ECM29 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	ECM29
Entrez Gene ID	856355
Full Name	Ecm29p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179110.1	NP_011833.1	Ecm29p

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	ECM29	NP_011833.1
Magnaporthe oryzae (rice blast fungus)	MGG_08744	XP_003719241.1
Eremothecium gossypii	AGOS_ACL063W	NP_983341.2
Kluyveromyces lactis	KLLA0E00727g	XP_453979.1
Neurospora crassa	NCU00770	XP_964926.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Johnston M, Andrews S, Brinkman R, Cooper J, Ding H, Dover J, Du Z, Favello A, Fulton L, Gattung S
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that Ecm29 acts as a proteasome inhibitor.
Title: The proteasome-associated protein Ecm29 inhibits proteasomal ATPase activity and in vivo protein degradation by the proteasome.

Loss of Rpt5 protein interactions with the core particle and Nas2 protein causes the formation of faulty proteasomes that are inhibited by Ecm29 protein.
Title: Loss of Rpt5 protein interactions with the core particle and Nas2 protein causes the formation of faulty proteasomes that are inhibited by Ecm29 protein.

results characterize Ecm29 as a proteasome chaperone whose appropriate interaction with the proteasome requires Not4
Title: Not4 E3 ligase contributes to proteasome assembly and functional integrity in part through Ecm29.

Studies indicate that deletion of ECM29 prevented the disassembly of the 26S proteasome in response to oxidative stress, and ecm29 mutants were more sensitive to HO than were wild-type cells.
Title: Regulation of the 26S proteasome complex during oxidative stress.

The following ECM29 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ECM29 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi05165	NM_001179110.1 Latest version!	Saccharomyces cerevisiae S288C Ecm29p (ECM29), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ECM29 ( NM_001179110.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011833.1 Saccharomyces cerevisiae S288C Ecm29p (ECM29), partial mRNA.

Price & Availability↑

CloneID	OSi05165
Clone ID Related Accession (Same CDS sequence)	NM_001179110.1
Accession Version	NM_001179110.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5607bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Ecm29p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001140). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Veresion :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCCATTT CTTCTGACGA AGCGAAAGAA AAACAGCTCG TGGAGAAGGC AGAATTGCGC 
CTGGCTATCG CAGATTCCCC TCAAAAGTTC GAAACTAATC TGCAGACTTT TTTGCCTCCA 
TTATTACTAA AACTCGCCTC TCCACATGCC TCCGTTAGAA CAGCAGTATT TTCAGCCTTA 
AAAAACCTCA TTTCTAGGAT AAATACGCTG CCCCAGGTTC AGCTTCCTGT GAGGGCGCTT 
ATTGTCCAAG CAAAGGAACC CAATTTAGCC GCTCAACAAG ACTCGACCAA TGTCCGCCTT 
TACAGTCTGC TACTGGCGTC TAAAGGCATA GATAGGCTTT CCTTGCAAGA TAGACAGCAG 
TTGCTACCGT TAGTCGTTTC GAACATCTCG TGTCTAACAG GCACAGTAGC CGCCAGAATG 
TTCCACATCC TTTTGAAGCT TATTTTGGAA TGGGTTGCAC CCCAGGAGTC TTCACATGAG 
CAGGAAGAAT TTGTTCAGTT CCTTCAACTG GACAACGACG GTTTTTCGTT TTTGATGCGC 
CAATTTACCA GGTTTTTTCT GCTGGTACCT TCAAAGCAAG TCCAGGTTTC TCAACAGCCC 
CTGTCGAGAG GTTACACATG TCCGGGCCTA TCTCTAACTG ATGTGGCATT CTTTACTTAT 
GATGCGGGCG TTACTTTCAA CAAAGAACAG CTGAATAAGT TCAAAAAGGC TATTTTTCAA 
TTTGTTTGCC GTGGCATGGC GGCTACCCAA ACTATTGAGC AGTCACCTAG GATGATTGAA 
CTGATGGAAT TTTTGTGCGT TGTCTCTACG GATTCTACAA ATTTATCTGA TGATGCGGCT 
CAGTTCATGA AACGGTTTCC CATGCCTTAT GAAAACGAAG AGTTCATCAC CTTTCTTCAG 
ACGCTGTACA TCGGCAACAC AGCAAATGGA AGACCGCCGG TCAAGGCTAT CTTGCAAGAA 
AAAATCCTGT CCATATTAAA CAGAAGTCAC TTCGCCACCA CCAAAGCAGA ATGTATTTCG 
TTGATTTGCT CTATTGGATT GCATTCATCT GAGTATAAAC TTAGATCTTT GACTCTTTCT 
TTTATTCGAC ACGTAGCAAA ATTAAATTAC AAAAATTTAA ATCCTGCTTC TTCATCACCT 
TCATCAACAG ACTTTTCCAC TTGTATTGTT TCCTTGATTA GAAATAATCT TCATGCCGAA 
GGTTGGCCGA AGTTACAATT GGGGCCTCAG ACCCCAGCTT TCAATACAGC CATTCTGCAG 
AGACAGCTGC AATATGAAAC ATTGGGTGAT ATCTTGAAAA GAGATTTTGA GCTTGTAAGT 
GACTTATCCT ATATTGAATT TCTATTCGAG TCCTTGAAAA ATGACTTACC ACAGTTCCGC 
TCCAGTATTC AGGAGTCTCT GCTTTCTCTA GTAGGCCATT TATCAATTCT ACCCCAACAA 
TCTAAATTAA AATTAAAAAA TTTGCTCAGG AAAAACCTGT CAATTGACGA GCAACAGCGG 
GAGGATAATA ATGATGCTGT TAATTCTATA ATGGCACTCA AATTTGTATC TATAAAATTC 
ACTAACGCTG CCTTCCCCTT CCATGATCCT GAGGCAAGGT TATTCAATAT TTGGGGTACT 
GTACGAACGA ATAGATTTGA CATTATTGAA GAATCCTTTA AGGGGCTTCA ACCCTTTTGG 
TTCAGGGTTA ATAATGCTTC CATAAATACA TCAGCTACCG TGAAAACATC CGATCTGTTG 
GGCTCTCACT TGTCAGAGAC TGAATTTCCT CCATTCCGGG AATTTCTTCA GGTTTTGATC 
GACCAGCTAG ATTCGGAAGC TGCATCAATT ACACGAAAAT CACTGAACAA CGCTGTCAGA 
TTTAGTAAAC AATGTCTCAT TTCCAATGCA ATATACGGTA AAAAAACAAT GGTTATACAA 
GATGAGGATT GGTCAGTTAG AATAGACAAA GCTTTGGAAC TTGACGACAC AGTTGTGTCC 
CGAGTTAATG AAATGGTTCA AGGCATGAAT GATGATATTT TCATTCGCTA TTTGACTCTT 
CTGTCAAACG AGTTTACAGC TACGAACAGT AAGGGTGAAC AAATTGCAAT ATTTCCATAT 
CAAGATCCAA TTTTTGGTTC TGTCTTATTA ACTTTATTGA ATTTTGTGAG CAATAATGTT 
TTACGGAGAT TGGAAATTCT TGTTCCCGAT TTATACCATC TAGTTATCAT GAAATTTCAA 
TCGTTGAGTG ATAATGATCT TGCAGTCTGT GCAACCATCA TCGGTATAAT TTCTACTGCT 
ATCGCAGACT CGACTCACGT TAAGCGAATC ACAAAAATAG CACAATCACA AACAATGGCA 
GAGACATATG TTGCATCTTA TGTTGTTCCA CGATTATATT TGAAAGATCA AACAAATCAC 
ATTGAATCTG ACAGTATCCT AAACCTATTA AACATTTTAA CAACTCATCT ATCGCATCCT 
GGCACTAACA AGGATATGAT ATTAAAGTTG GTCTGCCAAG TGACAAAATT CGGTTTACTT 
CTCCAAGTAA GTGCACAAGA AAGGAAAGAT TTCTTGAAAA AAGTCATGGA TACTATACAA 
GACAAATTGA TCAACGATGT GACAGCAATT CAAACGTGGT CATACCTATC ACTATATTCA 
ACAGATTTAG AGAATTCCAG TCTTTTTCAA GAAAAATTAC TTGAAACTAA CGTTTCTAAG 
CAAAACGATT TCTTATTTTC AGTTGGTGAA TCTTTAAGTG TTGTGGCTGG TAAATGGTCA 
AGTAAGTACT TGATCAAACA GATTGATATT CCTAATTTCA ATGTTGAGAT CATGCAACAA 
AAATTCCCTG CTACAAATGT TACCACTATA CTTGATGAGA TCTTTTCAGG TTGCGACTCT 
ACAAAACCCT CTCTGAGGAA GGCATCTTGT ATTTGGCTGT TATCATATAT TCAGTACTTA 
GGTCATTTGC CAGAAGTAAG TTCCAAATGT AATGATATTC ACTTGAGATT TATGAGATTT 
TTGGCAGACA GAGATGAATT TATACAGGAT TCCGCCGCTA GAGGGCTTTC TTTGGTTTAC 
GAAATTGGTG GTTCTGATTT AAAAGAAAGT ATGGTCAAAG GGCTACTTAA ATCATTTACA 
GAATCAACCG CAGGGTCTGC ATCTACAAGT GCTACTGGCA TCTCAGGTTC AGTCTCCGAA 
GAGACCGAGT TATTTGAGCC AGGAGTTTTA AACACTGGAG ATGGGTCCAT TAGCACTTAC 
AAAGATATTT TGAATTTGGC ATCTGAAGTT GGGGACCCCG CACTTGTTTA CAAATTTATG 
TCTCTTGCCA AAAGTTCTGC GCTATGGTCA TCTAGGAAAG GTATCGCATT CGGTCTTGGC 
GCCATTATGT CTAAATCTTC TTTAGAAGAA TTATTACTAA AGGATCAGCA AACTGCCAAG 
AAATTAATTC CAAAATTGTA TAGATACAGA TTTGATCCAT TTCAAGCAGT ATCACGCTCG 
ATGACAGACA TCTGGAATAC TTTAATTCCA GAATCCTCTT TGACGATTTC CCTCTATTTC 
AATGATATTC TTGATGAGCT TTTATGTGGC ATGGCTAATA AAGAATGGAG GGTCAGGGAG 
GCCAGTACTT CAGCTTTATT ACAATTGATT CAATCTCAAC CGCAAGAGAA ATTTTCCGAA 
AAAATGCTGA AAATTTGGAC AATGGCATTC AGAACGATGG ATGATATAAA AGATAGTGTC 
CGTGAAGTTG GTACCAAGTT CACCACTGTA TTGGCCAAAA TTTTGGCAAG ATCTATTGAT 
GTGGAAAAAG GTGTGAACCC TACCAAGTCT AAGGAAATAC TAGACAATAT TTTGCCATTT 
TTATGGGGTC CACATGGTCT GAACAGTGAT GCAGAAGAGG TTAGAAATTT TGCGCTCACA 
ACTTTGATTG ACTTGGTGAA ACACTCCCCT GGAGCCATCA AACCGTTCAC TCCAAAATTA 
ATTTACGATT TTATCACATT GTTTTCTTCC ATTGAACCTC AGGTGATTAA CTATTTGGCA 
TTAAATGCTG CAAATTATAA CATTGATGCT AACGTCATTG ATACGCAAAG GAAAAATGGT 
GTCACAAATT CACCTCTATT CCAGACCATC GAAAAATTAA TTAACAATTC AGATGATTGT 
ATGATGGAAG AAATAATTAA TGTGGTTATT AAAGCTAGCA GAAAATCAGT CGGATTGCCA 
TCTAAGGTCG CATCCTCACT TGTCATAATT ATCTTAGTGA AACGGTACTC TATCGAAATG 
AAACCCTATT CTGGAAAGTT GTTGAAAGTC TGCTTGACTA TGTTTGAAGA TAGAAATGAG 
TCGGTTAATA TAGCATTTGC AATTTCAATG GGATATTTAT TCAAGGTTTC AGCGTTGGAT 
AAGTGTATTA AATACTCTGA AAAACTAATA ACGAAATATT TTGAGCCTAC CTCAACAGAA 
AATAACAAGA AAGTTGTTGG TACAGCGATC GACTCGATTT TGAATTATGC GAAGTCAGAA 
TTCGATAATG TGGCAAGTGT ATTTATGCCT TTGATTTTCA TAGCATGCAA TGATGAAGAT 
AAAGATTTGG AAACCTTATA TAATAAAATC TGGACGGAAG CTTCCAGCTC TGGCGCCGGT 
ACGGTTAAAT TATACCTGCC AGAAATTTTA AACGTACTTT GTGTGAATAT AAAGTCAAAT 
GATTTCTCTA TTCGAAAAAC ATGTGCTAAA TCAGTCATAC AGTTGTGTGG CGGTATTAAT 
GATAGTATTC CTTATCCACA AATTGTTAAG TTATTTGATA TATCGAGGGA GGCATTAAGT 
GGTAGATCAT GGGATGGTAA AGAGCATATA GTGGCAGCAT TGGTTTCATT AACAGAAAAA 
TTTTCTCAAA CTGTGGCCGA CAATAATGAT CTACAAGAAT CAATTAATCA TGTAATGTAC 
ACTGAAGTTT CGAGAAAAAG TATGAAATAT GTTAAGAAGA TACTACCGCT TTACGCGAGA 
TATATCAATG TGAATCCACA AGAAGAGACG ATCACCTTCT TAATTGAAAA AGCCAAAGAA 
ATGATTCGAT TGTTGGGCAG CGAATCTGAT GATAGTGAAG GTTCCATTAA GCAGACTTCC 
GATGAATCCA CAATAAAAAG AATTAAGCCT AACACTGAAA TTACACAAAA GTCATCCAAA 
GAGAACATTG AGAACGAAGA GTATGTGATC AACTTGTTAA AAGTTAGTGT TGACATATGC 
AACAATTCTA AATCGAGGTA TCCGATGAAT TTATTGGAGT TCATTATAGA TGAGATTGCA 
TACCTTTTCC ATAATGACAG AATTATACAC ACATGGAGAA CGCAACTAGC TGCTAGTGAA 
ATTGGTATCT CGATAGTTGG AAGATTTAGT ACCATAAGTA GCGCTGACTT TATCCAAAAT 
GTGGGGAGAC TATGGGATCA AACTTTTCCA ATAAATTGTA ACAAGGAAAC CATTGAGAAT 
GTGAAATTGC AGATGATAAA GTTCGGTGGC CTGATCATTC AGAAGATTCC AAGCTTGCAG 
AACAATATCG AGGAAAACTT ACGGTTGTTG AACAGTATTG ATTCAACGAG CAGAATTGAG 
TTGGAATTGA AAAATATTGG TCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011833.1
CDS	1..5607
Translation	MSISSDEAKEKQLVEKAELRLAIADSPQKFETNLQTFLPPLLLKLASPHASVRTAVFSALKNLISRINTLPQVQLPVRALIVQAKEPNLAAQQDSTNVRLYSLLLASKGIDRLSLQDRQQLLPLVVSNISCLTGTVAARMFHILLKLILEWVAPQESSHEQEEFVQFLQLDNDGFSFLMRQFTRFFLLVPSKQVQVSQQPLSRGYTCPGLSLTDVAFFTYDAGVTFNKEQLNKFKKAIFQFVCRGMAATQTIEQSPRMIELMEFLCVVSTDSTNLSDDAAQFMKRFPMPYENEEFITFLQTLYIGNTANGRPPVKAILQEKILSILNRSHFATTKAECISLICSIGLHSSEYKLRSLTLSFIRHVAKLNYKNLNPASSSPSSTDFSTCIVSLIRNNLHAEGWPKLQLGPQTPAFNTAILQRQLQYETLGDILKRDFELVSDLSYIEFLFESLKNDLPQFRSSIQESLLSLVGHLSILPQQSKLKLKNLLRKNLSIDEQQREDNNDAVNSIMALKFVSIKFTNAAFPFHDPEARLFNIWGTVRTNRFDIIEESFKGLQPFWFRVNNASINTSATVKTSDLLGSHLSETEFPPFREFLQVLIDQLDSEAASITRKSLNNAVRFSKQCLISNAIYGKKTMVIQDEDWSVRIDKALELDDTVVSRVNEMVQGMNDDIFIRYLTLLSNEFTATNSKGEQIAIFPYQDPIFGSVLLTLLNFVSNNVLRRLEILVPDLYHLVIMKFQSLSDNDLAVCATIIGIISTAIADSTHVKRITKIAQSQTMAETYVASYVVPRLYLKDQTNHIESDSILNLLNILTTHLSHPGTNKDMILKLVCQVTKFGLLLQVSAQERKDFLKKVMDTIQDKLINDVTAIQTWSYLSLYSTDLENSSLFQEKLLETNVSKQNDFLFSVGESLSVVAGKWSSKYLIKQIDIPNFNVEIMQQKFPATNVTTILDEIFSGCDSTKPSLRKASCIWLLSYIQYLGHLPEVSSKCNDIHLRFMRFLADRDEFIQDSAARGLSLVYEIGGSDLKESMVKGLLKSFTESTAGSASTSATGISGSVSEETELFEPGVLNTGDGSISTYKDILNLASEVGDPALVYKFMSLAKSSALWSSRKGIAFGLGAIMSKSSLEELLLKDQQTAKKLIPKLYRYRFDPFQAVSRSMTDIWNTLIPESSLTISLYFNDILDELLCGMANKEWRVREASTSALLQLIQSQPQEKFSEKMLKIWTMAFRTMDDIKDSVREVGTKFTTVLAKILARSIDVEKGVNPTKSKEILDNILPFLWGPHGLNSDAEEVRNFALTTLIDLVKHSPGAIKPFTPKLIYDFITLFSSIEPQVINYLALNAANYNIDANVIDTQRKNGVTNSPLFQTIEKLINNSDDCMMEEIINVVIKASRKSVGLPSKVASSLVIIILVKRYSIEMKPYSGKLLKVCLTMFEDRNESVNIAFAISMGYLFKVSALDKCIKYSEKLITKYFEPTSTENNKKVVGTAIDSILNYAKSEFDNVASVFMPLIFIACNDEDKDLETLYNKIWTEASSSGAGTVKLYLPEILNVLCVNIKSNDFSIRKTCAKSVIQLCGGINDSIPYPQIVKLFDISREALSGRSWDGKEHIVAALVSLTEKFSQTVADNNDLQESINHVMYTEVSRKSMKYVKKILPLYARYINVNPQEETITFLIEKAKEMIRLLGSESDDSEGSIKQTSDESTIKRIKPNTEITQKSSKENIENEEYVINLLKVSVDICNNSKSRYPMNLLEFIIDEIAYLFHNDRIIHTWRTQLAASEIGISIVGRFSTISSADFIQNVGRLWDQTFPINCNKETIENVKLQMIKFGGLIIQKIPSLQNNIEENLRLLNSIDSTSRIELELKNIGL

Target ORF information:

RefSeq Version	NM_001179110.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Ecm29p (ECM29), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179110.1

ORF Insert Sequence:

ATGTCCATTT CTTCTGACGA AGCGAAAGAA AAACAGCTCG TGGAGAAGGC AGAATTGCGC 
CTGGCTATCG CAGATTCCCC TCAAAAGTTC GAAACTAATC TGCAGACTTT TTTGCCTCCA 
TTATTACTAA AACTCGCCTC TCCACATGCC TCCGTTAGAA CAGCAGTATT TTCAGCCTTA 
AAAAACCTCA TTTCTAGGAT AAATACGCTG CCCCAGGTTC AGCTTCCTGT GAGGGCGCTT 
ATTGTCCAAG CAAAGGAACC CAATTTAGCC GCTCAACAAG ACTCGACCAA TGTCCGCCTT 
TACAGTCTGC TACTGGCGTC TAAAGGCATA GATAGGCTTT CCTTGCAAGA TAGACAGCAG 
TTGCTACCGT TAGTCGTTTC GAACATCTCG TGTCTAACAG GCACAGTAGC CGCCAGAATG 
TTCCACATCC TTTTGAAGCT TATTTTGGAA TGGGTTGCAC CCCAGGAGTC TTCACATGAG 
CAGGAAGAAT TTGTTCAGTT CCTTCAACTG GACAACGACG GTTTTTCGTT TTTGATGCGC 
CAATTTACCA GGTTTTTTCT GCTGGTACCT TCAAAGCAAG TCCAGGTTTC TCAACAGCCC 
CTGTCGAGAG GTTACACATG TCCGGGCCTA TCTCTAACTG ATGTGGCATT CTTTACTTAT 
GATGCGGGCG TTACTTTCAA CAAAGAACAG CTGAATAAGT TCAAAAAGGC TATTTTTCAA 
TTTGTTTGCC GTGGCATGGC GGCTACCCAA ACTATTGAGC AGTCACCTAG GATGATTGAA 
CTGATGGAAT TTTTGTGCGT TGTCTCTACG GATTCTACAA ATTTATCTGA TGATGCGGCT 
CAGTTCATGA AACGGTTTCC CATGCCTTAT GAAAACGAAG AGTTCATCAC CTTTCTTCAG 
ACGCTGTACA TCGGCAACAC AGCAAATGGA AGACCGCCGG TCAAGGCTAT CTTGCAAGAA 
AAAATCCTGT CCATATTAAA CAGAAGTCAC TTCGCCACCA CCAAAGCAGA ATGTATTTCG 
TTGATTTGCT CTATTGGATT GCATTCATCT GAGTATAAAC TTAGATCTTT GACTCTTTCT 
TTTATTCGAC ACGTAGCAAA ATTAAATTAC AAAAATTTAA ATCCTGCTTC TTCATCACCT 
TCATCAACAG ACTTTTCCAC TTGTATTGTT TCCTTGATTA GAAATAATCT TCATGCCGAA 
GGTTGGCCGA AGTTACAATT GGGGCCTCAG ACCCCAGCTT TCAATACAGC CATTCTGCAG 
AGACAGCTGC AATATGAAAC ATTGGGTGAT ATCTTGAAAA GAGATTTTGA GCTTGTAAGT 
GACTTATCCT ATATTGAATT TCTATTCGAG TCCTTGAAAA ATGACTTACC ACAGTTCCGC 
TCCAGTATTC AGGAGTCTCT GCTTTCTCTA GTAGGCCATT TATCAATTCT ACCCCAACAA 
TCTAAATTAA AATTAAAAAA TTTGCTCAGG AAAAACCTGT CAATTGACGA GCAACAGCGG 
GAGGATAATA ATGATGCTGT TAATTCTATA ATGGCACTCA AATTTGTATC TATAAAATTC 
ACTAACGCTG CCTTCCCCTT CCATGATCCT GAGGCAAGGT TATTCAATAT TTGGGGTACT 
GTACGAACGA ATAGATTTGA CATTATTGAA GAATCCTTTA AGGGGCTTCA ACCCTTTTGG 
TTCAGGGTTA ATAATGCTTC CATAAATACA TCAGCTACCG TGAAAACATC CGATCTGTTG 
GGCTCTCACT TGTCAGAGAC TGAATTTCCT CCATTCCGGG AATTTCTTCA GGTTTTGATC 
GACCAGCTAG ATTCGGAAGC TGCATCAATT ACACGAAAAT CACTGAACAA CGCTGTCAGA 
TTTAGTAAAC AATGTCTCAT TTCCAATGCA ATATACGGTA AAAAAACAAT GGTTATACAA 
GATGAGGATT GGTCAGTTAG AATAGACAAA GCTTTGGAAC TTGACGACAC AGTTGTGTCC 
CGAGTTAATG AAATGGTTCA AGGCATGAAT GATGATATTT TCATTCGCTA TTTGACTCTT 
CTGTCAAACG AGTTTACAGC TACGAACAGT AAGGGTGAAC AAATTGCAAT ATTTCCATAT 
CAAGATCCAA TTTTTGGTTC TGTCTTATTA ACTTTATTGA ATTTTGTGAG CAATAATGTT 
TTACGGAGAT TGGAAATTCT TGTTCCCGAT TTATACCATC TAGTTATCAT GAAATTTCAA 
TCGTTGAGTG ATAATGATCT TGCAGTCTGT GCAACCATCA TCGGTATAAT TTCTACTGCT 
ATCGCAGACT CGACTCACGT TAAGCGAATC ACAAAAATAG CACAATCACA AACAATGGCA 
GAGACATATG TTGCATCTTA TGTTGTTCCA CGATTATATT TGAAAGATCA AACAAATCAC 
ATTGAATCTG ACAGTATCCT AAACCTATTA AACATTTTAA CAACTCATCT ATCGCATCCT 
GGCACTAACA AGGATATGAT ATTAAAGTTG GTCTGCCAAG TGACAAAATT CGGTTTACTT 
CTCCAAGTAA GTGCACAAGA AAGGAAAGAT TTCTTGAAAA AAGTCATGGA TACTATACAA 
GACAAATTGA TCAACGATGT GACAGCAATT CAAACGTGGT CATACCTATC ACTATATTCA 
ACAGATTTAG AGAATTCCAG TCTTTTTCAA GAAAAATTAC TTGAAACTAA CGTTTCTAAG 
CAAAACGATT TCTTATTTTC AGTTGGTGAA TCTTTAAGTG TTGTGGCTGG TAAATGGTCA 
AGTAAGTACT TGATCAAACA GATTGATATT CCTAATTTCA ATGTTGAGAT CATGCAACAA 
AAATTCCCTG CTACAAATGT TACCACTATA CTTGATGAGA TCTTTTCAGG TTGCGACTCT 
ACAAAACCCT CTCTGAGGAA GGCATCTTGT ATTTGGCTGT TATCATATAT TCAGTACTTA 
GGTCATTTGC CAGAAGTAAG TTCCAAATGT AATGATATTC ACTTGAGATT TATGAGATTT 
TTGGCAGACA GAGATGAATT TATACAGGAT TCCGCCGCTA GAGGGCTTTC TTTGGTTTAC 
GAAATTGGTG GTTCTGATTT AAAAGAAAGT ATGGTCAAAG GGCTACTTAA ATCATTTACA 
GAATCAACCG CAGGGTCTGC ATCTACAAGT GCTACTGGCA TCTCAGGTTC AGTCTCCGAA 
GAGACCGAGT TATTTGAGCC AGGAGTTTTA AACACTGGAG ATGGGTCCAT TAGCACTTAC 
AAAGATATTT TGAATTTGGC ATCTGAAGTT GGGGACCCCG CACTTGTTTA CAAATTTATG 
TCTCTTGCCA AAAGTTCTGC GCTATGGTCA TCTAGGAAAG GTATCGCATT CGGTCTTGGC 
GCCATTATGT CTAAATCTTC TTTAGAAGAA TTATTACTAA AGGATCAGCA AACTGCCAAG 
AAATTAATTC CAAAATTGTA TAGATACAGA TTTGATCCAT TTCAAGCAGT ATCACGCTCG 
ATGACAGACA TCTGGAATAC TTTAATTCCA GAATCCTCTT TGACGATTTC CCTCTATTTC 
AATGATATTC TTGATGAGCT TTTATGTGGC ATGGCTAATA AAGAATGGAG GGTCAGGGAG 
GCCAGTACTT CAGCTTTATT ACAATTGATT CAATCTCAAC CGCAAGAGAA ATTTTCCGAA 
AAAATGCTGA AAATTTGGAC AATGGCATTC AGAACGATGG ATGATATAAA AGATAGTGTC 
CGTGAAGTTG GTACCAAGTT CACCACTGTA TTGGCCAAAA TTTTGGCAAG ATCTATTGAT 
GTGGAAAAAG GTGTGAACCC TACCAAGTCT AAGGAAATAC TAGACAATAT TTTGCCATTT 
TTATGGGGTC CACATGGTCT GAACAGTGAT GCAGAAGAGG TTAGAAATTT TGCGCTCACA 
ACTTTGATTG ACTTGGTGAA ACACTCCCCT GGAGCCATCA AACCGTTCAC TCCAAAATTA 
ATTTACGATT TTATCACATT GTTTTCTTCC ATTGAACCTC AGGTGATTAA CTATTTGGCA 
TTAAATGCTG CAAATTATAA CATTGATGCT AACGTCATTG ATACGCAAAG GAAAAATGGT 
GTCACAAATT CACCTCTATT CCAGACCATC GAAAAATTAA TTAACAATTC AGATGATTGT 
ATGATGGAAG AAATAATTAA TGTGGTTATT AAAGCTAGCA GAAAATCAGT CGGATTGCCA 
TCTAAGGTCG CATCCTCACT TGTCATAATT ATCTTAGTGA AACGGTACTC TATCGAAATG 
AAACCCTATT CTGGAAAGTT GTTGAAAGTC TGCTTGACTA TGTTTGAAGA TAGAAATGAG 
TCGGTTAATA TAGCATTTGC AATTTCAATG GGATATTTAT TCAAGGTTTC AGCGTTGGAT 
AAGTGTATTA AATACTCTGA AAAACTAATA ACGAAATATT TTGAGCCTAC CTCAACAGAA 
AATAACAAGA AAGTTGTTGG TACAGCGATC GACTCGATTT TGAATTATGC GAAGTCAGAA 
TTCGATAATG TGGCAAGTGT ATTTATGCCT TTGATTTTCA TAGCATGCAA TGATGAAGAT 
AAAGATTTGG AAACCTTATA TAATAAAATC TGGACGGAAG CTTCCAGCTC TGGCGCCGGT 
ACGGTTAAAT TATACCTGCC AGAAATTTTA AACGTACTTT GTGTGAATAT AAAGTCAAAT 
GATTTCTCTA TTCGAAAAAC ATGTGCTAAA TCAGTCATAC AGTTGTGTGG CGGTATTAAT 
GATAGTATTC CTTATCCACA AATTGTTAAG TTATTTGATA TATCGAGGGA GGCATTAAGT 
GGTAGATCAT GGGATGGTAA AGAGCATATA GTGGCAGCAT TGGTTTCATT AACAGAAAAA 
TTTTCTCAAA CTGTGGCCGA CAATAATGAT CTACAAGAAT CAATTAATCA TGTAATGTAC 
ACTGAAGTTT CGAGAAAAAG TATGAAATAT GTTAAGAAGA TACTACCGCT TTACGCGAGA 
TATATCAATG TGAATCCACA AGAAGAGACG ATCACCTTCT TAATTGAAAA AGCCAAAGAA 
ATGATTCGAT TGTTGGGCAG CGAATCTGAT GATAGTGAAG GTTCCATTAA GCAGACTTCC 
GATGAATCCA CAATAAAAAG AATTAAGCCT AACACTGAAA TTACACAAAA GTCATCCAAA 
GAGAACATTG AGAACGAAGA GTATGTGATC AACTTGTTAA AAGTTAGTGT TGACATATGC 
AACAATTCTA AATCGAGGTA TCCGATGAAT TTATTGGAGT TCATTATAGA TGAGATTGCA 
TACCTTTTCC ATAATGACAG AATTATACAC ACATGGAGAA CGCAACTAGC TGCTAGTGAA 
ATTGGTATCT CGATAGTTGG AAGATTTAGT ACCATAAGTA GCGCTGACTT TATCCAAAAT 
GTGGGGAGAC TATGGGATCA AACTTTTCCA ATAAATTGTA ACAAGGAAAC CATTGAGAAT 
GTGAAATTGC AGATGATAAA GTTCGGTGGC CTGATCATTC AGAAGATTCC AAGCTTGCAG 
AACAATATCG AGGAAAACTT ACGGTTGTTG AACAGTATTG ATTCAACGAG CAGAATTGAG 
TTGGAATTGA AAAATATTGG TCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)
Johnston M,Andrews S,Brinkman R,Cooper J,Ding H,Dover J,Du Z,Favello A,Fulton L,Gattung S

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ECM29 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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