PCL5 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	PCL5
Entrez Gene ID	856468
Full Name	Pcl5p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179201.1	NP_011938.1	Pcl5p

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Johnston M, Andrews S, Brinkman R, Cooper J, Ding H, Dover J, Du Z, Favello A, Fulton L, Gattung S
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Localization of Pcl5 into the cytoplasm prevents the degradation of Gcn4.
Title: Degradation of Saccharomyces cerevisiae transcription factor Gcn4 requires a C-terminal nuclear localization signal in the cyclin Pcl5.

Data suggest that degradation of Pcl5 after self-catalyzed phosphorylation ensures that activity of the Pho85/Pcl5 complex is self-limiting in vivo.
Title: Autophosphorylation-induced degradation of the Pho85 cyclin Pcl5 is essential for response to amino acid limitation.

Dissociation of the Pho85p/Pcl5p complex is the initial step in Gcn4p stabilization.
Title: Yeast Gcn4p stabilization is initiated by the dissociation of the nuclear Pho85p/Pcl5p complex.

The following PCL5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PCL5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi05269	NM_001179201.1 Latest version!	Saccharomyces cerevisiae S288C Pcl5p (PCL5), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PCL5 ( NM_001179201.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011938.1 Saccharomyces cerevisiae S288C Pcl5p (PCL5), partial mRNA.

Price & Availability↑

CloneID	OSi05269
Clone ID Related Accession (Same CDS sequence)	NM_001179201.1
Accession Version	NM_001179201.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 690bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Pcl5p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001140). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Veresion :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGATGGAA ATCATAGGTT TACTCCAGAT TCCAAAGAAT TTAATACTGT TGTAAAAAGT 
AAAGAGTCTT CCACTGGCAG AAATCCGTAT CAGACTCCGC CCTTAGAGCA CAACGGTACT 
CATCATCAGA CAAATTACAG CAGAAAGAAA ACAAATCTAG CAATTATCAT ATCGAATTTC 
TTGTCCGAGA TCAGTCGGCC TCTTTCAAAT GGCAAAATCA ATAATTCTAC CCACAACATT 
TTAAAATTTC TAAATGAAGT GCTTAAGAGA TCGAAGTGCA GTAAAGAAAA CGCTGTGTTA 
GCCACTTTCT ACTTTCAAAA GATTCACCAA TCCAGAGGTG TCCGGGATGA GTCCTCATTG 
CCTGAATTCT CGCACTGTTC CCGCAGAATT TTTCTCTGCT GTCTTATTCT ATCGCACAAA 
TTTTTGAACG ACAACACTTA CTCGATGAAA AATTGGCAAA TTATAAGTGG GCTCCATGCA 
AAGGATCTAT CTCTAATGGA AAGATGGTGT CTGGGTAAGC TGAATTATGA GCTGGCCATT 
CCATATGACG AGTTTTTGCT ATGGGAAACG AACACTTTAA TGAAAGCGAA ATTGCGCGTC 
GGAACTCCCG CCAACGCTCC GGTGAAGAGA CCCAGAGAAT CAGACAATGA TTATGATGCA 
AATTCTTGGA AACAGATTAA ATCTTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011938.1
CDS	1..690
Translation	MDGNHRFTPDSKEFNTVVKSKESSTGRNPYQTPPLEHNGTHHQTNYSRKKTNLAIIISNFLSEISRPLSNGKINNSTHNILKFLNEVLKRSKCSKENAVLATFYFQKIHQSRGVRDESSLPEFSHCSRRIFLCCLILSHKFLNDNTYSMKNWQIISGLHAKDLSLMERWCLGKLNYELAIPYDEFLLWETNTLMKAKLRVGTPANAPVKRPRESDNDYDANSWKQIKSC

Target ORF information:

RefSeq Version	NM_001179201.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Pcl5p (PCL5), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179201.1

ORF Insert Sequence:

ATGGATGGAA ATCATAGGTT TACTCCAGAT TCCAAAGAAT TTAATACTGT TGTAAAAAGT 
AAAGAGTCTT CCACTGGCAG AAATCCGTAT CAGACTCCGC CCTTAGAGCA CAACGGTACT 
CATCATCAGA CAAATTACAG CAGAAAGAAA ACAAATCTAG CAATTATCAT ATCGAATTTC 
TTGTCCGAGA TCAGTCGGCC TCTTTCAAAT GGCAAAATCA ATAATTCTAC CCACAACATT 
TTAAAATTTC TAAATGAAGT GCTTAAGAGA TCGAAGTGCA GTAAAGAAAA CGCTGTGTTA 
GCCACTTTCT ACTTTCAAAA GATTCACCAA TCCAGAGGTG TCCGGGATGA GTCCTCATTG 
CCTGAATTCT CGCACTGTTC CCGCAGAATT TTTCTCTGCT GTCTTATTCT ATCGCACAAA 
TTTTTGAACG ACAACACTTA CTCGATGAAA AATTGGCAAA TTATAAGTGG GCTCCATGCA 
AAGGATCTAT CTCTAATGGA AAGATGGTGT CTGGGTAAGC TGAATTATGA GCTGGCCATT 
CCATATGACG AGTTTTTGCT ATGGGAAACG AACACTTTAA TGAAAGCGAA ATTGCGCGTC 
GGAACTCCCG CCAACGCTCC GGTGAAGAGA CCCAGAGAAT CAGACAATGA TTATGATGCA 
AATTCTTGGA AACAGATTAA ATCTTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)
Johnston M,Andrews S,Brinkman R,Cooper J,Ding H,Dover J,Du Z,Favello A,Fulton L,Gattung S

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PCL5 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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