RKR1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	RKR1
Entrez Gene ID	855289
Full Name	ubiquitin-protein ligase RKR1
Synonyms	LTN1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182755.1	NP_013975.1	ubiquitin-protein ligase RKR1

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0C14344g	XP_452842.1
Eremothecium gossypii	AGOS_ABL058C	NP_982889.2
Saccharomyces cerevisiae (baker's yeast)	RKR1	NP_013975.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Bowman S, Churcher C, Badcock K, Brown D, Chillingworth T, Connor R, Dedman K, Devlin K, Gentles S, Hamlin N, Hunt S, Jagels K, Lye G, Moule S, Odell C, Pearson D, Rajandream M, Rice P, Skelton J, Walsh S, Whitehead S, Barrell B
Nature387(6632 Suppl)90-3(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that Saccharomyces cerevisiae proteins Rqc1, Ltn1, and Cdc48 prevent the aggregation of aberrant proteins that can only appear after C-terminal alanine-threonine tail (CAT-tail) addition by Rqc2.
Title: Rqc1 and Ltn1 Prevent C-terminal Alanine-Threonine Tail (CAT-tail)-induced Protein Aggregation by Efficient Recruitment of Cdc48 on Stalled 60S Subunits.

findings can explain the proteotoxicity of ribosome-stalled polypeptides and demonstrate the essential role of the RQC in maintaining proteostasis
Title: Failure of RQC machinery causes protein aggregation and proteotoxic stress.

Our results indicate that Rkr1 plays the primary role in targeting the tested model ER-targeted nonstop and translationally stalled proteins for degradation
Title: Rkr1/Ltn1 Ubiquitin Ligase-mediated Degradation of Translationally Stalled Endoplasmic Reticulum Proteins.

Ltn1 and Ubp3-Bre5 likely contribute to adapt ribophagy activity to both nutrient supply and protein translation.
Title: Ubiquitylation by the Ltn1 E3 ligase protects 60S ribosomes from starvation-induced selective autophagy.

Data show that Ltn1 E3 ligase has an elongated form and presents a continuum of conformational states about two flexible hinge regions.
Title: Single-particle EM reveals extensive conformational variability of the Ltn1 E3 ligase.

The following RKR1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RKR1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04242	NM_001182755.1 Latest version!	Saccharomyces cerevisiae S288C ubiquitin-protein ligase RKR1 (RKR1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RKR1 ( NM_001182755.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013975.1 Saccharomyces cerevisiae S288C ubiquitin-protein ligase RKR1 (RKR1), partial mRNA.

Price & Availability↑

CloneID	OSi04242
Clone ID Related Accession (Same CDS sequence)	NM_001182755.1
Accession Version	NM_001182755.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4689bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	ubiquitin-protein ligase RKR1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001145). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCGTTTG GTGGAATCAA TACATTTCAA CAGTATAACA CAGATTTAGG GCTCGGCCAT 
AATGGTGTTA GAATATCCTT AAACTACTTT GATGGTTTGC CGGATCCAAG TCTTTTAAAC 
TCCTTGTACT CGAACGAGCT GAAACTCATT TTCAAATCTC TTCTGAAAAG AGATGAAACT 
ACAAAAGAAA AAGCTTTAAT GGATCTTTCC AATTTGATCA GCGACTTTAA CCAGAATGAG 
TACTTTTTCA ATGACATTTT TCTTCTTTGT TGGTCGCAGA TATATGCTAA ACTGATAATT 
AGTGATTACA AGGTCATCAG ACTGCAATCA CATCAAATTA CTATAATGCT TGTCAAAAGC 
CTTCGGAAGA AAATATCGAA GTTTTTAAAG GATTTTATAC CTTTGATACT TCTCGGTACA 
TGCGAACTCG ATTATAGTGT TTCCAAACCT TCTCTTAATG AATTAACAGA GTGTTTCAAC 
AAAGACCCAG CAAAAATTAA CGCCTTATGG GCTGTTTTTC AAGAACAACT ATTGAATTTA 
GTAAAGGAGA TTGTTGTAAA CGAGAATGAA GATACCATTT CTGATGAACG TTACTCCAGT 
AAAGAAGAAT CTGAGTTTAG ATACCACAGA GTTATAGCAA GTGCCGTATT GCTGCTGATT 
AAGCTTTTTG TTCATAACAA AGACGTCTCA GAACGTAATT CTTCCAGTCT TAAGGTGATT 
CTTTCTGATG AGTCAATCTG GAAACTACTT AACTTAAAAA ATGGGCAAAA TACAAATGCC 
TACGAAACAG TTCTTCGATT GATTGATGTT CTTTATACAA GAGGTTACAT GCCTTCCCAC 
AAAAATATAA TGAAGTTAGC GGTGAAAAAA CTATTGAAAT CGTTGACTCA TATTACGTCA 
AAGAATATTC TAAAAGTATG CCCTGTTCTC CCATCAATAC TGAACCTTTT AGCTACCTTG 
GATGACTATG AAGATGGGAC AATATGGTCA TACGATAAAT CTTCTAAAGA AAAAGTACTC 
AAATTTTTAT CGGTTTCCCG TACTTCACCA TCGCCAGGAT TTTTCAATGC TGTGTTTGCA 
TTGTACAGCT CGACTAAAAG ACACAGTTTT CTTGATTATT ATCTTGAATG GCTTCCATTT 
TGGCAAAAAT CGGTGCAAAG ACTGAATGAA AAGGGCTTTT CTGCGAGAAA CAGCGCTGAG 
GTTCTTAATG AGTTTTGGAC AAATTTTTTG AAGTTCGCTG AGGATTCATC AGAAGAAAGA 
GTGAAAAAAA TGGTGGAAAG TGAGATTTTT AACTCTCTGT CTTGCGGGAA ATCTTTATCT 
GAGTACACAA AACTGAACCA GACTCTTAGT GGCGTCTTTC CACCCGATAA ATGGGAGAGA 
GAGATTGAAG ATTACTTCAC TTCAGACGAA GACATAAGAA AAATAAAGGT GAGCTTTGAG 
AAGAATCTTT TTGCTTTATT GGTTACTTCT CCTAATAACG AATCCGCAAT TAGTCGTTTA 
TTCGATTTTT TTGTGCAATT AATCGAAACA GATCCCTCAA ATGTTTTCAA CAAATACGAC 
GGTGTATATG ATGCTCTCAA TTACTTTTTA GATTCAGATA TGATTTTCTT GAATGGGAAG 
ATAGGGAAAT TTATAAACGA AATTCCAACG TTGGTCCAAG AATCAACATA TCAAAACTTT 
GCTGGTATCA TGGCACAGTA CTCAAACTCC AAATTTTTCA AAATGAATAC TGACGCGATT 
ACTTCGTTGG AAGACTTTTT TATCGTTGCG CTTTCTTTTA ACTTACCAAA GACAATAATT 
TTAGCCACAA TGAATGAGTT GGACAATGAT ATTTATCAAC AATTAATGAA ATCAGATTCC 
CTAGAATTAG AATTGTACAT TGAAGACTTT ATGAAAAATT ATAAGTTTGA CGACAGTGGG 
GAAATATTTA AAGGTAATAA TAAATTTTTA AATCAAAGGA CTATCACCAC ACTTTACCGA 
AGCGCAGTTG CCAATGGCCA AGTTGAACAA TTTTGTGCTG TTTTGTCTAA ATTAGATGAA 
ACTTTTTTTT CAACGTTATT GCTGAATACC GATTTTCTTT CTTGTGCATT GTATGAAGTT 
TCAGAAGACA CTAATGAGAA ACTATTCAAG TTGTCATTAC AGTTGGCTAA AGGGAATTCT 
GAAATCGCTA ATAAGTTGGC CCAAGTGATT TTGCAACATG CTCAGGTTTA TTTCAGCCCC 
GGGGCAAAAG AAAAATATGT CACACACGCC GTGGAGCTAA TTAATGGCTG CAACGATACT 
TCTCAGATTT TTTTTCCCGC TAACGCAATC GAAGTTTTTG CCAGATATAT GCCAGCAATT 
GATTATAGGT CATCCTTAGT GAGCAGCTTA AGTACTAACA CGCATTTGCT CCTTACAGAT 
GATAAGCCTA TCAATTTAAA AAATATGCAA AAGCTCATCA GGTATGCTCT ATTCTTAGAT 
GCCCTACTTG ATGCTCTTCC AGAGCGCGTT AATAACCATA TTGTTGCATT TATAACTGTA 
GTTTCTGAGC TCGTCACTGA TTATAATTGC CTTTCAGAGG AACCAAATGA TCTTTACTAT 
GATTTTGGGC ATACCTTTTT CAAACATGGC AAAGTAAATT TGAACTTTTC TGATATTGTT 
GGAAACGTTA TTCAGCCTGC TAATGGTGGT GATGCTATGC TTACTTTTGA TATTGCTGAA 
AGTAATTCTG TCTACTTTTT TTATTATTCA AGAGTATTAT ATAAGGTTCT ATTGAACAGC 
ATTGATACTG TTTCATCCAC CACATTAAAT GGGCTATTAG CATCGGTTGA AAGTTTTGTC 
ACGAAAACTG TGAGGGATCA AAAATCTACT GATAAAGATT ATCTCCTATG TGCTATATTA 
CTATTGATGT TTAACAGGTC CAACTCAAAA GATGAGATAA CGAAATTAAG GACATTGCTG 
GCATCTCAAT TGATTGGGAT TAGAGAAGTA GAACTTGTCG ATCAAGAATT CAAGTCGTTA 
GCTCTTTTAA ATAATCTGCT GGATATCCCA CAGGCAGATA AACAGTTTGT TCCTATAGCA 
CCGCAGAGAC TGAACATGAT ATTCCGTTCA ATACTAAAAT GGTTGGATAG TGATCTTGCC 
TACGAGCCTA GCTTTAGCAC CGTTAGATTG TTACTACTAG ATTTTTTCAC CAAACTGATG 
AGGTTTGAAG GAGTCAGAGA TATGGGCATA ACAGCTTTTG AACTTTCGGA AAGGTTGCTG 
GCAGACTCTC TATCGATGTG TCAAATTGAT GACACTTTAT ATTTGTTAGA ACTTCGCTCT 
TCCTGTTTGA ACTTATATGA AACATTGTCG CAAGGCGTTT CCAAAAATGG CGAGGAAATC 
TCAGAATATG GTGATGAAAT TCAAGAAAAT TTGATTGAAC TGATGTTTCT CAACTTTAAT 
CAAGAAAGAA ATAACCAAGT ATCCACACTC TTCTACCAGA AATTGTACAA GGTCATTTCC 
TCAATGGAAC TCAAAAAGTT AGAATCTCAA TACAAAAGAA TATTCGAGGT TGTATTGAAT 
GATAAGGATA TCGGTAGTAA TATTAATCAA TCAAGATTAT TAACTACTTT GTTGGGTTCT 
TTGGTGGTAA AAACACAGCA GGATATTATA ATTGAGTATG AACTGAGGAT TCAAAAACAA 
ACTGGCTCAG ACGTAGATGG TAGTGCATCT GATAATGATG TAAACTCAAA ATTTAAACTA 
CCTCAAAAAT TATTACAAAA AGTTACTGAT GAGGTGCCAA AAGAATACCT TGAATACGAG 
AATAAAAATT CATTTATTAA ATATCTATGG TATTGGCACT TGATACTGAT GTACTTCAAA 
GATACGTCTT ATAATATGAG ACAAATATTT ATTGAACAAT TAAAGGAAGC TGGTTTAATC 
AATAGAATGT TTGATTTCAT AACAGATCAA ATAGATTTAA GAGATACTGA GTTTTGGAAA 
CAGGTCGACA CCAAGGAAAT TTCGGAATAC AACATTGTCG GCAATAATTT CTCTCCTTAT 
AAAGAAGACA TATTTGAAGA ATGTAAAAAA CTTTTGGGCC ATACACTTTA TCAGTTGTTT 
AACAACGTCG GTTGTCTAAC GAGTATTTGG TGGTTAAATA TAAAAGATAG GACATTACAA 
AATGATATTG AAAAGTTTGT CTCAGAGTTC ATCTCCCCAA TTTTGATCAA AAATGAATTT 
GACGATATCA ATTCTAAGAT GGACCGCCTG ACTTCTAATG ATGATGCTTT GACTATTAAA 
TTAAATAATA TTACTAATGA AGTGAAGGCA AGCTATTTGA TTGACGACCA AAAGTTGGAG 
ATTTCCTTCA AACTACCAAA AAACTACCCT TTAACAAATA TCCAAGTTAA TGGCGTCTCT 
AGAGTTGGTA TAAGTGAACA AAAATGGAAA CAATGGATAA TGTCTACACA ACATGTAATT 
ACAGGCATGA ATGGATCCGT TTTGGATTCG TTGGAGTTGT TTACTAAAAA TGTGCACTTA 
CAATTTTCTG GTTTCGAGGA ATGTGCCATA TGTTACTCTA TACTACATGC CGTGGATAGG 
AAACTACCTT CGAAGACATG TCCGACTTGT AAAAATAAGT TCCACGGAGC ATGTCTTTAC 
AAGTGGTTTC GATCATCGGG TAACAATACA TGTCCATTAT GCCGTAGTGA AATTCCATTT 
AGAAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013975.1
CDS	1..4689
Translation	MSFGGINTFQQYNTDLGLGHNGVRISLNYFDGLPDPSLLNSLYSNELKLIFKSLLKRDETTKEKALMDLSNLISDFNQNEYFFNDIFLLCWSQIYAKLIISDYKVIRLQSHQITIMLVKSLRKKISKFLKDFIPLILLGTCELDYSVSKPSLNELTECFNKDPAKINALWAVFQEQLLNLVKEIVVNENEDTISDERYSSKEESEFRYHRVIASAVLLLIKLFVHNKDVSERNSSSLKVILSDESIWKLLNLKNGQNTNAYETVLRLIDVLYTRGYMPSHKNIMKLAVKKLLKSLTHITSKNILKVCPVLPSILNLLATLDDYEDGTIWSYDKSSKEKVLKFLSVSRTSPSPGFFNAVFALYSSTKRHSFLDYYLEWLPFWQKSVQRLNEKGFSARNSAEVLNEFWTNFLKFAEDSSEERVKKMVESEIFNSLSCGKSLSEYTKLNQTLSGVFPPDKWEREIEDYFTSDEDIRKIKVSFEKNLFALLVTSPNNESAISRLFDFFVQLIETDPSNVFNKYDGVYDALNYFLDSDMIFLNGKIGKFINEIPTLVQESTYQNFAGIMAQYSNSKFFKMNTDAITSLEDFFIVALSFNLPKTIILATMNELDNDIYQQLMKSDSLELELYIEDFMKNYKFDDSGEIFKGNNKFLNQRTITTLYRSAVANGQVEQFCAVLSKLDETFFSTLLLNTDFLSCALYEVSEDTNEKLFKLSLQLAKGNSEIANKLAQVILQHAQVYFSPGAKEKYVTHAVELINGCNDTSQIFFPANAIEVFARYMPAIDYRSSLVSSLSTNTHLLLTDDKPINLKNMQKLIRYALFLDALLDALPERVNNHIVAFITVVSELVTDYNCLSEEPNDLYYDFGHTFFKHGKVNLNFSDIVGNVIQPANGGDAMLTFDIAESNSVYFFYYSRVLYKVLLNSIDTVSSTTLNGLLASVESFVTKTVRDQKSTDKDYLLCAILLLMFNRSNSKDEITKLRTLLASQLIGIREVELVDQEFKSLALLNNLLDIPQADKQFVPIAPQRLNMIFRSILKWLDSDLAYEPSFSTVRLLLLDFFTKLMRFEGVRDMGITAFELSERLLADSLSMCQIDDTLYLLELRSSCLNLYETLSQGVSKNGEEISEYGDEIQENLIELMFLNFNQERNNQVSTLFYQKLYKVISSMELKKLESQYKRIFEVVLNDKDIGSNINQSRLLTTLLGSLVVKTQQDIIIEYELRIQKQTGSDVDGSASDNDVNSKFKLPQKLLQKVTDEVPKEYLEYENKNSFIKYLWYWHLILMYFKDTSYNMRQIFIEQLKEAGLINRMFDFITDQIDLRDTEFWKQVDTKEISEYNIVGNNFSPYKEDIFEECKKLLGHTLYQLFNNVGCLTSIWWLNIKDRTLQNDIEKFVSEFISPILIKNEFDDINSKMDRLTSNDDALTIKLNNITNEVKASYLIDDQKLEISFKLPKNYPLTNIQVNGVSRVGISEQKWKQWIMSTQHVITGMNGSVLDSLELFTKNVHLQFSGFEECAICYSILHAVDRKLPSKTCPTCKNKFHGACLYKWFRSSGNNTCPLCRSEIPFRR

Target ORF information:

RefSeq Version	NM_001182755.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C ubiquitin-protein ligase RKR1 (RKR1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182755.1

ORF Insert Sequence:

ATGTCGTTTG GTGGAATCAA TACATTTCAA CAGTATAACA CAGATTTAGG GCTCGGCCAT 
AATGGTGTTA GAATATCCTT AAACTACTTT GATGGTTTGC CGGATCCAAG TCTTTTAAAC 
TCCTTGTACT CGAACGAGCT GAAACTCATT TTCAAATCTC TTCTGAAAAG AGATGAAACT 
ACAAAAGAAA AAGCTTTAAT GGATCTTTCC AATTTGATCA GCGACTTTAA CCAGAATGAG 
TACTTTTTCA ATGACATTTT TCTTCTTTGT TGGTCGCAGA TATATGCTAA ACTGATAATT 
AGTGATTACA AGGTCATCAG ACTGCAATCA CATCAAATTA CTATAATGCT TGTCAAAAGC 
CTTCGGAAGA AAATATCGAA GTTTTTAAAG GATTTTATAC CTTTGATACT TCTCGGTACA 
TGCGAACTCG ATTATAGTGT TTCCAAACCT TCTCTTAATG AATTAACAGA GTGTTTCAAC 
AAAGACCCAG CAAAAATTAA CGCCTTATGG GCTGTTTTTC AAGAACAACT ATTGAATTTA 
GTAAAGGAGA TTGTTGTAAA CGAGAATGAA GATACCATTT CTGATGAACG TTACTCCAGT 
AAAGAAGAAT CTGAGTTTAG ATACCACAGA GTTATAGCAA GTGCCGTATT GCTGCTGATT 
AAGCTTTTTG TTCATAACAA AGACGTCTCA GAACGTAATT CTTCCAGTCT TAAGGTGATT 
CTTTCTGATG AGTCAATCTG GAAACTACTT AACTTAAAAA ATGGGCAAAA TACAAATGCC 
TACGAAACAG TTCTTCGATT GATTGATGTT CTTTATACAA GAGGTTACAT GCCTTCCCAC 
AAAAATATAA TGAAGTTAGC GGTGAAAAAA CTATTGAAAT CGTTGACTCA TATTACGTCA 
AAGAATATTC TAAAAGTATG CCCTGTTCTC CCATCAATAC TGAACCTTTT AGCTACCTTG 
GATGACTATG AAGATGGGAC AATATGGTCA TACGATAAAT CTTCTAAAGA AAAAGTACTC 
AAATTTTTAT CGGTTTCCCG TACTTCACCA TCGCCAGGAT TTTTCAATGC TGTGTTTGCA 
TTGTACAGCT CGACTAAAAG ACACAGTTTT CTTGATTATT ATCTTGAATG GCTTCCATTT 
TGGCAAAAAT CGGTGCAAAG ACTGAATGAA AAGGGCTTTT CTGCGAGAAA CAGCGCTGAG 
GTTCTTAATG AGTTTTGGAC AAATTTTTTG AAGTTCGCTG AGGATTCATC AGAAGAAAGA 
GTGAAAAAAA TGGTGGAAAG TGAGATTTTT AACTCTCTGT CTTGCGGGAA ATCTTTATCT 
GAGTACACAA AACTGAACCA GACTCTTAGT GGCGTCTTTC CACCCGATAA ATGGGAGAGA 
GAGATTGAAG ATTACTTCAC TTCAGACGAA GACATAAGAA AAATAAAGGT GAGCTTTGAG 
AAGAATCTTT TTGCTTTATT GGTTACTTCT CCTAATAACG AATCCGCAAT TAGTCGTTTA 
TTCGATTTTT TTGTGCAATT AATCGAAACA GATCCCTCAA ATGTTTTCAA CAAATACGAC 
GGTGTATATG ATGCTCTCAA TTACTTTTTA GATTCAGATA TGATTTTCTT GAATGGGAAG 
ATAGGGAAAT TTATAAACGA AATTCCAACG TTGGTCCAAG AATCAACATA TCAAAACTTT 
GCTGGTATCA TGGCACAGTA CTCAAACTCC AAATTTTTCA AAATGAATAC TGACGCGATT 
ACTTCGTTGG AAGACTTTTT TATCGTTGCG CTTTCTTTTA ACTTACCAAA GACAATAATT 
TTAGCCACAA TGAATGAGTT GGACAATGAT ATTTATCAAC AATTAATGAA ATCAGATTCC 
CTAGAATTAG AATTGTACAT TGAAGACTTT ATGAAAAATT ATAAGTTTGA CGACAGTGGG 
GAAATATTTA AAGGTAATAA TAAATTTTTA AATCAAAGGA CTATCACCAC ACTTTACCGA 
AGCGCAGTTG CCAATGGCCA AGTTGAACAA TTTTGTGCTG TTTTGTCTAA ATTAGATGAA 
ACTTTTTTTT CAACGTTATT GCTGAATACC GATTTTCTTT CTTGTGCATT GTATGAAGTT 
TCAGAAGACA CTAATGAGAA ACTATTCAAG TTGTCATTAC AGTTGGCTAA AGGGAATTCT 
GAAATCGCTA ATAAGTTGGC CCAAGTGATT TTGCAACATG CTCAGGTTTA TTTCAGCCCC 
GGGGCAAAAG AAAAATATGT CACACACGCC GTGGAGCTAA TTAATGGCTG CAACGATACT 
TCTCAGATTT TTTTTCCCGC TAACGCAATC GAAGTTTTTG CCAGATATAT GCCAGCAATT 
GATTATAGGT CATCCTTAGT GAGCAGCTTA AGTACTAACA CGCATTTGCT CCTTACAGAT 
GATAAGCCTA TCAATTTAAA AAATATGCAA AAGCTCATCA GGTATGCTCT ATTCTTAGAT 
GCCCTACTTG ATGCTCTTCC AGAGCGCGTT AATAACCATA TTGTTGCATT TATAACTGTA 
GTTTCTGAGC TCGTCACTGA TTATAATTGC CTTTCAGAGG AACCAAATGA TCTTTACTAT 
GATTTTGGGC ATACCTTTTT CAAACATGGC AAAGTAAATT TGAACTTTTC TGATATTGTT 
GGAAACGTTA TTCAGCCTGC TAATGGTGGT GATGCTATGC TTACTTTTGA TATTGCTGAA 
AGTAATTCTG TCTACTTTTT TTATTATTCA AGAGTATTAT ATAAGGTTCT ATTGAACAGC 
ATTGATACTG TTTCATCCAC CACATTAAAT GGGCTATTAG CATCGGTTGA AAGTTTTGTC 
ACGAAAACTG TGAGGGATCA AAAATCTACT GATAAAGATT ATCTCCTATG TGCTATATTA 
CTATTGATGT TTAACAGGTC CAACTCAAAA GATGAGATAA CGAAATTAAG GACATTGCTG 
GCATCTCAAT TGATTGGGAT TAGAGAAGTA GAACTTGTCG ATCAAGAATT CAAGTCGTTA 
GCTCTTTTAA ATAATCTGCT GGATATCCCA CAGGCAGATA AACAGTTTGT TCCTATAGCA 
CCGCAGAGAC TGAACATGAT ATTCCGTTCA ATACTAAAAT GGTTGGATAG TGATCTTGCC 
TACGAGCCTA GCTTTAGCAC CGTTAGATTG TTACTACTAG ATTTTTTCAC CAAACTGATG 
AGGTTTGAAG GAGTCAGAGA TATGGGCATA ACAGCTTTTG AACTTTCGGA AAGGTTGCTG 
GCAGACTCTC TATCGATGTG TCAAATTGAT GACACTTTAT ATTTGTTAGA ACTTCGCTCT 
TCCTGTTTGA ACTTATATGA AACATTGTCG CAAGGCGTTT CCAAAAATGG CGAGGAAATC 
TCAGAATATG GTGATGAAAT TCAAGAAAAT TTGATTGAAC TGATGTTTCT CAACTTTAAT 
CAAGAAAGAA ATAACCAAGT ATCCACACTC TTCTACCAGA AATTGTACAA GGTCATTTCC 
TCAATGGAAC TCAAAAAGTT AGAATCTCAA TACAAAAGAA TATTCGAGGT TGTATTGAAT 
GATAAGGATA TCGGTAGTAA TATTAATCAA TCAAGATTAT TAACTACTTT GTTGGGTTCT 
TTGGTGGTAA AAACACAGCA GGATATTATA ATTGAGTATG AACTGAGGAT TCAAAAACAA 
ACTGGCTCAG ACGTAGATGG TAGTGCATCT GATAATGATG TAAACTCAAA ATTTAAACTA 
CCTCAAAAAT TATTACAAAA AGTTACTGAT GAGGTGCCAA AAGAATACCT TGAATACGAG 
AATAAAAATT CATTTATTAA ATATCTATGG TATTGGCACT TGATACTGAT GTACTTCAAA 
GATACGTCTT ATAATATGAG ACAAATATTT ATTGAACAAT TAAAGGAAGC TGGTTTAATC 
AATAGAATGT TTGATTTCAT AACAGATCAA ATAGATTTAA GAGATACTGA GTTTTGGAAA 
CAGGTCGACA CCAAGGAAAT TTCGGAATAC AACATTGTCG GCAATAATTT CTCTCCTTAT 
AAAGAAGACA TATTTGAAGA ATGTAAAAAA CTTTTGGGCC ATACACTTTA TCAGTTGTTT 
AACAACGTCG GTTGTCTAAC GAGTATTTGG TGGTTAAATA TAAAAGATAG GACATTACAA 
AATGATATTG AAAAGTTTGT CTCAGAGTTC ATCTCCCCAA TTTTGATCAA AAATGAATTT 
GACGATATCA ATTCTAAGAT GGACCGCCTG ACTTCTAATG ATGATGCTTT GACTATTAAA 
TTAAATAATA TTACTAATGA AGTGAAGGCA AGCTATTTGA TTGACGACCA AAAGTTGGAG 
ATTTCCTTCA AACTACCAAA AAACTACCCT TTAACAAATA TCCAAGTTAA TGGCGTCTCT 
AGAGTTGGTA TAAGTGAACA AAAATGGAAA CAATGGATAA TGTCTACACA ACATGTAATT 
ACAGGCATGA ATGGATCCGT TTTGGATTCG TTGGAGTTGT TTACTAAAAA TGTGCACTTA 
CAATTTTCTG GTTTCGAGGA ATGTGCCATA TGTTACTCTA TACTACATGC CGTGGATAGG 
AAACTACCTT CGAAGACATG TCCGACTTGT AAAAATAAGT TCCACGGAGC ATGTCTTTAC 
AAGTGGTTTC GATCATCGGG TAACAATACA TGTCCATTAT GCCGTAGTGA AATTCCATTT 
AGAAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Nature387(6632 Suppl)90-3(1997 May)
Bowman S,Churcher C,Badcock K,Brown D,Chillingworth T,Connor R,Dedman K,Devlin K,Gentles S,Hamlin N,Hunt S,Jagels K,Lye G,Moule S,Odell C,Pearson D,Rajandream M,Rice P,Skelton J,Walsh S,Whitehead S,Barrell B

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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RKR1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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