BIO3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	BIO3
Entrez Gene ID	855795
Full Name	adenosylmethionine-8-amino-7-oxononanoate transaminase
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001183235.1	NP_014456.1	adenosylmethionine-8-amino-7-oxononanoate transaminase

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	BIO1	NP_200567.2
Saccharomyces cerevisiae (baker's yeast)	BIO3	NP_014456.1
Oryza sativa (rice)	Os08g0245400	NP_001061354.1
Kluyveromyces lactis	KLLA0B00429g	XP_451549.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Philippsen P, Kleine K, P?hlmann R, D?sterh?ft A, Hamberg K, Hegemann JH, Obermaier B, Urrestarazu LA, Aert R, Albermann K, Altmann R, Andr? B, Baladron V, Ballesta JP, B?cam AM, Beinhauer J, Boskovic J, Buitrago MJ, Bussereau F, Coster F, Crouzet M, D'Angelo M, Dal Pero F, De Antoni A, Del Rey F, Doignon F, Domdey H, Dubois E, Fiedler T, Fleig U, Floeth M, Fritz C, Gaillardin C, Garcia-Cantalejo JM, Glansdorff NN, Goffeau A, Gueldener U, Herbert C, Heumann K, Heuss-Neitzel D, Hilbert H, Hinni K, Iraqui Houssaini I, Jacquet M, Jimenez A, Jonniaux JL, Karpfinger L, Lanfranchi G, Lepingle A, Levesque H, Lyck R, Maftahi M, Mallet L, Maurer KC, Messenguy F, Mewes HW, M?sti D, Nasr F, Nicaud JM, Niedenthal RK, Pandolfo D, Pi?rard A, Piravandi E, Planta RJ, Pohl TM, Purnelle B, Rebischung C, Remacha M, Revuelta JL, Rinke M, Saiz JE, Sartorello F, Scherens B, Sen-Gupta M, Soler-Mira A, Urbanus JH, Valle G, Van Dyck L, Verhasselt P, Vierendeels F, Vissers S, Voet M, Volckaert G, Wach A, Wambutt R, Wedler H, Zollner A, Hani J
Nature387(6632 Suppl)93-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Quantitative trait loci analysis suggest that polymorphism in the coding region of GCN1, MDS3, ARG81 and BIO3 could account for differences in nitrogen requirement.
Title: A genetic approach of wine yeast fermentation capacity in nitrogen-starvation reveals the key role of nitrogen signaling.

The following BIO3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BIO3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04682	NM_001183235.1 Latest version!	Saccharomyces cerevisiae S288C adenosylmethionine-8-amino-7-oxononanoate transaminase (BIO3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BIO3 ( NM_001183235.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_014456.1 Saccharomyces cerevisiae S288C adenosylmethionine-8-amino-7-oxononanoate transaminase (BIO3), partial mRNA.

Price & Availability↑

CloneID	OSi04682
Clone ID Related Accession (Same CDS sequence)	NM_001183235.1
Accession Version	NM_001183235.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1443bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	adenosylmethionine-8-amino-7-oxononanoate transaminase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001146). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCCCAAG AAATTTCATA TACACCAGAT GTCGCGGAAC TGCTAGACTT TGATAAAAAA 
CATATTTGGC ATCCTTATAC GTCATTGAGT TCACCATTGA ACGTTTATCC GGTAAAGAGT 
GCTCACGGAT GCAAGCTGGT GTTGGATACC GACTCACCAG TGGACGTCGA GGTGATCGAC 
GCCATGTCGT CGTGGTGGTG TGTTATTCAC GGGTACAATA ATCCAGAACT AAATGAGGCC 
CTTACCAAGC AGATGTTAAA GTTTTCTCAC GTCCTTCTTG GTGGATTCAC CCATAAAGGA 
GCTGTGAATC TAGTCCAAAA ACTTTTAAAA GTGATTGATG AACCCTCTTT GCAGTACTGT 
TTCCTCGCAG ATTCTGGCTC AGTAGCAGTG GAAGTAGCTT TGAAGATGGC TTTACAATCA 
AACATGTCAG GGGAAGCTAC TAAAAATAGA ACAAAGTTTT TGACAATCAA GAACGGGTAT 
CATGGTGATA CATTTGGCGC TATGAGTGTT TGTGATCCTG AGAATTCTAT GCATCATATC 
TACAACGATC GACTCAGTGA GAACATCTTC GCCCAAGCTC CCTCTATTGT CGATGGCCTT 
CCAACCAGCC AGAACGGTTT CGAGGACCAT TGGAATGCAG AAGAAGTTAC CGATCTCAAG 
AAGCAATTTG AATTGCATAG TGATAAGATC TGTGCTGTGA TTTTGGAGCC TATCTTACAG 
GGTGCAGGAG GTTTGCGACC ATACCATCCA CAGTTCTTAA TCGAAGTTCA GAAACTATGT 
AACCAATACG ACGTTTTATT CATCATGGAC GAGATTGCTA CAGGATTTGG TAGAACAGGT 
GAGATTTTCG CATTCAAGCA CTGTCAAAAA TACCAGGACC AACATGGCAT CAGTCCTTCC 
GATCAGATCA AAGTTGTTCC TGACATATTG TGCGTTGGGA AGGGCTTAAC CAGTGGATAT 
ATGACTATGA GTGCAGTGGT AGTCAACGAC AAAGTTGCTT CCAGAATCTC TTCTCCAAAT 
AGCCCTACTG GTGGCTGCTT CATGCATGGC CCCACCTTCA TGGGCAATGC CCTGGCATGC 
AGTGTGGCAG AAAAGTCTAT GGATATACTA CTGCGTGGCG AGTGGAGAAA ACAAGTGTCT 
GCAATTGAAA ACCAAATTTA TAGAGAACTA TACCAGTACA TCAAGAACCC AGATAATGGG 
CTCATTGGAA CTGTTGTTAA GCGTGTGTCA GTTATTGGAG CTGTAGGTAT CGTAGAGCTA 
TACAAGAAGA CAGATCCCGA GTGGTTCCAG AAAAAGTTTA TATCCAAGGG AGTACATATC 
CGACCTTTCA ACTGCCTGTG TTACATTATG CCACCATATG TGATCACGAC AGAGGAATTG 
ACGAAGGTTA ATCAAGTCTT GATTGAAGTA TTACATGAGT GGAAATCCCA CATAAACCAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_014456.1
CDS	1..1443
Translation	MSQEISYTPDVAELLDFDKKHIWHPYTSLSSPLNVYPVKSAHGCKLVLDTDSPVDVEVIDAMSSWWCVIHGYNNPELNEALTKQMLKFSHVLLGGFTHKGAVNLVQKLLKVIDEPSLQYCFLADSGSVAVEVALKMALQSNMSGEATKNRTKFLTIKNGYHGDTFGAMSVCDPENSMHHIYNDRLSENIFAQAPSIVDGLPTSQNGFEDHWNAEEVTDLKKQFELHSDKICAVILEPILQGAGGLRPYHPQFLIEVQKLCNQYDVLFIMDEIATGFGRTGEIFAFKHCQKYQDQHGISPSDQIKVVPDILCVGKGLTSGYMTMSAVVVNDKVASRISSPNSPTGGCFMHGPTFMGNALACSVAEKSMDILLRGEWRKQVSAIENQIYRELYQYIKNPDNGLIGTVVKRVSVIGAVGIVELYKKTDPEWFQKKFISKGVHIRPFNCLCYIMPPYVITTEELTKVNQVLIEVLHEWKSHINQ

Target ORF information:

RefSeq Version	NM_001183235.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C adenosylmethionine-8-amino-7-oxononanoate transaminase (BIO3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001183235.1

ORF Insert Sequence:

ATGTCCCAAG AAATTTCATA TACACCAGAT GTCGCGGAAC TGCTAGACTT TGATAAAAAA 
CATATTTGGC ATCCTTATAC GTCATTGAGT TCACCATTGA ACGTTTATCC GGTAAAGAGT 
GCTCACGGAT GCAAGCTGGT GTTGGATACC GACTCACCAG TGGACGTCGA GGTGATCGAC 
GCCATGTCGT CGTGGTGGTG TGTTATTCAC GGGTACAATA ATCCAGAACT AAATGAGGCC 
CTTACCAAGC AGATGTTAAA GTTTTCTCAC GTCCTTCTTG GTGGATTCAC CCATAAAGGA 
GCTGTGAATC TAGTCCAAAA ACTTTTAAAA GTGATTGATG AACCCTCTTT GCAGTACTGT 
TTCCTCGCAG ATTCTGGCTC AGTAGCAGTG GAAGTAGCTT TGAAGATGGC TTTACAATCA 
AACATGTCAG GGGAAGCTAC TAAAAATAGA ACAAAGTTTT TGACAATCAA GAACGGGTAT 
CATGGTGATA CATTTGGCGC TATGAGTGTT TGTGATCCTG AGAATTCTAT GCATCATATC 
TACAACGATC GACTCAGTGA GAACATCTTC GCCCAAGCTC CCTCTATTGT CGATGGCCTT 
CCAACCAGCC AGAACGGTTT CGAGGACCAT TGGAATGCAG AAGAAGTTAC CGATCTCAAG 
AAGCAATTTG AATTGCATAG TGATAAGATC TGTGCTGTGA TTTTGGAGCC TATCTTACAG 
GGTGCAGGAG GTTTGCGACC ATACCATCCA CAGTTCTTAA TCGAAGTTCA GAAACTATGT 
AACCAATACG ACGTTTTATT CATCATGGAC GAGATTGCTA CAGGATTTGG TAGAACAGGT 
GAGATTTTCG CATTCAAGCA CTGTCAAAAA TACCAGGACC AACATGGCAT CAGTCCTTCC 
GATCAGATCA AAGTTGTTCC TGACATATTG TGCGTTGGGA AGGGCTTAAC CAGTGGATAT 
ATGACTATGA GTGCAGTGGT AGTCAACGAC AAAGTTGCTT CCAGAATCTC TTCTCCAAAT 
AGCCCTACTG GTGGCTGCTT CATGCATGGC CCCACCTTCA TGGGCAATGC CCTGGCATGC 
AGTGTGGCAG AAAAGTCTAT GGATATACTA CTGCGTGGCG AGTGGAGAAA ACAAGTGTCT 
GCAATTGAAA ACCAAATTTA TAGAGAACTA TACCAGTACA TCAAGAACCC AGATAATGGG 
CTCATTGGAA CTGTTGTTAA GCGTGTGTCA GTTATTGGAG CTGTAGGTAT CGTAGAGCTA 
TACAAGAAGA CAGATCCCGA GTGGTTCCAG AAAAAGTTTA TATCCAAGGG AGTACATATC 
CGACCTTTCA ACTGCCTGTG TTACATTATG CCACCATATG TGATCACGAC AGAGGAATTG 
ACGAAGGTTA ATCAAGTCTT GATTGAAGTA TTACATGAGT GGAAATCCCA CATAAACCAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Nature387(6632 Suppl)93-8(1997 May)
Philippsen P,Kleine K,P?hlmann R,D?sterh?ft A,Hamberg K,Hegemann JH,Obermaier B,Urrestarazu LA,Aert R,Albermann K,Altmann R,Andr? B,Baladron V,Ballesta JP,B?cam AM,Beinhauer J,Boskovic J,Buitrago MJ,Bussereau F,Coster F,Crouzet M,D'Angelo M,Dal Pero F,De Antoni A,Del Rey F,Doignon F,Domdey H,Dubois E,Fiedler T,Fleig U,Floeth M,Fritz C,Gaillardin C,Garcia-Cantalejo JM,Glansdorff NN,Goffeau A,Gueldener U,Herbert C,Heumann K,Heuss-Neitzel D,Hilbert H,Hinni K,Iraqui Houssaini I,Jacquet M,Jimenez A,Jonniaux JL,Karpfinger L,Lanfranchi G,Lepingle A,Levesque H,Lyck R,Maftahi M,Mallet L,Maurer KC,Messenguy F,Mewes HW,M?sti D,Nasr F,Nicaud JM,Niedenthal RK,Pandolfo D,Pi?rard A,Piravandi E,Planta RJ,Pohl TM,Purnelle B,Rebischung C,Remacha M,Revuelta JL,Rinke M,Saiz JE,Sartorello F,Scherens B,Sen-Gupta M,Soler-Mira A,Urbanus JH,Valle G,Van Dyck L,Verhasselt P,Vierendeels F,Vissers S,Voet M,Volckaert G,Wach A,Wambutt R,Wedler H,Zollner A,Hani J

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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BIO3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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