STE20 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	STE20
Entrez Gene ID	856382
Full Name	mitogen-activated protein kinase kinase kinase kinase STE20
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179087.1	NP_011856.1	mitogen-activated protein kinase kinase kinase kinase STE20

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Pak1	NP_058894.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002986	XP_311901.5
Schizosaccharomyces pombe (fission yeast)	shk1	NP_596626.1
Pan troglodytes (chimpanzee)	PAK1	XP_508657.3
Saccharomyces cerevisiae (baker's yeast)	STE20	NP_011856.1
Homo sapiens (human)	PAK1	NP_002567.3
Caenorhabditis elegans (roundworm)	pak-1	NP_001024378.1
Canis lupus familiaris (dog)	PAK1	XP_849651.1
Bos taurus (cattle)	PAK1	NP_001070366.1
Danio rerio (zebrafish)	pak1	NP_958485.1
	PAK1	XP_001090310.1
Mus musculus (house mouse)	Pak1	NP_035165.2
Gallus gallus (chicken)	PAK1	NP_001155844.1
Drosophila melanogaster (fruit fly)	Pak	NP_001262313.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Johnston M, Andrews S, Brinkman R, Cooper J, Ding H, Dover J, Du Z, Favello A, Fulton L, Gattung S
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

during filamentous growth, Ste20 stimulates V-ATPase activity, this would sustain nutrient mobilization from vacuolar stores, which is beneficial for filamentous growth.
Title: Regulation of vacuolar H+-ATPase activity by the Cdc42 effector Ste20 in Saccharomyces cerevisiae.

Nbp2p SH3 domain made contacts with the Ste20 peptide core RXXPXXP motif and the Bem1p SH3b domain made stronger contacts with residues flanking the core motif.
Title: Distinct peptide binding specificities of Src homology 3 (SH3) protein domains can be determined by modulation of local energetics across the binding interface.

cell cycle kinases Ste20 as central mediators of the cellular response to inhibition of fatty acid synthesis. (Ste20)
Title: Global mapping of protein phosphorylation events identifies Ste20, Sch9 and the cell-cycle regulatory kinases Cdc28/Pho85 as mediators of fatty acid starvation responses in Saccharomyces cerevisiae.

The signaling proteins Ste5 and Ste20 have recognition sequences, or "docking" sites, for G1/S (interphase) cyclins. These docking sites bind preferentially to the cyclin Cln2, and strongly enhance Cln2-driven in vivo substrate phosphorylation.
Title: Cyclin-specific docking motifs promote phosphorylation of yeast signaling proteins by G1/S Cdk complexes.

Ste20 has an unexpected role in the modulation of mRNA decay and translation in Saccharomyces cerevisiae. Phosphorylation of Dcp2 by Ste20 is an important control point for mRNA decapping.
Title: Dcp2 phosphorylation by Ste20 modulates stress granule assembly and mRNA decay in Saccharomyces cerevisiae.

The following STE20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the STE20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi05190	NM_001179087.1 Latest version!	Saccharomyces cerevisiae S288C mitogen-activated protein kinase kinase kinase kinase STE20 (STE20), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

STE20 ( NM_001179087.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011856.1 Saccharomyces cerevisiae S288C mitogen-activated protein kinase kinase kinase kinase STE20 (STE20), partial mRNA.

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CloneID	OSi05190
Clone ID Related Accession (Same CDS sequence)	NM_001179087.1
Accession Version	NM_001179087.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2820bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	mitogen-activated protein kinase kinase kinase kinase STE20
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001140). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Veresion :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAGCAATG ATCCATCTGC TGTATCGGAA CTACCAGACA AGGACAGTCT TGATAACGGT 
ATCAGCAATG ACAATGAAAG GGCCATGGGC GGCAATGGCG ATGGCGGCGA TGGATTACGA 
TTACCAAGGA CCACTGGAAC TTTGAACGTC AATGCCTTAC AAAAAGGCAC TAATGCTGCC 
CATGAAGCTG GTGGATACAA ATCCATGGAT CCTGCGAAGA ACGCGGAGAC AACCAATGAT 
GATGACAATA ATGTCGTTTC ACTAGATGAT CCTATTCAAT TTACCCGAGT ATCTTCCTCC 
TCTGTCATCA GTGGAATGTC TTCATCCATG AGTCCTCATT CTAACATCGA TGAAACCAAA 
TCTCTAGAAG CAGTCACTCC AAACATAAAT ACCAGCAATA TAACCCCGGA TCATTCTGCT 
GACAACACAT TTTCTACCAT AAATGCGTCC GAGTCAGATC ACCAGTTTAA TGACACTTTA 
CTATCAAAAC TGTCGTTAAC AGATTCTACA GAAACTATAG AAAATAACGC GACAGTGAAG 
CACCAGCAGC CAGTTGCATC TTCCACAGTA AACTCGAATA AGAGCTCCAC TGATATACGA 
AGGGCTACAC CAGTGTCCAC TCCCGTTATC TCTAAACCAT CGATGACAAC CACGCCAAGA 
CAGATCAATT CAGCTTCCCA TTCGCTTTCG AACCCTAAGC ATAAGCAACA TAAACCAAAA 
GTTAAACCGT CCAAGCCTGA AGCAAAAAGT AAACCGGTTT CTGTGAAAAA AAGCTTTCCT 
TCGAAAAATC CTTTAAAAAA CTCCTCTCCA CCTAAAAAGC AAACAGAAAA ATCGTATTAT 
TCTTCCTCTT CGAAAAAAAG GAAAAGCGGT TCAAATAGTG GTACACTAAG AATGAAAGAT 
GTCTTTACGT CCTTTGTACA GAATATAAAG AGAAATTCTC AGGATGATAA AAGGGCCTCA 
TCGTCGTCCA ATAATTCTTC CTCATCTTCT ATAACCACCG CTTTGAGGAT ATCTACGCCA 
TACAATGCCA AGCATATCCA CCATGTGGGC GTGGACTCCA AGACTGGTGA GTACACAGGT 
TTGCCGGAGG AATGGGAAAA ATTGTTGACT TCTAGTGGTA TTTCCAAAAG AGAACAACAG 
CAAAACATGC AAGCAGTCAT GGATATTGTC AAATTCTATC AGGATGTCAC GGAAACAAAC 
GGTGAAGATA AAATGTTCAA GACTTTCAAC ACAACCACAG GATTGCCGGG AAGTCCTCAA 
GTTTCAACAC CGCCTGCAAA CTCATTCAAT AAATTTCCTC CGTCGACAAG TGATTCGCAC 
AATTACGGTT CCAGAACAGG TACACCAATG TCCAATCACG TCATGTCTCC AACCTTAAAT 
ACAGATTCTA GTTCAGCAAA CGGGAAATTC ATACCAAGTA GACCGGCTCC TAAGCCCCCA 
TCTTCTGCGT CCGCTTCAGC TCCAATTATA AAATCACCCG TCATGAATTC TGCCGCCAAT 
GTTTCGCCCT TGAAGCAGAC TCATGCACCT ACAACTCCGA ACAGGACCAG CCCAAACAGG 
TCCTCAATAT CAAGAAATGC CACTTTAAAA AAAGAGGAGC AGCCACTACC ACCAATACCT 
CCAACCAAAT CCAAAACGTC TCCAATCATC TCCACAGCTC ACACACCACA GCAAGTTGCT 
CAATCGCCAA AAGCGCCGGC GCAAGAGACG GTAACGACAC CTACTTCGAA GCCAGCTCAA 
GCAAGAAGCT TGTCTAAAGA ATTAAATGAG AAAAAGAGAG AGGAAAGGGA AAGACGTAAA 
AAACAACTAT ATGCCAAATT GAACGAAATT TGCTCAGACG GTGACCCAAG TACAAAATAT 
GCCAATTTAG TAAAAATTGG TCAAGGTGCA TCAGGTGGTG TTTATACTGC TTATGAAATA 
GGTACGAATG TCTCAGTGGC CATTAAGCAA ATGAATCTCG AAAAGCAACC AAAAAAGGAG 
CTAATCATCA ATGAGATTCT GGTCATGAAG GGTAGCAAAC ACCCTAATAT AGTTAATTTC 
ATTGATTCTT ACGTTTTAAA AGGCGACCTT TGGGTCATTA TGGAATACAT GGAAGGTGGC 
TCCTTAACTG ATGTGGTCAC CCATTGTATT TTGACAGAAG GTCAAATTGG TGCCGTTTGT 
AGAGAAACTT TGAGTGGGTT GGAATTTTTA CATTCTAAAG GTGTTCTTCA CAGAGATATC 
AAATCCGATA ACATCCTATT GTCCATGGAA GGGGATATTA AGTTAACGGA TTTCGGTTTT 
TGCGCTCAAA TCAATGAATT GAACTTGAAA AGAACTACTA TGGTGGGAAC GCCTTATTGG 
ATGGCGCCTG AAGTGGTTTC TAGGAAAGAA TATGGCCCAA AAGTAGATAT CTGGTCGTTG 
GGTATCATGA TCATTGAAAT GATCGAGGGG GAGCCTCCAT ATTTAAATGA AACCCCGCTA 
AGAGCACTGT ATTTAATTGC TACAAATGGT ACACCCAAGT TAAAGGAACC CGAGAATCTA 
TCGTCAAGCT TGAAAAAATT CCTTGATTGG TGTTTATGTG TGGAGCCCGA AGATAGAGCA 
AGCGCTACGG AATTGCTTCA TGATGAATAT ATCACGGAGA TAGCTGAAGC CAATTCCTCA 
TTGGCCCCGC TAGTCAAGTT AGCAAGATTG AAGAAAGTAG CTGAGAACAT GGATGCTGAT 
GAAGATAATG ACGACGATAA CGACAACGAG CATATTAATA AGACAAACAA TTGTGACGAC 
AATAACGATA GCAAAGAAAC CGTAAATTTG GACGTAACTG AAGATGATAA ACAAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011856.1
CDS	1..2820
Translation	MSNDPSAVSELPDKDSLDNGISNDNERAMGGNGDGGDGLRLPRTTGTLNVNALQKGTNAAHEAGGYKSMDPAKNAETTNDDDNNVVSLDDPIQFTRVSSSSVISGMSSSMSPHSNIDETKSLEAVTPNINTSNITPDHSADNTFSTINASESDHQFNDTLLSKLSLTDSTETIENNATVKHQQPVASSTVNSNKSSTDIRRATPVSTPVISKPSMTTTPRQINSASHSLSNPKHKQHKPKVKPSKPEAKSKPVSVKKSFPSKNPLKNSSPPKKQTEKSYYSSSSKKRKSGSNSGTLRMKDVFTSFVQNIKRNSQDDKRASSSSNNSSSSSITTALRISTPYNAKHIHHVGVDSKTGEYTGLPEEWEKLLTSSGISKREQQQNMQAVMDIVKFYQDVTETNGEDKMFKTFNTTTGLPGSPQVSTPPANSFNKFPPSTSDSHNYGSRTGTPMSNHVMSPTLNTDSSSANGKFIPSRPAPKPPSSASASAPIIKSPVMNSAANVSPLKQTHAPTTPNRTSPNRSSISRNATLKKEEQPLPPIPPTKSKTSPIISTAHTPQQVAQSPKAPAQETVTTPTSKPAQARSLSKELNEKKREERERRKKQLYAKLNEICSDGDPSTKYANLVKIGQGASGGVYTAYEIGTNVSVAIKQMNLEKQPKKELIINEILVMKGSKHPNIVNFIDSYVLKGDLWVIMEYMEGGSLTDVVTHCILTEGQIGAVCRETLSGLEFLHSKGVLHRDIKSDNILLSMEGDIKLTDFGFCAQINELNLKRTTMVGTPYWMAPEVVSRKEYGPKVDIWSLGIMIIEMIEGEPPYLNETPLRALYLIATNGTPKLKEPENLSSSLKKFLDWCLCVEPEDRASATELLHDEYITEIAEANSSLAPLVKLARLKKVAENMDADEDNDDDNDNEHINKTNNCDDNNDSKETVNLDVTEDDKQK

Target ORF information:

RefSeq Version	NM_001179087.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C mitogen-activated protein kinase kinase kinase kinase STE20 (STE20), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179087.1

ORF Insert Sequence:

ATGAGCAATG ATCCATCTGC TGTATCGGAA CTACCAGACA AGGACAGTCT TGATAACGGT 
ATCAGCAATG ACAATGAAAG GGCCATGGGC GGCAATGGCG ATGGCGGCGA TGGATTACGA 
TTACCAAGGA CCACTGGAAC TTTGAACGTC AATGCCTTAC AAAAAGGCAC TAATGCTGCC 
CATGAAGCTG GTGGATACAA ATCCATGGAT CCTGCGAAGA ACGCGGAGAC AACCAATGAT 
GATGACAATA ATGTCGTTTC ACTAGATGAT CCTATTCAAT TTACCCGAGT ATCTTCCTCC 
TCTGTCATCA GTGGAATGTC TTCATCCATG AGTCCTCATT CTAACATCGA TGAAACCAAA 
TCTCTAGAAG CAGTCACTCC AAACATAAAT ACCAGCAATA TAACCCCGGA TCATTCTGCT 
GACAACACAT TTTCTACCAT AAATGCGTCC GAGTCAGATC ACCAGTTTAA TGACACTTTA 
CTATCAAAAC TGTCGTTAAC AGATTCTACA GAAACTATAG AAAATAACGC GACAGTGAAG 
CACCAGCAGC CAGTTGCATC TTCCACAGTA AACTCGAATA AGAGCTCCAC TGATATACGA 
AGGGCTACAC CAGTGTCCAC TCCCGTTATC TCTAAACCAT CGATGACAAC CACGCCAAGA 
CAGATCAATT CAGCTTCCCA TTCGCTTTCG AACCCTAAGC ATAAGCAACA TAAACCAAAA 
GTTAAACCGT CCAAGCCTGA AGCAAAAAGT AAACCGGTTT CTGTGAAAAA AAGCTTTCCT 
TCGAAAAATC CTTTAAAAAA CTCCTCTCCA CCTAAAAAGC AAACAGAAAA ATCGTATTAT 
TCTTCCTCTT CGAAAAAAAG GAAAAGCGGT TCAAATAGTG GTACACTAAG AATGAAAGAT 
GTCTTTACGT CCTTTGTACA GAATATAAAG AGAAATTCTC AGGATGATAA AAGGGCCTCA 
TCGTCGTCCA ATAATTCTTC CTCATCTTCT ATAACCACCG CTTTGAGGAT ATCTACGCCA 
TACAATGCCA AGCATATCCA CCATGTGGGC GTGGACTCCA AGACTGGTGA GTACACAGGT 
TTGCCGGAGG AATGGGAAAA ATTGTTGACT TCTAGTGGTA TTTCCAAAAG AGAACAACAG 
CAAAACATGC AAGCAGTCAT GGATATTGTC AAATTCTATC AGGATGTCAC GGAAACAAAC 
GGTGAAGATA AAATGTTCAA GACTTTCAAC ACAACCACAG GATTGCCGGG AAGTCCTCAA 
GTTTCAACAC CGCCTGCAAA CTCATTCAAT AAATTTCCTC CGTCGACAAG TGATTCGCAC 
AATTACGGTT CCAGAACAGG TACACCAATG TCCAATCACG TCATGTCTCC AACCTTAAAT 
ACAGATTCTA GTTCAGCAAA CGGGAAATTC ATACCAAGTA GACCGGCTCC TAAGCCCCCA 
TCTTCTGCGT CCGCTTCAGC TCCAATTATA AAATCACCCG TCATGAATTC TGCCGCCAAT 
GTTTCGCCCT TGAAGCAGAC TCATGCACCT ACAACTCCGA ACAGGACCAG CCCAAACAGG 
TCCTCAATAT CAAGAAATGC CACTTTAAAA AAAGAGGAGC AGCCACTACC ACCAATACCT 
CCAACCAAAT CCAAAACGTC TCCAATCATC TCCACAGCTC ACACACCACA GCAAGTTGCT 
CAATCGCCAA AAGCGCCGGC GCAAGAGACG GTAACGACAC CTACTTCGAA GCCAGCTCAA 
GCAAGAAGCT TGTCTAAAGA ATTAAATGAG AAAAAGAGAG AGGAAAGGGA AAGACGTAAA 
AAACAACTAT ATGCCAAATT GAACGAAATT TGCTCAGACG GTGACCCAAG TACAAAATAT 
GCCAATTTAG TAAAAATTGG TCAAGGTGCA TCAGGTGGTG TTTATACTGC TTATGAAATA 
GGTACGAATG TCTCAGTGGC CATTAAGCAA ATGAATCTCG AAAAGCAACC AAAAAAGGAG 
CTAATCATCA ATGAGATTCT GGTCATGAAG GGTAGCAAAC ACCCTAATAT AGTTAATTTC 
ATTGATTCTT ACGTTTTAAA AGGCGACCTT TGGGTCATTA TGGAATACAT GGAAGGTGGC 
TCCTTAACTG ATGTGGTCAC CCATTGTATT TTGACAGAAG GTCAAATTGG TGCCGTTTGT 
AGAGAAACTT TGAGTGGGTT GGAATTTTTA CATTCTAAAG GTGTTCTTCA CAGAGATATC 
AAATCCGATA ACATCCTATT GTCCATGGAA GGGGATATTA AGTTAACGGA TTTCGGTTTT 
TGCGCTCAAA TCAATGAATT GAACTTGAAA AGAACTACTA TGGTGGGAAC GCCTTATTGG 
ATGGCGCCTG AAGTGGTTTC TAGGAAAGAA TATGGCCCAA AAGTAGATAT CTGGTCGTTG 
GGTATCATGA TCATTGAAAT GATCGAGGGG GAGCCTCCAT ATTTAAATGA AACCCCGCTA 
AGAGCACTGT ATTTAATTGC TACAAATGGT ACACCCAAGT TAAAGGAACC CGAGAATCTA 
TCGTCAAGCT TGAAAAAATT CCTTGATTGG TGTTTATGTG TGGAGCCCGA AGATAGAGCA 
AGCGCTACGG AATTGCTTCA TGATGAATAT ATCACGGAGA TAGCTGAAGC CAATTCCTCA 
TTGGCCCCGC TAGTCAAGTT AGCAAGATTG AAGAAAGTAG CTGAGAACAT GGATGCTGAT 
GAAGATAATG ACGACGATAA CGACAACGAG CATATTAATA AGACAAACAA TTGTGACGAC 
AATAACGATA GCAAAGAAAC CGTAAATTTG GACGTAACTG AAGATGATAA ACAAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)
Johnston M,Andrews S,Brinkman R,Cooper J,Ding H,Dover J,Du Z,Favello A,Fulton L,Gattung S

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STE20 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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