FKH2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	FKH2
Entrez Gene ID	855656
Full Name	forkhead family transcription factor FKH2
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182906.3	NP_014331.3	forkhead family transcription factor FKH2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Philippsen P, Kleine K, P?hlmann R, D?sterh?ft A, Hamberg K, Hegemann JH, Obermaier B, Urrestarazu LA, Aert R, Albermann K, Altmann R, Andr? B, Baladron V, Ballesta JP, B?cam AM, Beinhauer J, Boskovic J, Buitrago MJ, Bussereau F, Coster F, Crouzet M, D'Angelo M, Dal Pero F, De Antoni A, Del Rey F, Doignon F, Domdey H, Dubois E, Fiedler T, Fleig U, Floeth M, Fritz C, Gaillardin C, Garcia-Cantalejo JM, Glansdorff NN, Goffeau A, Gueldener U, Herbert C, Heumann K, Heuss-Neitzel D, Hilbert H, Hinni K, Iraqui Houssaini I, Jacquet M, Jimenez A, Jonniaux JL, Karpfinger L, Lanfranchi G, Lepingle A, Levesque H, Lyck R, Maftahi M, Mallet L, Maurer KC, Messenguy F, Mewes HW, M?sti D, Nasr F, Nicaud JM, Niedenthal RK, Pandolfo D, Pi?rard A, Piravandi E, Planta RJ, Pohl TM, Purnelle B, Rebischung C, Remacha M, Revuelta JL, Rinke M, Saiz JE, Sartorello F, Scherens B, Sen-Gupta M, Soler-Mira A, Urbanus JH, Valle G, Van Dyck L, Verhasselt P, Vierendeels F, Vissers S, Voet M, Volckaert G, Wach A, Wambutt R, Wedler H, Zollner A, Hani J
Nature387(6632 Suppl)93-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

It was found that ABF1, FKH2, and REB1 overexpression decreased the growth of strains in a prion-dependent manner and, thus, might influence [PSI;(+)] prion toxicity.
Title: [Identification of new genes that affect [PSI^(+)] prion toxicity in Saccharomyces cerevisiae yeast].

This study establishes the FKH1 and FKH2 role as evolutionarily conserved regulators of lifespan in yeast and identifies the APC as a novel component of this mechanism
Title: The yeast forkhead transcription factors fkh1 and fkh2 regulate lifespan and stress response together with the anaphase-promoting complex.

Study shows that Fkh1 and Fkh2 regulate the initiation timing of most of the earliest origins in the yeast genome through a novel mechanism involving origin clustering in G1 phase.
Title: Forkhead transcription factors establish origin timing and long-range clustering in S. cerevisiae.

The Mcm1-Fkh2 complex, first shown to transactivate genes within the CLB2 cluster that drive G(2)/M progression, also associated directly at the PHO5 promoter in a cell cycle-dependent manner.
Title: Coupling phosphate homeostasis to cell cycle-specific transcription: mitotic activation of Saccharomyces cerevisiae PHO5 by Mcm1 and Forkhead proteins.

Chromatin-mediated repression by Isw1 and Isw2 may serve to limit activation of CLB2 expression by the Clb2/Cdk1 kinase during G(2)/M and to fully repress expression during G(1).
Title: The Isw2 chromatin-remodeling ATPase cooperates with the Fkh2 transcription factor to repress transcription of the B-type cyclin gene CLB2.

The following FKH2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FKH2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04563	NM_001182906.3 Latest version!	Saccharomyces cerevisiae S288C forkhead family transcription factor FKH2 (FKH2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FKH2 ( NM_001182906.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_014331.3 Saccharomyces cerevisiae S288C forkhead family transcription factor FKH2 (FKH2), partial mRNA.

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CloneID	OSi04563
Clone ID Related Accession (Same CDS sequence)	NM_001182906.3
Accession Version	NM_001182906.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	forkhead family transcription factor FKH2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001146). On Jul 30, 2012 this sequence version replaced NM_001182906.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCCAGCA GCAATTTTAA CGAGATGAAC GAACTAAATA TGACTCAAAC AAATTATGGC 
AGCACAAAAT ATACCGCTCA GCATCATCAA GGTGTAATAA ATGCCATCAT CTCTTCACTG 
ACAGCCCCAG ATCAGCCAAC TACCGTATCA TTACAATATT CCAACGACAA GAACATGGCG 
ACGGAAATAC AAGCTTATGC TAAACTCTCC GGACCAAACT GGACCTATTA TGTGAAGGAT 
TTGGAAGTTT CTATTGGTAG AAATACTGAT CCCTTAAACA GTGCACTACA GGAAAATTCA 
GATGGTGTAA AAAACTCATA TAGAGTGAAT ATTGATCTCG GACCTGCCAA AGTCGTTTCT 
AGAAAGCACG CCATAATAAA ATACAACATG AATATCGGCG GTTGGGAATT GCACATATTG 
GGCCGTAACG GCGCTAAAGT AAACTTTCAA AGGACTCATA ATGGACCCAA CAATCCTCCC 
ATTCGATTAT CTTCCGGAAC CCTGCTAGAC ATAGGAGGAA CACAAATGAT GTTTATCCTG 
CCCGACAGTG ACCCGGTTGT TGCTCCTATT TGTATAGAAC ACCTGATGCC TAATTTAATA 
AACATGTTCG GATTGGAAGG CAACAACAAC CCCCTGCTAA GAGACATTAT AAAACAGTCT 
AATTATGCTA AGCAAAGGCA ATTAACCTCT AATCAACAAA TCAAAGGGTT TAAGCTTTAT 
GGAAGTGGTG GCAATGCTCC TTTTGGTAGC GGAGCGAACT TGGGCCCTAG CGAACAAGGC 
ATCTTCAACA ACAATAATAA TAGTAAGAAC AAGAATGGCT ACTTTACGTC CATAAACCCA 
AACTATACAG CATCTACAAC AACAAGCAAT ACCATCAATC CACAAGCGGC TTCTCCACAG 
GGTCCTCCAA ATACCATCAT CGCTGCAAAT TTTGTAGATT CATACAAGTC ATCAAATGCC 
TATCCTCAAG CTTTGGATTT TACTTCGGAC CTATCCCATG ACGAAAACAG GAACGTTAAA 
CCACCTCATT CATACGCAAC TATGATAACA CAGGCCATAC TGTCGTCGCC GGAGGGAGTT 
ATTTCACTAG CCGATATCTA CAAGTATATT TCTTCCAATT ATGCATACTA CAGGTTTGCT 
AAATCCGGTT GGCAAAACTC CATTAGGCAT AATTTATCAT TAAATAAGGC GTTTGAAAAG 
GTTCCGAGAA GACCCAATGA GCCGGGCAAA GGTATGAAAT GGAGAATTAG CGAATCTTAT 
CAGCAAGAAT TTCTAAACAA GTGGAATACC GGAAAAGTGG GGAAAATCAG GCGGGGATCC 
TCAGTAGCAA GGCAACTACA GTTACATATG GCAAAATTCA ACAGTTTACC CATGGAAATG 
GACTATAGGT TATCGCTTAA CATGGCTCAA CCCCCTAAAC GACAACTACA ATCTCACAAT 
GTGCTAGAAC CATCCAACAA TAACATCATA GAAGGGTTCG TACAACATGT CCCATCCAAA 
GGTAACCTTC CTGCATCGCA ACAATCACAG CCCCCTGTGT CACATCAAAA TCAGTCACAG 
CAGCCGCCAC CACAGGAACA ACGTCAAGAG ATTCAATTTA CCTTCGCGGA TACCCAAAAT 
CGAAATATAG CGTTAGCAAG ACCTATCAAG ACGCCACAAT TGCAAGCTCC CAATTCAAAT 
GCTAACTTAA ATCAAAACAA TATGAAAGAA TACAAAGAGT CACTTCATCC GCCTGCAATA 
TCAATATCAC AAATGAATAG GCAATCTCCA AACAACGCTC TAGTCTCGTT CACTAATGCA 
TGTGCTAATT CCAAAATAAT AAACAACATC AGCGATAGCG CCGACAAATC GACAAACAAT 
AACGGCGGTA CCAAAATGAA TCTACCCGCC ATATCTACTT CTTCACTTGA TGAAAATGGT 
AATTTGGAGC CGACAACTAC CACGTCTTCC GGTAATTCTA ACTCAGTTCC GCAAACTGGA 
ACAACAACTT CCTCATTGGC AGCGAACAGT CTACGCCTTT CTCAACCATA TGACACGCTA 
TTACGATCTC CTACTAAGGC ATTTCATATT ACTGCGATGG AAGCATATAC ACCAGAAAGA 
GGAAGCGCTA ATAGGGCTAG GTCACCTCTT CATAGTAATA GCAACAATAC TAATAATAAC 
GGCGCAAATA ATTCTAATTT ACAGACTAGC GGAATGGAAA ACAAACAAAC AGGTCTAGTA 
TTGGATTCTA ATGTCTTGAA AAGCATGGAA TCAAACAATG ATAATAGACG GCTGACACCA 
TCAACAAGTA AATCGCAGAA TGTCAAAAGT TCTCCAGGTG TATGGAATTT ATTGCAGTTT 
AGCTCAACAA ACAACACTCC TGCCGCAGAT AGCGGAGGCA ACAAGAGAGG ATTTTCCATA 
AATCCGGACA TTAAAGCCAA AGAAAACGAG AATGCTACTT CAGAAAAAGA TTCGGACTCT 
AACTCAAATG ATCTGGAAAC TAAAGATATT AATTCTTCAC CTTTGAAAAA CCAAGGTGGT 
AGCACCGCCA ATGCCAAGGA ACTAATACTA GATACGGATG GTGCAAAGAT CAGTATTATC 
AACAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_014331.3
CDS	1..2589
Translation	MSSSNFNEMNELNMTQTNYGSTKYTAQHHQGVINAIISSLTAPDQPTTVSLQYSNDKNMATEIQAYAKLSGPNWTYYVKDLEVSIGRNTDPLNSALQENSDGVKNSYRVNIDLGPAKVVSRKHAIIKYNMNIGGWELHILGRNGAKVNFQRTHNGPNNPPIRLSSGTLLDIGGTQMMFILPDSDPVVAPICIEHLMPNLINMFGLEGNNNPLLRDIIKQSNYAKQRQLTSNQQIKGFKLYGSGGNAPFGSGANLGPSEQGIFNNNNNSKNKNGYFTSINPNYTASTTTSNTINPQAASPQGPPNTIIAANFVDSYKSSNAYPQALDFTSDLSHDENRNVKPPHSYATMITQAILSSPEGVISLADIYKYISSNYAYYRFAKSGWQNSIRHNLSLNKAFEKVPRRPNEPGKGMKWRISESYQQEFLNKWNTGKVGKIRRGSSVARQLQLHMAKFNSLPMEMDYRLSLNMAQPPKRQLQSHNVLEPSNNNIIEGFVQHVPSKGNLPASQQSQPPVSHQNQSQQPPPQEQRQEIQFTFADTQNRNIALARPIKTPQLQAPNSNANLNQNNMKEYKESLHPPAISISQMNRQSPNNALVSFTNACANSKIINNISDSADKSTNNNGGTKMNLPAISTSSLDENGNLEPTTTTSSGNSNSVPQTGTTTSSLAANSLRLSQPYDTLLRSPTKAFHITAMEAYTPERGSANRARSPLHSNSNNTNNNGANNSNLQTSGMENKQTGLVLDSNVLKSMESNNDNRRLTPSTSKSQNVKSSPGVWNLLQFSSTNNTPAADSGGNKRGFSINPDIKAKENENATSEKDSDSNSNDLETKDINSSPLKNQGGSTANAKELILDTDGAKISIINN

Target ORF information:

RefSeq Version	NM_001182906.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C forkhead family transcription factor FKH2 (FKH2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182906.3

ORF Insert Sequence:

ATGTCCAGCA GCAATTTTAA CGAGATGAAC GAACTAAATA TGACTCAAAC AAATTATGGC 
AGCACAAAAT ATACCGCTCA GCATCATCAA GGTGTAATAA ATGCCATCAT CTCTTCACTG 
ACAGCCCCAG ATCAGCCAAC TACCGTATCA TTACAATATT CCAACGACAA GAACATGGCG 
ACGGAAATAC AAGCTTATGC TAAACTCTCC GGACCAAACT GGACCTATTA TGTGAAGGAT 
TTGGAAGTTT CTATTGGTAG AAATACTGAT CCCTTAAACA GTGCACTACA GGAAAATTCA 
GATGGTGTAA AAAACTCATA TAGAGTGAAT ATTGATCTCG GACCTGCCAA AGTCGTTTCT 
AGAAAGCACG CCATAATAAA ATACAACATG AATATCGGCG GTTGGGAATT GCACATATTG 
GGCCGTAACG GCGCTAAAGT AAACTTTCAA AGGACTCATA ATGGACCCAA CAATCCTCCC 
ATTCGATTAT CTTCCGGAAC CCTGCTAGAC ATAGGAGGAA CACAAATGAT GTTTATCCTG 
CCCGACAGTG ACCCGGTTGT TGCTCCTATT TGTATAGAAC ACCTGATGCC TAATTTAATA 
AACATGTTCG GATTGGAAGG CAACAACAAC CCCCTGCTAA GAGACATTAT AAAACAGTCT 
AATTATGCTA AGCAAAGGCA ATTAACCTCT AATCAACAAA TCAAAGGGTT TAAGCTTTAT 
GGAAGTGGTG GCAATGCTCC TTTTGGTAGC GGAGCGAACT TGGGCCCTAG CGAACAAGGC 
ATCTTCAACA ACAATAATAA TAGTAAGAAC AAGAATGGCT ACTTTACGTC CATAAACCCA 
AACTATACAG CATCTACAAC AACAAGCAAT ACCATCAATC CACAAGCGGC TTCTCCACAG 
GGTCCTCCAA ATACCATCAT CGCTGCAAAT TTTGTAGATT CATACAAGTC ATCAAATGCC 
TATCCTCAAG CTTTGGATTT TACTTCGGAC CTATCCCATG ACGAAAACAG GAACGTTAAA 
CCACCTCATT CATACGCAAC TATGATAACA CAGGCCATAC TGTCGTCGCC GGAGGGAGTT 
ATTTCACTAG CCGATATCTA CAAGTATATT TCTTCCAATT ATGCATACTA CAGGTTTGCT 
AAATCCGGTT GGCAAAACTC CATTAGGCAT AATTTATCAT TAAATAAGGC GTTTGAAAAG 
GTTCCGAGAA GACCCAATGA GCCGGGCAAA GGTATGAAAT GGAGAATTAG CGAATCTTAT 
CAGCAAGAAT TTCTAAACAA GTGGAATACC GGAAAAGTGG GGAAAATCAG GCGGGGATCC 
TCAGTAGCAA GGCAACTACA GTTACATATG GCAAAATTCA ACAGTTTACC CATGGAAATG 
GACTATAGGT TATCGCTTAA CATGGCTCAA CCCCCTAAAC GACAACTACA ATCTCACAAT 
GTGCTAGAAC CATCCAACAA TAACATCATA GAAGGGTTCG TACAACATGT CCCATCCAAA 
GGTAACCTTC CTGCATCGCA ACAATCACAG CCCCCTGTGT CACATCAAAA TCAGTCACAG 
CAGCCGCCAC CACAGGAACA ACGTCAAGAG ATTCAATTTA CCTTCGCGGA TACCCAAAAT 
CGAAATATAG CGTTAGCAAG ACCTATCAAG ACGCCACAAT TGCAAGCTCC CAATTCAAAT 
GCTAACTTAA ATCAAAACAA TATGAAAGAA TACAAAGAGT CACTTCATCC GCCTGCAATA 
TCAATATCAC AAATGAATAG GCAATCTCCA AACAACGCTC TAGTCTCGTT CACTAATGCA 
TGTGCTAATT CCAAAATAAT AAACAACATC AGCGATAGCG CCGACAAATC GACAAACAAT 
AACGGCGGTA CCAAAATGAA TCTACCCGCC ATATCTACTT CTTCACTTGA TGAAAATGGT 
AATTTGGAGC CGACAACTAC CACGTCTTCC GGTAATTCTA ACTCAGTTCC GCAAACTGGA 
ACAACAACTT CCTCATTGGC AGCGAACAGT CTACGCCTTT CTCAACCATA TGACACGCTA 
TTACGATCTC CTACTAAGGC ATTTCATATT ACTGCGATGG AAGCATATAC ACCAGAAAGA 
GGAAGCGCTA ATAGGGCTAG GTCACCTCTT CATAGTAATA GCAACAATAC TAATAATAAC 
GGCGCAAATA ATTCTAATTT ACAGACTAGC GGAATGGAAA ACAAACAAAC AGGTCTAGTA 
TTGGATTCTA ATGTCTTGAA AAGCATGGAA TCAAACAATG ATAATAGACG GCTGACACCA 
TCAACAAGTA AATCGCAGAA TGTCAAAAGT TCTCCAGGTG TATGGAATTT ATTGCAGTTT 
AGCTCAACAA ACAACACTCC TGCCGCAGAT AGCGGAGGCA ACAAGAGAGG ATTTTCCATA 
AATCCGGACA TTAAAGCCAA AGAAAACGAG AATGCTACTT CAGAAAAAGA TTCGGACTCT 
AACTCAAATG ATCTGGAAAC TAAAGATATT AATTCTTCAC CTTTGAAAAA CCAAGGTGGT 
AGCACCGCCA ATGCCAAGGA ACTAATACTA GATACGGATG GTGCAAAGAT CAGTATTATC 
AACAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Nature387(6632 Suppl)93-8(1997 May)
Philippsen P,Kleine K,P?hlmann R,D?sterh?ft A,Hamberg K,Hegemann JH,Obermaier B,Urrestarazu LA,Aert R,Albermann K,Altmann R,Andr? B,Baladron V,Ballesta JP,B?cam AM,Beinhauer J,Boskovic J,Buitrago MJ,Bussereau F,Coster F,Crouzet M,D'Angelo M,Dal Pero F,De Antoni A,Del Rey F,Doignon F,Domdey H,Dubois E,Fiedler T,Fleig U,Floeth M,Fritz C,Gaillardin C,Garcia-Cantalejo JM,Glansdorff NN,Goffeau A,Gueldener U,Herbert C,Heumann K,Heuss-Neitzel D,Hilbert H,Hinni K,Iraqui Houssaini I,Jacquet M,Jimenez A,Jonniaux JL,Karpfinger L,Lanfranchi G,Lepingle A,Levesque H,Lyck R,Maftahi M,Mallet L,Maurer KC,Messenguy F,Mewes HW,M?sti D,Nasr F,Nicaud JM,Niedenthal RK,Pandolfo D,Pi?rard A,Piravandi E,Planta RJ,Pohl TM,Purnelle B,Rebischung C,Remacha M,Revuelta JL,Rinke M,Saiz JE,Sartorello F,Scherens B,Sen-Gupta M,Soler-Mira A,Urbanus JH,Valle G,Van Dyck L,Verhasselt P,Vierendeels F,Vissers S,Voet M,Volckaert G,Wach A,Wambutt R,Wedler H,Zollner A,Hani J

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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FKH2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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