RAD53 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	RAD53
Entrez Gene ID	855950
Full Name	serine/threonine/tyrosine protein kinase RAD53
Synonyms	LSD1,MEC2,SPK1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001183967.1	NP_015172.1	serine/threonine/tyrosine protein kinase RAD53

Pathways from BioSystems

Homologies

Homo sapiens (human)	CHEK2	NP_001005735.1
Rattus norvegicus (Norway rat)	Chek2	NP_446129.1
Saccharomyces cerevisiae (baker's yeast)	RAD53	NP_015172.1
Bos taurus (cattle)	CHEK2	NP_001029703.1
Gallus gallus (chicken)	CHEK2	NP_001073576.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009784	XP_318879.3
Caenorhabditis elegans (roundworm)	CELE_T08D2.7	NP_508060.1
Caenorhabditis elegans (roundworm)	chk-2	NP_001024271.1
Kluyveromyces lactis	KLLA0F11143g	XP_455587.1
	CHEK2	XP_001101658.1
Mus musculus (house mouse)	Chek2	NP_057890.1
Danio rerio (zebrafish)	chek2	NP_956339.1
Pan troglodytes (chimpanzee)	CHEK2	XP_003317205.1
Canis lupus familiaris (dog)	CHEK2	XP_005636447.1
Drosophila melanogaster (fruit fly)	lok	NP_724241.1
Eremothecium gossypii	AGOS_ACR142W	NP_983544.2
Xenopus tropicalis (tropical clawed frog)	chek2	NP_001119996.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
Bussey H, Storms RK, Ahmed A, Albermann K, Allen E, Ansorge W, Araujo R, Aparicio A, Barrell B, Badcock K, Benes V, Botstein D, Bowman S, Br?ckner M, Carpenter J, Cherry JM, Chung E, Churcher C, Coster F, Davis K, Davis RW, Dietrich FS, Delius H, DiPaolo T, Hani J
Nature387(6632 Suppl)103-5(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Rad53 prevents the generation of excessive ssDNA under replication stress by coordinating DNA unwinding with synthesis of both strands.
Title: Checkpoint Kinase Rad53 Couples Leading- and Lagging-Strand DNA Synthesis under Replication Stress.

Rad53 can display active conformations and phosphor-priming confers functionally relevant autophosphorylation.
Title: Phospho-Priming Confers Functionally Relevant Specificities for Rad53 Kinase Autophosphorylation.

By performing a genetic screen, we identify rad53 and tel1 mutant alleles that suppress both the DNA damage hypersensitivity and the resection defect of sae2Delta cells through an Sgs1-Dna2-dependent mechanism
Title: Sae2 Function at DNA Double-Strand Breaks Is Bypassed by Dampening Tel1 or Rad53 Activity.

Rad53-mediated regulation of Rrm3 and Pif1 DNA helicases contributes to prevention of aberrant fork transitions under replication stress.
Title: Rad53-Mediated Regulation of Rrm3 and Pif1 DNA Helicases Contributes to Prevention of Aberrant Fork Transitions under Replication Stress.

INO80 can activate Rad53 kinase activity in an Ies4-phosphorylation-dependent manner in the absence of known activators such as Rad9.
Title: Phosphorylation-Dependent Enhancement of Rad53 Kinase Activity through the INO80 Chromatin Remodeling Complex.

The following RAD53 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RAD53 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04821	NM_001183967.1 Latest version!	Saccharomyces cerevisiae S288C serine/threonine/tyrosine protein kinase RAD53 (RAD53), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RAD53 ( NM_001183967.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_015172.1 Saccharomyces cerevisiae S288C serine/threonine/tyrosine protein kinase RAD53 (RAD53), partial mRNA.

Price & Availability↑

CloneID	OSi04821
Clone ID Related Accession (Same CDS sequence)	NM_001183967.1
Accession Version	NM_001183967.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2466bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	serine/threonine/tyrosine protein kinase RAD53
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001148). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGAAAATA TTACACAACC CACACAGCAA TCCACGCAGG CTACTCAAAG GTTTTTGATT 
GAGAAGTTTT CTCAAGAACA GATCGGCGAA AACATTGTGT GCAGGGTCAT TTGTACCACG 
GGTCAAATTC CCATCCGAGA TTTGTCAGCT GATATTTCAC AAGTGCTTAA GGAAAAACGA 
TCCATAAAGA AAGTTTGGAC ATTTGGTAGA AACCCAGCCT GTGACTATCA TTTAGGAAAC 
ATTTCAAGAC TGTCAAATAA GCATTTCCAA ATACTACTAG GAGAAGACGG TAACCTTTTA 
TTGAATGACA TTTCCACTAA TGGGACCTGG TTAAATGGGC AAAAAGTCGA GAAGAACAGC 
AATCAGTTAC TGTCTCAAGG TGATGAAATA ACCGTTGGTG TAGGCGTGGA ATCAGATATT 
TTATCTCTGG TCATTTTCAT AAACGACAAA TTTAAGCAGT GCCTCGAGCA GAACAAAGTT 
GATCGCATAA GATCTAACCT GAAAAATACC TCTAAAATAG CTTCTCCTGG TCTTACATCA 
TCTACTGCAT CATCAATGGT GGCCAACAAG ACTGGTATTT TTAAGGATTT TTCGATTATT 
GACGAAGTGG TGGGCCAGGG TGCATTTGCC ACAGTAAAGA AAGCCATTGA AAGAACTACT 
GGGAAAACAT TCGCGGTGAA GATTATAAGT AAACGCAAAG TAATAGGCAA TATGGATGGT 
GTGACAAGAG AGTTAGAAGT ATTGCAAAAG CTCAATCATC CAAGGATAGT ACGATTGAAA 
GGATTTTATG AAGATACTGA GAGTTATTAT ATGGTGATGG AGTTCGTTTC TGGTGGTGAC 
TTAATGGATT TTGTTGCTGC TCATGGTGCG GTTGGAGAAG ATGCTGGGAG GGAGATATCC 
AGGCAGATAC TCACAGCAAT AAAATACATT CACTCTATGG GCATCAGCCA TCGTGACCTA 
AAGCCCGATA ATATTCTTAT TGAACAAGAC GATCCTGTAT TGGTAAAGAT AACCGACTTT 
GGTCTGGCAA AAGTACAAGG AAATGGGTCT TTTATGAAAA CCTTCTGTGG CACTTTGGCA 
TATGTGGCAC CTGAAGTCAT CAGAGGTAAA GATACATCCG TATCTCCTGA TGAATACGAA 
GAAAGGAATG AGTACTCTTC GTTAGTGGAT ATGTGGTCAA TGGGATGTCT TGTGTATGTT 
ATCCTAACGG GCCACTTACC TTTTAGTGGT AGCACACAGG ACCAATTATA TAAACAGATT 
GGAAGAGGCT CATATCATGA AGGGCCCCTC AAAGATTTCC GGATATCTGA AGAAGCAAGA 
GATTTCATAG ATTCATTGTT ACAGGTGGAT CCAAATAATA GGTCGACAGC TGCAAAAGCC 
TTGAATCATC CCTGGATCAA GATGAGTCCA TTGGGCTCAC AATCATATGG TGATTTTTCA 
CAAATATCCT TATCACAATC GTTGTCGCAG CAGAAATTAT TAGAAAATAT GGACGATGCT 
CAATACGAAT TTGTCAAAGC GCAAAGGAAA TTACAAATGG AGCAACAACT TCAAGAACAG 
GATCAGGAAG ACCAAGATGG AAAAATTCAA GGATTTAAAA TACCCGCACA CGCCCCTATT 
CGATATACAC AGCCCAAAAG CATTGAAGCA GAAACTAGAG AACAAAAACT TTTACATTCC 
AATAATACTG AGAATGTCAA GAGCTCAAAG AAAAAGGGTA ATGGTAGGTT TTTAACTTTA 
AAACCATTGC CTGACAGCAT TATTCAAGAA AGCCTGGAGA TTCAGCAAGG TGTGAATCCA 
TTTTTCATTG GTAGATCCGA GGATTGCAAT TGTAAAATTG AAGACAATAG GTTGTCTCGA 
GTTCATTGCT TCATTTTCAA AAAGAGGCAT GCTGTAGGCA AAAGCATGTA TGAATCTCCG 
GCACAAGGTT TAGATGATAT TTGGTATTGC CACACCGGAA CTAACGTGAG CTATTTAAAT 
AATAACCGCA TGATACAGGG TACGAAATTC CTTTTACAAG ACGGAGATGA AATCAAGATC 
ATTTGGGATA AAAACAATAA ATTTGTCATT GGCTTTAAAG TGGAAATTAA CGATACTACA 
GGTCTGTTTA ACGAGGGATT AGGTATGTTA CAAGAACAAA GAGTAGTACT TAAGCAAACA 
GCCGAAGAAA AAGATTTGGT GAAAAAGTTA ACCCAGATGA TGGCAGCTCA ACGTGCAAAT 
CAACCCTCGG CTTCTTCTTC ATCAATGTCG GCTAAGAAGC CGCCAGTTAG CGATACAAAT 
AATAACGGCA ATAATTCGGT ACTAAACGAC TTGGTAGAGT CACCGATTAA TGCGAATACG 
GGGAACATTT TGAAGAGAAT ACATTCGGTA AGTTTATCGC AATCACAAAT TGATCCTAGT 
AAGAAGGTTA AAAGGGCAAA ATTGGACCAA ACCTCAAAAG GCCCCGAGAA TTTGCAATTT 
TCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_015172.1
CDS	1..2466
Translation	MENITQPTQQSTQATQRFLIEKFSQEQIGENIVCRVICTTGQIPIRDLSADISQVLKEKRSIKKVWTFGRNPACDYHLGNISRLSNKHFQILLGEDGNLLLNDISTNGTWLNGQKVEKNSNQLLSQGDEITVGVGVESDILSLVIFINDKFKQCLEQNKVDRIRSNLKNTSKIASPGLTSSTASSMVANKTGIFKDFSIIDEVVGQGAFATVKKAIERTTGKTFAVKIISKRKVIGNMDGVTRELEVLQKLNHPRIVRLKGFYEDTESYYMVMEFVSGGDLMDFVAAHGAVGEDAGREISRQILTAIKYIHSMGISHRDLKPDNILIEQDDPVLVKITDFGLAKVQGNGSFMKTFCGTLAYVAPEVIRGKDTSVSPDEYEERNEYSSLVDMWSMGCLVYVILTGHLPFSGSTQDQLYKQIGRGSYHEGPLKDFRISEEARDFIDSLLQVDPNNRSTAAKALNHPWIKMSPLGSQSYGDFSQISLSQSLSQQKLLENMDDAQYEFVKAQRKLQMEQQLQEQDQEDQDGKIQGFKIPAHAPIRYTQPKSIEAETREQKLLHSNNTENVKSSKKKGNGRFLTLKPLPDSIIQESLEIQQGVNPFFIGRSEDCNCKIEDNRLSRVHCFIFKKRHAVGKSMYESPAQGLDDIWYCHTGTNVSYLNNNRMIQGTKFLLQDGDEIKIIWDKNNKFVIGFKVEINDTTGLFNEGLGMLQEQRVVLKQTAEEKDLVKKLTQMMAAQRANQPSASSSSMSAKKPPVSDTNNNGNNSVLNDLVESPINANTGNILKRIHSVSLSQSQIDPSKKVKRAKLDQTSKGPENLQFS

Target ORF information:

RefSeq Version	NM_001183967.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C serine/threonine/tyrosine protein kinase RAD53 (RAD53), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001183967.1

ORF Insert Sequence:

ATGGAAAATA TTACACAACC CACACAGCAA TCCACGCAGG CTACTCAAAG GTTTTTGATT 
GAGAAGTTTT CTCAAGAACA GATCGGCGAA AACATTGTGT GCAGGGTCAT TTGTACCACG 
GGTCAAATTC CCATCCGAGA TTTGTCAGCT GATATTTCAC AAGTGCTTAA GGAAAAACGA 
TCCATAAAGA AAGTTTGGAC ATTTGGTAGA AACCCAGCCT GTGACTATCA TTTAGGAAAC 
ATTTCAAGAC TGTCAAATAA GCATTTCCAA ATACTACTAG GAGAAGACGG TAACCTTTTA 
TTGAATGACA TTTCCACTAA TGGGACCTGG TTAAATGGGC AAAAAGTCGA GAAGAACAGC 
AATCAGTTAC TGTCTCAAGG TGATGAAATA ACCGTTGGTG TAGGCGTGGA ATCAGATATT 
TTATCTCTGG TCATTTTCAT AAACGACAAA TTTAAGCAGT GCCTCGAGCA GAACAAAGTT 
GATCGCATAA GATCTAACCT GAAAAATACC TCTAAAATAG CTTCTCCTGG TCTTACATCA 
TCTACTGCAT CATCAATGGT GGCCAACAAG ACTGGTATTT TTAAGGATTT TTCGATTATT 
GACGAAGTGG TGGGCCAGGG TGCATTTGCC ACAGTAAAGA AAGCCATTGA AAGAACTACT 
GGGAAAACAT TCGCGGTGAA GATTATAAGT AAACGCAAAG TAATAGGCAA TATGGATGGT 
GTGACAAGAG AGTTAGAAGT ATTGCAAAAG CTCAATCATC CAAGGATAGT ACGATTGAAA 
GGATTTTATG AAGATACTGA GAGTTATTAT ATGGTGATGG AGTTCGTTTC TGGTGGTGAC 
TTAATGGATT TTGTTGCTGC TCATGGTGCG GTTGGAGAAG ATGCTGGGAG GGAGATATCC 
AGGCAGATAC TCACAGCAAT AAAATACATT CACTCTATGG GCATCAGCCA TCGTGACCTA 
AAGCCCGATA ATATTCTTAT TGAACAAGAC GATCCTGTAT TGGTAAAGAT AACCGACTTT 
GGTCTGGCAA AAGTACAAGG AAATGGGTCT TTTATGAAAA CCTTCTGTGG CACTTTGGCA 
TATGTGGCAC CTGAAGTCAT CAGAGGTAAA GATACATCCG TATCTCCTGA TGAATACGAA 
GAAAGGAATG AGTACTCTTC GTTAGTGGAT ATGTGGTCAA TGGGATGTCT TGTGTATGTT 
ATCCTAACGG GCCACTTACC TTTTAGTGGT AGCACACAGG ACCAATTATA TAAACAGATT 
GGAAGAGGCT CATATCATGA AGGGCCCCTC AAAGATTTCC GGATATCTGA AGAAGCAAGA 
GATTTCATAG ATTCATTGTT ACAGGTGGAT CCAAATAATA GGTCGACAGC TGCAAAAGCC 
TTGAATCATC CCTGGATCAA GATGAGTCCA TTGGGCTCAC AATCATATGG TGATTTTTCA 
CAAATATCCT TATCACAATC GTTGTCGCAG CAGAAATTAT TAGAAAATAT GGACGATGCT 
CAATACGAAT TTGTCAAAGC GCAAAGGAAA TTACAAATGG AGCAACAACT TCAAGAACAG 
GATCAGGAAG ACCAAGATGG AAAAATTCAA GGATTTAAAA TACCCGCACA CGCCCCTATT 
CGATATACAC AGCCCAAAAG CATTGAAGCA GAAACTAGAG AACAAAAACT TTTACATTCC 
AATAATACTG AGAATGTCAA GAGCTCAAAG AAAAAGGGTA ATGGTAGGTT TTTAACTTTA 
AAACCATTGC CTGACAGCAT TATTCAAGAA AGCCTGGAGA TTCAGCAAGG TGTGAATCCA 
TTTTTCATTG GTAGATCCGA GGATTGCAAT TGTAAAATTG AAGACAATAG GTTGTCTCGA 
GTTCATTGCT TCATTTTCAA AAAGAGGCAT GCTGTAGGCA AAAGCATGTA TGAATCTCCG 
GCACAAGGTT TAGATGATAT TTGGTATTGC CACACCGGAA CTAACGTGAG CTATTTAAAT 
AATAACCGCA TGATACAGGG TACGAAATTC CTTTTACAAG ACGGAGATGA AATCAAGATC 
ATTTGGGATA AAAACAATAA ATTTGTCATT GGCTTTAAAG TGGAAATTAA CGATACTACA 
GGTCTGTTTA ACGAGGGATT AGGTATGTTA CAAGAACAAA GAGTAGTACT TAAGCAAACA 
GCCGAAGAAA AAGATTTGGT GAAAAAGTTA ACCCAGATGA TGGCAGCTCA ACGTGCAAAT 
CAACCCTCGG CTTCTTCTTC ATCAATGTCG GCTAAGAAGC CGCCAGTTAG CGATACAAAT 
AATAACGGCA ATAATTCGGT ACTAAACGAC TTGGTAGAGT CACCGATTAA TGCGAATACG 
GGGAACATTT TGAAGAGAAT ACATTCGGTA AGTTTATCGC AATCACAAAT TGATCCTAGT 
AAGAAGGTTA AAAGGGCAAA ATTGGACCAA ACCTCAAAAG GCCCCGAGAA TTTGCAATTT 
TCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
Nature387(6632 Suppl)103-5(1997 May)
Bussey H,Storms RK,Ahmed A,Albermann K,Allen E,Ansorge W,Araujo R,Aparicio A,Barrell B,Badcock K,Benes V,Botstein D,Bowman S,Br?ckner M,Carpenter J,Cherry JM,Chung E,Churcher C,Coster F,Davis K,Davis RW,Dietrich FS,Delius H,DiPaolo T,Hani J

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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RAD53 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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