BRE5 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	BRE5
Entrez Gene ID	855787
Full Name	Bre5p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001183228.1	NP_014449.1	Bre5p

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0C18799g	XP_453038.1
Saccharomyces cerevisiae (baker's yeast)	BRE5	NP_014449.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Philippsen P, Kleine K, P?hlmann R, D?sterh?ft A, Hamberg K, Hegemann JH, Obermaier B, Urrestarazu LA, Aert R, Albermann K, Altmann R, Andr? B, Baladron V, Ballesta JP, B?cam AM, Beinhauer J, Boskovic J, Buitrago MJ, Bussereau F, Coster F, Crouzet M, D'Angelo M, Dal Pero F, De Antoni A, Del Rey F, Doignon F, Domdey H, Dubois E, Fiedler T, Fleig U, Floeth M, Fritz C, Gaillardin C, Garcia-Cantalejo JM, Glansdorff NN, Goffeau A, Gueldener U, Herbert C, Heumann K, Heuss-Neitzel D, Hilbert H, Hinni K, Iraqui Houssaini I, Jacquet M, Jimenez A, Jonniaux JL, Karpfinger L, Lanfranchi G, Lepingle A, Levesque H, Lyck R, Maftahi M, Mallet L, Maurer KC, Messenguy F, Mewes HW, M?sti D, Nasr F, Nicaud JM, Niedenthal RK, Pandolfo D, Pi?rard A, Piravandi E, Planta RJ, Pohl TM, Purnelle B, Rebischung C, Remacha M, Revuelta JL, Rinke M, Saiz JE, Sartorello F, Scherens B, Sen-Gupta M, Soler-Mira A, Urbanus JH, Valle G, Van Dyck L, Verhasselt P, Vierendeels F, Vissers S, Voet M, Volckaert G, Wach A, Wambutt R, Wedler H, Zollner A, Hani J
Nature387(6632 Suppl)93-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

show that mature ribosomes are rapidly degraded by autophagy upon nutrient starvation in Saccharomyces cerevisiae, and that selective degradation of ribosomes requires catalytic activity of the Ubp3p/Bre5p ubiquitin protease.
Title: Mature ribosomes are selectively degraded upon starvation by an autophagy pathway requiring the Ubp3p/Bre5p ubiquitin protease.

Ubp3/Bre5 functions antagonistically with Bul1 in DNA damage responses, but not in its other cellular functions
Title: The Bre5/Ubp3 ubiquitin protease complex from budding yeast contributes to the cellular response to DNA damage.

New insights into Ubp3 recognition by Bre5 and into protein recognition by NTF2-like domains.
Title: Molecular basis for bre5 cofactor recognition by the ubp3 deubiquitylating enzyme.

analysis of Ubp3 deubiquitinating enzyme interaction with its Bre5 cofactor
Title: Structural basis for interaction between the Ubp3 deubiquitinating enzyme and its Bre5 cofactor.

The following BRE5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRE5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04675	NM_001183228.1 Latest version!	Saccharomyces cerevisiae S288C Bre5p (BRE5), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BRE5 ( NM_001183228.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_014449.1 Saccharomyces cerevisiae S288C Bre5p (BRE5), partial mRNA.

Price & Availability↑

CloneID	OSi04675
Clone ID Related Accession (Same CDS sequence)	NM_001183228.1
Accession Version	NM_001183228.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1548bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Bre5p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001146). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGGTGTTA CCGTTCAAGA CATATGTTTT GCCTTTTTGC AAAACTACTA CGAAAGAATG 
AGGACCGACC CTTCCAAATT GGCATATTTT TATGCAAGTA CAGCCGAGTT GACGCATACT 
AATTATCAGT CCAAGTCAAC AAACGAAAAA GATGACGTTC TTCCAACGGT GAAAGTTACT 
GGACGTGAAA ACATTAATAA GTTTTTTTCT AGAAATGATG CCAAAGTTAG GAGCTTGAAA 
CTAAAATTAG ATACTATAGA TTTCCAGTAC ACTGGGCACT TGCACAAAAG CATTCTGATC 
ATGGCCACGG GTGAAATGTT TTGGACAGGA ACTCCGGTCT ATAAGTTTTG TCAAACATTT 
ATCCTGCTAC CATCCAGCAA TGGGTCTACG TTTGACATTA CCAATGATAT AATCAGATTT 
ATTTCCAATT CATTCAAGCC TTATGTCTTA ACAGATGCTT CTTTGTCACA ATCTAACGAG 
GAGAACTCTG TCAGTGCAGT TGAAGAAGAT AAAATAAGGC ATGAGTCCGG AGTTGAAAAA 
GAAAAAGAAA AAGAAAAATC ACCAGAAATA TCGAAACCAA AGGCCAAAAA GGAAACGGTC 
AAAGACACAA CTGCGCCAAC TGAATCGTCA ACTCAGGAAA AACCAATTGT CGATCATAGT 
CAACCTCGGG CTATACCCGT CACTAAAGAG TCCAAGATTC ACACCGAAAC GGTACCATCT 
AGTACTAAGG GCAACCACAA ACAAGATGAG GTCTCTACAG AAGAGTTAGG AAACGTTACT 
AAACTAAATG AAAAATCTCA CAAAGCCGAA AAGAAAGCTG CACCAATCAA GACAAAGGAA 
GGCTCTGTTG AGGCCATAAA TGCTGTTAAT AACTCTTCTT TGCCCAACGG TAAAGAAGTT 
AGTGATGAAA AACCGGTACC TGGGGGCGTA AAGGAAGCTG AAACTGAAAT TAAACCAATA 
GAGCCACAGG TTTCCGACGC CAAAGAATCC GGCAATAACG CTTCAACACC TTCATCTTCT 
CCAGAGCCCG TTGCGAATCC ACCAAAGATG ACATGGGCCT CAAAGTTGAT GAATGAAAAC 
TCCGACAGAA TTAGCAAAAA TAATACCACG GTTGAATACA TCAGACCTGA AACACTACCC 
AAGAAACCGA CTGAAAGAAA ATTTGAAATG GGGAATCGGA GGGACAACGC TAGTGCTAAC 
AGCAAAAATA AGAAAAAACC AGTATTCAGC ACCGTTAACA AGGACGGCTT TTATCCAATC 
TACATTAGAG GTACAAATGG CTTAAGAGAA GAAAAATTGA GAAGTGCCTT AGAAAAAGAA 
TTCGGTAAAG TTATGAGGAT AACAGCTGCA GACAATTTTG CCGTCGTCGA CTTTGAAACT 
CAAAAGAGCC AAATCGATGC TTTAGAGAAG AAGAAAAAGT CAATTGATGG TATTGAAGTA 
TGCTTAGAAA GAAAAACAGT AAAGAAGCCC ACGAGCAATA ATCCTCCTGG CATATTTACT 
AATGGAACAC GTTCTCATAG AAAGCAACCC CTAAAAAGAA AGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_014449.1
CDS	1..1548
Translation	MGVTVQDICFAFLQNYYERMRTDPSKLAYFYASTAELTHTNYQSKSTNEKDDVLPTVKVTGRENINKFFSRNDAKVRSLKLKLDTIDFQYTGHLHKSILIMATGEMFWTGTPVYKFCQTFILLPSSNGSTFDITNDIIRFISNSFKPYVLTDASLSQSNEENSVSAVEEDKIRHESGVEKEKEKEKSPEISKPKAKKETVKDTTAPTESSTQEKPIVDHSQPRAIPVTKESKIHTETVPSSTKGNHKQDEVSTEELGNVTKLNEKSHKAEKKAAPIKTKEGSVEAINAVNNSSLPNGKEVSDEKPVPGGVKEAETEIKPIEPQVSDAKESGNNASTPSSSPEPVANPPKMTWASKLMNENSDRISKNNTTVEYIRPETLPKKPTERKFEMGNRRDNASANSKNKKKPVFSTVNKDGFYPIYIRGTNGLREEKLRSALEKEFGKVMRITAADNFAVVDFETQKSQIDALEKKKKSIDGIEVCLERKTVKKPTSNNPPGIFTNGTRSHRKQPLKRKD

Target ORF information:

RefSeq Version	NM_001183228.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Bre5p (BRE5), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001183228.1

ORF Insert Sequence:

ATGGGTGTTA CCGTTCAAGA CATATGTTTT GCCTTTTTGC AAAACTACTA CGAAAGAATG 
AGGACCGACC CTTCCAAATT GGCATATTTT TATGCAAGTA CAGCCGAGTT GACGCATACT 
AATTATCAGT CCAAGTCAAC AAACGAAAAA GATGACGTTC TTCCAACGGT GAAAGTTACT 
GGACGTGAAA ACATTAATAA GTTTTTTTCT AGAAATGATG CCAAAGTTAG GAGCTTGAAA 
CTAAAATTAG ATACTATAGA TTTCCAGTAC ACTGGGCACT TGCACAAAAG CATTCTGATC 
ATGGCCACGG GTGAAATGTT TTGGACAGGA ACTCCGGTCT ATAAGTTTTG TCAAACATTT 
ATCCTGCTAC CATCCAGCAA TGGGTCTACG TTTGACATTA CCAATGATAT AATCAGATTT 
ATTTCCAATT CATTCAAGCC TTATGTCTTA ACAGATGCTT CTTTGTCACA ATCTAACGAG 
GAGAACTCTG TCAGTGCAGT TGAAGAAGAT AAAATAAGGC ATGAGTCCGG AGTTGAAAAA 
GAAAAAGAAA AAGAAAAATC ACCAGAAATA TCGAAACCAA AGGCCAAAAA GGAAACGGTC 
AAAGACACAA CTGCGCCAAC TGAATCGTCA ACTCAGGAAA AACCAATTGT CGATCATAGT 
CAACCTCGGG CTATACCCGT CACTAAAGAG TCCAAGATTC ACACCGAAAC GGTACCATCT 
AGTACTAAGG GCAACCACAA ACAAGATGAG GTCTCTACAG AAGAGTTAGG AAACGTTACT 
AAACTAAATG AAAAATCTCA CAAAGCCGAA AAGAAAGCTG CACCAATCAA GACAAAGGAA 
GGCTCTGTTG AGGCCATAAA TGCTGTTAAT AACTCTTCTT TGCCCAACGG TAAAGAAGTT 
AGTGATGAAA AACCGGTACC TGGGGGCGTA AAGGAAGCTG AAACTGAAAT TAAACCAATA 
GAGCCACAGG TTTCCGACGC CAAAGAATCC GGCAATAACG CTTCAACACC TTCATCTTCT 
CCAGAGCCCG TTGCGAATCC ACCAAAGATG ACATGGGCCT CAAAGTTGAT GAATGAAAAC 
TCCGACAGAA TTAGCAAAAA TAATACCACG GTTGAATACA TCAGACCTGA AACACTACCC 
AAGAAACCGA CTGAAAGAAA ATTTGAAATG GGGAATCGGA GGGACAACGC TAGTGCTAAC 
AGCAAAAATA AGAAAAAACC AGTATTCAGC ACCGTTAACA AGGACGGCTT TTATCCAATC 
TACATTAGAG GTACAAATGG CTTAAGAGAA GAAAAATTGA GAAGTGCCTT AGAAAAAGAA 
TTCGGTAAAG TTATGAGGAT AACAGCTGCA GACAATTTTG CCGTCGTCGA CTTTGAAACT 
CAAAAGAGCC AAATCGATGC TTTAGAGAAG AAGAAAAAGT CAATTGATGG TATTGAAGTA 
TGCTTAGAAA GAAAAACAGT AAAGAAGCCC ACGAGCAATA ATCCTCCTGG CATATTTACT 
AATGGAACAC GTTCTCATAG AAAGCAACCC CTAAAAAGAA AGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Nature387(6632 Suppl)93-8(1997 May)
Philippsen P,Kleine K,P?hlmann R,D?sterh?ft A,Hamberg K,Hegemann JH,Obermaier B,Urrestarazu LA,Aert R,Albermann K,Altmann R,Andr? B,Baladron V,Ballesta JP,B?cam AM,Beinhauer J,Boskovic J,Buitrago MJ,Bussereau F,Coster F,Crouzet M,D'Angelo M,Dal Pero F,De Antoni A,Del Rey F,Doignon F,Domdey H,Dubois E,Fiedler T,Fleig U,Floeth M,Fritz C,Gaillardin C,Garcia-Cantalejo JM,Glansdorff NN,Goffeau A,Gueldener U,Herbert C,Heumann K,Heuss-Neitzel D,Hilbert H,Hinni K,Iraqui Houssaini I,Jacquet M,Jimenez A,Jonniaux JL,Karpfinger L,Lanfranchi G,Lepingle A,Levesque H,Lyck R,Maftahi M,Mallet L,Maurer KC,Messenguy F,Mewes HW,M?sti D,Nasr F,Nicaud JM,Niedenthal RK,Pandolfo D,Pi?rard A,Piravandi E,Planta RJ,Pohl TM,Purnelle B,Rebischung C,Remacha M,Revuelta JL,Rinke M,Saiz JE,Sartorello F,Scherens B,Sen-Gupta M,Soler-Mira A,Urbanus JH,Valle G,Van Dyck L,Verhasselt P,Vierendeels F,Vissers S,Voet M,Volckaert G,Wach A,Wambutt R,Wedler H,Zollner A,Hani J

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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BRE5 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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