HFA1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	HFA1
Entrez Gene ID	855247
Full Name	acetyl-CoA carboxylase HFA1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182714.1	NP_013934.1	acetyl-CoA carboxylase HFA1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Bowman S, Churcher C, Badcock K, Brown D, Chillingworth T, Connor R, Dedman K, Devlin K, Gentles S, Hamlin N, Hunt S, Jagels K, Lye G, Moule S, Odell C, Pearson D, Rajandream M, Rice P, Skelton J, Walsh S, Whitehead S, Barrell B
Nature387(6632 Suppl)90-3(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

expression and evolution of the non-canonically translated yeast mitochondrial acetyl-CoA carboxylase Hfa1p
Title: Expression and evolution of the non-canonically translated yeast mitochondrial acetyl-CoA carboxylase Hfa1p.

The following HFA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HFA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04202	NM_001182714.1 Latest version!	Saccharomyces cerevisiae S288C acetyl-CoA carboxylase HFA1 (HFA1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HFA1 ( NM_001182714.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013934.1 Saccharomyces cerevisiae S288C acetyl-CoA carboxylase HFA1 (HFA1), partial mRNA.

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CloneID	OSi04202
Clone ID Related Accession (Same CDS sequence)	NM_001182714.1
Accession Version	NM_001182714.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	acetyl-CoA carboxylase HFA1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001145). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAGATCTA TAAGAAAATG GGCGTACGAG ACGTTCAATG ATGAAAAAAT CATTCAATTC 
GTGGTAATGG CGACACCTGA TGATTTACAC GCAAATTCGG AGTATATTAG AATGGCAGAC 
CAATATGTGC AGGTACCAGG GGGTACCAAC AACAACAATT ACGCCAACAT AGACTTAATA 
CTGGACGTGG CAGAGCAAAC GGATGTGGAT GCGGTCTGGG CTGGATGGGG CCATGCTTCT 
GAAAATCCGT GTCTTCCTGA GCTGTTAGCT AGTTCACAAA GGAAAATACT ATTCATTGGT 
CCTCCTGGAC GCGCTATGAG ATCATTGGGT GACAAGATTT CTTCCACTAT TGTAGCACAA 
AGCGCTAAAA TCCCGTGTAT CCCTTGGTCT GGTTCACATA TAGACACTAT CCATATCGAT 
AACAAGACGA ACTTTGTATC TGTGCCGGAT GATGTATATG TAAGGGGATG TTGTTCCTCA 
CCTGAAGATG CTTTAGAAAA GGCTAAATTA ATAGGATTTC CTGTAATGAT TAAGGCATCC 
GAAGGTGGTG GAGGTAAGGG CATTAGGCGA GTAGATAATG AGGATGATTT TATTGCATTA 
TATCGCCAAG CAGTGAATGA GACACCTGGG TCGCCTATGT TTGTTATGAA AGTTGTCACT 
GATGCTCGTC ACTTAGAGGT ACAGTTATTA GCTGACCAAT ATGGCACTAA CATTACATTG 
TTTGGGAGAG ACTGTTCCAT ACAAAGGCGG CACCAAAAGA TTATAGAAGA GGCACCAGTG 
ACAATAACCA AGCCTGAAAC GTTTCAAAGG ATGGAACGCG CAGCAATTCG TCTAGGTGAA 
TTGGTAGGTT ATGTTTCTGC GGGCACTGTC GAATACTTAT ATTCACCAAA AGATGATAAA 
TTTTACTTTT TAGAACTGAA TCCGAGACTA CAAGTAGAGC ATCCAACGAC AGAAATGATA 
TCTGGCGTAA ACCTTCCTGC CACTCAACTG CAAATCGCCA TGGGCATTCC TATGCACATG 
ATAAGTGATA TCAGAAAACT TTATGGTTTA GATCCAACGG GAACTTCGTA TATTGATTTT 
AAAAATTTAA AGAGACCCTC GCCAAAAGGC CATTGTATTT CATGCAGGAT CACTTCAGAA 
GATCCTAATG AAGGTTTCAA GCCCTCCACT GGGAAAATAC ATGAGCTCAA TTTTCGTTCT 
TCTTCCAATG TTTGGGGTTA CTTCTCAGTA GGAAATAATG GTGCTATTCA CTCATTTTCA 
GATTCCCAAT TTGGGCACAT TTTTGCTGTA GGAAACGATA GGCAAGATGC AAAGCAAAAC 
ATGGTTTTAG CTCTAAAAGA TTTTTCCATC CGAGGAGAAT TCAAAACCCC CATAGAGTAC 
CTGATAGAGC TATTAGAAAC TCGGGACTTT GAGAGTAATA ACATATCGAC TGGTTGGTTA 
GATGATTTGA TTTTGAAAAA TTTATCTTCC GATAGCAAAC TAGATCCAAC GCTCGCTATT 
ATCTGTGGTG CCGCAATGAA AGCATACGTT TTCACAGAAA AGGTGAGGAA TAAGTATTTG 
GAATTATTGC GGAGGGGCCA AGTTCCACCT AAAGATTTTC TTAAAACGAA GTTTCCTGTT 
GACTTCATTT TCGATAATAA TAGATACTTG TTCAATGTTG CTCAATCATC TGAAGAACAA 
TTTATTCTTT CTATCAATAA GTCTCAATGT GAAGTTAATG TTCAAAAATT GTCCAGTGAC 
TGCTTGTTGA TCTCCGTTGA CGGTAAATGC CATACAGTTT ATTGGAAGGA CGATATCAGA 
GGTACAAGAC TTTCGATAGA CTCCAATACC ATATTTTTAG AAGCTGAACT CAATCCCACT 
CAAGTGATCT CTCCAACTCC GGGGAAATTG GTGAAATATT TGGTCCGAAG TGGTGATCAC 
GTTTTTGCTG GACAGCAATA TGCAGAAATA GAAATAATGA AAATGCAGAT GCCACTAGTA 
GCGAAAAGTG ATGGTGTAAT TGAGTTACTA AGACAGCCCG GTTCCATAAT TGAGGCTGGT 
GATGTCATCG CAAAATTGAC TTTGGATTCG CCGTCCAAAG CTAACGAATC GTCTTTATAC 
CGCGGAGAAT TACCTGTTTT AGGTCCACCG CTAATAGAGG GTAGCCGACC AAACCATAAG 
CTCAGAGTCT TAATAAATAG GTTAGAAAAT ATTCTCAATG GATATCATGA AAACTCTGGA 
ATAGAAACTA CTCTAAAAGA GTTGATAAAA ATATTGAGAG ATGGTAGGCT TCCTTATTCA 
GAATGGGATT CCCAAATTTC TACGGTACGC AATAGACTAC CAAGGCAATT GAATGAGGGG 
CTGGGAAATC TAGTCAAGAA ATCTGTTTCT TTTCCTGCAA AGGAACTGCA CAAATTAATG 
AAGCGCTACT TGGAAGAAAA TACAAATGAT CATGTAGTTT ATGTTGCCTT ACAGCCACTT 
CTTAAAATTA GTGAAAGGTA TAGCGAAGGT TTAGCTAATC ACGAATGTGA AATTTTTTTA 
AAGTTGATTA AAAAGTACTA TGCTGTTGAG AAAATTTTTG AAAATCATGA TATACATGAA 
GAAAGAAACT TACTAAATCT GCGGAGGAAA GACCTTACAA ACTTGAAAAA AATTTTGTGC 
ATAAGTTTAT CGCATGCTAA CGTAGTCGCA AAGAACAAGT TAGTAACTGC AATATTGCAT 
GAATACGAGC CATTGTGCCA GGATTCCTCT AAGATGTCTT TAAAATTCAG GGCTGTTATA 
CATGATTTGG CAAGTTTGGA ATCTAAGTGG GCTAAAGAGG TTGCTGTAAA GGCAAGATCA 
GTGCTACTCA GAGGGATTTT CCCTCCCATA AAGAAAAGAA AAGAGCATAT TAAAACTCTC 
CTGCAATTGC ACATAAAGGA TACTGGTGCC GAAAACATTC ACAGCAGGAA CATATATTCC 
TGTATGAGGG ATTTTGGTAA TTTAATACAT TCAAATCTGA TACAACTTCA GGATTTGTTC 
TTTTTTTTTG GCCATCAAGA TACGGCTCTT TCCAGTATAG CATCTGAAAT TTATGCAAGG 
TATGCCTACG GCAATTATCA ATTAAAAAGT ATTAAGATTC ATAAAGGAGC GCCTGATTTA 
CTAATGTCAT GGCAATTCAG CTCATTAAGA AATTATTTAG TCAATTCTGA TGGTGAGAGT 
GATGAGTTTA CAAAACTTTC TAAACCTCCC TCAACATCAG GTAAGAGCTC AGCAAATAGT 
TTTGGTCTTC TTGTCAACAT GCGTGCGCTT GAATCTCTGG AAAAGACATT AGACGAGGTA 
TACGAACAAA TTCATATTCC TGAGGAAAGA CTTTCCAGCG GAGAGAACTC TCTTATTGTT 
AATATTTTAT CTCCTATTCG TTACAGAAGT GAAAATGATC TAATTAAAAC TTTAAAAATT 
AAACTTCATG AAAATGAGAG AGGTCTATCC AAGCTCAAGG TTAATCGTAT TACATTTGCA 
TTTATCGCCG CGAATGCGCC CGCTGTTAAA TTTTACTCCT TTGATGGAAC TACGTACGAT 
GAAATCTCTC AAATAAGAAA TATGGATCCA TCCTATGAAG CACCGTTAGA GTTAGGAAAA 
ATGTCGAACT ATAAAATCAG ATCACTACCT ACATACGATA GTAGTATACG CATTTTTGAA 
GGTATTAGCA AATTTACGCC GCTAGATAAA AGGTTCTTTG TCAGGAAAAT CATAAATTCC 
TTCATGTATA ATGATCAAAA AACAACCGAA GAAAACTTGA AAGCGGAAAT CAATGCTCAA 
GTGGTTTATA TGTTAGAACA TCTAGGAGCA GTTGACATCT CAAATTCAGA CTTGAATCAT 
ATTTTTTTAA GTTTCAATAC AGTTCTTAAC ATACCAGTAC ATCGTCTCGA GGAAATTGTG 
AGTACAATTC TAAAGACTCA CGAAACCAGA TTGTTTCAAG AAAGAATCAC AGATGTAGAA 
ATTTGCATCT CTGTTGAGTG CCTAGAAACA AAGAAGCCAG CCCCGCTTAG ATTACTTATT 
TCTAATAAAT CTGGGTATGT GGTAAAAATT GAGACATATT ACGAAAAGAT AGGGAAAAAT 
GGGAATCTGA TTTTGGAACC GTGTAGTGAG CAGAGCCATT ATAGCCAGAA ATCTCTCTCT 
CTTCCTTACT CGGTCAAGGA TTGGCTACAA CCTAAAAGGT ACAAAGCTCA ATTCATGGGT 
ACAACATATG TGTACGATTT CCCAGGTCTG TTTCATCAAG CTGCAATCCA ACAGTGGAAA 
AGGTATTTTC CAAAACATAA GCTGAATGAC AGTTTTTTTA GTTGGGTTGA ATTGATAGAA 
CAAAACGGTA ATTTAATAAA AGTAAACAGG GAGCCAGGCC TTAATAATAT AGGGATGGTT 
GCTTTTGAGA TTATGGTTCA GACACCTGAA TATCCTGAAG GGCGTAACAT GATCGTGATT 
TCTAATGATA TTACCTACAA TATTGGATCT TTTGGACCGA GAGAAGATTT GTTTTTTGAT 
AGGGTCACAA ATTATGCAAG AGAGAGAGGG ATCCCGAGGA TATACTTGGC GGCGAATTCA 
GGAGCTAAAT TGGGTATAGC CGAAGAGCTG ATCCCTCTAT TTCGTGTAGC ATGGAATGAC 
CCCTCTGATC CAACAAAGGG TTTCCAGTAC TTATACTTAG CTCCAAAAGA CATGCAGCTA 
CTGAAAGATT CTGGGAAAGG AAATTCGGTT GTTGTTGAAC ACAAGATGGT ATACGGTGAA 
GAGAGATATA TTATTAAAGC AATAGTCGGA TTCGAAGAGG GTTTAGGTGT TGAATGTTTA 
CAGGGCTCGG GTTTAATTGC TGGTGCCACT TCGAAAGCGT ATAGAGACAT TTTCACTATT 
ACTGCTGTTA CTTGTCGGTC CGTTGGTATA GGTTCCTATC TGGTCAGACT AGGACAACGT 
ACTATTCAGG TGGAGGATAA GCCTATCATA CTGACGGGTG CATCGGCGAT TAATAAAGTT 
TTGGGTACCG ATATCTATAC ATCTAACCTA CAAATTGGCG GAACCCAAAT CATGTATAAA 
AACGGAATAG CGCATTTAAC AGCCAGTAAT GATATGAAAG CCATCGAAAA AATAATGACA 
TGGTTATCAT ATGTCCCGGC GAAAAGAGAT ATGAGTCCTC CACTTCTTGA AACTATGGAT 
AGATGGGATA GGGATGTAGA CTTCAAACCT GCCAAGCAAG TGCCATATGA GGCAAGGTGG 
TTGATAGAGG GTAAATGGGA CTCAAATAAC AACTTCCAGT CAGGCCTATT TGATAAGGAT 
TCGTTTTTTG AGACATTATC TGGATGGGCC AAAGGTGTAA TAGTTGGAAG AGCACGTCTT 
GGAGGTATTC CCGTGGGTGT TATTGCGGTA GAAACTAAGA CTATCGAAGA AATAATCCCC 
GCTGACCCAG CTAATCTGGA TTCTTCAGAG TTTTCCGTTA AAGAAGCAGG ACAGGTGTGG 
TATCCAAATT CCGCGTTCAA AACAGCTCAA ACTATAAATG ATTTTAACTA TGGTGAGCAA 
TTACCATTGA TTATCTTAGC CAATTGGAGG GGATTTTCTG GCGGTCAAAG GGATATGTAC 
AATGAAGTAC TAAAGTACGG GTCTTTTATT GTTGACGCTC TGGTTGACTA CAAACAACCC 
ATACTGATAT ACATTCCGCC CTTTGGTGAA TTAAGGGGCG GATCATGGGT TGTTATAGAT 
CCAACTATTA ATCCTGAACA AATGGAAATG TATGCCGATG TTGAATCTAG GGGAGGTGTG 
TTAGAACCTG ACGGAGTAGT TAGCATAAAA TACCGTAAGG AGAAAATGAT AGAGACGATG 
ATTCGATTAG ACTCCACATA TGGACATTTG AGAAGAACGT TGACAGAAAA AAAGTTATCT 
TTGGAAAAAC AAAATGATCT TACGAAGAGA TTGAAAATAA GAGAGAGACA GTTGATACCA 
ATTTACAATC AAATCAGCAT ACAGTTTGCA GATTTACATG ATAGATCGAC TAGGATGCTA 
GTTAAAGGAG TAATCCGAAA TGAGTTGGAA TGGAAAAAGT CACGCAGATT TTTATATTGG 
AGACTGAGAA GGAGGTTGAA CGAGGGACAA GTGATCAAAA GACTGCAAAA AAAAACATGT 
GATAACAAAA CGAAAATGAA GTACGACGAC CTGTTGAAAA TAGTTCAGTC ATGGTATAAC 
GATCTGGATG TTAATGATGA CAGAGCAGTA GTGGAGTTCA TAGAAAGAAA TTCGAAAAAA 
ATTGACAAGA ACATTGAAGA GTTTGAGATC TCGCTGTTGA TCGATGAGCT TAAGAAAAAA 
TTTGAAGACA GAAGGGGAAA CATTGTCCTT GAAGAGCTAA CTAGGTTGGT GGACAGTAAG 
CGAAAGAGAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013934.1
CDS	1..6372
Translation	MRSIRKWAYETFNDEKIIQFVVMATPDDLHANSEYIRMADQYVQVPGGTNNNNYANIDLILDVAEQTDVDAVWAGWGHASENPCLPELLASSQRKILFIGPPGRAMRSLGDKISSTIVAQSAKIPCIPWSGSHIDTIHIDNKTNFVSVPDDVYVRGCCSSPEDALEKAKLIGFPVMIKASEGGGGKGIRRVDNEDDFIALYRQAVNETPGSPMFVMKVVTDARHLEVQLLADQYGTNITLFGRDCSIQRRHQKIIEEAPVTITKPETFQRMERAAIRLGELVGYVSAGTVEYLYSPKDDKFYFLELNPRLQVEHPTTEMISGVNLPATQLQIAMGIPMHMISDIRKLYGLDPTGTSYIDFKNLKRPSPKGHCISCRITSEDPNEGFKPSTGKIHELNFRSSSNVWGYFSVGNNGAIHSFSDSQFGHIFAVGNDRQDAKQNMVLALKDFSIRGEFKTPIEYLIELLETRDFESNNISTGWLDDLILKNLSSDSKLDPTLAIICGAAMKAYVFTEKVRNKYLELLRRGQVPPKDFLKTKFPVDFIFDNNRYLFNVAQSSEEQFILSINKSQCEVNVQKLSSDCLLISVDGKCHTVYWKDDIRGTRLSIDSNTIFLEAELNPTQVISPTPGKLVKYLVRSGDHVFAGQQYAEIEIMKMQMPLVAKSDGVIELLRQPGSIIEAGDVIAKLTLDSPSKANESSLYRGELPVLGPPLIEGSRPNHKLRVLINRLENILNGYHENSGIETTLKELIKILRDGRLPYSEWDSQISTVRNRLPRQLNEGLGNLVKKSVSFPAKELHKLMKRYLEENTNDHVVYVALQPLLKISERYSEGLANHECEIFLKLIKKYYAVEKIFENHDIHEERNLLNLRRKDLTNLKKILCISLSHANVVAKNKLVTAILHEYEPLCQDSSKMSLKFRAVIHDLASLESKWAKEVAVKARSVLLRGIFPPIKKRKEHIKTLLQLHIKDTGAENIHSRNIYSCMRDFGNLIHSNLIQLQDLFFFFGHQDTALSSIASEIYARYAYGNYQLKSIKIHKGAPDLLMSWQFSSLRNYLVNSDGESDEFTKLSKPPSTSGKSSANSFGLLVNMRALESLEKTLDEVYEQIHIPEERLSSGENSLIVNILSPIRYRSENDLIKTLKIKLHENERGLSKLKVNRITFAFIAANAPAVKFYSFDGTTYDEISQIRNMDPSYEAPLELGKMSNYKIRSLPTYDSSIRIFEGISKFTPLDKRFFVRKIINSFMYNDQKTTEENLKAEINAQVVYMLEHLGAVDISNSDLNHIFLSFNTVLNIPVHRLEEIVSTILKTHETRLFQERITDVEICISVECLETKKPAPLRLLISNKSGYVVKIETYYEKIGKNGNLILEPCSEQSHYSQKSLSLPYSVKDWLQPKRYKAQFMGTTYVYDFPGLFHQAAIQQWKRYFPKHKLNDSFFSWVELIEQNGNLIKVNREPGLNNIGMVAFEIMVQTPEYPEGRNMIVISNDITYNIGSFGPREDLFFDRVTNYARERGIPRIYLAANSGAKLGIAEELIPLFRVAWNDPSDPTKGFQYLYLAPKDMQLLKDSGKGNSVVVEHKMVYGEERYIIKAIVGFEEGLGVECLQGSGLIAGATSKAYRDIFTITAVTCRSVGIGSYLVRLGQRTIQVEDKPIILTGASAINKVLGTDIYTSNLQIGGTQIMYKNGIAHLTASNDMKAIEKIMTWLSYVPAKRDMSPPLLETMDRWDRDVDFKPAKQVPYEARWLIEGKWDSNNNFQSGLFDKDSFFETLSGWAKGVIVGRARLGGIPVGVIAVETKTIEEIIPADPANLDSSEFSVKEAGQVWYPNSAFKTAQTINDFNYGEQLPLIILANWRGFSGGQRDMYNEVLKYGSFIVDALVDYKQPILIYIPPFGELRGGSWVVIDPTINPEQMEMYADVESRGGVLEPDGVVSIKYRKEKMIETMIRLDSTYGHLRRTLTEKKLSLEKQNDLTKRLKIRERQLIPIYNQISIQFADLHDRSTRMLVKGVIRNELEWKKSRRFLYWRLRRRLNEGQVIKRLQKKTCDNKTKMKYDDLLKIVQSWYNDLDVNDDRAVVEFIERNSKKIDKNIEEFEISLLIDELKKKFEDRRGNIVLEELTRLVDSKRKR

Target ORF information:

RefSeq Version	NM_001182714.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C acetyl-CoA carboxylase HFA1 (HFA1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182714.1

ORF Insert Sequence:

ATGAGATCTA TAAGAAAATG GGCGTACGAG ACGTTCAATG ATGAAAAAAT CATTCAATTC 
GTGGTAATGG CGACACCTGA TGATTTACAC GCAAATTCGG AGTATATTAG AATGGCAGAC 
CAATATGTGC AGGTACCAGG GGGTACCAAC AACAACAATT ACGCCAACAT AGACTTAATA 
CTGGACGTGG CAGAGCAAAC GGATGTGGAT GCGGTCTGGG CTGGATGGGG CCATGCTTCT 
GAAAATCCGT GTCTTCCTGA GCTGTTAGCT AGTTCACAAA GGAAAATACT ATTCATTGGT 
CCTCCTGGAC GCGCTATGAG ATCATTGGGT GACAAGATTT CTTCCACTAT TGTAGCACAA 
AGCGCTAAAA TCCCGTGTAT CCCTTGGTCT GGTTCACATA TAGACACTAT CCATATCGAT 
AACAAGACGA ACTTTGTATC TGTGCCGGAT GATGTATATG TAAGGGGATG TTGTTCCTCA 
CCTGAAGATG CTTTAGAAAA GGCTAAATTA ATAGGATTTC CTGTAATGAT TAAGGCATCC 
GAAGGTGGTG GAGGTAAGGG CATTAGGCGA GTAGATAATG AGGATGATTT TATTGCATTA 
TATCGCCAAG CAGTGAATGA GACACCTGGG TCGCCTATGT TTGTTATGAA AGTTGTCACT 
GATGCTCGTC ACTTAGAGGT ACAGTTATTA GCTGACCAAT ATGGCACTAA CATTACATTG 
TTTGGGAGAG ACTGTTCCAT ACAAAGGCGG CACCAAAAGA TTATAGAAGA GGCACCAGTG 
ACAATAACCA AGCCTGAAAC GTTTCAAAGG ATGGAACGCG CAGCAATTCG TCTAGGTGAA 
TTGGTAGGTT ATGTTTCTGC GGGCACTGTC GAATACTTAT ATTCACCAAA AGATGATAAA 
TTTTACTTTT TAGAACTGAA TCCGAGACTA CAAGTAGAGC ATCCAACGAC AGAAATGATA 
TCTGGCGTAA ACCTTCCTGC CACTCAACTG CAAATCGCCA TGGGCATTCC TATGCACATG 
ATAAGTGATA TCAGAAAACT TTATGGTTTA GATCCAACGG GAACTTCGTA TATTGATTTT 
AAAAATTTAA AGAGACCCTC GCCAAAAGGC CATTGTATTT CATGCAGGAT CACTTCAGAA 
GATCCTAATG AAGGTTTCAA GCCCTCCACT GGGAAAATAC ATGAGCTCAA TTTTCGTTCT 
TCTTCCAATG TTTGGGGTTA CTTCTCAGTA GGAAATAATG GTGCTATTCA CTCATTTTCA 
GATTCCCAAT TTGGGCACAT TTTTGCTGTA GGAAACGATA GGCAAGATGC AAAGCAAAAC 
ATGGTTTTAG CTCTAAAAGA TTTTTCCATC CGAGGAGAAT TCAAAACCCC CATAGAGTAC 
CTGATAGAGC TATTAGAAAC TCGGGACTTT GAGAGTAATA ACATATCGAC TGGTTGGTTA 
GATGATTTGA TTTTGAAAAA TTTATCTTCC GATAGCAAAC TAGATCCAAC GCTCGCTATT 
ATCTGTGGTG CCGCAATGAA AGCATACGTT TTCACAGAAA AGGTGAGGAA TAAGTATTTG 
GAATTATTGC GGAGGGGCCA AGTTCCACCT AAAGATTTTC TTAAAACGAA GTTTCCTGTT 
GACTTCATTT TCGATAATAA TAGATACTTG TTCAATGTTG CTCAATCATC TGAAGAACAA 
TTTATTCTTT CTATCAATAA GTCTCAATGT GAAGTTAATG TTCAAAAATT GTCCAGTGAC 
TGCTTGTTGA TCTCCGTTGA CGGTAAATGC CATACAGTTT ATTGGAAGGA CGATATCAGA 
GGTACAAGAC TTTCGATAGA CTCCAATACC ATATTTTTAG AAGCTGAACT CAATCCCACT 
CAAGTGATCT CTCCAACTCC GGGGAAATTG GTGAAATATT TGGTCCGAAG TGGTGATCAC 
GTTTTTGCTG GACAGCAATA TGCAGAAATA GAAATAATGA AAATGCAGAT GCCACTAGTA 
GCGAAAAGTG ATGGTGTAAT TGAGTTACTA AGACAGCCCG GTTCCATAAT TGAGGCTGGT 
GATGTCATCG CAAAATTGAC TTTGGATTCG CCGTCCAAAG CTAACGAATC GTCTTTATAC 
CGCGGAGAAT TACCTGTTTT AGGTCCACCG CTAATAGAGG GTAGCCGACC AAACCATAAG 
CTCAGAGTCT TAATAAATAG GTTAGAAAAT ATTCTCAATG GATATCATGA AAACTCTGGA 
ATAGAAACTA CTCTAAAAGA GTTGATAAAA ATATTGAGAG ATGGTAGGCT TCCTTATTCA 
GAATGGGATT CCCAAATTTC TACGGTACGC AATAGACTAC CAAGGCAATT GAATGAGGGG 
CTGGGAAATC TAGTCAAGAA ATCTGTTTCT TTTCCTGCAA AGGAACTGCA CAAATTAATG 
AAGCGCTACT TGGAAGAAAA TACAAATGAT CATGTAGTTT ATGTTGCCTT ACAGCCACTT 
CTTAAAATTA GTGAAAGGTA TAGCGAAGGT TTAGCTAATC ACGAATGTGA AATTTTTTTA 
AAGTTGATTA AAAAGTACTA TGCTGTTGAG AAAATTTTTG AAAATCATGA TATACATGAA 
GAAAGAAACT TACTAAATCT GCGGAGGAAA GACCTTACAA ACTTGAAAAA AATTTTGTGC 
ATAAGTTTAT CGCATGCTAA CGTAGTCGCA AAGAACAAGT TAGTAACTGC AATATTGCAT 
GAATACGAGC CATTGTGCCA GGATTCCTCT AAGATGTCTT TAAAATTCAG GGCTGTTATA 
CATGATTTGG CAAGTTTGGA ATCTAAGTGG GCTAAAGAGG TTGCTGTAAA GGCAAGATCA 
GTGCTACTCA GAGGGATTTT CCCTCCCATA AAGAAAAGAA AAGAGCATAT TAAAACTCTC 
CTGCAATTGC ACATAAAGGA TACTGGTGCC GAAAACATTC ACAGCAGGAA CATATATTCC 
TGTATGAGGG ATTTTGGTAA TTTAATACAT TCAAATCTGA TACAACTTCA GGATTTGTTC 
TTTTTTTTTG GCCATCAAGA TACGGCTCTT TCCAGTATAG CATCTGAAAT TTATGCAAGG 
TATGCCTACG GCAATTATCA ATTAAAAAGT ATTAAGATTC ATAAAGGAGC GCCTGATTTA 
CTAATGTCAT GGCAATTCAG CTCATTAAGA AATTATTTAG TCAATTCTGA TGGTGAGAGT 
GATGAGTTTA CAAAACTTTC TAAACCTCCC TCAACATCAG GTAAGAGCTC AGCAAATAGT 
TTTGGTCTTC TTGTCAACAT GCGTGCGCTT GAATCTCTGG AAAAGACATT AGACGAGGTA 
TACGAACAAA TTCATATTCC TGAGGAAAGA CTTTCCAGCG GAGAGAACTC TCTTATTGTT 
AATATTTTAT CTCCTATTCG TTACAGAAGT GAAAATGATC TAATTAAAAC TTTAAAAATT 
AAACTTCATG AAAATGAGAG AGGTCTATCC AAGCTCAAGG TTAATCGTAT TACATTTGCA 
TTTATCGCCG CGAATGCGCC CGCTGTTAAA TTTTACTCCT TTGATGGAAC TACGTACGAT 
GAAATCTCTC AAATAAGAAA TATGGATCCA TCCTATGAAG CACCGTTAGA GTTAGGAAAA 
ATGTCGAACT ATAAAATCAG ATCACTACCT ACATACGATA GTAGTATACG CATTTTTGAA 
GGTATTAGCA AATTTACGCC GCTAGATAAA AGGTTCTTTG TCAGGAAAAT CATAAATTCC 
TTCATGTATA ATGATCAAAA AACAACCGAA GAAAACTTGA AAGCGGAAAT CAATGCTCAA 
GTGGTTTATA TGTTAGAACA TCTAGGAGCA GTTGACATCT CAAATTCAGA CTTGAATCAT 
ATTTTTTTAA GTTTCAATAC AGTTCTTAAC ATACCAGTAC ATCGTCTCGA GGAAATTGTG 
AGTACAATTC TAAAGACTCA CGAAACCAGA TTGTTTCAAG AAAGAATCAC AGATGTAGAA 
ATTTGCATCT CTGTTGAGTG CCTAGAAACA AAGAAGCCAG CCCCGCTTAG ATTACTTATT 
TCTAATAAAT CTGGGTATGT GGTAAAAATT GAGACATATT ACGAAAAGAT AGGGAAAAAT 
GGGAATCTGA TTTTGGAACC GTGTAGTGAG CAGAGCCATT ATAGCCAGAA ATCTCTCTCT 
CTTCCTTACT CGGTCAAGGA TTGGCTACAA CCTAAAAGGT ACAAAGCTCA ATTCATGGGT 
ACAACATATG TGTACGATTT CCCAGGTCTG TTTCATCAAG CTGCAATCCA ACAGTGGAAA 
AGGTATTTTC CAAAACATAA GCTGAATGAC AGTTTTTTTA GTTGGGTTGA ATTGATAGAA 
CAAAACGGTA ATTTAATAAA AGTAAACAGG GAGCCAGGCC TTAATAATAT AGGGATGGTT 
GCTTTTGAGA TTATGGTTCA GACACCTGAA TATCCTGAAG GGCGTAACAT GATCGTGATT 
TCTAATGATA TTACCTACAA TATTGGATCT TTTGGACCGA GAGAAGATTT GTTTTTTGAT 
AGGGTCACAA ATTATGCAAG AGAGAGAGGG ATCCCGAGGA TATACTTGGC GGCGAATTCA 
GGAGCTAAAT TGGGTATAGC CGAAGAGCTG ATCCCTCTAT TTCGTGTAGC ATGGAATGAC 
CCCTCTGATC CAACAAAGGG TTTCCAGTAC TTATACTTAG CTCCAAAAGA CATGCAGCTA 
CTGAAAGATT CTGGGAAAGG AAATTCGGTT GTTGTTGAAC ACAAGATGGT ATACGGTGAA 
GAGAGATATA TTATTAAAGC AATAGTCGGA TTCGAAGAGG GTTTAGGTGT TGAATGTTTA 
CAGGGCTCGG GTTTAATTGC TGGTGCCACT TCGAAAGCGT ATAGAGACAT TTTCACTATT 
ACTGCTGTTA CTTGTCGGTC CGTTGGTATA GGTTCCTATC TGGTCAGACT AGGACAACGT 
ACTATTCAGG TGGAGGATAA GCCTATCATA CTGACGGGTG CATCGGCGAT TAATAAAGTT 
TTGGGTACCG ATATCTATAC ATCTAACCTA CAAATTGGCG GAACCCAAAT CATGTATAAA 
AACGGAATAG CGCATTTAAC AGCCAGTAAT GATATGAAAG CCATCGAAAA AATAATGACA 
TGGTTATCAT ATGTCCCGGC GAAAAGAGAT ATGAGTCCTC CACTTCTTGA AACTATGGAT 
AGATGGGATA GGGATGTAGA CTTCAAACCT GCCAAGCAAG TGCCATATGA GGCAAGGTGG 
TTGATAGAGG GTAAATGGGA CTCAAATAAC AACTTCCAGT CAGGCCTATT TGATAAGGAT 
TCGTTTTTTG AGACATTATC TGGATGGGCC AAAGGTGTAA TAGTTGGAAG AGCACGTCTT 
GGAGGTATTC CCGTGGGTGT TATTGCGGTA GAAACTAAGA CTATCGAAGA AATAATCCCC 
GCTGACCCAG CTAATCTGGA TTCTTCAGAG TTTTCCGTTA AAGAAGCAGG ACAGGTGTGG 
TATCCAAATT CCGCGTTCAA AACAGCTCAA ACTATAAATG ATTTTAACTA TGGTGAGCAA 
TTACCATTGA TTATCTTAGC CAATTGGAGG GGATTTTCTG GCGGTCAAAG GGATATGTAC 
AATGAAGTAC TAAAGTACGG GTCTTTTATT GTTGACGCTC TGGTTGACTA CAAACAACCC 
ATACTGATAT ACATTCCGCC CTTTGGTGAA TTAAGGGGCG GATCATGGGT TGTTATAGAT 
CCAACTATTA ATCCTGAACA AATGGAAATG TATGCCGATG TTGAATCTAG GGGAGGTGTG 
TTAGAACCTG ACGGAGTAGT TAGCATAAAA TACCGTAAGG AGAAAATGAT AGAGACGATG 
ATTCGATTAG ACTCCACATA TGGACATTTG AGAAGAACGT TGACAGAAAA AAAGTTATCT 
TTGGAAAAAC AAAATGATCT TACGAAGAGA TTGAAAATAA GAGAGAGACA GTTGATACCA 
ATTTACAATC AAATCAGCAT ACAGTTTGCA GATTTACATG ATAGATCGAC TAGGATGCTA 
GTTAAAGGAG TAATCCGAAA TGAGTTGGAA TGGAAAAAGT CACGCAGATT TTTATATTGG 
AGACTGAGAA GGAGGTTGAA CGAGGGACAA GTGATCAAAA GACTGCAAAA AAAAACATGT 
GATAACAAAA CGAAAATGAA GTACGACGAC CTGTTGAAAA TAGTTCAGTC ATGGTATAAC 
GATCTGGATG TTAATGATGA CAGAGCAGTA GTGGAGTTCA TAGAAAGAAA TTCGAAAAAA 
ATTGACAAGA ACATTGAAGA GTTTGAGATC TCGCTGTTGA TCGATGAGCT TAAGAAAAAA 
TTTGAAGACA GAAGGGGAAA CATTGTCCTT GAAGAGCTAA CTAGGTTGGT GGACAGTAAG 
CGAAAGAGAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Nature387(6632 Suppl)90-3(1997 May)
Bowman S,Churcher C,Badcock K,Brown D,Chillingworth T,Connor R,Dedman K,Devlin K,Gentles S,Hamlin N,Hunt S,Jagels K,Lye G,Moule S,Odell C,Pearson D,Rajandream M,Rice P,Skelton J,Walsh S,Whitehead S,Barrell B

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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HFA1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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