MET12 cDNA ORF clone, Saccharomyces cerevisiae S288C

The following MET12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MET12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi04941 NM_001183837.1
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Saccharomyces cerevisiae S288C methylenetetrahydrofolate reductase (NAD(P)H) MET12 (MET12), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSi04941
    Clone ID Related Accession (Same CDS sequence) NM_001183837.1
    Accession Version NM_001183837.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1974bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product methylenetetrahydrofolate reductase (NAD(P)H) MET12
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001148). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTCCATCA GAGATTTATA TCATGCGAGG GCTTCCCCTT TTATATCGTT AGAATTCTTC 
    CCTCCAAAGA CTGAATTAGG GACGAGAAAT TTGATGGAAC GTATGCATCG TATGACTGCT
    TTAGATCCAC TGTTTATCAC GGTTACTTGG GGAGCAGGTG GTACTACTGC GGAAAAGACT
    CTGACATTAG CTTCCTTGGC ACAGCAGACA CTAAATATAC CAGTTTGTAT GCATTTGACC
    TGTACAAACA CAGAAAAAGC CATCATTGAT GATGCGCTGG ATAGATGTTA TAATGCAGGA
    ATCAGGAATA TTTTGGCTCT TCGAGGTGAC CCACCTATTG GGGAAGATTG GCTAGATTCT
    CAATCGAACG AATCACCTTT TAAATATGCG GTTGATTTAG TTCGTTATAT CAAGCAAAGC
    TACGGAGACA AGTTCTGCGT CGGTGTTGCA GCATATCCAG AAGGTCATTG TGAAGGTGAA
    GCAGAAGGTC ACGAGCAAGA CCCATTGAAG GATTTGGTAT ATTTAAAAGA AAAAGTTGAA
    GCTGGGGCCG ATTTTGTGAT AACACAACTG TTTTACGACG TTGAAAAATT CTTAACTTTT
    GAAATGCTAT TTCGGGAACG GATTTCGCAA GATTTGCCCC TTTTCCCTGG GTTGATGCCT
    ATTAACTCCT ATCTGCTTTT CCACAGAGCA GCAAAGTTAT CACATGCATC TATTCCACCT
    GCAATACTGA GTAGGTTCCC CCCAGAAATC CAATCGGATG ATAATGCCGT GAAGTCCATT
    GGTGTGGACA TTCTTATCGA ATTGATTCAG GAAATATATC AAAGAACATC TGGTAGAATT
    AAAGGGTTTC ATTTCTATAC ATTAAATTTG GAAAAGGCTA TTGCTCAAAT TGTCTCGCAA
    TCTCCCGTCT TATCCCATAT CGTAAATGAA TCTAGCGAAG AAGAGGGAGA AGATGAAACC
    AGTGGCGAAA TAGGAAGCAT AGAAAATGTG CCAATAGAAG ATGCTGACGG GGATATTGTG
    TTGGATGATT CGAATGAAGA AACTGTAGCA AACCGGAAAA GGAGAAGGCA TTCAAGTCTT
    GATTCGGCCA AGTTGATTTT CAATAGGGCT ATCGTCACTG AAAAAGGCTT ACGCTATAAT
    AATGAGAATG GTTCCATGCC ATCTAAAAAG GCTTTAATAT CTATTTCTAA AGGTCACGGA
    ACTTTGGGTC GTGATGCCAC TTGGGATGAG TTCCCCAATG GTAGATTTGG TGACTCCAGG
    TCTCCCGCAT ATGGTGAAAT AGACGGTTAT GGGCCATCCA TCAAGGTAAG CAAAAGCAAA
    GCGCTTGAAC TATGGGGTAT ACCAAAGACA ATTGGCGATT TAAAAGATAT TTTTATAAAA
    TATTTGGAAG GTTCAACGGA TGCGATTCCC TGGTCTGACC TGGGTTTATC AGCTGAAACG
    GCGTTAATAC AAGAAGAACT AATCCAACTA AACTATCGCG GATATTTGAC TTTGGCATCG
    CAACCTGCCA CAAATGCTAC ATTGAGTAGT GATAAAATAT TCGGCTGGGG GCCCGCGAAA
    GGGAGGTTGT ATCAGAAGGC ATTCGTAGAA ATGTTTATTC ATAGACAGCA ATGGGAAACA
    ACTTTGAAAC CCAAGCTAGA CCATTACGGG CGTCGGAAAT TCAGTTATTA CGCGGGCGAT
    TCATCTGGTT CATTTGAAAC GAACCTAGAC CCGCACAGCT CTAGCGTTGT AACATGGGGT
    GTTTTTCCCA ACAGTCCGGT CAAACAGACT ACAATCATTG AAGAAGAGTC ATTCAAGGCA
    TGGAGAGATG AAGCGTTTAG TATCTGGTCC GAGTGGGCCA AGTTATTTCC AAGGAACACC
    CCAGCCAATA TTCTTCTAAG ACTAGTGCAT AAAGACTACT GTCTTGTCTC AATTGTTCAT
    CACGACTTCA AAGAAACCGA CGAGCTATGG GAAATGTTAC TCGACCAAGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_015302.1
    CDS1..1974
    Translation

    Target ORF information:

    RefSeq Version NM_001183837.1
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C methylenetetrahydrofolate reductase (NAD(P)H) MET12 (MET12), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001183837.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTCCATCA GAGATTTATA TCATGCGAGG GCTTCCCCTT TTATATCGTT AGAATTCTTC 
    CCTCCAAAGA CTGAATTAGG GACGAGAAAT TTGATGGAAC GTATGCATCG TATGACTGCT
    TTAGATCCAC TGTTTATCAC GGTTACTTGG GGAGCAGGTG GTACTACTGC GGAAAAGACT
    CTGACATTAG CTTCCTTGGC ACAGCAGACA CTAAATATAC CAGTTTGTAT GCATTTGACC
    TGTACAAACA CAGAAAAAGC CATCATTGAT GATGCGCTGG ATAGATGTTA TAATGCAGGA
    ATCAGGAATA TTTTGGCTCT TCGAGGTGAC CCACCTATTG GGGAAGATTG GCTAGATTCT
    CAATCGAACG AATCACCTTT TAAATATGCG GTTGATTTAG TTCGTTATAT CAAGCAAAGC
    TACGGAGACA AGTTCTGCGT CGGTGTTGCA GCATATCCAG AAGGTCATTG TGAAGGTGAA
    GCAGAAGGTC ACGAGCAAGA CCCATTGAAG GATTTGGTAT ATTTAAAAGA AAAAGTTGAA
    GCTGGGGCCG ATTTTGTGAT AACACAACTG TTTTACGACG TTGAAAAATT CTTAACTTTT
    GAAATGCTAT TTCGGGAACG GATTTCGCAA GATTTGCCCC TTTTCCCTGG GTTGATGCCT
    ATTAACTCCT ATCTGCTTTT CCACAGAGCA GCAAAGTTAT CACATGCATC TATTCCACCT
    GCAATACTGA GTAGGTTCCC CCCAGAAATC CAATCGGATG ATAATGCCGT GAAGTCCATT
    GGTGTGGACA TTCTTATCGA ATTGATTCAG GAAATATATC AAAGAACATC TGGTAGAATT
    AAAGGGTTTC ATTTCTATAC ATTAAATTTG GAAAAGGCTA TTGCTCAAAT TGTCTCGCAA
    TCTCCCGTCT TATCCCATAT CGTAAATGAA TCTAGCGAAG AAGAGGGAGA AGATGAAACC
    AGTGGCGAAA TAGGAAGCAT AGAAAATGTG CCAATAGAAG ATGCTGACGG GGATATTGTG
    TTGGATGATT CGAATGAAGA AACTGTAGCA AACCGGAAAA GGAGAAGGCA TTCAAGTCTT
    GATTCGGCCA AGTTGATTTT CAATAGGGCT ATCGTCACTG AAAAAGGCTT ACGCTATAAT
    AATGAGAATG GTTCCATGCC ATCTAAAAAG GCTTTAATAT CTATTTCTAA AGGTCACGGA
    ACTTTGGGTC GTGATGCCAC TTGGGATGAG TTCCCCAATG GTAGATTTGG TGACTCCAGG
    TCTCCCGCAT ATGGTGAAAT AGACGGTTAT GGGCCATCCA TCAAGGTAAG CAAAAGCAAA
    GCGCTTGAAC TATGGGGTAT ACCAAAGACA ATTGGCGATT TAAAAGATAT TTTTATAAAA
    TATTTGGAAG GTTCAACGGA TGCGATTCCC TGGTCTGACC TGGGTTTATC AGCTGAAACG
    GCGTTAATAC AAGAAGAACT AATCCAACTA AACTATCGCG GATATTTGAC TTTGGCATCG
    CAACCTGCCA CAAATGCTAC ATTGAGTAGT GATAAAATAT TCGGCTGGGG GCCCGCGAAA
    GGGAGGTTGT ATCAGAAGGC ATTCGTAGAA ATGTTTATTC ATAGACAGCA ATGGGAAACA
    ACTTTGAAAC CCAAGCTAGA CCATTACGGG CGTCGGAAAT TCAGTTATTA CGCGGGCGAT
    TCATCTGGTT CATTTGAAAC GAACCTAGAC CCGCACAGCT CTAGCGTTGT AACATGGGGT
    GTTTTTCCCA ACAGTCCGGT CAAACAGACT ACAATCATTG AAGAAGAGTC ATTCAAGGCA
    TGGAGAGATG AAGCGTTTAG TATCTGGTCC GAGTGGGCCA AGTTATTTCC AAGGAACACC
    CCAGCCAATA TTCTTCTAAG ACTAGTGCAT AAAGACTACT GTCTTGTCTC AATTGTTCAT
    CACGACTTCA AAGAAACCGA CGAGCTATGG GAAATGTTAC TCGACCAAGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
    Nature387(6632 Suppl)103-5(1997 May)
    Bussey H,Storms RK,Ahmed A,Albermann K,Allen E,Ansorge W,Araujo R,Aparicio A,Barrell B,Badcock K,Benes V,Botstein D,Bowman S,Br?ckner M,Carpenter J,Cherry JM,Chung E,Churcher C,Coster F,Davis K,Davis RW,Dietrich FS,Delius H,DiPaolo T,Hani J


    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG