SGS1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	SGS1
Entrez Gene ID	855228
Full Name	ATP-dependent DNA helicase SGS1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182696.1	NP_013915.1	ATP-dependent DNA helicase SGS1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Bowman S, Churcher C, Badcock K, Brown D, Chillingworth T, Connor R, Dedman K, Devlin K, Gentles S, Hamlin N, Hunt S, Jagels K, Lye G, Moule S, Odell C, Pearson D, Rajandream M, Rice P, Skelton J, Walsh S, Whitehead S, Barrell B
Nature387(6632 Suppl)90-3(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Sgs1 and BLM regulate R-loop-associated genome instability.
Title: RECQ-like helicases Sgs1 and BLM regulate R-loop-associated genome instability.

Recently, we and others have uncover a direct link between the Smc5/6 complex and Sgs1. Most roles of Sgs1 during recombination, including DNA end resection, Holiday junction dissolution, and crossover suppression, are regulated through Mms21-dependent SUMOylation
Title: Smc5/6 complex regulates Sgs1 recombination functions.

Although Top3 and Sgs1 interact without sumoylation, sumoylated forms of Sgs1, particularly those containing K621 conjugates, are preferentially enriched in complexes with Top3.
Title: Smc5/6 Mediated Sumoylation of the Sgs1-Top3-Rmi1 Complex Promotes Removal of Recombination Intermediates.

Sgs1 mutants impaired in recognition of SUMOylated Smc5/6 (sgs1-SIMDelta) or SUMO-dead alleles (sgs1-KR) exhibit unprocessed HJs at damaged replication forks, increased crossover frequencies during double-strand break repair, and severe impairment in DNA end resection
Title: Sgs1's roles in DNA end resection, HJ dissolution, and crossover suppression require a two-step SUMO regulation dependent on Smc5/6.

Double deletion of Sgs1 and Mph1 abolishes the REC-mediated lag observed during the repair of large gaps and break-induced replication substrates.
Title: Sgs1 and Mph1 Helicases Enforce the Recombination Execution Checkpoint During DNA Double-Strand Break Repair in Saccharomyces cerevisiae.

The following SGS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SGS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04185	NM_001182696.1 Latest version!	Saccharomyces cerevisiae S288C ATP-dependent DNA helicase SGS1 (SGS1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SGS1 ( NM_001182696.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013915.1 Saccharomyces cerevisiae S288C ATP-dependent DNA helicase SGS1 (SGS1), partial mRNA.

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CloneID	OSi04185
Clone ID Related Accession (Same CDS sequence)	NM_001182696.1
Accession Version	NM_001182696.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	ATP-dependent DNA helicase SGS1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001145). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGTGACGA AGCCGTCACA TAACTTAAGA AGGGAGCACA AATGGTTAAA GGAAACGGCG 
ACTTTACAGG AAGACAAAGA TTTCGTATTC CAGGCTATCC AAAAGCACAT CGCGAACAAA 
AGGCCTAAGA CAAATTCGCC ACCAACAACG CCATCCAAAG ATGAATGTGG ACCAGGAACA 
ACAAACTTTA TAACCAGCAT TCCTGCATCT GGACCAACGA ATACTGCTAC GAAACAACAT 
GAAGTCATGC AAACTTTGTC GAACGATACA GAATGGCTCT CGTACACTGC CACATCGAAT 
CAATATGCTG ACGTACCCAT GGTTGATATA CCAGCCAGCA CAAGTGTTGT ATCGAACCCG 
AGGACGCCCA ATGGCTCGAA AACTCACAAT TTCAATACTT TTCGACCGCA CATGGCTAGT 
TCTCTTGTCG AAAATGACAG TAGCAGAAAT CTAGGTAGTA GAAATAACAA TAAAAGCGTA 
ATCGATAATT CAAGTATAGG CAAACAGCTC GAAAATGATA TAAAATTAGA GGTGATCCGC 
CTTCAAGGCT CCCTAATTAT GGCATTGAAA GAGCAATCGA AATTATTGCT ACAGAAGTGT 
AGTATTATCG AATCCACATC ATTGTCAGAG GACGCTAAAA GGCTACAGTT AAGTAGGGAT 
ATACGGCCTC AGTTATCAAA TATGTCGATA CGAATAGATA GTCTGGAAAA GGAGATCATA 
AAAGCCAAAA AAGATGGAAT GTCAAAAGAT CAAAGTAAAG GTCGCAGTCA AGTTTCTTCA 
CAAGATGATA ATATCATATC AAGCATTCTG CCCAGCCCCT TGGAATACAA TACATCTTCC 
AGGAATTCAA ACCTGACAAG TACTACTGCG ACTACCGTCA CTAAGGCATT AGCCATCACT 
GGTGCGAAGC AAAATATTAC TAATAACACC GGTAAGAATA GCAATAATGA TAGTAATAAC 
GATGATTTGA TTCAAGTCCT GGATGATGAG GATGATATAG ACTGTGACCC TCCTGTAATA 
TTGAAGGAGG GGGCTCCACA TTCTCCCGCA TTCCCGCATC TCCACATGAC GTCAGAAGAA 
CAAGATGAAC TTACAAGAAG GAGAAATATG CGTTCAAGAG AACCAGTCAA CTACAGAATA 
CCTGATAGGG ATGATCCTTT TGACTATGTT ATGGGTAAAT CTTTGAGGGA CGATTATCCG 
GATGTCGAAA GAGAAGAAGA TGAGTTAACA ATGGAGGCAG AAGATGATGC CCATTCCAGC 
TACATGACTA CTAGAGATGA AGAAAAAGAA GAAAACGAAT TACTAAATCA AAGCGATTTT 
GATTTTGTGG TAAACGACGA CCTAGACCCA ACTCAAGACA CAGATTATCA TGATAATATG 
GATGTTAGTG CAAATATTCA GGAAAGTTCT CAAGAAGGTG ACACTAGGTC CACAATTACC 
TTGTCGCAAA ATAAAAATGT TCAAGTTATT TTATCATCTC CCACAGCACA GAGCGTTCCC 
TCAAATGGCC AAAATCAAAT AGGCGTGGAG CATATTGACT TGTTGGAAGA TGATCTGGAA 
AAGGACGCAA TTTTGGATGA TAGCATGAGT TTCTCCTTTG GCCGTCAACA CATGCCCATG 
TCTCATTCTG ATCTAGAGTT GATAGACAGC GAAAAAGAAA ATGAGGATTT TGAAGAAGAT 
AATAACAATA ACGGTATCGA GTACCTATCA GATAGCGATT TAGAAAGATT TGACGAAGAA 
AGAGAGAATA GAACCCAAGT AGCAGATATC CAGGAACTAG ACAATGACCT GAAAATAATA 
ACAGAAAGGA AGCTTACAGG TGACAATGAA CACCCACCAC CATCTTGGTC TCCCAAAATA 
AAAAGGGAGA AATCCAGTGT TAGTCAAAAG GATGAGGAAG ACGATTTTGA TGACGATTTT 
TCATTAAGTG ATATAGTGAG TAAATCCAAT TTATCTTCTA AGACGAATGG TCCAACCTAT 
CCTTGGTCTG ATGAAGTTTT ATATCGTTTA CATGAAGTCT TTAAACTGCC TGGTTTTAGA 
CCTAACCAAC TAGAGGCTGT AAATGCAACT TTGCAAGGTA AGGATGTTTT TGTTCTTATG 
CCAACAGGGG GTGGTAAATC TCTTTGCTAT CAACTTCCTG CAGTGGTGAA ATCGGGTAAA 
ACACATGGTA CTACTATTGT CATCTCTCCG CTAATTTCCC TGATGCAAGA TCAAGTGGAA 
CATTTATTGA ATAAAAATAT TAAGGCGAGC ATGTTCAGTT CGAGGGGTAC TGCCGAGCAA 
AGACGACAAA CTTTCAATTT ATTTATTAAT GGATTATTGG ATTTAGTTTA CATATCTCCT 
GAGATGATCA GTGCCTCAGA ACAATGCAAG AGAGCTATCA GTAGATTATA CGCAGACGGT 
AAGTTGGCTC GTATTGTTGT AGATGAAGCA CATTGTGTTT CTAACTGGGG CCACGATTTC 
AGGCCTGATT ATAAAGAATT AAAATTTTTC AAAAGAGAAT ACCCTGATAT TCCAATGATT 
GCTTTAACTG CAACTGCAAG TGAACAAGTC AGAATGGACA TCATTCACAA TTTAGAACTA 
AAGGAACCTG TTTTCCTAAA ACAAAGTTTT AATAGAACAA ATTTGTATTA CGAAGTAAAC 
AAGAAGACCA AAAATACCAT TTTTGAAATC TGTGATGCGG TTAAATCTAG GTTCAAAAAT 
CAAACGGGTA TAATATATTG CCACTCCAAG AAATCATGCG AGCAAACATC AGCCCAAATG 
CAAAGAAATG GCATCAAGTG TGCATATTAC CATGCAGGCA TGGAGCCTGA TGAAAGATTA 
AGTGTACAGA AGGCATGGCA GGCGGATGAG ATACAAGTCA TTTGTGCTAC TGTTGCTTTC 
GGAATGGGTA TTGATAAACC TGATGTGAGA TTTGTTTACC ACTTTACCGT TCCCCGAACA 
TTAGAAGGCT ATTATCAAGA AACCGGCCGT GCTGGAAGAG ATGGGAACTA TTCATATTGT 
ATTACCTACT TTTCATTCAG GGACATTAGA ACCATGCAGA CAATGATCCA GAAGGATAAG 
AACTTAGACA GAGAGAATAA GGAAAAACAT CTGAATAAAT TACAGCAAGT AATGGCATAC 
TGTGACAACG TTACTGACTG CAGAAGAAAG TTAGTTTTAT CTTATTTCAA TGAGGATTTT 
GACTCCAAAC TGTGTCATAA AAACTGTGAT AATTGTAGAA ATAGCGCCAA CGTGATAAAC 
GAGGAAAGGG ATGTTACAGA ACCTGCCAAG AAGATTGTAA AATTAGTGGA AAGTATCCAA 
AATGAAAGAG TCACAATAAT TTATTGCCAA GATGTCTTTA AAGGTTCGAG AAGCTCCAAA 
ATTGTTCAGG CTAACCATGA CACCTTAGAG GAGCATGGTA TTGGTAAATC CATGCAAAAA 
TCAGAAATCG AAAGGATTTT CTTCCATTTG ATTACGATCC GAGTTTTACA AGAGTATTCA 
ATAATGAACA ATAGCGGTTT TGCTTCAAGC TATGTGAAAG TTGGTCCCAA TGCTAAGAAA 
TTGCTGACTG GAAAAATGGA GATAAAGATG CAATTTACAA TATCAGCACC AAACTCCCGT 
CCCTCGACAT CTTCTAGCTT TCAAGCAAAT GAAGATAATA TACCAGTTAT CGCGCAGAAA 
TCAACAACCA TTGGAGGAAA TGTGGCTGCT AATCCACCGC GCTTTATAAG TGCGAAGGAA 
CACCTAAGAT CGTATACATA CGGCGGTTCG ACCATGGGTA GCTCACATCC GATCACTTTG 
AAAAACACAA GCGATTTACG CTCGACACAA GAACTTAATA ATCTGCGAAT GACATACGAA 
CGTCTGAGGG AATTATCTTT AAATTTAGGA AATAGAATGG TTCCTCCAGT TGGGAACTTT 
ATGCCTGACA GTATTTTAAA AAAGATGGCA GCAATATTAC CAATGAATGA TTCGGCTTTT 
GCAACTTTAG GCACAGTGGA GGACAAATAT CGCCGAAGGT TCAAGTACTT TAAAGCCACG 
ATAGCAGATC TTAGCAAAAA GAGATCAAGC GAGGATCATG AAAAATACGA CACAATACTA 
AATGATGAAT TTGTGAACAG GGCCGCGGCT TCCAGCAATG GGATTGCCCA AAGTACTGGA 
ACAAAATCTA AGTTTTTTGG CGCCAATTTA AACGAAGCCA AAGAAAATGA ACAAATTATC 
AATCAAATCA GACAAAGCCA ATTACCCAAA AATACCACAA GCAGCAAATC AGGTACAAGG 
TCCATCAGTA AGTCGTCCAA GAAGTCTGCT AATGGGAGAC GAGGTTTTAG AAATTACCGA 
GGTCACTACA GAGGAAGAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013915.1
CDS	1..4344
Translation	MVTKPSHNLRREHKWLKETATLQEDKDFVFQAIQKHIANKRPKTNSPPTTPSKDECGPGTTNFITSIPASGPTNTATKQHEVMQTLSNDTEWLSYTATSNQYADVPMVDIPASTSVVSNPRTPNGSKTHNFNTFRPHMASSLVENDSSRNLGSRNNNKSVIDNSSIGKQLENDIKLEVIRLQGSLIMALKEQSKLLLQKCSIIESTSLSEDAKRLQLSRDIRPQLSNMSIRIDSLEKEIIKAKKDGMSKDQSKGRSQVSSQDDNIISSILPSPLEYNTSSRNSNLTSTTATTVTKALAITGAKQNITNNTGKNSNNDSNNDDLIQVLDDEDDIDCDPPVILKEGAPHSPAFPHLHMTSEEQDELTRRRNMRSREPVNYRIPDRDDPFDYVMGKSLRDDYPDVEREEDELTMEAEDDAHSSYMTTRDEEKEENELLNQSDFDFVVNDDLDPTQDTDYHDNMDVSANIQESSQEGDTRSTITLSQNKNVQVILSSPTAQSVPSNGQNQIGVEHIDLLEDDLEKDAILDDSMSFSFGRQHMPMSHSDLELIDSEKENEDFEEDNNNNGIEYLSDSDLERFDEERENRTQVADIQELDNDLKIITERKLTGDNEHPPPSWSPKIKREKSSVSQKDEEDDFDDDFSLSDIVSKSNLSSKTNGPTYPWSDEVLYRLHEVFKLPGFRPNQLEAVNATLQGKDVFVLMPTGGGKSLCYQLPAVVKSGKTHGTTIVISPLISLMQDQVEHLLNKNIKASMFSSRGTAEQRRQTFNLFINGLLDLVYISPEMISASEQCKRAISRLYADGKLARIVVDEAHCVSNWGHDFRPDYKELKFFKREYPDIPMIALTATASEQVRMDIIHNLELKEPVFLKQSFNRTNLYYEVNKKTKNTIFEICDAVKSRFKNQTGIIYCHSKKSCEQTSAQMQRNGIKCAYYHAGMEPDERLSVQKAWQADEIQVICATVAFGMGIDKPDVRFVYHFTVPRTLEGYYQETGRAGRDGNYSYCITYFSFRDIRTMQTMIQKDKNLDRENKEKHLNKLQQVMAYCDNVTDCRRKLVLSYFNEDFDSKLCHKNCDNCRNSANVINEERDVTEPAKKIVKLVESIQNERVTIIYCQDVFKGSRSSKIVQANHDTLEEHGIGKSMQKSEIERIFFHLITIRVLQEYSIMNNSGFASSYVKVGPNAKKLLTGKMEIKMQFTISAPNSRPSTSSSFQANEDNIPVIAQKSTTIGGNVAANPPRFISAKEHLRSYTYGGSTMGSSHPITLKNTSDLRSTQELNNLRMTYERLRELSLNLGNRMVPPVGNFMPDSILKKMAAILPMNDSAFATLGTVEDKYRRRFKYFKATIADLSKKRSSEDHEKYDTILNDEFVNRAAASSNGIAQSTGTKSKFFGANLNEAKENEQIINQIRQSQLPKNTTSSKSGTRSISKSSKKSANGRRGFRNYRGHYRGRK

Target ORF information:

RefSeq Version	NM_001182696.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C ATP-dependent DNA helicase SGS1 (SGS1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182696.1

ORF Insert Sequence:

ATGGTGACGA AGCCGTCACA TAACTTAAGA AGGGAGCACA AATGGTTAAA GGAAACGGCG 
ACTTTACAGG AAGACAAAGA TTTCGTATTC CAGGCTATCC AAAAGCACAT CGCGAACAAA 
AGGCCTAAGA CAAATTCGCC ACCAACAACG CCATCCAAAG ATGAATGTGG ACCAGGAACA 
ACAAACTTTA TAACCAGCAT TCCTGCATCT GGACCAACGA ATACTGCTAC GAAACAACAT 
GAAGTCATGC AAACTTTGTC GAACGATACA GAATGGCTCT CGTACACTGC CACATCGAAT 
CAATATGCTG ACGTACCCAT GGTTGATATA CCAGCCAGCA CAAGTGTTGT ATCGAACCCG 
AGGACGCCCA ATGGCTCGAA AACTCACAAT TTCAATACTT TTCGACCGCA CATGGCTAGT 
TCTCTTGTCG AAAATGACAG TAGCAGAAAT CTAGGTAGTA GAAATAACAA TAAAAGCGTA 
ATCGATAATT CAAGTATAGG CAAACAGCTC GAAAATGATA TAAAATTAGA GGTGATCCGC 
CTTCAAGGCT CCCTAATTAT GGCATTGAAA GAGCAATCGA AATTATTGCT ACAGAAGTGT 
AGTATTATCG AATCCACATC ATTGTCAGAG GACGCTAAAA GGCTACAGTT AAGTAGGGAT 
ATACGGCCTC AGTTATCAAA TATGTCGATA CGAATAGATA GTCTGGAAAA GGAGATCATA 
AAAGCCAAAA AAGATGGAAT GTCAAAAGAT CAAAGTAAAG GTCGCAGTCA AGTTTCTTCA 
CAAGATGATA ATATCATATC AAGCATTCTG CCCAGCCCCT TGGAATACAA TACATCTTCC 
AGGAATTCAA ACCTGACAAG TACTACTGCG ACTACCGTCA CTAAGGCATT AGCCATCACT 
GGTGCGAAGC AAAATATTAC TAATAACACC GGTAAGAATA GCAATAATGA TAGTAATAAC 
GATGATTTGA TTCAAGTCCT GGATGATGAG GATGATATAG ACTGTGACCC TCCTGTAATA 
TTGAAGGAGG GGGCTCCACA TTCTCCCGCA TTCCCGCATC TCCACATGAC GTCAGAAGAA 
CAAGATGAAC TTACAAGAAG GAGAAATATG CGTTCAAGAG AACCAGTCAA CTACAGAATA 
CCTGATAGGG ATGATCCTTT TGACTATGTT ATGGGTAAAT CTTTGAGGGA CGATTATCCG 
GATGTCGAAA GAGAAGAAGA TGAGTTAACA ATGGAGGCAG AAGATGATGC CCATTCCAGC 
TACATGACTA CTAGAGATGA AGAAAAAGAA GAAAACGAAT TACTAAATCA AAGCGATTTT 
GATTTTGTGG TAAACGACGA CCTAGACCCA ACTCAAGACA CAGATTATCA TGATAATATG 
GATGTTAGTG CAAATATTCA GGAAAGTTCT CAAGAAGGTG ACACTAGGTC CACAATTACC 
TTGTCGCAAA ATAAAAATGT TCAAGTTATT TTATCATCTC CCACAGCACA GAGCGTTCCC 
TCAAATGGCC AAAATCAAAT AGGCGTGGAG CATATTGACT TGTTGGAAGA TGATCTGGAA 
AAGGACGCAA TTTTGGATGA TAGCATGAGT TTCTCCTTTG GCCGTCAACA CATGCCCATG 
TCTCATTCTG ATCTAGAGTT GATAGACAGC GAAAAAGAAA ATGAGGATTT TGAAGAAGAT 
AATAACAATA ACGGTATCGA GTACCTATCA GATAGCGATT TAGAAAGATT TGACGAAGAA 
AGAGAGAATA GAACCCAAGT AGCAGATATC CAGGAACTAG ACAATGACCT GAAAATAATA 
ACAGAAAGGA AGCTTACAGG TGACAATGAA CACCCACCAC CATCTTGGTC TCCCAAAATA 
AAAAGGGAGA AATCCAGTGT TAGTCAAAAG GATGAGGAAG ACGATTTTGA TGACGATTTT 
TCATTAAGTG ATATAGTGAG TAAATCCAAT TTATCTTCTA AGACGAATGG TCCAACCTAT 
CCTTGGTCTG ATGAAGTTTT ATATCGTTTA CATGAAGTCT TTAAACTGCC TGGTTTTAGA 
CCTAACCAAC TAGAGGCTGT AAATGCAACT TTGCAAGGTA AGGATGTTTT TGTTCTTATG 
CCAACAGGGG GTGGTAAATC TCTTTGCTAT CAACTTCCTG CAGTGGTGAA ATCGGGTAAA 
ACACATGGTA CTACTATTGT CATCTCTCCG CTAATTTCCC TGATGCAAGA TCAAGTGGAA 
CATTTATTGA ATAAAAATAT TAAGGCGAGC ATGTTCAGTT CGAGGGGTAC TGCCGAGCAA 
AGACGACAAA CTTTCAATTT ATTTATTAAT GGATTATTGG ATTTAGTTTA CATATCTCCT 
GAGATGATCA GTGCCTCAGA ACAATGCAAG AGAGCTATCA GTAGATTATA CGCAGACGGT 
AAGTTGGCTC GTATTGTTGT AGATGAAGCA CATTGTGTTT CTAACTGGGG CCACGATTTC 
AGGCCTGATT ATAAAGAATT AAAATTTTTC AAAAGAGAAT ACCCTGATAT TCCAATGATT 
GCTTTAACTG CAACTGCAAG TGAACAAGTC AGAATGGACA TCATTCACAA TTTAGAACTA 
AAGGAACCTG TTTTCCTAAA ACAAAGTTTT AATAGAACAA ATTTGTATTA CGAAGTAAAC 
AAGAAGACCA AAAATACCAT TTTTGAAATC TGTGATGCGG TTAAATCTAG GTTCAAAAAT 
CAAACGGGTA TAATATATTG CCACTCCAAG AAATCATGCG AGCAAACATC AGCCCAAATG 
CAAAGAAATG GCATCAAGTG TGCATATTAC CATGCAGGCA TGGAGCCTGA TGAAAGATTA 
AGTGTACAGA AGGCATGGCA GGCGGATGAG ATACAAGTCA TTTGTGCTAC TGTTGCTTTC 
GGAATGGGTA TTGATAAACC TGATGTGAGA TTTGTTTACC ACTTTACCGT TCCCCGAACA 
TTAGAAGGCT ATTATCAAGA AACCGGCCGT GCTGGAAGAG ATGGGAACTA TTCATATTGT 
ATTACCTACT TTTCATTCAG GGACATTAGA ACCATGCAGA CAATGATCCA GAAGGATAAG 
AACTTAGACA GAGAGAATAA GGAAAAACAT CTGAATAAAT TACAGCAAGT AATGGCATAC 
TGTGACAACG TTACTGACTG CAGAAGAAAG TTAGTTTTAT CTTATTTCAA TGAGGATTTT 
GACTCCAAAC TGTGTCATAA AAACTGTGAT AATTGTAGAA ATAGCGCCAA CGTGATAAAC 
GAGGAAAGGG ATGTTACAGA ACCTGCCAAG AAGATTGTAA AATTAGTGGA AAGTATCCAA 
AATGAAAGAG TCACAATAAT TTATTGCCAA GATGTCTTTA AAGGTTCGAG AAGCTCCAAA 
ATTGTTCAGG CTAACCATGA CACCTTAGAG GAGCATGGTA TTGGTAAATC CATGCAAAAA 
TCAGAAATCG AAAGGATTTT CTTCCATTTG ATTACGATCC GAGTTTTACA AGAGTATTCA 
ATAATGAACA ATAGCGGTTT TGCTTCAAGC TATGTGAAAG TTGGTCCCAA TGCTAAGAAA 
TTGCTGACTG GAAAAATGGA GATAAAGATG CAATTTACAA TATCAGCACC AAACTCCCGT 
CCCTCGACAT CTTCTAGCTT TCAAGCAAAT GAAGATAATA TACCAGTTAT CGCGCAGAAA 
TCAACAACCA TTGGAGGAAA TGTGGCTGCT AATCCACCGC GCTTTATAAG TGCGAAGGAA 
CACCTAAGAT CGTATACATA CGGCGGTTCG ACCATGGGTA GCTCACATCC GATCACTTTG 
AAAAACACAA GCGATTTACG CTCGACACAA GAACTTAATA ATCTGCGAAT GACATACGAA 
CGTCTGAGGG AATTATCTTT AAATTTAGGA AATAGAATGG TTCCTCCAGT TGGGAACTTT 
ATGCCTGACA GTATTTTAAA AAAGATGGCA GCAATATTAC CAATGAATGA TTCGGCTTTT 
GCAACTTTAG GCACAGTGGA GGACAAATAT CGCCGAAGGT TCAAGTACTT TAAAGCCACG 
ATAGCAGATC TTAGCAAAAA GAGATCAAGC GAGGATCATG AAAAATACGA CACAATACTA 
AATGATGAAT TTGTGAACAG GGCCGCGGCT TCCAGCAATG GGATTGCCCA AAGTACTGGA 
ACAAAATCTA AGTTTTTTGG CGCCAATTTA AACGAAGCCA AAGAAAATGA ACAAATTATC 
AATCAAATCA GACAAAGCCA ATTACCCAAA AATACCACAA GCAGCAAATC AGGTACAAGG 
TCCATCAGTA AGTCGTCCAA GAAGTCTGCT AATGGGAGAC GAGGTTTTAG AAATTACCGA 
GGTCACTACA GAGGAAGAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Nature387(6632 Suppl)90-3(1997 May)
Bowman S,Churcher C,Badcock K,Brown D,Chillingworth T,Connor R,Dedman K,Devlin K,Gentles S,Hamlin N,Hunt S,Jagels K,Lye G,Moule S,Odell C,Pearson D,Rajandream M,Rice P,Skelton J,Walsh S,Whitehead S,Barrell B

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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SGS1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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