NPR3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	NPR3
Entrez Gene ID	856362
Full Name	Npr3p
Synonyms	RMD11
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179103.1	NP_011840.1	Npr3p

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	NPR3	NP_011840.1
Kluyveromyces lactis	KLLA0F18238g	XP_455898.1
Eremothecium gossypii	AGOS_AGL039W	NP_986627.2

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Johnston M, Andrews S, Brinkman R, Cooper J, Ding H, Dover J, Du Z, Favello A, Fulton L, Gattung S
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Npr2-Npr3-dependent GTP hydrolysis of Gtr1 and direct binding of Gtr2 to Kog1, are involved in TORC1 inactivation and autophagic induction
Title: Reciprocal conversion of Gtr1 and Gtr2 nucleotide-binding states by Npr2-Npr3 inactivates TORC1 and induces autophagy.

A visual screen uncovered three regulators of autophagy-Iml1p, Npr2p, and Npr3p-which function in the same complex and are selectively required for non-nitrogen starvation-induced autophagy
Title: Selective regulation of autophagy by the Iml1-Npr2-Npr3 complex in the absence of nitrogen starvation.

Npr2/3 complex mediates an amino acid starvation signal to TORC1.
Title: A genome-wide screen for regulators of TORC1 in response to amino acid starvation reveals a conserved Npr2/3 complex.

The following NPR3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NPR3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi05172	NM_001179103.1 Latest version!	Saccharomyces cerevisiae S288C Npr3p (NPR3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NPR3 ( NM_001179103.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011840.1 Saccharomyces cerevisiae S288C Npr3p (NPR3), partial mRNA.

Price & Availability↑

CloneID	OSi05172
Clone ID Related Accession (Same CDS sequence)	NM_001179103.1
Accession Version	NM_001179103.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Npr3p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001140). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Veresion :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGATGAAT GTTTACCCAA TTCATGTCTA CTAGGTGTTC ACCTCGTCAT TTCAACGCAT 
TCGGGGCCAC AAATAGTTTA TCACTACCCA CCCTCCAATA CAGCATTTCT AACAAATAAT 
CCAACGAAGC ATCAGCACCT CTATGGAAAC CATGCAAATC TAAATAAAAA CACAAGTACA 
AATAAAGAAG AAAAACTCTT TAATTCTGGC TCTACGAAAA CAGCTTCTCA AATTGCTCTA 
AACGAGTCAG CGAAAAGTTA CAATACCGCT ATTACGCCAT CAATGACAAA TACTAATACT 
AATAATGTGA CATTACCTCC AACTAGATCG CATGCTAATA CTGTAGGATC ACAAAGTTCA 
ATTCCTGCGG CTACTAATGG TGTAGGTTAT AGAAAAACCG ATATCGAAGA TACTTCTCGG 
ACTTTTCAAT ACCAAGAAAC TGAATCTGAA ACGTCATCAT CGGGATTAAG TGACAGCGAA 
TTGTCCACAG ACTACTTAGA CATTTCTAGC GATTCGTTTT CTATATCTTC TTCCTTATCG 
TCATCTTCTT TATCTTCTTC TCCCTCAAGT TCATCCTCAT CTTCCCCTCC GCAAGATGGC 
TTATCAAGAA CTAATTCGAG TTTCCAATCT ACGGATTCAA TGTCTCCAAC TTCACCACAA 
ATGATAATGG AAAACGATAG TATTTCAGTA GCGGAATCAT ATCTAGATTC TGGGACTAAT 
AACAAATCAA GGGCCGCTTC CAAGAGATCC CAGAATTTCT TCCATAAATT GAGTACCAAG 
AAGAGTACTG ATTCAAAAAC ACATTCTCCA GTAAGAAAGC TTAAATCTAA GCCATCACAA 
AGCACTAAAA AAGGCAACAA ACTGTTAAAA AATACGTCCA ATGAGACTGA TGGAAATGCT 
TTTACAGGAA GTTGTTCAAT TTCTTCTAAA AAATCTTTAT CGAGTACAGG AGAGCATAAC 
CAAGAACTTC GTAATAGTAG CCTGAACGAC ACTCCTGGCC AATCACCTCA TCATTACCAC 
CATCGTTACC ACCATTATCA TAAAAATGCC GCGACGAGCC AACGAAATTC CCATACCCAG 
TACGATGTTG AAGAAGAAGA CATGGAAGTT TCGGCAATGT TGCAGGATGG TAAAATCTCT 
ATGAATGAAA TTTTTTTTGA AGAGGAAAAC TTCCAGGATA TAAATAAAAT TCTAGAATTT 
GATAATGATT TTGTTGCAGA ATTTTGTAGC CCTGAGAGGG AAATGTGCAA TACCAGATTC 
GAATTCACGG TGGATAATTT CTGCTTCTTA GGCTTACCTA TTCATGTAGA TTCTCAAGGC 
AGATGGAGAA AATCCAAGCA CAAAAATAAA ACCCGCTCTA AAAGATCTTC GAGTACTACA 
ACAAACATCA GTAGAAAGAA ATCTATCGCT TCCAAAATTT CATCACTGAG TGAGAATACT 
TTGAAGAAAG TTAATAGTGG TGAAGCTGAC ACTGTTTATG ACAGTAATAT TGGTCATGAG 
GCTTCTACTG ATACGCCGAA TTTGAGGATA AATACTGATG TCAGTGGAAA CGAATTTGAG 
AGAGAAAAGG AAGACTTGGG CAAAAATATG AACATGTTTC ATGTATGCTT TGTTATGAAT 
CCGCATTTAA TCGAATATAA TAAAAGGATT GATGACATGT ATCAGTTTGT TGTTACAAGG 
TTATCATTAT TGTTAAGATA TGTGCAATCA AAAACCTCGT ACATTTCTAG CGAATGCCAT 
ATAATTTTAA AGGAAAAAGA ACGGGTACTG AAACATTCGA AAACATACCA ATCTATAAGA 
GGCGCAGGAA ACAAGGGGAA GTATCTGTAC CAAAGAATTT TAGCCAAATC TTCGTTGGCT 
AGGGCGCTAA CGGAATGTGT TGATAAAATC CAACGAAATG AAATCGCATG TCTTGAGATC 
AATGACGATA AAGTAATTTC CTTGCAAATC CCGATTCAAA ACGAGTTTGA AAAAATGCCA 
AACTTCAAAC TGCAACCCGT GCTAAGAGGC TCATATTTGA CGTCCATTTT GAACATGAAA 
TTTTTAGAAA AATCATCATT GAGAATTGAA AGTCAAAATC GTCAAAACGA TCAGGCTCAA 
TTCAGTGATA CCAATAATAA CATATACAGG TTTGGCAATA ATATTAATAG TACCGGCCAC 
TGTGGAGCAG CAAATGTGGA TGACGGGGAC GACAATGAAA GCAATTACTA CTGCGACGAC 
AATGATGACC TTTTAAACTA TGCGCTATTA TTATTGGACG AGCCAAATAA TATTATTAGC 
TCCCTCGAAA CTTTTTCATA CCAGGACGAT ATTGGCACTA TCATATTGAA ACATTTAGTC 
AGAAATATCC AGCCAAATAT CCCTCTAAGG TCGTATCGCT ATCTGATAAC TGATTTATTA 
GATAACCCTT CTTCACTGGA CGACCTTACT ACAGAAACAA ACTCATTGGA ATCAAGCATA 
CTGCGCTCTT GCGCATTGCA TTTAATGTAT TGGAGACATG CAAGAATAGT GATCCCACTA 
AGCTCCAAAT ACACGTATAT TGTTTCACCC TTGGCTCCTA TTCAAGGTTA TACAATAGAT 
GATTATAAAA GTACTTCACA AAATGATGGG AACGTGAAAA AAATGGACGA TCGCGAAAAT 
AATAAGAGTG GAAGCGACAG AGTACCACTA ATTTATCAAA ATTCGATGTT GTTCAGATCC 
AAATTCCCAT CATTGCCCAG TTTACCCATT TTTTTAAGTT TGTTGTCCAC GGACAAACCT 
CAAGCGTACA GTAACATAAT CCCATCAAGG GAACATAAGC CAGTTTACCT GAACGCGTTA 
GCGTGGTTAA TACAGTACGG GTATGTAACC CAATTATTGA CTTTCATAAA TATCCGAGTG 
GACAAACACA TAAAAATGGC AGTAGATGAG GATCTAGAAA AGGAAGGCTT CCGTAAGACT 
AATACTGCTA GAAGGCCCAG CATGGACTAC AAAAAAACTG ATAAAAAGCT GGATGATGAA 
GATGGGCAAA GTAGAGATGC AAACGCCAGC GAAGCGTGTT CAGGAAAGAA TGAAGGCATG 
CAGTCCAACG ACAACAACAA AGATGTGGAC GAAAAAGATA ACGAAAATGA TAGTCGTGTT 
GATGATCGTG ATGACAATGA AATAGCTATT GCGGATGAAG AAGAAATACT ACACTTCGAA 
TACGATGATC CAGAAATGCA ACATGACTAT ACTATAATTT TGGAACCTGA AAGAGCCACT 
GCGATTGAAA AAAGATGGCT TTATAGATGT ATTTATGGTC AGCCTTCAGA TATCCAGATT 
CTTTTTAACA AGTTGCTCAA GTACTTCAAT GGAAAGGTCC CCATGGAATT GGTTATCATC 
AAAGAGGAAA TTAGCAGACA TGACCTAAAG AAATTGCTCA ATGCTCTAGA TAAGTATTTA 
ATCGAGATAC ACCATTGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011840.1
CDS	1..3441
Translation	MDECLPNSCLLGVHLVISTHSGPQIVYHYPPSNTAFLTNNPTKHQHLYGNHANLNKNTSTNKEEKLFNSGSTKTASQIALNESAKSYNTAITPSMTNTNTNNVTLPPTRSHANTVGSQSSIPAATNGVGYRKTDIEDTSRTFQYQETESETSSSGLSDSELSTDYLDISSDSFSISSSLSSSSLSSSPSSSSSSSPPQDGLSRTNSSFQSTDSMSPTSPQMIMENDSISVAESYLDSGTNNKSRAASKRSQNFFHKLSTKKSTDSKTHSPVRKLKSKPSQSTKKGNKLLKNTSNETDGNAFTGSCSISSKKSLSSTGEHNQELRNSSLNDTPGQSPHHYHHRYHHYHKNAATSQRNSHTQYDVEEEDMEVSAMLQDGKISMNEIFFEEENFQDINKILEFDNDFVAEFCSPEREMCNTRFEFTVDNFCFLGLPIHVDSQGRWRKSKHKNKTRSKRSSSTTTNISRKKSIASKISSLSENTLKKVNSGEADTVYDSNIGHEASTDTPNLRINTDVSGNEFEREKEDLGKNMNMFHVCFVMNPHLIEYNKRIDDMYQFVVTRLSLLLRYVQSKTSYISSECHIILKEKERVLKHSKTYQSIRGAGNKGKYLYQRILAKSSLARALTECVDKIQRNEIACLEINDDKVISLQIPIQNEFEKMPNFKLQPVLRGSYLTSILNMKFLEKSSLRIESQNRQNDQAQFSDTNNNIYRFGNNINSTGHCGAANVDDGDDNESNYYCDDNDDLLNYALLLLDEPNNIISSLETFSYQDDIGTIILKHLVRNIQPNIPLRSYRYLITDLLDNPSSLDDLTTETNSLESSILRSCALHLMYWRHARIVIPLSSKYTYIVSPLAPIQGYTIDDYKSTSQNDGNVKKMDDRENNKSGSDRVPLIYQNSMLFRSKFPSLPSLPIFLSLLSTDKPQAYSNIIPSREHKPVYLNALAWLIQYGYVTQLLTFINIRVDKHIKMAVDEDLEKEGFRKTNTARRPSMDYKKTDKKLDDEDGQSRDANASEACSGKNEGMQSNDNNKDVDEKDNENDSRVDDRDDNEIAIADEEEILHFEYDDPEMQHDYTIILEPERATAIEKRWLYRCIYGQPSDIQILFNKLLKYFNGKVPMELVIIKEEISRHDLKKLLNALDKYLIEIHHW

Target ORF information:

RefSeq Version	NM_001179103.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Npr3p (NPR3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179103.1

ORF Insert Sequence:

ATGGATGAAT GTTTACCCAA TTCATGTCTA CTAGGTGTTC ACCTCGTCAT TTCAACGCAT 
TCGGGGCCAC AAATAGTTTA TCACTACCCA CCCTCCAATA CAGCATTTCT AACAAATAAT 
CCAACGAAGC ATCAGCACCT CTATGGAAAC CATGCAAATC TAAATAAAAA CACAAGTACA 
AATAAAGAAG AAAAACTCTT TAATTCTGGC TCTACGAAAA CAGCTTCTCA AATTGCTCTA 
AACGAGTCAG CGAAAAGTTA CAATACCGCT ATTACGCCAT CAATGACAAA TACTAATACT 
AATAATGTGA CATTACCTCC AACTAGATCG CATGCTAATA CTGTAGGATC ACAAAGTTCA 
ATTCCTGCGG CTACTAATGG TGTAGGTTAT AGAAAAACCG ATATCGAAGA TACTTCTCGG 
ACTTTTCAAT ACCAAGAAAC TGAATCTGAA ACGTCATCAT CGGGATTAAG TGACAGCGAA 
TTGTCCACAG ACTACTTAGA CATTTCTAGC GATTCGTTTT CTATATCTTC TTCCTTATCG 
TCATCTTCTT TATCTTCTTC TCCCTCAAGT TCATCCTCAT CTTCCCCTCC GCAAGATGGC 
TTATCAAGAA CTAATTCGAG TTTCCAATCT ACGGATTCAA TGTCTCCAAC TTCACCACAA 
ATGATAATGG AAAACGATAG TATTTCAGTA GCGGAATCAT ATCTAGATTC TGGGACTAAT 
AACAAATCAA GGGCCGCTTC CAAGAGATCC CAGAATTTCT TCCATAAATT GAGTACCAAG 
AAGAGTACTG ATTCAAAAAC ACATTCTCCA GTAAGAAAGC TTAAATCTAA GCCATCACAA 
AGCACTAAAA AAGGCAACAA ACTGTTAAAA AATACGTCCA ATGAGACTGA TGGAAATGCT 
TTTACAGGAA GTTGTTCAAT TTCTTCTAAA AAATCTTTAT CGAGTACAGG AGAGCATAAC 
CAAGAACTTC GTAATAGTAG CCTGAACGAC ACTCCTGGCC AATCACCTCA TCATTACCAC 
CATCGTTACC ACCATTATCA TAAAAATGCC GCGACGAGCC AACGAAATTC CCATACCCAG 
TACGATGTTG AAGAAGAAGA CATGGAAGTT TCGGCAATGT TGCAGGATGG TAAAATCTCT 
ATGAATGAAA TTTTTTTTGA AGAGGAAAAC TTCCAGGATA TAAATAAAAT TCTAGAATTT 
GATAATGATT TTGTTGCAGA ATTTTGTAGC CCTGAGAGGG AAATGTGCAA TACCAGATTC 
GAATTCACGG TGGATAATTT CTGCTTCTTA GGCTTACCTA TTCATGTAGA TTCTCAAGGC 
AGATGGAGAA AATCCAAGCA CAAAAATAAA ACCCGCTCTA AAAGATCTTC GAGTACTACA 
ACAAACATCA GTAGAAAGAA ATCTATCGCT TCCAAAATTT CATCACTGAG TGAGAATACT 
TTGAAGAAAG TTAATAGTGG TGAAGCTGAC ACTGTTTATG ACAGTAATAT TGGTCATGAG 
GCTTCTACTG ATACGCCGAA TTTGAGGATA AATACTGATG TCAGTGGAAA CGAATTTGAG 
AGAGAAAAGG AAGACTTGGG CAAAAATATG AACATGTTTC ATGTATGCTT TGTTATGAAT 
CCGCATTTAA TCGAATATAA TAAAAGGATT GATGACATGT ATCAGTTTGT TGTTACAAGG 
TTATCATTAT TGTTAAGATA TGTGCAATCA AAAACCTCGT ACATTTCTAG CGAATGCCAT 
ATAATTTTAA AGGAAAAAGA ACGGGTACTG AAACATTCGA AAACATACCA ATCTATAAGA 
GGCGCAGGAA ACAAGGGGAA GTATCTGTAC CAAAGAATTT TAGCCAAATC TTCGTTGGCT 
AGGGCGCTAA CGGAATGTGT TGATAAAATC CAACGAAATG AAATCGCATG TCTTGAGATC 
AATGACGATA AAGTAATTTC CTTGCAAATC CCGATTCAAA ACGAGTTTGA AAAAATGCCA 
AACTTCAAAC TGCAACCCGT GCTAAGAGGC TCATATTTGA CGTCCATTTT GAACATGAAA 
TTTTTAGAAA AATCATCATT GAGAATTGAA AGTCAAAATC GTCAAAACGA TCAGGCTCAA 
TTCAGTGATA CCAATAATAA CATATACAGG TTTGGCAATA ATATTAATAG TACCGGCCAC 
TGTGGAGCAG CAAATGTGGA TGACGGGGAC GACAATGAAA GCAATTACTA CTGCGACGAC 
AATGATGACC TTTTAAACTA TGCGCTATTA TTATTGGACG AGCCAAATAA TATTATTAGC 
TCCCTCGAAA CTTTTTCATA CCAGGACGAT ATTGGCACTA TCATATTGAA ACATTTAGTC 
AGAAATATCC AGCCAAATAT CCCTCTAAGG TCGTATCGCT ATCTGATAAC TGATTTATTA 
GATAACCCTT CTTCACTGGA CGACCTTACT ACAGAAACAA ACTCATTGGA ATCAAGCATA 
CTGCGCTCTT GCGCATTGCA TTTAATGTAT TGGAGACATG CAAGAATAGT GATCCCACTA 
AGCTCCAAAT ACACGTATAT TGTTTCACCC TTGGCTCCTA TTCAAGGTTA TACAATAGAT 
GATTATAAAA GTACTTCACA AAATGATGGG AACGTGAAAA AAATGGACGA TCGCGAAAAT 
AATAAGAGTG GAAGCGACAG AGTACCACTA ATTTATCAAA ATTCGATGTT GTTCAGATCC 
AAATTCCCAT CATTGCCCAG TTTACCCATT TTTTTAAGTT TGTTGTCCAC GGACAAACCT 
CAAGCGTACA GTAACATAAT CCCATCAAGG GAACATAAGC CAGTTTACCT GAACGCGTTA 
GCGTGGTTAA TACAGTACGG GTATGTAACC CAATTATTGA CTTTCATAAA TATCCGAGTG 
GACAAACACA TAAAAATGGC AGTAGATGAG GATCTAGAAA AGGAAGGCTT CCGTAAGACT 
AATACTGCTA GAAGGCCCAG CATGGACTAC AAAAAAACTG ATAAAAAGCT GGATGATGAA 
GATGGGCAAA GTAGAGATGC AAACGCCAGC GAAGCGTGTT CAGGAAAGAA TGAAGGCATG 
CAGTCCAACG ACAACAACAA AGATGTGGAC GAAAAAGATA ACGAAAATGA TAGTCGTGTT 
GATGATCGTG ATGACAATGA AATAGCTATT GCGGATGAAG AAGAAATACT ACACTTCGAA 
TACGATGATC CAGAAATGCA ACATGACTAT ACTATAATTT TGGAACCTGA AAGAGCCACT 
GCGATTGAAA AAAGATGGCT TTATAGATGT ATTTATGGTC AGCCTTCAGA TATCCAGATT 
CTTTTTAACA AGTTGCTCAA GTACTTCAAT GGAAAGGTCC CCATGGAATT GGTTATCATC 
AAAGAGGAAA TTAGCAGACA TGACCTAAAG AAATTGCTCA ATGCTCTAGA TAAGTATTTA 
ATCGAGATAC ACCATTGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)
Johnston M,Andrews S,Brinkman R,Cooper J,Ding H,Dover J,Du Z,Favello A,Fulton L,Gattung S

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NPR3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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