NIP1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	NIP1
Entrez Gene ID	855357
Full Name	translation initiation factor eIF3 core subunit c
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182820.1	NP_014040.1	translation initiation factor eIF3 core subunit c

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	EIF3C	XP_536923.2
Bos taurus (cattle)	EIF3CL	NP_001029790.1
Mus musculus (house mouse)	Eif3c	NP_666312.1
Rattus norvegicus (Norway rat)	Eif3c	NP_001094132.1
Kluyveromyces lactis	KLLA0F03014g	XP_455216.1
Schizosaccharomyces pombe (fission yeast)	tif33	NP_593828.2
Arabidopsis thaliana (thale cress)	EIF3C	NP_001190102.1
Xenopus tropicalis (tropical clawed frog)	eif3c	NP_989413.1
Homo sapiens (human)	EIF3C	NP_003743.1
Saccharomyces cerevisiae (baker's yeast)	NIP1	NP_014040.1
Danio rerio (zebrafish)	eif3c	NP_998628.1
Drosophila melanogaster (fruit fly)	eIF3-S8	NP_611242.1
Eremothecium gossypii	AGOS_AGL344C	NP_986323.1
Arabidopsis thaliana (thale cress)	TIF3C2	NP_188926.4
Oryza sativa (rice)	Os07g0124500	NP_001058804.1
Magnaporthe oryzae (rice blast fungus)	MGG_16895	XP_003712797.1
	LOC705696	XP_002808338.1
Anopheles gambiae (African malaria mosquito)	EIF3C_ANOGA	XP_318103.3
Caenorhabditis elegans (roundworm)	eif-3.C	NP_492638.1
Neurospora crassa	NCU07831	XP_962952.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Bowman S, Churcher C, Badcock K, Brown D, Chillingworth T, Connor R, Dedman K, Devlin K, Gentles S, Hamlin N, Hunt S, Jagels K, Lye G, Moule S, Odell C, Pearson D, Rajandream M, Rice P, Skelton J, Walsh S, Whitehead S, Barrell B
Nature387(6632 Suppl)90-3(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

binding of eIF1 to the c/Nip1-NTD is equally important for its initial recruitment to PICs and for its proper functioning in selecting the translational start site
Title: Functional characterization of the role of the N-terminal domain of the c/Nip1 subunit of eukaryotic initiation factor 3 (eIF3) in AUG recognition.

the NIP1-N-terminal domain is required for efficient assembly of preinitiation complexes and also regulates the selection of AUG start codons in vivo
Title: Interactions of eukaryotic translation initiation factor 3 (eIF3) subunit NIP1/c with eIF1 and eIF5 promote preinitiation complex assembly and regulate start codon selection.

The following NIP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NIP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04302	NM_001182820.1 Latest version!	Saccharomyces cerevisiae S288C translation initiation factor eIF3 core subunit c (NIP1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NIP1 ( NM_001182820.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_014040.1 Saccharomyces cerevisiae S288C translation initiation factor eIF3 core subunit c (NIP1), partial mRNA.

Price & Availability↑

CloneID	OSi04302
Clone ID Related Accession (Same CDS sequence)	NM_001182820.1
Accession Version	NM_001182820.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2439bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	translation initiation factor eIF3 core subunit c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001145). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCCCGTT TCTTTTCGTC TAATTACGAA TACGATGTAG CCAGTTCTTC ATCCGAAGAA 
GATCTTTTAT CTTCGTCTGA AGAAGATTTG TTAAGCTCTT CCTCCTCTGA GTCTGAATTG 
GACCAAGAAT CTGACGACTC CTTTTTCAAT GAAAGTGAAA GTGAAAGTGA AGCTGATGTA 
GACTCTGATG ATTCTGATGC AAAGCCTTAT GGTCCTGACT GGTTCAAGAA ATCTGAGTTC 
AGAAAACAAG GTGGAGGTTC AAATAAATTT TTGAAAAGCT CTAACTATGA TTCCAGTGAT 
GAAGAATCCG ATGAAGAAGA TGGCAAGAAG GTAGTCAAGT CTGCCAAAGA AAAACTATTG 
GATGAAATGC AAGACGTTTA TAATAAGATC TCTCAAGCTG AGAACTCTGA TGACTGGTTG 
ACTATTTCTA ATGAGTTTGA TTTGATCTCG CGTCTCTTAG TTAGGGCTCA ACAACAAAAC 
TGGGGGACTC CAAATATTTT CATCAAGGTT GTTGCCCAAG TGGAGGACGC TGTGAATAAT 
ACACAACAAG CTGATTTGAA GAATAAAGCT GTTGCAAGAG CTTATAACAC TACAAAGCAA 
AGAGTCAAGA AAGTTTCTAG AGAAAATGAA GACTCAATGG CTAAATTCAG AAATGATCCT 
GAATCATTTG ATAAGGAACC AACCGCAGAT TTGGATATTT CTGCTAATGG ATTCACAATT 
TCTTCGTCTC AAGGCAATGA CCAAGCGGTA CAAGAAGATT TCTTCACTAG ATTACAAACA 
ATAATTGACT CAAGAGGTAA GAAGACTGTC AATCAACAAT CCTTGATTTC TACTTTGGAG 
GAGTTATTAA CTGTAGCTGA AAAACCATAT GAATTCATAA TGGCTTATTT GACTTTGATT 
CCATCAAGAT TCGATGCCTC AGCTAACCTA TCTTACCAAC CAATTGATCA ATGGAAATCT 
TCATTCAACG ATATTAGTAA ATTATTGTCT ATTTTAGACC AGACAATTGA CACCTACCAA 
GTTAATGAAT TTGCTGATCC AATCGATTTC ATTGAAGATG AACCTAAAGA AGATTCTGAT 
GGTGTCAAGA GGATTCTGGG TTCCATTTTC TCATTTGTTG AAAGATTAGA TGACGAATTC 
ATGAAATCCC TGTTAAACAT CGATCCTCAT TCCAGTGATT ATTTGATCCG TTTAAGGGAT 
GAACAATCAA TCTATAATTT GATCCTAAGA ACTCAATTGT ACTTTGAAGC GACTTTGAAA 
GATGAACACG ACCTAGAAAG AGCATTGACA CGTCCATTCG TCAAGAGATT GGATCATATC 
TACTATAAAT CCGAAAATTT GATAAAAATT ATGGAAACTG CTGCTTGGAA TATCATACCT 
GCTCAATTCA AATCTAAATT TACTTCAAAA GACCAGCTCG ATTCTGCTGA TTATGTCGAC 
AATTTAATAG ACGGATTATC GACAATCTTA TCCAAGCAAA ACAACATTGC TGTTCAAAAA 
CGTGCTATTT TATACAACAT TTACTACACT GCATTAAACA AAGATTTCCA AACTGCTAAA 
GATATGTTAC TAACTTCCCA AGTTCAAACA AATATCAACC AATTCGATTC ATCCCTACAA 
ATTTTATTCA ACAGGGTTGT TGTTCAATTG GGTCTATCCG CCTTTAAATT ATGTTTGATT 
GAAGAATGTC ATCAAATTTT GAATGATCTT CTGTCAAGTT CTCACTTAAG AGAAATTTTG 
GGCCAACAAT CCCTACACAG AATATCTCTC AATTCTAGTA ACAATGCTTC AGCTGATGAG 
CGTGCTAGAC AATGTTTGCC ATATCACCAA CACATCAATC TCGATTTAAT CGATGTCGTC 
TTCTTAACAT GTTCCTTATT GATCGAAATT CCAAGAATGA CTGCCTTCTA TTCCGGTATT 
AAGGTCAAGA GAATTCCTTA CTCTCCAAAA TCCATTCGTC GTTCCTTAGA ACATTACGAC 
AAGTTAAGTT TCCAAGGTCC ACCAGAAACT TTAAGAGATT ATGTCTTGTT TGCTGCCAAA 
TCAATGCAAA AAGGTAACTG GAGAGACTCT GTTAAATACT TAAGAGAAAT AAAATCTTGG 
GCTTTATTAC CAAACATGGA AACGGTGTTG AATAGTTTAA CGGAAAGAGT ACAAGTTGAA 
TCTTTGAAGA CTTATTTCTT TTCTTTCAAG AGGTTCTATT CAAGTTTTTC TGTTGCTAAA 
CTAGCCGAAT TATTTGATCT TCCAGAAAAT AAGGTGGTTG AAGTTTTGCA ATCTGTTATC 
GCAGAATTGG AAATCCCAGC CAAATTAAAC GACGAGAAGA CCATCTTTGT TGTCGAAAAG 
GGTGATGAAA TTACTAAATT GGAAGAAGCA ATGGTAAAAT TGAACAAAGA ATATAAAATC 
GCTAAAGAAC GTCTTAACCC ACCATCAAAT CGTCGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_014040.1
CDS	1..2439
Translation	MSRFFSSNYEYDVASSSSEEDLLSSSEEDLLSSSSSESELDQESDDSFFNESESESEADVDSDDSDAKPYGPDWFKKSEFRKQGGGSNKFLKSSNYDSSDEESDEEDGKKVVKSAKEKLLDEMQDVYNKISQAENSDDWLTISNEFDLISRLLVRAQQQNWGTPNIFIKVVAQVEDAVNNTQQADLKNKAVARAYNTTKQRVKKVSRENEDSMAKFRNDPESFDKEPTADLDISANGFTISSSQGNDQAVQEDFFTRLQTIIDSRGKKTVNQQSLISTLEELLTVAEKPYEFIMAYLTLIPSRFDASANLSYQPIDQWKSSFNDISKLLSILDQTIDTYQVNEFADPIDFIEDEPKEDSDGVKRILGSIFSFVERLDDEFMKSLLNIDPHSSDYLIRLRDEQSIYNLILRTQLYFEATLKDEHDLERALTRPFVKRLDHIYYKSENLIKIMETAAWNIIPAQFKSKFTSKDQLDSADYVDNLIDGLSTILSKQNNIAVQKRAILYNIYYTALNKDFQTAKDMLLTSQVQTNINQFDSSLQILFNRVVVQLGLSAFKLCLIEECHQILNDLLSSSHLREILGQQSLHRISLNSSNNASADERARQCLPYHQHINLDLIDVVFLTCSLLIEIPRMTAFYSGIKVKRIPYSPKSIRRSLEHYDKLSFQGPPETLRDYVLFAAKSMQKGNWRDSVKYLREIKSWALLPNMETVLNSLTERVQVESLKTYFFSFKRFYSSFSVAKLAELFDLPENKVVEVLQSVIAELEIPAKLNDEKTIFVVEKGDEITKLEEAMVKLNKEYKIAKERLNPPSNRR

Target ORF information:

RefSeq Version	NM_001182820.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C translation initiation factor eIF3 core subunit c (NIP1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182820.1

ORF Insert Sequence:

ATGTCCCGTT TCTTTTCGTC TAATTACGAA TACGATGTAG CCAGTTCTTC ATCCGAAGAA 
GATCTTTTAT CTTCGTCTGA AGAAGATTTG TTAAGCTCTT CCTCCTCTGA GTCTGAATTG 
GACCAAGAAT CTGACGACTC CTTTTTCAAT GAAAGTGAAA GTGAAAGTGA AGCTGATGTA 
GACTCTGATG ATTCTGATGC AAAGCCTTAT GGTCCTGACT GGTTCAAGAA ATCTGAGTTC 
AGAAAACAAG GTGGAGGTTC AAATAAATTT TTGAAAAGCT CTAACTATGA TTCCAGTGAT 
GAAGAATCCG ATGAAGAAGA TGGCAAGAAG GTAGTCAAGT CTGCCAAAGA AAAACTATTG 
GATGAAATGC AAGACGTTTA TAATAAGATC TCTCAAGCTG AGAACTCTGA TGACTGGTTG 
ACTATTTCTA ATGAGTTTGA TTTGATCTCG CGTCTCTTAG TTAGGGCTCA ACAACAAAAC 
TGGGGGACTC CAAATATTTT CATCAAGGTT GTTGCCCAAG TGGAGGACGC TGTGAATAAT 
ACACAACAAG CTGATTTGAA GAATAAAGCT GTTGCAAGAG CTTATAACAC TACAAAGCAA 
AGAGTCAAGA AAGTTTCTAG AGAAAATGAA GACTCAATGG CTAAATTCAG AAATGATCCT 
GAATCATTTG ATAAGGAACC AACCGCAGAT TTGGATATTT CTGCTAATGG ATTCACAATT 
TCTTCGTCTC AAGGCAATGA CCAAGCGGTA CAAGAAGATT TCTTCACTAG ATTACAAACA 
ATAATTGACT CAAGAGGTAA GAAGACTGTC AATCAACAAT CCTTGATTTC TACTTTGGAG 
GAGTTATTAA CTGTAGCTGA AAAACCATAT GAATTCATAA TGGCTTATTT GACTTTGATT 
CCATCAAGAT TCGATGCCTC AGCTAACCTA TCTTACCAAC CAATTGATCA ATGGAAATCT 
TCATTCAACG ATATTAGTAA ATTATTGTCT ATTTTAGACC AGACAATTGA CACCTACCAA 
GTTAATGAAT TTGCTGATCC AATCGATTTC ATTGAAGATG AACCTAAAGA AGATTCTGAT 
GGTGTCAAGA GGATTCTGGG TTCCATTTTC TCATTTGTTG AAAGATTAGA TGACGAATTC 
ATGAAATCCC TGTTAAACAT CGATCCTCAT TCCAGTGATT ATTTGATCCG TTTAAGGGAT 
GAACAATCAA TCTATAATTT GATCCTAAGA ACTCAATTGT ACTTTGAAGC GACTTTGAAA 
GATGAACACG ACCTAGAAAG AGCATTGACA CGTCCATTCG TCAAGAGATT GGATCATATC 
TACTATAAAT CCGAAAATTT GATAAAAATT ATGGAAACTG CTGCTTGGAA TATCATACCT 
GCTCAATTCA AATCTAAATT TACTTCAAAA GACCAGCTCG ATTCTGCTGA TTATGTCGAC 
AATTTAATAG ACGGATTATC GACAATCTTA TCCAAGCAAA ACAACATTGC TGTTCAAAAA 
CGTGCTATTT TATACAACAT TTACTACACT GCATTAAACA AAGATTTCCA AACTGCTAAA 
GATATGTTAC TAACTTCCCA AGTTCAAACA AATATCAACC AATTCGATTC ATCCCTACAA 
ATTTTATTCA ACAGGGTTGT TGTTCAATTG GGTCTATCCG CCTTTAAATT ATGTTTGATT 
GAAGAATGTC ATCAAATTTT GAATGATCTT CTGTCAAGTT CTCACTTAAG AGAAATTTTG 
GGCCAACAAT CCCTACACAG AATATCTCTC AATTCTAGTA ACAATGCTTC AGCTGATGAG 
CGTGCTAGAC AATGTTTGCC ATATCACCAA CACATCAATC TCGATTTAAT CGATGTCGTC 
TTCTTAACAT GTTCCTTATT GATCGAAATT CCAAGAATGA CTGCCTTCTA TTCCGGTATT 
AAGGTCAAGA GAATTCCTTA CTCTCCAAAA TCCATTCGTC GTTCCTTAGA ACATTACGAC 
AAGTTAAGTT TCCAAGGTCC ACCAGAAACT TTAAGAGATT ATGTCTTGTT TGCTGCCAAA 
TCAATGCAAA AAGGTAACTG GAGAGACTCT GTTAAATACT TAAGAGAAAT AAAATCTTGG 
GCTTTATTAC CAAACATGGA AACGGTGTTG AATAGTTTAA CGGAAAGAGT ACAAGTTGAA 
TCTTTGAAGA CTTATTTCTT TTCTTTCAAG AGGTTCTATT CAAGTTTTTC TGTTGCTAAA 
CTAGCCGAAT TATTTGATCT TCCAGAAAAT AAGGTGGTTG AAGTTTTGCA ATCTGTTATC 
GCAGAATTGG AAATCCCAGC CAAATTAAAC GACGAGAAGA CCATCTTTGT TGTCGAAAAG 
GGTGATGAAA TTACTAAATT GGAAGAAGCA ATGGTAAAAT TGAACAAAGA ATATAAAATC 
GCTAAAGAAC GTCTTAACCC ACCATCAAAT CGTCGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Nature387(6632 Suppl)90-3(1997 May)
Bowman S,Churcher C,Badcock K,Brown D,Chillingworth T,Connor R,Dedman K,Devlin K,Gentles S,Hamlin N,Hunt S,Jagels K,Lye G,Moule S,Odell C,Pearson D,Rajandream M,Rice P,Skelton J,Walsh S,Whitehead S,Barrell B

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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NIP1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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