ASI3 cDNA ORF clone, Saccharomyces cerevisiae S288C

The following ASI3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ASI3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi04618 NM_001182847.2
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Saccharomyces cerevisiae S288C putative ubiquitin-protein ligase ASI3 (ASI3), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSi04618
    Clone ID Related Accession (Same CDS sequence) NM_001182847.2
    Accession Version NM_001182847.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2031bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product putative ubiquitin-protein ligase ASI3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001146). On Apr 26, 2011 this sequence version replaced NM_001182847.1. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGTCTACAA ATATATTGCA ACATGTTAAG CAATTGCTCC ATAACAGAGA TGTATTTAGC 
    TTTTTTCACA ACAAGACTGG AAATCTTAAT TACCTAGATA ACACAACTCA GAAACCGGAA
    GTTTTTGTAA GTCCAAATTC TACAATAGTT TCAGCCCCAA CTTTGGACTC TTTTCAAGCA
    TTGATGGAGA AAGGTAACTT CACCACTTTG CAACTAGCTA AGGTAGGAAT TAGAATGTTT
    TTCTCCTATT CGGTAAGTAA GTATGCTGTT CTATGTTTTA GTACCGCCAT TATTTTGAAT
    AGGTTGACTG TAATGTCTTC ATTGAGATCA AATTCTACAA ACATTCGTTT ACCTCTTTGG
    TCAAAAACTT TGCTCCATTT AGTAGCCACT CTTTCACTGG TTAAAGCACT TTTACAAATT
    CTGAGTCAAT TTGGACTCAT GCATGAACTG CACGTGAGTG ATACAGATTT TTATGCTTTA
    AGTGTCTATT TATTTGTAGC TCTGTCTGAC TGCATTGAAA TTTTTATTTC TTCAACGACG
    AACGTTCCTT CATTAATATG CTCTGATTTT TCCATATGGG GTTTATCATT GAATCTTTAT
    ATCATATCCA AAATGCCCGC GGGGCAGCAG CATATTGGCG ATAATGTTGA ACTACTAGGA
    GCGGTTTTTC ACAGATTAGT AATTCATTTG GTAGAATTGT TTCATATAAG AGCCTATAGA
    TTATGTGGAG AAGTTATATT AAACGCGGGT TTTTTTACCG CATTCGTCAC TAGGACCTAT
    TTGAACGGAT TAGATTTTAT TAACATCTGT CTCATTCACA ATTATTTCCC TGGCTTTTTC
    TATATTTCCA CCATATTACT GGCGTCTATA GGCATTTTCC TGAAGGCTTT GTTCACCAGT
    AATCCGTTCC GATCATTATA TTCAAGATAT AAAAACTTGG AAAAATGGTG GAGATCAAAT
    AATTACAATG GAGAAGAGGA GTTTAACGAA ATAGCTCTTT CATTGTGTCT ACTTTTAACG
    TCGAATGACT ATAAAATATT TAAGAAATCA GACAACGTAA AATCCGTTGA TGAAGTCGCT
    GCTTTCAGTA ATAGTTATGT GGTTAGTGGA CATTTAAACC AACTCCAGTC GACACCAGAG
    GATCTGTTAT CTCGTAAGGA AATGACTACC GATTCCCAGT TACCAGGTTT TGCGAGGACA
    TATTTAGGCC TTTTTGAATT AGTAAGAACC ATTATTTTGA CGTATTCTAG ACTCCTAAAA
    AATCTTCTGT GGAGTAAAAA TTTTGAGAGC AGTATAGATA AGAAACCGAG AGTGGGAAAG
    CGTAAAAAGA GGGATTTAAA CAAATATGTT ACAGAAAAAA ACTATAAAAA GTTTTTATAT
    AAACCGGACG TCAAAGAGCT TAATATTGAG AGTGATCTCA GAAGCCTGGA GTTATTGCTT
    CCTGAAGACG ATTCATCAAA AGACTACTTT CCCCCCAGAA AAATCGATGA AAGTGTTTCA
    GATGAAGAGT TTGATAGTGA TATGGAAAGT CAGTTGATTA TTGATGAAGA AAAAGAATTA
    ACACATTTAT CAAGTAATGC TGTCGATTCT GATGATTTAG AGGAAATAGC CTGGAACATC
    TCCATGTGGT CTATTTTAAA TTATGAAATG GATGTCCATA ATAAGGTTAA TGGACCGTTG
    ACTAGATCAC AATATGGTAA AAGAAACCCA CAAGGTGTAC TAGTCGATGT TGTTATTGAA
    CGCCTTCTGC ATCATACAAA CTCCAGGTAT ATGTACAAAC GTTTAAATAT GAAGGACGAT
    GACAAATTAG AGTTCAAGTT TGATTTTGCC TTTGATAGTT GCGACGAAGT GGAGGAAATG
    GATCTCTCCT GCCTAATCTG CAAAGTTAAT AAAAGAAATA TTGTAACTTG GCCCTGTAGA
    TGCCTTGCAT TATGCGATGA TTGTAGGATA TCTCTGGGTT ATAAAGGATT TGCTACATGC
    GTCAGCTGTG ATAGTGAGGT GAAGGGTTAC AGTAAGTTGA ATATAGTCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_014390.2
    CDS1..2031
    Translation

    Target ORF information:

    RefSeq Version NM_001182847.2
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C putative ubiquitin-protein ligase ASI3 (ASI3), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001182847.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGTCTACAA ATATATTGCA ACATGTTAAG CAATTGCTCC ATAACAGAGA TGTATTTAGC 
    TTTTTTCACA ACAAGACTGG AAATCTTAAT TACCTAGATA ACACAACTCA GAAACCGGAA
    GTTTTTGTAA GTCCAAATTC TACAATAGTT TCAGCCCCAA CTTTGGACTC TTTTCAAGCA
    TTGATGGAGA AAGGTAACTT CACCACTTTG CAACTAGCTA AGGTAGGAAT TAGAATGTTT
    TTCTCCTATT CGGTAAGTAA GTATGCTGTT CTATGTTTTA GTACCGCCAT TATTTTGAAT
    AGGTTGACTG TAATGTCTTC ATTGAGATCA AATTCTACAA ACATTCGTTT ACCTCTTTGG
    TCAAAAACTT TGCTCCATTT AGTAGCCACT CTTTCACTGG TTAAAGCACT TTTACAAATT
    CTGAGTCAAT TTGGACTCAT GCATGAACTG CACGTGAGTG ATACAGATTT TTATGCTTTA
    AGTGTCTATT TATTTGTAGC TCTGTCTGAC TGCATTGAAA TTTTTATTTC TTCAACGACG
    AACGTTCCTT CATTAATATG CTCTGATTTT TCCATATGGG GTTTATCATT GAATCTTTAT
    ATCATATCCA AAATGCCCGC GGGGCAGCAG CATATTGGCG ATAATGTTGA ACTACTAGGA
    GCGGTTTTTC ACAGATTAGT AATTCATTTG GTAGAATTGT TTCATATAAG AGCCTATAGA
    TTATGTGGAG AAGTTATATT AAACGCGGGT TTTTTTACCG CATTCGTCAC TAGGACCTAT
    TTGAACGGAT TAGATTTTAT TAACATCTGT CTCATTCACA ATTATTTCCC TGGCTTTTTC
    TATATTTCCA CCATATTACT GGCGTCTATA GGCATTTTCC TGAAGGCTTT GTTCACCAGT
    AATCCGTTCC GATCATTATA TTCAAGATAT AAAAACTTGG AAAAATGGTG GAGATCAAAT
    AATTACAATG GAGAAGAGGA GTTTAACGAA ATAGCTCTTT CATTGTGTCT ACTTTTAACG
    TCGAATGACT ATAAAATATT TAAGAAATCA GACAACGTAA AATCCGTTGA TGAAGTCGCT
    GCTTTCAGTA ATAGTTATGT GGTTAGTGGA CATTTAAACC AACTCCAGTC GACACCAGAG
    GATCTGTTAT CTCGTAAGGA AATGACTACC GATTCCCAGT TACCAGGTTT TGCGAGGACA
    TATTTAGGCC TTTTTGAATT AGTAAGAACC ATTATTTTGA CGTATTCTAG ACTCCTAAAA
    AATCTTCTGT GGAGTAAAAA TTTTGAGAGC AGTATAGATA AGAAACCGAG AGTGGGAAAG
    CGTAAAAAGA GGGATTTAAA CAAATATGTT ACAGAAAAAA ACTATAAAAA GTTTTTATAT
    AAACCGGACG TCAAAGAGCT TAATATTGAG AGTGATCTCA GAAGCCTGGA GTTATTGCTT
    CCTGAAGACG ATTCATCAAA AGACTACTTT CCCCCCAGAA AAATCGATGA AAGTGTTTCA
    GATGAAGAGT TTGATAGTGA TATGGAAAGT CAGTTGATTA TTGATGAAGA AAAAGAATTA
    ACACATTTAT CAAGTAATGC TGTCGATTCT GATGATTTAG AGGAAATAGC CTGGAACATC
    TCCATGTGGT CTATTTTAAA TTATGAAATG GATGTCCATA ATAAGGTTAA TGGACCGTTG
    ACTAGATCAC AATATGGTAA AAGAAACCCA CAAGGTGTAC TAGTCGATGT TGTTATTGAA
    CGCCTTCTGC ATCATACAAA CTCCAGGTAT ATGTACAAAC GTTTAAATAT GAAGGACGAT
    GACAAATTAG AGTTCAAGTT TGATTTTGCC TTTGATAGTT GCGACGAAGT GGAGGAAATG
    GATCTCTCCT GCCTAATCTG CAAAGTTAAT AAAAGAAATA TTGTAACTTG GCCCTGTAGA
    TGCCTTGCAT TATGCGATGA TTGTAGGATA TCTCTGGGTT ATAAAGGATT TGCTACATGC
    GTCAGCTGTG ATAGTGAGGT GAAGGGTTAC AGTAAGTTGA ATATAGTCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
    Nature387(6632 Suppl)93-8(1997 May)
    Philippsen P,Kleine K,P?hlmann R,D?sterh?ft A,Hamberg K,Hegemann JH,Obermaier B,Urrestarazu LA,Aert R,Albermann K,Altmann R,Andr? B,Baladron V,Ballesta JP,B?cam AM,Beinhauer J,Boskovic J,Buitrago MJ,Bussereau F,Coster F,Crouzet M,D'Angelo M,Dal Pero F,De Antoni A,Del Rey F,Doignon F,Domdey H,Dubois E,Fiedler T,Fleig U,Floeth M,Fritz C,Gaillardin C,Garcia-Cantalejo JM,Glansdorff NN,Goffeau A,Gueldener U,Herbert C,Heumann K,Heuss-Neitzel D,Hilbert H,Hinni K,Iraqui Houssaini I,Jacquet M,Jimenez A,Jonniaux JL,Karpfinger L,Lanfranchi G,Lepingle A,Levesque H,Lyck R,Maftahi M,Mallet L,Maurer KC,Messenguy F,Mewes HW,M?sti D,Nasr F,Nicaud JM,Niedenthal RK,Pandolfo D,Pi?rard A,Piravandi E,Planta RJ,Pohl TM,Purnelle B,Rebischung C,Remacha M,Revuelta JL,Rinke M,Saiz JE,Sartorello F,Scherens B,Sen-Gupta M,Soler-Mira A,Urbanus JH,Valle G,Van Dyck L,Verhasselt P,Vierendeels F,Vissers S,Voet M,Volckaert G,Wach A,Wambutt R,Wedler H,Zollner A,Hani J


    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG