VPS27 cDNA ORF clone, Saccharomyces cerevisiae S288C

The following VPS27 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VPS27 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi04630 NM_001183183.3
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Saccharomyces cerevisiae S288C ESCRT-0 subunit protein VPS27 (VPS27), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSi04630
    Clone ID Related Accession (Same CDS sequence) NM_001183183.3
    Accession Version NM_001183183.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1869bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product ESCRT-0 subunit protein VPS27
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001146). On Jul 30, 2012 this sequence version replaced NM_001183183.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGTCCGTTA GCACGCCAAG TGAGTTAGAC GCATTAATAG AACAAGCCAC TAGTGAGAGC 
    ATTCCCAACG GTGACCTTGA TCTCCCCATA GCGTTGGAAA TTTCAGATGT GTTAAGATCT
    AGAAGGGTAA ATCCCAAAGA TTCTATGCGT TGTATAAAGA AAAGGATTCT CAATACGGCC
    GATAATCCAA ATACCCAATT GTCTTCCTGG AAGCTTACCA ATATATGTGT AAAAAATGGT
    GGAACGCCTT TTATTAAGGA AATTTGTTCC AGAGAGTTCA TGGATACCAT GGAACATGTC
    ATTTTAAGAG AAGATAGCAA CGAAGAACTT TCTGAGCTGG TAAAGACCAT ATTATATGAA
    CTATACGTAG CCTTCAAAAA TGATTCGCAA CTTAACTATG TGGCAAAAGT TTATGATAAA
    TTAATCTCTC GTGGCATTAA ATTCCCTGAA AAGCTAACAC TTTCCAATTC TCCGACTGCA
    ATGTTTGACT CGAAGACGCC CGCAGATTGG ATTGATTCTG ATGCTTGTAT GATTTGCTCC
    AAAAAGTTTT CTCTACTAAA TAGGAAACAT CACTGTCGTT CCTGTGGTGG AGTTTTCTGC
    CAAGAACACT CATCAAATAG TATACCCTTA CCTGATTTGG GTATATATGA GCCAGTCAGA
    GTATGTGATA GCTGCTTTGA AGATTACGAT TTGAAGAGAC ATGACGACAG TAAAAAATCT
    AAAAAACATC GCCATAAAAG AAAGAAGGAC AGGGACTACT CAACTCCTGA GGATGAAGAA
    GAGCTGATAA GGAAAGCAAT AGAACTCTCC TTGAAAGAAT CTAGAAATAG CGCTAGTAGC
    GAGCCTATTG TCCCCGTTGT AGAATCAAAG AATGAGGTGA AGAGACAAGA AATAGAAGAA
    GAAGAAGATC CTGATTTAAA AGCTGCTATT CAGGAAAGCT TGAGAGAAGC TGAAGAAGCG
    AAGCTACGCA GTGAGCGTCA AAAAGCCTCT AGACAGATGC AACCCCAACA GCCATCACCT
    CAACCTCAAC CTATCCATTC CGTTGATTTA TCTGATGAGG AGAAAGACAG CATATACATG
    TTTGCTTCAC TTGTCGAAAA AATGAAATCG AGGCCCTTGA ACGAAATTTT GGAAGACTCT
    AAATTACAAA ACTTGGCTCA AAGAGTATTT GCTTCCAAAG CAAGGTTGAA TTATGCTTTA
    AATGATAAAG CTCAGAAATA CAATACTTTG ATAGAAATGA ACGGCAAGAT TTCGGAAATT
    ATGAACATAT ACGATAGATT ATTGGAGCAA CAGCTACAAA GCATCAATTT GTCTCAGCAA
    TACACACTTC CACAAGTCCC TTCTGATCCG TACAATTACC TAACAGAAAA TGTGCAAAAT
    CCAGCAGAAA GCTATCAAAC ACCTCCCCTC CAGCAACTAT CATCTCATCA ATACAAACCA
    CAACAGGATG TCTCACGCCA ACAATCGGTA AAAGCAAATT CATCACCTAC CACTAATATT
    GATCACTTGA AAACCATCGA TGTGACACCA CACGCACAGC AAAAGCCTCA ATCGCACGTT
    GAACTTGCAC CTTCGGACCC ACCTTATCCA AAAGAAGAGG CCGAAGATGA AGGAACACAG
    GCAGTGCAAG ATGAGGAATC CTCCACACAA GAATCCCGCG AAAGGCCGTA TCCAGTAGAA
    ACAGAGAATG GTGAAACTTC AATAAATAAA CGGCCTCAAG GCATTACACG TTACGATTTT
    CCAACTGTTC CTGCACGCAA ATTCGTCCAA CCGGAGTCAA CTGTACCACT TCCTGCAAGT
    TCCTCTGAAA TTCCGATCAA AGAGGAAAGG CCGCCTAGTC CTCAAGAGGA ACTGCTAATA
    GAGCTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_014403.3
    CDS1..1869
    Translation

    Target ORF information:

    RefSeq Version NM_001183183.3
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C ESCRT-0 subunit protein VPS27 (VPS27), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001183183.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGTCCGTTA GCACGCCAAG TGAGTTAGAC GCATTAATAG AACAAGCCAC TAGTGAGAGC 
    ATTCCCAACG GTGACCTTGA TCTCCCCATA GCGTTGGAAA TTTCAGATGT GTTAAGATCT
    AGAAGGGTAA ATCCCAAAGA TTCTATGCGT TGTATAAAGA AAAGGATTCT CAATACGGCC
    GATAATCCAA ATACCCAATT GTCTTCCTGG AAGCTTACCA ATATATGTGT AAAAAATGGT
    GGAACGCCTT TTATTAAGGA AATTTGTTCC AGAGAGTTCA TGGATACCAT GGAACATGTC
    ATTTTAAGAG AAGATAGCAA CGAAGAACTT TCTGAGCTGG TAAAGACCAT ATTATATGAA
    CTATACGTAG CCTTCAAAAA TGATTCGCAA CTTAACTATG TGGCAAAAGT TTATGATAAA
    TTAATCTCTC GTGGCATTAA ATTCCCTGAA AAGCTAACAC TTTCCAATTC TCCGACTGCA
    ATGTTTGACT CGAAGACGCC CGCAGATTGG ATTGATTCTG ATGCTTGTAT GATTTGCTCC
    AAAAAGTTTT CTCTACTAAA TAGGAAACAT CACTGTCGTT CCTGTGGTGG AGTTTTCTGC
    CAAGAACACT CATCAAATAG TATACCCTTA CCTGATTTGG GTATATATGA GCCAGTCAGA
    GTATGTGATA GCTGCTTTGA AGATTACGAT TTGAAGAGAC ATGACGACAG TAAAAAATCT
    AAAAAACATC GCCATAAAAG AAAGAAGGAC AGGGACTACT CAACTCCTGA GGATGAAGAA
    GAGCTGATAA GGAAAGCAAT AGAACTCTCC TTGAAAGAAT CTAGAAATAG CGCTAGTAGC
    GAGCCTATTG TCCCCGTTGT AGAATCAAAG AATGAGGTGA AGAGACAAGA AATAGAAGAA
    GAAGAAGATC CTGATTTAAA AGCTGCTATT CAGGAAAGCT TGAGAGAAGC TGAAGAAGCG
    AAGCTACGCA GTGAGCGTCA AAAAGCCTCT AGACAGATGC AACCCCAACA GCCATCACCT
    CAACCTCAAC CTATCCATTC CGTTGATTTA TCTGATGAGG AGAAAGACAG CATATACATG
    TTTGCTTCAC TTGTCGAAAA AATGAAATCG AGGCCCTTGA ACGAAATTTT GGAAGACTCT
    AAATTACAAA ACTTGGCTCA AAGAGTATTT GCTTCCAAAG CAAGGTTGAA TTATGCTTTA
    AATGATAAAG CTCAGAAATA CAATACTTTG ATAGAAATGA ACGGCAAGAT TTCGGAAATT
    ATGAACATAT ACGATAGATT ATTGGAGCAA CAGCTACAAA GCATCAATTT GTCTCAGCAA
    TACACACTTC CACAAGTCCC TTCTGATCCG TACAATTACC TAACAGAAAA TGTGCAAAAT
    CCAGCAGAAA GCTATCAAAC ACCTCCCCTC CAGCAACTAT CATCTCATCA ATACAAACCA
    CAACAGGATG TCTCACGCCA ACAATCGGTA AAAGCAAATT CATCACCTAC CACTAATATT
    GATCACTTGA AAACCATCGA TGTGACACCA CACGCACAGC AAAAGCCTCA ATCGCACGTT
    GAACTTGCAC CTTCGGACCC ACCTTATCCA AAAGAAGAGG CCGAAGATGA AGGAACACAG
    GCAGTGCAAG ATGAGGAATC CTCCACACAA GAATCCCGCG AAAGGCCGTA TCCAGTAGAA
    ACAGAGAATG GTGAAACTTC AATAAATAAA CGGCCTCAAG GCATTACACG TTACGATTTT
    CCAACTGTTC CTGCACGCAA ATTCGTCCAA CCGGAGTCAA CTGTACCACT TCCTGCAAGT
    TCCTCTGAAA TTCCGATCAA AGAGGAAAGG CCGCCTAGTC CTCAAGAGGA ACTGCTAATA
    GAGCTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
    Nature387(6632 Suppl)93-8(1997 May)
    Philippsen P,Kleine K,P?hlmann R,D?sterh?ft A,Hamberg K,Hegemann JH,Obermaier B,Urrestarazu LA,Aert R,Albermann K,Altmann R,Andr? B,Baladron V,Ballesta JP,B?cam AM,Beinhauer J,Boskovic J,Buitrago MJ,Bussereau F,Coster F,Crouzet M,D'Angelo M,Dal Pero F,De Antoni A,Del Rey F,Doignon F,Domdey H,Dubois E,Fiedler T,Fleig U,Floeth M,Fritz C,Gaillardin C,Garcia-Cantalejo JM,Glansdorff NN,Goffeau A,Gueldener U,Herbert C,Heumann K,Heuss-Neitzel D,Hilbert H,Hinni K,Iraqui Houssaini I,Jacquet M,Jimenez A,Jonniaux JL,Karpfinger L,Lanfranchi G,Lepingle A,Levesque H,Lyck R,Maftahi M,Mallet L,Maurer KC,Messenguy F,Mewes HW,M?sti D,Nasr F,Nicaud JM,Niedenthal RK,Pandolfo D,Pi?rard A,Piravandi E,Planta RJ,Pohl TM,Purnelle B,Rebischung C,Remacha M,Revuelta JL,Rinke M,Saiz JE,Sartorello F,Scherens B,Sen-Gupta M,Soler-Mira A,Urbanus JH,Valle G,Van Dyck L,Verhasselt P,Vierendeels F,Vissers S,Voet M,Volckaert G,Wach A,Wambutt R,Wedler H,Zollner A,Hani J


    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG