HRR25 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	HRR25
Entrez Gene ID	855897
Full Name	serine/threonine protein kinase HRR25
Synonyms	KTI14
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001184018.1	NP_015120.1	serine/threonine protein kinase HRR25

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	CSNK1D	XP_415634.3
Saccharomyces cerevisiae (baker's yeast)	HRR25	NP_015120.1
Neurospora crassa	NCU00685	XP_965825.2
Xenopus tropicalis (tropical clawed frog)	csnk1d	NP_989089.1
Mus musculus (house mouse)	Csnk1d	NP_620690.1
Rattus norvegicus (Norway rat)	Csnk1d	NP_620691.2
Homo sapiens (human)	CSNK1D	NP_001884.2
Canis lupus familiaris (dog)	CSNK1D	XP_533137.4
Caenorhabditis elegans (roundworm)	kin-20	NP_872247.4
Kluyveromyces lactis	KLLA0D03168g	XP_453206.1
Eremothecium gossypii	AGOS_AFL091W	NP_985457.1
Magnaporthe oryzae (rice blast fungus)	MGG_02829	XP_003720909.1
Pan troglodytes (chimpanzee)	CSNK1D	XP_001167815.1
Danio rerio (zebrafish)	csnk1db	NP_998415.1
Schizosaccharomyces pombe (fission yeast)	hhp1	NP_595760.1
Arabidopsis thaliana (thale cress)	ckl5	NP_179537.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
Bussey H, Storms RK, Ahmed A, Albermann K, Allen E, Ansorge W, Araujo R, Aparicio A, Barrell B, Badcock K, Benes V, Botstein D, Bowman S, Br?ckner M, Carpenter J, Cherry JM, Chung E, Churcher C, Coster F, Davis K, Davis RW, Dietrich FS, Delius H, DiPaolo T, Hani J
Nature387(6632 Suppl)103-5(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The C-terminal region of Hrr25 is required for normal inhibition of expression of Haa1 target genes TPO2 and YRO2 and is important for cell growth but is not required for cell morphogenesis
Title: Casein Kinase I Isoform Hrr25 Is a Negative Regulator of Haa1 in the Weak Acid Stress Response Pathway in Saccharomyces cerevisiae.

Together, the Hrr25 central domain and Mam1 form a novel, contiguous embellishment to the Hrr25 kinase domain that affects Hrr25 conformational dynamics and enzyme kinetics.
Title: Structure of the Saccharomyces cerevisiae Hrr25:Mam1 monopolin subcomplex reveals a novel kinase regulator.

This recruitment requires Hrr25 kinase activity and the Dcp2 decapping enzyme, a core constituent of these RNP granules. Interestingly, the data indicate that this localization sequesters active Hrr25 away from the remainder of the cytoplasm and thereby shields this enzyme from the degradation machinery during these periods of stress.
Title: The Activity-Dependent Regulation of Protein Kinase Stability by the Localization to P-Bodies.

Ypt1 binds and activates Hrr25 to spatially regulate its kinase activity.
Title: Ypt1/Rab1 regulates Hrr25/CK1? kinase activity in ER-Golgi traffic and macroautophagy.

Hrr25 is essential for ribosome assembly in Saccharomyces cerevisiae.
Title: Hrr25/CK1?-directed release of Ltv1 from pre-40S ribosomes is necessary for ribosome assembly and cell growth.

The following HRR25 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HRR25 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04772	NM_001184018.1 Latest version!	Saccharomyces cerevisiae S288C serine/threonine protein kinase HRR25 (HRR25), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HRR25 ( NM_001184018.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_015120.1 Saccharomyces cerevisiae S288C serine/threonine protein kinase HRR25 (HRR25), partial mRNA.

Price & Availability↑

CloneID	OSi04772
Clone ID Related Accession (Same CDS sequence)	NM_001184018.1
Accession Version	NM_001184018.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	serine/threonine protein kinase HRR25
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001148). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGACTTAA GAGTAGGAAG GAAATTTCGT ATTGGCAGGA AGATTGGGAG TGGTTCCTTT 
GGTGACATTT ACCACGGCAC GAACTTAATT AGTGGTGAAG AAGTAGCCAT CAAGCTGGAA 
TCGATCAGGT CCAGACATCC TCAATTGGAC TATGAGTCCC GCGTCTACAG ATACTTAAGC 
GGTGGTGTGG GAATCCCGTT CATCAGATGG TTTGGCAGAG AGGGTGAATA TAATGCTATG 
GTCATCGATC TTCTAGGCCC ATCTTTGGAA GATTTATTCA ACTACTGTCA CAGAAGGTTC 
TCCTTTAAGA CGGTTATCAT GCTGGCTTTG CAAATGTTTT GCCGTATTCA GTATATACAT 
GGAAGGTCGT TCATTCATAG AGATATCAAA CCAGACAACT TTTTAATGGG GGTAGGACGC 
CGTGGTAGCA CCGTTCATGT TATTGATTTC GGTCTATCAA AGAAATACCG AGATTTCAAC 
ACACATCGTC ATATTCCTTA CAGGGAGAAC AAGTCCTTGA CAGGTACAGC TCGTTATGCA 
AGTGTCAATA CGCATCTTGG AATAGAGCAA AGTAGAAGAG ATGACTTAGA ATCACTAGGT 
TATGTCTTGA TCTATTTTTG TAAGGGTTCT TTGCCATGGC AGGGTTTGAA AGCAACCACC 
AAGAAACAAA AGTATGATCG TATCATGGAA AAGAAATTAA ACGTTAGCGT GGAAACTCTA 
TGTTCAGGTT TACCATTAGA GTTTCAAGAA TATATGGCTT ACTGTAAGAA TTTGAAATTC 
GATGAGAAGC CAGATTATTT GTTCTTGGCA AGGCTGTTTA AAGATCTGAG TATTAAACTA 
GAGTATCACA ACGACCACTT GTTCGATTGG ACAATGTTGC GTTACACAAA GGCGATGGTG 
GAGAAGCAAA GGGACCTCCT CATCGAAAAA GGTGATTTGA ACGCAAATAG CAATGCAGCA 
AGTGCAAGTA ACAGCACAGA CAACAAGTCT GAAACTTTCA ACAAGATTAA ACTGTTAGCC 
ATGAAGAAAT TCCCCACCCA TTTCCACTAT TACAAGAATG AAGACAAACA TAATCCTTCA 
CCAGAAGAGA TCAAACAACA AACTATCTTG AATAATAATG CAGCCTCTTC TTTACCAGAG 
GAATTATTGA ACGCACTAGA TAAAGGTATG GAAAACTTGA GACAACAGCA GCCGCAGCAG 
CAGGTCCAAA GTTCGCAGCC ACAACCACAG CCCCAACAGC TACAGCAGCA ACCAAATGGC 
CAAAGACCAA ATTATTATCC TGAACCGTTA CTACAGCAGC AACAAAGAGA TTCTCAGGAG 
CAACAGCAGC AAGTTCCGAT GGCTACAACC AGGGCTACTC AGTATCCCCC ACAAATAAAC 
AGCAATAATT TTAATACTAA TCAAGCATCT GTACCTCCAC AAATGAGATC TAATCCACAA 
CAGCCGCCTC AAGATAAACC AGCTGGCCAG TCAATTTGGT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_015120.1
CDS	1..1485
Translation	MDLRVGRKFRIGRKIGSGSFGDIYHGTNLISGEEVAIKLESIRSRHPQLDYESRVYRYLSGGVGIPFIRWFGREGEYNAMVIDLLGPSLEDLFNYCHRRFSFKTVIMLALQMFCRIQYIHGRSFIHRDIKPDNFLMGVGRRGSTVHVIDFGLSKKYRDFNTHRHIPYRENKSLTGTARYASVNTHLGIEQSRRDDLESLGYVLIYFCKGSLPWQGLKATTKKQKYDRIMEKKLNVSVETLCSGLPLEFQEYMAYCKNLKFDEKPDYLFLARLFKDLSIKLEYHNDHLFDWTMLRYTKAMVEKQRDLLIEKGDLNANSNAASASNSTDNKSETFNKIKLLAMKKFPTHFHYYKNEDKHNPSPEEIKQQTILNNNAASSLPEELLNALDKGMENLRQQQPQQQVQSSQPQPQPQQLQQQPNGQRPNYYPEPLLQQQQRDSQEQQQQVPMATTRATQYPPQINSNNFNTNQASVPPQMRSNPQQPPQDKPAGQSIWL

Target ORF information:

RefSeq Version	NM_001184018.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C serine/threonine protein kinase HRR25 (HRR25), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001184018.1

ORF Insert Sequence:

ATGGACTTAA GAGTAGGAAG GAAATTTCGT ATTGGCAGGA AGATTGGGAG TGGTTCCTTT 
GGTGACATTT ACCACGGCAC GAACTTAATT AGTGGTGAAG AAGTAGCCAT CAAGCTGGAA 
TCGATCAGGT CCAGACATCC TCAATTGGAC TATGAGTCCC GCGTCTACAG ATACTTAAGC 
GGTGGTGTGG GAATCCCGTT CATCAGATGG TTTGGCAGAG AGGGTGAATA TAATGCTATG 
GTCATCGATC TTCTAGGCCC ATCTTTGGAA GATTTATTCA ACTACTGTCA CAGAAGGTTC 
TCCTTTAAGA CGGTTATCAT GCTGGCTTTG CAAATGTTTT GCCGTATTCA GTATATACAT 
GGAAGGTCGT TCATTCATAG AGATATCAAA CCAGACAACT TTTTAATGGG GGTAGGACGC 
CGTGGTAGCA CCGTTCATGT TATTGATTTC GGTCTATCAA AGAAATACCG AGATTTCAAC 
ACACATCGTC ATATTCCTTA CAGGGAGAAC AAGTCCTTGA CAGGTACAGC TCGTTATGCA 
AGTGTCAATA CGCATCTTGG AATAGAGCAA AGTAGAAGAG ATGACTTAGA ATCACTAGGT 
TATGTCTTGA TCTATTTTTG TAAGGGTTCT TTGCCATGGC AGGGTTTGAA AGCAACCACC 
AAGAAACAAA AGTATGATCG TATCATGGAA AAGAAATTAA ACGTTAGCGT GGAAACTCTA 
TGTTCAGGTT TACCATTAGA GTTTCAAGAA TATATGGCTT ACTGTAAGAA TTTGAAATTC 
GATGAGAAGC CAGATTATTT GTTCTTGGCA AGGCTGTTTA AAGATCTGAG TATTAAACTA 
GAGTATCACA ACGACCACTT GTTCGATTGG ACAATGTTGC GTTACACAAA GGCGATGGTG 
GAGAAGCAAA GGGACCTCCT CATCGAAAAA GGTGATTTGA ACGCAAATAG CAATGCAGCA 
AGTGCAAGTA ACAGCACAGA CAACAAGTCT GAAACTTTCA ACAAGATTAA ACTGTTAGCC 
ATGAAGAAAT TCCCCACCCA TTTCCACTAT TACAAGAATG AAGACAAACA TAATCCTTCA 
CCAGAAGAGA TCAAACAACA AACTATCTTG AATAATAATG CAGCCTCTTC TTTACCAGAG 
GAATTATTGA ACGCACTAGA TAAAGGTATG GAAAACTTGA GACAACAGCA GCCGCAGCAG 
CAGGTCCAAA GTTCGCAGCC ACAACCACAG CCCCAACAGC TACAGCAGCA ACCAAATGGC 
CAAAGACCAA ATTATTATCC TGAACCGTTA CTACAGCAGC AACAAAGAGA TTCTCAGGAG 
CAACAGCAGC AAGTTCCGAT GGCTACAACC AGGGCTACTC AGTATCCCCC ACAAATAAAC 
AGCAATAATT TTAATACTAA TCAAGCATCT GTACCTCCAC AAATGAGATC TAATCCACAA 
CAGCCGCCTC AAGATAAACC AGCTGGCCAG TCAATTTGGT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
Nature387(6632 Suppl)103-5(1997 May)
Bussey H,Storms RK,Ahmed A,Albermann K,Allen E,Ansorge W,Araujo R,Aparicio A,Barrell B,Badcock K,Benes V,Botstein D,Bowman S,Br?ckner M,Carpenter J,Cherry JM,Chung E,Churcher C,Coster F,Davis K,Davis RW,Dietrich FS,Delius H,DiPaolo T,Hani J

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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HRR25 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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