ORF » Species Summary » Saccharomyces cerevisiae S288C » RPA135 cDNA ORF clone

RPA135 cDNA ORF clone, Saccharomyces cerevisiae S288C

The following RPA135 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RPA135 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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OSi04973 NM_001184107.1
Latest version!		 Saccharomyces cerevisiae S288C DNA-directed RNA polymerase I core subunit RPA135 (RPA135), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    RPA135 ( NM_001184107.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_015335.1 Saccharomyces cerevisiae S288C DNA-directed RNA polymerase I core subunit RPA135 (RPA135), partial mRNA.
    Price & Availability↑
    CloneID OSi04973
    Clone ID Related Accession (Same CDS sequence) NM_001184107.1
    Accession Version NM_001184107.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3612bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product DNA-directed RNA polymerase I core subunit RPA135
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001148). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGCAAAG TGATTAAGCC GCCTGGCCAA GCCAGAACTG CTGATTTCCG TACCTTAGAA 
    CGTGAATCTA GGTTCATCAA TCCACCTAAG GATAAATCAG CATTTCCACT ATTACAGGAA 
    GCTGTTCAAC CTCATATTGG GTCTTTTAAC GCATTAACAG AAGGTCCGGA TGGAGGTTTG 
    TTAAATCTTG GTGTTAAAGA CATCGGTGAA AAAGTTATAT TTGATGGAAA ACCACTCAAT 
    TCTGAAGACG AAATATCCAA CAGCGGATAC CTCGGGAATA AGCTTTCTGT TAGTGTGGAA 
    CAAGTGTCTA TTGCGAAGCC AATGTCTAAC GATGGTGTTT CTTCTGCAGT AGAGAGAAAA 
    GTTTACCCAA GTGAGTCAAG ACAAAGGCTT ACTTCTTACA GAGGTAAATT ACTTTTAAAG 
    TTGAAGTGGT CCGTGAACAA TGGCGAGGAG AACTTATTTG AAGTAAGAGA TTGTGGTGGT 
    CTACCAGTTA TGCTACAGAG TAACAGATGT CACTTGAACA AGATGTCACC ATATGAACTG 
    GTACAACACA AGGAAGAATC CGATGAAATA GGTGGTTATT TCATTGTTAA CGGTATAGAA 
    AAGCTTATTA GAATGCTGAT TGTGCAACGT AGAAATCATC CTATGGCTAT CATCAGACCT 
    TCCTTCGCTA ACAGGGGTGC ATCCTATTCT CATTACGGTA TTCAAATTAG ATCTGTCAGG 
    CCGGATCAAA CTTCCCAAAC TAATGTTTTG CATTACTTAA ATGACGGTCA AGTTACATTT 
    AGATTTTCTT GGAGAAAAAA TGAATATTTA GTTCCTGTTG TTATGATTTT AAAAGCCTTG 
    TGTCATACCA GCGATAGGGA AATTTTTGAT GGTATTATTG GTAATGACGT TAAGGATTCC 
    TTTTTGACCG ATCGTTTAGA ATTACTGTTA CGTGGTTTTA AGAAAAGATA TCCTCATTTA 
    CAAAATCGCA CACAAGTCTT GCAATATTTA GGAGACAAAT TTCGTGTTGT CTTTCAAGCT 
    TCCCCTGATC AATCTGATTT AGAAGTCGGA CAAGAAGTCC TTGATCGCAT CGTTCTCGTG 
    CATTTGGGTA AGGATGGCAG CCAGGATAAA TTCAGAATGT TATTGTTCAT GATCAGAAAG 
    TTGTATTCCT TGGTTGCAGG CGAATGTTCT CCAGATAACC CAGATGCTAC GCAACATCAA 
    GAAGTTCTTT TGGGTGGTTT CTTATATGGT ATGATCCTAA AAGAAAAGAT TGATGAGTAC 
    TTACAGAATA TTATTGCTCA AGTCAGGATG GATATTAACC GTGGTATGGC CATTAATTTC 
    AAAGACAAAA GGTACATGTC AAGAGTTTTA ATGAGGGTTA ATGAAAACAT TGGTTCCAAA 
    ATGCAATACT TTTTATCAAC TGGTAACCTG GTGTCCCAGT CTGGGTTAGA TTTACAACAA 
    GTTTCTGGTT ATACTGTGGT TGCAGAAAAA ATTAACTTCT ACCGTTTTAT CTCTCATTTT 
    AGAATGGTTC ATAGAGGCTC CTTTTTTGCG CAATTGAAAA CTACCACAGT CAGAAAGTTG 
    CTACCTGAGT CGTGGGGATT CCTGTGCCCT GTTCATACAC CAGATGGTTC TCCTTGTGGT 
    TTATTAAACC ATTTTGCGCA CAAGTGTCGT ATATCAACTC AGCAATCTGA CGTCTCAAGA 
    ATTCCTTCTA TATTATACTC ACTTGGTGTT GCTCCGGCAT CTCACACCTT TGCTGCTGGT 
    CCTTCTCTGT GTTGTGTTCA AATTGATGGT AAAATTATTG GCTGGGTCTC TCATGAACAA 
    GGTAAGATCA TTGCGGATAC ATTGAGATAT TGGAAAGTTG AAGGCAAGAC GCCTGGGTTA 
    CCGATTGATC TAGAAATTGG ATATGTTCCT CCCTCAACTC GTGGTCAATA TCCAGGTCTC 
    TACCTATTTG GTGGTCATTC TAGGATGCTG CGTCCTGTTC GTTACCTGCC TTTGGATAAA 
    GAAGACATTG TTGGACCTTT TGAGCAAGTG TACATGAACA TTGCTGTCAC TCCTCAAGAA 
    ATTCAAAATA ACGTTCATAC ACACGTAGAA TTTACGCCCA CAAATATTCT TTCTATTTTG 
    GCTAATTTGA CTCCATTTTC TGATTTCAAC CAGTCTCCAA GAAACATGTA TCAATGTCAG 
    ATGGGTAAAC AAACAATGGG TACTCCGGGT GTAGCTTTGT GCCATCGTTC CGACAATAAG 
    CTGTATAGGT TGCAAACAGG TCAAACGCCT ATCGTAAAAG CTAATTTATA TGATGATTAT 
    GGCATGGATA ACTTTCCAAA CGGGTTTAAT GCTGTTGTTG CGGTCATCTC GTATACTGGT 
    TATGATATGG ATGATGCAAT GATTATAAAC AAATCAGCAG ATGAAAGAGG TTTCGGTTAT 
    GGTACTATGT ATAAGACTGA GAAAGTGGAT TTAGCACTTA ACAGAAACCG TGGTGACCCA 
    ATTACACAGC ATTTTGGTTT CGGGAATGAC GAATGGCCAA AGGAGTGGCT TGAGAAATTA 
    GACGAGGATG GTTTGCCATA CATTGGCACT TATGTTGAAG AGGGTGATCC AATCTGCGCA 
    TACTTTGACG ATACTTTAAA TAAGACCAAA ATCAAGACAT ATCACTCCTC TGAACCAGCA 
    TATATCGAAG AGGTAAATTT AATCGGTGAT GAATCCAATA AGTTCCAAGA ACTACAAACT 
    GTTAGCATAA AATATCGTAT TAGAAGAACG CCTCAAATTG GTGATAAATT TTCTTCTAGA 
    CACGGTCAAA AAGGTGTTTG TTCAAGAAAA TGGCCAACCA TTGATATGCC TTTTAGCGAA 
    ACAGGTATCC AACCAGACAT CATCATTAAC CCTCATGCTT TCCCTTCGCG TATGACCATT 
    GGTATGTTCG TCGAATCTTT AGCAGGTAAA GCGGGGGCAT TGCATGGTAT TGCACAGGAC 
    TCTACACCTT GGATCTTTAA CGAGGACGAT ACCCCAGCCG ATTACTTTGG TGAGCAACTA 
    GCGAAGGCGG GTTACAACTA CCATGGTAAT GAGCCAATGT ACTCAGGTGC TACTGGTGAA 
    GAATTAAGGG CAGATATTTA TGTTGGTGTT GTCTACTACC AAAGATTACG TCATATGGTC 
    AATGACAAGT TCCAAGTTCG TTCCACTGGT CCTGTGAATA GTTTGACTAT GCAGCCTGTC 
    AAAGGTAGAA AGAGACATGG TGGTATTCGT GTCGGTGAAA TGGAAAGAGA TGCTTTGATT 
    GGGCATGGTA CATCTTTTCT ATTACAAGAT CGTCTGCTAA ATTCATCAGA CTACACACAG 
    GCATCCGTAT GTCGCGAGTG TGGTTCTATT TTGACCACGC AACAGAGTGT GCCAAGAATT 
    GGTTCGATTT CCACCGTTTG TTGCCGTCGT TGTTCCATGA GATTCGAAGA TGCAAAGAAG 
    CTATTGACTA AATCAGAAGA TGGAGAGAAA ATTTTTATTG ACGACTCTCA AATTTGGGAA 
    GATGGACAAG GTAACAAATT TGTTGGTGGT AATGAAACAA CTACGGTGGC CATACCATTT 
    GTGTTGAAGT ACTTGGACTC TGAGCTATCC GCAATGGGTA TAAGATTGCG TTATAATGTA 
    GAGCCCAAAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_015335.1
    CDS1..3612
    Translation

    Target ORF information:

    RefSeq Version NM_001184107.1
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C DNA-directed RNA polymerase I core subunit RPA135 (RPA135), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001184107.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGCAAAG TGATTAAGCC GCCTGGCCAA GCCAGAACTG CTGATTTCCG TACCTTAGAA 
    CGTGAATCTA GGTTCATCAA TCCACCTAAG GATAAATCAG CATTTCCACT ATTACAGGAA 
    GCTGTTCAAC CTCATATTGG GTCTTTTAAC GCATTAACAG AAGGTCCGGA TGGAGGTTTG 
    TTAAATCTTG GTGTTAAAGA CATCGGTGAA AAAGTTATAT TTGATGGAAA ACCACTCAAT 
    TCTGAAGACG AAATATCCAA CAGCGGATAC CTCGGGAATA AGCTTTCTGT TAGTGTGGAA 
    CAAGTGTCTA TTGCGAAGCC AATGTCTAAC GATGGTGTTT CTTCTGCAGT AGAGAGAAAA 
    GTTTACCCAA GTGAGTCAAG ACAAAGGCTT ACTTCTTACA GAGGTAAATT ACTTTTAAAG 
    TTGAAGTGGT CCGTGAACAA TGGCGAGGAG AACTTATTTG AAGTAAGAGA TTGTGGTGGT 
    CTACCAGTTA TGCTACAGAG TAACAGATGT CACTTGAACA AGATGTCACC ATATGAACTG 
    GTACAACACA AGGAAGAATC CGATGAAATA GGTGGTTATT TCATTGTTAA CGGTATAGAA 
    AAGCTTATTA GAATGCTGAT TGTGCAACGT AGAAATCATC CTATGGCTAT CATCAGACCT 
    TCCTTCGCTA ACAGGGGTGC ATCCTATTCT CATTACGGTA TTCAAATTAG ATCTGTCAGG 
    CCGGATCAAA CTTCCCAAAC TAATGTTTTG CATTACTTAA ATGACGGTCA AGTTACATTT 
    AGATTTTCTT GGAGAAAAAA TGAATATTTA GTTCCTGTTG TTATGATTTT AAAAGCCTTG 
    TGTCATACCA GCGATAGGGA AATTTTTGAT GGTATTATTG GTAATGACGT TAAGGATTCC 
    TTTTTGACCG ATCGTTTAGA ATTACTGTTA CGTGGTTTTA AGAAAAGATA TCCTCATTTA 
    CAAAATCGCA CACAAGTCTT GCAATATTTA GGAGACAAAT TTCGTGTTGT CTTTCAAGCT 
    TCCCCTGATC AATCTGATTT AGAAGTCGGA CAAGAAGTCC TTGATCGCAT CGTTCTCGTG 
    CATTTGGGTA AGGATGGCAG CCAGGATAAA TTCAGAATGT TATTGTTCAT GATCAGAAAG 
    TTGTATTCCT TGGTTGCAGG CGAATGTTCT CCAGATAACC CAGATGCTAC GCAACATCAA 
    GAAGTTCTTT TGGGTGGTTT CTTATATGGT ATGATCCTAA AAGAAAAGAT TGATGAGTAC 
    TTACAGAATA TTATTGCTCA AGTCAGGATG GATATTAACC GTGGTATGGC CATTAATTTC 
    AAAGACAAAA GGTACATGTC AAGAGTTTTA ATGAGGGTTA ATGAAAACAT TGGTTCCAAA 
    ATGCAATACT TTTTATCAAC TGGTAACCTG GTGTCCCAGT CTGGGTTAGA TTTACAACAA 
    GTTTCTGGTT ATACTGTGGT TGCAGAAAAA ATTAACTTCT ACCGTTTTAT CTCTCATTTT 
    AGAATGGTTC ATAGAGGCTC CTTTTTTGCG CAATTGAAAA CTACCACAGT CAGAAAGTTG 
    CTACCTGAGT CGTGGGGATT CCTGTGCCCT GTTCATACAC CAGATGGTTC TCCTTGTGGT 
    TTATTAAACC ATTTTGCGCA CAAGTGTCGT ATATCAACTC AGCAATCTGA CGTCTCAAGA 
    ATTCCTTCTA TATTATACTC ACTTGGTGTT GCTCCGGCAT CTCACACCTT TGCTGCTGGT 
    CCTTCTCTGT GTTGTGTTCA AATTGATGGT AAAATTATTG GCTGGGTCTC TCATGAACAA 
    GGTAAGATCA TTGCGGATAC ATTGAGATAT TGGAAAGTTG AAGGCAAGAC GCCTGGGTTA 
    CCGATTGATC TAGAAATTGG ATATGTTCCT CCCTCAACTC GTGGTCAATA TCCAGGTCTC 
    TACCTATTTG GTGGTCATTC TAGGATGCTG CGTCCTGTTC GTTACCTGCC TTTGGATAAA 
    GAAGACATTG TTGGACCTTT TGAGCAAGTG TACATGAACA TTGCTGTCAC TCCTCAAGAA 
    ATTCAAAATA ACGTTCATAC ACACGTAGAA TTTACGCCCA CAAATATTCT TTCTATTTTG 
    GCTAATTTGA CTCCATTTTC TGATTTCAAC CAGTCTCCAA GAAACATGTA TCAATGTCAG 
    ATGGGTAAAC AAACAATGGG TACTCCGGGT GTAGCTTTGT GCCATCGTTC CGACAATAAG 
    CTGTATAGGT TGCAAACAGG TCAAACGCCT ATCGTAAAAG CTAATTTATA TGATGATTAT 
    GGCATGGATA ACTTTCCAAA CGGGTTTAAT GCTGTTGTTG CGGTCATCTC GTATACTGGT 
    TATGATATGG ATGATGCAAT GATTATAAAC AAATCAGCAG ATGAAAGAGG TTTCGGTTAT 
    GGTACTATGT ATAAGACTGA GAAAGTGGAT TTAGCACTTA ACAGAAACCG TGGTGACCCA 
    ATTACACAGC ATTTTGGTTT CGGGAATGAC GAATGGCCAA AGGAGTGGCT TGAGAAATTA 
    GACGAGGATG GTTTGCCATA CATTGGCACT TATGTTGAAG AGGGTGATCC AATCTGCGCA 
    TACTTTGACG ATACTTTAAA TAAGACCAAA ATCAAGACAT ATCACTCCTC TGAACCAGCA 
    TATATCGAAG AGGTAAATTT AATCGGTGAT GAATCCAATA AGTTCCAAGA ACTACAAACT 
    GTTAGCATAA AATATCGTAT TAGAAGAACG CCTCAAATTG GTGATAAATT TTCTTCTAGA 
    CACGGTCAAA AAGGTGTTTG TTCAAGAAAA TGGCCAACCA TTGATATGCC TTTTAGCGAA 
    ACAGGTATCC AACCAGACAT CATCATTAAC CCTCATGCTT TCCCTTCGCG TATGACCATT 
    GGTATGTTCG TCGAATCTTT AGCAGGTAAA GCGGGGGCAT TGCATGGTAT TGCACAGGAC 
    TCTACACCTT GGATCTTTAA CGAGGACGAT ACCCCAGCCG ATTACTTTGG TGAGCAACTA 
    GCGAAGGCGG GTTACAACTA CCATGGTAAT GAGCCAATGT ACTCAGGTGC TACTGGTGAA 
    GAATTAAGGG CAGATATTTA TGTTGGTGTT GTCTACTACC AAAGATTACG TCATATGGTC 
    AATGACAAGT TCCAAGTTCG TTCCACTGGT CCTGTGAATA GTTTGACTAT GCAGCCTGTC 
    AAAGGTAGAA AGAGACATGG TGGTATTCGT GTCGGTGAAA TGGAAAGAGA TGCTTTGATT 
    GGGCATGGTA CATCTTTTCT ATTACAAGAT CGTCTGCTAA ATTCATCAGA CTACACACAG 
    GCATCCGTAT GTCGCGAGTG TGGTTCTATT TTGACCACGC AACAGAGTGT GCCAAGAATT 
    GGTTCGATTT CCACCGTTTG TTGCCGTCGT TGTTCCATGA GATTCGAAGA TGCAAAGAAG 
    CTATTGACTA AATCAGAAGA TGGAGAGAAA ATTTTTATTG ACGACTCTCA AATTTGGGAA 
    GATGGACAAG GTAACAAATT TGTTGGTGGT AATGAAACAA CTACGGTGGC CATACCATTT 
    GTGTTGAAGT ACTTGGACTC TGAGCTATCC GCAATGGGTA TAAGATTGCG TTATAATGTA 
    GAGCCCAAAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
    Nature387(6632 Suppl)103-5(1997 May)
    Bussey H,Storms RK,Ahmed A,Albermann K,Allen E,Ansorge W,Araujo R,Aparicio A,Barrell B,Badcock K,Benes V,Botstein D,Bowman S,Br?ckner M,Carpenter J,Cherry JM,Chung E,Churcher C,Coster F,Davis K,Davis RW,Dietrich FS,Delius H,DiPaolo T,Hani J


    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


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