PRP24 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	PRP24
Entrez Gene ID	855310
Full Name	U6 snRNP complex subunit PRP24
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182775.1	NP_013995.1	U6 snRNP complex subunit PRP24

Pathways from BioSystems

Homologies

Neurospora crassa	NCU09048	XP_958552.1
Saccharomyces cerevisiae (baker's yeast)	PRP24	NP_013995.1
Schizosaccharomyces pombe (fission yeast)	SPBC1861.04c	NP_596721.1
Kluyveromyces lactis	KLLA0E11045g	XP_454447.1
Magnaporthe oryzae (rice blast fungus)	MGG_04727	XP_003710812.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Bowman S, Churcher C, Badcock K, Brown D, Chillingworth T, Connor R, Dedman K, Devlin K, Gentles S, Hamlin N, Hunt S, Jagels K, Lye G, Moule S, Odell C, Pearson D, Rajandream M, Rice P, Skelton J, Walsh S, Whitehead S, Barrell B
Nature387(6632 Suppl)90-3(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that U6 snRNP complex subunit Prp24 binds U6 snRNA with much higher affinity than U4 snRNA.
Title: Structural requirements for protein-catalyzed annealing of U4 and U6 RNAs during di-snRNP assembly.

results suggest that the autophagy and proteasomal pathways degrade distinct translation and RNA turnover factors in a Ubp3p-dependent manner during nitrogen starvation.
Title: Spliceosome assembly in the absence of stable U4/U6 RNA pairing.

The crystal structure of the Saccharomyces cerevisiae U6 snRNP core containing most of the U6 snRNA and all four RRM domains of the Prp24 protein is presented.
Title: Core structure of the U6 small nuclear ribonucleoprotein at 1.7-? resolution.

Nearly complete backbone and side chain resonance assignments have been obtained for the second RNA recognition motif (RRM) of Prp24 (RRM2, residues M114-E197) both in isolation and bound to a six nucleotide fragment of U6 RNA (AGAGAU).
Title: (1)H, (13)C and (15)N resonance assignments of a ribonucleoprotein complex consisting of Prp24-RRM2 bound to a fragment of U6 RNA.

structure of the RNA recognition motifs of splicing factor prp24.
Title: Structure and interactions of the first three RNA recognition motifs of splicing factor prp24.

The following PRP24 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRP24 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04261	NM_001182775.1 Latest version!	Saccharomyces cerevisiae S288C U6 snRNP complex subunit PRP24 (PRP24), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRP24 ( NM_001182775.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013995.1 Saccharomyces cerevisiae S288C U6 snRNP complex subunit PRP24 (PRP24), partial mRNA.

Price & Availability↑

CloneID	OSi04261
Clone ID Related Accession (Same CDS sequence)	NM_001182775.1
Accession Version	NM_001182775.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1335bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	U6 snRNP complex subunit PRP24
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001145). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGAGTATG GACATCACGC TAGACCAGAT TCAAAACGAC CATTGGATGA AGGATCACCA 
GCAGCCGCAG GATTAACTTC TAAGAAGGCG AACGAAGCTT TAACAAGAAA TAGAGAATTA 
ACAACTGTAT TAGTCAAAAA TTTGCCTAAG AGCTATAATC AAAACAAAGT CTATAAGTAC 
TTCAAACATT GTGGGCCCAT TATACACGTT GATGTTGCGG ATTCGCTGAA GAAGAACTTT 
CGTTTTGCAC GTATTGAATT TGCCAGGTAT GATGGAGCCC TCGCTGCAAT AACCAAAACA 
CACAAAGTTG TAGGTCAGAA TGAAATTATA GTATCTCATT TAACAGAATG CACATTATGG 
ATGACGAATT TCCCCCCAAG TTATACTCAA AGAAATATTA GAGATCTATT GCAAGATATT 
AACGTTGTTG CACTCAGTAT ACGGCTTCCC AGTTTACGAT TCAATACAAG CAGAAGGTTC 
GCTTACATCG ATGTTACTTC TAAAGAGGAT GCAAGATATT GTGTAGAAAA ATTGAATGGA 
CTCAAAATAG AAGGTTATAC TTTAGTTACT AAAGTTTCCA ATCCGCTGGA AAAGTCGAAA 
CGGACCGATT CTGCCACACT AGAGGGGCGA GAGATTATGA TACGAAACCT AAGTACAGAA 
TTGCTCGACG AAAACCTTCT GAGGGAATCT TTTGAGGGAT TTGGTTCCAT TGAAAAAATC 
AACATACCTG CTGGACAGAA AGAGCACAGT TTCAATAACT GTTGTGCATT TATGGTTTTT 
GAAAATAAAG ATTCTGCTGA AAGGGCACTT CAAATGAATA GAAGTTTGTT AGGTAATAGG 
GAAATTTCCG TTTCCTTGGC TGATAAAAAA CCATTCTTAG AAAGAAACGA AGTCAAAAGA 
CTTCTCGCAT CGCGTAACAG CAAAGAACTA GAAACACTAA TATGCTTATT TCCACTTTCA 
GATAAGGTGT CACCAAGCTT AATCTGCCAA TTTCTTCAGG AAGAAATACA CATCAATGAA 
AAAGATATTA GGAAAATACT TCTCGTTAGC GACTTCAATG GCGCCATTAT TATATTTAGA 
GATAGTAAGT TTGCAGCAAA AATGCTAATG ATACTAAATG GATCTCAATT TCAGGGAAAG 
GTGATACGTT CAGGTACTAT TAATGACATG AAAAGATATT ATAACAATCA ACAGAATCAC 
AGCATGAAAC ACGTCAAACC TAGTTGTATA AACATGATGG AAAAAGGACC AAATCTTCAA 
GTAAAGAAAA AAATTCCTGA CAAACAAGAG CAGATGTCCA ACGACGATTT TCGCAAGATG 
TTTCTAGGTG AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013995.1
CDS	1..1335
Translation	MEYGHHARPDSKRPLDEGSPAAAGLTSKKANEALTRNRELTTVLVKNLPKSYNQNKVYKYFKHCGPIIHVDVADSLKKNFRFARIEFARYDGALAAITKTHKVVGQNEIIVSHLTECTLWMTNFPPSYTQRNIRDLLQDINVVALSIRLPSLRFNTSRRFAYIDVTSKEDARYCVEKLNGLKIEGYTLVTKVSNPLEKSKRTDSATLEGREIMIRNLSTELLDENLLRESFEGFGSIEKINIPAGQKEHSFNNCCAFMVFENKDSAERALQMNRSLLGNREISVSLADKKPFLERNEVKRLLASRNSKELETLICLFPLSDKVSPSLICQFLQEEIHINEKDIRKILLVSDFNGAIIIFRDSKFAAKMLMILNGSQFQGKVIRSGTINDMKRYYNNQQNHSMKHVKPSCINMMEKGPNLQVKKKIPDKQEQMSNDDFRKMFLGE

Target ORF information:

RefSeq Version	NM_001182775.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C U6 snRNP complex subunit PRP24 (PRP24), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182775.1

ORF Insert Sequence:

ATGGAGTATG GACATCACGC TAGACCAGAT TCAAAACGAC CATTGGATGA AGGATCACCA 
GCAGCCGCAG GATTAACTTC TAAGAAGGCG AACGAAGCTT TAACAAGAAA TAGAGAATTA 
ACAACTGTAT TAGTCAAAAA TTTGCCTAAG AGCTATAATC AAAACAAAGT CTATAAGTAC 
TTCAAACATT GTGGGCCCAT TATACACGTT GATGTTGCGG ATTCGCTGAA GAAGAACTTT 
CGTTTTGCAC GTATTGAATT TGCCAGGTAT GATGGAGCCC TCGCTGCAAT AACCAAAACA 
CACAAAGTTG TAGGTCAGAA TGAAATTATA GTATCTCATT TAACAGAATG CACATTATGG 
ATGACGAATT TCCCCCCAAG TTATACTCAA AGAAATATTA GAGATCTATT GCAAGATATT 
AACGTTGTTG CACTCAGTAT ACGGCTTCCC AGTTTACGAT TCAATACAAG CAGAAGGTTC 
GCTTACATCG ATGTTACTTC TAAAGAGGAT GCAAGATATT GTGTAGAAAA ATTGAATGGA 
CTCAAAATAG AAGGTTATAC TTTAGTTACT AAAGTTTCCA ATCCGCTGGA AAAGTCGAAA 
CGGACCGATT CTGCCACACT AGAGGGGCGA GAGATTATGA TACGAAACCT AAGTACAGAA 
TTGCTCGACG AAAACCTTCT GAGGGAATCT TTTGAGGGAT TTGGTTCCAT TGAAAAAATC 
AACATACCTG CTGGACAGAA AGAGCACAGT TTCAATAACT GTTGTGCATT TATGGTTTTT 
GAAAATAAAG ATTCTGCTGA AAGGGCACTT CAAATGAATA GAAGTTTGTT AGGTAATAGG 
GAAATTTCCG TTTCCTTGGC TGATAAAAAA CCATTCTTAG AAAGAAACGA AGTCAAAAGA 
CTTCTCGCAT CGCGTAACAG CAAAGAACTA GAAACACTAA TATGCTTATT TCCACTTTCA 
GATAAGGTGT CACCAAGCTT AATCTGCCAA TTTCTTCAGG AAGAAATACA CATCAATGAA 
AAAGATATTA GGAAAATACT TCTCGTTAGC GACTTCAATG GCGCCATTAT TATATTTAGA 
GATAGTAAGT TTGCAGCAAA AATGCTAATG ATACTAAATG GATCTCAATT TCAGGGAAAG 
GTGATACGTT CAGGTACTAT TAATGACATG AAAAGATATT ATAACAATCA ACAGAATCAC 
AGCATGAAAC ACGTCAAACC TAGTTGTATA AACATGATGG AAAAAGGACC AAATCTTCAA 
GTAAAGAAAA AAATTCCTGA CAAACAAGAG CAGATGTCCA ACGACGATTT TCGCAAGATG 
TTTCTAGGTG AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Nature387(6632 Suppl)90-3(1997 May)
Bowman S,Churcher C,Badcock K,Brown D,Chillingworth T,Connor R,Dedman K,Devlin K,Gentles S,Hamlin N,Hunt S,Jagels K,Lye G,Moule S,Odell C,Pearson D,Rajandream M,Rice P,Skelton J,Walsh S,Whitehead S,Barrell B

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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PRP24 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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