ORF » Species Summary » Saccharomyces cerevisiae S288C » ISM1 cDNA ORF clone

ISM1 cDNA ORF clone, Saccharomyces cerevisiae S288C

The following ISM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ISM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi04926 NM_001183854.1
Latest version!		 Saccharomyces cerevisiae S288C isoleucine--tRNA ligase ISM1 (ISM1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    ISM1 ( NM_001183854.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_015285.1 Saccharomyces cerevisiae S288C isoleucine--tRNA ligase ISM1 (ISM1), partial mRNA.
    Price & Availability↑
    CloneID OSi04926
    Clone ID Related Accession (Same CDS sequence) NM_001183854.1
    Accession Version NM_001183854.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3009bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product isoleucine--tRNA ligase ISM1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001148). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAAGCGCT CCAGGCTTGT GCCTCAACAT ATCTTTAGCA TTATTAGCAA GCGATATCTC 
    GCCAAACATG CCTACCAAAA AACATTAAAC TTGCCCAAGA CCAAATTCCC TAATAGATCA 
    AACTTAGAGA TTACATTGAG GGAATTAATA CCAAAATCTT CGCAGTTAGT TTACAAGGAG 
    CAGCTACGTG ATTTCTTCGA AGAATTTTCA AAGCTTAATA CGACTGATGA AAAATTAGAG 
    TTCATTAAAG AAAAGTTGTT CATCTTACAT GATGGACCGC CCTATGCCAA CGGCGAGCTC 
    CATCTCGGTC ATGCTCTAAA TAAAATTCTG AAAGATATCA TAAACAGATA TCAACTTTCA 
    CAGGGGAAGT ACATTTTTTA TAAGCCTGGC TGGGATTGTC ATGGACTACC CATCGAGATT 
    AAAGCTTTGA AAGATTTAAG CGCGCAACAG ATCGAGTCAA TATCGCCTTT AAAAATCAGG 
    TCAATGGCTT TAAAACATGC ACAAAAGGCA ATCAAGAGGC AAAGGGAAAC TTTCCAACAT 
    TTTGCCATCT TAACGGACTG GGAAACACCA TACCTAACAA TGGATAAAGA CTATGAAATT 
    AATCAGCTAA ATATTTTTAA AGAAATGTAC GAAAGAGGAC TGATTAAGAG ACAGAACAAA 
    CCGGTTTACT GGGGTACAGA AACTAGAACA GCGTTAGCAG AGGGCGAATT AGAATATAAT 
    GAAAACCATA AATCCATCGC TGCTTATGTT AAATTTCCTC TTGAAAAGAA ATCCCAGATG 
    GATCTGTGCA AAAAACTAGG AATAACGAAT AACTTGCCTA TATACTGTTT GATTTGGACC 
    AGTACTCCAT GGACTCTTCT CTCTAATCGA GCAATCTGTT TCAATCAGGA TTTTTCCTAC 
    TCCCTATTAC GCTTAAATAG TGAACTTATC CTTGTAGAGA CCGGCTCCAT TGATAAATTG 
    GGCCTAACAA CTAACTCATT TGAGACTATC AAACAGTTTC AAGGAACTCA TCTAAATGGG 
    CTATATTATC AAAACCTCTT AGTAGACGAT AAGGTTGGCA GGCCCTTATT ACATGGTGCA 
    CACGTTACCA GCGGTACAGG GACTGGATTG GTTCATACAG CACCAGGGCA TGGACAGGAT 
    GACTATCTCA TTGGTATTCA AAATGGTCTT GAAATTTATT CTCCCGTGGA TCACCAGGGC 
    CGCTACCAAT TAAATGAACT TCCTCAGTCC GTCAGATCCA TCGTGAGAGA TGAAGGAGAC 
    TTAACAAAAG GTAGGCAAGT ATTGGATGCC GAAACCGCGA AAATCATTCT ATGTAAGTTA 
    AGCGACCTTA ATTTACTTTA CAAATCTCAC GAATACACGC ATTCTTATCC GTACGACTGG 
    AGATCCAAGA AACCTGTCAT AATAAGAGCT ACTCCACAGT GGTTTGCAGA TTTACACGAT 
    GTAAAAAACT TGGCTCTGGA AAGCATCAGC CGCGTCAAGT TTTGTCCTAA AAGAGGCTAT 
    TCAAGACTTT CATCCTTTAT GAAAAGCAGA AATGAATGGT GCATATCTAG GCAACGCTCA 
    TGGGGTATCC CTATATTAAG CTTTTACAAG AAGAGCGAAC CGGATTCTGT TTTGATGAAT 
    TCAGAAATTT TGGCACATGC CATAGAAAAA ATCAAACAAA AAGGAATTAA TGCATGGTTT 
    AACGATAAAG ATAATGACAT GAAAGAATGG TTGCCAGAAA AATATCACGA CGTAGCGCAT 
    GAATACTGTC GTTCTCAAGA TACTATGGAT GTGTGGTTTG ATAGTGGGTC GTCATGGAGT 
    GTGATCAAAG ACTTTTATGA AAAAAGCTTA AAGTTGTCAA AACTCCCATC CCCCCTGTAC 
    CAAGTTTGTC TAGAAGGATC TGATCAACAT AGAGGATGGT TTCAAAGTTC ACTGCTAACA 
    AAAGTAGCAT CAAGTAATGT CCCTGTTGCA CCATATGAAG AAGTGATTAC TCATGGTTTT 
    ACCCTAGATG AGAATGGTCT GAAAATGTCA AAATCTGTGG GAAATACAAT TTCTCCCGAA 
    GCAATAATTC GAGGCGATGA AAACTTAGGC TTACCAGCTT TGGGTGTTGA TGGCTTGAGG 
    TATCTGATAG CACAATCGAA TTTCACAACT GATATAGTTG CTGGCCCGAC TGTGATGAAA 
    CATGTAGGAG AAGCTCTAAA AAAGGTTAGA CTAACTTTTC GCTATTTATT GAGTAATTTA 
    CAGAAGTCCC AAGATTTCAA CCTTTTGCCG ATTGAACAAT TACGCCGTGT TGATCAATAT 
    ACCTTGTATA AGATAAACGA ACTGCTGGAA ACGACGAGAG AACACTACCA AAAGTACAAC 
    TTTTCCAAGG TTCTCATTAC TCTACAATAT CATTTAAATA ACGAGCTATC GGCGTTTTAT 
    TTTGATATCT CAAAGGATAT TTTATATTCC AACCAAATAT CGTCATTGGC AAGAAGGCAG 
    GTCCAAACAA CGCTTGTCCA TATACTAAAT GCATATAGGG CAATTCTTGC ACCAATATTA 
    CCCGTTATGG TCCAAGAAGT ATGGAAGTAT ATACCAGAAG GATGGTTACA AGGACAAGAA 
    CATATAGACA TTAATCCGAT GCGTGGAAAA TGGCCGTTTT TGGACTCAAA TACGGAAATC 
    GTCACCTCCT TTGAAAACTT TGAACTAAAA ATTCTAAAAC AGTTCCAGGA GGAATTCAAG 
    AGGTTAAGTC TAGAAGAAGG TGTCACCAAA ACAACACATA GTCACGTTAC CATTTTCACC 
    AAGCATCACC TCCCATTCAG TTCTGACGAG CTTTGCGACA TATTGCAATC ATCTGCTGTT 
    GATATATTGC AAATGGACGA TAATAATAAC AGTCTACCAA CTATTGAATT GGGAAGCGGA 
    ATAAACGTTC AAATATTGGT TGAACGAAGC AAAAGGCACA ACTGTCCCAG ATGTTGGAAG 
    GCCAACTCAG CCGAAGAGGA TAAATTGTGT GACAGATGTA AAGAAGCTGT GGATCATCTC 
    ATGTCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_015285.1
    CDS1..3009
    Translation

    Target ORF information:

    RefSeq Version NM_001183854.1
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C isoleucine--tRNA ligase ISM1 (ISM1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001183854.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAAGCGCT CCAGGCTTGT GCCTCAACAT ATCTTTAGCA TTATTAGCAA GCGATATCTC 
    GCCAAACATG CCTACCAAAA AACATTAAAC TTGCCCAAGA CCAAATTCCC TAATAGATCA 
    AACTTAGAGA TTACATTGAG GGAATTAATA CCAAAATCTT CGCAGTTAGT TTACAAGGAG 
    CAGCTACGTG ATTTCTTCGA AGAATTTTCA AAGCTTAATA CGACTGATGA AAAATTAGAG 
    TTCATTAAAG AAAAGTTGTT CATCTTACAT GATGGACCGC CCTATGCCAA CGGCGAGCTC 
    CATCTCGGTC ATGCTCTAAA TAAAATTCTG AAAGATATCA TAAACAGATA TCAACTTTCA 
    CAGGGGAAGT ACATTTTTTA TAAGCCTGGC TGGGATTGTC ATGGACTACC CATCGAGATT 
    AAAGCTTTGA AAGATTTAAG CGCGCAACAG ATCGAGTCAA TATCGCCTTT AAAAATCAGG 
    TCAATGGCTT TAAAACATGC ACAAAAGGCA ATCAAGAGGC AAAGGGAAAC TTTCCAACAT 
    TTTGCCATCT TAACGGACTG GGAAACACCA TACCTAACAA TGGATAAAGA CTATGAAATT 
    AATCAGCTAA ATATTTTTAA AGAAATGTAC GAAAGAGGAC TGATTAAGAG ACAGAACAAA 
    CCGGTTTACT GGGGTACAGA AACTAGAACA GCGTTAGCAG AGGGCGAATT AGAATATAAT 
    GAAAACCATA AATCCATCGC TGCTTATGTT AAATTTCCTC TTGAAAAGAA ATCCCAGATG 
    GATCTGTGCA AAAAACTAGG AATAACGAAT AACTTGCCTA TATACTGTTT GATTTGGACC 
    AGTACTCCAT GGACTCTTCT CTCTAATCGA GCAATCTGTT TCAATCAGGA TTTTTCCTAC 
    TCCCTATTAC GCTTAAATAG TGAACTTATC CTTGTAGAGA CCGGCTCCAT TGATAAATTG 
    GGCCTAACAA CTAACTCATT TGAGACTATC AAACAGTTTC AAGGAACTCA TCTAAATGGG 
    CTATATTATC AAAACCTCTT AGTAGACGAT AAGGTTGGCA GGCCCTTATT ACATGGTGCA 
    CACGTTACCA GCGGTACAGG GACTGGATTG GTTCATACAG CACCAGGGCA TGGACAGGAT 
    GACTATCTCA TTGGTATTCA AAATGGTCTT GAAATTTATT CTCCCGTGGA TCACCAGGGC 
    CGCTACCAAT TAAATGAACT TCCTCAGTCC GTCAGATCCA TCGTGAGAGA TGAAGGAGAC 
    TTAACAAAAG GTAGGCAAGT ATTGGATGCC GAAACCGCGA AAATCATTCT ATGTAAGTTA 
    AGCGACCTTA ATTTACTTTA CAAATCTCAC GAATACACGC ATTCTTATCC GTACGACTGG 
    AGATCCAAGA AACCTGTCAT AATAAGAGCT ACTCCACAGT GGTTTGCAGA TTTACACGAT 
    GTAAAAAACT TGGCTCTGGA AAGCATCAGC CGCGTCAAGT TTTGTCCTAA AAGAGGCTAT 
    TCAAGACTTT CATCCTTTAT GAAAAGCAGA AATGAATGGT GCATATCTAG GCAACGCTCA 
    TGGGGTATCC CTATATTAAG CTTTTACAAG AAGAGCGAAC CGGATTCTGT TTTGATGAAT 
    TCAGAAATTT TGGCACATGC CATAGAAAAA ATCAAACAAA AAGGAATTAA TGCATGGTTT 
    AACGATAAAG ATAATGACAT GAAAGAATGG TTGCCAGAAA AATATCACGA CGTAGCGCAT 
    GAATACTGTC GTTCTCAAGA TACTATGGAT GTGTGGTTTG ATAGTGGGTC GTCATGGAGT 
    GTGATCAAAG ACTTTTATGA AAAAAGCTTA AAGTTGTCAA AACTCCCATC CCCCCTGTAC 
    CAAGTTTGTC TAGAAGGATC TGATCAACAT AGAGGATGGT TTCAAAGTTC ACTGCTAACA 
    AAAGTAGCAT CAAGTAATGT CCCTGTTGCA CCATATGAAG AAGTGATTAC TCATGGTTTT 
    ACCCTAGATG AGAATGGTCT GAAAATGTCA AAATCTGTGG GAAATACAAT TTCTCCCGAA 
    GCAATAATTC GAGGCGATGA AAACTTAGGC TTACCAGCTT TGGGTGTTGA TGGCTTGAGG 
    TATCTGATAG CACAATCGAA TTTCACAACT GATATAGTTG CTGGCCCGAC TGTGATGAAA 
    CATGTAGGAG AAGCTCTAAA AAAGGTTAGA CTAACTTTTC GCTATTTATT GAGTAATTTA 
    CAGAAGTCCC AAGATTTCAA CCTTTTGCCG ATTGAACAAT TACGCCGTGT TGATCAATAT 
    ACCTTGTATA AGATAAACGA ACTGCTGGAA ACGACGAGAG AACACTACCA AAAGTACAAC 
    TTTTCCAAGG TTCTCATTAC TCTACAATAT CATTTAAATA ACGAGCTATC GGCGTTTTAT 
    TTTGATATCT CAAAGGATAT TTTATATTCC AACCAAATAT CGTCATTGGC AAGAAGGCAG 
    GTCCAAACAA CGCTTGTCCA TATACTAAAT GCATATAGGG CAATTCTTGC ACCAATATTA 
    CCCGTTATGG TCCAAGAAGT ATGGAAGTAT ATACCAGAAG GATGGTTACA AGGACAAGAA 
    CATATAGACA TTAATCCGAT GCGTGGAAAA TGGCCGTTTT TGGACTCAAA TACGGAAATC 
    GTCACCTCCT TTGAAAACTT TGAACTAAAA ATTCTAAAAC AGTTCCAGGA GGAATTCAAG 
    AGGTTAAGTC TAGAAGAAGG TGTCACCAAA ACAACACATA GTCACGTTAC CATTTTCACC 
    AAGCATCACC TCCCATTCAG TTCTGACGAG CTTTGCGACA TATTGCAATC ATCTGCTGTT 
    GATATATTGC AAATGGACGA TAATAATAAC AGTCTACCAA CTATTGAATT GGGAAGCGGA 
    ATAAACGTTC AAATATTGGT TGAACGAAGC AAAAGGCACA ACTGTCCCAG ATGTTGGAAG 
    GCCAACTCAG CCGAAGAGGA TAAATTGTGT GACAGATGTA AAGAAGCTGT GGATCATCTC 
    ATGTCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
    Nature387(6632 Suppl)103-5(1997 May)
    Bussey H,Storms RK,Ahmed A,Albermann K,Allen E,Ansorge W,Araujo R,Aparicio A,Barrell B,Badcock K,Benes V,Botstein D,Bowman S,Br?ckner M,Carpenter J,Cherry JM,Chung E,Churcher C,Coster F,Davis K,Davis RW,Dietrich FS,Delius H,DiPaolo T,Hani J


    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


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