TBF1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	TBF1
Entrez Gene ID	855975
Full Name	Tbf1p
Synonyms	LPI16
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001183942.1	NP_015197.1	Tbf1p

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_ACR096W	NP_983498.1
Saccharomyces cerevisiae (baker's yeast)	TBF1	NP_015197.1
Kluyveromyces lactis	KLLA0D06765g	XP_453362.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
Bussey H, Storms RK, Ahmed A, Albermann K, Allen E, Ansorge W, Araujo R, Aparicio A, Barrell B, Badcock K, Benes V, Botstein D, Bowman S, Br?ckner M, Carpenter J, Cherry JM, Chung E, Churcher C, Coster F, Davis K, Davis RW, Dietrich FS, Delius H, DiPaolo T, Hani J
Nature387(6632 Suppl)103-5(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Abf1, Reb1, and Tbf1 control ribosome biogenesis gene expression in budding yeast.
Title: Abf1 and other general regulatory factors control ribosome biogenesis gene expression in budding yeast.

Tbf1 and Vid22 promote resection and non-homologous end joining of DNA double-strand break ends.
Title: Tbf1 and Vid22 promote resection and non-homologous end joining of DNA double-strand break ends.

These data support a model in which Tbf1 and Rap1 collaborate to maintain genomic stability of short telomeres.
Title: Subtelomere-binding protein Tbf1 and telomere-binding protein Rap1 collaborate to inhibit localization of the Mre11 complex to DNA ends in budding yeast.

Tbf1 capping of DNA is an evolved function.
Title: DNA-end capping by the budding yeast transcription factor and subtelomeric binding protein Tbf1.

Fission and budding yeast Tbf1 proteins have diverged significantly in their functions.
Title: Functional differentiation of tbf1 orthologues in fission and budding yeasts.

The following TBF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TBF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04844	NM_001183942.1 Latest version!	Saccharomyces cerevisiae S288C Tbf1p (TBF1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TBF1 ( NM_001183942.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_015197.1 Saccharomyces cerevisiae S288C Tbf1p (TBF1), partial mRNA.

Price & Availability↑

CloneID	OSi04844
Clone ID Related Accession (Same CDS sequence)	NM_001183942.1
Accession Version	NM_001183942.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1689bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Tbf1p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001148). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGATTCGC AAGTGCCCAA TAATAACGAA AGCTTAAACC GCTTCAATGA CATCATCCAA 
AGCCTGCCTG CAAGAACTAG GTTGACCATA TGCTCATTAT GTCTGCTGGA CAATATCTCC 
ACTCAACTAC TGAGGTTTCT TATCCTTAAT GCCAACTCTC CCAATATTAT AGCGGTTTTG 
ACGGACCAGA CGGCTTTCCT AAGCTCAGGC GAAACAGAAA TTTTTCAAAC GTTAGTCAAG 
CTCTTTAAGC AGATCAGAAT GATTTACCAC ACCAGATCTC CGTTACTGTC TGTTCACGAC 
GTGGCGCCTG GTTTATGGTT TCCCAACTCC CCTCCCCCCC TTATATTGAG AGGCCACGAA 
GCGTTTATTA TTACTGCCAT AAGAAAGGCG AATCTGCTAA CATTTCTATT GACGTCGCTG 
AACTGCCTTA ATTACGGGTT TGAGCTGCTA CAGTCGATAT TTCTCGATAT TTTCTGTCCA 
AATACAAACA CTGTCGGTAA CAACTCCCTG GAACAAAGCG GAAAGTTTTT GAAATCGCAG 
GCTATCCTAT ATCTTGATCT GAAAACACAG GCTTACATTG CCGGCTTGAA AGAGTTTCAG 
GACGAGACGA ACGAAATTTC TTTAGAAAAG AAACAAGAGC TACTAGACTT AATCTTCCCA 
TCCAATCTGG CTGATATTCT CGTGCAGAGA AGAACCGGCG ATTCTGGAGA CATTACACTT 
TTGACTCCAT CGGAGAAGGA CTTCGTCGAA AGGTGCGATC GTCGGAGAGA AAATTTAAAG 
ATCGTCCAAG ATTTTAACTC TCTGACCCAG AGCTACGAAT GGGCTCAATT TATCAGAGAG 
TTGCTGGATT ATTGTAATAA AAATATGGGT CTCATCATAT GGGGCAGAAA GGGTCGCGGA 
AAATCACCAT TATATGACTT TGATGTCAAC GAGTTTGATC CTCAAGTACT CTTTTCTACA 
GGGACCAGGA CCGTAGAATT CATGGACGAC CAAAATCAGC CAAGCAGTGC GTCTGCTTTT 
TTGTCAACTG CTCGTCCGAA TCATTACAGC ACACATACAC CTACCACAGA TGTGTCTTCC 
AAAAACCCCG CTATCACACA ATCCATTGTG GATGCCGCTG TTGCGGCCTC GATGAGCAAC 
AGCAGCTCTG GTCCTCATTC CTCTCATAAC AACAGCAGTA ACAGCAACAA TAATGGTAGT 
ATCGGATTAA GAAAACCAAA GGCGAAAAGA ACTTGGTCCA AAGAGGAAGA AGAAGCATTA 
GTGGAAGGCC TGAAAGAGGT TGGCCCATCG TGGTCTAAGA TTCTAGATTT ATATGGTCCC 
GGTGGTAAAA TCACGGAAAA TTTGAAGAAC AGGACGCAGG TACAACTGAA AGATAAAGCC 
CGTAATTGGA AATTACAATA CTTGAAGAGT GGCAAACCAT TGCCTGACTA TTTGATAAAA 
GTGACCGGTA ACTTGGAGAA AATCTACAAG GCAAAGAAAA AATTCTCTCA ATCTCCAAAT 
TCGTCAACTA TTATGGAACA AAATTTATCT CAGCATCCTT CATCAGCTGC ATCTGCTACT 
GAAGACACTC AAACTCATCA AGAGGATTCA CATGGGCAAA ATAGTGATAA TATGCCATCT 
AACGGACTTT TTGGCAATAG TACATCGGAC AATACAGGTT TTGATCCTCA TTTAGAAGAT 
GGGATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_015197.1
CDS	1..1689
Translation	MDSQVPNNNESLNRFNDIIQSLPARTRLTICSLCLLDNISTQLLRFLILNANSPNIIAVLTDQTAFLSSGETEIFQTLVKLFKQIRMIYHTRSPLLSVHDVAPGLWFPNSPPPLILRGHEAFIITAIRKANLLTFLLTSLNCLNYGFELLQSIFLDIFCPNTNTVGNNSLEQSGKFLKSQAILYLDLKTQAYIAGLKEFQDETNEISLEKKQELLDLIFPSNLADILVQRRTGDSGDITLLTPSEKDFVERCDRRRENLKIVQDFNSLTQSYEWAQFIRELLDYCNKNMGLIIWGRKGRGKSPLYDFDVNEFDPQVLFSTGTRTVEFMDDQNQPSSASAFLSTARPNHYSTHTPTTDVSSKNPAITQSIVDAAVAASMSNSSSGPHSSHNNSSNSNNNGSIGLRKPKAKRTWSKEEEEALVEGLKEVGPSWSKILDLYGPGGKITENLKNRTQVQLKDKARNWKLQYLKSGKPLPDYLIKVTGNLEKIYKAKKKFSQSPNSSTIMEQNLSQHPSSAASATEDTQTHQEDSHGQNSDNMPSNGLFGNSTSDNTGFDPHLEDGM

Target ORF information:

RefSeq Version	NM_001183942.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Tbf1p (TBF1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001183942.1

ORF Insert Sequence:

ATGGATTCGC AAGTGCCCAA TAATAACGAA AGCTTAAACC GCTTCAATGA CATCATCCAA 
AGCCTGCCTG CAAGAACTAG GTTGACCATA TGCTCATTAT GTCTGCTGGA CAATATCTCC 
ACTCAACTAC TGAGGTTTCT TATCCTTAAT GCCAACTCTC CCAATATTAT AGCGGTTTTG 
ACGGACCAGA CGGCTTTCCT AAGCTCAGGC GAAACAGAAA TTTTTCAAAC GTTAGTCAAG 
CTCTTTAAGC AGATCAGAAT GATTTACCAC ACCAGATCTC CGTTACTGTC TGTTCACGAC 
GTGGCGCCTG GTTTATGGTT TCCCAACTCC CCTCCCCCCC TTATATTGAG AGGCCACGAA 
GCGTTTATTA TTACTGCCAT AAGAAAGGCG AATCTGCTAA CATTTCTATT GACGTCGCTG 
AACTGCCTTA ATTACGGGTT TGAGCTGCTA CAGTCGATAT TTCTCGATAT TTTCTGTCCA 
AATACAAACA CTGTCGGTAA CAACTCCCTG GAACAAAGCG GAAAGTTTTT GAAATCGCAG 
GCTATCCTAT ATCTTGATCT GAAAACACAG GCTTACATTG CCGGCTTGAA AGAGTTTCAG 
GACGAGACGA ACGAAATTTC TTTAGAAAAG AAACAAGAGC TACTAGACTT AATCTTCCCA 
TCCAATCTGG CTGATATTCT CGTGCAGAGA AGAACCGGCG ATTCTGGAGA CATTACACTT 
TTGACTCCAT CGGAGAAGGA CTTCGTCGAA AGGTGCGATC GTCGGAGAGA AAATTTAAAG 
ATCGTCCAAG ATTTTAACTC TCTGACCCAG AGCTACGAAT GGGCTCAATT TATCAGAGAG 
TTGCTGGATT ATTGTAATAA AAATATGGGT CTCATCATAT GGGGCAGAAA GGGTCGCGGA 
AAATCACCAT TATATGACTT TGATGTCAAC GAGTTTGATC CTCAAGTACT CTTTTCTACA 
GGGACCAGGA CCGTAGAATT CATGGACGAC CAAAATCAGC CAAGCAGTGC GTCTGCTTTT 
TTGTCAACTG CTCGTCCGAA TCATTACAGC ACACATACAC CTACCACAGA TGTGTCTTCC 
AAAAACCCCG CTATCACACA ATCCATTGTG GATGCCGCTG TTGCGGCCTC GATGAGCAAC 
AGCAGCTCTG GTCCTCATTC CTCTCATAAC AACAGCAGTA ACAGCAACAA TAATGGTAGT 
ATCGGATTAA GAAAACCAAA GGCGAAAAGA ACTTGGTCCA AAGAGGAAGA AGAAGCATTA 
GTGGAAGGCC TGAAAGAGGT TGGCCCATCG TGGTCTAAGA TTCTAGATTT ATATGGTCCC 
GGTGGTAAAA TCACGGAAAA TTTGAAGAAC AGGACGCAGG TACAACTGAA AGATAAAGCC 
CGTAATTGGA AATTACAATA CTTGAAGAGT GGCAAACCAT TGCCTGACTA TTTGATAAAA 
GTGACCGGTA ACTTGGAGAA AATCTACAAG GCAAAGAAAA AATTCTCTCA ATCTCCAAAT 
TCGTCAACTA TTATGGAACA AAATTTATCT CAGCATCCTT CATCAGCTGC ATCTGCTACT 
GAAGACACTC AAACTCATCA AGAGGATTCA CATGGGCAAA ATAGTGATAA TATGCCATCT 
AACGGACTTT TTGGCAATAG TACATCGGAC AATACAGGTT TTGATCCTCA TTTAGAAGAT 
GGGATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
Nature387(6632 Suppl)103-5(1997 May)
Bussey H,Storms RK,Ahmed A,Albermann K,Allen E,Ansorge W,Araujo R,Aparicio A,Barrell B,Badcock K,Benes V,Botstein D,Bowman S,Br?ckner M,Carpenter J,Cherry JM,Chung E,Churcher C,Coster F,Davis K,Davis RW,Dietrich FS,Delius H,DiPaolo T,Hani J

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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TBF1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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