SEC16 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	SEC16
Entrez Gene ID	856020
Full Name	Sec16p
Synonyms	LPF1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001183899.1	NP_015240.1	Sec16p

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
Bussey H, Storms RK, Ahmed A, Albermann K, Allen E, Ansorge W, Araujo R, Aparicio A, Barrell B, Badcock K, Benes V, Botstein D, Bowman S, Br?ckner M, Carpenter J, Cherry JM, Chung E, Churcher C, Coster F, Davis K, Davis RW, Dietrich FS, Delius H, DiPaolo T, Hani J
Nature387(6632 Suppl)103-5(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the present study sheds light on both the structural and the regulatory functions of Sec16. Our data explain at least in part the mechanism for how Sec16 functions in relation to the COPII coat proteins in the ER.
Title: Insights into structural and regulatory roles of Sec16 in COPII vesicle formation at ER exit sites.

association of Sec24p with Sec16p creates a regulatory complex that retards the GTPase activity of the COPII coat to prevent premature vesicle scission
Title: Sec24p and Sec16p cooperate to regulate the GTP cycle of the COPII coat.

The following SEC16 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SEC16 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04886	NM_001183899.1 Latest version!	Saccharomyces cerevisiae S288C Sec16p (SEC16), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SEC16 ( NM_001183899.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_015240.1 Saccharomyces cerevisiae S288C Sec16p (SEC16), partial mRNA.

Price & Availability↑

CloneID	OSi04886
Clone ID Related Accession (Same CDS sequence)	NM_001183899.1
Accession Version	NM_001183899.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6588bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Sec16p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001148). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGACACCTG AAGCCAAGAA AAGGAAAAAC CAAAAGAAGA AGTTGAAGCA AAAGCAAAAA 
AAAGCTGCTG AGAAAGCTGC TAGCCACAGT GAAGAACCAC TTGAATTACC AGAAAGTACG 
ATTAACAGCA GCTTCAACGA CGACTCGGTG AACCGTACAG AATCTGACAT AGCTTCAAAA 
TCTGATGTTC CTCCGGTCTC ATCATCTACC AATATCTCTC CGGCTAATGA AACACAACTA 
GAAATACCTG ATACTCAAGA ATTGCATCAT AAACTGCTCA ACGACTCTGA TCAACATGAT 
ATTACCGCGG ACTCAAATGA TTTGCCAGAC AACTCAATCG TTGAACATGA CTCTGTTATT 
ACCCAAACAA AACCAGCCAT GTCTCAAGAA TACGAAGAGA CTGCCGCTCA CTTATCTTCG 
AGAAATCCAT CGCTCGATGT AGTCGCGGGA GAACTTCACA ATAATAATGA ACATACCCAG 
AAAATTGCCG TATCCGCTGT GGAAGAGGAT TCTTTCAATG AAGAAGAGGG TGAAAATCAC 
GACAGCATAA TAATTTCATC ATTAAACGAT GCTACCCCTT CTCAATATAA TCATTTTCTC 
CCATCCGATG GCAATCTTCT TTCTCCAGAA TTATCTTCTG GTGATACGCC AACTCACAAT 
GTTCCTCTAG GCACAAAAGA CAATGAAATA AATGACGATG AGTATTGTAA TGATAAGGAA 
ATTAGTTTGA ACGCAAATAA TGTGCTTCCT GATGAACTTT CAAAGGAAGA AGATGAAAGA 
TTAAAACTAG AAACGCATGT ATCAACCGAA GAAAAGAAAC AGGATATCGC TGATCAGGAA 
ACTGCAGAAA ACTTATTTAC GTCTAGTACA GAACCATCTG AGAATAAAAT AAGAAATTCT 
GGTGATGATA CCTCCATGTT GTTTCAAGAT GACGAAAGTG ATCAGAAGGT TCCATGGGAG 
GAAGATGTGA AGAAAGATTT TCATAATGAG AACACAAATA ATACTCAAGA ATCGGCACCG 
AACACAGATG ATCGTGATAA GGGTTATGAA GGAAACGAAG CTTTGAAAAA GTCCGAAAGT 
TGTACAGCCG CGGACGAGAG GTCGTACTCT GAAGAAACTT CAGAAGATAT CTTTCACGGA 
CACGACAAAC AGGTAGTTGA AGGCCAAAAT GATTTCACTG GGAAAAATAT TGAGAATGAA 
AGCCAGAAAT TAATGGGGGA AGGGAATCAT AAGTTACCGT TGTCTGCCGA AGCTGACATT 
ATAGAACCTG GTAAGGATAT TCAAGATCAA GCCGAGGATT TGTTTACGCA GAGCAGCGGA 
GACTTGGGAG AAGTTTTGCC ATGGGAATCT ACTGATAAAA ACGCTGATGT AACGAGCAAA 
TCCCAAGAGA AACATGAAGA TTTATTTGCT GCTTCTGGAA ACGATGAGAA ACTTCCTTGG 
GAAGTTTCTG ACGGTGAAGT ATCATCGGGA AAGACGGAAA ACAGCATGCA GACTAGTACT 
GAGAAAATAG CTGAGCAAAA GTTTTCGTTT TTGGAAAACG ACGACGACCT TTTGGACGAC 
GACGACAGCT TTTTGGCTTC TTCTGAGGAA GAAGACACAG TACCTAATAC CGATAATACA 
ACGAATTTAA CCTCAAAACC AGTTGAAGAA AAAAAGGCTT CAAGATATAA ACCTATTATC 
GAGGAGGAAG CAGGAATGCG TCAAGAGCAA GTTCATTTTA CCAATACTAC TGGCATTGTA 
ACACCGCAGC AGTTCCACGG TTTGACTAAA ACTGGACTAG GCACCCCCAA CCAACAAGTC 
AGTGTACCAA ATATAGTTAG TCCTAAGCCT CCTGTGGTAA AAGACAATCG TTCAAATTTT 
AAGATAAATG AGGAGAAAAA GAAGTCTGAT GCTTACGATT TTCCACTGGA AATTATTTCA 
GAAAGTTCCA AGAAGGGTCA CGCAAAGCCG GTTGCCGTTC CTACTCAAAG GTTTGGCTCA 
GGGAATTCTT TTAGTTCTTT GGACAAACCA ATTCCACAGA GCAGGAAAGG CTCTAATAAC 
TCAAATAGGC CACCCGTGAT CCCATTGGGG ACGCAGGAGC CTCGATCTTC GAGAACTAAC 
TCAGCGATCT CGCAATCTCC CGTTAATTAT GCTTTCCCTA ACCCATACAA AATTCAACAA 
CTACAACAGG CTCCTATCCA ATCAGGTATG CCTTTACCAA ATACCAACAT ACCTCCCCCA 
GCATTAAAAG TGGAAACCAC CGTTTCTGCT CCTCCAATTC GGGCAAGAGG GGTCAGCAAT 
GCCTCCGTGG GAAGTTCAGC GTCTTTTGGT GCTAGACATG CAACACAGTA CGGCCTCAAT 
AACGGGGTAC CTCCGGTTTC GCCATATGGT CAAGCTACCA TAAATTTGCC AACTGCGAAT 
AAGTATGCCC CCGTCTCTCC TACAGTTCAG CAGAAGCAAT ATCCATCAGT TGTGCAAAAC 
CTTGGCGCTT CGGCCGTAAA TACCCCCAAT TTTGTAAAGA CCCATAGAGG CCATACAAGC 
TCTATTAGTT CGTATACACC AAACCAGAAT GAACACGCCT CTAGATACGC ACCCAACTAT 
CAACAATCTT ATCAGGTGCC ATATACCTCA CAACCTGTTG GTCCTGTAGC TGGGAATTCA 
AGCTATCAAA GCCAAACCCG AAGTTCTTAT GCAGTTCCTA TGATGCCCCA GGCTCAAACT 
TCAGCAAGTA TTCAGCCTCA CGCGAACATT CAACCGCCTA CTGGCATTTT ACCTTTAGCC 
CCCTTACGAC CTCTAGACCC CTTACAAGCC GCTACGAACT TGCAACCCCG TGCAAGCAAC 
ATAACGGCTG CAAATTCACT ACCTCTTGCA AATTTGCCAC TTGCTGAAAA CATACTGCCA 
GAAATTATCA CGCATCGAGC TACAAGTAGT GTTGCACCAC CACGACAGGA AAATAATCCA 
ATTAAAATAG ACAACGAGGC TTTATTACGC CGTCAATTTC CGATTTTTCA TTGGAGTGCT 
GCAAACAAGG TCGTGTACGC AGTCCCCCCT ATCCCTGACC AATCGCAGTA CATGATTTCA 
TCAAGCATTG TACAGGAAAT AAAAGTGACA CCAATTGACC AGATAATTAA ACCGAACGAT 
ATGCTCAAAA GCTTCCCAGG TCCTTTGGGT AGTGCCAAAT TAAAAAAAAA GGATTTAACC 
AAATGGATGG AAACCACTAT TAAATCCATA TCTGAAAATG AATCATCCAC TGATATGACT 
ATATGGCAAC TATTGGAAAT GAAACTAAAC GATAAAGTTA ACTGGAAAAA TATTTCAAAA 
CTACTATACA ATTCTGACGA ACTTTTAATG TACCTATCTC AGCCCTTTCC AAACGGTGAC 
ATGATTCCAA ATGCATATAG ACTGGATATA AATTGTCAGA TGAGAGTCCT GGCGTTCTTA 
CAAACGGGAA ATCACGATGA GGCACTTCGC TTAGCTTTAA GCAAGAGGGA TTATGCCATT 
GCACTATTGG TTGGCAGTTT AATGGGTAAA GACAGATGGT CTGAAGTCAT TCAGAAATAT 
TTATATGAAG GGTTTACTGC GGGGCCAAAC GACCAAAAAG AATTGGCACA CTTTCTGCTC 
CTTATCTTTC AAGTATTTGT TGGTAACTCC AAAATGGCCA TAAAAAGTTT CTACACTAAT 
AATGAGACCA GTCAATGGGC ATCCGAAAAC TGGAAGAGTA TCGTTGCAGC TGTTCTGATT 
AATATCCCAG AAAATAATGA AGATCCACTA CTTATACCAC CTGTTGTCCT TGAATTTTTG 
ATAGAGTTCG GTATATTCCT CACCAAAAAG GGCTTGACAG CCGCAGCTAG TACATTATTT 
ATTATTGGTA ACGTACCACT TTCTAATGAG CCAGTAATGG CAGATTCAGA CGTTATATTT 
GAAAGTATTG GAAACATGAA TACTTTTGAA AGCATTCTAT GGGATGAAAT CTACGAGTAT 
ATATTCTCGT ATGACCCTAA ATTCAAAGGA TTTTCATCTA TTTTGCCCCA GAAGATATAC 
CATGCATCTC TTTTACAAGA ACAAGGTTTG AACAGCCTGG GGACAAAGTA TACTGATTAC 
CTCAGTTCCT CAGTTCGAAA ACTGCCTAAG AAAGATATTT TAACAATAAA CCTCACTCGT 
GAATTGAGTG AGGTGGCTAG TAGGCTTTCC GAGTCTAATA CAGGATGGCT TGCAAAACCA 
AAACTAAGCA GCGTATGGGG TCAATTAGAT AAATCCTTCA ATAAATATAT TGGTGGCGAT 
GATATTGATG CATTGAATAA AAAAAATGAT AAAAAGAAAG TTTTTGATGG GTTCACACCG 
GGATCTTCTG CCAATTCGTC AACTGTGGAT CTCACCCAAA CATTCACACC TTTCCAAGCT 
CAAGTTACTT CGCAAAGCTA TGTGGATACT ACAGCTCTTT TGCATAATGC CCATAATGTA 
CCAAGCCATA GTGTGCTGCA TTCAAAGCCT TCCAATGTGT CAAAGGGGTT AGTTGAAGCA 
AACTTACCGT ATACGCATAG GATCGGTGAT AGTTTGCAGG GATCTCCTCA GCGCATTCAT 
AATACACAGT TCGCTGCTGC TGAGCCTCAA ATGGCTTCTT TGAGAAGAGT TAGAACAGAC 
CAGCATACAA ACGAAAAGGC TTTGAAGAGT CAGCAGATTT TAGAGAAAAA GTCTACGGCC 
TACACTCCAC AATTTGGACA GAACCATAGC GTTCCAATGG AAAAGTCTAA TTCGAATGTG 
CCATCTTTAT TTGCCGACTT CCCTGCTCCA CCCAAACTTG GAACAGTGCC GTCTAATTAT 
GTGTCTAGTC CTGACTTAGT AAGAAGGGAG TCTATCATAT CTACCGGATC AGAATTTCTT 
CCTCCTCCCA AAATTGGGGT ACCTACTAAA GCTAATTCCT CGCAGGGATC GCTTATGTAC 
TCACCAAGTG TGGAAGCTTT GCCTATCGAC CCTGTCGTCC CGCAAGTTCA TGAGACGGGA 
TACAATGATT TTGGTAACAA ACATTCTCAA AAAAGTATGC CTGAAGATGA ATCTCACACA 
TCACATGATA ATAGCAATGC TGATCAAAAT ACATTAAAAG ACTCTGCAGA TGTTACAGAT 
GAAACAATGG ATATTGAAGG ACCTGGCTTC AACGATGTGA AGAATCTTCT TCCTATGGAG 
CCCAACCACC AGCCTACGTC TACAGTAAAT CCTATACAAA CTATTAGTGA CGATATCCAA 
CCGATTCTTC AAACTAACGT GGAGGTCCGG GGTACTGATG CATCGAAAAT GGAAAATTCA 
CTTCCCTCCA TTGAAAATGA AAGAAGTAGC GAGGAGCAGC CAGAAAACAT TTCAAAATCA 
GCATCATCAG CATATTTACC ATCAACTGGT GGATTGTCAC TCGAAAACAG ACCGCTAACT 
CAGGATGAAA ACAGTATCTC AGAGACAGTT CAATCCACAT ACTTGCCAGC AGGAAGTATT 
TCAATGGAAG CTAAACCAAT TTCTCAAGTG CAAGATGTTC CAAGAAATGT TAATAATAAA 
GCATCCAAAC TTGTGGAGCA ACATATGGCA CCACCAAAGC CAAAAAGTAC TGACGCAACC 
AAAATGAACT ACTCACCATA CGTGCCTCAA TCAACTGCCG CTAGTGCAGA TGGCGATGAA 
TCAACGATTC TGAAAACATC GCCTGCTATA TATGCAAGAA CTCACCAAGC ACATGCATCC 
AATCCATCAC AATACTTTCC TTTGGTCAAC CAAGCAAATG AAACTGCTTC ATTCGAATTA 
TCTGAATCAA CATCCCAGGC ACAAAGTAAT GGAAATGTTG CTTCAGAAAA TAGATTCAGC 
CCAATAAAGA AAGCCGAAGT CGTCGAGAAA GACACTTTTC AACCTACTAT TAGGAAGGCT 
TCAACTAACC AATACAGGGC TTTTAAACCG TTGGAATCAG ATGCGGATAA ATACAATGAC 
GTTATTGAAG ATGAATCCGA TGACGACAAT ATGTCTACTG ATGAGGCAAA GAACAGAAAG 
GAAGAAAAAA AGAATGTGAA TATGAAAAAG GAAACAAAAC CAAGTAACAA GGACATAGAT 
GACAAGTCTA ATGGTTGGTT TGGTTGGTTG AAGAAAGATA CTGGCGACAA AAAAGTGTAT 
AAGGCCAAGC TAGGTCATAA AAACACACTA TACTATGATG AAAAATTGAA ACGTTGGGTG 
AATAAGGACG CAACCGAAGA GGAAAAACAA AAAATTATTG AAAGTTCGGC ACCACCACCT 
CCTCCAATCG TGAAACGTAA AGATGGCGGC CCAAAGACAA AGCCACGTTC AGGCCCCATC 
AATAATTCCC TACCTCCAGT ACATGCCACA TCAGTTATTC CGAACAATCC AATCACTGGT 
GAGCCTTTGC CGATCAAAAC ATCCCCTTCT CCTACAGGAC CCAATCCAAA CAATTCTCCA 
TCACCATCCT CTCCCATATC AAGGATTTCT GGCGTAAACT TGACTAGCAA AAAGGCAAAC 
GGTTTGGATG ATTTATTGAG TTTGGCAGGA GGACCCAAAC CAGCAAGTAC GAGAAGGAAG 
AAGAAAACAG CGAGAGGCTA TGTTAATGTA ATGGATAACA TACAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_015240.1
CDS	1..6588
Translation	MTPEAKKRKNQKKKLKQKQKKAAEKAASHSEEPLELPESTINSSFNDDSVNRTESDIASKSDVPPVSSSTNISPANETQLEIPDTQELHHKLLNDSDQHDITADSNDLPDNSIVEHDSVITQTKPAMSQEYEETAAHLSSRNPSLDVVAGELHNNNEHTQKIAVSAVEEDSFNEEEGENHDSIIISSLNDATPSQYNHFLPSDGNLLSPELSSGDTPTHNVPLGTKDNEINDDEYCNDKEISLNANNVLPDELSKEEDERLKLETHVSTEEKKQDIADQETAENLFTSSTEPSENKIRNSGDDTSMLFQDDESDQKVPWEEDVKKDFHNENTNNTQESAPNTDDRDKGYEGNEALKKSESCTAADERSYSEETSEDIFHGHDKQVVEGQNDFTGKNIENESQKLMGEGNHKLPLSAEADIIEPGKDIQDQAEDLFTQSSGDLGEVLPWESTDKNADVTSKSQEKHEDLFAASGNDEKLPWEVSDGEVSSGKTENSMQTSTEKIAEQKFSFLENDDDLLDDDDSFLASSEEEDTVPNTDNTTNLTSKPVEEKKASRYKPIIEEEAGMRQEQVHFTNTTGIVTPQQFHGLTKTGLGTPNQQVSVPNIVSPKPPVVKDNRSNFKINEEKKKSDAYDFPLEIISESSKKGHAKPVAVPTQRFGSGNSFSSLDKPIPQSRKGSNNSNRPPVIPLGTQEPRSSRTNSAISQSPVNYAFPNPYKIQQLQQAPIQSGMPLPNTNIPPPALKVETTVSAPPIRARGVSNASVGSSASFGARHATQYGLNNGVPPVSPYGQATINLPTANKYAPVSPTVQQKQYPSVVQNLGASAVNTPNFVKTHRGHTSSISSYTPNQNEHASRYAPNYQQSYQVPYTSQPVGPVAGNSSYQSQTRSSYAVPMMPQAQTSASIQPHANIQPPTGILPLAPLRPLDPLQAATNLQPRASNITAANSLPLANLPLAENILPEIITHRATSSVAPPRQENNPIKIDNEALLRRQFPIFHWSAANKVVYAVPPIPDQSQYMISSSIVQEIKVTPIDQIIKPNDMLKSFPGPLGSAKLKKKDLTKWMETTIKSISENESSTDMTIWQLLEMKLNDKVNWKNISKLLYNSDELLMYLSQPFPNGDMIPNAYRLDINCQMRVLAFLQTGNHDEALRLALSKRDYAIALLVGSLMGKDRWSEVIQKYLYEGFTAGPNDQKELAHFLLLIFQVFVGNSKMAIKSFYTNNETSQWASENWKSIVAAVLINIPENNEDPLLIPPVVLEFLIEFGIFLTKKGLTAAASTLFIIGNVPLSNEPVMADSDVIFESIGNMNTFESILWDEIYEYIFSYDPKFKGFSSILPQKIYHASLLQEQGLNSLGTKYTDYLSSSVRKLPKKDILTINLTRELSEVASRLSESNTGWLAKPKLSSVWGQLDKSFNKYIGGDDIDALNKKNDKKKVFDGFTPGSSANSSTVDLTQTFTPFQAQVTSQSYVDTTALLHNAHNVPSHSVLHSKPSNVSKGLVEANLPYTHRIGDSLQGSPQRIHNTQFAAAEPQMASLRRVRTDQHTNEKALKSQQILEKKSTAYTPQFGQNHSVPMEKSNSNVPSLFADFPAPPKLGTVPSNYVSSPDLVRRESIISTGSEFLPPPKIGVPTKANSSQGSLMYSPSVEALPIDPVVPQVHETGYNDFGNKHSQKSMPEDESHTSHDNSNADQNTLKDSADVTDETMDIEGPGFNDVKNLLPMEPNHQPTSTVNPIQTISDDIQPILQTNVEVRGTDASKMENSLPSIENERSSEEQPENISKSASSAYLPSTGGLSLENRPLTQDENSISETVQSTYLPAGSISMEAKPISQVQDVPRNVNNKASKLVEQHMAPPKPKSTDATKMNYSPYVPQSTAASADGDESTILKTSPAIYARTHQAHASNPSQYFPLVNQANETASFELSESTSQAQSNGNVASENRFSPIKKAEVVEKDTFQPTIRKASTNQYRAFKPLESDADKYNDVIEDESDDDNMSTDEAKNRKEEKKNVNMKKETKPSNKDIDDKSNGWFGWLKKDTGDKKVYKAKLGHKNTLYYDEKLKRWVNKDATEEEKQKIIESSAPPPPPIVKRKDGGPKTKPRSGPINNSLPPVHATSVIPNNPITGEPLPIKTSPSPTGPNPNNSPSPSSPISRISGVNLTSKKANGLDDLLSLAGGPKPASTRRKKKTARGYVNVMDNIQ

Target ORF information:

RefSeq Version	NM_001183899.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Sec16p (SEC16), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001183899.1

ORF Insert Sequence:

ATGACACCTG AAGCCAAGAA AAGGAAAAAC CAAAAGAAGA AGTTGAAGCA AAAGCAAAAA 
AAAGCTGCTG AGAAAGCTGC TAGCCACAGT GAAGAACCAC TTGAATTACC AGAAAGTACG 
ATTAACAGCA GCTTCAACGA CGACTCGGTG AACCGTACAG AATCTGACAT AGCTTCAAAA 
TCTGATGTTC CTCCGGTCTC ATCATCTACC AATATCTCTC CGGCTAATGA AACACAACTA 
GAAATACCTG ATACTCAAGA ATTGCATCAT AAACTGCTCA ACGACTCTGA TCAACATGAT 
ATTACCGCGG ACTCAAATGA TTTGCCAGAC AACTCAATCG TTGAACATGA CTCTGTTATT 
ACCCAAACAA AACCAGCCAT GTCTCAAGAA TACGAAGAGA CTGCCGCTCA CTTATCTTCG 
AGAAATCCAT CGCTCGATGT AGTCGCGGGA GAACTTCACA ATAATAATGA ACATACCCAG 
AAAATTGCCG TATCCGCTGT GGAAGAGGAT TCTTTCAATG AAGAAGAGGG TGAAAATCAC 
GACAGCATAA TAATTTCATC ATTAAACGAT GCTACCCCTT CTCAATATAA TCATTTTCTC 
CCATCCGATG GCAATCTTCT TTCTCCAGAA TTATCTTCTG GTGATACGCC AACTCACAAT 
GTTCCTCTAG GCACAAAAGA CAATGAAATA AATGACGATG AGTATTGTAA TGATAAGGAA 
ATTAGTTTGA ACGCAAATAA TGTGCTTCCT GATGAACTTT CAAAGGAAGA AGATGAAAGA 
TTAAAACTAG AAACGCATGT ATCAACCGAA GAAAAGAAAC AGGATATCGC TGATCAGGAA 
ACTGCAGAAA ACTTATTTAC GTCTAGTACA GAACCATCTG AGAATAAAAT AAGAAATTCT 
GGTGATGATA CCTCCATGTT GTTTCAAGAT GACGAAAGTG ATCAGAAGGT TCCATGGGAG 
GAAGATGTGA AGAAAGATTT TCATAATGAG AACACAAATA ATACTCAAGA ATCGGCACCG 
AACACAGATG ATCGTGATAA GGGTTATGAA GGAAACGAAG CTTTGAAAAA GTCCGAAAGT 
TGTACAGCCG CGGACGAGAG GTCGTACTCT GAAGAAACTT CAGAAGATAT CTTTCACGGA 
CACGACAAAC AGGTAGTTGA AGGCCAAAAT GATTTCACTG GGAAAAATAT TGAGAATGAA 
AGCCAGAAAT TAATGGGGGA AGGGAATCAT AAGTTACCGT TGTCTGCCGA AGCTGACATT 
ATAGAACCTG GTAAGGATAT TCAAGATCAA GCCGAGGATT TGTTTACGCA GAGCAGCGGA 
GACTTGGGAG AAGTTTTGCC ATGGGAATCT ACTGATAAAA ACGCTGATGT AACGAGCAAA 
TCCCAAGAGA AACATGAAGA TTTATTTGCT GCTTCTGGAA ACGATGAGAA ACTTCCTTGG 
GAAGTTTCTG ACGGTGAAGT ATCATCGGGA AAGACGGAAA ACAGCATGCA GACTAGTACT 
GAGAAAATAG CTGAGCAAAA GTTTTCGTTT TTGGAAAACG ACGACGACCT TTTGGACGAC 
GACGACAGCT TTTTGGCTTC TTCTGAGGAA GAAGACACAG TACCTAATAC CGATAATACA 
ACGAATTTAA CCTCAAAACC AGTTGAAGAA AAAAAGGCTT CAAGATATAA ACCTATTATC 
GAGGAGGAAG CAGGAATGCG TCAAGAGCAA GTTCATTTTA CCAATACTAC TGGCATTGTA 
ACACCGCAGC AGTTCCACGG TTTGACTAAA ACTGGACTAG GCACCCCCAA CCAACAAGTC 
AGTGTACCAA ATATAGTTAG TCCTAAGCCT CCTGTGGTAA AAGACAATCG TTCAAATTTT 
AAGATAAATG AGGAGAAAAA GAAGTCTGAT GCTTACGATT TTCCACTGGA AATTATTTCA 
GAAAGTTCCA AGAAGGGTCA CGCAAAGCCG GTTGCCGTTC CTACTCAAAG GTTTGGCTCA 
GGGAATTCTT TTAGTTCTTT GGACAAACCA ATTCCACAGA GCAGGAAAGG CTCTAATAAC 
TCAAATAGGC CACCCGTGAT CCCATTGGGG ACGCAGGAGC CTCGATCTTC GAGAACTAAC 
TCAGCGATCT CGCAATCTCC CGTTAATTAT GCTTTCCCTA ACCCATACAA AATTCAACAA 
CTACAACAGG CTCCTATCCA ATCAGGTATG CCTTTACCAA ATACCAACAT ACCTCCCCCA 
GCATTAAAAG TGGAAACCAC CGTTTCTGCT CCTCCAATTC GGGCAAGAGG GGTCAGCAAT 
GCCTCCGTGG GAAGTTCAGC GTCTTTTGGT GCTAGACATG CAACACAGTA CGGCCTCAAT 
AACGGGGTAC CTCCGGTTTC GCCATATGGT CAAGCTACCA TAAATTTGCC AACTGCGAAT 
AAGTATGCCC CCGTCTCTCC TACAGTTCAG CAGAAGCAAT ATCCATCAGT TGTGCAAAAC 
CTTGGCGCTT CGGCCGTAAA TACCCCCAAT TTTGTAAAGA CCCATAGAGG CCATACAAGC 
TCTATTAGTT CGTATACACC AAACCAGAAT GAACACGCCT CTAGATACGC ACCCAACTAT 
CAACAATCTT ATCAGGTGCC ATATACCTCA CAACCTGTTG GTCCTGTAGC TGGGAATTCA 
AGCTATCAAA GCCAAACCCG AAGTTCTTAT GCAGTTCCTA TGATGCCCCA GGCTCAAACT 
TCAGCAAGTA TTCAGCCTCA CGCGAACATT CAACCGCCTA CTGGCATTTT ACCTTTAGCC 
CCCTTACGAC CTCTAGACCC CTTACAAGCC GCTACGAACT TGCAACCCCG TGCAAGCAAC 
ATAACGGCTG CAAATTCACT ACCTCTTGCA AATTTGCCAC TTGCTGAAAA CATACTGCCA 
GAAATTATCA CGCATCGAGC TACAAGTAGT GTTGCACCAC CACGACAGGA AAATAATCCA 
ATTAAAATAG ACAACGAGGC TTTATTACGC CGTCAATTTC CGATTTTTCA TTGGAGTGCT 
GCAAACAAGG TCGTGTACGC AGTCCCCCCT ATCCCTGACC AATCGCAGTA CATGATTTCA 
TCAAGCATTG TACAGGAAAT AAAAGTGACA CCAATTGACC AGATAATTAA ACCGAACGAT 
ATGCTCAAAA GCTTCCCAGG TCCTTTGGGT AGTGCCAAAT TAAAAAAAAA GGATTTAACC 
AAATGGATGG AAACCACTAT TAAATCCATA TCTGAAAATG AATCATCCAC TGATATGACT 
ATATGGCAAC TATTGGAAAT GAAACTAAAC GATAAAGTTA ACTGGAAAAA TATTTCAAAA 
CTACTATACA ATTCTGACGA ACTTTTAATG TACCTATCTC AGCCCTTTCC AAACGGTGAC 
ATGATTCCAA ATGCATATAG ACTGGATATA AATTGTCAGA TGAGAGTCCT GGCGTTCTTA 
CAAACGGGAA ATCACGATGA GGCACTTCGC TTAGCTTTAA GCAAGAGGGA TTATGCCATT 
GCACTATTGG TTGGCAGTTT AATGGGTAAA GACAGATGGT CTGAAGTCAT TCAGAAATAT 
TTATATGAAG GGTTTACTGC GGGGCCAAAC GACCAAAAAG AATTGGCACA CTTTCTGCTC 
CTTATCTTTC AAGTATTTGT TGGTAACTCC AAAATGGCCA TAAAAAGTTT CTACACTAAT 
AATGAGACCA GTCAATGGGC ATCCGAAAAC TGGAAGAGTA TCGTTGCAGC TGTTCTGATT 
AATATCCCAG AAAATAATGA AGATCCACTA CTTATACCAC CTGTTGTCCT TGAATTTTTG 
ATAGAGTTCG GTATATTCCT CACCAAAAAG GGCTTGACAG CCGCAGCTAG TACATTATTT 
ATTATTGGTA ACGTACCACT TTCTAATGAG CCAGTAATGG CAGATTCAGA CGTTATATTT 
GAAAGTATTG GAAACATGAA TACTTTTGAA AGCATTCTAT GGGATGAAAT CTACGAGTAT 
ATATTCTCGT ATGACCCTAA ATTCAAAGGA TTTTCATCTA TTTTGCCCCA GAAGATATAC 
CATGCATCTC TTTTACAAGA ACAAGGTTTG AACAGCCTGG GGACAAAGTA TACTGATTAC 
CTCAGTTCCT CAGTTCGAAA ACTGCCTAAG AAAGATATTT TAACAATAAA CCTCACTCGT 
GAATTGAGTG AGGTGGCTAG TAGGCTTTCC GAGTCTAATA CAGGATGGCT TGCAAAACCA 
AAACTAAGCA GCGTATGGGG TCAATTAGAT AAATCCTTCA ATAAATATAT TGGTGGCGAT 
GATATTGATG CATTGAATAA AAAAAATGAT AAAAAGAAAG TTTTTGATGG GTTCACACCG 
GGATCTTCTG CCAATTCGTC AACTGTGGAT CTCACCCAAA CATTCACACC TTTCCAAGCT 
CAAGTTACTT CGCAAAGCTA TGTGGATACT ACAGCTCTTT TGCATAATGC CCATAATGTA 
CCAAGCCATA GTGTGCTGCA TTCAAAGCCT TCCAATGTGT CAAAGGGGTT AGTTGAAGCA 
AACTTACCGT ATACGCATAG GATCGGTGAT AGTTTGCAGG GATCTCCTCA GCGCATTCAT 
AATACACAGT TCGCTGCTGC TGAGCCTCAA ATGGCTTCTT TGAGAAGAGT TAGAACAGAC 
CAGCATACAA ACGAAAAGGC TTTGAAGAGT CAGCAGATTT TAGAGAAAAA GTCTACGGCC 
TACACTCCAC AATTTGGACA GAACCATAGC GTTCCAATGG AAAAGTCTAA TTCGAATGTG 
CCATCTTTAT TTGCCGACTT CCCTGCTCCA CCCAAACTTG GAACAGTGCC GTCTAATTAT 
GTGTCTAGTC CTGACTTAGT AAGAAGGGAG TCTATCATAT CTACCGGATC AGAATTTCTT 
CCTCCTCCCA AAATTGGGGT ACCTACTAAA GCTAATTCCT CGCAGGGATC GCTTATGTAC 
TCACCAAGTG TGGAAGCTTT GCCTATCGAC CCTGTCGTCC CGCAAGTTCA TGAGACGGGA 
TACAATGATT TTGGTAACAA ACATTCTCAA AAAAGTATGC CTGAAGATGA ATCTCACACA 
TCACATGATA ATAGCAATGC TGATCAAAAT ACATTAAAAG ACTCTGCAGA TGTTACAGAT 
GAAACAATGG ATATTGAAGG ACCTGGCTTC AACGATGTGA AGAATCTTCT TCCTATGGAG 
CCCAACCACC AGCCTACGTC TACAGTAAAT CCTATACAAA CTATTAGTGA CGATATCCAA 
CCGATTCTTC AAACTAACGT GGAGGTCCGG GGTACTGATG CATCGAAAAT GGAAAATTCA 
CTTCCCTCCA TTGAAAATGA AAGAAGTAGC GAGGAGCAGC CAGAAAACAT TTCAAAATCA 
GCATCATCAG CATATTTACC ATCAACTGGT GGATTGTCAC TCGAAAACAG ACCGCTAACT 
CAGGATGAAA ACAGTATCTC AGAGACAGTT CAATCCACAT ACTTGCCAGC AGGAAGTATT 
TCAATGGAAG CTAAACCAAT TTCTCAAGTG CAAGATGTTC CAAGAAATGT TAATAATAAA 
GCATCCAAAC TTGTGGAGCA ACATATGGCA CCACCAAAGC CAAAAAGTAC TGACGCAACC 
AAAATGAACT ACTCACCATA CGTGCCTCAA TCAACTGCCG CTAGTGCAGA TGGCGATGAA 
TCAACGATTC TGAAAACATC GCCTGCTATA TATGCAAGAA CTCACCAAGC ACATGCATCC 
AATCCATCAC AATACTTTCC TTTGGTCAAC CAAGCAAATG AAACTGCTTC ATTCGAATTA 
TCTGAATCAA CATCCCAGGC ACAAAGTAAT GGAAATGTTG CTTCAGAAAA TAGATTCAGC 
CCAATAAAGA AAGCCGAAGT CGTCGAGAAA GACACTTTTC AACCTACTAT TAGGAAGGCT 
TCAACTAACC AATACAGGGC TTTTAAACCG TTGGAATCAG ATGCGGATAA ATACAATGAC 
GTTATTGAAG ATGAATCCGA TGACGACAAT ATGTCTACTG ATGAGGCAAA GAACAGAAAG 
GAAGAAAAAA AGAATGTGAA TATGAAAAAG GAAACAAAAC CAAGTAACAA GGACATAGAT 
GACAAGTCTA ATGGTTGGTT TGGTTGGTTG AAGAAAGATA CTGGCGACAA AAAAGTGTAT 
AAGGCCAAGC TAGGTCATAA AAACACACTA TACTATGATG AAAAATTGAA ACGTTGGGTG 
AATAAGGACG CAACCGAAGA GGAAAAACAA AAAATTATTG AAAGTTCGGC ACCACCACCT 
CCTCCAATCG TGAAACGTAA AGATGGCGGC CCAAAGACAA AGCCACGTTC AGGCCCCATC 
AATAATTCCC TACCTCCAGT ACATGCCACA TCAGTTATTC CGAACAATCC AATCACTGGT 
GAGCCTTTGC CGATCAAAAC ATCCCCTTCT CCTACAGGAC CCAATCCAAA CAATTCTCCA 
TCACCATCCT CTCCCATATC AAGGATTTCT GGCGTAAACT TGACTAGCAA AAAGGCAAAC 
GGTTTGGATG ATTTATTGAG TTTGGCAGGA GGACCCAAAC CAGCAAGTAC GAGAAGGAAG 
AAGAAAACAG CGAGAGGCTA TGTTAATGTA ATGGATAACA TACAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
Nature387(6632 Suppl)103-5(1997 May)
Bussey H,Storms RK,Ahmed A,Albermann K,Allen E,Ansorge W,Araujo R,Aparicio A,Barrell B,Badcock K,Benes V,Botstein D,Bowman S,Br?ckner M,Carpenter J,Cherry JM,Chung E,Churcher C,Coster F,Davis K,Davis RW,Dietrich FS,Delius H,DiPaolo T,Hani J

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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SEC16 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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