MOT1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	MOT1
Entrez Gene ID	856023
Full Name	DNA-binding ATPase
Synonyms	BUR3,END10,LPF4
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001183896.1	NP_015243.1	DNA-binding ATPase

Pathways from BioSystems

Homologies

Homo sapiens (human)	BTAF1	NP_003963.1
Danio rerio (zebrafish)	btaf1	XP_005169589.1
Saccharomyces cerevisiae (baker's yeast)	MOT1	NP_015243.1
Gallus gallus (chicken)	BTAF1	XP_421689.3
Eremothecium gossypii	AGOS_AEL256C	NP_984605.2
Magnaporthe oryzae (rice blast fungus)	MGG_04989	XP_003712489.1
Pan troglodytes (chimpanzee)	BTAF1	XP_003312799.1
Canis lupus familiaris (dog)	BTAF1	XP_003640032.1
Bos taurus (cattle)	BTAF1	XP_003587915.2
Arabidopsis thaliana (thale cress)	RGD3	NP_190996.3
Rattus norvegicus (Norway rat)	Btaf1	NP_001178846.1
Drosophila melanogaster (fruit fly)	Hel89B	NP_732097.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001820	XP_321235.5
Schizosaccharomyces pombe (fission yeast)	mot1	NP_596080.2
	BTAF1	XP_002805795.1
Mus musculus (house mouse)	Btaf1	NP_001074175.1
Kluyveromyces lactis	KLLA0E23717g	XP_455023.1
Neurospora crassa	NCU07556	XP_958601.1
Xenopus tropicalis (tropical clawed frog)	btaf1	XP_002939139.2

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
Bussey H, Storms RK, Ahmed A, Albermann K, Allen E, Ansorge W, Araujo R, Aparicio A, Barrell B, Badcock K, Benes V, Botstein D, Bowman S, Br?ckner M, Carpenter J, Cherry JM, Chung E, Churcher C, Coster F, Davis K, Davis RW, Dietrich FS, Delius H, DiPaolo T, Hani J
Nature387(6632 Suppl)103-5(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mot1, Ino80 chromatin remodeling complex (Ino80C), and NC2 co-localize on chromatin and coordinately suppress pervasive transcription in S. cerevisiae.
Title: Mot1, Ino80C, and NC2 Function Coordinately to Regulate Pervasive Transcription in Yeast and Mammals.

these results suggest that TBP-DNA affinity as well as other aspects of promoter sequence influence Mot1 function in vivo.
Title: Molecular Mechanism of Mot1, a TATA-binding Protein (TBP)-DNA Dissociating Enzyme.

These results suggest that the large scale overlap in Mot1 and Spt16 function arises from a combination of both their unique and shared functions in transcription complex assembly and chromatin structure regulation.
Title: The Modifier of Transcription 1 (Mot1) ATPase and Spt16 Histone Chaperone Co-regulate Transcription through Preinitiation Complex Assembly and Nucleosome Organization.

The results stress the importance of the cooperation of Mot1p/NC2 with remodelers in maintaining a repressive environment for intragenic transcription.
Title: Suppression of intragenic transcription requires the MOT1 and NC2 regulators of TATA-binding protein.

The action of Mot1 is required to clear TBP from intrinsically preferred (TATA-containing) binding sites, ensuring sufficient soluble TBP to bind intrinsically disfavored (TATA-less) sites.
Title: Mot1 redistributes TBP from TATA-containing to TATA-less promoters.

The following MOT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MOT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04889	NM_001183896.1 Latest version!	Saccharomyces cerevisiae S288C DNA-binding ATPase (MOT1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MOT1 ( NM_001183896.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_015243.1 Saccharomyces cerevisiae S288C DNA-binding ATPase (MOT1), partial mRNA.

Price & Availability↑

CloneID	OSi04889
Clone ID Related Accession (Same CDS sequence)	NM_001183896.1
Accession Version	NM_001183896.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5604bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	DNA-binding ATPase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001148). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGACGTCAC GAGTTTCGAG GCTGGACCGG CAGGTAATAT TAATAGAGAC CGGTTCCACC 
CAAGTAGTCC GGAACATGGC TGCCGATCAA ATGGGAGACT TGGCCAAACA GCACCCCGAA 
GACATTCTTT CGTTGCTGTC CAGAGTTTAC CCCTTTTTAC TAGTGAAGAA ATGGGAAACG 
CGTGTTACTG CAGCAAGAGC TGTGGGTGGC ATAGTTGCCC ATGCTCCTTC TTGGGATCCG 
AATGAATCTG ACTTGGTAGG TGGTACAAAT GAAGGTTCTC CATTGGATAA TGCTCAGGTG 
AAGTTGGAAC ATGAAATGAA AATAAAATTG GAAGAGGCTA CTCAGAACAA TCAGTTGAAT 
CTCTTACAGG AGGACCATCA CCTCAGTTCT CTGTCTGATT GGAAATTGAA CGAAATTTTA 
AAGTCAGGTA AAGTGCTCCT GGCGTCGAGT ATGAATGATT ACAATGTTCT TGGTAAGGCT 
GACGATAATA TAAGAAAACA AGCTAAAACT GATGATATAA AACAGGAAAC ATCTATGCTA 
AATGCGTCTG ATAAGGCGAA TGAAAATAAA AGTAATGCTA ACAAGAAGTC AGCAAGGATG 
CTTGCTATGG CAAGGCGAAA AAAGAAAATG AGTGCTAAGA ATACTCCCAA ACATCCTGTA 
GATATAACGG AAAGTTCAGT TTCCAAGACT TTATTAAATG GAAAAAATAT GACCAATTCT 
GCTGCATCGC TAGCCACTTC TCCCACCAGT AACCAGCTAA ATCCCAAACT GGAGATTACT 
GAACAAGCCG ATGAAAGTAA ACTAATGATA GAATCCACAG TAAGGCCATT GCTAGAACAA 
CACGAAATTG TTGCTGGCCT AGTTTGGCAA TTCCAAGGCA TTTATGAACT ACTTTTAGAT 
AATCTAATGA GCGAAAATTG GGAAATAAGG CATGGAGCTG CGTTGGGTCT TCGAGAATTG 
GTAAAAAAAC ATGCTTATGG AGTCAGCAGG GTTAAGGGGA ATACCCGAGA GGAGAACAAC 
CTGAGGAATT CTAGAAGCTT AGAAGACTTG GCGTCCCGTC TACTTACAGT ATTTGCATTG 
GATAGATTTG GTGATTATGT TTATGATACA GTTGTGGCCC CTGTACGAGA GTCAGTTGCG 
CAAACTCTAG CTGCGTTATT AATTCACCTA GACAGTACCT TATCAATTAA AATATTTAAT 
TGTTTAGAAC AACTGGTTCT ACAAGATCCA CTACAAACAG GTCTACCGAA CAAAATATGG 
GAAGCTACCC ATGGAGGGCT TTTAGGGATT CGTTACTTCG TCAGCATAAA GACAAATTTC 
CTATTTGCCC ATGGCCTTTT AGAAAACGTT GTCCGTATAG TGCTATATGG TTTAAACCAA 
AGTGATGATG ACGTTCAAAG CGTAGCAGCC TCAATTTTAA CCCCAATAAC CAGCGAATTT 
GTCAAATTAA ACAACTCTAC CATAGAAATT TTGGTGACCA CGATATGGTC ATTACTAGCT 
CGTTTAGATG ATGATATATC TTCAAGCGTA GGTTCTATAA TGGATCTATT GGCAAAGTTG 
TGCGACCACC AAGAAGTACT AGATATTTTA AAGAACAAGG CTTTAGAGCA TCCTTCAGAG 
TGGTCATTCA AGTCACTGGT ACCGAAATTG TATCCCTTTT TACGCCATTC GATTTCTTCT 
GTGAGGAGGG CTGTTTTGAA TCTACTGATT GCATTCTTAT CAATAAAGGA TGATTCTACT 
AAAAACTGGT TAAACGGCAA GGTATTCCGT CTAGTTTTTC AGAATATTTT ACTGGAGCAA 
AACCCGGAAC TATTGCAACT GTCTTTTGAT GTTTATGTTG CGTTATTGGA GCATTATAAA 
GTTAAGCATA CCGAAAAAAC TTTAGATCAT GTTTTCAGTA AACATTTGCA GCCAATACTA 
CATCTTCTAA ATACGCCAGT AGGGGAAAAG GGAAAGAATT ATGCCATGGA ATCACAATAT 
ATTCTAAAGC CTTCCCAGCA CTATCAATTA CATCCAGAAA AAAAACGTTC TATATCTGAA 
ACCACAACAG ATTCTGACAT TCCAATACCA AAAAATAATG AACATATCAA TATCGATGCA 
CCTATGATTG CTGGAGATAT TACTTTGCTA GGATTGGACG TAATATTGAA CACTAGAATA 
ATGGGAGCAA AGGCATTTGC TTTGACATTG TCAATGTTTC AAGATTCCAC GCTTCAATCC 
TTCTTCACAA ACGTTCTGGT TCGTTGTTTA GAGCTGCCAT TCTCAACTCC ACGTATGTTG 
GCAGGCATTA TTGTTTCACA GTTTTGCTCC TCGTGGTTAC AAAAGCATCC AGAAGGGGAA 
AAATTACCTT CTTTTGTATC AGAGATTTTC AGTCCTGTGA TGAATAAACA GTTACTGAAT 
CGCGATGAAT TTCCTGTATT TAGGGAGCTA GTACCTAGCT TAAAAGCTCT GAGGACTCAA 
TGTCAAAGCC TACTGGCTAC CTTTGTGGAT GTAGGTATGT TACCTCAGTA CAAACTACCC 
AACGTAGCAA TTGTGGTTCA AGGCGAAACA GAAGCCGGTC CACATGCGTT TGGTGTTGAA 
ACAGCAGAAA AAGTGTACGG CGAATACTAT GATAAAATGT TCAAATCCAT GAACAACTCT 
TACAAACTAT TGGCCAAGAA GCCATTAGAA GATTCTAAGC ATCGCGTGTT GATGGCAATC 
AATTCAGCCA AAGAATCCGC AAAACTTCGT ACAGGCAGCA TTCTCGCTAA TTACGCATCA 
TCCATCTTGT TATTTGATGG TCTACCTCTT AAATTAAACC CCATTATCAG GTCGCTAATG 
GACAGTGTTA AAGAAGAAAG GAATGAAAAG TTACAAACGA TGGCCGGAGA ATCGGTCGTA 
CATTTGATTC AGCAACTGTT AGAAAATAAT AAAGTTAATG TGTCTGGTAA GATAGTCAAA 
AATCTCTGCG GATTTTTATG CGTTGATACT TCAGAAGTTC CTGATTTCTC CGTAAATGCA 
GAATATAAAG AAAAAATATT GACGTTAATA AAAGAATCTA ATTCGATTGC CGCACAAGAT 
GACATTAATC TCGCAAAGAT GTCGGAAGAG GCTCAACTAA AAAGGAAGGG TGGGTTGATT 
ACGTTAAAAA TACTATTTGA AGTTCTTGGG CCTTCTATAT TACAGAAACT ACCTCAGCTT 
AGATCGATTT TATTTGATTC ATTATCTGAT CATGAAAATG AGGAAGCAAG TAAAGTTGAC 
AACGAGCAAG GTCAAAAAAT AGTCGACTCA TTTGGTGTAC TAAGAGCTCT ATTCCCATTT 
ATGTCTGATT CGCTACGTTC TAGTGAGGTT TTTACCCGTT TCCCTGTTTT ATTAACGTTC 
TTAAGATCAA ACTTATCTGT TTTTCGTTAT TCAGCTGCAA GAACTTTTGC TGATCTGGCT 
AAAATTTCCT CCGTGGAGGT AATGGCTTAC ACCATACGTG AGATTTTGCC TTTAATGAAT 
AGCGCTGGTT CTCTATCCGA TCGTCAAGGG TCCACGGAGC TTATTTATCA TTTATCACTT 
TCTATGGAAA CCGATGTTCT ACCTTACGTC ATTTTCTTAA TAGTTCCACT TTTGGGTCGC 
ATGAGTGATT CGAACGAAGA TGTGAGGAAT TTAGCTACAA CAACATTTGC TTCTATCATC 
AAATTAGTTC CTTTAGAAGC TGGTATCGCC GATCCAAAAG GACTCCCAGA AGAACTAGTG 
GCAAGCAGAG AGAGGGAGCG TGATTTTATC CAACAAATGA TGGATCCTTC TAAAGCTAAG 
CCATTTAAAT TACCCATTGC CATCAAGGCG ACTTTAAGAA AATATCAGCA AGATGGTGTT 
AACTGGTTGG CATTCCTGAA CAAATATCAT CTACATGGAA TATTGTGTGA CGATATGGGT 
TTGGGTAAAA CATTACAAAC CATCTGCATT ATCGCAAGCG ATCAATATTT GAGGAAGGAA 
GATTATGAAA AAACGCGTTC TGTTGAAAGT AGAGCCTTAC CATCTCTCAT AATATGCCCC 
CCATCTTTAA CTGGCCATTG GGAAAACGAG TTTGACCAGT ATGCTCCCTT TTTAAAGGTT 
GTTGTGTATG CCGGTGGCCC AACTGTCAGA TTAACTCTGA GACCACAGTT AAGTGATGCT 
GATATTATTG TAACATCATA TGACGTAGCT AGAAATGATC TCGCAGTCCT AAACAAAACT 
GAATATAATT ATTGTGTTTT GGATGAAGGA CATATAATTA AAAACTCTCA ATCAAAACTT 
GCTAAGGCTG TTAAAGAAAT TACTGCAAAC CATAGATTAA TTTTAACCGG AACTCCCATC 
CAAAACAACG TTTTAGAATT GTGGTCTCTG TTTGACTTCT TAATGCCGGG ATTTTTGGGA 
ACGGAAAAGA TGTTCCAGGA AAGGTTCGCA AAGCCAATCG CTGCGTCAAG AAATAGTAAA 
ACATCATCTA AGGAGCAGGA AGCAGGTGTA CTGGCTTTGG AGGCACTACA TAAGCAAGTT 
CTACCTTTCA TGCTGAGAAG ATTGAAGGAA GACGTTCTAT CTGATTTACC ACCTAAAATT 
ATTCAGGATT ACTACTGTGA ATTAGGTGAT TTACAAAAAC AACTATACAT GGATTTTACT 
AAAAAACAAA AAAATGTAGT TGAGAAAGAT ATTGAAAATT CTGAGATTGC TGATGGCAAG 
CAACATATTT TCCAAGCTTT ACAATACATG AGAAAATTGT GTAATCATCC AGCTTTGGTC 
CTTTCACCAA ATCACCCGCA ATTAGCGCAA GTACAAGACT ATTTAAAGCA AACTGGTCTA 
GATTTACACG ATATTATCAA CGCTCCAAAA CTGAGCGCAT TGAGAACATT ACTCTTTGAA 
TGTGGTATAG GTGAAGAAGA TATCGACAAA AAAGCAAGCC AAGATCAGAA TTTTCCTATT 
CAAAATGTCA TATCACAACA CAGAGCCCTG ATTTTCTGCC AACTAAAAGA TATGCTAGAC 
ATGGTTGAAA ATGACTTGTT TAAAAAGTAT ATGCCCTCCG TAACCTATAT GAGGCTAGAT 
GGAAGCATTG ACCCAAGAGA CAGACAAAAA GTTGTTCGGA AATTTAACGA AGATCCCTCT 
ATTGATTGCC TACTGTTGAC CACCAAGGTC GGAGGGCTGG GTCTGAATTT AACTGGTGCA 
GACACCGTCA TTTTTGTAGA GCATGACTGG AATCCAATGA ATGATCTACA GGCAATGGAC 
AGAGCACATA GAATTGGTCA GAAAAAGGTT GTTAATGTTT ACAGAATTAT TACGAAAGGT 
ACGCTTGAAG AAAAAATCAT GGGTTTGCAG AAATTCAAAA TGAATATAGC TTCTACAGTT 
GTTAATCAGC AAAACAGTGG ATTAGCATCC ATGGATACAC ATCAGCTGCT TGATCTCTTC 
GACCCCGATA ACGTTACCTC ACAGGACAAT GAGGAAAAGA ATAATGGCGA TTCCCAAGCA 
GCCAAGGGCA TGGAAGATAT TGCCAACGAG ACTGGTTTAA CTGGCAAAGC CAAAGAGGCC 
CTCGGTGAAT TGAAAGAGCT ATGGGACCCA TCTCAATACG AGGAGGAGTA TAATTTAGAC 
ACCTTCATCA AAACTTTACG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_015243.1
CDS	1..5604
Translation	MTSRVSRLDRQVILIETGSTQVVRNMAADQMGDLAKQHPEDILSLLSRVYPFLLVKKWETRVTAARAVGGIVAHAPSWDPNESDLVGGTNEGSPLDNAQVKLEHEMKIKLEEATQNNQLNLLQEDHHLSSLSDWKLNEILKSGKVLLASSMNDYNVLGKADDNIRKQAKTDDIKQETSMLNASDKANENKSNANKKSARMLAMARRKKKMSAKNTPKHPVDITESSVSKTLLNGKNMTNSAASLATSPTSNQLNPKLEITEQADESKLMIESTVRPLLEQHEIVAGLVWQFQGIYELLLDNLMSENWEIRHGAALGLRELVKKHAYGVSRVKGNTREENNLRNSRSLEDLASRLLTVFALDRFGDYVYDTVVAPVRESVAQTLAALLIHLDSTLSIKIFNCLEQLVLQDPLQTGLPNKIWEATHGGLLGIRYFVSIKTNFLFAHGLLENVVRIVLYGLNQSDDDVQSVAASILTPITSEFVKLNNSTIEILVTTIWSLLARLDDDISSSVGSIMDLLAKLCDHQEVLDILKNKALEHPSEWSFKSLVPKLYPFLRHSISSVRRAVLNLLIAFLSIKDDSTKNWLNGKVFRLVFQNILLEQNPELLQLSFDVYVALLEHYKVKHTEKTLDHVFSKHLQPILHLLNTPVGEKGKNYAMESQYILKPSQHYQLHPEKKRSISETTTDSDIPIPKNNEHINIDAPMIAGDITLLGLDVILNTRIMGAKAFALTLSMFQDSTLQSFFTNVLVRCLELPFSTPRMLAGIIVSQFCSSWLQKHPEGEKLPSFVSEIFSPVMNKQLLNRDEFPVFRELVPSLKALRTQCQSLLATFVDVGMLPQYKLPNVAIVVQGETEAGPHAFGVETAEKVYGEYYDKMFKSMNNSYKLLAKKPLEDSKHRVLMAINSAKESAKLRTGSILANYASSILLFDGLPLKLNPIIRSLMDSVKEERNEKLQTMAGESVVHLIQQLLENNKVNVSGKIVKNLCGFLCVDTSEVPDFSVNAEYKEKILTLIKESNSIAAQDDINLAKMSEEAQLKRKGGLITLKILFEVLGPSILQKLPQLRSILFDSLSDHENEEASKVDNEQGQKIVDSFGVLRALFPFMSDSLRSSEVFTRFPVLLTFLRSNLSVFRYSAARTFADLAKISSVEVMAYTIREILPLMNSAGSLSDRQGSTELIYHLSLSMETDVLPYVIFLIVPLLGRMSDSNEDVRNLATTTFASIIKLVPLEAGIADPKGLPEELVASRERERDFIQQMMDPSKAKPFKLPIAIKATLRKYQQDGVNWLAFLNKYHLHGILCDDMGLGKTLQTICIIASDQYLRKEDYEKTRSVESRALPSLIICPPSLTGHWENEFDQYAPFLKVVVYAGGPTVRLTLRPQLSDADIIVTSYDVARNDLAVLNKTEYNYCVLDEGHIIKNSQSKLAKAVKEITANHRLILTGTPIQNNVLELWSLFDFLMPGFLGTEKMFQERFAKPIAASRNSKTSSKEQEAGVLALEALHKQVLPFMLRRLKEDVLSDLPPKIIQDYYCELGDLQKQLYMDFTKKQKNVVEKDIENSEIADGKQHIFQALQYMRKLCNHPALVLSPNHPQLAQVQDYLKQTGLDLHDIINAPKLSALRTLLFECGIGEEDIDKKASQDQNFPIQNVISQHRALIFCQLKDMLDMVENDLFKKYMPSVTYMRLDGSIDPRDRQKVVRKFNEDPSIDCLLLTTKVGGLGLNLTGADTVIFVEHDWNPMNDLQAMDRAHRIGQKKVVNVYRIITKGTLEEKIMGLQKFKMNIASTVVNQQNSGLASMDTHQLLDLFDPDNVTSQDNEEKNNGDSQAAKGMEDIANETGLTGKAKEALGELKELWDPSQYEEEYNLDTFIKTLR

Target ORF information:

RefSeq Version	NM_001183896.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C DNA-binding ATPase (MOT1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001183896.1

ORF Insert Sequence:

ATGACGTCAC GAGTTTCGAG GCTGGACCGG CAGGTAATAT TAATAGAGAC CGGTTCCACC 
CAAGTAGTCC GGAACATGGC TGCCGATCAA ATGGGAGACT TGGCCAAACA GCACCCCGAA 
GACATTCTTT CGTTGCTGTC CAGAGTTTAC CCCTTTTTAC TAGTGAAGAA ATGGGAAACG 
CGTGTTACTG CAGCAAGAGC TGTGGGTGGC ATAGTTGCCC ATGCTCCTTC TTGGGATCCG 
AATGAATCTG ACTTGGTAGG TGGTACAAAT GAAGGTTCTC CATTGGATAA TGCTCAGGTG 
AAGTTGGAAC ATGAAATGAA AATAAAATTG GAAGAGGCTA CTCAGAACAA TCAGTTGAAT 
CTCTTACAGG AGGACCATCA CCTCAGTTCT CTGTCTGATT GGAAATTGAA CGAAATTTTA 
AAGTCAGGTA AAGTGCTCCT GGCGTCGAGT ATGAATGATT ACAATGTTCT TGGTAAGGCT 
GACGATAATA TAAGAAAACA AGCTAAAACT GATGATATAA AACAGGAAAC ATCTATGCTA 
AATGCGTCTG ATAAGGCGAA TGAAAATAAA AGTAATGCTA ACAAGAAGTC AGCAAGGATG 
CTTGCTATGG CAAGGCGAAA AAAGAAAATG AGTGCTAAGA ATACTCCCAA ACATCCTGTA 
GATATAACGG AAAGTTCAGT TTCCAAGACT TTATTAAATG GAAAAAATAT GACCAATTCT 
GCTGCATCGC TAGCCACTTC TCCCACCAGT AACCAGCTAA ATCCCAAACT GGAGATTACT 
GAACAAGCCG ATGAAAGTAA ACTAATGATA GAATCCACAG TAAGGCCATT GCTAGAACAA 
CACGAAATTG TTGCTGGCCT AGTTTGGCAA TTCCAAGGCA TTTATGAACT ACTTTTAGAT 
AATCTAATGA GCGAAAATTG GGAAATAAGG CATGGAGCTG CGTTGGGTCT TCGAGAATTG 
GTAAAAAAAC ATGCTTATGG AGTCAGCAGG GTTAAGGGGA ATACCCGAGA GGAGAACAAC 
CTGAGGAATT CTAGAAGCTT AGAAGACTTG GCGTCCCGTC TACTTACAGT ATTTGCATTG 
GATAGATTTG GTGATTATGT TTATGATACA GTTGTGGCCC CTGTACGAGA GTCAGTTGCG 
CAAACTCTAG CTGCGTTATT AATTCACCTA GACAGTACCT TATCAATTAA AATATTTAAT 
TGTTTAGAAC AACTGGTTCT ACAAGATCCA CTACAAACAG GTCTACCGAA CAAAATATGG 
GAAGCTACCC ATGGAGGGCT TTTAGGGATT CGTTACTTCG TCAGCATAAA GACAAATTTC 
CTATTTGCCC ATGGCCTTTT AGAAAACGTT GTCCGTATAG TGCTATATGG TTTAAACCAA 
AGTGATGATG ACGTTCAAAG CGTAGCAGCC TCAATTTTAA CCCCAATAAC CAGCGAATTT 
GTCAAATTAA ACAACTCTAC CATAGAAATT TTGGTGACCA CGATATGGTC ATTACTAGCT 
CGTTTAGATG ATGATATATC TTCAAGCGTA GGTTCTATAA TGGATCTATT GGCAAAGTTG 
TGCGACCACC AAGAAGTACT AGATATTTTA AAGAACAAGG CTTTAGAGCA TCCTTCAGAG 
TGGTCATTCA AGTCACTGGT ACCGAAATTG TATCCCTTTT TACGCCATTC GATTTCTTCT 
GTGAGGAGGG CTGTTTTGAA TCTACTGATT GCATTCTTAT CAATAAAGGA TGATTCTACT 
AAAAACTGGT TAAACGGCAA GGTATTCCGT CTAGTTTTTC AGAATATTTT ACTGGAGCAA 
AACCCGGAAC TATTGCAACT GTCTTTTGAT GTTTATGTTG CGTTATTGGA GCATTATAAA 
GTTAAGCATA CCGAAAAAAC TTTAGATCAT GTTTTCAGTA AACATTTGCA GCCAATACTA 
CATCTTCTAA ATACGCCAGT AGGGGAAAAG GGAAAGAATT ATGCCATGGA ATCACAATAT 
ATTCTAAAGC CTTCCCAGCA CTATCAATTA CATCCAGAAA AAAAACGTTC TATATCTGAA 
ACCACAACAG ATTCTGACAT TCCAATACCA AAAAATAATG AACATATCAA TATCGATGCA 
CCTATGATTG CTGGAGATAT TACTTTGCTA GGATTGGACG TAATATTGAA CACTAGAATA 
ATGGGAGCAA AGGCATTTGC TTTGACATTG TCAATGTTTC AAGATTCCAC GCTTCAATCC 
TTCTTCACAA ACGTTCTGGT TCGTTGTTTA GAGCTGCCAT TCTCAACTCC ACGTATGTTG 
GCAGGCATTA TTGTTTCACA GTTTTGCTCC TCGTGGTTAC AAAAGCATCC AGAAGGGGAA 
AAATTACCTT CTTTTGTATC AGAGATTTTC AGTCCTGTGA TGAATAAACA GTTACTGAAT 
CGCGATGAAT TTCCTGTATT TAGGGAGCTA GTACCTAGCT TAAAAGCTCT GAGGACTCAA 
TGTCAAAGCC TACTGGCTAC CTTTGTGGAT GTAGGTATGT TACCTCAGTA CAAACTACCC 
AACGTAGCAA TTGTGGTTCA AGGCGAAACA GAAGCCGGTC CACATGCGTT TGGTGTTGAA 
ACAGCAGAAA AAGTGTACGG CGAATACTAT GATAAAATGT TCAAATCCAT GAACAACTCT 
TACAAACTAT TGGCCAAGAA GCCATTAGAA GATTCTAAGC ATCGCGTGTT GATGGCAATC 
AATTCAGCCA AAGAATCCGC AAAACTTCGT ACAGGCAGCA TTCTCGCTAA TTACGCATCA 
TCCATCTTGT TATTTGATGG TCTACCTCTT AAATTAAACC CCATTATCAG GTCGCTAATG 
GACAGTGTTA AAGAAGAAAG GAATGAAAAG TTACAAACGA TGGCCGGAGA ATCGGTCGTA 
CATTTGATTC AGCAACTGTT AGAAAATAAT AAAGTTAATG TGTCTGGTAA GATAGTCAAA 
AATCTCTGCG GATTTTTATG CGTTGATACT TCAGAAGTTC CTGATTTCTC CGTAAATGCA 
GAATATAAAG AAAAAATATT GACGTTAATA AAAGAATCTA ATTCGATTGC CGCACAAGAT 
GACATTAATC TCGCAAAGAT GTCGGAAGAG GCTCAACTAA AAAGGAAGGG TGGGTTGATT 
ACGTTAAAAA TACTATTTGA AGTTCTTGGG CCTTCTATAT TACAGAAACT ACCTCAGCTT 
AGATCGATTT TATTTGATTC ATTATCTGAT CATGAAAATG AGGAAGCAAG TAAAGTTGAC 
AACGAGCAAG GTCAAAAAAT AGTCGACTCA TTTGGTGTAC TAAGAGCTCT ATTCCCATTT 
ATGTCTGATT CGCTACGTTC TAGTGAGGTT TTTACCCGTT TCCCTGTTTT ATTAACGTTC 
TTAAGATCAA ACTTATCTGT TTTTCGTTAT TCAGCTGCAA GAACTTTTGC TGATCTGGCT 
AAAATTTCCT CCGTGGAGGT AATGGCTTAC ACCATACGTG AGATTTTGCC TTTAATGAAT 
AGCGCTGGTT CTCTATCCGA TCGTCAAGGG TCCACGGAGC TTATTTATCA TTTATCACTT 
TCTATGGAAA CCGATGTTCT ACCTTACGTC ATTTTCTTAA TAGTTCCACT TTTGGGTCGC 
ATGAGTGATT CGAACGAAGA TGTGAGGAAT TTAGCTACAA CAACATTTGC TTCTATCATC 
AAATTAGTTC CTTTAGAAGC TGGTATCGCC GATCCAAAAG GACTCCCAGA AGAACTAGTG 
GCAAGCAGAG AGAGGGAGCG TGATTTTATC CAACAAATGA TGGATCCTTC TAAAGCTAAG 
CCATTTAAAT TACCCATTGC CATCAAGGCG ACTTTAAGAA AATATCAGCA AGATGGTGTT 
AACTGGTTGG CATTCCTGAA CAAATATCAT CTACATGGAA TATTGTGTGA CGATATGGGT 
TTGGGTAAAA CATTACAAAC CATCTGCATT ATCGCAAGCG ATCAATATTT GAGGAAGGAA 
GATTATGAAA AAACGCGTTC TGTTGAAAGT AGAGCCTTAC CATCTCTCAT AATATGCCCC 
CCATCTTTAA CTGGCCATTG GGAAAACGAG TTTGACCAGT ATGCTCCCTT TTTAAAGGTT 
GTTGTGTATG CCGGTGGCCC AACTGTCAGA TTAACTCTGA GACCACAGTT AAGTGATGCT 
GATATTATTG TAACATCATA TGACGTAGCT AGAAATGATC TCGCAGTCCT AAACAAAACT 
GAATATAATT ATTGTGTTTT GGATGAAGGA CATATAATTA AAAACTCTCA ATCAAAACTT 
GCTAAGGCTG TTAAAGAAAT TACTGCAAAC CATAGATTAA TTTTAACCGG AACTCCCATC 
CAAAACAACG TTTTAGAATT GTGGTCTCTG TTTGACTTCT TAATGCCGGG ATTTTTGGGA 
ACGGAAAAGA TGTTCCAGGA AAGGTTCGCA AAGCCAATCG CTGCGTCAAG AAATAGTAAA 
ACATCATCTA AGGAGCAGGA AGCAGGTGTA CTGGCTTTGG AGGCACTACA TAAGCAAGTT 
CTACCTTTCA TGCTGAGAAG ATTGAAGGAA GACGTTCTAT CTGATTTACC ACCTAAAATT 
ATTCAGGATT ACTACTGTGA ATTAGGTGAT TTACAAAAAC AACTATACAT GGATTTTACT 
AAAAAACAAA AAAATGTAGT TGAGAAAGAT ATTGAAAATT CTGAGATTGC TGATGGCAAG 
CAACATATTT TCCAAGCTTT ACAATACATG AGAAAATTGT GTAATCATCC AGCTTTGGTC 
CTTTCACCAA ATCACCCGCA ATTAGCGCAA GTACAAGACT ATTTAAAGCA AACTGGTCTA 
GATTTACACG ATATTATCAA CGCTCCAAAA CTGAGCGCAT TGAGAACATT ACTCTTTGAA 
TGTGGTATAG GTGAAGAAGA TATCGACAAA AAAGCAAGCC AAGATCAGAA TTTTCCTATT 
CAAAATGTCA TATCACAACA CAGAGCCCTG ATTTTCTGCC AACTAAAAGA TATGCTAGAC 
ATGGTTGAAA ATGACTTGTT TAAAAAGTAT ATGCCCTCCG TAACCTATAT GAGGCTAGAT 
GGAAGCATTG ACCCAAGAGA CAGACAAAAA GTTGTTCGGA AATTTAACGA AGATCCCTCT 
ATTGATTGCC TACTGTTGAC CACCAAGGTC GGAGGGCTGG GTCTGAATTT AACTGGTGCA 
GACACCGTCA TTTTTGTAGA GCATGACTGG AATCCAATGA ATGATCTACA GGCAATGGAC 
AGAGCACATA GAATTGGTCA GAAAAAGGTT GTTAATGTTT ACAGAATTAT TACGAAAGGT 
ACGCTTGAAG AAAAAATCAT GGGTTTGCAG AAATTCAAAA TGAATATAGC TTCTACAGTT 
GTTAATCAGC AAAACAGTGG ATTAGCATCC ATGGATACAC ATCAGCTGCT TGATCTCTTC 
GACCCCGATA ACGTTACCTC ACAGGACAAT GAGGAAAAGA ATAATGGCGA TTCCCAAGCA 
GCCAAGGGCA TGGAAGATAT TGCCAACGAG ACTGGTTTAA CTGGCAAAGC CAAAGAGGCC 
CTCGGTGAAT TGAAAGAGCT ATGGGACCCA TCTCAATACG AGGAGGAGTA TAATTTAGAC 
ACCTTCATCA AAACTTTACG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
Nature387(6632 Suppl)103-5(1997 May)
Bussey H,Storms RK,Ahmed A,Albermann K,Allen E,Ansorge W,Araujo R,Aparicio A,Barrell B,Badcock K,Benes V,Botstein D,Bowman S,Br?ckner M,Carpenter J,Cherry JM,Chung E,Churcher C,Coster F,Davis K,Davis RW,Dietrich FS,Delius H,DiPaolo T,Hani J

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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MOT1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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