RIM4 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	RIM4
Entrez Gene ID	856361
Full Name	Rim4p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179104.1	NP_011839.1	Rim4p

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	RIM4	NP_011839.1
Eremothecium gossypii	AGOS_AGL038C	NP_986628.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Johnston M, Andrews S, Brinkman R, Cooper J, Ding H, Dover J, Du Z, Favello A, Fulton L, Gattung S
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Rim4 forms amyloid-like aggregates and that it is the amyloid-like form of Rim4 that is the active, translationally repressive form of the protein; data further show that Rim4 aggregation is a developmentally regulated process; at the onset of meiosis II, Rim4 aggregates are abruptly degraded allowing translation to commence.
Title: Regulated Formation of an Amyloid-like Translational Repressor Governs Gametogenesis.

The following RIM4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RIM4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi05171	NM_001179104.1 Latest version!	Saccharomyces cerevisiae S288C Rim4p (RIM4), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RIM4 ( NM_001179104.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011839.1 Saccharomyces cerevisiae S288C Rim4p (RIM4), partial mRNA.

Price & Availability↑

CloneID	OSi05171
Clone ID Related Accession (Same CDS sequence)	NM_001179104.1
Accession Version	NM_001179104.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2142bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Rim4p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001140). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Veresion :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAAAACCG AAATCAGCAC TGCGGATTCC CTTCGTGATC CTCCCTCTAA TGGTCTAAAG 
GCCGACTCTG AGCTGGTTAT CAGAGAGGAT ATAGACCAAT TTTTGCCTTC GGAAGTATCT 
TCGTTGGGGT CAGATCACCA GAATGATGGT GAGGATTCAG ACACTGACAG TGACAACTTT 
TTGCAAGACC CTGAAGACGA TGTGGATGAA GAAAGCACTG GTAGAGGTAC AGTCACTACC 
ACTTCCACAT CCACTGAGTC AAGAGGCCGT CCATCTTCTT GTATCTTCGT GGCAAGCTTA 
GCAGCAGCCC TATCCGATGA CGAATTATGT CTGTCGGTGA CTGAAAATTT CAAAAAATAC 
GGTGATTTGG CTAGAGTTAA AGTTTTGCGT GATAACGCAA ATAGACCTTA TGCTTTTGTT 
CAATACAACA ACGATCATGA TGCTAAACAT GCTCTGATCC GCGCTCAGGG TACTTTATTA 
AATGGCAGAA GGTTGCGTTG TGAACCTGCA AAAGTGAACA GGACATTGTA CTTGAAAAAC 
CAGCAAAGTA TTGATTTCAA CGAGATCAGT CAAATTTGTG AAAAATTTGG TGGTTTGGAA 
CAGATTGTTC CTGATAGAAC TGATAATCAA TACACGAGAA GATATACTTA TCCGATATCT 
TCGGCAAATT CTTGGTTTGT TCAATTCGTT TACAGAGACG ATGCAATCAG AGCTTATGCG 
AATTTACGAA CTGATCCCAA TTGGATCATC GAATGGGCAC AAAATATAAA CGTCCCAAAA 
AATTATAACC TTTTACACAA AAGCAAATTC AAAAGTTCCA AATACCATCA AAACAACGGT 
ATTATCAATA ACGATGGTAG TAACAACAAC GATAATAATA ATAGTAATAA TAATAATAGA 
GAGGATTCAA GGAGGAATGG CGACGTTATA GAAGAGGAGT GCGGACATGT GCATGGTAGT 
GATTCAGAGG AAAAATTGAC AAGCGATGGT ATTTATGACG ACGAAGATAA AGATTCCGAG 
ATCACCATTG ATAAAAGATC CATTTTCGTG GGTCAATTAG ACAAAGAAAC CACCAGAGAG 
GAATTGAATA GAAGATTTTC CACTCATGGT AAAATCCAAG ATATTAATTT AATCTTCAAG 
CCAACAAACA TATTTGCGTT TATTAAATAT GAAACCGAAG AAGCTGCTGC TGCTGCTTTG 
GAAAGTGAAA ACCATGCAAT TTTTTTGAAT AAGACAATGC ATGTTCAATA TAAAGAGGTA 
GGTGGTCGTC ACAACAGGAA GTTCTCTGGT AAGAACGGTG GCAGCAACTT TAACCATCAT 
CAGTTTTTTA GCACCAGATC GGGCAAAACA TTTACAGGGC CAGAACTGAA CTTAGCACCA 
CCACCAATCA ACATGTACAG GAAAATGAGC GGCGGATCCC AGCAGGAAAG CGAAACTATG 
ATGCCATACA TGCCCATGGG GCCCATGCCC ATGGGGCCTC CACCACCAAA TGCAGCAAGT 
CTCAGTGATT TTGATATGTT CCCCCCAAGT TACTCCACGT TTATGAAGGG AATGATGCCA 
TTGAGGCGTA AATCTATGCC CAATTCTTGG TCATCCCCTT CTTCAAAGAG TGTGAACTCT 
GAAAATGAAA GTGTTAATGG TGGTGATGAG AATTCCGAAT TGCCTTCCGA AATTCCTGAA 
TCATCTGGTA GATATAATGC TGCTAATTCC TTTACCACAT ATAATAATTC CTCAGCAGGA 
AACTCTAACA ACAACAACAA CAACAACAAC AGTAACAGTA ATAAGTCTCA ATATAAAAAG 
CGCTATGCAA GGAGGTCAAG CTACGGATAC AATGAAGTAC CTCCAAAACC ATACTATTTC 
CAGCCTTATT ACTATCACCC CATGCAATAC CATATGGGCC CAATGGGCCC ATTGCATCCT 
TCTCAAGGTT CTGCTGGAAA TCACCATCCA TATATGATGG TTTATCCCAT GTCACCACCT 
CCGCCTTCTG GGTTAGATGG AAGCATGATC CCTCCTCCAA TAAATGTAAG CCAATCACAT 
GCTGCTAATC ATGGCAGTAC CCATGTTCAT GCGAACGAAT TCATATCAAA CGATACTGGT 
GACATAAATG AAGATAACAA AGCTTATAGT TTAGATTACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011839.1
CDS	1..2142
Translation	MKTEISTADSLRDPPSNGLKADSELVIREDIDQFLPSEVSSLGSDHQNDGEDSDTDSDNFLQDPEDDVDEESTGRGTVTTTSTSTESRGRPSSCIFVASLAAALSDDELCLSVTENFKKYGDLARVKVLRDNANRPYAFVQYNNDHDAKHALIRAQGTLLNGRRLRCEPAKVNRTLYLKNQQSIDFNEISQICEKFGGLEQIVPDRTDNQYTRRYTYPISSANSWFVQFVYRDDAIRAYANLRTDPNWIIEWAQNINVPKNYNLLHKSKFKSSKYHQNNGIINNDGSNNNDNNNSNNNNREDSRRNGDVIEEECGHVHGSDSEEKLTSDGIYDDEDKDSEITIDKRSIFVGQLDKETTREELNRRFSTHGKIQDINLIFKPTNIFAFIKYETEEAAAAALESENHAIFLNKTMHVQYKEVGGRHNRKFSGKNGGSNFNHHQFFSTRSGKTFTGPELNLAPPPINMYRKMSGGSQQESETMMPYMPMGPMPMGPPPPNAASLSDFDMFPPSYSTFMKGMMPLRRKSMPNSWSSPSSKSVNSENESVNGGDENSELPSEIPESSGRYNAANSFTTYNNSSAGNSNNNNNNNNSNSNKSQYKKRYARRSSYGYNEVPPKPYYFQPYYYHPMQYHMGPMGPLHPSQGSAGNHHPYMMVYPMSPPPPSGLDGSMIPPPINVSQSHAANHGSTHVHANEFISNDTGDINEDNKAYSLDY

Target ORF information:

RefSeq Version	NM_001179104.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Rim4p (RIM4), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179104.1

ORF Insert Sequence:

ATGAAAACCG AAATCAGCAC TGCGGATTCC CTTCGTGATC CTCCCTCTAA TGGTCTAAAG 
GCCGACTCTG AGCTGGTTAT CAGAGAGGAT ATAGACCAAT TTTTGCCTTC GGAAGTATCT 
TCGTTGGGGT CAGATCACCA GAATGATGGT GAGGATTCAG ACACTGACAG TGACAACTTT 
TTGCAAGACC CTGAAGACGA TGTGGATGAA GAAAGCACTG GTAGAGGTAC AGTCACTACC 
ACTTCCACAT CCACTGAGTC AAGAGGCCGT CCATCTTCTT GTATCTTCGT GGCAAGCTTA 
GCAGCAGCCC TATCCGATGA CGAATTATGT CTGTCGGTGA CTGAAAATTT CAAAAAATAC 
GGTGATTTGG CTAGAGTTAA AGTTTTGCGT GATAACGCAA ATAGACCTTA TGCTTTTGTT 
CAATACAACA ACGATCATGA TGCTAAACAT GCTCTGATCC GCGCTCAGGG TACTTTATTA 
AATGGCAGAA GGTTGCGTTG TGAACCTGCA AAAGTGAACA GGACATTGTA CTTGAAAAAC 
CAGCAAAGTA TTGATTTCAA CGAGATCAGT CAAATTTGTG AAAAATTTGG TGGTTTGGAA 
CAGATTGTTC CTGATAGAAC TGATAATCAA TACACGAGAA GATATACTTA TCCGATATCT 
TCGGCAAATT CTTGGTTTGT TCAATTCGTT TACAGAGACG ATGCAATCAG AGCTTATGCG 
AATTTACGAA CTGATCCCAA TTGGATCATC GAATGGGCAC AAAATATAAA CGTCCCAAAA 
AATTATAACC TTTTACACAA AAGCAAATTC AAAAGTTCCA AATACCATCA AAACAACGGT 
ATTATCAATA ACGATGGTAG TAACAACAAC GATAATAATA ATAGTAATAA TAATAATAGA 
GAGGATTCAA GGAGGAATGG CGACGTTATA GAAGAGGAGT GCGGACATGT GCATGGTAGT 
GATTCAGAGG AAAAATTGAC AAGCGATGGT ATTTATGACG ACGAAGATAA AGATTCCGAG 
ATCACCATTG ATAAAAGATC CATTTTCGTG GGTCAATTAG ACAAAGAAAC CACCAGAGAG 
GAATTGAATA GAAGATTTTC CACTCATGGT AAAATCCAAG ATATTAATTT AATCTTCAAG 
CCAACAAACA TATTTGCGTT TATTAAATAT GAAACCGAAG AAGCTGCTGC TGCTGCTTTG 
GAAAGTGAAA ACCATGCAAT TTTTTTGAAT AAGACAATGC ATGTTCAATA TAAAGAGGTA 
GGTGGTCGTC ACAACAGGAA GTTCTCTGGT AAGAACGGTG GCAGCAACTT TAACCATCAT 
CAGTTTTTTA GCACCAGATC GGGCAAAACA TTTACAGGGC CAGAACTGAA CTTAGCACCA 
CCACCAATCA ACATGTACAG GAAAATGAGC GGCGGATCCC AGCAGGAAAG CGAAACTATG 
ATGCCATACA TGCCCATGGG GCCCATGCCC ATGGGGCCTC CACCACCAAA TGCAGCAAGT 
CTCAGTGATT TTGATATGTT CCCCCCAAGT TACTCCACGT TTATGAAGGG AATGATGCCA 
TTGAGGCGTA AATCTATGCC CAATTCTTGG TCATCCCCTT CTTCAAAGAG TGTGAACTCT 
GAAAATGAAA GTGTTAATGG TGGTGATGAG AATTCCGAAT TGCCTTCCGA AATTCCTGAA 
TCATCTGGTA GATATAATGC TGCTAATTCC TTTACCACAT ATAATAATTC CTCAGCAGGA 
AACTCTAACA ACAACAACAA CAACAACAAC AGTAACAGTA ATAAGTCTCA ATATAAAAAG 
CGCTATGCAA GGAGGTCAAG CTACGGATAC AATGAAGTAC CTCCAAAACC ATACTATTTC 
CAGCCTTATT ACTATCACCC CATGCAATAC CATATGGGCC CAATGGGCCC ATTGCATCCT 
TCTCAAGGTT CTGCTGGAAA TCACCATCCA TATATGATGG TTTATCCCAT GTCACCACCT 
CCGCCTTCTG GGTTAGATGG AAGCATGATC CCTCCTCCAA TAAATGTAAG CCAATCACAT 
GCTGCTAATC ATGGCAGTAC CCATGTTCAT GCGAACGAAT TCATATCAAA CGATACTGGT 
GACATAAATG AAGATAACAA AGCTTATAGT TTAGATTACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)
Johnston M,Andrews S,Brinkman R,Cooper J,Ding H,Dover J,Du Z,Favello A,Fulton L,Gattung S

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RIM4 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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