GYP5 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	GYP5
Entrez Gene ID	855827
Full Name	Gyp5p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001184063.1	NP_015075.1	Gyp5p

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_AFL161C	NP_985389.2
Saccharomyces cerevisiae (baker's yeast)	GYP5	NP_015075.1
Kluyveromyces lactis	KLLA0D12584g	XP_453623.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
Bussey H, Storms RK, Ahmed A, Albermann K, Allen E, Ansorge W, Araujo R, Aparicio A, Barrell B, Badcock K, Benes V, Botstein D, Bowman S, Br?ckner M, Carpenter J, Cherry JM, Chung E, Churcher C, Coster F, Davis K, Davis RW, Dietrich FS, Delius H, DiPaolo T, Hani J
Nature387(6632 Suppl)103-5(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In gyp5Deltagyl1Delta cells, Rvs167p is not correctly localized to the small-bud tip.
Title: The RabGAP proteins Gyp5p and Gyl1p recruit the BAR domain protein Rvs167p for polarized exocytosis.

Results describe the interaction between Gyp5p and Gyl1p as well as the mechanism of their localization to the sites of polarized growth, and show that purified recombinant Gyp5p and Gyl1p interact directly in vitro.
Title: Interdependence of the Ypt/RabGAP Gyp5p and Gyl1p for recruitment to the sites of polarized growth.

These data suggest that Gyp5p and Gyl1p are involved in control of polarized exocytosis.
Title: Gyp5p and Gyl1p are involved in the control of polarized exocytosis in budding yeast.

The following GYP5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GYP5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04709	NM_001184063.1 Latest version!	Saccharomyces cerevisiae S288C Gyp5p (GYP5), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GYP5 ( NM_001184063.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_015075.1 Saccharomyces cerevisiae S288C Gyp5p (GYP5), partial mRNA.

Price & Availability↑

CloneID	OSi04709
Clone ID Related Accession (Same CDS sequence)	NM_001184063.1
Accession Version	NM_001184063.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Gyp5p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001148). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCTTCAG ACAAATCTAT TGAGAAAAAT ACAGATACGA TCGCCTCTGA AGTTCACGAA 
GGTGATAATC ATTCGAATAA TTTGGGTTCA ATGGAGGAAG AGATAAAATC AACGCCATCA 
GACCAATATG AAGAGATAGC TATAATTCCA ACTGAGCCCC TCCATTCGGA CAAAGAACTA 
AATGACAAGC AACAAAGTTT AGGCCATGAA GCACCCACAA ATGTATCAAG AGAAGAACCT 
ATTGGGATCT CTGGTGATGA AGACACCCAA ATCACTGAAC AAAACGTTAA TGAACAAAGG 
CAAGAAACGA GAGAACCTAG TTCTGAAATT GATTTAAATG AGCCATTGGA TGTTGAAAAA 
GATGTGACAA CGGATGTGCA AGCCCCAAAT GGGCTGAATA TTGAAAAAGA ATATGATGCA 
GTCAAAGAGA ATGAGAAGGT TTACGCTGAT ACGAAAGAGG TTGTTAGTAG TCCAGAAAAT 
AGGGAAGTAA CTGGAAAAAA TTCTGGAGGT GAGAAATCAT CCTCGAGTAA ATTTCTCGAT 
GACGAATCTG GTACTACGAC AGCTGCAAAT GCGAACGACA TTTCTATAAG TAGTGAAGTA 
ACCCCAGAAA GGTCGTCAGA AAATGATAAT AACCAGATTC ATATTACCAA TGAGGTAGCC 
GCAGGAATAA ACTTGAATGA AAACAAGGAA CAAAAAGCTG CTATAGAAGA TGGGCCCGTA 
ACAGCAGAGA ATCTTTCCTC AGAAACTGCT AGGAAAGTAC CACCTATACC CACACAAATC 
ATAAATGAAA AAGGTGATAA CTCAAGCGAG AACGAGGTTT CCGCAATTCC CACTACATCT 
TCGCCACCAT TACCACCTAG ACAAAATGTT GCGACATCAA CGTCACCAAA ATTACCTCCA 
AGGGGCAAAC AAAGGGAACA ACCACCGAAG ACGAAGAATG CTGTTCCACC GCCACTAGAG 
GAAGAAATGA AGTCAGAGAA GTTCCGTAAA AACTTCGAAG AAACTAAAAG AAATAGTTAT 
CATCATGTTC CTCTCACTGG TTCCAAAACT GCGCAATTAG AGTCCACTGC AGAAATTAAT 
TTGATCGCTA GTCGTTACCG CAAGACCAGT CACCATTTGA ATAAAGAGGG GGAAGAAACT 
AGAGAGTCGT TGCAGGAAGG TCAAAGCTTT TTAAAATCGA CATTTACGTC ATTTCTGGAG 
AACTTGTCCG AGTATAACGA AGTGGAGAAT GTTAATGAAG AAGATAGAGA AATGTTTAAA 
ATTGATTGGT CCTTCTGGAC TCAAGTAGTT AATGATTATG CTACAGTGGC AAGCAACGAA 
CCAGAAAATT TAGAAGCCCA TGTTACCAAT GGGATACCAC CACAAATTCG AGGTATCATT 
TGGCAGCTGA TGGCGAATTC CAAATCAAGA GAAATGGAAG ATATATATGA AACTCTATTG 
GATACCGAAT GTCTTCATGA AGCAACAATA CGTAGAGATT TAAGGAGAAC GAAGTTTGTG 
GCCGAGGACA AAATGGAGTC ACTATACAAG GTAATAAAGG TGTATTCTGT TTATGATCCA 
GATGTTGGGT ACACGCAAGG CATGGGGTTC ATTGCAGCGC CGCTATTAAT AAACTGTGAA 
AACGAGGCCG AATCTTTTGG ACTACTCGTT GGATTAATGA AGAATTATGG ATTAAGAGAG 
CTTTTCTTAC CAGGAATGCC TGGGCTGATG TTAATGCTTT ATCAATTTGA TAGATTATTA 
GAGGAGCATT CACCTTCTTT GTACAATCGC TTGATCCGTG AGGGAATTAG CTCCACAATG 
TACGCTACAC AATGGTTTTT GACATTTTTC GCATACAAGT TTCCGTTAGA GTTTGTGTTA 
AGAATTTTTG ATATTGTATT CGTAGAGGGT ATTGAAGTGC TTTTGAAGTT TGCTGTAAAT 
TTAATGTTAA AGAATGAAGA AACTTTAGTA AAATTGAGAT TCGATGAACT ATTGGATTTT 
TTAAAGGACG AACTCTTCAA TTACTATTTA ATGGGAAACC AGGATGACGC TTCTGTAGTG 
CAAATGGGAT TGTCAAATGG GAACTCATTT AAGGGCAATG ATGACGGCAC TTTTTCCTAC 
AATGTCGATC TTTTCGTTCA TGATGCAATG ACAGGTGTAT ATATCACACC ATTAACATTA 
AGAAGATACA GGGGAGAGTA CGTAGAAATC CATGAAAAAG AGCAAAAAAA AGAGGACCAC 
TACGAATCAC TAAGAATTCA AAACCACCAA CTACAAAGAG AGGCGCAGAA GCTAGAACAC 
GACTATTCAA TTTTGAACAA AGAGAATATC AGCGCCGCTA ACGAGCTGAT TCAGAACAGA 
TTGAATATGG AGATGCTGTT GGATGAAAAA AACGACCTGA TAAATACAAT CACTGATATC 
AAAAGCCAAA TTGAGGAAGA GATTCGCAAG CAAAATTTAC CGAACCCGGA CGCGTCACTA 
CCAAAGGCAG ATCTGAGAGA AGACTTGGAA AGGACCATAT CCAGGAACAA TGAGGTGATG 
AGGGAGAACG GCCAATTAGA GGAAAGAATC ACTGAACTGC AAGCGGAAAT CGATGAGTTG 
ATTAACATCA ATAAGGAGCA AGTGTCCACG GCATCGTTAT TGGAACGAGA TTCTAAGGCT 
AAAGGGAGGA AAGGCTGGAC TGGTTTTAAA AAAGTTTTTA AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_015075.1
CDS	1..2685
Translation	MSSDKSIEKNTDTIASEVHEGDNHSNNLGSMEEEIKSTPSDQYEEIAIIPTEPLHSDKELNDKQQSLGHEAPTNVSREEPIGISGDEDTQITEQNVNEQRQETREPSSEIDLNEPLDVEKDVTTDVQAPNGLNIEKEYDAVKENEKVYADTKEVVSSPENREVTGKNSGGEKSSSSKFLDDESGTTTAANANDISISSEVTPERSSENDNNQIHITNEVAAGINLNENKEQKAAIEDGPVTAENLSSETARKVPPIPTQIINEKGDNSSENEVSAIPTTSSPPLPPRQNVATSTSPKLPPRGKQREQPPKTKNAVPPPLEEEMKSEKFRKNFEETKRNSYHHVPLTGSKTAQLESTAEINLIASRYRKTSHHLNKEGEETRESLQEGQSFLKSTFTSFLENLSEYNEVENVNEEDREMFKIDWSFWTQVVNDYATVASNEPENLEAHVTNGIPPQIRGIIWQLMANSKSREMEDIYETLLDTECLHEATIRRDLRRTKFVAEDKMESLYKVIKVYSVYDPDVGYTQGMGFIAAPLLINCENEAESFGLLVGLMKNYGLRELFLPGMPGLMLMLYQFDRLLEEHSPSLYNRLIREGISSTMYATQWFLTFFAYKFPLEFVLRIFDIVFVEGIEVLLKFAVNLMLKNEETLVKLRFDELLDFLKDELFNYYLMGNQDDASVVQMGLSNGNSFKGNDDGTFSYNVDLFVHDAMTGVYITPLTLRRYRGEYVEIHEKEQKKEDHYESLRIQNHQLQREAQKLEHDYSILNKENISAANELIQNRLNMEMLLDEKNDLINTITDIKSQIEEEIRKQNLPNPDASLPKADLREDLERTISRNNEVMRENGQLEERITELQAEIDELININKEQVSTASLLERDSKAKGRKGWTGFKKVFK

Target ORF information:

RefSeq Version	NM_001184063.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Gyp5p (GYP5), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001184063.1

ORF Insert Sequence:

ATGTCTTCAG ACAAATCTAT TGAGAAAAAT ACAGATACGA TCGCCTCTGA AGTTCACGAA 
GGTGATAATC ATTCGAATAA TTTGGGTTCA ATGGAGGAAG AGATAAAATC AACGCCATCA 
GACCAATATG AAGAGATAGC TATAATTCCA ACTGAGCCCC TCCATTCGGA CAAAGAACTA 
AATGACAAGC AACAAAGTTT AGGCCATGAA GCACCCACAA ATGTATCAAG AGAAGAACCT 
ATTGGGATCT CTGGTGATGA AGACACCCAA ATCACTGAAC AAAACGTTAA TGAACAAAGG 
CAAGAAACGA GAGAACCTAG TTCTGAAATT GATTTAAATG AGCCATTGGA TGTTGAAAAA 
GATGTGACAA CGGATGTGCA AGCCCCAAAT GGGCTGAATA TTGAAAAAGA ATATGATGCA 
GTCAAAGAGA ATGAGAAGGT TTACGCTGAT ACGAAAGAGG TTGTTAGTAG TCCAGAAAAT 
AGGGAAGTAA CTGGAAAAAA TTCTGGAGGT GAGAAATCAT CCTCGAGTAA ATTTCTCGAT 
GACGAATCTG GTACTACGAC AGCTGCAAAT GCGAACGACA TTTCTATAAG TAGTGAAGTA 
ACCCCAGAAA GGTCGTCAGA AAATGATAAT AACCAGATTC ATATTACCAA TGAGGTAGCC 
GCAGGAATAA ACTTGAATGA AAACAAGGAA CAAAAAGCTG CTATAGAAGA TGGGCCCGTA 
ACAGCAGAGA ATCTTTCCTC AGAAACTGCT AGGAAAGTAC CACCTATACC CACACAAATC 
ATAAATGAAA AAGGTGATAA CTCAAGCGAG AACGAGGTTT CCGCAATTCC CACTACATCT 
TCGCCACCAT TACCACCTAG ACAAAATGTT GCGACATCAA CGTCACCAAA ATTACCTCCA 
AGGGGCAAAC AAAGGGAACA ACCACCGAAG ACGAAGAATG CTGTTCCACC GCCACTAGAG 
GAAGAAATGA AGTCAGAGAA GTTCCGTAAA AACTTCGAAG AAACTAAAAG AAATAGTTAT 
CATCATGTTC CTCTCACTGG TTCCAAAACT GCGCAATTAG AGTCCACTGC AGAAATTAAT 
TTGATCGCTA GTCGTTACCG CAAGACCAGT CACCATTTGA ATAAAGAGGG GGAAGAAACT 
AGAGAGTCGT TGCAGGAAGG TCAAAGCTTT TTAAAATCGA CATTTACGTC ATTTCTGGAG 
AACTTGTCCG AGTATAACGA AGTGGAGAAT GTTAATGAAG AAGATAGAGA AATGTTTAAA 
ATTGATTGGT CCTTCTGGAC TCAAGTAGTT AATGATTATG CTACAGTGGC AAGCAACGAA 
CCAGAAAATT TAGAAGCCCA TGTTACCAAT GGGATACCAC CACAAATTCG AGGTATCATT 
TGGCAGCTGA TGGCGAATTC CAAATCAAGA GAAATGGAAG ATATATATGA AACTCTATTG 
GATACCGAAT GTCTTCATGA AGCAACAATA CGTAGAGATT TAAGGAGAAC GAAGTTTGTG 
GCCGAGGACA AAATGGAGTC ACTATACAAG GTAATAAAGG TGTATTCTGT TTATGATCCA 
GATGTTGGGT ACACGCAAGG CATGGGGTTC ATTGCAGCGC CGCTATTAAT AAACTGTGAA 
AACGAGGCCG AATCTTTTGG ACTACTCGTT GGATTAATGA AGAATTATGG ATTAAGAGAG 
CTTTTCTTAC CAGGAATGCC TGGGCTGATG TTAATGCTTT ATCAATTTGA TAGATTATTA 
GAGGAGCATT CACCTTCTTT GTACAATCGC TTGATCCGTG AGGGAATTAG CTCCACAATG 
TACGCTACAC AATGGTTTTT GACATTTTTC GCATACAAGT TTCCGTTAGA GTTTGTGTTA 
AGAATTTTTG ATATTGTATT CGTAGAGGGT ATTGAAGTGC TTTTGAAGTT TGCTGTAAAT 
TTAATGTTAA AGAATGAAGA AACTTTAGTA AAATTGAGAT TCGATGAACT ATTGGATTTT 
TTAAAGGACG AACTCTTCAA TTACTATTTA ATGGGAAACC AGGATGACGC TTCTGTAGTG 
CAAATGGGAT TGTCAAATGG GAACTCATTT AAGGGCAATG ATGACGGCAC TTTTTCCTAC 
AATGTCGATC TTTTCGTTCA TGATGCAATG ACAGGTGTAT ATATCACACC ATTAACATTA 
AGAAGATACA GGGGAGAGTA CGTAGAAATC CATGAAAAAG AGCAAAAAAA AGAGGACCAC 
TACGAATCAC TAAGAATTCA AAACCACCAA CTACAAAGAG AGGCGCAGAA GCTAGAACAC 
GACTATTCAA TTTTGAACAA AGAGAATATC AGCGCCGCTA ACGAGCTGAT TCAGAACAGA 
TTGAATATGG AGATGCTGTT GGATGAAAAA AACGACCTGA TAAATACAAT CACTGATATC 
AAAAGCCAAA TTGAGGAAGA GATTCGCAAG CAAAATTTAC CGAACCCGGA CGCGTCACTA 
CCAAAGGCAG ATCTGAGAGA AGACTTGGAA AGGACCATAT CCAGGAACAA TGAGGTGATG 
AGGGAGAACG GCCAATTAGA GGAAAGAATC ACTGAACTGC AAGCGGAAAT CGATGAGTTG 
ATTAACATCA ATAAGGAGCA AGTGTCCACG GCATCGTTAT TGGAACGAGA TTCTAAGGCT 
AAAGGGAGGA AAGGCTGGAC TGGTTTTAAA AAAGTTTTTA AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
Nature387(6632 Suppl)103-5(1997 May)
Bussey H,Storms RK,Ahmed A,Albermann K,Allen E,Ansorge W,Araujo R,Aparicio A,Barrell B,Badcock K,Benes V,Botstein D,Bowman S,Br?ckner M,Carpenter J,Cherry JM,Chung E,Churcher C,Coster F,Davis K,Davis RW,Dietrich FS,Delius H,DiPaolo T,Hani J

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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GYP5 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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