ESC1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	ESC1
Entrez Gene ID	855259
Full Name	Esc1p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182726.1	NP_013946.1	Esc1p

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Bowman S, Churcher C, Badcock K, Brown D, Chillingworth T, Connor R, Dedman K, Devlin K, Gentles S, Hamlin N, Hunt S, Jagels K, Lye G, Moule S, Odell C, Pearson D, Rajandream M, Rice P, Skelton J, Walsh S, Whitehead S, Barrell B
Nature387(6632 Suppl)90-3(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that Esc1 and Ulp1 help retain unspliced pre-mRNAs in the nucleus, and are essential for proper nuclear basket function, which includes mRNA surveillance and regulation of SUMO protein dynamics.
Title: A nuclear envelope protein linking nuclear pore basket assembly, SUMO protease regulation, and mRNA surveillance.

Ku- and Esc1-dependent pathways mediate natural telomere anchoring in vivo
Title: Separation of silencing from perinuclear anchoring functions in yeast Ku80, Sir4 and Esc1 proteins.

The following ESC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ESC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04214	NM_001182726.1 Latest version!	Saccharomyces cerevisiae S288C Esc1p (ESC1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ESC1 ( NM_001182726.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013946.1 Saccharomyces cerevisiae S288C Esc1p (ESC1), partial mRNA.

Price & Availability↑

CloneID	OSi04214
Clone ID Related Accession (Same CDS sequence)	NM_001182726.1
Accession Version	NM_001182726.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4977bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Esc1p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001145). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCAAAAA AAAAAGAAAC CTTTACTCCA AGGGCAAATA AACTTAAACT GACGACCCCA 
AGAAGAAAAC TGAAAATACT GTCATCTCTC CTTGACGCGG ACGAGGATTC CAAAATGAAA 
GATCAACATG GGTACTCAAG GGTCCACAAT GATAAATACA GAGTAGCAAA GCCTACTCAG 
CACTCGACTT TACATGAATC TATTTCCTCC AGGCGAAGCA GTCATATTCA TAATAAATCC 
CTTCATGAAG ATAGTGCGAG GGCATTAAGC TGGGTAGATT CTCTAATTAA TAGGGGGAAA 
AGTATACTTA CTACACTAGA AAAGGAAGAT GCCTTGTTTG AGCGTAGTCT TGAGGAGGAA 
AGACAACGTT TTCAGCTTCA TGACTCTTTA ATGAACAAGT ACACAGGAAA CAGTAAATCT 
CATCAGCGAC TAATCGATCT CAGGAAGAGC CAATACGGAA CGGATACTTC TTTCCAAAAT 
AATGATGAAA TTCCCTTAGA TAGTTTCATA AGTTCTCCCT TACCGGACGC TGAGGACGAG 
TCGTCCTCAA ATATCGACTC CGATAAGGAT GAAGATCTGG AAGGTAAACA ATCTTTAATC 
AAAGATTTCG ATCTTGAAAA TGATGAATAC GAGCTCTCTG AAGAAGAAAA GAATAGTGAC 
GGCCAAAGTA GTCCTTCAAT AATGATATTA TCAGATGAAG AATATGCAGA GGAAGGAGCA 
CTACAAGATG TATCGAATGA TGAATATGCC GAAGAGGAGG GGCAAGTTGA GAGAAAGAAT 
ATAGGGCAAG AACAGGCAAA TGTAGAGAAT GCTACACAAA TTAGTTCTTC TGATTCCAGT 
GAAGGACAGA ATTATTCAGA GGGAGTAGAA ATGGAATTAG AAGACGACAT TGATGTGGAA 
AGCGATGCTG AAAAAGATGA AAGTCAGGGT GCAGAAGGAA CAGAACATTC GGTAGATTTT 
TCAAAATACA TGCAGCCTAG AACAGATAAT ACTAAGATCC CCGTTATCGA AAAATATGAA 
TCTGATGAGC ACAAAGTCCA TCAAAGATAT TCAGAAGACG GTGCATTTGA TTTCGGTTCA 
GTAAATATTT CAGTTGACGA TGAGTCCGAG GATGAAGAAA GTCAAGCTGA AAGTTACTCA 
GCAAATGCTG AAAATGTGTA CCACCATAAT GAACATGAAC TGGATGATAA GGAACTAATA 
GAAGATATAG AATCTTCAGA TAGTGAATCC CAAAGTGCAC AAGAAAGCGA ACAAGGAAGT 
GAAGATGATT TTGAATATAA AATGAAAAAC GAAAAAAGCA CGTCAGAAGA AACTGAGAAT 
ACCAGCGAAA GCAGAGACCA AGGTTTCGCT AAAGATGCTT ATACTAAGAA CAAAGTGGAA 
CAACAGGAAA ATGACGAAGA ACCTGAAAAA GATGACATTA TCAGAAGCTC TCTAGACAAA 
AATTTTCACG GAAACAACAA CAAAAGTGAA TACTCGGAAA ATGTTTTGGA GAATGAAACT 
GATCCTGCTA TAGTAGAAAG AGAAAATCAA ATTAACGATG TTGAAGGTTA TGACGTAACA 
GGAAAAAGTG TCGAAAGTGA TTTGCACGAG CATTCCCCTG ATAATTTGTA TGATCTCGCC 
GCAAGGGCTA TGCTTCAATT CCAACAAAGT AGAAATTCGA ACTGCCCTCA AAAAGAGGAG 
CAGGTATCCG AATCTTATTT AGGGCACTCA AATGGAAGCA ACTTATCGGG CAGGTCTTTA 
GACGAATCCG AAGAGCAAAT ACCCCTAAAG GACTTCACAG GTGAAAACAA TAATAATTTA 
AAAACAGACC GAGGTGATCT TTCATCTTCT GTTGAAATAG AGGTTGAAAA AGTTTCTGAA 
AAGAAACTGG ACGGTTCTAC AGAAAAAGAA CTGGTACCAC TATCCACAGA TACTACGATT 
AACAATTCAT CATTAGGAAA TGAAGACAGT ATCTATTATT CACTCGATGA CGCTGATGCT 
ATATCAGAGA ATCTTACCGA TGTTCCTCTT ATGGAAATCA AAACAACTCC GAAATATGAA 
GTAGTAATTT CTGAATCTGT GTATAGTTCT ACTTCTTACG AAGATAACAC AGTTGCCATG 
CCACCGCAAG TGGAGTACAC TTCACCATTT ATGAATGACC CATTCAATTC GTTGAACGAT 
GATTACGAAA AGAAGCATGA CCTACTGAAA TCAACGTTAG CAGCCCTTGC CCCAGCTTTC 
ACCAAAAAGG ATGCAGAATT TGTGGAAGCT GGAGTGACGA AATCATGCTT GACATCTACA 
TCTGGGCACA CAAACATTTT TCACACAAGT AAGGAAACTA AACAGGTGAG CGATTTGGAT 
GAATCTACGG AAAACGTTAC TTTTGAAAAT GAAAATACAG GTGATGAAAA TAAAAATCAA 
AGTAAAAATT TTCCAGGCGT GGCTAACAGT ACTGACAAGT CAACTGAAGA TAACACGGAT 
GAAAAGTACT TTTCAGCGAT TAATTATACC AACGTTACAG GAGACTCTTC CTGCGAGGAC 
ATAATTGAAA CGGCTTCAAA TGTAGAAGAA AATTTAAGAT ACTGTGAAAA GGATATGAAC 
GAAGCTGAGA TGTCTTCTGG CGATGAATGC GTAAAACAGA ATGATGATGG TAGTAAAACT 
CAAATTTCGT TTTCAACAGA TTCGCCAGAC AATTTTCAAG AAAGCAACGA CAACACTGAA 
TTCTCTTCTA CTAAATATAA AGTAAGAAAT AGTGATCTTG AAGACGACGA ATCACTCAAG 
AAGGAACTTA CAAAAGCTGA AGTAGTTGAT AAGCTTGATG AAGAGGAAAG TGAGGATTCA 
TATGAACAAG ATTATGCGGA TCCAGAACCA GGCAATGATG AAGGCAGTAA TGAAAATATA 
GTTAAAGGTA CAAAAAAGGA TACTCTCGGA ATAGTAGAAC CAGAAAATGA AAAAGTTAAT 
AAGGTGCACG AAGAAGAAAC ACTTTTCGAA GCCAATGTCA GTTCATCCGT AAATGTTCAG 
AACAAAGACA TGCACACCGA CGTCATCAAT CAAGAGGCGC AAGCGAATTA CGAAGCTGGT 
GAAAGAAAAT ACTATATCCA GAATACAGAT ACGGAGGAAG CACACATTTC CATTATCGAA 
AGAATAGATG AGAATGCGAT TGGAAATAAT ATGGAGATCC CAGAAAGGTC GTGCGTGGAA 
AAAACGCACA ACGAAGTGCT CTTTGAAAGA AGAGCAACTA CCATTGAAAA CACAAAAGCC 
CTGGAAAATA ATACCAATAT GCATGATCAG GTTAGCCAAG CTTGCTCTGA TTCAGACCGA 
GACCAAGACT CCACAGCTGA AAAAAATGTT GAGGGATCTG CAAAACATAA TTTGGACATT 
CGGGTGTCCA GTTCGGAAAT TGAGTCTGTG GAACCATTGA AGCCAGAATC TGATCGATCA 
AATATTTTTT CATCCCCAAT TCGTGTTATT GGAGCTGTAG TTAAGGGAGT GGGTAAGGTA 
GTCGACGTAG CTGAATCATT CGTGAAAAAA ATTGATGTTA TGGATTCTGA GAGTGATGAT 
AACGTTGATA TTGGTGATTA TAATCAAGAC ATTTTTAACA AGTCGAATTC TACCGATGCA 
TCTGTTAATA TGAAAAGTGT AAGTAGCAAA GAGAGAGACA GCGATGAAGA TGAAGCTGTC 
ATACTCGGAG GAGTTACCGC AGAAGCACAT AATGACAATG GTAATAATTC TCGTGTGATA 
AACATCGATC CCACGACAAA CGGTGCTTAT GAAGAGGACT CCGAAGTGTT TCGACAGCAA 
GTAAAGGACA AGGAAAATTT GCATAAGTCG GAAGAGCCGC TAGTTGAAGG TTTACAATCC 
GAGCAACATT TCGAAAAGAA AGACCACAGC GAGAATGAAG AAGAATTTGA TACCATATAT 
GGAGATATAA CATCAGCAAA TATTCACAGT AATGCCCCCG ATGATATTAA GAGACAACAA 
CTCCTAAAGA ATCTAAGTGA CCTAGAGAAT TATTCTCAGA GGCTCATTGA AGATTCCAGG 
AGAGGAAAAA ATCAAGAAGA ATCAGACGAA GTTAATACAA GCCGTGAACG GGATCTCACG 
TTTGAAAAGT CCGTTAATGA GAAATATGCT GGAGCAATTG AAGAAGACAC TTTTTCGGAG 
TTAGATATTA GCATTCAACA CCCCGAACAT GAAGAAGACC TGGACCTATC AAACAATCAG 
GAGAGGTCAA TAGAAGAACT AAATTCAGAG CCAGAAGAAG CTGAGTTGTA CGAATTGGAA 
ATAGAGGGCC CTACAGAGAC AGCAGCTTCT AGTAAAATGA ATGATGATGA AAGGCAACGC 
GGAAATATAC CGTCCACAGA TCTTCCATCT GATCCTCCGT CTGATAAGGA AGAAGTGACA 
GATTCTTATC CCTATTCAAA TTCGGAAAAT ATTACCGCTG AAAAGAGTGC GCCAACATCA 
CCGGAAGTGT ATGAAATATT CTCAGACACG CCAAATGAAG TCCCTATGGA AATTAATGAT 
GAAATTCCCG CCACCACGCT TGAGAAACAC GATAAAACCA ATGTAACATC CGTTTTGGAT 
GATAGATCAG AACATTTATC TAGCCACGAT GTTGATAATG AGCCACACGA TAATTCCATT 
AACATCAAAG TAAATGAAGG TGAGGAGCCC GAACATCAAG CAGTGGATAT ACCTGTTAAA 
GTAGAAGTTA AAGAAGAACA GGAGGAAATG CCTTCAAAAT CTGTACTTGA AGAACAGAAA 
CCAAGTATGG AACTAATCAA TGATAAAAGT TCTCCAGAAA ATAATAATGA TGAGGAAACA 
AACCGCGAAA AGGACAAAAC TAAAGCAAAA AAGAAAAGCC GTAAAAGAAA CTACAACAGC 
AGAAGACGGA AAAGAAAAAT CACAGAAGGC AGTTCTGCCG CATCAAATAC TAAAAGGCGT 
AGGGGGCACG AGCCAAAAAG CCGTGGACAG AATACGCATC CAAGTGTTGA CAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013946.1
CDS	1..4977
Translation	MSKKKETFTPRANKLKLTTPRRKLKILSSLLDADEDSKMKDQHGYSRVHNDKYRVAKPTQHSTLHESISSRRSSHIHNKSLHEDSARALSWVDSLINRGKSILTTLEKEDALFERSLEEERQRFQLHDSLMNKYTGNSKSHQRLIDLRKSQYGTDTSFQNNDEIPLDSFISSPLPDAEDESSSNIDSDKDEDLEGKQSLIKDFDLENDEYELSEEEKNSDGQSSPSIMILSDEEYAEEGALQDVSNDEYAEEEGQVERKNIGQEQANVENATQISSSDSSEGQNYSEGVEMELEDDIDVESDAEKDESQGAEGTEHSVDFSKYMQPRTDNTKIPVIEKYESDEHKVHQRYSEDGAFDFGSVNISVDDESEDEESQAESYSANAENVYHHNEHELDDKELIEDIESSDSESQSAQESEQGSEDDFEYKMKNEKSTSEETENTSESRDQGFAKDAYTKNKVEQQENDEEPEKDDIIRSSLDKNFHGNNNKSEYSENVLENETDPAIVERENQINDVEGYDVTGKSVESDLHEHSPDNLYDLAARAMLQFQQSRNSNCPQKEEQVSESYLGHSNGSNLSGRSLDESEEQIPLKDFTGENNNNLKTDRGDLSSSVEIEVEKVSEKKLDGSTEKELVPLSTDTTINNSSLGNEDSIYYSLDDADAISENLTDVPLMEIKTTPKYEVVISESVYSSTSYEDNTVAMPPQVEYTSPFMNDPFNSLNDDYEKKHDLLKSTLAALAPAFTKKDAEFVEAGVTKSCLTSTSGHTNIFHTSKETKQVSDLDESTENVTFENENTGDENKNQSKNFPGVANSTDKSTEDNTDEKYFSAINYTNVTGDSSCEDIIETASNVEENLRYCEKDMNEAEMSSGDECVKQNDDGSKTQISFSTDSPDNFQESNDNTEFSSTKYKVRNSDLEDDESLKKELTKAEVVDKLDEEESEDSYEQDYADPEPGNDEGSNENIVKGTKKDTLGIVEPENEKVNKVHEEETLFEANVSSSVNVQNKDMHTDVINQEAQANYEAGERKYYIQNTDTEEAHISIIERIDENAIGNNMEIPERSCVEKTHNEVLFERRATTIENTKALENNTNMHDQVSQACSDSDRDQDSTAEKNVEGSAKHNLDIRVSSSEIESVEPLKPESDRSNIFSSPIRVIGAVVKGVGKVVDVAESFVKKIDVMDSESDDNVDIGDYNQDIFNKSNSTDASVNMKSVSSKERDSDEDEAVILGGVTAEAHNDNGNNSRVINIDPTTNGAYEEDSEVFRQQVKDKENLHKSEEPLVEGLQSEQHFEKKDHSENEEEFDTIYGDITSANIHSNAPDDIKRQQLLKNLSDLENYSQRLIEDSRRGKNQEESDEVNTSRERDLTFEKSVNEKYAGAIEEDTFSELDISIQHPEHEEDLDLSNNQERSIEELNSEPEEAELYELEIEGPTETAASSKMNDDERQRGNIPSTDLPSDPPSDKEEVTDSYPYSNSENITAEKSAPTSPEVYEIFSDTPNEVPMEINDEIPATTLEKHDKTNVTSVLDDRSEHLSSHDVDNEPHDNSINIKVNEGEEPEHQAVDIPVKVEVKEEQEEMPSKSVLEEQKPSMELINDKSSPENNNDEETNREKDKTKAKKKSRKRNYNSRRRKRKITEGSSAASNTKRRRGHEPKSRGQNTHPSVDK

Target ORF information:

RefSeq Version	NM_001182726.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Esc1p (ESC1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182726.1

ORF Insert Sequence:

ATGTCAAAAA AAAAAGAAAC CTTTACTCCA AGGGCAAATA AACTTAAACT GACGACCCCA 
AGAAGAAAAC TGAAAATACT GTCATCTCTC CTTGACGCGG ACGAGGATTC CAAAATGAAA 
GATCAACATG GGTACTCAAG GGTCCACAAT GATAAATACA GAGTAGCAAA GCCTACTCAG 
CACTCGACTT TACATGAATC TATTTCCTCC AGGCGAAGCA GTCATATTCA TAATAAATCC 
CTTCATGAAG ATAGTGCGAG GGCATTAAGC TGGGTAGATT CTCTAATTAA TAGGGGGAAA 
AGTATACTTA CTACACTAGA AAAGGAAGAT GCCTTGTTTG AGCGTAGTCT TGAGGAGGAA 
AGACAACGTT TTCAGCTTCA TGACTCTTTA ATGAACAAGT ACACAGGAAA CAGTAAATCT 
CATCAGCGAC TAATCGATCT CAGGAAGAGC CAATACGGAA CGGATACTTC TTTCCAAAAT 
AATGATGAAA TTCCCTTAGA TAGTTTCATA AGTTCTCCCT TACCGGACGC TGAGGACGAG 
TCGTCCTCAA ATATCGACTC CGATAAGGAT GAAGATCTGG AAGGTAAACA ATCTTTAATC 
AAAGATTTCG ATCTTGAAAA TGATGAATAC GAGCTCTCTG AAGAAGAAAA GAATAGTGAC 
GGCCAAAGTA GTCCTTCAAT AATGATATTA TCAGATGAAG AATATGCAGA GGAAGGAGCA 
CTACAAGATG TATCGAATGA TGAATATGCC GAAGAGGAGG GGCAAGTTGA GAGAAAGAAT 
ATAGGGCAAG AACAGGCAAA TGTAGAGAAT GCTACACAAA TTAGTTCTTC TGATTCCAGT 
GAAGGACAGA ATTATTCAGA GGGAGTAGAA ATGGAATTAG AAGACGACAT TGATGTGGAA 
AGCGATGCTG AAAAAGATGA AAGTCAGGGT GCAGAAGGAA CAGAACATTC GGTAGATTTT 
TCAAAATACA TGCAGCCTAG AACAGATAAT ACTAAGATCC CCGTTATCGA AAAATATGAA 
TCTGATGAGC ACAAAGTCCA TCAAAGATAT TCAGAAGACG GTGCATTTGA TTTCGGTTCA 
GTAAATATTT CAGTTGACGA TGAGTCCGAG GATGAAGAAA GTCAAGCTGA AAGTTACTCA 
GCAAATGCTG AAAATGTGTA CCACCATAAT GAACATGAAC TGGATGATAA GGAACTAATA 
GAAGATATAG AATCTTCAGA TAGTGAATCC CAAAGTGCAC AAGAAAGCGA ACAAGGAAGT 
GAAGATGATT TTGAATATAA AATGAAAAAC GAAAAAAGCA CGTCAGAAGA AACTGAGAAT 
ACCAGCGAAA GCAGAGACCA AGGTTTCGCT AAAGATGCTT ATACTAAGAA CAAAGTGGAA 
CAACAGGAAA ATGACGAAGA ACCTGAAAAA GATGACATTA TCAGAAGCTC TCTAGACAAA 
AATTTTCACG GAAACAACAA CAAAAGTGAA TACTCGGAAA ATGTTTTGGA GAATGAAACT 
GATCCTGCTA TAGTAGAAAG AGAAAATCAA ATTAACGATG TTGAAGGTTA TGACGTAACA 
GGAAAAAGTG TCGAAAGTGA TTTGCACGAG CATTCCCCTG ATAATTTGTA TGATCTCGCC 
GCAAGGGCTA TGCTTCAATT CCAACAAAGT AGAAATTCGA ACTGCCCTCA AAAAGAGGAG 
CAGGTATCCG AATCTTATTT AGGGCACTCA AATGGAAGCA ACTTATCGGG CAGGTCTTTA 
GACGAATCCG AAGAGCAAAT ACCCCTAAAG GACTTCACAG GTGAAAACAA TAATAATTTA 
AAAACAGACC GAGGTGATCT TTCATCTTCT GTTGAAATAG AGGTTGAAAA AGTTTCTGAA 
AAGAAACTGG ACGGTTCTAC AGAAAAAGAA CTGGTACCAC TATCCACAGA TACTACGATT 
AACAATTCAT CATTAGGAAA TGAAGACAGT ATCTATTATT CACTCGATGA CGCTGATGCT 
ATATCAGAGA ATCTTACCGA TGTTCCTCTT ATGGAAATCA AAACAACTCC GAAATATGAA 
GTAGTAATTT CTGAATCTGT GTATAGTTCT ACTTCTTACG AAGATAACAC AGTTGCCATG 
CCACCGCAAG TGGAGTACAC TTCACCATTT ATGAATGACC CATTCAATTC GTTGAACGAT 
GATTACGAAA AGAAGCATGA CCTACTGAAA TCAACGTTAG CAGCCCTTGC CCCAGCTTTC 
ACCAAAAAGG ATGCAGAATT TGTGGAAGCT GGAGTGACGA AATCATGCTT GACATCTACA 
TCTGGGCACA CAAACATTTT TCACACAAGT AAGGAAACTA AACAGGTGAG CGATTTGGAT 
GAATCTACGG AAAACGTTAC TTTTGAAAAT GAAAATACAG GTGATGAAAA TAAAAATCAA 
AGTAAAAATT TTCCAGGCGT GGCTAACAGT ACTGACAAGT CAACTGAAGA TAACACGGAT 
GAAAAGTACT TTTCAGCGAT TAATTATACC AACGTTACAG GAGACTCTTC CTGCGAGGAC 
ATAATTGAAA CGGCTTCAAA TGTAGAAGAA AATTTAAGAT ACTGTGAAAA GGATATGAAC 
GAAGCTGAGA TGTCTTCTGG CGATGAATGC GTAAAACAGA ATGATGATGG TAGTAAAACT 
CAAATTTCGT TTTCAACAGA TTCGCCAGAC AATTTTCAAG AAAGCAACGA CAACACTGAA 
TTCTCTTCTA CTAAATATAA AGTAAGAAAT AGTGATCTTG AAGACGACGA ATCACTCAAG 
AAGGAACTTA CAAAAGCTGA AGTAGTTGAT AAGCTTGATG AAGAGGAAAG TGAGGATTCA 
TATGAACAAG ATTATGCGGA TCCAGAACCA GGCAATGATG AAGGCAGTAA TGAAAATATA 
GTTAAAGGTA CAAAAAAGGA TACTCTCGGA ATAGTAGAAC CAGAAAATGA AAAAGTTAAT 
AAGGTGCACG AAGAAGAAAC ACTTTTCGAA GCCAATGTCA GTTCATCCGT AAATGTTCAG 
AACAAAGACA TGCACACCGA CGTCATCAAT CAAGAGGCGC AAGCGAATTA CGAAGCTGGT 
GAAAGAAAAT ACTATATCCA GAATACAGAT ACGGAGGAAG CACACATTTC CATTATCGAA 
AGAATAGATG AGAATGCGAT TGGAAATAAT ATGGAGATCC CAGAAAGGTC GTGCGTGGAA 
AAAACGCACA ACGAAGTGCT CTTTGAAAGA AGAGCAACTA CCATTGAAAA CACAAAAGCC 
CTGGAAAATA ATACCAATAT GCATGATCAG GTTAGCCAAG CTTGCTCTGA TTCAGACCGA 
GACCAAGACT CCACAGCTGA AAAAAATGTT GAGGGATCTG CAAAACATAA TTTGGACATT 
CGGGTGTCCA GTTCGGAAAT TGAGTCTGTG GAACCATTGA AGCCAGAATC TGATCGATCA 
AATATTTTTT CATCCCCAAT TCGTGTTATT GGAGCTGTAG TTAAGGGAGT GGGTAAGGTA 
GTCGACGTAG CTGAATCATT CGTGAAAAAA ATTGATGTTA TGGATTCTGA GAGTGATGAT 
AACGTTGATA TTGGTGATTA TAATCAAGAC ATTTTTAACA AGTCGAATTC TACCGATGCA 
TCTGTTAATA TGAAAAGTGT AAGTAGCAAA GAGAGAGACA GCGATGAAGA TGAAGCTGTC 
ATACTCGGAG GAGTTACCGC AGAAGCACAT AATGACAATG GTAATAATTC TCGTGTGATA 
AACATCGATC CCACGACAAA CGGTGCTTAT GAAGAGGACT CCGAAGTGTT TCGACAGCAA 
GTAAAGGACA AGGAAAATTT GCATAAGTCG GAAGAGCCGC TAGTTGAAGG TTTACAATCC 
GAGCAACATT TCGAAAAGAA AGACCACAGC GAGAATGAAG AAGAATTTGA TACCATATAT 
GGAGATATAA CATCAGCAAA TATTCACAGT AATGCCCCCG ATGATATTAA GAGACAACAA 
CTCCTAAAGA ATCTAAGTGA CCTAGAGAAT TATTCTCAGA GGCTCATTGA AGATTCCAGG 
AGAGGAAAAA ATCAAGAAGA ATCAGACGAA GTTAATACAA GCCGTGAACG GGATCTCACG 
TTTGAAAAGT CCGTTAATGA GAAATATGCT GGAGCAATTG AAGAAGACAC TTTTTCGGAG 
TTAGATATTA GCATTCAACA CCCCGAACAT GAAGAAGACC TGGACCTATC AAACAATCAG 
GAGAGGTCAA TAGAAGAACT AAATTCAGAG CCAGAAGAAG CTGAGTTGTA CGAATTGGAA 
ATAGAGGGCC CTACAGAGAC AGCAGCTTCT AGTAAAATGA ATGATGATGA AAGGCAACGC 
GGAAATATAC CGTCCACAGA TCTTCCATCT GATCCTCCGT CTGATAAGGA AGAAGTGACA 
GATTCTTATC CCTATTCAAA TTCGGAAAAT ATTACCGCTG AAAAGAGTGC GCCAACATCA 
CCGGAAGTGT ATGAAATATT CTCAGACACG CCAAATGAAG TCCCTATGGA AATTAATGAT 
GAAATTCCCG CCACCACGCT TGAGAAACAC GATAAAACCA ATGTAACATC CGTTTTGGAT 
GATAGATCAG AACATTTATC TAGCCACGAT GTTGATAATG AGCCACACGA TAATTCCATT 
AACATCAAAG TAAATGAAGG TGAGGAGCCC GAACATCAAG CAGTGGATAT ACCTGTTAAA 
GTAGAAGTTA AAGAAGAACA GGAGGAAATG CCTTCAAAAT CTGTACTTGA AGAACAGAAA 
CCAAGTATGG AACTAATCAA TGATAAAAGT TCTCCAGAAA ATAATAATGA TGAGGAAACA 
AACCGCGAAA AGGACAAAAC TAAAGCAAAA AAGAAAAGCC GTAAAAGAAA CTACAACAGC 
AGAAGACGGA AAAGAAAAAT CACAGAAGGC AGTTCTGCCG CATCAAATAC TAAAAGGCGT 
AGGGGGCACG AGCCAAAAAG CCGTGGACAG AATACGCATC CAAGTGTTGA CAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Nature387(6632 Suppl)90-3(1997 May)
Bowman S,Churcher C,Badcock K,Brown D,Chillingworth T,Connor R,Dedman K,Devlin K,Gentles S,Hamlin N,Hunt S,Jagels K,Lye G,Moule S,Odell C,Pearson D,Rajandream M,Rice P,Skelton J,Walsh S,Whitehead S,Barrell B

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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ESC1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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