BNI1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	BNI1
Entrez Gene ID	855450
Full Name	formin BNI1
Synonyms	PPF3,SHE5
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001183109.2	NP_014128.2	formin BNI1

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	BNI1	NP_014128.2
Kluyveromyces lactis	KLLA0C02321g	XP_452300.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Philippsen P, Kleine K, P?hlmann R, D?sterh?ft A, Hamberg K, Hegemann JH, Obermaier B, Urrestarazu LA, Aert R, Albermann K, Altmann R, Andr? B, Baladron V, Ballesta JP, B?cam AM, Beinhauer J, Boskovic J, Buitrago MJ, Bussereau F, Coster F, Crouzet M, D'Angelo M, Dal Pero F, De Antoni A, Del Rey F, Doignon F, Domdey H, Dubois E, Fiedler T, Fleig U, Floeth M, Fritz C, Gaillardin C, Garcia-Cantalejo JM, Glansdorff NN, Goffeau A, Gueldener U, Herbert C, Heumann K, Heuss-Neitzel D, Hilbert H, Hinni K, Iraqui Houssaini I, Jacquet M, Jimenez A, Jonniaux JL, Karpfinger L, Lanfranchi G, Lepingle A, Levesque H, Lyck R, Maftahi M, Mallet L, Maurer KC, Messenguy F, Mewes HW, M?sti D, Nasr F, Nicaud JM, Niedenthal RK, Pandolfo D, Pi?rard A, Piravandi E, Planta RJ, Pohl TM, Purnelle B, Rebischung C, Remacha M, Revuelta JL, Rinke M, Saiz JE, Sartorello F, Scherens B, Sen-Gupta M, Soler-Mira A, Urbanus JH, Valle G, Van Dyck L, Verhasselt P, Vierendeels F, Vissers S, Voet M, Volckaert G, Wach A, Wambutt R, Wedler H, Zollner A, Hani J
Nature387(6632 Suppl)93-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Expression of a hyperactive variant of Bni1 (Bni1-V360D) rescues these defects and partially restores proper spindle positioning in the mutant, suggesting that the failure of dma1 dma2 mutant cells to position the spindle is partly due to faulty formin activity.
Title: Control of Formin Distribution and Actin Cable Assembly by the E3 Ubiquitin Ligases Dma1 and Dma2.

Myo1 plays a major role in the septin-dependent Mlc1 localization to the division site before cytokinesis, whereas the formin Bni1 plays a major role in the actin filament-dependent Mlc1 localization during cytokinesis.
Title: Myosin?II heavy chain and formin mediate the targeting of myosin essential light chain to the division site before and during cytokinesis.

Data indicate that small forces on the filaments dramatically slowed formin Bni1p-mediated polymerization in the absence of profilin, but resulted in faster polymerization in the presence of profilin.
Title: Tension modulates actin filament polymerization mediated by formin and profilin.

F-actin stabilization is regulated by yeast formins Bni1p and Bnr1p
Title: Importance of a Lys113-Glu195 intermonomer ionic bond in F-actin stabilization and regulation by yeast formins Bni1p and Bnr1p.

A novel regulatory input to Bni1p acting through the Cdc42p effector, Gic2p.
Title: Cdc42p regulation of the yeast formin Bni1p mediated by the effector Gic2p.

The following BNI1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BNI1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04379	NM_001183109.2 Latest version!	Saccharomyces cerevisiae S288C formin BNI1 (BNI1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BNI1 ( NM_001183109.2 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_014128.2 Saccharomyces cerevisiae S288C formin BNI1 (BNI1), partial mRNA.

Price & Availability↑

CloneID	OSi04379
Clone ID Related Accession (Same CDS sequence)	NM_001183109.2
Accession Version	NM_001183109.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5862bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	formin BNI1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001146). On Apr 26, 2011 this sequence version replaced NM_001183109.1. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTTGAAGA ATTCAGGCTC CAAACATTCG AACTCAAAGG AAAGTCATTC GAATTCGAGT 
TCTGGTATAT TCCAAAATTT GAAGCGTTTG GCTAACTCAA ATGCAACGAA TAGCAATACG 
GGTTCTCCGA CCTATGCATC CCAACAACAG CACTCTCCAG TGGGAAACGA AGTGTCCACT 
TCACCTGCAT CTTCGTCATC TTTTAGAAAA CTGAATGCAC CTTCTAGGTC TACATCTACT 
GAGGCGAGGC CGTTGAATAA AAAATCGACA TTAAACACAC AAAACTTGTC TCAATATATG 
AATGGTAAAT TAAGTGGAGA TGTTCCCGTA TCCTCACAGC ACGCAAGGTC GCATTCAATG 
CAATCGAAAT ATTCGTATTC CAAGAGGAAT TCTTCACAGG CGTCCAATAA GCTAACAAGG 
CAGCATACCG GGCAAAGTCA CTCCGCATCA AGTCTTCTCT CTCAAGGTTC GCTAACTAAC 
TTGAGCAAAT TCACTACACC CGATGGTAAA ATTTATTTAG AAATGCCGTC GGACCCATAC 
GAGGTGGAAG TTTTGTTTGA AGATATTATG TATAAAAGAA ATATTTTTCA GTCTTTATCA 
GAGGACAAAC AAGAAGCTCT GATGGGCTAC AGCATTGAGA AGAAATGGCT GATTGTTAAG 
CAGGATTTAC AAAATGAGCT AAAAAAAATG CGAGCAAACA CTACATCTTC TTCCACTGCC 
TCCAGAACTT CGATGGCGTC GGACCATCAT CCTATCCTTA CGGCTAATTC ATCTCTTTCG 
TCTCCCAAAT CTGTTTTGAT GACAAGCGCC TCTTCTCCCA CCTCAACTGT ATACAGCAAC 
AGTTTAAATC ATTCTACTAC TCTTTCATCG GTAGGTACGT CAACCTCGAA GGGGAAAAAA 
TTAGTGAGCG GGAGTTTGAA AAAGCAGCCA TCTCTAAACA ATATTTATAG AGGTGGGGCT 
GAGAACAATA CGAGTGCATC TACTCTTCCA GGAGACAGAA CAAACAGACC ACCGATACAT 
TATGTGCAAC GAATCCTGGC AGACAAACTT ACTAGCGATG AGATGAAAGA TTTATGGGTA 
ACTTTAAGAA CTGAACAGTT AGACTGGGTA GACGCTTTTA TAGACCATCA GGGTCACATT 
GCTATGGCTA ATGTACTAAT GAACTCTATT TATAAGACTG CACCCCGAGA AAACTTGACT 
AAAGAATTAT TAGAGAAAGA AAATTCATTT TTTAAATGCT TCAGAGTCTT ATCAATGCTC 
TCACAAGGTT TGTATGAGTT CAGTACACAT AGGTTAATGA CTGATACTGT TGCAGAAGGT 
TTATTCTCTA CGAAACTGGC CACAAGGAAA ATGGCCACTG AAATTTTTGT TTGTATGCTA 
GAAAAAAAAA ATAAAAGCAG ATTCGAAGCA GTTCTTACCT CACTAGATAA AAAGTTTAGG 
ATAGGGCAAA ATCTTCATAT GATTCAAAAC TTCAAAAAAA TGCCCCAGTA TTTCTCTCAT 
TTAACCTTGG AAAGTCATTT GAAAATTATT CAAGCCTGGT TATTTGCCGT TGAACAAACT 
TTAGATGGAA GAGGAAAAAT GGGTTCCTTA GTAGGCGCGT CGGATGAATT TAAAAATGGT 
GGGGGTGAAA ATGCGATTCT AGAATACTGC CAATGGACAA TGGTTTTTAT CAATCATTTA 
TGCTCTTGCT CTGATAACAT AAATCAAAGA ATGCTATTAA GGACGAAACT AGAAAATTGT 
GGAATTTTAC GAATTATGAA CAAAATAAAA CTGCTAGATT ACGACAAGGT AATTGACCAA 
ATTGAGTTAT ATGACAATAA TAAACTTGAC GATTTTAACG TTAAATTAGA GGCCAACAAT 
AAGGCTTTTA ATGTAGATTT ACACGATCCA CTATCATTAT TAAAAAACCT TTGGGATATA 
TGTAAAGGTA CCGAGAATGA AAAGCTTTTA GTATCTTTAG TTCAACATCT TTTCCTTTCT 
AGTTCAAAGT TAATAGAAGA AAACCAAAAT TCCTCTAAAC TTACTAAGCA ATTGAAACTT 
ATGGATTCTT TGGTGACTAA TGTTAGTGTT GCCTCAACTT CTGATGAAGA AACTAATATG 
AATATGGCTA TCCAGCGGCT TTACGATGCC ATGCAGACTG ATGAAGTTGC ACGTCGAGCT 
ATATTAGAAA GTAGAGCTCT AACGAAGAAA TTGGAAGAAA TTCAGGCAGA AAGGGATTCT 
TTAAGTGAAA AGTTAAGCAA GGCGGAGCAC GGACTCGTTG GACAGTTAGA AGATGAGTTG 
CATGAGAGAG ATCGTATTTT AGCTAAGAAT CAGAGAGTTA TGCAGCAGCT GGAAGCTGAG 
TTAGAAGAGT TGAAAAAGAA GCATCTTTTG GAAAAGCATC AGCAAGAGGT AGAATTGAGA 
AAAATGTTGA CTATATTGAA TTCGAGGCCT GAAGAAAGCT TCAATAAGAA TGAAGGCACC 
AGAGGTATGA ACTCGAGCCT AAATTCTTCA GAGAAAGCGA ATATCCAAAA GGTGTTACAG 
GACGGATTAT CAAGAGCAAA AAAGGATTAC AAGGATGATT CAAAAAAATT CGGCATGACA 
CTTCAACCTA ATAAGAGATT AAAAATGTTA AGAATGCAAA TGGAAAATAT TGAAAATGAA 
GCCAGGCAAC TAGAGATGAC AAATTTTGCT GAATTTGAGA AAGATCGTCT TGAACCTCCA 
ATACATATTA AGAAGCCCAA AGTGAAGAAG ATGAAAAATA AGGATAGGAA ACCTTTAGTC 
AAGCCTCAAG AGGCGGACGT AAATAAACTA AATGACCTAA GGCGGGCTTT GGCTGAAATC 
CAAATGGAAA GTAATGATAT TTCCAAATTT AACGTCGAAG AACGTGTTAA TGAGCTATTT 
AATGAAAAGA AATCTCTGGC TTTAAAGAGA CTGAAAGAAC TAGAAACAAA ATACAAAGGA 
TTTGGTATTG ACTTTAATGT CGACGAGATT ATGGACAGTC CAAAGAAAAA TACTGGGGAC 
GTGGAAACGG AAGAAGACGC TAACTATGCC AGTCTTGACC CTAAGACATA TCAGAAAAAG 
TTAGACGAGA TTAATAGAAT AACGGACCAG TTGTTAGATA TTCAGACTCA AACCGAACAC 
GAAATACAAG TAGAAGAAGA TGGGGAAAGC GATCTTTCCT CTTCAAGTTC TGATGATGAA 
AGTGAGGAAA TATACCAAGA CGCATCTCCA ACTCAAGAAC TAAGGAGCGA ACACTCAGAG 
CTATCTTCAG GATCAGGTCC TGGGTCTTTT CTCGATGCCT TATCTCAAAA ATATGGTACT 
GGTCAAAATG TTACTGCATC GGCTGCTTTT GGAGAGAACA ATAACGGTTC GGGTATTGGA 
CCTCTACATA GTAAAGTTGA AAAGACCTTT ATGAACAGAC TCAGAAAATC CACCGTGTCT 
TCTGCTCCAT ATTTAGAGGA GTTAACACAG AAGGTAAATA AAGTTGAACC ATATGAACAA 
AATGAAGATG AAGGTCTAGA TAAAAAATCT TTACCGGAAA ACAGCACGGC AAGTGCAGCT 
TCCGCCTTTG ACAAAGCTGA GAAAGACATG AGACAACATG TGGAAAACGG AAAGCAAGGG 
CGTGTGGTAA ATCATGAAGA AGATAAAACA GCGGATTTCA GCGCTGTGAG TAAACTGAAT 
AATACAGATG GCGCAGAAGA TCTCTCTACT CAATCATCTG TACTCTCCTC ACAGCCGCCA 
CCGCCTCCTC CTCCTCCTCC TCCAGTTCCC GCCAAATTGT TCGGTGAATC GTTGGAGAAA 
GAGAAAAAGT CGGAAGACGA CACTGTCAAG CAAGAAACTA CTGGGGATTC TCCTGCTCCT 
CCCCCTCCAC CACCACCACC TCCTCCTCCA CCCATGGCAT TGTTCGGCAA ACCCAAGGGA 
GAAACACCAC CACCGCCGCC GCTACCATCT GTATTATCTT CATCTACTGA TGGCGTCATT 
CCGCCAGCTC CACCTATGAT GCCGGCATCG CAAATCAAAT CAGCTGTAAC ATCACCATTA 
CTTCCTCAAT CGCCCTCATT ATTTGAAAAG TATCCTCGTC CACACAAGAA ATTAAAGCAA 
CTGCATTGGG AAAAATTAGA TTGTACTGAT AATTCTATTT GGGGTACAGG TAAAGCTGAG 
AAGTTCGCAG ATGACTTATA CGAAAAGGGT GTTCTTGCTG ATTTAGAAAA GGCCTTTGCC 
GCTAGGGAAA TTAAATCATT AGCAAGCAAG CGCAAGGAAG ATCTGCAGAA AATTACATTT 
TTATCCAGGG ATATTTCTCA ACAGTTTGGT ATCAATTTGC ATATGTATTC ATCACTTTCG 
GTTGCCGATC TGGTGAAAAA GATTCTAAAC TGCGATAGGG ATTTTTTGCA AACACCGAGT 
GTTGTTGAGT TTTTGTCAAA ATCTGAAATA ATTGAAGTTT CTGTTAATTT AGCTAGGAAT 
TACGCCCCGT ATTCGACAGA CTGGGAGGGC GTGAGGAATC TCGAAGATGC CAAGCCCCCT 
GAAAAAGATC CAAACGATTT ACAGAGGGCT GACCAAATTT ATTTGCAATT GATGGTTAAT 
CTAGAATCAT ACTGGGGTTC ACGTATGAGA GCTCTCACAG TGGTTACTTC GTATGAAAGA 
GAATATAATG AGTTATTGGC AAAGTTAAGA AAAGTCGACA AAGCTGTTAG CGCGCTTCAA 
GAATCAGATA ACTTGCGCAA TGTCTTTAAT GTTATCTTAG CAGTTGGTAA TTTCATGAAC 
GACACTTCTA AGCAGGCACA AGGTTTCAAA CTTTCAACCT TGCAGAGGTT GACGTTTATC 
AAGGATACTA CCAATAGCAT GACCTTTCTA AATTACGTCG AAAAAATTGT CAGGTTAAAT 
TATCCAAGCT TCAATGACTT TCTAAGTGAA TTAGAACCTG TATTGGATGT CGTGAAAGTT 
TCCATTGAGC AGTTAGTTAA CGACTGTAAG GACTTTAGCC AATCGATTGT AAACGTAGAG 
CGTTCAGTTG AAATTGGTAA TTTAAGCGAT TCCTCAAAGT TTCATCCATT AGATAAAGTT 
TTGATAAAAA CATTGCCCGT GTTACCGGAA GCCAGAAAAA AGGGGGACTT GTTAGAAGAC 
GAAGTTAAGC TGACTATCAT GGAATTTGAA AGTCTGATGC ATACGTACGG TGAAGATTCT 
GGTGATAAGT TTGCAAAAAT CTCATTCTTT AAGAAATTTG CAGATTTTAT AAATGAATAC 
AAAAAAGCTC AAGCGCAAAA TCTAGCCGCG GAAGAAGAAG AAAGACTTTA TATAAAGCAT 
AAGAAAATAG TGGAAGAACA GCAAAAAAGG GCACAAGAAA AGGAAAAACA AAAAGAGAAC 
AGCAATTCGC CTTCATCTGA AGGAAATGAA GAGGATGAAG CAGAAGATAG GCGCGCTGTT 
ATGGATAAAC TATTAGAACA GTTGAAAAAT GCAGGTCCTG CTAAAAGTGA CCCATCTTCC 
GCCAGAAAGA GAGCCCTAGT GAGGAAAAAA TATCTTTCTG AAAAAGACAA CGCTCCACAA 
TTACTCAACG ATTTGGATAC TGAAGAAGGG TCTATTCTTT ATTCTCCAGA AGCTATGGAC 
CCTACTGCCG ATACAGTGAT TCACGCTGAA TCACCCACTC CGCTTGCCAC AAGGGGTGTT 
ATGAATACCT CTGAAGATTT ACCATCGCCA TCCAAGACAT CTGCGCTGGA AGATCAAGAG 
GAAATTAGTG ACAGAGCTAG AATGTTATTA AAGGAGCTAA GAGGCTCTGA CACACCAGTA 
AAGCAAAATT CCATACTCGA TGAACATCTA GAAAAATTGA GGGCCCGCAA GGAAAGATCC 
ATAGGTGAGG CTAGCACAGG TAACAGGCTA AGTTTCAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_014128.2
CDS	1..5862
Translation	MLKNSGSKHSNSKESHSNSSSGIFQNLKRLANSNATNSNTGSPTYASQQQHSPVGNEVSTSPASSSSFRKLNAPSRSTSTEARPLNKKSTLNTQNLSQYMNGKLSGDVPVSSQHARSHSMQSKYSYSKRNSSQASNKLTRQHTGQSHSASSLLSQGSLTNLSKFTTPDGKIYLEMPSDPYEVEVLFEDIMYKRNIFQSLSEDKQEALMGYSIEKKWLIVKQDLQNELKKMRANTTSSSTASRTSMASDHHPILTANSSLSSPKSVLMTSASSPTSTVYSNSLNHSTTLSSVGTSTSKGKKLVSGSLKKQPSLNNIYRGGAENNTSASTLPGDRTNRPPIHYVQRILADKLTSDEMKDLWVTLRTEQLDWVDAFIDHQGHIAMANVLMNSIYKTAPRENLTKELLEKENSFFKCFRVLSMLSQGLYEFSTHRLMTDTVAEGLFSTKLATRKMATEIFVCMLEKKNKSRFEAVLTSLDKKFRIGQNLHMIQNFKKMPQYFSHLTLESHLKIIQAWLFAVEQTLDGRGKMGSLVGASDEFKNGGGENAILEYCQWTMVFINHLCSCSDNINQRMLLRTKLENCGILRIMNKIKLLDYDKVIDQIELYDNNKLDDFNVKLEANNKAFNVDLHDPLSLLKNLWDICKGTENEKLLVSLVQHLFLSSSKLIEENQNSSKLTKQLKLMDSLVTNVSVASTSDEETNMNMAIQRLYDAMQTDEVARRAILESRALTKKLEEIQAERDSLSEKLSKAEHGLVGQLEDELHERDRILAKNQRVMQQLEAELEELKKKHLLEKHQQEVELRKMLTILNSRPEESFNKNEGTRGMNSSLNSSEKANIQKVLQDGLSRAKKDYKDDSKKFGMTLQPNKRLKMLRMQMENIENEARQLEMTNFAEFEKDRLEPPIHIKKPKVKKMKNKDRKPLVKPQEADVNKLNDLRRALAEIQMESNDISKFNVEERVNELFNEKKSLALKRLKELETKYKGFGIDFNVDEIMDSPKKNTGDVETEEDANYASLDPKTYQKKLDEINRITDQLLDIQTQTEHEIQVEEDGESDLSSSSSDDESEEIYQDASPTQELRSEHSELSSGSGPGSFLDALSQKYGTGQNVTASAAFGENNNGSGIGPLHSKVEKTFMNRLRKSTVSSAPYLEELTQKVNKVEPYEQNEDEGLDKKSLPENSTASAASAFDKAEKDMRQHVENGKQGRVVNHEEDKTADFSAVSKLNNTDGAEDLSTQSSVLSSQPPPPPPPPPPVPAKLFGESLEKEKKSEDDTVKQETTGDSPAPPPPPPPPPPPPMALFGKPKGETPPPPPLPSVLSSSTDGVIPPAPPMMPASQIKSAVTSPLLPQSPSLFEKYPRPHKKLKQLHWEKLDCTDNSIWGTGKAEKFADDLYEKGVLADLEKAFAAREIKSLASKRKEDLQKITFLSRDISQQFGINLHMYSSLSVADLVKKILNCDRDFLQTPSVVEFLSKSEIIEVSVNLARNYAPYSTDWEGVRNLEDAKPPEKDPNDLQRADQIYLQLMVNLESYWGSRMRALTVVTSYEREYNELLAKLRKVDKAVSALQESDNLRNVFNVILAVGNFMNDTSKQAQGFKLSTLQRLTFIKDTTNSMTFLNYVEKIVRLNYPSFNDFLSELEPVLDVVKVSIEQLVNDCKDFSQSIVNVERSVEIGNLSDSSKFHPLDKVLIKTLPVLPEARKKGDLLEDEVKLTIMEFESLMHTYGEDSGDKFAKISFFKKFADFINEYKKAQAQNLAAEEEERLYIKHKKIVEEQQKRAQEKEKQKENSNSPSSEGNEEDEAEDRRAVMDKLLEQLKNAGPAKSDPSSARKRALVRKKYLSEKDNAPQLLNDLDTEEGSILYSPEAMDPTADTVIHAESPTPLATRGVMNTSEDLPSPSKTSALEDQEEISDRARMLLKELRGSDTPVKQNSILDEHLEKLRARKERSIGEASTGNRLSFK

Target ORF information:

RefSeq Version	NM_001183109.2
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C formin BNI1 (BNI1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001183109.2

ORF Insert Sequence:

ATGTTGAAGA ATTCAGGCTC CAAACATTCG AACTCAAAGG AAAGTCATTC GAATTCGAGT 
TCTGGTATAT TCCAAAATTT GAAGCGTTTG GCTAACTCAA ATGCAACGAA TAGCAATACG 
GGTTCTCCGA CCTATGCATC CCAACAACAG CACTCTCCAG TGGGAAACGA AGTGTCCACT 
TCACCTGCAT CTTCGTCATC TTTTAGAAAA CTGAATGCAC CTTCTAGGTC TACATCTACT 
GAGGCGAGGC CGTTGAATAA AAAATCGACA TTAAACACAC AAAACTTGTC TCAATATATG 
AATGGTAAAT TAAGTGGAGA TGTTCCCGTA TCCTCACAGC ACGCAAGGTC GCATTCAATG 
CAATCGAAAT ATTCGTATTC CAAGAGGAAT TCTTCACAGG CGTCCAATAA GCTAACAAGG 
CAGCATACCG GGCAAAGTCA CTCCGCATCA AGTCTTCTCT CTCAAGGTTC GCTAACTAAC 
TTGAGCAAAT TCACTACACC CGATGGTAAA ATTTATTTAG AAATGCCGTC GGACCCATAC 
GAGGTGGAAG TTTTGTTTGA AGATATTATG TATAAAAGAA ATATTTTTCA GTCTTTATCA 
GAGGACAAAC AAGAAGCTCT GATGGGCTAC AGCATTGAGA AGAAATGGCT GATTGTTAAG 
CAGGATTTAC AAAATGAGCT AAAAAAAATG CGAGCAAACA CTACATCTTC TTCCACTGCC 
TCCAGAACTT CGATGGCGTC GGACCATCAT CCTATCCTTA CGGCTAATTC ATCTCTTTCG 
TCTCCCAAAT CTGTTTTGAT GACAAGCGCC TCTTCTCCCA CCTCAACTGT ATACAGCAAC 
AGTTTAAATC ATTCTACTAC TCTTTCATCG GTAGGTACGT CAACCTCGAA GGGGAAAAAA 
TTAGTGAGCG GGAGTTTGAA AAAGCAGCCA TCTCTAAACA ATATTTATAG AGGTGGGGCT 
GAGAACAATA CGAGTGCATC TACTCTTCCA GGAGACAGAA CAAACAGACC ACCGATACAT 
TATGTGCAAC GAATCCTGGC AGACAAACTT ACTAGCGATG AGATGAAAGA TTTATGGGTA 
ACTTTAAGAA CTGAACAGTT AGACTGGGTA GACGCTTTTA TAGACCATCA GGGTCACATT 
GCTATGGCTA ATGTACTAAT GAACTCTATT TATAAGACTG CACCCCGAGA AAACTTGACT 
AAAGAATTAT TAGAGAAAGA AAATTCATTT TTTAAATGCT TCAGAGTCTT ATCAATGCTC 
TCACAAGGTT TGTATGAGTT CAGTACACAT AGGTTAATGA CTGATACTGT TGCAGAAGGT 
TTATTCTCTA CGAAACTGGC CACAAGGAAA ATGGCCACTG AAATTTTTGT TTGTATGCTA 
GAAAAAAAAA ATAAAAGCAG ATTCGAAGCA GTTCTTACCT CACTAGATAA AAAGTTTAGG 
ATAGGGCAAA ATCTTCATAT GATTCAAAAC TTCAAAAAAA TGCCCCAGTA TTTCTCTCAT 
TTAACCTTGG AAAGTCATTT GAAAATTATT CAAGCCTGGT TATTTGCCGT TGAACAAACT 
TTAGATGGAA GAGGAAAAAT GGGTTCCTTA GTAGGCGCGT CGGATGAATT TAAAAATGGT 
GGGGGTGAAA ATGCGATTCT AGAATACTGC CAATGGACAA TGGTTTTTAT CAATCATTTA 
TGCTCTTGCT CTGATAACAT AAATCAAAGA ATGCTATTAA GGACGAAACT AGAAAATTGT 
GGAATTTTAC GAATTATGAA CAAAATAAAA CTGCTAGATT ACGACAAGGT AATTGACCAA 
ATTGAGTTAT ATGACAATAA TAAACTTGAC GATTTTAACG TTAAATTAGA GGCCAACAAT 
AAGGCTTTTA ATGTAGATTT ACACGATCCA CTATCATTAT TAAAAAACCT TTGGGATATA 
TGTAAAGGTA CCGAGAATGA AAAGCTTTTA GTATCTTTAG TTCAACATCT TTTCCTTTCT 
AGTTCAAAGT TAATAGAAGA AAACCAAAAT TCCTCTAAAC TTACTAAGCA ATTGAAACTT 
ATGGATTCTT TGGTGACTAA TGTTAGTGTT GCCTCAACTT CTGATGAAGA AACTAATATG 
AATATGGCTA TCCAGCGGCT TTACGATGCC ATGCAGACTG ATGAAGTTGC ACGTCGAGCT 
ATATTAGAAA GTAGAGCTCT AACGAAGAAA TTGGAAGAAA TTCAGGCAGA AAGGGATTCT 
TTAAGTGAAA AGTTAAGCAA GGCGGAGCAC GGACTCGTTG GACAGTTAGA AGATGAGTTG 
CATGAGAGAG ATCGTATTTT AGCTAAGAAT CAGAGAGTTA TGCAGCAGCT GGAAGCTGAG 
TTAGAAGAGT TGAAAAAGAA GCATCTTTTG GAAAAGCATC AGCAAGAGGT AGAATTGAGA 
AAAATGTTGA CTATATTGAA TTCGAGGCCT GAAGAAAGCT TCAATAAGAA TGAAGGCACC 
AGAGGTATGA ACTCGAGCCT AAATTCTTCA GAGAAAGCGA ATATCCAAAA GGTGTTACAG 
GACGGATTAT CAAGAGCAAA AAAGGATTAC AAGGATGATT CAAAAAAATT CGGCATGACA 
CTTCAACCTA ATAAGAGATT AAAAATGTTA AGAATGCAAA TGGAAAATAT TGAAAATGAA 
GCCAGGCAAC TAGAGATGAC AAATTTTGCT GAATTTGAGA AAGATCGTCT TGAACCTCCA 
ATACATATTA AGAAGCCCAA AGTGAAGAAG ATGAAAAATA AGGATAGGAA ACCTTTAGTC 
AAGCCTCAAG AGGCGGACGT AAATAAACTA AATGACCTAA GGCGGGCTTT GGCTGAAATC 
CAAATGGAAA GTAATGATAT TTCCAAATTT AACGTCGAAG AACGTGTTAA TGAGCTATTT 
AATGAAAAGA AATCTCTGGC TTTAAAGAGA CTGAAAGAAC TAGAAACAAA ATACAAAGGA 
TTTGGTATTG ACTTTAATGT CGACGAGATT ATGGACAGTC CAAAGAAAAA TACTGGGGAC 
GTGGAAACGG AAGAAGACGC TAACTATGCC AGTCTTGACC CTAAGACATA TCAGAAAAAG 
TTAGACGAGA TTAATAGAAT AACGGACCAG TTGTTAGATA TTCAGACTCA AACCGAACAC 
GAAATACAAG TAGAAGAAGA TGGGGAAAGC GATCTTTCCT CTTCAAGTTC TGATGATGAA 
AGTGAGGAAA TATACCAAGA CGCATCTCCA ACTCAAGAAC TAAGGAGCGA ACACTCAGAG 
CTATCTTCAG GATCAGGTCC TGGGTCTTTT CTCGATGCCT TATCTCAAAA ATATGGTACT 
GGTCAAAATG TTACTGCATC GGCTGCTTTT GGAGAGAACA ATAACGGTTC GGGTATTGGA 
CCTCTACATA GTAAAGTTGA AAAGACCTTT ATGAACAGAC TCAGAAAATC CACCGTGTCT 
TCTGCTCCAT ATTTAGAGGA GTTAACACAG AAGGTAAATA AAGTTGAACC ATATGAACAA 
AATGAAGATG AAGGTCTAGA TAAAAAATCT TTACCGGAAA ACAGCACGGC AAGTGCAGCT 
TCCGCCTTTG ACAAAGCTGA GAAAGACATG AGACAACATG TGGAAAACGG AAAGCAAGGG 
CGTGTGGTAA ATCATGAAGA AGATAAAACA GCGGATTTCA GCGCTGTGAG TAAACTGAAT 
AATACAGATG GCGCAGAAGA TCTCTCTACT CAATCATCTG TACTCTCCTC ACAGCCGCCA 
CCGCCTCCTC CTCCTCCTCC TCCAGTTCCC GCCAAATTGT TCGGTGAATC GTTGGAGAAA 
GAGAAAAAGT CGGAAGACGA CACTGTCAAG CAAGAAACTA CTGGGGATTC TCCTGCTCCT 
CCCCCTCCAC CACCACCACC TCCTCCTCCA CCCATGGCAT TGTTCGGCAA ACCCAAGGGA 
GAAACACCAC CACCGCCGCC GCTACCATCT GTATTATCTT CATCTACTGA TGGCGTCATT 
CCGCCAGCTC CACCTATGAT GCCGGCATCG CAAATCAAAT CAGCTGTAAC ATCACCATTA 
CTTCCTCAAT CGCCCTCATT ATTTGAAAAG TATCCTCGTC CACACAAGAA ATTAAAGCAA 
CTGCATTGGG AAAAATTAGA TTGTACTGAT AATTCTATTT GGGGTACAGG TAAAGCTGAG 
AAGTTCGCAG ATGACTTATA CGAAAAGGGT GTTCTTGCTG ATTTAGAAAA GGCCTTTGCC 
GCTAGGGAAA TTAAATCATT AGCAAGCAAG CGCAAGGAAG ATCTGCAGAA AATTACATTT 
TTATCCAGGG ATATTTCTCA ACAGTTTGGT ATCAATTTGC ATATGTATTC ATCACTTTCG 
GTTGCCGATC TGGTGAAAAA GATTCTAAAC TGCGATAGGG ATTTTTTGCA AACACCGAGT 
GTTGTTGAGT TTTTGTCAAA ATCTGAAATA ATTGAAGTTT CTGTTAATTT AGCTAGGAAT 
TACGCCCCGT ATTCGACAGA CTGGGAGGGC GTGAGGAATC TCGAAGATGC CAAGCCCCCT 
GAAAAAGATC CAAACGATTT ACAGAGGGCT GACCAAATTT ATTTGCAATT GATGGTTAAT 
CTAGAATCAT ACTGGGGTTC ACGTATGAGA GCTCTCACAG TGGTTACTTC GTATGAAAGA 
GAATATAATG AGTTATTGGC AAAGTTAAGA AAAGTCGACA AAGCTGTTAG CGCGCTTCAA 
GAATCAGATA ACTTGCGCAA TGTCTTTAAT GTTATCTTAG CAGTTGGTAA TTTCATGAAC 
GACACTTCTA AGCAGGCACA AGGTTTCAAA CTTTCAACCT TGCAGAGGTT GACGTTTATC 
AAGGATACTA CCAATAGCAT GACCTTTCTA AATTACGTCG AAAAAATTGT CAGGTTAAAT 
TATCCAAGCT TCAATGACTT TCTAAGTGAA TTAGAACCTG TATTGGATGT CGTGAAAGTT 
TCCATTGAGC AGTTAGTTAA CGACTGTAAG GACTTTAGCC AATCGATTGT AAACGTAGAG 
CGTTCAGTTG AAATTGGTAA TTTAAGCGAT TCCTCAAAGT TTCATCCATT AGATAAAGTT 
TTGATAAAAA CATTGCCCGT GTTACCGGAA GCCAGAAAAA AGGGGGACTT GTTAGAAGAC 
GAAGTTAAGC TGACTATCAT GGAATTTGAA AGTCTGATGC ATACGTACGG TGAAGATTCT 
GGTGATAAGT TTGCAAAAAT CTCATTCTTT AAGAAATTTG CAGATTTTAT AAATGAATAC 
AAAAAAGCTC AAGCGCAAAA TCTAGCCGCG GAAGAAGAAG AAAGACTTTA TATAAAGCAT 
AAGAAAATAG TGGAAGAACA GCAAAAAAGG GCACAAGAAA AGGAAAAACA AAAAGAGAAC 
AGCAATTCGC CTTCATCTGA AGGAAATGAA GAGGATGAAG CAGAAGATAG GCGCGCTGTT 
ATGGATAAAC TATTAGAACA GTTGAAAAAT GCAGGTCCTG CTAAAAGTGA CCCATCTTCC 
GCCAGAAAGA GAGCCCTAGT GAGGAAAAAA TATCTTTCTG AAAAAGACAA CGCTCCACAA 
TTACTCAACG ATTTGGATAC TGAAGAAGGG TCTATTCTTT ATTCTCCAGA AGCTATGGAC 
CCTACTGCCG ATACAGTGAT TCACGCTGAA TCACCCACTC CGCTTGCCAC AAGGGGTGTT 
ATGAATACCT CTGAAGATTT ACCATCGCCA TCCAAGACAT CTGCGCTGGA AGATCAAGAG 
GAAATTAGTG ACAGAGCTAG AATGTTATTA AAGGAGCTAA GAGGCTCTGA CACACCAGTA 
AAGCAAAATT CCATACTCGA TGAACATCTA GAAAAATTGA GGGCCCGCAA GGAAAGATCC 
ATAGGTGAGG CTAGCACAGG TAACAGGCTA AGTTTCAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Nature387(6632 Suppl)93-8(1997 May)
Philippsen P,Kleine K,P?hlmann R,D?sterh?ft A,Hamberg K,Hegemann JH,Obermaier B,Urrestarazu LA,Aert R,Albermann K,Altmann R,Andr? B,Baladron V,Ballesta JP,B?cam AM,Beinhauer J,Boskovic J,Buitrago MJ,Bussereau F,Coster F,Crouzet M,D'Angelo M,Dal Pero F,De Antoni A,Del Rey F,Doignon F,Domdey H,Dubois E,Fiedler T,Fleig U,Floeth M,Fritz C,Gaillardin C,Garcia-Cantalejo JM,Glansdorff NN,Goffeau A,Gueldener U,Herbert C,Heumann K,Heuss-Neitzel D,Hilbert H,Hinni K,Iraqui Houssaini I,Jacquet M,Jimenez A,Jonniaux JL,Karpfinger L,Lanfranchi G,Lepingle A,Levesque H,Lyck R,Maftahi M,Mallet L,Maurer KC,Messenguy F,Mewes HW,M?sti D,Nasr F,Nicaud JM,Niedenthal RK,Pandolfo D,Pi?rard A,Piravandi E,Planta RJ,Pohl TM,Purnelle B,Rebischung C,Remacha M,Revuelta JL,Rinke M,Saiz JE,Sartorello F,Scherens B,Sen-Gupta M,Soler-Mira A,Urbanus JH,Valle G,Van Dyck L,Verhasselt P,Vierendeels F,Vissers S,Voet M,Volckaert G,Wach A,Wambutt R,Wedler H,Zollner A,Hani J

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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BNI1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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