQVLRYSLTIDPANRDLKMMRTELQNLSEYTLDDEYYNNLDEGYEETMIDGLPPQPVPPSSAPVSLPTPISSSNKQHMPEFKKDFNFNNVNERKRSDVSQNQNVASGFFKKPSTQQQKFFNQGYNTTLSTHERAKSAPKFKFKKRNKYGRTDNQFSKPVNIEDRKKSKILKKSRKPLGIPTPNTHMNNFFHDYKARDEFNTRDFFTPPSVQHRYMEGFSNNNNKQYRQNRNYNNNNNNSNNNHGSNYNNFNNGNSYIKGWNKNFNKYRRPSSSSYTGKSPLSRYNMSYNHNNNSSINGYARRGSTTTVQHSPGAYIPPNARNHHVSPTNQFLRVPQSTAPDISTVLGGKPSYQEHYTQDSMDSEGDHDSDDVLFTLEEGDHDFVNGMDNLSINDHLPHTTVGSHNEVFVHASTNHNNNGNNNHIDTNSTTNQYHRQYIPPPLTTSLHINNNNNESNELPDLLKSPASSEAHLNLSSGPIDPILTGNIGNRYSHSSDSKELEQERRLSMEQKFKNGVYVPPHHRKSFNLGTQVPPMNMKTSNEATLSVSHNSVNFGGSYNSRRSSANESNPLHMNKALEKLSSSPGAKSSFVGFPKPLLPRNHSSTTIALQNEDVFADSNNDAIIFEDEEYEGESDKMAHGKMEGGDNESSSTSPDERQIFGPYEIYAQTFAGSTHDKKLGAGRKTSIQDEMVGSLEQYKNNWLILQQQD

Target ORF information:

RefSeq Version	NM_001179212.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C putative serine/threonine protein kinase KSP1 (KSP1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179212.1

ORF Insert Sequence:

ATGACTTTAG ATTACGAGAT TTACAAAGAA GGAGGTATTT TGAATAATCG ATATCAAAAA 
ATAGAGGATA TCAGTGAAGG TTCATATGGT TATGTATCAT TGGCTAAAGA TGTTAGAGAG 
AAAAGATTGG TTGCAGTCAA ATACATTTTC AAATTAGAAG ATGACGGCCA GTACGATGGG 
CCTCAGGACG ACGAAAATGA TTGTGATAGT AGCGATTGTG ATGATGATGA AGACACGAAA 
GTTGATACGG ATCGCCATGA AAATGAAAAT GGTAATGCCT CCAGTAATAA CGGGTCAAGT 
AGAGAGAAGA AACACAATCT CTACAAACAC AAAAAGTCCC TAATTTCTTC GAAAGTTAAA 
TCAAGACTAT CTAATAACAT TTGTCTCGAA GCCATGTATG AAGTTGATAT TCAAACAAAA 
ATTGGTAGAC ACCAAAATAT TGCTGCTCTA TTAGATTTCT TCGACTCTTA CATCATCATG 
GAATATTGCT CTGGCGGTGA CCTATATGAG GCGATCAAAG CCGATGCTGT GCCTAAGAAA 
ACCAAATCGA TCACTCATAT TATCACACAA ATCATGGATG CAATTGAATA TGTTCATAAC 
AAGGGCATTT ATCATCGTGA TATAAAACCT GAGAACATTT TAATTTCTGG AATTGATTGG 
ACAATTAAGC TTACAGATTG GGGATTGGCT ACTACAGATA AGACGTCTAT GGATAGAAAT 
GTCGGTAGTG AAAGATATAT GTCCCCTGAA CTTTTTGATA GTAATTTGGA TATCAAGGAA 
AGAAAGGAAC CTTATGACTG TGCCAAAGTG GATTTATGGG CTATGGGAAT TGTGTTTCTT 
AATATTGTTT TCCATAAGAA TCCTTTTAGT ATTGCCAATC AATCCGACAA GTCATTCTGT 
TATTTTGCTG CTAATAGAGA AGCGTTGTTT GATGTATTTT CGACAATGGC TTATGATTTC 
TTTCAAGTAT TAAGGTATAG TTTAACCATC GATCCGGCTA ATAGAGACTT GAAAATGATG 
AGAACTGAAC TACAGAACTT ATCTGAGTAT ACATTAGACG ATGAATACTA TAATAACTTG 
GATGAAGGAT ATGAAGAAAC TATGATTGAC GGTTTGCCAC CTCAGCCTGT TCCTCCATCT 
TCAGCACCGG TATCATTACC TACTCCTATT TCATCTAGTA ACAAACAACA TATGCCTGAA 
TTTAAAAAGG ATTTCAATTT CAATAATGTG AATGAAAGGA AACGGTCAGA TGTCTCTCAA 
AATCAAAATG TTGCAAGCGG CTTCTTTAAA AAACCGTCTA CACAACAACA GAAATTTTTC 
AACCAGGGTT ATAATACAAC ACTATCTACT CATGAAAGGG CTAAATCTGC TCCTAAGTTC 
AAATTTAAGA AGCGCAATAA GTACGGAAGA ACTGATAATC AATTTTCCAA ACCAGTCAAT 
ATCGAAGATA GAAAGAAAAG TAAAATTTTG AAAAAATCAA GGAAGCCTCT TGGCATACCA 
ACGCCTAATA CACACATGAA TAATTTTTTC CACGATTACA AAGCAAGGGA TGAATTCAAC 
ACCAGAGATT TTTTCACTCC TCCAAGTGTC CAGCACAGGT ATATGGAGGG GTTTTCCAAC 
AACAACAATA AACAGTACAG ACAGAATCGT AATTACAACA ATAATAACAA CAACAGCAAT 
AACAATCATG GAAGTAATTA TAATAATTTT AACAATGGTA ATAGCTATAT AAAGGGTTGG 
AACAAAAACT TTAACAAATA CAGAAGACCA AGTTCCTCCA GTTATACCGG TAAGTCACCA 
CTTTCAAGGT ACAACATGAG CTATAATCAT AACAACAATA GTTCTATAAA TGGTTATGCA 
AGGCGTGGTT CTACGACTAC AGTTCAACAT TCACCAGGCG CTTATATTCC ACCAAATGCC 
AGGAACCATC ACGTAAGCCC AACGAATCAG TTTTTAAGAG TTCCGCAATC AACTGCTCCT 
GATATCTCTA CCGTTTTAGG TGGGAAGCCT TCATACCAGG AACACTACAC TCAGGATAGT 
ATGGATTCTG AGGGCGATCA CGATTCAGAC GACGTTTTAT TCACTCTTGA AGAAGGTGAT 
CATGATTTCG TCAATGGTAT GGACAACTTA TCTATAAATG ATCACTTACC GCATACAACG 
GTAGGTAGCC ATAATGAAGT ATTTGTTCAC GCAAGCACTA ATCACAATAA CAATGGCAAC 
AACAACCATA TCGACACCAA TAGTACCACT AATCAATATC ATAGACAATA TATTCCACCT 
CCTTTGACAA CATCATTGCA TATCAACAAT AACAATAATG AATCTAATGA ACTACCAGAT 
TTACTAAAAT CACCAGCATC ATCTGAAGCA CATTTAAATT TATCTTCTGG ACCAATTGAT 
CCAATTTTAA CAGGTAATAT TGGCAATCGA TATTCACACT CATCTGACTC TAAGGAGCTA 
GAGCAGGAGC GTAGACTCAG TATGGAACAA AAATTTAAAA ATGGGGTTTA TGTTCCACCT 
CATCATAGGA AGAGTTTCAA CTTGGGAACA CAGGTACCTC CTATGAATAT GAAGACAAGT 
AACGAGGCCA CGTTGTCAGT TTCGCATAAT TCTGTTAATT TTGGTGGATC TTACAATTCC 
AGGCGTTCCA GTGCTAATGA ATCTAACCCA CTGCATATGA ATAAAGCACT CGAGAAGTTG 
TCCTCTTCAC CAGGAGCTAA AAGCTCCTTC GTTGGCTTCC CTAAACCTTT ATTACCTCGT 
AATCATTCAA GCACTACGAT TGCGTTGCAA AATGAAGACG TATTCGCAGA CTCTAACAAT 
GACGCCATAA TTTTCGAAGA CGAAGAATAC GAAGGAGAAA GTGACAAGAT GGCACATGGC 
AAAATGGAAG GTGGTGACAA CGAATCCTCG TCAACCTCCC CGGATGAAAG GCAAATTTTC 
GGTCCTTATG AAATTTATGC TCAAACTTTC GCTGGCTCCA CTCACGATAA AAAATTAGGA 
GCTGGCAGGA AGACAAGTAT TCAGGATGAA ATGGTCGGAT CGTTAGAACA ATACAAGAAT 
AATTGGTTAA TTTTACAGCA ACAAGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)
Johnston M,Andrews S,Brinkman R,Cooper J,Ding H,Dover J,Du Z,Favello A,Fulton L,Gattung S

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KSP1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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