UBP10 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	UBP10
Entrez Gene ID	855535
Full Name	ubiquitin-specific protease UBP10
Synonyms	DOT4
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001183024.2	NP_014213.2	ubiquitin-specific protease UBP10

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	UBP10	NP_014213.2
Kluyveromyces lactis	KLLA0D08459g	XP_453439.1
Eremothecium gossypii	AGOS_ABL145W	NP_982802.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Philippsen P, Kleine K, P?hlmann R, D?sterh?ft A, Hamberg K, Hegemann JH, Obermaier B, Urrestarazu LA, Aert R, Albermann K, Altmann R, Andr? B, Baladron V, Ballesta JP, B?cam AM, Beinhauer J, Boskovic J, Buitrago MJ, Bussereau F, Coster F, Crouzet M, D'Angelo M, Dal Pero F, De Antoni A, Del Rey F, Doignon F, Domdey H, Dubois E, Fiedler T, Fleig U, Floeth M, Fritz C, Gaillardin C, Garcia-Cantalejo JM, Glansdorff NN, Goffeau A, Gueldener U, Herbert C, Heumann K, Heuss-Neitzel D, Hilbert H, Hinni K, Iraqui Houssaini I, Jacquet M, Jimenez A, Jonniaux JL, Karpfinger L, Lanfranchi G, Lepingle A, Levesque H, Lyck R, Maftahi M, Mallet L, Maurer KC, Messenguy F, Mewes HW, M?sti D, Nasr F, Nicaud JM, Niedenthal RK, Pandolfo D, Pi?rard A, Piravandi E, Planta RJ, Pohl TM, Purnelle B, Rebischung C, Remacha M, Revuelta JL, Rinke M, Saiz JE, Sartorello F, Scherens B, Sen-Gupta M, Soler-Mira A, Urbanus JH, Valle G, Van Dyck L, Verhasselt P, Vierendeels F, Vissers S, Voet M, Volckaert G, Wach A, Wambutt R, Wedler H, Zollner A, Hani J
Nature387(6632 Suppl)93-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

show that Ubp10 possesses multiple, distinct binding modules within its IDRs that are necessary and sufficient for directing protein interactions important for Ubp10's known roles in gene silencing and ribosome biogenesis
Title: A Conserved Deubiquitinating Enzyme Uses Intrinsically Disordered Regions to Scaffold Multiple Protein Interaction Sites.

In this work we present clear evidence indicating that Ubp10 controls PCNA deubiquitylation in S. cerevisiae
Title: Reversal of PCNA ubiquitylation by Ubp10 in Saccharomyces cerevisiae.

The following UBP10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBP10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04457	NM_001183024.2 Latest version!	Saccharomyces cerevisiae S288C ubiquitin-specific protease UBP10 (UBP10), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UBP10 ( NM_001183024.2 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_014213.2 Saccharomyces cerevisiae S288C ubiquitin-specific protease UBP10 (UBP10), partial mRNA.

Price & Availability↑

CloneID	OSi04457
Clone ID Related Accession (Same CDS sequence)	NM_001183024.2
Accession Version	NM_001183024.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2379bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	ubiquitin-specific protease UBP10
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001146). On Apr 26, 2011 this sequence version replaced NM_001183024.1. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGACCACTC AAGAATCGAT CAAACCTTTG GTAGATAGAA TCCTATCAAA TCCCCTGCAG 
TTCAATGCTG CAATGATCTC TAACAAATCG AATAATAATG ATACTTCCGC CGCGCCGGAA 
AATAGCTCGT ATATTGTGAT AGGAAAACAG CATAATAACA ATAGTAATAG CACAGCTATT 
GCTGCAACGG CCGAATCCAA GCAAATAAAA GAAAATAACT TGATAGACAG GCCAAACGGA 
AAGAAAACCA ACACTGTTCC TAAATCTATG GCTGAAGCTT TATTGTTGTA TACTTCTAAA 
AATGATAAAG ATGCTGCAGA TGCTACTGGT GCCAAGAAGT CAGCGGAGCT TTCTACGGAG 
CTTTCTACGG AGCCTCCTTC CTCTTCTTCG GAAGATGACA AAGTAGGAAA AGAGGAAGAG 
GAAGAGGGTG AAATATTTCA TGAGGCAAGA GACTATGTAG AACCCCGAAA AGCTAGTTTG 
AAGGAACGCG ACAACGCAGA TAAGGGCGAT GGTGAAGACA TCGGCGAAGA CATCGGTGAA 
GACATCGGTG AAGACATCGG TGAAGACATT GGTGAAGACA TTGGTGAAAA CTTGGGTTCT 
CCATTAGCAA CCATTGATGA TTCATCTAAT GAGAATGAAA AGGAAAAAAG AAAGGAACTG 
TCTACAAGCA TTAGCAGTGA TGACGAAATA GAAGACGACG AGGATGAGGA TGACATGGAT 
TATGATTCTA GTGCTATGGA AAAAGAGCTC CCTGAAGAAG AGGAGAACGA TTCCAGCTCC 
AAAATTTCTG AAGGCGAAAA AAAGAGTTTA TATCAAGATT TAATGGAAAA TAGTACAGTG 
GAAGTAAATC GGTACGAACC AGTAAACAAC ACCAAAGAAA ATGGAAACAG GAATCCAAAG 
GGAGAGGAGG AGGAAGAAGA GGAAGAAGAG CTGAAACATA AATCTAGGTC AATCACCCCT 
CCGGTTACAA TATCAAATCT ATCAAACTTT TACCAATTCA ATGAAAATAT CAATGATCGT 
GGTTCTTTAA ACTCTACTAG AATTGTTAAA AATTGGGGCG ACAAATTCAC CAATTTGAAG 
CCTCGTGGCC TTTTGAATCA TGGTGTTACT TGTTACACAA ATGCTGCTGT ACAGGCTATG 
TTACACATTC CTTCGATACA ACATTATCTT TTTGATATAC TAATGGGGAA ATACGATAGC 
ACCATCTCAA AAAATTCCGT TTCCTATACT TTAGCTGAAA CAAGTAAAAA AATGTGGTTA 
CCGGTCTCAA AAAACCCTAG AAAGAACGTT TCAGCTTCCT ACATTAATCC AAAACATTTG 
ATTTCCAGAT TGGATGACAT TAATTGTATG ATGAGCGAAT GGCAGCAGGA AGATTCACAT 
GAGTACTTCA TGTCTCTGAT GTCAAGATTA CAGGAAGATT CTGTTCCCAA GGGTCATAAA 
CTTATAGAAT CGATAATATA TGACATATTC GGTGGTCTTT TAAAGCAGAT CGTTACTTGC 
AAATCTTGTG GCAGTATATC TAAAACAGAA CAACCATTTT ACGATTTATC GTTGCACTTG 
AAAGGGAAGA AAAAACTTGA TCCAAATTCT GACCTGTCGA GTGATAGCAT TAACGGCACT 
TCAGCCACCA CTTCTACCAC TACCTCCAAT GCTGCCACAA AACCATCTCT TTCATCCTCT 
TCATCTGTCA ATTTAAACAA TGGCTCACCA TTTGCCGCTG CCAGTGATTT AAGTTCAGCC 
AACCGCAGAT TTTCTATTGA AAAATCAATT AAAGATTTCT TCAATCCCGA ATTAATCAAG 
GTTGACAAGG AGCAAAAGGG TTACGTTTGT GAGAAGTGTC ACAAGACCAC GAACGCCGTG 
AAGCATAGTT CAATATTAAG GGCTCCTGAA ACTTTACTTG TGCATCTGAA AAAATTCAGA 
TTCAATGGCA CGTCCTCATC AAAAATGAAG CAAGCTGTTT CTTATCCTAT GTTTTTAGAT 
TTGACGGAAT ATTGTGAGAG TAAAGAGCTA CCTGTCAAAT ACCAACTATT AAGCGTGGTG 
GTTCATGAGG GCCGCTCCCT TTCTTCAGGT CACTACATTG CCCACTGCAA GCAACCAGAC 
GGTAGCTGGG CCACTTACGA CGACGAGTAT ATTAATATAA TATCTGAAAG GGACGTTTTA 
AAGGAACCCA ACGCATATTA TCTCCTATAC ACGAGGCTAA CTCCAAAATC GGTTCCATTG 
CCATTGGCGA AATCTGCCAT GGCCACTGGT AATGTTACCT CTAAATCCAA ACAGGAACAG 
GCTGTTAACG AACCAAATAA CCGCCCATTG AAGATTAATA GCAAGAAAAA TAACAGAAAA 
AAATGGAAAA AAAATAAAAA AAGGAAGTTC ACCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_014213.2
CDS	1..2379
Translation	MTTQESIKPLVDRILSNPLQFNAAMISNKSNNNDTSAAPENSSYIVIGKQHNNNSNSTAIAATAESKQIKENNLIDRPNGKKTNTVPKSMAEALLLYTSKNDKDAADATGAKKSAELSTELSTEPPSSSSEDDKVGKEEEEEGEIFHEARDYVEPRKASLKERDNADKGDGEDIGEDIGEDIGEDIGEDIGEDIGENLGSPLATIDDSSNENEKEKRKELSTSISSDDEIEDDEDEDDMDYDSSAMEKELPEEEENDSSSKISEGEKKSLYQDLMENSTVEVNRYEPVNNTKENGNRNPKGEEEEEEEEELKHKSRSITPPVTISNLSNFYQFNENINDRGSLNSTRIVKNWGDKFTNLKPRGLLNHGVTCYTNAAVQAMLHIPSIQHYLFDILMGKYDSTISKNSVSYTLAETSKKMWLPVSKNPRKNVSASYINPKHLISRLDDINCMMSEWQQEDSHEYFMSLMSRLQEDSVPKGHKLIESIIYDIFGGLLKQIVTCKSCGSISKTEQPFYDLSLHLKGKKKLDPNSDLSSDSINGTSATTSTTTSNAATKPSLSSSSSVNLNNGSPFAAASDLSSANRRFSIEKSIKDFFNPELIKVDKEQKGYVCEKCHKTTNAVKHSSILRAPETLLVHLKKFRFNGTSSSKMKQAVSYPMFLDLTEYCESKELPVKYQLLSVVVHEGRSLSSGHYIAHCKQPDGSWATYDDEYINIISERDVLKEPNAYYLLYTRLTPKSVPLPLAKSAMATGNVTSKSKQEQAVNEPNNRPLKINSKKNNRKKWKKNKKRKFTK

Target ORF information:

RefSeq Version	NM_001183024.2
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C ubiquitin-specific protease UBP10 (UBP10), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001183024.2

ORF Insert Sequence:

ATGACCACTC AAGAATCGAT CAAACCTTTG GTAGATAGAA TCCTATCAAA TCCCCTGCAG 
TTCAATGCTG CAATGATCTC TAACAAATCG AATAATAATG ATACTTCCGC CGCGCCGGAA 
AATAGCTCGT ATATTGTGAT AGGAAAACAG CATAATAACA ATAGTAATAG CACAGCTATT 
GCTGCAACGG CCGAATCCAA GCAAATAAAA GAAAATAACT TGATAGACAG GCCAAACGGA 
AAGAAAACCA ACACTGTTCC TAAATCTATG GCTGAAGCTT TATTGTTGTA TACTTCTAAA 
AATGATAAAG ATGCTGCAGA TGCTACTGGT GCCAAGAAGT CAGCGGAGCT TTCTACGGAG 
CTTTCTACGG AGCCTCCTTC CTCTTCTTCG GAAGATGACA AAGTAGGAAA AGAGGAAGAG 
GAAGAGGGTG AAATATTTCA TGAGGCAAGA GACTATGTAG AACCCCGAAA AGCTAGTTTG 
AAGGAACGCG ACAACGCAGA TAAGGGCGAT GGTGAAGACA TCGGCGAAGA CATCGGTGAA 
GACATCGGTG AAGACATCGG TGAAGACATT GGTGAAGACA TTGGTGAAAA CTTGGGTTCT 
CCATTAGCAA CCATTGATGA TTCATCTAAT GAGAATGAAA AGGAAAAAAG AAAGGAACTG 
TCTACAAGCA TTAGCAGTGA TGACGAAATA GAAGACGACG AGGATGAGGA TGACATGGAT 
TATGATTCTA GTGCTATGGA AAAAGAGCTC CCTGAAGAAG AGGAGAACGA TTCCAGCTCC 
AAAATTTCTG AAGGCGAAAA AAAGAGTTTA TATCAAGATT TAATGGAAAA TAGTACAGTG 
GAAGTAAATC GGTACGAACC AGTAAACAAC ACCAAAGAAA ATGGAAACAG GAATCCAAAG 
GGAGAGGAGG AGGAAGAAGA GGAAGAAGAG CTGAAACATA AATCTAGGTC AATCACCCCT 
CCGGTTACAA TATCAAATCT ATCAAACTTT TACCAATTCA ATGAAAATAT CAATGATCGT 
GGTTCTTTAA ACTCTACTAG AATTGTTAAA AATTGGGGCG ACAAATTCAC CAATTTGAAG 
CCTCGTGGCC TTTTGAATCA TGGTGTTACT TGTTACACAA ATGCTGCTGT ACAGGCTATG 
TTACACATTC CTTCGATACA ACATTATCTT TTTGATATAC TAATGGGGAA ATACGATAGC 
ACCATCTCAA AAAATTCCGT TTCCTATACT TTAGCTGAAA CAAGTAAAAA AATGTGGTTA 
CCGGTCTCAA AAAACCCTAG AAAGAACGTT TCAGCTTCCT ACATTAATCC AAAACATTTG 
ATTTCCAGAT TGGATGACAT TAATTGTATG ATGAGCGAAT GGCAGCAGGA AGATTCACAT 
GAGTACTTCA TGTCTCTGAT GTCAAGATTA CAGGAAGATT CTGTTCCCAA GGGTCATAAA 
CTTATAGAAT CGATAATATA TGACATATTC GGTGGTCTTT TAAAGCAGAT CGTTACTTGC 
AAATCTTGTG GCAGTATATC TAAAACAGAA CAACCATTTT ACGATTTATC GTTGCACTTG 
AAAGGGAAGA AAAAACTTGA TCCAAATTCT GACCTGTCGA GTGATAGCAT TAACGGCACT 
TCAGCCACCA CTTCTACCAC TACCTCCAAT GCTGCCACAA AACCATCTCT TTCATCCTCT 
TCATCTGTCA ATTTAAACAA TGGCTCACCA TTTGCCGCTG CCAGTGATTT AAGTTCAGCC 
AACCGCAGAT TTTCTATTGA AAAATCAATT AAAGATTTCT TCAATCCCGA ATTAATCAAG 
GTTGACAAGG AGCAAAAGGG TTACGTTTGT GAGAAGTGTC ACAAGACCAC GAACGCCGTG 
AAGCATAGTT CAATATTAAG GGCTCCTGAA ACTTTACTTG TGCATCTGAA AAAATTCAGA 
TTCAATGGCA CGTCCTCATC AAAAATGAAG CAAGCTGTTT CTTATCCTAT GTTTTTAGAT 
TTGACGGAAT ATTGTGAGAG TAAAGAGCTA CCTGTCAAAT ACCAACTATT AAGCGTGGTG 
GTTCATGAGG GCCGCTCCCT TTCTTCAGGT CACTACATTG CCCACTGCAA GCAACCAGAC 
GGTAGCTGGG CCACTTACGA CGACGAGTAT ATTAATATAA TATCTGAAAG GGACGTTTTA 
AAGGAACCCA ACGCATATTA TCTCCTATAC ACGAGGCTAA CTCCAAAATC GGTTCCATTG 
CCATTGGCGA AATCTGCCAT GGCCACTGGT AATGTTACCT CTAAATCCAA ACAGGAACAG 
GCTGTTAACG AACCAAATAA CCGCCCATTG AAGATTAATA GCAAGAAAAA TAACAGAAAA 
AAATGGAAAA AAAATAAAAA AAGGAAGTTC ACCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Nature387(6632 Suppl)93-8(1997 May)
Philippsen P,Kleine K,P?hlmann R,D?sterh?ft A,Hamberg K,Hegemann JH,Obermaier B,Urrestarazu LA,Aert R,Albermann K,Altmann R,Andr? B,Baladron V,Ballesta JP,B?cam AM,Beinhauer J,Boskovic J,Buitrago MJ,Bussereau F,Coster F,Crouzet M,D'Angelo M,Dal Pero F,De Antoni A,Del Rey F,Doignon F,Domdey H,Dubois E,Fiedler T,Fleig U,Floeth M,Fritz C,Gaillardin C,Garcia-Cantalejo JM,Glansdorff NN,Goffeau A,Gueldener U,Herbert C,Heumann K,Heuss-Neitzel D,Hilbert H,Hinni K,Iraqui Houssaini I,Jacquet M,Jimenez A,Jonniaux JL,Karpfinger L,Lanfranchi G,Lepingle A,Levesque H,Lyck R,Maftahi M,Mallet L,Maurer KC,Messenguy F,Mewes HW,M?sti D,Nasr F,Nicaud JM,Niedenthal RK,Pandolfo D,Pi?rard A,Piravandi E,Planta RJ,Pohl TM,Purnelle B,Rebischung C,Remacha M,Revuelta JL,Rinke M,Saiz JE,Sartorello F,Scherens B,Sen-Gupta M,Soler-Mira A,Urbanus JH,Valle G,Van Dyck L,Verhasselt P,Vierendeels F,Vissers S,Voet M,Volckaert G,Wach A,Wambutt R,Wedler H,Zollner A,Hani J

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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UBP10 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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