EAF7 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	EAF7
Entrez Gene ID	855586
Full Name	Eaf7p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182974.1	NP_014263.1	Eaf7p

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Philippsen P, Kleine K, P?hlmann R, D?sterh?ft A, Hamberg K, Hegemann JH, Obermaier B, Urrestarazu LA, Aert R, Albermann K, Altmann R, Andr? B, Baladron V, Ballesta JP, B?cam AM, Beinhauer J, Boskovic J, Buitrago MJ, Bussereau F, Coster F, Crouzet M, D'Angelo M, Dal Pero F, De Antoni A, Del Rey F, Doignon F, Domdey H, Dubois E, Fiedler T, Fleig U, Floeth M, Fritz C, Gaillardin C, Garcia-Cantalejo JM, Glansdorff NN, Goffeau A, Gueldener U, Herbert C, Heumann K, Heuss-Neitzel D, Hilbert H, Hinni K, Iraqui Houssaini I, Jacquet M, Jimenez A, Jonniaux JL, Karpfinger L, Lanfranchi G, Lepingle A, Levesque H, Lyck R, Maftahi M, Mallet L, Maurer KC, Messenguy F, Mewes HW, M?sti D, Nasr F, Nicaud JM, Niedenthal RK, Pandolfo D, Pi?rard A, Piravandi E, Planta RJ, Pohl TM, Purnelle B, Rebischung C, Remacha M, Revuelta JL, Rinke M, Saiz JE, Sartorello F, Scherens B, Sen-Gupta M, Soler-Mira A, Urbanus JH, Valle G, Van Dyck L, Verhasselt P, Vierendeels F, Vissers S, Voet M, Volckaert G, Wach A, Wambutt R, Wedler H, Zollner A, Hani J
Nature387(6632 Suppl)93-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In vitro binding assays showed that Eaf3/5/7 specifically stimulates NuA4 binding to di- and trimethylated histone H3 Lys-36 and that this binding is important for NuA4 occupancy in transcribed ORFs.
Title: The Eaf3/5/7 Subcomplex Stimulates NuA4 Interaction with Methylated Histone H3 Lys-36 and RNA Polymerase II.

Eaf5 and Eaf7 form a subcomplex. Eaf7 interacts with NuA4 through Eaf5.
Title: Functional dissection of the NuA4 histone acetyltransferase reveals its role as a genetic hub and that Eaf1 is essential for complex integrity.

The following EAF7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EAF7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04500	NM_001182974.1 Latest version!	Saccharomyces cerevisiae S288C Eaf7p (EAF7), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EAF7 ( NM_001182974.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_014263.1 Saccharomyces cerevisiae S288C Eaf7p (EAF7), partial mRNA.

Price & Availability↑

CloneID	OSi04500
Clone ID Related Accession (Same CDS sequence)	NM_001182974.1
Accession Version	NM_001182974.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Eaf7p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001146). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGTAGTAC ATTGGACAAT TGTGGATGAA ATACGACTTC TTAGATGGGC ATCTGAATTC 
AAACCGGCCG GAATTCATAA GCATTTCCAC ATGTTTTGCA TAGTGGAAAG AATGAATTCG 
CCAGATAAGT ATCCAGTGAC GCTATTACAG AAAGAAACCA TGAAGTTGGG GAAGGTATTT 
ACTGCAAAAG ACATATGGGA TAAGTTGAGC CAATCTTACA ATTTAGAGAA AATTGATGAA 
ATGGAGAATA CATATTCCTT GGAAGCCACT ACAGAGAGTT CACGGAATGG CAATGGAAAT 
GGTGACGATG CAGAAATTCA TGAAGAAACT TTACTTGAAT TGAATAATCG AATAAGAGTA 
CGCAAGCAAG ATTTTACATT ACCTTGGGAG GAATACGGTG AGTTGATCTT AGAAAATGCA 
AGAAAAAGTC CAAACTCTAA CGAAGAGTAT CCTCGAGTTG AAGATATGAA TGAGAAAGAT 
AGTACAATTC CAAAGGAAAG TCCTTCTACA GACCTAAAGA ACGACAACAA TAAACAGGAA 
AAAAATGCGA CAATTAAAGT GAAAGAGTTG CCTGAATATC ATACAGAGGA AAATGATAGC 
CCCATAGACG TCCAGAAAGA ACCCATTAAG GAGGTGCAAA GCGATGAAAA GGAACTCCAG 
AGGGAACATA TGAGTGAAGA AGAACAGAAA ATGAAAAGTA CAAATAAAAC AGCAGCTCCA 
GTAAGAAAAT CTCAAAGGTT GAAAAGAAGT AAGGAAGTTA AATTTGAAGA TGAAGAAAAG 
GAAGAGATAG AAGAAGATAA TACGAAAGAC GAAGAACAGA AAGAAAAGAA AGAGGAGATC 
CAGGAGCCAA AAATAACCCA CAATGAAGAA GTAGATAAGG AAAAAAACGA AAACGAAGAA 
GGTGATGATG AGCGTGAAAA ATCCACCTCT TATGAAAATA CCAATGGCTC TGAAAGCGAA 
GGAGTTGACG AAGGAGTTGA CGAAGAACTG GGATACGAGT CTGAACGTGA GGCAGAAGGT 
AAAGGAAAAC AAATTGAATC AGAAGGGGGC AACTTAAAGA AAAAAACGGA AAACAAAAAA 
GGAGATGATC AACAGGATGA TACCAAAAAG GATTCTAAGG ACAAAAATGA ACCATTAGCA 
AAGAGAACTA GGCATTCATC ATCCACAGGT AATACTAGCA ATGAAACCTC GCCGAAAAGG 
AAAAGGCGGA AGGCAGGCTC TCGTAAAAAT AGCCCTCCTG CCACAAGAGT TTCCAGCAGG 
CTACGAAATA AAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_014263.1
CDS	1..1278
Translation	MVVHWTIVDEIRLLRWASEFKPAGIHKHFHMFCIVERMNSPDKYPVTLLQKETMKLGKVFTAKDIWDKLSQSYNLEKIDEMENTYSLEATTESSRNGNGNGDDAEIHEETLLELNNRIRVRKQDFTLPWEEYGELILENARKSPNSNEEYPRVEDMNEKDSTIPKESPSTDLKNDNNKQEKNATIKVKELPEYHTEENDSPIDVQKEPIKEVQSDEKELQREHMSEEEQKMKSTNKTAAPVRKSQRLKRSKEVKFEDEEKEEIEEDNTKDEEQKEKKEEIQEPKITHNEEVDKEKNENEEGDDEREKSTSYENTNGSESEGVDEGVDEELGYESEREAEGKGKQIESEGGNLKKKTENKKGDDQQDDTKKDSKDKNEPLAKRTRHSSSTGNTSNETSPKRKRRKAGSRKNSPPATRVSSRLRNKK

Target ORF information:

RefSeq Version	NM_001182974.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Eaf7p (EAF7), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182974.1

ORF Insert Sequence:

ATGGTAGTAC ATTGGACAAT TGTGGATGAA ATACGACTTC TTAGATGGGC ATCTGAATTC 
AAACCGGCCG GAATTCATAA GCATTTCCAC ATGTTTTGCA TAGTGGAAAG AATGAATTCG 
CCAGATAAGT ATCCAGTGAC GCTATTACAG AAAGAAACCA TGAAGTTGGG GAAGGTATTT 
ACTGCAAAAG ACATATGGGA TAAGTTGAGC CAATCTTACA ATTTAGAGAA AATTGATGAA 
ATGGAGAATA CATATTCCTT GGAAGCCACT ACAGAGAGTT CACGGAATGG CAATGGAAAT 
GGTGACGATG CAGAAATTCA TGAAGAAACT TTACTTGAAT TGAATAATCG AATAAGAGTA 
CGCAAGCAAG ATTTTACATT ACCTTGGGAG GAATACGGTG AGTTGATCTT AGAAAATGCA 
AGAAAAAGTC CAAACTCTAA CGAAGAGTAT CCTCGAGTTG AAGATATGAA TGAGAAAGAT 
AGTACAATTC CAAAGGAAAG TCCTTCTACA GACCTAAAGA ACGACAACAA TAAACAGGAA 
AAAAATGCGA CAATTAAAGT GAAAGAGTTG CCTGAATATC ATACAGAGGA AAATGATAGC 
CCCATAGACG TCCAGAAAGA ACCCATTAAG GAGGTGCAAA GCGATGAAAA GGAACTCCAG 
AGGGAACATA TGAGTGAAGA AGAACAGAAA ATGAAAAGTA CAAATAAAAC AGCAGCTCCA 
GTAAGAAAAT CTCAAAGGTT GAAAAGAAGT AAGGAAGTTA AATTTGAAGA TGAAGAAAAG 
GAAGAGATAG AAGAAGATAA TACGAAAGAC GAAGAACAGA AAGAAAAGAA AGAGGAGATC 
CAGGAGCCAA AAATAACCCA CAATGAAGAA GTAGATAAGG AAAAAAACGA AAACGAAGAA 
GGTGATGATG AGCGTGAAAA ATCCACCTCT TATGAAAATA CCAATGGCTC TGAAAGCGAA 
GGAGTTGACG AAGGAGTTGA CGAAGAACTG GGATACGAGT CTGAACGTGA GGCAGAAGGT 
AAAGGAAAAC AAATTGAATC AGAAGGGGGC AACTTAAAGA AAAAAACGGA AAACAAAAAA 
GGAGATGATC AACAGGATGA TACCAAAAAG GATTCTAAGG ACAAAAATGA ACCATTAGCA 
AAGAGAACTA GGCATTCATC ATCCACAGGT AATACTAGCA ATGAAACCTC GCCGAAAAGG 
AAAAGGCGGA AGGCAGGCTC TCGTAAAAAT AGCCCTCCTG CCACAAGAGT TTCCAGCAGG 
CTACGAAATA AAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Nature387(6632 Suppl)93-8(1997 May)
Philippsen P,Kleine K,P?hlmann R,D?sterh?ft A,Hamberg K,Hegemann JH,Obermaier B,Urrestarazu LA,Aert R,Albermann K,Altmann R,Andr? B,Baladron V,Ballesta JP,B?cam AM,Beinhauer J,Boskovic J,Buitrago MJ,Bussereau F,Coster F,Crouzet M,D'Angelo M,Dal Pero F,De Antoni A,Del Rey F,Doignon F,Domdey H,Dubois E,Fiedler T,Fleig U,Floeth M,Fritz C,Gaillardin C,Garcia-Cantalejo JM,Glansdorff NN,Goffeau A,Gueldener U,Herbert C,Heumann K,Heuss-Neitzel D,Hilbert H,Hinni K,Iraqui Houssaini I,Jacquet M,Jimenez A,Jonniaux JL,Karpfinger L,Lanfranchi G,Lepingle A,Levesque H,Lyck R,Maftahi M,Mallet L,Maurer KC,Messenguy F,Mewes HW,M?sti D,Nasr F,Nicaud JM,Niedenthal RK,Pandolfo D,Pi?rard A,Piravandi E,Planta RJ,Pohl TM,Purnelle B,Rebischung C,Remacha M,Revuelta JL,Rinke M,Saiz JE,Sartorello F,Scherens B,Sen-Gupta M,Soler-Mira A,Urbanus JH,Valle G,Van Dyck L,Verhasselt P,Vierendeels F,Vissers S,Voet M,Volckaert G,Wach A,Wambutt R,Wedler H,Zollner A,Hani J

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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EAF7 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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