BNI5 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	BNI5
Entrez Gene ID	855555
Full Name	Bni5p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001183004.1	NP_014233.1	Bni5p

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Philippsen P, Kleine K, P?hlmann R, D?sterh?ft A, Hamberg K, Hegemann JH, Obermaier B, Urrestarazu LA, Aert R, Albermann K, Altmann R, Andr? B, Baladron V, Ballesta JP, B?cam AM, Beinhauer J, Boskovic J, Buitrago MJ, Bussereau F, Coster F, Crouzet M, D'Angelo M, Dal Pero F, De Antoni A, Del Rey F, Doignon F, Domdey H, Dubois E, Fiedler T, Fleig U, Floeth M, Fritz C, Gaillardin C, Garcia-Cantalejo JM, Glansdorff NN, Goffeau A, Gueldener U, Herbert C, Heumann K, Heuss-Neitzel D, Hilbert H, Hinni K, Iraqui Houssaini I, Jacquet M, Jimenez A, Jonniaux JL, Karpfinger L, Lanfranchi G, Lepingle A, Levesque H, Lyck R, Maftahi M, Mallet L, Maurer KC, Messenguy F, Mewes HW, M?sti D, Nasr F, Nicaud JM, Niedenthal RK, Pandolfo D, Pi?rard A, Piravandi E, Planta RJ, Pohl TM, Purnelle B, Rebischung C, Remacha M, Revuelta JL, Rinke M, Saiz JE, Sartorello F, Scherens B, Sen-Gupta M, Soler-Mira A, Urbanus JH, Valle G, Van Dyck L, Verhasselt P, Vierendeels F, Vissers S, Voet M, Volckaert G, Wach A, Wambutt R, Wedler H, Zollner A, Hani J
Nature387(6632 Suppl)93-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The authors found that the interaction of Bni5 with the terminal subunit (Cdc11) at the junctions between adjacent hetero-octamers in paired filaments is highly cooperative.
Title: Effects of Bni5 Binding on Septin Filament Organization.

Bni5 phosphorylation is important for interaction with septin component Cdc11 and for timely delocalization from septin filament at late mitosis.
Title: Phosphorylation-dependent septin interaction of Bni5 is important for cytokinesis.

These results suggest that bud morphogenesis and mitotic entry are positively regulated by phosphorylation-dependent function of Bni5 which is under the control of Swe1 morphogenesis pathway.
Title: Requirement of Bni5 phosphorylation for bud morphogenesis in Saccharomyces cerevisiae.

The following BNI5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BNI5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04472	NM_001183004.1 Latest version!	Saccharomyces cerevisiae S288C Bni5p (BNI5), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BNI5 ( NM_001183004.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_014233.1 Saccharomyces cerevisiae S288C Bni5p (BNI5), partial mRNA.

Price & Availability↑

CloneID	OSi04472
Clone ID Related Accession (Same CDS sequence)	NM_001183004.1
Accession Version	NM_001183004.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1347bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Bni5p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001146). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGGCTTGG ACCAGGACAA GATAAAGAAG AGGCTTTCTC AAATAGAGAT AGATATCAAC 
CAGATGAACC AGATGATTGA CGAAAATTTA CAATTAGTTG AACCTGCGGA GGACGAAGCT 
GTCGAAGATA ATGTCAAGGA TACGGGTGTG GTCGACGCTG TAAAGGTGGC AGAAACGGCT 
CTTTTTAGTG GCAATGATGG AGCGGATAGT AACCCAGGAG ATTCAGCTCA GGTTGAAGAG 
CACAAAACCG CACAGGTCCA TATTCCCACT GAGAATGAAG CGAATAAGAG TACGGATGAC 
CCATCCCAGC TATCGGTTAC TCAACCTTTT ATAGCGAAAG AGCAGATAAC TCACACCGCC 
ATTGCTATTG GTGACTCCTA TAATTCATTT GTCGCAAATT CCGCTGGAAA TGAAAAGGCA 
AAGGACTCCT GCACTGAGAA CAAAGAAGAT GGTACAGTCA ATATAGATCA AAACAGGGGT 
GAAGCTGATG TTGAAATTAT CGAAAATAAT GATGATGAAT GGGAGGATGA AAAATCGGAT 
GTTGAAGAGG GGCGAGTAGA TAAGGGAACA GAGGAAAACA GTGAGATTGA ATCTTTCAAA 
TCACCCATGC CCCAGAATAA CACCTTGGGC GGTGAGAACA AGTTAGACGC TGAACTTGTA 
TTAGACAAAT TTTCTTCCGC TAATAAGGAT CTAGATATAC AACCGCAAAC CATAGTTGTA 
GGAGGTGACA ATGAATACAA CCATGAGAGC AGCCGTTTAG CTGATCAAAC ACCCCATGAC 
GATAATTCAG AGAATTGTCC CAATCGCTCT GGAGGAAGCA CTCCCTTAGA TTCTCAAACT 
AAAATTTTTA TCCCTAAAAA GAATTCTAAA GAAGATGGTA CCAATATAAA TCACTTTAAT 
TCAGATGGTG ATGGACAGAA AAAAATGGCG AATTTCGAGA CACGACGCCC TACAAATCCG 
TTCAGAGTTA TCTCGGTGAG CAGCAATTCC AATTCAAGAA ACGGAAGTCG TAAATCTTCA 
CTAAATAAGT ATGATTCGCC AGTCTCCTCT CCTATCACAT CAGCGTCTGA GCTGGGCAGT 
ATTGCCAAGC TGGAAAAAAG ACATGATTAT TTATCCATGA AATGCATTAA GTTACAAAAG 
GAAATTGACT ATCTAAACAA GATGAATGCA CAGGGTTCTT TATCTATGGA AGACGGAAAG 
AGACTACATA GAGCCGTAGT GAAGCTTCAA GAATATCTTG ACAAGAAAAC GAAGGAGAAA 
TATGAGGTCG GAGTGTTATT AAGTAGACAT CTGAGAAAGC AAATTGATCG TGGCGAAAAT 
GGCCAATTTT GGATTGGAAC TAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_014233.1
CDS	1..1347
Translation	MGLDQDKIKKRLSQIEIDINQMNQMIDENLQLVEPAEDEAVEDNVKDTGVVDAVKVAETALFSGNDGADSNPGDSAQVEEHKTAQVHIPTENEANKSTDDPSQLSVTQPFIAKEQITHTAIAIGDSYNSFVANSAGNEKAKDSCTENKEDGTVNIDQNRGEADVEIIENNDDEWEDEKSDVEEGRVDKGTEENSEIESFKSPMPQNNTLGGENKLDAELVLDKFSSANKDLDIQPQTIVVGGDNEYNHESSRLADQTPHDDNSENCPNRSGGSTPLDSQTKIFIPKKNSKEDGTNINHFNSDGDGQKKMANFETRRPTNPFRVISVSSNSNSRNGSRKSSLNKYDSPVSSPITSASELGSIAKLEKRHDYLSMKCIKLQKEIDYLNKMNAQGSLSMEDGKRLHRAVVKLQEYLDKKTKEKYEVGVLLSRHLRKQIDRGENGQFWIGTK

Target ORF information:

RefSeq Version	NM_001183004.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Bni5p (BNI5), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001183004.1

ORF Insert Sequence:

ATGGGCTTGG ACCAGGACAA GATAAAGAAG AGGCTTTCTC AAATAGAGAT AGATATCAAC 
CAGATGAACC AGATGATTGA CGAAAATTTA CAATTAGTTG AACCTGCGGA GGACGAAGCT 
GTCGAAGATA ATGTCAAGGA TACGGGTGTG GTCGACGCTG TAAAGGTGGC AGAAACGGCT 
CTTTTTAGTG GCAATGATGG AGCGGATAGT AACCCAGGAG ATTCAGCTCA GGTTGAAGAG 
CACAAAACCG CACAGGTCCA TATTCCCACT GAGAATGAAG CGAATAAGAG TACGGATGAC 
CCATCCCAGC TATCGGTTAC TCAACCTTTT ATAGCGAAAG AGCAGATAAC TCACACCGCC 
ATTGCTATTG GTGACTCCTA TAATTCATTT GTCGCAAATT CCGCTGGAAA TGAAAAGGCA 
AAGGACTCCT GCACTGAGAA CAAAGAAGAT GGTACAGTCA ATATAGATCA AAACAGGGGT 
GAAGCTGATG TTGAAATTAT CGAAAATAAT GATGATGAAT GGGAGGATGA AAAATCGGAT 
GTTGAAGAGG GGCGAGTAGA TAAGGGAACA GAGGAAAACA GTGAGATTGA ATCTTTCAAA 
TCACCCATGC CCCAGAATAA CACCTTGGGC GGTGAGAACA AGTTAGACGC TGAACTTGTA 
TTAGACAAAT TTTCTTCCGC TAATAAGGAT CTAGATATAC AACCGCAAAC CATAGTTGTA 
GGAGGTGACA ATGAATACAA CCATGAGAGC AGCCGTTTAG CTGATCAAAC ACCCCATGAC 
GATAATTCAG AGAATTGTCC CAATCGCTCT GGAGGAAGCA CTCCCTTAGA TTCTCAAACT 
AAAATTTTTA TCCCTAAAAA GAATTCTAAA GAAGATGGTA CCAATATAAA TCACTTTAAT 
TCAGATGGTG ATGGACAGAA AAAAATGGCG AATTTCGAGA CACGACGCCC TACAAATCCG 
TTCAGAGTTA TCTCGGTGAG CAGCAATTCC AATTCAAGAA ACGGAAGTCG TAAATCTTCA 
CTAAATAAGT ATGATTCGCC AGTCTCCTCT CCTATCACAT CAGCGTCTGA GCTGGGCAGT 
ATTGCCAAGC TGGAAAAAAG ACATGATTAT TTATCCATGA AATGCATTAA GTTACAAAAG 
GAAATTGACT ATCTAAACAA GATGAATGCA CAGGGTTCTT TATCTATGGA AGACGGAAAG 
AGACTACATA GAGCCGTAGT GAAGCTTCAA GAATATCTTG ACAAGAAAAC GAAGGAGAAA 
TATGAGGTCG GAGTGTTATT AAGTAGACAT CTGAGAAAGC AAATTGATCG TGGCGAAAAT 
GGCCAATTTT GGATTGGAAC TAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Nature387(6632 Suppl)93-8(1997 May)
Philippsen P,Kleine K,P?hlmann R,D?sterh?ft A,Hamberg K,Hegemann JH,Obermaier B,Urrestarazu LA,Aert R,Albermann K,Altmann R,Andr? B,Baladron V,Ballesta JP,B?cam AM,Beinhauer J,Boskovic J,Buitrago MJ,Bussereau F,Coster F,Crouzet M,D'Angelo M,Dal Pero F,De Antoni A,Del Rey F,Doignon F,Domdey H,Dubois E,Fiedler T,Fleig U,Floeth M,Fritz C,Gaillardin C,Garcia-Cantalejo JM,Glansdorff NN,Goffeau A,Gueldener U,Herbert C,Heumann K,Heuss-Neitzel D,Hilbert H,Hinni K,Iraqui Houssaini I,Jacquet M,Jimenez A,Jonniaux JL,Karpfinger L,Lanfranchi G,Lepingle A,Levesque H,Lyck R,Maftahi M,Mallet L,Maurer KC,Messenguy F,Mewes HW,M?sti D,Nasr F,Nicaud JM,Niedenthal RK,Pandolfo D,Pi?rard A,Piravandi E,Planta RJ,Pohl TM,Purnelle B,Rebischung C,Remacha M,Revuelta JL,Rinke M,Saiz JE,Sartorello F,Scherens B,Sen-Gupta M,Soler-Mira A,Urbanus JH,Valle G,Van Dyck L,Verhasselt P,Vierendeels F,Vissers S,Voet M,Volckaert G,Wach A,Wambutt R,Wedler H,Zollner A,Hani J

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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BNI5 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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