VID27 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	VID27
Entrez Gene ID	855509
Full Name	Vid27p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001183050.1	NP_014187.1	Vid27p

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	SPBC1685.14c	NP_595218.1
Magnaporthe oryzae (rice blast fungus)	MGG_03859	XP_003720032.1
Saccharomyces cerevisiae (baker's yeast)	VID27	NP_014187.1
Neurospora crassa	NCU09711	XP_958950.1
Eremothecium gossypii	AGOS_ABL168C	NP_982779.1
Kluyveromyces lactis	KLLA0D19162g	XP_453912.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Philippsen P, Kleine K, P?hlmann R, D?sterh?ft A, Hamberg K, Hegemann JH, Obermaier B, Urrestarazu LA, Aert R, Albermann K, Altmann R, Andr? B, Baladron V, Ballesta JP, B?cam AM, Beinhauer J, Boskovic J, Buitrago MJ, Bussereau F, Coster F, Crouzet M, D'Angelo M, Dal Pero F, De Antoni A, Del Rey F, Doignon F, Domdey H, Dubois E, Fiedler T, Fleig U, Floeth M, Fritz C, Gaillardin C, Garcia-Cantalejo JM, Glansdorff NN, Goffeau A, Gueldener U, Herbert C, Heumann K, Heuss-Neitzel D, Hilbert H, Hinni K, Iraqui Houssaini I, Jacquet M, Jimenez A, Jonniaux JL, Karpfinger L, Lanfranchi G, Lepingle A, Levesque H, Lyck R, Maftahi M, Mallet L, Maurer KC, Messenguy F, Mewes HW, M?sti D, Nasr F, Nicaud JM, Niedenthal RK, Pandolfo D, Pi?rard A, Piravandi E, Planta RJ, Pohl TM, Purnelle B, Rebischung C, Remacha M, Revuelta JL, Rinke M, Saiz JE, Sartorello F, Scherens B, Sen-Gupta M, Soler-Mira A, Urbanus JH, Valle G, Van Dyck L, Verhasselt P, Vierendeels F, Vissers S, Voet M, Volckaert G, Wach A, Wambutt R, Wedler H, Zollner A, Hani J
Nature387(6632 Suppl)93-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

inactivation of Vid27 (vacuolar import and degradation) prevents suppression of the doa4 phenotype by did3 mutation
Title: Inactivation of the VID27 gene prevents suppression of the doa4 degradation defect in doa4? did3? double mutant.

The following VID27 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VID27 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04434	NM_001183050.1 Latest version!	Saccharomyces cerevisiae S288C Vid27p (VID27), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VID27 ( NM_001183050.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_014187.1 Saccharomyces cerevisiae S288C Vid27p (VID27), partial mRNA.

Price & Availability↑

CloneID	OSi04434
Clone ID Related Accession (Same CDS sequence)	NM_001183050.1
Accession Version	NM_001183050.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2349bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Vid27p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001146). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAACATTT TGAAGAAGTT TATGGAGTCT GGTAACAAGC CAGAGCTCAT CACGATACCT 
TCTGGGCAGT TTAATCTTTT GAGGTCTAAG AATTCCCCTA AAGCTGCTTT AGAATGTATT 
TACAACAATG CAACTTTAAG CGTACGTAAA ATAGGCAAAT TTGATTATGA GTTGGCGGTT 
TATAGGGTAG AGGACGATAG TGAAGGAGGC ACGGGAGATG AGGCTGAGAA TTTTGAAGAC 
GACACCATCA GTGTTTTATC CACACAGTCT AAAAAGAAAG AAGAGGAGTG GAGCGTTGAA 
ATATCGGATA AAATCATGTT TCACAAAACA TGGGATAAAC AAGGAAACGT GGCGCTTGTG 
TGGGAGAACT TAAGAGGTGA TGAACAAGAT GAAAAGGTTC AATTTGTTGT TGCCGCTGAT 
GTGTCGTTTT CTGATGTTGA ACAGTTTATC CAAACTGTTT ACCGATGCCA ATTCGAAGTA 
AGGAATAAAA AATCCTCTCT CACTGCCTCA GCAGATGACT TAAAGGAAAT AGAGCATAGA 
TCAACCAGGC TCTTTGTTCA AGATGATGAT GACGAGTTAG ATTCAAGCTC AGATGACTTT 
CAAGATGCTA AAGACACCTC TTTTGAGCAC GAGAAGGAAA GTGAAATATT GGAAAGAACT 
CCTTCTCCAT TGAAAAAAGT TCCAGAAGGG GAATATTGTT GTTTAGTGAT GTCTAGCCTA 
TACATGTATG ATCCAATTCA AGAAAAATTT ATTCTGCAGG AGCCCGTGGT AAAGGTAGCA 
ATTATTGATA CAGGCAAGTA CGAATTTTGG CTAGCGATAG AAGGTAAAGA TAATCGTCTT 
GGGACTCAGG TCGCCCCAAA TATCAATCCT ACCTTTGAAT TAGCTACCGA TGCCTTTCTT 
TTCAACTATA CGCTACAAAA CATTACATTG TCTTATATGT TAAAATTTAA GGACTTAGAT 
AAGTGTATTC AATTCAGGTT TGCTTGGGTT AAATGTTTAT GGATGACTTT GAACAAAGAA 
ACGTGGACCG ACGTTCCTGA AAAGGAGAAA GATTATATTT TAGATTCATC ATCCGTGCCA 
TTGGAAAAGC AATTTGATGA CATTTTACAC ATAGATGACA GATCAAATGA GGAAAGAGAT 
AAAGAATCAA GCGAAAGTGA AAATGATAGC GAAGATGAGG ATGACGAAAA CGATCACTCC 
AAAAGAATCA TTTCATCTGA AGCCTTTGAA GAACCTAGGC GTGCGACTTC CAAAGGTAAT 
AGTTCATTGA CTGTCGCCTT TAGGAACAAC AGGTCATATG TTACAAGAGA TAACAGAATT 
GGTGTGTTCA AAACGGATGA TGAAGACGAT TCTCTAGAAT TTGTAGCTGC CATAAAGAAT 
ATTTCTAATT TAGGTGGTAA AAGCATTGAT CCTCATAAGC CAATGTTGTA TATGGAAGAT 
CGTAATTTAA TTTTGACTGA TGGCGAAAAT GAGAACAAAC TTTATAAGAT GGATATAGAA 
AGAGGAAAGG TGATTGAAGA ATGGTCTACT GGTGACAAGA ATGTTGTTCA ATATGGTCCA 
ACCAAAAAAT TCGATCAAAT GACGCCTGAA CAAACAATTG TTGGCGTTTC ACAGAAAGGT 
GTCTTTAAAA TTGATCCTAG AATTAACGGA AAAAATAAAA TAGCCGTCGA TGAATCAAAA 
GATTACGTGG GTAAATATAA CTTTAGCTCT ATTGGAACAA CAGAGAGTGG TTATATTGCT 
ATTGGGTCAG AAAAAGGTGA CATAAAACTA TATGATAGGC TCGGTATTAG GGCAAAAACT 
GCTATACCTT CACTAGGCCA AGCAATCAAG TTTATTACTA CCTCTGCTGA CGGTAAATGG 
TTATTGGCGA CTTGCGAGAG TACTCTGCTT CTTATGGATT TGAAAATTAA GGATGGAAAA 
AATGCTGGCA ACATTGGATT TCTGAAGTCG TTCCCAGCCA GTGAAAATGT TAAAACATAT 
GTATTAAAAA TACGACCAGA ACATTCGGCA TCAATCTTAA CGTACACAAA AAAACCAATC 
CGATTTACGA AGGCTTATTT TAATACCGGT ATAGGCCAGC AAGAACAAAC TATTGTTACA 
TCGACGGGTC CTTATGCGAT TTCTTGGTCA TTAAAGGGCA TTTTAAATCA AGATGGTAGT 
AATAATTATC CTTACAGAAT CCGTAGGTAC AATGCTGATG TTGTTGCTGA TAATTTTGAA 
TTTGGTTCAG ACAAAAAGGT TATCGTTGCC TTGAAAGACG ATGTCTCTCT ATCGAAAGTG 
AAATCCTTTA AACAGCCAAG CAAAGGAGTC TTGATGCCAA GTGCTTCCCT CCAAGATTTT 
TATGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_014187.1
CDS	1..2349
Translation	MNILKKFMESGNKPELITIPSGQFNLLRSKNSPKAALECIYNNATLSVRKIGKFDYELAVYRVEDDSEGGTGDEAENFEDDTISVLSTQSKKKEEEWSVEISDKIMFHKTWDKQGNVALVWENLRGDEQDEKVQFVVAADVSFSDVEQFIQTVYRCQFEVRNKKSSLTASADDLKEIEHRSTRLFVQDDDDELDSSSDDFQDAKDTSFEHEKESEILERTPSPLKKVPEGEYCCLVMSSLYMYDPIQEKFILQEPVVKVAIIDTGKYEFWLAIEGKDNRLGTQVAPNINPTFELATDAFLFNYTLQNITLSYMLKFKDLDKCIQFRFAWVKCLWMTLNKETWTDVPEKEKDYILDSSSVPLEKQFDDILHIDDRSNEERDKESSESENDSEDEDDENDHSKRIISSEAFEEPRRATSKGNSSLTVAFRNNRSYVTRDNRIGVFKTDDEDDSLEFVAAIKNISNLGGKSIDPHKPMLYMEDRNLILTDGENENKLYKMDIERGKVIEEWSTGDKNVVQYGPTKKFDQMTPEQTIVGVSQKGVFKIDPRINGKNKIAVDESKDYVGKYNFSSIGTTESGYIAIGSEKGDIKLYDRLGIRAKTAIPSLGQAIKFITTSADGKWLLATCESTLLLMDLKIKDGKNAGNIGFLKSFPASENVKTYVLKIRPEHSASILTYTKKPIRFTKAYFNTGIGQQEQTIVTSTGPYAISWSLKGILNQDGSNNYPYRIRRYNADVVADNFEFGSDKKVIVALKDDVSLSKVKSFKQPSKGVLMPSASLQDFYG

Target ORF information:

RefSeq Version	NM_001183050.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Vid27p (VID27), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001183050.1

ORF Insert Sequence:

ATGAACATTT TGAAGAAGTT TATGGAGTCT GGTAACAAGC CAGAGCTCAT CACGATACCT 
TCTGGGCAGT TTAATCTTTT GAGGTCTAAG AATTCCCCTA AAGCTGCTTT AGAATGTATT 
TACAACAATG CAACTTTAAG CGTACGTAAA ATAGGCAAAT TTGATTATGA GTTGGCGGTT 
TATAGGGTAG AGGACGATAG TGAAGGAGGC ACGGGAGATG AGGCTGAGAA TTTTGAAGAC 
GACACCATCA GTGTTTTATC CACACAGTCT AAAAAGAAAG AAGAGGAGTG GAGCGTTGAA 
ATATCGGATA AAATCATGTT TCACAAAACA TGGGATAAAC AAGGAAACGT GGCGCTTGTG 
TGGGAGAACT TAAGAGGTGA TGAACAAGAT GAAAAGGTTC AATTTGTTGT TGCCGCTGAT 
GTGTCGTTTT CTGATGTTGA ACAGTTTATC CAAACTGTTT ACCGATGCCA ATTCGAAGTA 
AGGAATAAAA AATCCTCTCT CACTGCCTCA GCAGATGACT TAAAGGAAAT AGAGCATAGA 
TCAACCAGGC TCTTTGTTCA AGATGATGAT GACGAGTTAG ATTCAAGCTC AGATGACTTT 
CAAGATGCTA AAGACACCTC TTTTGAGCAC GAGAAGGAAA GTGAAATATT GGAAAGAACT 
CCTTCTCCAT TGAAAAAAGT TCCAGAAGGG GAATATTGTT GTTTAGTGAT GTCTAGCCTA 
TACATGTATG ATCCAATTCA AGAAAAATTT ATTCTGCAGG AGCCCGTGGT AAAGGTAGCA 
ATTATTGATA CAGGCAAGTA CGAATTTTGG CTAGCGATAG AAGGTAAAGA TAATCGTCTT 
GGGACTCAGG TCGCCCCAAA TATCAATCCT ACCTTTGAAT TAGCTACCGA TGCCTTTCTT 
TTCAACTATA CGCTACAAAA CATTACATTG TCTTATATGT TAAAATTTAA GGACTTAGAT 
AAGTGTATTC AATTCAGGTT TGCTTGGGTT AAATGTTTAT GGATGACTTT GAACAAAGAA 
ACGTGGACCG ACGTTCCTGA AAAGGAGAAA GATTATATTT TAGATTCATC ATCCGTGCCA 
TTGGAAAAGC AATTTGATGA CATTTTACAC ATAGATGACA GATCAAATGA GGAAAGAGAT 
AAAGAATCAA GCGAAAGTGA AAATGATAGC GAAGATGAGG ATGACGAAAA CGATCACTCC 
AAAAGAATCA TTTCATCTGA AGCCTTTGAA GAACCTAGGC GTGCGACTTC CAAAGGTAAT 
AGTTCATTGA CTGTCGCCTT TAGGAACAAC AGGTCATATG TTACAAGAGA TAACAGAATT 
GGTGTGTTCA AAACGGATGA TGAAGACGAT TCTCTAGAAT TTGTAGCTGC CATAAAGAAT 
ATTTCTAATT TAGGTGGTAA AAGCATTGAT CCTCATAAGC CAATGTTGTA TATGGAAGAT 
CGTAATTTAA TTTTGACTGA TGGCGAAAAT GAGAACAAAC TTTATAAGAT GGATATAGAA 
AGAGGAAAGG TGATTGAAGA ATGGTCTACT GGTGACAAGA ATGTTGTTCA ATATGGTCCA 
ACCAAAAAAT TCGATCAAAT GACGCCTGAA CAAACAATTG TTGGCGTTTC ACAGAAAGGT 
GTCTTTAAAA TTGATCCTAG AATTAACGGA AAAAATAAAA TAGCCGTCGA TGAATCAAAA 
GATTACGTGG GTAAATATAA CTTTAGCTCT ATTGGAACAA CAGAGAGTGG TTATATTGCT 
ATTGGGTCAG AAAAAGGTGA CATAAAACTA TATGATAGGC TCGGTATTAG GGCAAAAACT 
GCTATACCTT CACTAGGCCA AGCAATCAAG TTTATTACTA CCTCTGCTGA CGGTAAATGG 
TTATTGGCGA CTTGCGAGAG TACTCTGCTT CTTATGGATT TGAAAATTAA GGATGGAAAA 
AATGCTGGCA ACATTGGATT TCTGAAGTCG TTCCCAGCCA GTGAAAATGT TAAAACATAT 
GTATTAAAAA TACGACCAGA ACATTCGGCA TCAATCTTAA CGTACACAAA AAAACCAATC 
CGATTTACGA AGGCTTATTT TAATACCGGT ATAGGCCAGC AAGAACAAAC TATTGTTACA 
TCGACGGGTC CTTATGCGAT TTCTTGGTCA TTAAAGGGCA TTTTAAATCA AGATGGTAGT 
AATAATTATC CTTACAGAAT CCGTAGGTAC AATGCTGATG TTGTTGCTGA TAATTTTGAA 
TTTGGTTCAG ACAAAAAGGT TATCGTTGCC TTGAAAGACG ATGTCTCTCT ATCGAAAGTG 
AAATCCTTTA AACAGCCAAG CAAAGGAGTC TTGATGCCAA GTGCTTCCCT CCAAGATTTT 
TATGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Nature387(6632 Suppl)93-8(1997 May)
Philippsen P,Kleine K,P?hlmann R,D?sterh?ft A,Hamberg K,Hegemann JH,Obermaier B,Urrestarazu LA,Aert R,Albermann K,Altmann R,Andr? B,Baladron V,Ballesta JP,B?cam AM,Beinhauer J,Boskovic J,Buitrago MJ,Bussereau F,Coster F,Crouzet M,D'Angelo M,Dal Pero F,De Antoni A,Del Rey F,Doignon F,Domdey H,Dubois E,Fiedler T,Fleig U,Floeth M,Fritz C,Gaillardin C,Garcia-Cantalejo JM,Glansdorff NN,Goffeau A,Gueldener U,Herbert C,Heumann K,Heuss-Neitzel D,Hilbert H,Hinni K,Iraqui Houssaini I,Jacquet M,Jimenez A,Jonniaux JL,Karpfinger L,Lanfranchi G,Lepingle A,Levesque H,Lyck R,Maftahi M,Mallet L,Maurer KC,Messenguy F,Mewes HW,M?sti D,Nasr F,Nicaud JM,Niedenthal RK,Pandolfo D,Pi?rard A,Piravandi E,Planta RJ,Pohl TM,Purnelle B,Rebischung C,Remacha M,Revuelta JL,Rinke M,Saiz JE,Sartorello F,Scherens B,Sen-Gupta M,Soler-Mira A,Urbanus JH,Valle G,Van Dyck L,Verhasselt P,Vierendeels F,Vissers S,Voet M,Volckaert G,Wach A,Wambutt R,Wedler H,Zollner A,Hani J

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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VID27 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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