MRD1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	MRD1
Entrez Gene ID	856228
Full Name	RNA-binding ribosome biosynthesis protein MRD1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001184209.1	NP_015437.1	RNA-binding ribosome biosynthesis protein MRD1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Rbm19	XP_003751242.1
Kluyveromyces lactis	KLLA0D14949g	XP_453723.1
Eremothecium gossypii	AGOS_ADR035C	NP_984131.2
Arabidopsis thaliana (thale cress)	AT5G08695	NP_001119193.1
Homo sapiens (human)	RBM19	NP_001140170.1
Danio rerio (zebrafish)	rbm19	NP_944597.1
Drosophila melanogaster (fruit fly)	CG3335	NP_648337.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005249	XP_314164.4
Caenorhabditis elegans (roundworm)	rbd-1	NP_502432.1
Schizosaccharomyces pombe (fission yeast)	SPBP22H7.02c	NP_595599.1
Magnaporthe oryzae (rice blast fungus)	MGG_01217	XP_003714116.1
Xenopus tropicalis (tropical clawed frog)	rbm19	NP_001163962.1
	RBM19	XP_001106082.1
Bos taurus (cattle)	RBM19	NP_001039420.1
Saccharomyces cerevisiae (baker's yeast)	MRD1	NP_015437.1
Neurospora crassa	NCU02611	XP_965014.1
Mus musculus (house mouse)	Rbm19	NP_083038.1
Gallus gallus (chicken)	RBM19	NP_001034357.2
Pan troglodytes (chimpanzee)	RBM19	XP_522578.2
Canis lupus familiaris (dog)	RBM19	XP_005636326.1
Arabidopsis thaliana (thale cress)	AT4G19610	NP_193696.3
Oryza sativa (rice)	Os04g0611500	NP_001053839.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
Bussey H, Storms RK, Ahmed A, Albermann K, Allen E, Ansorge W, Araujo R, Aparicio A, Barrell B, Badcock K, Benes V, Botstein D, Bowman S, Br?ckner M, Carpenter J, Cherry JM, Chung E, Churcher C, Coster F, Davis K, Davis RW, Dietrich FS, Delius H, DiPaolo T, Hani J
Nature387(6632 Suppl)103-5(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

conclude that Linker 2 has an essential role for Mrd1p beyond just providing a defined length between RBD2 and RBD3
Title: Linker 2 of the eukaryotic pre-ribosomal processing factor Mrd1p is an essential interdomain functionally coupled to upstream RNA Binding Domain 2 (RBD2).

Mrd1p coordinates key events in biosynthesis of small ribosome subunits.
Title: Mrd1p is required for release of base-paired U3 snoRNA within the preribosomal complex.

Mrd1p is required for establishing productive structures within the 90S preribosomal complex.
Title: Mrd1p binds to pre-rRNA early during transcription independent of U3 snoRNA and is required for compaction of the pre-rRNA into small subunit processomes.

The following MRD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MRD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi05061	NM_001184209.1 Latest version!	Saccharomyces cerevisiae S288C RNA-binding ribosome biosynthesis protein MRD1 (MRD1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MRD1 ( NM_001184209.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_015437.1 Saccharomyces cerevisiae S288C RNA-binding ribosome biosynthesis protein MRD1 (MRD1), partial mRNA.

Price & Availability↑

CloneID	OSi05061
Clone ID Related Accession (Same CDS sequence)	NM_001184209.1
Accession Version	NM_001184209.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2664bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	RNA-binding ribosome biosynthesis protein MRD1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001148). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCCCGTA TCATTGTCAA GGGTTTGCCC GTCTATCTAA CAGATGATAA TCTCAGGGAG 
CATTTTACAA AAAGATTACG TCAAAAACAT TCCCATCAAG CAGTTAATGG TAGTGGTCCA 
GACCTCATAA CAGATGTTAA AATTCTTAGA GATAGGAACG GAGAAAGTAG AAGATTTGGA 
TTTATCGGAT ACCGTAACGA GGAAGACGCT TTTGATGCTG TAGAGTATTT CAATGGCAGT 
TTTATCAACA CTTCTAAGAT AGAAGTGTCC ATGGCTAAAA GCTTTGCAGA CCCAAGAGTC 
CCGCAGCCCA TGAAGGAGAA GAGAAGAGAA GCTCTGAAAA GGTTTAGGGA AAAGGAAGAA 
AAATTGCTAC AAGAAGAAAA TCGAAAGAAA AAGAAAGTGG ATGAAAATAA GCACAGCAAC 
ATTGATGACG AGATTCGCAA GAACAAGCAA TTACAAGAGT TCATGGAGAC TATGAAGCCA 
AGCTCACAAG TCACATCATG GGAAAAAGTT GGCATTGACA AGAGCATAGA GGACGAAAAA 
CTAAAACGGG AAGAAGAGGA CTCCAGCGTG CAAGGGAATT CTCTATTGGC TCATGCATTA 
GCCTTAAAGG AAGAGAATAA TAAAGACGAA GCACCAAATT TGGTCATTGA GAATGAAAGT 
GACGATGAAT ATTCGGCTCT CAACAGAAAC AGGGATGAAG ATCAAGAAGA TGCCGGAGAG 
GAAGAAAAGA TGATAAGTAT TAGCAATTTG AAGGATACTG ATATAGGTTT AGTTAATGAT 
GACGCTAATA GTGATGAAAA AGAGAATGAA AAGAGGAGAA ACCTCGCCCA AGATGAAAAG 
GTTTCTGACC TAGATTGGTT CAAACAACGC CGTGTAAGAA TCAAAGAAAG TGAAGCGGAA 
ACAAGGGAAA AATCATCATC ATACGCTACT GAACAAAATG AAAGTCTCGA TACCAAGAAG 
GAAGAACAAC CAGAACGTGC TGTGCCGCAA AAGACGGACG AAGAACTGGC TATTGAAAAA 
ATTAACCAAA CTGGCCGTTT ATTCTTACGT AATATCTTGT ATACCTCCAA AGAAGAAGAT 
TTCAGGAAAC TATTTAGTCC ATTTGGAGAG TTAGAGGAGG TTCATGTGGC TTTGGATACC 
AGGACTGGTC AATCCAAAGG ATTTGCATAC GTTCTATTTA AGGATTCTAA GAATGCAGTG 
AACGCCTATG TTGAATTGGA TAAACAGATT TTTCAAGGAA GACTATTGCA TATTCTTCCT 
GGTGAAGAAA AAAAAAGCCA TAGATTAGAT GAGTTTGATT TAAAGAATAT GCCATTGAAA 
AAGCAAAAGG AATTGAAAAG GAAGGCAGCT GCATCCAGAC AAACATTTTC TTGGAACTCC 
TTGTATATGA ACCAAGATGC TGTTTTGGGT AGTGTGGCAG CCAAATTGGG TCTAGAAAAA 
TCTCAATTGA TCGATGCTGA AAACTCAAGT TCGGCTGTCA AACAAGCTTT GGCTGAAGCC 
CATGTTATTG GTGATGTTAG AAAATACTTT GAATCCAAAG GTGTTGATCT GACCAAGTTT 
TCACAGTTAA AATCTACAAA TCAAAGAGAC GATAAAGTGA TACTGGTTAA AAATTTTCCT 
TTTGGGACAA CTAGAGAAGA GCTTGGCGAA ATGTTTTTGC CATATGGTAA ATTGGAAAGA 
TTGCTCATGC CACCAGCTGG TACAATAGCT ATAGTGCAAT TCAGGGACAC AACGTCAGCA 
AGGGCAGCTT TCACTAAACT ATCGTATAAA AGATTTAAGG ACGGCATAAT ATATCTAGAA 
AGGGGGCCTA AGGATTGCTT TACTAAACCA GCTGAGGCCG ATGATTTGAT AAATAACACT 
TCTGCAAAAG AAGAAGAAAA CCCGGTAGAG GTAAAACCTT CATCAAATGA CTTAATGGAA 
GCAAACAAGG ATGTAACTGA AGGGTCATCT AATGCTCATG ATGAAGACGT TATTGATGGT 
CCAACTGTCT CAATTTTCAT AAAGAATCTA AACTTCTCCA CGACGAACCA AAATCTTACT 
GATAGGTTCA AGGTTTTCAC TGGTTTTGTT GTTGCCCAAG TGAAGACAAA ACCTGATCCA 
AAACATCAAG GCAAGACCTT GTCAATGGGG TTTGGTTTTG TAGAATTCAG GACCAAGGAA 
CAAGCTAATG CCGTGATAGC TGCAATGGAT GGGACGGTAA TCGACGGCCA TAAGATTCAA 
TTGAAGTTAT CCCATAGACA AGCATCTCAG AGCGGTAATA CAAAGACTAA ATCCAATAAG 
AAAAGCGGTA AAATCATTGT TAAGAATCTT CCATTTGAAG CTACTAGGAA AGACGTTTTC 
GAATTATTCA ATTCTTTTGG TCAATTAAAA TCTGTTAGAG TTCCAAAGAA GTTCGACAAG 
TCTGCAAGAG GTTTTGCGTT CGTGGAATTT TTACTTCCAA AGGAAGCAGA AAATGCTATG 
GATCAGTTGC ACGGTGTCCA TTTACTGGGC CGCAGATTGG TAATGCAATA TGCAGAAGAA 
GATGCAGTTG ACGCTGAAGA AGAGATAGCC AGGATGACAA AGAAGGTTAG AAAGCAAGTT 
GCCACTAATG AGATGGCTGC CTTAAGAAAT GGGGGCGGTA GAAAGAAACT GGATGTGGAT 
GATGAAGAAA ACGAAGGTTT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_015437.1
CDS	1..2664
Translation	MSRIIVKGLPVYLTDDNLREHFTKRLRQKHSHQAVNGSGPDLITDVKILRDRNGESRRFGFIGYRNEEDAFDAVEYFNGSFINTSKIEVSMAKSFADPRVPQPMKEKRREALKRFREKEEKLLQEENRKKKKVDENKHSNIDDEIRKNKQLQEFMETMKPSSQVTSWEKVGIDKSIEDEKLKREEEDSSVQGNSLLAHALALKEENNKDEAPNLVIENESDDEYSALNRNRDEDQEDAGEEEKMISISNLKDTDIGLVNDDANSDEKENEKRRNLAQDEKVSDLDWFKQRRVRIKESEAETREKSSSYATEQNESLDTKKEEQPERAVPQKTDEELAIEKINQTGRLFLRNILYTSKEEDFRKLFSPFGELEEVHVALDTRTGQSKGFAYVLFKDSKNAVNAYVELDKQIFQGRLLHILPGEEKKSHRLDEFDLKNMPLKKQKELKRKAAASRQTFSWNSLYMNQDAVLGSVAAKLGLEKSQLIDAENSSSAVKQALAEAHVIGDVRKYFESKGVDLTKFSQLKSTNQRDDKVILVKNFPFGTTREELGEMFLPYGKLERLLMPPAGTIAIVQFRDTTSARAAFTKLSYKRFKDGIIYLERGPKDCFTKPAEADDLINNTSAKEEENPVEVKPSSNDLMEANKDVTEGSSNAHDEDVIDGPTVSIFIKNLNFSTTNQNLTDRFKVFTGFVVAQVKTKPDPKHQGKTLSMGFGFVEFRTKEQANAVIAAMDGTVIDGHKIQLKLSHRQASQSGNTKTKSNKKSGKIIVKNLPFEATRKDVFELFNSFGQLKSVRVPKKFDKSARGFAFVEFLLPKEAENAMDQLHGVHLLGRRLVMQYAEEDAVDAEEEIARMTKKVRKQVATNEMAALRNGGGRKKLDVDDEENEGF

Target ORF information:

RefSeq Version	NM_001184209.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C RNA-binding ribosome biosynthesis protein MRD1 (MRD1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001184209.1

ORF Insert Sequence:

ATGTCCCGTA TCATTGTCAA GGGTTTGCCC GTCTATCTAA CAGATGATAA TCTCAGGGAG 
CATTTTACAA AAAGATTACG TCAAAAACAT TCCCATCAAG CAGTTAATGG TAGTGGTCCA 
GACCTCATAA CAGATGTTAA AATTCTTAGA GATAGGAACG GAGAAAGTAG AAGATTTGGA 
TTTATCGGAT ACCGTAACGA GGAAGACGCT TTTGATGCTG TAGAGTATTT CAATGGCAGT 
TTTATCAACA CTTCTAAGAT AGAAGTGTCC ATGGCTAAAA GCTTTGCAGA CCCAAGAGTC 
CCGCAGCCCA TGAAGGAGAA GAGAAGAGAA GCTCTGAAAA GGTTTAGGGA AAAGGAAGAA 
AAATTGCTAC AAGAAGAAAA TCGAAAGAAA AAGAAAGTGG ATGAAAATAA GCACAGCAAC 
ATTGATGACG AGATTCGCAA GAACAAGCAA TTACAAGAGT TCATGGAGAC TATGAAGCCA 
AGCTCACAAG TCACATCATG GGAAAAAGTT GGCATTGACA AGAGCATAGA GGACGAAAAA 
CTAAAACGGG AAGAAGAGGA CTCCAGCGTG CAAGGGAATT CTCTATTGGC TCATGCATTA 
GCCTTAAAGG AAGAGAATAA TAAAGACGAA GCACCAAATT TGGTCATTGA GAATGAAAGT 
GACGATGAAT ATTCGGCTCT CAACAGAAAC AGGGATGAAG ATCAAGAAGA TGCCGGAGAG 
GAAGAAAAGA TGATAAGTAT TAGCAATTTG AAGGATACTG ATATAGGTTT AGTTAATGAT 
GACGCTAATA GTGATGAAAA AGAGAATGAA AAGAGGAGAA ACCTCGCCCA AGATGAAAAG 
GTTTCTGACC TAGATTGGTT CAAACAACGC CGTGTAAGAA TCAAAGAAAG TGAAGCGGAA 
ACAAGGGAAA AATCATCATC ATACGCTACT GAACAAAATG AAAGTCTCGA TACCAAGAAG 
GAAGAACAAC CAGAACGTGC TGTGCCGCAA AAGACGGACG AAGAACTGGC TATTGAAAAA 
ATTAACCAAA CTGGCCGTTT ATTCTTACGT AATATCTTGT ATACCTCCAA AGAAGAAGAT 
TTCAGGAAAC TATTTAGTCC ATTTGGAGAG TTAGAGGAGG TTCATGTGGC TTTGGATACC 
AGGACTGGTC AATCCAAAGG ATTTGCATAC GTTCTATTTA AGGATTCTAA GAATGCAGTG 
AACGCCTATG TTGAATTGGA TAAACAGATT TTTCAAGGAA GACTATTGCA TATTCTTCCT 
GGTGAAGAAA AAAAAAGCCA TAGATTAGAT GAGTTTGATT TAAAGAATAT GCCATTGAAA 
AAGCAAAAGG AATTGAAAAG GAAGGCAGCT GCATCCAGAC AAACATTTTC TTGGAACTCC 
TTGTATATGA ACCAAGATGC TGTTTTGGGT AGTGTGGCAG CCAAATTGGG TCTAGAAAAA 
TCTCAATTGA TCGATGCTGA AAACTCAAGT TCGGCTGTCA AACAAGCTTT GGCTGAAGCC 
CATGTTATTG GTGATGTTAG AAAATACTTT GAATCCAAAG GTGTTGATCT GACCAAGTTT 
TCACAGTTAA AATCTACAAA TCAAAGAGAC GATAAAGTGA TACTGGTTAA AAATTTTCCT 
TTTGGGACAA CTAGAGAAGA GCTTGGCGAA ATGTTTTTGC CATATGGTAA ATTGGAAAGA 
TTGCTCATGC CACCAGCTGG TACAATAGCT ATAGTGCAAT TCAGGGACAC AACGTCAGCA 
AGGGCAGCTT TCACTAAACT ATCGTATAAA AGATTTAAGG ACGGCATAAT ATATCTAGAA 
AGGGGGCCTA AGGATTGCTT TACTAAACCA GCTGAGGCCG ATGATTTGAT AAATAACACT 
TCTGCAAAAG AAGAAGAAAA CCCGGTAGAG GTAAAACCTT CATCAAATGA CTTAATGGAA 
GCAAACAAGG ATGTAACTGA AGGGTCATCT AATGCTCATG ATGAAGACGT TATTGATGGT 
CCAACTGTCT CAATTTTCAT AAAGAATCTA AACTTCTCCA CGACGAACCA AAATCTTACT 
GATAGGTTCA AGGTTTTCAC TGGTTTTGTT GTTGCCCAAG TGAAGACAAA ACCTGATCCA 
AAACATCAAG GCAAGACCTT GTCAATGGGG TTTGGTTTTG TAGAATTCAG GACCAAGGAA 
CAAGCTAATG CCGTGATAGC TGCAATGGAT GGGACGGTAA TCGACGGCCA TAAGATTCAA 
TTGAAGTTAT CCCATAGACA AGCATCTCAG AGCGGTAATA CAAAGACTAA ATCCAATAAG 
AAAAGCGGTA AAATCATTGT TAAGAATCTT CCATTTGAAG CTACTAGGAA AGACGTTTTC 
GAATTATTCA ATTCTTTTGG TCAATTAAAA TCTGTTAGAG TTCCAAAGAA GTTCGACAAG 
TCTGCAAGAG GTTTTGCGTT CGTGGAATTT TTACTTCCAA AGGAAGCAGA AAATGCTATG 
GATCAGTTGC ACGGTGTCCA TTTACTGGGC CGCAGATTGG TAATGCAATA TGCAGAAGAA 
GATGCAGTTG ACGCTGAAGA AGAGATAGCC AGGATGACAA AGAAGGTTAG AAAGCAAGTT 
GCCACTAATG AGATGGCTGC CTTAAGAAAT GGGGGCGGTA GAAAGAAACT GGATGTGGAT 
GATGAAGAAA ACGAAGGTTT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
Nature387(6632 Suppl)103-5(1997 May)
Bussey H,Storms RK,Ahmed A,Albermann K,Allen E,Ansorge W,Araujo R,Aparicio A,Barrell B,Badcock K,Benes V,Botstein D,Bowman S,Br?ckner M,Carpenter J,Cherry JM,Chung E,Churcher C,Coster F,Davis K,Davis RW,Dietrich FS,Delius H,DiPaolo T,Hani J

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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MRD1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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