PUF2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	PUF2
Entrez Gene ID	856155
Full Name	Puf2p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001184139.1	NP_015367.1	Puf2p

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
Bussey H, Storms RK, Ahmed A, Albermann K, Allen E, Ansorge W, Araujo R, Aparicio A, Barrell B, Badcock K, Benes V, Botstein D, Bowman S, Br?ckner M, Carpenter J, Cherry JM, Chung E, Churcher C, Coster F, Davis K, Davis RW, Dietrich FS, Delius H, DiPaolo T, Hani J
Nature387(6632 Suppl)103-5(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that pumilio/fem-3 mRNA binding factor (PUF) protein Puf2p specifically binds more than 1,000 mRNAs, which contain multiple iterations of UAAU-binding elements.
Title: Target selection by natural and redesigned PUF proteins.

Puf1p also binds the dual UAAU sequence, consistent with the prior observation that it associates with similar populations of mRNAs
Title: Divergent RNA binding specificity of yeast Puf2p.

The following PUF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PUF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi05002	NM_001184139.1 Latest version!	Saccharomyces cerevisiae S288C Puf2p (PUF2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PUF2 ( NM_001184139.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_015367.1 Saccharomyces cerevisiae S288C Puf2p (PUF2), partial mRNA.

Price & Availability↑

CloneID	OSi05002
Clone ID Related Accession (Same CDS sequence)	NM_001184139.1
Accession Version	NM_001184139.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Puf2p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001148). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGACAACA AAAGACTGTA TAACGGTAAC CTATCCAACA TTCCTGAGGT TATAGATCCT 
GGAATTACAA TTCCAATTTA TGAAGAGGAT ATTAGAAATG ATACACGCAT GAATACAAAT 
GCTCGTTCTG TGAGAGTCTC TGACAAGAGA GGTCGTTCTA GCTCCACTTC CCCACAGAAA 
ATTGGCTCCT ATCGTACTCG TGCGGGCAGG TTTTCAGATA CTCTAACCAA TCTACTCCCT 
TCGATTAGCG CTAAGCTACA CCATTCAAAG AAGAGTACCC CTGTAGTTGT TGTGCCTCCT 
ACGAGCAGTA CTCCAGACAG TTTAAATTCC ACGACATACG CTCCCAGAGT GTCTAGCGAT 
TCTTTTACGG TGGCTACTCC GCTGTCATTA CAGTCTACTA CGACCAGAAC AAGAACAAGA 
AATAACACAG TATCTTCTCA GATCACTGCA AGTTCTTCTT TGACTACTGA TGTGGGCAAT 
GCCACGAGTG CAAATATTTG GAGTGCTAAT GCAGAAAGTA ATACTTCTTC CAGTCCTTTG 
TTTGATTATC CTCTCGCCAC TTCTTATTTT GAGCCATTGA CTAGATTCAA GTCCACTGAT 
AATTATACGC TGCCTCAAAC AGCTCAACTA AACAGTTTTC TGGAGAAAAA TGGGAATCCA 
AACATATGGT CGAGTGCAGG AAATAGTAAC ACTGACCACC TTAATACTCC GATAGTTAAC 
AGGCAGCGCT CTCAATCTCA ATCTACCACA AACAGGGTTT ATACTGACGC GCCCTACTAT 
CAACAGCCTG CACAGAATTA CCAGGTTCAA GTGCCTCCTA GAGTTCCAAA AAGTACTTCA 
ATATCCCCTG TTATCCTTGA TGACGTCGAT CCAGCCTCGA TAAATTGGAT TACAGCAAAC 
CAAAAAGTAC CACTAGTCAA TCAAATATCG GCGCTTTTGC CCACTAACAC AATATCTATT 
TCAAACGTGT TTCCATTACA GCCTACTCAA CAACATCAAC AGAACGCTGT AAATTTGACG 
AGTACTTCCT TAGCGACTTT ATGTTCACAG TATGGTAAAG TGCTTTCTGC GAGGACACTA 
AGAGGCCTGA ATATGGCATT AGTAGAGTTT TCCACAGTTG AAAGTGCAAT ATGTGCTTTG 
GAAGCTCTAC AAGGTAAGGA ACTGTCTAAA GTAGGGGCAC CAAGTACGGT ATCTTTTGCG 
CGAGTCTTAC CCATGTATGA ACAACCTCTT AACGTTAATG GCTTCAACAA CACTCCCAAG 
CAACCGCTGT TGCAAGAACA GTTGAATCAC GGGGTGCTCA ATTATCAGCT GCAACAATCG 
CTACAACAGC CGGAGCTACA ACAACAACCA ACTTCATTTA ATCAACCAAA TCTTACATAT 
TGTAATCCCA CTCAGAATTT GAGCCATCTG CAATTATCTT CAAACGAAAA TGAACCTTAC 
CCTTTCCCGT TGCCTCCACC ATCATTATCG GATAGTAAAA AGGATATATT ACACACTATA 
AGTTCTTTTA AGTTAGAATA TGATCATCTA GAGTTGAATC ATCTCTTGCA GAATGCCTTG 
AAAAATAAAG GAGTGTCTGA TACAAATTAT TTTGGTCCCC TGCCTGAGCA CAACTCCAAA 
GTTCCAAAGA GAAAGGATAC GTTCGATGCA CCAAAATTGC GGGAACTGAG AAAGCAGTTT 
GACTCGAACT CATTATCTAC TATTGAAATG GAGCAATTGG CAATCGTCAT GCTGGACCAA 
TTACCAGAAT TAAGTTCAGA CTATTTGGGT AATACCGTTA TTCAAAAATT GTTTGAAAAC 
TCGTCAAATA TCATCAGGGA TATTATGTTG AGGAAGTGTA ATAAATATCT CACATCTATG 
GGTGTTCACA AAAACGGTAC TTGGGTTTGT CAGAAGATCA TCAAAATGGC AAACACGCCA 
AGGCAGATAA ATCTTGTAAC TTCAGGTGTA AGCGATTACT GTACTCCACT CTTCAACGAC 
CAGTTTGGAA ATTATGTTAT TCAAGGTATT TTAAAGTTTG GTTTTCCCTG GAATAGTTTC 
ATTTTTGAAA GCGTTTTATC TCATTTTTGG ACAATAGTTC AAAACAGGTA TGGATCTCGT 
GCTGTAAGGG CTTGTCTAGA AGCAGATTCC ATTATTACCC AATGCCAATT GCTGACTATC 
ACTTCACTGA TAATTGTGCT TTCTCCTTAT CTAGCTACGG ATACGAATGG CACTTTACTG 
ATCACTTGGC TATTGGATAC TTGCACTTTA CCTAACAAGA ACTTGATTTT ATGTGATAAA 
CTGGTAAATA AAAATCTGGT TAAACTATGT TGTCATAAAT TAGGCTCTCT TACTGTCTTA 
AAGATTCTCA ATTTAAGAGG TGGTGAAGAA GAGGCACTGT CGAAGAATAA AATTATCCAT 
GCAATATTTG ATGGTCCAAT TTCTAGTGAT TCTATCTTGT TCCAGATACT AGATGAGGGT 
AATTATGGTC CAACATTTAT TTATAAGGTT TTAACCTCGA GAATTCTTGA CAATAGCGTT 
AGAGATGAAG CTATAACAAA GATTCGTCAA CTGATTTTAA ATTCCAATAT CAATTTACAA 
AGTCGCCAGT TACTGGAGGA AGTTGGGCTA TCATCAGCAG GAATATCACC CAAACAATCC 
TCCAAGAATC ATCGTAAACA GCATCCTCAG GGGTTTCATT CTCCTGGTCG CGCCAGAGGC 
GTTTCTGTTT CCAGTGTGAG AAGTTCAAAT TCAAGGCATA ATAGCGTTAT ACAAATGAAC 
AATGCAGGTC CAACGCCAGC ATTGAACTTT AACCCAGCCC CGATGAGTGA AATAAATTCC 
TACTTCAACA ATCAACAAGT GGTGTACTCC GGAAATCAAA ATCAAAATCA GAACGGTAAT 
TCTAACGGAT TAGATGAATT GAATTCACAA TTTGATTCAT TTAGAATTGC TAATGGAACA 
AATTTATCCC TACCTATAGT TAACTTACCA AATGTAAGTA ATAATAATAA CAATTATAAT 
AATTCTGGTT ATTCGAGTCA AATGAATCCT CTAAGCCGCT CAGTGAGTCA TAATAACAAT 
AATAATACCA ATAACTACAA CAATAATGAT AATGATAACA ATAATAATAA CAATAACAAT 
AATAATAATA ATAATAATAA TAATAATAAT AATAATAATA GTAATAATAG TAATAATAAT 
AATAATAATG ATACAAGTTT GTACCGCTAC AGGTCATACG GATATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_015367.1
CDS	1..3228
Translation	MDNKRLYNGNLSNIPEVIDPGITIPIYEEDIRNDTRMNTNARSVRVSDKRGRSSSTSPQKIGSYRTRAGRFSDTLTNLLPSISAKLHHSKKSTPVVVVPPTSSTPDSLNSTTYAPRVSSDSFTVATPLSLQSTTTRTRTRNNTVSSQITASSSLTTDVGNATSANIWSANAESNTSSSPLFDYPLATSYFEPLTRFKSTDNYTLPQTAQLNSFLEKNGNPNIWSSAGNSNTDHLNTPIVNRQRSQSQSTTNRVYTDAPYYQQPAQNYQVQVPPRVPKSTSISPVILDDVDPASINWITANQKVPLVNQISALLPTNTISISNVFPLQPTQQHQQNAVNLTSTSLATLCSQYGKVLSARTLRGLNMALVEFSTVESAICALEALQGKELSKVGAPSTVSFARVLPMYEQPLNVNGFNNTPKQPLLQEQLNHGVLNYQLQQSLQQPELQQQPTSFNQPNLTYCNPTQNLSHLQLSSNENEPYPFPLPPPSLSDSKKDILHTISSFKLEYDHLELNHLLQNALKNKGVSDTNYFGPLPEHNSKVPKRKDTFDAPKLRELRKQFDSNSLSTIEMEQLAIVMLDQLPELSSDYLGNTVIQKLFENSSNIIRDIMLRKCNKYLTSMGVHKNGTWVCQKIIKMANTPRQINLVTSGVSDYCTPLFNDQFGNYVIQGILKFGFPWNSFIFESVLSHFWTIVQNRYGSRAVRACLEADSIITQCQLLTITSLIIVLSPYLATDTNGTLLITWLLDTCTLPNKNLILCDKLVNKNLVKLCCHKLGSLTVLKILNLRGGEEEALSKNKIIHAIFDGPISSDSILFQILDEGNYGPTFIYKVLTSRILDNSVRDEAITKIRQLILNSNINLQSRQLLEEVGLSSAGISPKQSSKNHRKQHPQGFHSPGRARGVSVSSVRSSNSRHNSVIQMNNAGPTPALNFNPAPMSEINSYFNNQQVVYSGNQNQNQNGNSNGLDELNSQFDSFRIANGTNLSLPIVNLPNVSNNNNNYNNSGYSSQMNPLSRSVSHNNNNNTNNYNNNDNDNNNNNNNNNNNNNNNNNNNNNSNNSNNNNNNDTSLYRYRSYGY

Target ORF information:

RefSeq Version	NM_001184139.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Puf2p (PUF2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001184139.1

ORF Insert Sequence:

ATGGACAACA AAAGACTGTA TAACGGTAAC CTATCCAACA TTCCTGAGGT TATAGATCCT 
GGAATTACAA TTCCAATTTA TGAAGAGGAT ATTAGAAATG ATACACGCAT GAATACAAAT 
GCTCGTTCTG TGAGAGTCTC TGACAAGAGA GGTCGTTCTA GCTCCACTTC CCCACAGAAA 
ATTGGCTCCT ATCGTACTCG TGCGGGCAGG TTTTCAGATA CTCTAACCAA TCTACTCCCT 
TCGATTAGCG CTAAGCTACA CCATTCAAAG AAGAGTACCC CTGTAGTTGT TGTGCCTCCT 
ACGAGCAGTA CTCCAGACAG TTTAAATTCC ACGACATACG CTCCCAGAGT GTCTAGCGAT 
TCTTTTACGG TGGCTACTCC GCTGTCATTA CAGTCTACTA CGACCAGAAC AAGAACAAGA 
AATAACACAG TATCTTCTCA GATCACTGCA AGTTCTTCTT TGACTACTGA TGTGGGCAAT 
GCCACGAGTG CAAATATTTG GAGTGCTAAT GCAGAAAGTA ATACTTCTTC CAGTCCTTTG 
TTTGATTATC CTCTCGCCAC TTCTTATTTT GAGCCATTGA CTAGATTCAA GTCCACTGAT 
AATTATACGC TGCCTCAAAC AGCTCAACTA AACAGTTTTC TGGAGAAAAA TGGGAATCCA 
AACATATGGT CGAGTGCAGG AAATAGTAAC ACTGACCACC TTAATACTCC GATAGTTAAC 
AGGCAGCGCT CTCAATCTCA ATCTACCACA AACAGGGTTT ATACTGACGC GCCCTACTAT 
CAACAGCCTG CACAGAATTA CCAGGTTCAA GTGCCTCCTA GAGTTCCAAA AAGTACTTCA 
ATATCCCCTG TTATCCTTGA TGACGTCGAT CCAGCCTCGA TAAATTGGAT TACAGCAAAC 
CAAAAAGTAC CACTAGTCAA TCAAATATCG GCGCTTTTGC CCACTAACAC AATATCTATT 
TCAAACGTGT TTCCATTACA GCCTACTCAA CAACATCAAC AGAACGCTGT AAATTTGACG 
AGTACTTCCT TAGCGACTTT ATGTTCACAG TATGGTAAAG TGCTTTCTGC GAGGACACTA 
AGAGGCCTGA ATATGGCATT AGTAGAGTTT TCCACAGTTG AAAGTGCAAT ATGTGCTTTG 
GAAGCTCTAC AAGGTAAGGA ACTGTCTAAA GTAGGGGCAC CAAGTACGGT ATCTTTTGCG 
CGAGTCTTAC CCATGTATGA ACAACCTCTT AACGTTAATG GCTTCAACAA CACTCCCAAG 
CAACCGCTGT TGCAAGAACA GTTGAATCAC GGGGTGCTCA ATTATCAGCT GCAACAATCG 
CTACAACAGC CGGAGCTACA ACAACAACCA ACTTCATTTA ATCAACCAAA TCTTACATAT 
TGTAATCCCA CTCAGAATTT GAGCCATCTG CAATTATCTT CAAACGAAAA TGAACCTTAC 
CCTTTCCCGT TGCCTCCACC ATCATTATCG GATAGTAAAA AGGATATATT ACACACTATA 
AGTTCTTTTA AGTTAGAATA TGATCATCTA GAGTTGAATC ATCTCTTGCA GAATGCCTTG 
AAAAATAAAG GAGTGTCTGA TACAAATTAT TTTGGTCCCC TGCCTGAGCA CAACTCCAAA 
GTTCCAAAGA GAAAGGATAC GTTCGATGCA CCAAAATTGC GGGAACTGAG AAAGCAGTTT 
GACTCGAACT CATTATCTAC TATTGAAATG GAGCAATTGG CAATCGTCAT GCTGGACCAA 
TTACCAGAAT TAAGTTCAGA CTATTTGGGT AATACCGTTA TTCAAAAATT GTTTGAAAAC 
TCGTCAAATA TCATCAGGGA TATTATGTTG AGGAAGTGTA ATAAATATCT CACATCTATG 
GGTGTTCACA AAAACGGTAC TTGGGTTTGT CAGAAGATCA TCAAAATGGC AAACACGCCA 
AGGCAGATAA ATCTTGTAAC TTCAGGTGTA AGCGATTACT GTACTCCACT CTTCAACGAC 
CAGTTTGGAA ATTATGTTAT TCAAGGTATT TTAAAGTTTG GTTTTCCCTG GAATAGTTTC 
ATTTTTGAAA GCGTTTTATC TCATTTTTGG ACAATAGTTC AAAACAGGTA TGGATCTCGT 
GCTGTAAGGG CTTGTCTAGA AGCAGATTCC ATTATTACCC AATGCCAATT GCTGACTATC 
ACTTCACTGA TAATTGTGCT TTCTCCTTAT CTAGCTACGG ATACGAATGG CACTTTACTG 
ATCACTTGGC TATTGGATAC TTGCACTTTA CCTAACAAGA ACTTGATTTT ATGTGATAAA 
CTGGTAAATA AAAATCTGGT TAAACTATGT TGTCATAAAT TAGGCTCTCT TACTGTCTTA 
AAGATTCTCA ATTTAAGAGG TGGTGAAGAA GAGGCACTGT CGAAGAATAA AATTATCCAT 
GCAATATTTG ATGGTCCAAT TTCTAGTGAT TCTATCTTGT TCCAGATACT AGATGAGGGT 
AATTATGGTC CAACATTTAT TTATAAGGTT TTAACCTCGA GAATTCTTGA CAATAGCGTT 
AGAGATGAAG CTATAACAAA GATTCGTCAA CTGATTTTAA ATTCCAATAT CAATTTACAA 
AGTCGCCAGT TACTGGAGGA AGTTGGGCTA TCATCAGCAG GAATATCACC CAAACAATCC 
TCCAAGAATC ATCGTAAACA GCATCCTCAG GGGTTTCATT CTCCTGGTCG CGCCAGAGGC 
GTTTCTGTTT CCAGTGTGAG AAGTTCAAAT TCAAGGCATA ATAGCGTTAT ACAAATGAAC 
AATGCAGGTC CAACGCCAGC ATTGAACTTT AACCCAGCCC CGATGAGTGA AATAAATTCC 
TACTTCAACA ATCAACAAGT GGTGTACTCC GGAAATCAAA ATCAAAATCA GAACGGTAAT 
TCTAACGGAT TAGATGAATT GAATTCACAA TTTGATTCAT TTAGAATTGC TAATGGAACA 
AATTTATCCC TACCTATAGT TAACTTACCA AATGTAAGTA ATAATAATAA CAATTATAAT 
AATTCTGGTT ATTCGAGTCA AATGAATCCT CTAAGCCGCT CAGTGAGTCA TAATAACAAT 
AATAATACCA ATAACTACAA CAATAATGAT AATGATAACA ATAATAATAA CAATAACAAT 
AATAATAATA ATAATAATAA TAATAATAAT AATAATAATA GTAATAATAG TAATAATAAT 
AATAATAATG ATACAAGTTT GTACCGCTAC AGGTCATACG GATATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
Nature387(6632 Suppl)103-5(1997 May)
Bussey H,Storms RK,Ahmed A,Albermann K,Allen E,Ansorge W,Araujo R,Aparicio A,Barrell B,Badcock K,Benes V,Botstein D,Bowman S,Br?ckner M,Carpenter J,Cherry JM,Chung E,Churcher C,Coster F,Davis K,Davis RW,Dietrich FS,Delius H,DiPaolo T,Hani J

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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PUF2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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