GIC1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	GIC1
Entrez Gene ID	856458
Full Name	Gic1p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179191.1	NP_011928.1	Gic1p

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Johnston M, Andrews S, Brinkman R, Cooper J, Ding H, Dover J, Du Z, Favello A, Fulton L, Gattung S
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we performed domain analysis to identify domain(s) of Gic1 that are important for its boundary activity, and identified two minimum domains, which are located outside its Cdc42-binding domain.
Title: Gic1 is a novel heterochromatin boundary protein in vivo.

we have used electron microscopy and cryo electron tomography to elucidate the structural basis of the Gic1-septin and Gic1-Cdc42-septin interaction
Title: The role of Cdc42 and Gic1 in the regulation of septin filament formation and dissociation.

High-copy GIC1 suppresses the growth defect of a PKC1 mutant (stt1), suggesting the existence of a Pkc1-Zds1-Gic1 pathway.
Title: Pkc1 acts through Zds1 and Gic1 to suppress growth and cell polarity defects of a yeast eIF5A mutant.

The following GIC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GIC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi05259	NM_001179191.1 Latest version!	Saccharomyces cerevisiae S288C Gic1p (GIC1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GIC1 ( NM_001179191.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011928.1 Saccharomyces cerevisiae S288C Gic1p (GIC1), partial mRNA.

Price & Availability↑

CloneID	OSi05259
Clone ID Related Accession (Same CDS sequence)	NM_001179191.1
Accession Version	NM_001179191.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Gic1p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001140). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Veresion :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGACTGAAG GAAAGAGGCT GCAACAGATG GAGCTTCCTC AAATGAAATC CATTTGGATT 
GACGAGGATC AGGAAATGGA AAAATTGTAT GGATTCCAAG TAAGGCAACG ATTCATGAAT 
GGACCTAGTA CGGATTCCGA TGAAGACGCC GACGAAGATT TAGGAATTGT TCTCGTTGAC 
AGTAAAAAGC TGGCTTTGCC GAACAAGAAC AACATCAAAT TGCCCCCTTT GCCCAATTAC 
ATGACGATCA ACCCTAACAT AAATTCCAAT CACAAGTCAT TAACTAATAA AAAGAAGAAT 
TTCCTGGGCA TGTTCAAAAA AAAGGACCTG TTGTCGAGGA GACATGGGTC TGCCAAAACC 
GCAAAACAGT CAAGTATATC TACACCATTT GATTTTCACC ATATTTCGCA TGCTAATGGT 
AAAAGGGAAG ACAACCCTCT TGAGTCGCAC GAAGAAAAAC ATGATGTAGA ATCATTAGTC 
AAATTCACGT CTTTGGCACC GCAACCCCGA CCAGATTCAA ACGTCTCTTC TAAATATTCC 
AATGTTGTGA TGAACGATTC GAGCAGAATA GTGTCTTCCT CCACAATAGC TACAACGATG 
GATTCTCACC ACGATGGTAA CGAAACCAAC AATACCCCAA ATGGCAATAA GCAATTAGAC 
TCGCCTACAG ATTTGGAAAT GACCTTGGAA GACTTGAGAA ATTATACATT TCCTTCTGTT 
CTTGGAGATA GCGTCAGCGA AAAGACCAAT CCTTCCTCTC CCTCTGTTTC ATCATTTTCT 
GGCAAATTCA AGCCAAGAGA GTTGAGTGCG CTACATACGC CCGAATTAGG AAATTGTTTC 
AATGTAGATC AGTCGCTAAA TTCCCCTGGT AACAGAATAT CTGTGGATGA CGTGCTAAAA 
TTCTACTATC AATGTAGTGA AACTAGTACT CCTCGAAATA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011928.1
CDS	1..945
Translation	MTEGKRLQQMELPQMKSIWIDEDQEMEKLYGFQVRQRFMNGPSTDSDEDADEDLGIVLVDSKKLALPNKNNIKLPPLPNYMTINPNINSNHKSLTNKKKNFLGMFKKKDLLSRRHGSAKTAKQSSISTPFDFHHISHANGKREDNPLESHEEKHDVESLVKFTSLAPQPRPDSNVSSKYSNVVMNDSSRIVSSSTIATTMDSHHDGNETNNTPNGNKQLDSPTDLEMTLEDLRNYTFPSVLGDSVSEKTNPSSPSVSSFSGKFKPRELSALHTPELGNCFNVDQSLNSPGNRISVDDVLKFYYQCSETSTPRNT

Target ORF information:

RefSeq Version	NM_001179191.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Gic1p (GIC1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179191.1

ORF Insert Sequence:

ATGACTGAAG GAAAGAGGCT GCAACAGATG GAGCTTCCTC AAATGAAATC CATTTGGATT 
GACGAGGATC AGGAAATGGA AAAATTGTAT GGATTCCAAG TAAGGCAACG ATTCATGAAT 
GGACCTAGTA CGGATTCCGA TGAAGACGCC GACGAAGATT TAGGAATTGT TCTCGTTGAC 
AGTAAAAAGC TGGCTTTGCC GAACAAGAAC AACATCAAAT TGCCCCCTTT GCCCAATTAC 
ATGACGATCA ACCCTAACAT AAATTCCAAT CACAAGTCAT TAACTAATAA AAAGAAGAAT 
TTCCTGGGCA TGTTCAAAAA AAAGGACCTG TTGTCGAGGA GACATGGGTC TGCCAAAACC 
GCAAAACAGT CAAGTATATC TACACCATTT GATTTTCACC ATATTTCGCA TGCTAATGGT 
AAAAGGGAAG ACAACCCTCT TGAGTCGCAC GAAGAAAAAC ATGATGTAGA ATCATTAGTC 
AAATTCACGT CTTTGGCACC GCAACCCCGA CCAGATTCAA ACGTCTCTTC TAAATATTCC 
AATGTTGTGA TGAACGATTC GAGCAGAATA GTGTCTTCCT CCACAATAGC TACAACGATG 
GATTCTCACC ACGATGGTAA CGAAACCAAC AATACCCCAA ATGGCAATAA GCAATTAGAC 
TCGCCTACAG ATTTGGAAAT GACCTTGGAA GACTTGAGAA ATTATACATT TCCTTCTGTT 
CTTGGAGATA GCGTCAGCGA AAAGACCAAT CCTTCCTCTC CCTCTGTTTC ATCATTTTCT 
GGCAAATTCA AGCCAAGAGA GTTGAGTGCG CTACATACGC CCGAATTAGG AAATTGTTTC 
AATGTAGATC AGTCGCTAAA TTCCCCTGGT AACAGAATAT CTGTGGATGA CGTGCTAAAA 
TTCTACTATC AATGTAGTGA AACTAGTACT CCTCGAAATA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)
Johnston M,Andrews S,Brinkman R,Cooper J,Ding H,Dover J,Du Z,Favello A,Fulton L,Gattung S

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GIC1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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