BNI4 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	BNI4
Entrez Gene ID	855488
Full Name	Bni4p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001183071.1	NP_014166.1	Bni4p

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0D19723g	XP_453938.1
Saccharomyces cerevisiae (baker's yeast)	BNI4	NP_014166.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Philippsen P, Kleine K, P?hlmann R, D?sterh?ft A, Hamberg K, Hegemann JH, Obermaier B, Urrestarazu LA, Aert R, Albermann K, Altmann R, Andr? B, Baladron V, Ballesta JP, B?cam AM, Beinhauer J, Boskovic J, Buitrago MJ, Bussereau F, Coster F, Crouzet M, D'Angelo M, Dal Pero F, De Antoni A, Del Rey F, Doignon F, Domdey H, Dubois E, Fiedler T, Fleig U, Floeth M, Fritz C, Gaillardin C, Garcia-Cantalejo JM, Glansdorff NN, Goffeau A, Gueldener U, Herbert C, Heumann K, Heuss-Neitzel D, Hilbert H, Hinni K, Iraqui Houssaini I, Jacquet M, Jimenez A, Jonniaux JL, Karpfinger L, Lanfranchi G, Lepingle A, Levesque H, Lyck R, Maftahi M, Mallet L, Maurer KC, Messenguy F, Mewes HW, M?sti D, Nasr F, Nicaud JM, Niedenthal RK, Pandolfo D, Pi?rard A, Piravandi E, Planta RJ, Pohl TM, Purnelle B, Rebischung C, Remacha M, Revuelta JL, Rinke M, Saiz JE, Sartorello F, Scherens B, Sen-Gupta M, Soler-Mira A, Urbanus JH, Valle G, Van Dyck L, Verhasselt P, Vierendeels F, Vissers S, Voet M, Volckaert G, Wach A, Wambutt R, Wedler H, Zollner A, Hani J
Nature387(6632 Suppl)93-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results, together with a genetic correlation analysis, strongly support a functional model linking Slt2 MAP kinase and Pcl1, a Pho85 cyclin-dependent kinase, in septum assembly through Bni4.
Title: Phosphorylation of Bni4 by MAP kinases contributes to septum assembly during yeast cytokinesis.

Phosphorylation of Bni4 by Pcl-Pho85 is necessary for its localization to the bud neck, and the bud neck structure can be disrupted by overexpressing BNI4 in pcl1Deltapcl2Delta mutant cells.
Title: Regulation of cell polarity through phosphorylation of Bni4 by Pho85 G1 cyclin-dependent kinases in Saccharomyces cerevisiae.

the essential role of Bni4 is to target Glc7 to the neck and activate it toward substrates necessary for CSIII recruitment and synthesis of chitin at the bud neck
Title: Protein phosphatase type 1 directs chitin synthesis at the bud neck in Saccharomyces cerevisiae.

The following BNI4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BNI4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04415	NM_001183071.1 Latest version!	Saccharomyces cerevisiae S288C Bni4p (BNI4), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BNI4 ( NM_001183071.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_014166.1 Saccharomyces cerevisiae S288C Bni4p (BNI4), partial mRNA.

Price & Availability↑

CloneID	OSi04415
Clone ID Related Accession (Same CDS sequence)	NM_001183071.1
Accession Version	NM_001183071.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2679bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Bni4p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001146). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCGGATA GTATTTCAGA TTCAAAGTCC TCAGAACTTT TAAATTCTAC TTTTTATTCG 
TCGACGTCGA TAAATACACT TGATCATGCT AGAACCTTTC GAAATTCTCT GATATTGAAG 
GAGATATCAG ATCAAAGTCT AAATTCATCA ATAAAGCCAT GCGAATCCGT CCTTGATAGG 
GATGTTGAAA GCTCAGTTCT GCAGCGGTCG TTTGGAGAAA GTAATGCCCG AGACTCAGAA 
GTACAAACGG TAAATATGAC AACTTCCCCC TCATTAAGTG CATTGGCTGA TATTCTGAAC 
GAAAGATCCA AATATGCTGA CCAAAAAACT AGAAAGGCAC AAAATATTGA GTCTTCCATA 
ATAGAAGAAG AAGAAGAGGC CGAAGAGCAA AACAACTCGA TCAATTATCA CGAGGATATA 
ACTGGATCCA GGCTGTCTGT ACGAGAGGAA GCAAATGAAA ACTTGGCGAT GACCTCTCCA 
AACCTTATTG ACATTGACGG ATCCAATAGT ATTCAAGTTG CCCCTTTGAG TTTACCGTCT 
TTCGAAGAAC CTGACTTTTT GTCCACTCCT AGAGTAAAAC CTGATTCTCA GGGACCTCGG 
AGCAAAGTTA GTACACGACG TACTATTCTC GAACGAGACA ACAATTTACC AGTTAAGCGG 
GAGGAAAATA CTATCATTAA TTCAGAAACT GAAAGCACAA CACATAGCGC ACCATTTTTA 
AAAGAGGATC CGAAACCCTC GCCTCCTTCG AGTAAATTAT ACAACCCAAA AGTTCGTTTA 
AACAAAGCAG AAGCGCGAAA GTATACTGAT TCTTCTGCCC AAAGGACTAC TTCAGCAGGC 
TCGGTACTTG AAGACACTTC TATGCATAAA AAGAAAAAGA GTATATTTTC ATTTCTGAAG 
AAGAAAGAAC CGAAACCAGT TATTGGTAAT AATAGTGTAA CGAATGAAAA AAATAAGATG 
TCTTCATCGA GCACTTTCTC AATGAATATT CAAACCTCTT TAAAAACGCC TGAAAAACTA 
AAGAAGAAGT CTCACAGTAG CAGTAGCATT TTCAATTCCT TCCTAAAGGG AAAAATTGAA 
ACATCAGACT CACCTCGAAA GGAACCAATG AGGCAAAAAA AAAGAACCCC AAAATCCAAG 
GACAAAAAGC AAGATACTGA ACAAATAATT GACGCCGCAT CAGTGTTATC CACTGAATCA 
CCTCTACTAC GAAAAAACCA CGACGATACT CCCGTGAAAA TAGATCACGT AACGAGATCA 
ATTGATCAGA GAAAACCTAC TCCTTTAAAC ATGGATCTCA TATTGGGAGG TGATAAACAG 
ATAAATACCC CGCTACAAGA ACACGTAAGA GAAGATGATG ATGCCAAAAA TGATCTTCAA 
TTACCAACCA AGGATAATTT TTTATCACTG GACTACGAAG CACCCTCACC AGCATTTTCC 
AAACACGATA CAGGTGAGGT TCTTTTCCCA AAATTTTTGG ATAATCATGA AGTCGACTCG 
ATCGTTTCTT TAGAAAGAAC AAGATCTACA AAGTCTAACA AGAGAAGTTC AATGAACTCG 
CAAAGAAGGT CATTGACAGA TACGCTATCG ATCAAAGCGC AAAGTGAGGG AATGTTCATC 
ACTGAGGCAT CTTCTGTTGT ATTATCTACT CCAGACTTGA CGAAGTCTCC TGCTAGCAGC 
ATTTTAAAGA ATGGAAGGTT CGAATACTCA GATAATTTCT CCAGAGAACA CTCTTACGAA 
GGTACCACGA ATGAGGACTT TTTAGATATC AAGGACGATA GTGGTCCACT AAAAAAAGAT 
GACATATTTT TAGAGTCTAT TGAACAAAAA TTTGACCAGC TAGTGATGGC CTCTGATGAA 
GAAAAAACTG AAGTTGAAAG GGATGTACCA AAACCAAGAG AAGAACCTCT GAAGAAGGAT 
TCTGAAAGGC AAAGCGTTTT TGCAGATGAT GATAACGAGT TAATTTCTGA TATAATGGAG 
TTTGCAAGTT TTATTAACTT CGGTGATGAT GATCTCAATC TAGACCTAGA CTTGGGAGAT 
ACAACGGCCT CATATGCAAC AGAAACCCCT GAACCTGTTG GAAATGATGA AGTAAATCGC 
TCTGGTACTT TTGATACACG AAATAACAAA GAGGATAGTT ACAAAGAAAG AGAAACTCAA 
TCTTATTCCG CTGCTGGAGC TACTACATAC GGAGATGAGC GTCAAGGTCA ATTACATACA 
TTTGAACAAG ACGGAAGTGA AATAAATGAC AACGAATTTG AGAATGAAGA TTTCAACAAA 
CACATAGAAC AGCCCATAGA AGTTACTCCA AGAAATAATG CATATTTACC AGAATTCGAA 
CCCAATAGAC CTGTTTCAAT GTCCTTTAAA GGACTAAAAG CCCCAAGAAT GAATACTTCT 
TTTATTGACT CCATGACGCC TGATTCTCCC GTAAAATCAG ACTTAACAAG TCTAGGTGAG 
GTGTATGTAA ACAGTAATAA TGACCAGGGT GTCCGGTTTT CGTCCCAGAT AATACTTTAC 
GACACTTATG GAGAATTCGA ATATGATAGA CATCCAGAAA TTTCCACTTG TAACCAGCTT 
ACTCCACAAC TGGCTCAAAT GATCAAATTG GAATTAAATG AGTTAAAGAG CGCAATGGAA 
GTACACGATG ATTCGCGATG TTACACACAT TTTTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_014166.1
CDS	1..2679
Translation	MSDSISDSKSSELLNSTFYSSTSINTLDHARTFRNSLILKEISDQSLNSSIKPCESVLDRDVESSVLQRSFGESNARDSEVQTVNMTTSPSLSALADILNERSKYADQKTRKAQNIESSIIEEEEEAEEQNNSINYHEDITGSRLSVREEANENLAMTSPNLIDIDGSNSIQVAPLSLPSFEEPDFLSTPRVKPDSQGPRSKVSTRRTILERDNNLPVKREENTIINSETESTTHSAPFLKEDPKPSPPSSKLYNPKVRLNKAEARKYTDSSAQRTTSAGSVLEDTSMHKKKKSIFSFLKKKEPKPVIGNNSVTNEKNKMSSSSTFSMNIQTSLKTPEKLKKKSHSSSSIFNSFLKGKIETSDSPRKEPMRQKKRTPKSKDKKQDTEQIIDAASVLSTESPLLRKNHDDTPVKIDHVTRSIDQRKPTPLNMDLILGGDKQINTPLQEHVREDDDAKNDLQLPTKDNFLSLDYEAPSPAFSKHDTGEVLFPKFLDNHEVDSIVSLERTRSTKSNKRSSMNSQRRSLTDTLSIKAQSEGMFITEASSVVLSTPDLTKSPASSILKNGRFEYSDNFSREHSYEGTTNEDFLDIKDDSGPLKKDDIFLESIEQKFDQLVMASDEEKTEVERDVPKPREEPLKKDSERQSVFADDDNELISDIMEFASFINFGDDDLNLDLDLGDTTASYATETPEPVGNDEVNRSGTFDTRNNKEDSYKERETQSYSAAGATTYGDERQGQLHTFEQDGSEINDNEFENEDFNKHIEQPIEVTPRNNAYLPEFEPNRPVSMSFKGLKAPRMNTSFIDSMTPDSPVKSDLTSLGEVYVNSNNDQGVRFSSQIILYDTYGEFEYDRHPEISTCNQLTPQLAQMIKLELNELKSAMEVHDDSRCYTHFY

Target ORF information:

RefSeq Version	NM_001183071.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Bni4p (BNI4), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001183071.1

ORF Insert Sequence:

ATGTCGGATA GTATTTCAGA TTCAAAGTCC TCAGAACTTT TAAATTCTAC TTTTTATTCG 
TCGACGTCGA TAAATACACT TGATCATGCT AGAACCTTTC GAAATTCTCT GATATTGAAG 
GAGATATCAG ATCAAAGTCT AAATTCATCA ATAAAGCCAT GCGAATCCGT CCTTGATAGG 
GATGTTGAAA GCTCAGTTCT GCAGCGGTCG TTTGGAGAAA GTAATGCCCG AGACTCAGAA 
GTACAAACGG TAAATATGAC AACTTCCCCC TCATTAAGTG CATTGGCTGA TATTCTGAAC 
GAAAGATCCA AATATGCTGA CCAAAAAACT AGAAAGGCAC AAAATATTGA GTCTTCCATA 
ATAGAAGAAG AAGAAGAGGC CGAAGAGCAA AACAACTCGA TCAATTATCA CGAGGATATA 
ACTGGATCCA GGCTGTCTGT ACGAGAGGAA GCAAATGAAA ACTTGGCGAT GACCTCTCCA 
AACCTTATTG ACATTGACGG ATCCAATAGT ATTCAAGTTG CCCCTTTGAG TTTACCGTCT 
TTCGAAGAAC CTGACTTTTT GTCCACTCCT AGAGTAAAAC CTGATTCTCA GGGACCTCGG 
AGCAAAGTTA GTACACGACG TACTATTCTC GAACGAGACA ACAATTTACC AGTTAAGCGG 
GAGGAAAATA CTATCATTAA TTCAGAAACT GAAAGCACAA CACATAGCGC ACCATTTTTA 
AAAGAGGATC CGAAACCCTC GCCTCCTTCG AGTAAATTAT ACAACCCAAA AGTTCGTTTA 
AACAAAGCAG AAGCGCGAAA GTATACTGAT TCTTCTGCCC AAAGGACTAC TTCAGCAGGC 
TCGGTACTTG AAGACACTTC TATGCATAAA AAGAAAAAGA GTATATTTTC ATTTCTGAAG 
AAGAAAGAAC CGAAACCAGT TATTGGTAAT AATAGTGTAA CGAATGAAAA AAATAAGATG 
TCTTCATCGA GCACTTTCTC AATGAATATT CAAACCTCTT TAAAAACGCC TGAAAAACTA 
AAGAAGAAGT CTCACAGTAG CAGTAGCATT TTCAATTCCT TCCTAAAGGG AAAAATTGAA 
ACATCAGACT CACCTCGAAA GGAACCAATG AGGCAAAAAA AAAGAACCCC AAAATCCAAG 
GACAAAAAGC AAGATACTGA ACAAATAATT GACGCCGCAT CAGTGTTATC CACTGAATCA 
CCTCTACTAC GAAAAAACCA CGACGATACT CCCGTGAAAA TAGATCACGT AACGAGATCA 
ATTGATCAGA GAAAACCTAC TCCTTTAAAC ATGGATCTCA TATTGGGAGG TGATAAACAG 
ATAAATACCC CGCTACAAGA ACACGTAAGA GAAGATGATG ATGCCAAAAA TGATCTTCAA 
TTACCAACCA AGGATAATTT TTTATCACTG GACTACGAAG CACCCTCACC AGCATTTTCC 
AAACACGATA CAGGTGAGGT TCTTTTCCCA AAATTTTTGG ATAATCATGA AGTCGACTCG 
ATCGTTTCTT TAGAAAGAAC AAGATCTACA AAGTCTAACA AGAGAAGTTC AATGAACTCG 
CAAAGAAGGT CATTGACAGA TACGCTATCG ATCAAAGCGC AAAGTGAGGG AATGTTCATC 
ACTGAGGCAT CTTCTGTTGT ATTATCTACT CCAGACTTGA CGAAGTCTCC TGCTAGCAGC 
ATTTTAAAGA ATGGAAGGTT CGAATACTCA GATAATTTCT CCAGAGAACA CTCTTACGAA 
GGTACCACGA ATGAGGACTT TTTAGATATC AAGGACGATA GTGGTCCACT AAAAAAAGAT 
GACATATTTT TAGAGTCTAT TGAACAAAAA TTTGACCAGC TAGTGATGGC CTCTGATGAA 
GAAAAAACTG AAGTTGAAAG GGATGTACCA AAACCAAGAG AAGAACCTCT GAAGAAGGAT 
TCTGAAAGGC AAAGCGTTTT TGCAGATGAT GATAACGAGT TAATTTCTGA TATAATGGAG 
TTTGCAAGTT TTATTAACTT CGGTGATGAT GATCTCAATC TAGACCTAGA CTTGGGAGAT 
ACAACGGCCT CATATGCAAC AGAAACCCCT GAACCTGTTG GAAATGATGA AGTAAATCGC 
TCTGGTACTT TTGATACACG AAATAACAAA GAGGATAGTT ACAAAGAAAG AGAAACTCAA 
TCTTATTCCG CTGCTGGAGC TACTACATAC GGAGATGAGC GTCAAGGTCA ATTACATACA 
TTTGAACAAG ACGGAAGTGA AATAAATGAC AACGAATTTG AGAATGAAGA TTTCAACAAA 
CACATAGAAC AGCCCATAGA AGTTACTCCA AGAAATAATG CATATTTACC AGAATTCGAA 
CCCAATAGAC CTGTTTCAAT GTCCTTTAAA GGACTAAAAG CCCCAAGAAT GAATACTTCT 
TTTATTGACT CCATGACGCC TGATTCTCCC GTAAAATCAG ACTTAACAAG TCTAGGTGAG 
GTGTATGTAA ACAGTAATAA TGACCAGGGT GTCCGGTTTT CGTCCCAGAT AATACTTTAC 
GACACTTATG GAGAATTCGA ATATGATAGA CATCCAGAAA TTTCCACTTG TAACCAGCTT 
ACTCCACAAC TGGCTCAAAT GATCAAATTG GAATTAAATG AGTTAAAGAG CGCAATGGAA 
GTACACGATG ATTCGCGATG TTACACACAT TTTTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Nature387(6632 Suppl)93-8(1997 May)
Philippsen P,Kleine K,P?hlmann R,D?sterh?ft A,Hamberg K,Hegemann JH,Obermaier B,Urrestarazu LA,Aert R,Albermann K,Altmann R,Andr? B,Baladron V,Ballesta JP,B?cam AM,Beinhauer J,Boskovic J,Buitrago MJ,Bussereau F,Coster F,Crouzet M,D'Angelo M,Dal Pero F,De Antoni A,Del Rey F,Doignon F,Domdey H,Dubois E,Fiedler T,Fleig U,Floeth M,Fritz C,Gaillardin C,Garcia-Cantalejo JM,Glansdorff NN,Goffeau A,Gueldener U,Herbert C,Heumann K,Heuss-Neitzel D,Hilbert H,Hinni K,Iraqui Houssaini I,Jacquet M,Jimenez A,Jonniaux JL,Karpfinger L,Lanfranchi G,Lepingle A,Levesque H,Lyck R,Maftahi M,Mallet L,Maurer KC,Messenguy F,Mewes HW,M?sti D,Nasr F,Nicaud JM,Niedenthal RK,Pandolfo D,Pi?rard A,Piravandi E,Planta RJ,Pohl TM,Purnelle B,Rebischung C,Remacha M,Revuelta JL,Rinke M,Saiz JE,Sartorello F,Scherens B,Sen-Gupta M,Soler-Mira A,Urbanus JH,Valle G,Van Dyck L,Verhasselt P,Vierendeels F,Vissers S,Voet M,Volckaert G,Wach A,Wambutt R,Wedler H,Zollner A,Hani J

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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BNI4 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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