RIM101 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	RIM101
Entrez Gene ID	856358
Full Name	alkaline-responsive transcriptional regulator RIM101
Synonyms	RIM1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179107.1	NP_011836.1	alkaline-responsive transcriptional regulator RIM101

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0E00793g	XP_453982.1
Eremothecium gossypii	AGOS_AFR190C	NP_985737.1
Saccharomyces cerevisiae (baker's yeast)	RIM101	NP_011836.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Johnston M, Andrews S, Brinkman R, Cooper J, Ding H, Dover J, Du Z, Favello A, Fulton L, Gattung S
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results suggest that the Rim101 pathway is important for the adaptation to ER stress by compensating for alterations in plasma membrane lipid asymmetry induced by ER stress.
Title: The Rim101 pathway contributes to ER stress adaptation through sensing the state of plasma membrane.

We conclude that summation operator1278b may harbor a variant(s) that buffers against widespread transcriptional dysregulation upon introduction of a non-native RIM101 allele, emphasizing the importance of accounting for genetic background .
Title: A trans-acting Variant within the Transcription Factor RIM101 Interacts with Genetic Background to Determine its Regulatory Capacity.

These results show that, in response to salt stress, a functional Rim complex via specific ESCRT interactions is required for the proper accumulation of the Ena1 protein, but not induction of the ENA1 gene.
Title: A functional Rim101 complex is required for proper accumulation of the Ena1 Na+-ATPase protein in response to salt stress in Saccharomyces cerevisiae.

A role of Rim101 in modulation of vacuolar acidity necessary for selenite detoxification is discovered.
Title: The PacC-family protein Rim101 prevents selenite toxicity in Saccharomyces cerevisiae by controlling vacuolar acidification.

Results found that activity of the Rsp5 ubiquitin ligase was required for recruiting the downstream molecules to the plasma membrane, suggesting that ubiquitination mediates Rim101 signaling at the plasma membrane.
Title: Signaling events of the Rim101 pathway occur at the plasma membrane in a ubiquitination-dependent manner.

The following RIM101 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RIM101 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi05168	NM_001179107.1 Latest version!	Saccharomyces cerevisiae S288C alkaline-responsive transcriptional regulator RIM101 (RIM101), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RIM101 ( NM_001179107.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011836.1 Saccharomyces cerevisiae S288C alkaline-responsive transcriptional regulator RIM101 (RIM101), partial mRNA.

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CloneID	OSi05168
Clone ID Related Accession (Same CDS sequence)	NM_001179107.1
Accession Version	NM_001179107.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	alkaline-responsive transcriptional regulator RIM101
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001140). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Veresion :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGTGCCAT TGGAAGATCT GCTTAATAAA GAAAATGGCA CTGCCGCACC TCAACACAGC 
CGGGAGAGTA TAGTAGAAAA TGGGACAGAC GTTTCTAACG TCACAAAGAA GGACGGGCTG 
CCCAGTCCTA ACCTATCTAA AAGGTCTTCT GATTGTTCGA AGAGGCCTAG AATTAGGTGT 
ACCACGGAAG CTATTGGCTT GAACGGTCAA GAAGATGAAC GGATGTCACC AGGCAGCACT 
TCTTCATCAT GCTTACCATA TCATAGTACT TCACACTTGA ACACTCCTCC ATACGATCTA 
TTGGGCGCTT CGGCAGTTTC ACCCACCACA TCATCTTCCT CTGACTCGTC CTCCTCCTCG 
CCATTGGCAC AAGCGCATAA CCCGGCAGGT GATGACGACG ATGCGGATAA TGACGGTGAT 
TCTGAGGATA TAACACTATA TTGTAAATGG GACAACTGTG GTATGATTTT CAACCAACCA 
GAGCTGCTAT ATAATCATCT GTGTCATGAC CATGTTGGCA GGAAGTCACA TAAGAACCTA 
CAATTGAATT GTCATTGGGG AGACTGTACC ACGAAGACGG AGAAAAGGGA CCATATCACC 
TCGCATTTGA GAGTTCACGT ACCATTGAAA CCGTTCGGTT GCTCTACGTG CTCCAAAAAG 
TTTAAACGTC CGCAAGATTT AAAAAAGCAT TTGAAAATTC ATCTGGAAAG TGGCGGTATT 
TTGAAAAGAA AGAGGGGACC CAAATGGGGC TCAAAAAGAA CTTCTAAGAA AAACAAAAGT 
TGTGCAAGCG ACGCAGTGTC GTCTTGTTCT GCATCTGTGC CTTCCGCTAT AGCTGGTAGC 
TTTAAGTCAC ATTCTACTTC TCCACAGATA TTACCCCCAT TGCCCGTGGG TATATCTCAA 
CATCTGCCTT CACAACAGCA GCAGCGGGCT ATTTCCTTAA ACCAACTATG CTCAGACGAG 
CTATCTCAGT ATAAGCCGGT ATATTCTCCA CAATTGAGTG CCAGATTGCA AACGATCTTG 
CCGCCATTAT ACTACAATAA CGGCAGCACG GTAAGCCAAG GTGCCAACAG TCGGAGCATG 
AATGTCTACG AGGACGGTTG TTCTAATAAG ACCATAGCAA ACGCCACCCA ATTCTTCACC 
AAACTATCGA GAAATATGAC AAATAACTAC ATTTTGCAAC AAAGCGGTGG TAGTACTGAG 
TCCTCGTCAT CATCTGGGCG CATCCCCGTA GCACAAACAA GCTATGTGCA GCCACCTAAT 
GCTCCTTCAT ACCAATCCGT ACAAGGTGGT AGCTCTATTT CGGCCACTGC AAATACAGCA 
ACTTATGTGC CAGTACGGTT GGCCAAGTAT CCAACTGGTC CTTCGCTTAC TGAACATTTG 
CCACCTTTAC ATTCGAACAC TGCCGGTGGT GTTTTCAACC GCCAATCACA GTATGCTATG 
CCACACTATC CATCTGTTCG TGCTGCGCCT AGCTATTCCT CCAGTGGCTG TTCTATATTA 
CCGCCTCTAC AATCAAAGAT ACCAATGTTA CCATCGCGCC GTACAATGGC AGGTGGAACT 
TCATTGAAGC CTAACTGGGA ATTTAGCCTG AACCAAAAAA GCTGTACTAA TGATATTATC 
ATGAGTAAGC TGGCGATTGA AGAAGTAGAT GACGAAAGCG AGATAGAGGA TGATTTCGTA 
GAGATGTTGG GTATAGTGAA CATTATCAAG GACTACTTGT TGTGCTGTGT CATGGAAGAT 
TTAGATGATG AAGAAAGTGA GGACAAAGAT GAAGAAAACG CATTCTTGCA AGAATCTTTA 
GAAAAATTGA GTTTACAAAA CCAAATGGGA ACTAACTCAG TTCGCATCTT GACCAAGTAT 
CCCAAAATTT TGGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011836.1
CDS	1..1878
Translation	MVPLEDLLNKENGTAAPQHSRESIVENGTDVSNVTKKDGLPSPNLSKRSSDCSKRPRIRCTTEAIGLNGQEDERMSPGSTSSSCLPYHSTSHLNTPPYDLLGASAVSPTTSSSSDSSSSSPLAQAHNPAGDDDDADNDGDSEDITLYCKWDNCGMIFNQPELLYNHLCHDHVGRKSHKNLQLNCHWGDCTTKTEKRDHITSHLRVHVPLKPFGCSTCSKKFKRPQDLKKHLKIHLESGGILKRKRGPKWGSKRTSKKNKSCASDAVSSCSASVPSAIAGSFKSHSTSPQILPPLPVGISQHLPSQQQQRAISLNQLCSDELSQYKPVYSPQLSARLQTILPPLYYNNGSTVSQGANSRSMNVYEDGCSNKTIANATQFFTKLSRNMTNNYILQQSGGSTESSSSSGRIPVAQTSYVQPPNAPSYQSVQGGSSISATANTATYVPVRLAKYPTGPSLTEHLPPLHSNTAGGVFNRQSQYAMPHYPSVRAAPSYSSSGCSILPPLQSKIPMLPSRRTMAGGTSLKPNWEFSLNQKSCTNDIIMSKLAIEEVDDESEIEDDFVEMLGIVNIIKDYLLCCVMEDLDDEESEDKDEENAFLQESLEKLSLQNQMGTNSVRILTKYPKILV

Target ORF information:

RefSeq Version	NM_001179107.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C alkaline-responsive transcriptional regulator RIM101 (RIM101), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179107.1

ORF Insert Sequence:

ATGGTGCCAT TGGAAGATCT GCTTAATAAA GAAAATGGCA CTGCCGCACC TCAACACAGC 
CGGGAGAGTA TAGTAGAAAA TGGGACAGAC GTTTCTAACG TCACAAAGAA GGACGGGCTG 
CCCAGTCCTA ACCTATCTAA AAGGTCTTCT GATTGTTCGA AGAGGCCTAG AATTAGGTGT 
ACCACGGAAG CTATTGGCTT GAACGGTCAA GAAGATGAAC GGATGTCACC AGGCAGCACT 
TCTTCATCAT GCTTACCATA TCATAGTACT TCACACTTGA ACACTCCTCC ATACGATCTA 
TTGGGCGCTT CGGCAGTTTC ACCCACCACA TCATCTTCCT CTGACTCGTC CTCCTCCTCG 
CCATTGGCAC AAGCGCATAA CCCGGCAGGT GATGACGACG ATGCGGATAA TGACGGTGAT 
TCTGAGGATA TAACACTATA TTGTAAATGG GACAACTGTG GTATGATTTT CAACCAACCA 
GAGCTGCTAT ATAATCATCT GTGTCATGAC CATGTTGGCA GGAAGTCACA TAAGAACCTA 
CAATTGAATT GTCATTGGGG AGACTGTACC ACGAAGACGG AGAAAAGGGA CCATATCACC 
TCGCATTTGA GAGTTCACGT ACCATTGAAA CCGTTCGGTT GCTCTACGTG CTCCAAAAAG 
TTTAAACGTC CGCAAGATTT AAAAAAGCAT TTGAAAATTC ATCTGGAAAG TGGCGGTATT 
TTGAAAAGAA AGAGGGGACC CAAATGGGGC TCAAAAAGAA CTTCTAAGAA AAACAAAAGT 
TGTGCAAGCG ACGCAGTGTC GTCTTGTTCT GCATCTGTGC CTTCCGCTAT AGCTGGTAGC 
TTTAAGTCAC ATTCTACTTC TCCACAGATA TTACCCCCAT TGCCCGTGGG TATATCTCAA 
CATCTGCCTT CACAACAGCA GCAGCGGGCT ATTTCCTTAA ACCAACTATG CTCAGACGAG 
CTATCTCAGT ATAAGCCGGT ATATTCTCCA CAATTGAGTG CCAGATTGCA AACGATCTTG 
CCGCCATTAT ACTACAATAA CGGCAGCACG GTAAGCCAAG GTGCCAACAG TCGGAGCATG 
AATGTCTACG AGGACGGTTG TTCTAATAAG ACCATAGCAA ACGCCACCCA ATTCTTCACC 
AAACTATCGA GAAATATGAC AAATAACTAC ATTTTGCAAC AAAGCGGTGG TAGTACTGAG 
TCCTCGTCAT CATCTGGGCG CATCCCCGTA GCACAAACAA GCTATGTGCA GCCACCTAAT 
GCTCCTTCAT ACCAATCCGT ACAAGGTGGT AGCTCTATTT CGGCCACTGC AAATACAGCA 
ACTTATGTGC CAGTACGGTT GGCCAAGTAT CCAACTGGTC CTTCGCTTAC TGAACATTTG 
CCACCTTTAC ATTCGAACAC TGCCGGTGGT GTTTTCAACC GCCAATCACA GTATGCTATG 
CCACACTATC CATCTGTTCG TGCTGCGCCT AGCTATTCCT CCAGTGGCTG TTCTATATTA 
CCGCCTCTAC AATCAAAGAT ACCAATGTTA CCATCGCGCC GTACAATGGC AGGTGGAACT 
TCATTGAAGC CTAACTGGGA ATTTAGCCTG AACCAAAAAA GCTGTACTAA TGATATTATC 
ATGAGTAAGC TGGCGATTGA AGAAGTAGAT GACGAAAGCG AGATAGAGGA TGATTTCGTA 
GAGATGTTGG GTATAGTGAA CATTATCAAG GACTACTTGT TGTGCTGTGT CATGGAAGAT 
TTAGATGATG AAGAAAGTGA GGACAAAGAT GAAGAAAACG CATTCTTGCA AGAATCTTTA 
GAAAAATTGA GTTTACAAAA CCAAATGGGA ACTAACTCAG TTCGCATCTT GACCAAGTAT 
CCCAAAATTT TGGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)
Johnston M,Andrews S,Brinkman R,Cooper J,Ding H,Dover J,Du Z,Favello A,Fulton L,Gattung S

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RIM101 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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